Search

A remarkable number of films that form part of the “arthouse“ cinemas of post-apartheid South Africa and post-revolutionary Iran put the spotlight on children and childhood. This cinematic focus on the figure of the child developed at a point in time when both countries witnessed significant political and social changes. In Iran, the Islamic Revolution of 1979 overthrew the Pahlavi monarchy and this led to the establishment of the Islamic Republic. In South Africa, in turn, the transition from apartheid to democracy in 1994 ended decades of legalised racial segregation under apartheid. This article compares and contrasts representations of children – girls in particular – within two fiction films from post-Revolutionary Iran and postapartheid South Africa – 'Ayneh/The Mirror' (1997) and 'Life, Above All' (2010). The analysis investigates representations of children, the relationships between children and adults, and the spaces, places, and roles ascribed to children. Particular attention is given to the ways in which representations of childhood and gender intersect. The article argues that the selected films represent girls as both situated within liminal spaces and as agents who subvert established social hierarchies and taboo subjects. The films thus construct girls as transformational agents in the context of two societies that have recently experienced periods of transitions and change.

Background Group B streptococcus (GBS) causes serious diseases in newborn infants, often resulting in lifelong neurologic impairments or death. Prophylactic vaccination of pregnant women prior to delivery could provide comprehensive protection, as early onset and late-onset disease and maternal complications potentially could be addressed. Methods Capsular polysaccharide conjugate vaccine GBS6 was designed using surveillance data yielded by whole-genome sequencing of a global collection of recently recovered GBS isolates responsible for invasive neonatal GBS disease. Capsular polysaccharides were isolated, oxidized using sodium periodate, and conjugated to CRM197 by reductive amination in dimethyl sulfoxide. Immune responses in mice and rhesus macaques were measured in a multiplex Luminex immunoglobulin G (IgG) assay and opsonophagocytic activity assays. Results The optimized conjugates were immunogenic, alone and in combination, in mice and rhesus macaques, inducing IgG antibodies that mediated opsonophagocytic killing. Active immunization of murine dams with GBS6 prior to mating resulted in serotype-specific protection of pups from a lethal challenge with GBS. Protection following passive administration of serotype-specific IgG monoclonal antibodies to dams demonstrated conclusively that anticapsular polysaccharide IgG alone is sufficient for protection. Conclusions The findings support the ongoing clinical evaluation of maternal GBS6 vaccination as a potential alternative method to prevent GBS disease in infants., Six-valent capsular polysaccharide conjugate vaccine GBS6 for the prevention of neonatal Group B Streptococcal (GBS) infections was evaluated in preclinical models. Vaccination with GBS6 or administration of serotype-specific IgG to pregnant dams protected pups against lethal challenges with GBS.

This article explores representations of childhood and forced migration within a selection of European screen content for and about children. Based on the findings of a research project that examined the intersections of children’s media, diversity, and forced migration in Europe (www.euroarabchildrensmedia.org), funded by the UK’s Arts and Humanities Research Council, the article highlights different ways in which ideas of borders and belonging are constructed and deconstructed in a selection of films and television programmes that feature children with an immigration background. Drawing on ideas around the “politics of pity” (Arendt), the analysis explores conditions under which narratives of otherness arise when it comes to representing forcibly displaced children within European-produced children’s screen media. It also examines screen media that destabilize borders of “us” and “the other” by emphasizing the agency of children from migration backgrounds, and revealing both the similarities and the differences between European children with immigration backgrounds and White European-born children. It is argued here that, operating according to the notions of living “together-in-difference” (Ang), “narratability” (Chouliaraki and Stolic), and “the struggle for belonging” (Kebede), these representations destabilize narratives of borders and otherness, suggesting that children with a family history of immigration “belong” to European societies in the same ways as White European-born children.

Staphylococcus aureus produces an antiphagocytic polysaccharide capsule to evade neutrophil-mediated killing. Many vaccines against encapsulated bacterial pathogens require generation of functional anti-capsular antibodies to mediate protection against infection and disease. Here it is shown that the generation of such antibody responses to S. aureus in vivo and in vitro requires the presence of O-acetyl modifications on the capsular polysaccharides. O-acetylation of S. aureus capsular polysaccharide therefore should be monitored carefully during vaccine development and production. This finding may provide additional insight into the previous failure of a S. aureus capsular polysaccharide conjugate vaccine.

Glucose-stimulated insulin secretion (GSIS) is the gold standard for β-cell function. Both experimental and clinical diabetology, i. e., preceding transplantation of isolated human islets, depend on functional testing. However, multiple factors influence GSIS rendering the comparison of different in vitro tests of glucose responsiveness difficult. This study examined the influence of bovine serum albumin (BSA)-coupled fatty acids on GSIS. Isolated islet preparations of human donors and of 12-months old mice displayed impaired GSIS in the presence of 0.5% FFA-free BSA compared to 0.5% BSA (fraction V, not deprived from fatty acids). In aged INS-1E cells, i. e. at a high passage number, GSIS became highly sensitive to FFA-free BSA. Readdition of 30 µM palmitate or 30 µM oleate to FFA-free BSA did not rescue GSIS, while the addition of 100 µM palmitate and the raise of extracellular Ca2+from 1.3 to 2.6 mM improved glucose responsiveness. A high concentration of palmitate (600 µM), which fully activates FFA1, largely restored insulin secretion. The FFA1-agonist TUG-469 also increased insulin secretion but to a lesser extent than palmitate. Glucose- and TUG-induced Ca2+oscillations were impaired in glucose-unresponsive, i. e., aged INS-1E cells. These results suggest that fatty acid deprivation (FFA-free BSA) impairs GSIS mainly through an effect on Ca2+sensitivity.

Background Obesity, metabolic syndrome (MetS), and diabetes are frequent in surgical populations and can enhance susceptibility to postoperative surgical site infections. Reduced neutrophil function has been linked with diabetes and risk of Staphylococcus aureus infection. Therefore, neutrophil function in diabetic and obese subjects (± MetS) was assessed in this prospective serological and cellular surveillance study to determine whether vaccines administered to protect against infections after surgery could be effective in these populations. Methods Neutrophil function (chemotaxis, phagocytosis, and opsonophagocytic killing of S. aureus) was assessed in subjects classified according to diabetes status, body mass index, and presence/absence of MetS. Neutrophils were characterized within functional subsets by flow cytometry. A serologic assay was used to measure baseline antibody presence to each antigen in SA4Ag: capsular polysaccharide (CP) type 5, CP8, recombinant mutant Clumping factor A (rmClfA), and recombinant Manganese transport protein C (rMntC). Results Neutrophil function was similar for comorbid and healthy cohorts, with no significant between-group differences in cell counts, migration, phagocytosis ability, neutrophil subset proportions, and S. aureus killing ability when neutrophils were isolated 3–6 months apart (Visit 1 [n = 90] and Visit 2 [n = 70]) and assessed. Median pre-existing antibody titers to CP5, CP8, and rmClfA were comparable for all cohorts (insufficient subjects with rMntC titers for determination). Conclusions MetS, diabetes, and obesity do not impact in vitro neutrophil function with regard to S. aureus killing, suggesting that if an effective S. aureus vaccine is developed it may be effective in individuals with these comorbidities.

Multiple myeloma (MM) is a hematological cancer with underlying causes associated with increased oxidative stress. Through signaling of their receptor RAGE, advanced glycation endproducts (AGEs) are known to increase oxidative stress associated with malignant transformation. In the present study, we have demonstrated that the levels of these compounds are increased in the saliva of myeloma patients with bone lesions. This data may provide a potential marker for bone lesions in MM and a potential target for the treatment of myeloma by blocking the AGEs or their receptor.

BACKGROUND: Conventional colonoscopy (CC) is the gold standard for diagnostic examination of the colon. However, the overall acceptance of this procedure is low due to patient fears of complications or embarrassment. Colon capsule endoscopy (CCE) represents a minimally invasive, patient-friendly procedure that offers complete visualization of the entire intestine.OBJECTIVE: To assess the PillCam Colon 2 (Given Imaging Ltd, Israel) capsule with regard to feasibility, sensitivity and specificity for the detection of colonic pathologies and additional recorded extracolonic findings.METHODS: CCE was performed before CC in patients indicated for CC for known or suspected colonic disease. The results of both techniques were compared with regard to polyp detection. Additionally, bowel preparation and extracolonic pathologies were analyzed.RESULTS: Twenty-four patients (mean age 51.1 years) were included in the analysis. Visualization of the colon was complete in 23 CCs and 17 CCEs. No adverse events or major technical failures occurred. CC detected 47 polyps and CCE detected 43 polyps of any size (per-finding sensitivity 90.9%, specificity 67.6%). The accuracy of CCE in detecting polyp carriers was 81.5% (per-patient analysis). On average, the colon was adequately cleansed in 90.1% of patients. CCE identified esophageal, gastric and small bowel pathologies in seven (24%), nine (38%) and 14 (58%) patients, respectively.CONCLUSIONS: CCE proved to be technically feasible and safe. Acceptable sensitivity and moderate specificity levels in polyp detection were recorded. Bowel preparation was adequate in most patients. Because extracolonic pathologies were effectively visualized, new indications for the PillCam Colon 2 may be defined.

Staphylococci cause diseases ranging from relatively mild superficial skin infections to life-threatening conditions, including sepsis and endocarditis. Increasingly, staphylococci have developed antibiotic resistance, reducing treatment options and highlighting the need for an effective prophylactic vaccine. Preclinical studies have assessed several antigens that, either alone [1, 2] or in combination [3], have the ability to reduce staphylococcal disease in preclinical models. Staphylococcal strains are known for the phenotypic plasticity of their antigenic repertoire, which provides mechanisms for both survival in diverse host niches and for immune evasion. Thus the development of a broadly protective vaccine that can prevent different diseases caused by diverse strains from within the species group poses challenges. S. aureus distinguishes itself from other staphylococci by an impressive array of virulence factors [4] and the production of coagulase. S. epidermidis also causes disease with associated mortality [5, 6]. S. aureus and S. epidermidis share a core genome representing approximatly two thirds of their genes; the proteins encoded by the core genesaverage 70% amino acid sequence identity between the two organisms [4]. We were interested in evaluating proteins with the potential to prevent staphylococcal disease caused by either S. aureus or S. epidermidis. Effective bacterial vaccine components should be conserved and perform important functions during infection. Initial attempts to identify S. aureus vaccine candidates came from assessment of surface antigens from bacteria grown in vitro [7]. Advances in in vivo technology have led to a better understanding of bacterial antigen expression during infection, enhancing our knowledge of host-pathogen relationships. When S. aureus and other pathogenic bacteria invade, they initiate expression of multiple virulence pathways. S. aureus produces capsular polysaccharide to avoid attack from the innate immune system. It also expresses various ligand-binding proteins with roles ranging from immune cloaking [8] to scavenging essential ions [9]. One group of proteins associated with in vivo survival of S. aureus are the manganese transport (Mnt) proteins. The Mnt complex is an ABC transporter composed of an ATP-binding protein (MntA), an integral membrane transporter (MntB), and a manganese binding surface lipoprotein (MntC) [10]. Recently MntC was identified as being expressed on the cell surface of S. aureus in biofilms generated in in vivo models of infection [11]. The orthologous protein in S. epidermidis is staphylococcal iron transport C (SitC) [12], which was detected in convalescent-phase serum from rabbits infected with S. epidermidis and found to be protective in a S. epidermidis murine kidney abscess model [13]. A critical attribute for an effective staphylococcal vaccine antigen is early expression during infection, which provides an opportunity for vaccine-induced antibodies to inactivate bacteria before infection is firmly established. We therefore evaluated S. aureus MntC temporal expression in vivo. S. aureus MntC and S. epidermidis SitC have a high level of sequence identity (72%), and polyclonal antisera have been demonstrated to be cross-reactive [13]. We explored whether S. aureus MntC could produce cross-protective antibodies against both S. aureus and S. epidermidis infection.