1. More than just inflammation: Ureaplasma species induce apoptosis in human brain microvascular endothelial cells
- Author
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Christine Silwedel, Axel Haarmann, Markus Fehrholz, Heike Claus, Christian P. Speer, and Kirsten Glaser
- Subjects
Ureaplasma urealyticum ,Ureaplasma parvum ,Neuroinflammation ,Meningitis ,Caspase ,Apoptosis ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Ureaplasma species (spp.) are commonly regarded as low-virulent commensals but may cause invasive diseases in immunocompromised adults and in neonates, including neonatal meningitis. The interactions of Ureaplasma spp. with host defense mechanisms are poorly understood. This study addressed Ureaplasma-driven cell death, concentrating on apoptosis as well as inflammatory cell death. Methods Human brain microvascular endothelial cells (HBMEC) were exposed to Ureaplasma (U.) urealyticum serovar 8 (Uu8) and U. parvum serovar 3 (Up3). Resulting numbers of dead cells as well as mRNA levels and enzyme activity of key agents in programmed cell death were assessed by flow cytometry, RNA sequencing, and qRT-PCR, respectively. xCELLigence data were used for real-time monitoring of changes in cell adhesion properties. Results Both Ureaplasma isolates induced cell death (p
- Published
- 2019
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