14 results on '"Christine Jerôme"'
Search Results
2. Contributors
- Author
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Banafshe Abadi, Nor Hasmaliana binti Abdul Manas, Raquel Silva Araújo, Sayang Baba, Parsa Bazdar, Thomas Beuvier, Muhammad Bilal, Parameswaran Binod, Frank Boury, Nela Buchtová, Juliana Cancino-Bernardi, Valéria Maria de Oliveira Cardoso, Guillermo R. Castro, Zafer Ceylan, Rakshita Chaudhary, Edson José Comparetti, Thomas Cordonnier, María L. Cuestas, Maria Alice de Oliveira, Daniel Joe Dailin, Tomás Brito Devoto, Batul Diwan, Mustafa Durmus, Touba Eslaminejad, João Paulo Fabi, Leonardo Miziara Barboza Ferreira, Natália Ferreira (Noronha), Moreno Galleni, Emmanuel Garcion, Nisha Gaur, Alain Gibaud, Ram K. Gupta, Salehhuddin Hamdan, Zanariah Hashim, Leila N. Hassani, François Hindre, Fitrien Husin, Jayanant Iemsam-arng, Rosli Md. Illias, Christine Jerôme, Norsuhada Abdul Karim, Koray Korkmaz, María F. Ladetto, María J. Limeres, Marina Guimarães Carvalho Machado, Samuel L. Martins, Siti Alyani Binti Mat, Gincy Marina Mathew, Aizi Nor Mazila Ramli, Renata Rank Miranda, Vishal Mishra, Vanessa Carla Furtado Mosqueira, Ida Idayu Muhamad, Mahshid Moballegh Nasery, Hoang Lam Nguyen, Tuan Anh Nguyen, Nor Farahiyah Aman Nor, Ashok Pandey, Sarva Mangala Praveena, Reshmy R, Bathabile Ramalapa, Maria H. Ribeiro, Thiécla Katiane Osvaldt Rosales, Nasrin Seyedpour, Shalyda Shaarani, Raveendran Sindhu, Mohan P Singh, Nidhi Singh, Veer Singh, Deepa Thomas, María A. Toscanini, Yilmaz Ucar, Manisha Verma, Manoj Kumar Verma, Nur Izyan Wan Azelee, Harisun Yaakob, Binti Abang Zaidel, Dayang Norulfairuz Abang Zaidel, Noorazwani Zainol, Siti Fatimah Zaharah Mohd Fuzi, and Valtencir Zucolotto
- Published
- 2022
3. Albumin-Based Hydrogel Films Covalently Cross-Linked with Oxidized Gellan with Encapsulated Curcumin for Biomedical Applications
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Camelia Elena Tincu (Iurciuc), Oana Maria Daraba, Christine Jérôme, Marcel Popa, and Lăcrămioara Ochiuz
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albumin-based hydrogel ,oxidized gellan ,cross-linking of bovine serum albumin ,biopolymers ,Organic chemistry ,QD241-441 - Abstract
Bovine serum albumin (BSA) hydrogels are non-immunogenic, low-cost, biocompatible, and biodegradable. In order to avoid toxic cross-linking agents, gellan was oxidized with NaIO4 to obtain new functional groups like dialdehydes for protein-based hydrogel cross-linking. The formed dialdehyde groups were highlighted with FT-IR and NMR spectroscopy. This paper aims to investigate hydrogel films for biomedical applications obtained by cross-linking BSA with oxidized gellan (OxG) containing immobilized β-cyclodextrin–curcumin inclusion complex (β-CD–Curc) The β-CD–Curc improved the bioavailability and solubility of Curc and was prepared at a molar ratio of 2:1. The film’s structure and morphology were evaluated using FT-IR spectroscopy and SEM. The swelling degree (Q%) values of hydrogel films depend on hydrophilicity and pH, with higher values at pH = 7.4. Additionally, the conversion index of -NH2 groups into Schiff bases increases with an increase in OxG amount. The polymeric matrix provides protection for Curc, is non-cytotoxic, and enhances antioxidant activity. At pH = 5.5, the skin permeability and release efficiency of encapsulated curcumin were higher than at pH = 7.4 because of the interaction of free aldehyde and carboxylic groups from hydrogels with amine groups from proteins present in the skin membrane, resulting in a better film adhesion and more efficient curcumin release.
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- 2024
- Full Text
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4. Controlled Radical Polymerization: Materials
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Krzysztof Matyjaszewski, Brent S. Sumerlin, Nicolay V. Tsarevsky, John Chiefari, Kotaro Satoh, Daisuke Ito, Masami Kamigaito, Yi Yan, Jiuyang Zhang, Chuanbing Tang, Athina Anastasaki, Vasiliki Nikolaou, Fehaid Alsubaie, Alexandre Simula, Christopher Waldron, Danielle J. Lloyd, Jamie Godfrey, Gabit Nurumbetov, Qiang Zhang, Paul Wilson, Kristian Kempe, David M. Haddleton, Jérémy Demarteau, Anthony Kermagoret, Christine Jérôme, Christophe Detrembleur, Antoine Debuigne, Lenny Voorhaar, Kristof Van Hecke, Gertjan Vancoillie, Qilu Zhang, Richard Hoogenboom, Zhongfan Jia, Michael J. Monteiro, Jiacheng Zhao, Martina H. Stenzel, Fenja Moldenhauer, Patrick Theato, Jun Ling, Axel H. E. Müller, Yi Shi, Robert W. Graff, Haifeng Gao, Shannon R. Woodruff, Peng-Fei Cao, Edward Foster, Al de Leon, Rigoberto Advincula, Lei Wang, Junting Li, Yang Zheng, Yucheng Huang, Yali Qiao, Brian C. Benicewicz, Rinaldo Poli, Si Chen, Xuewei Zhang, Andrés Cardozo, Muriel Lansalot, Franck D’Agosto, Bernadette Charleux, Eric Manoury, Florence Gayet, Carine Julcour, Jean-François Bl, Krzysztof Matyjaszewski, Brent S. Sumerlin, Nicolay V. Tsarevsky, John Chiefari, Kotaro Satoh, Daisuke Ito, Masami Kamigaito, Yi Yan, Jiuyang Zhang, Chuanbing Tang, Athina Anastasaki, Vasiliki Nikolaou, Fehaid Alsubaie, Alexandre Simula, Christopher Waldron, Danielle J. Lloyd, Jamie Godfrey, Gabit Nurumbetov, Qiang Zhang, Paul Wilson, Kristian Kempe, David M. Haddleton, Jérémy Demarteau, Anthony Kermagoret, Christine Jérôme, Christophe Detrembleur, Antoine Debuigne, Lenny Voorhaar, Kristof Van Hecke, Gertjan Vancoillie, Qilu Zhang, Richard Hoogenboom, Zhongfan Jia, Michael J. Monteiro, Jiacheng Zhao, Martina H. Stenzel, Fenja Moldenhauer, Patrick Theato, Jun Ling, Axel H. E. Müller, Yi Shi, Robert W. Graff, Haifeng Gao, Shannon R. Woodruff, Peng-Fei Cao, Edward Foster, Al de Leon, Rigoberto Advincula, Lei Wang, Junting Li, Yang Zheng, Yucheng Huang, Yali Qiao, Brian C. Benicewicz, Rinaldo Poli, Si Chen, Xuewei Zhang, Andrés Cardozo, Muriel Lansalot, Franck D’Agosto, Bernadette Charleux, Eric Manoury, Florence Gayet, Carine Julcour, and Jean-François Bl
- Subjects
- Free radical reactions, Polymerization
- Published
- 2015
5. Progress in Controlled Radical Polymerization: Mechanisms and Techniques
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Krzysztof Matyjaszewski, Brent Sumerlin, Nicolay V. Tsarevsky, Isa Degirmenci, Benjamin B. Noble, Ching Yeh Lin, Michelle L. Coote, Atsushi Kajiwara, Lebohang Hlalele, Bert Klumperman, Mehmet Atilla Tasdelen, Mustafa Çiftci, Mustafa Uygun, Yusuf Yagci, Carolynne L. Ricardo, Tomislav Pintauer, Shannon R. Woodruff, Brad J. Davis, Yannick Borguet, Lionel Delaude, Albert Demonceau, Kotaro Satoh, Tomohiro Abe, Masami Kamigaito, Dominik Konkolewicz, Dagmar R. D’hooge, Stanislaw Sosnowski, Ryszard Szymanski, Marie-Françoise Reyniers, Guy B. Marin, Gergely Kali, Tilana B. Silva, Severin J. Sigg, Farzad Seidi, Kasper Renggli, Nico Bruns, Kevin A. Payne, Michael F. Cunningham, Robin A. Hutchinson, Wojciech Jakubowski, Antoine Debuigne, Marie Hurtgen, Christine Jérôme, Christophe Detrembleur, Zhigang Xue, Rinaldo Poli, Graeme Moad, Erika Bicciocchi, Ming Chen, John Chiefari, Carlos Guerrero-Sanchez, Matthias Haeussler, Shadi Houshyar, Daniel Keddie, Ezio Rizzardo, San H. Thang, John Tsanaktsidis, Mathias Destarac, Issam Blidi, Olivier Coutelier, Aymeric G, Krzysztof Matyjaszewski, Brent Sumerlin, Nicolay V. Tsarevsky, Isa Degirmenci, Benjamin B. Noble, Ching Yeh Lin, Michelle L. Coote, Atsushi Kajiwara, Lebohang Hlalele, Bert Klumperman, Mehmet Atilla Tasdelen, Mustafa Çiftci, Mustafa Uygun, Yusuf Yagci, Carolynne L. Ricardo, Tomislav Pintauer, Shannon R. Woodruff, Brad J. Davis, Yannick Borguet, Lionel Delaude, Albert Demonceau, Kotaro Satoh, Tomohiro Abe, Masami Kamigaito, Dominik Konkolewicz, Dagmar R. D’hooge, Stanislaw Sosnowski, Ryszard Szymanski, Marie-Françoise Reyniers, Guy B. Marin, Gergely Kali, Tilana B. Silva, Severin J. Sigg, Farzad Seidi, Kasper Renggli, Nico Bruns, Kevin A. Payne, Michael F. Cunningham, Robin A. Hutchinson, Wojciech Jakubowski, Antoine Debuigne, Marie Hurtgen, Christine Jérôme, Christophe Detrembleur, Zhigang Xue, Rinaldo Poli, Graeme Moad, Erika Bicciocchi, Ming Chen, John Chiefari, Carlos Guerrero-Sanchez, Matthias Haeussler, Shadi Houshyar, Daniel Keddie, Ezio Rizzardo, San H. Thang, John Tsanaktsidis, Mathias Destarac, Issam Blidi, Olivier Coutelier, and Aymeric G
- Subjects
- Addition polymerization, Radicals (Chemistry)
- Published
- 2012
6. Conversion of Electrospun Chitosan into Chitin: A Robust Strategy to Tune the Properties of 2D Biomimetic Nanofiber Scaffolds
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Natalia Toncheva-Moncheva, Abdelhafid Aqil, Moreno Galleni, and Christine Jérôme
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chitosan ,chitin ,fiber ,electrospinning ,2D scaffold ,Biochemistry ,QD415-436 - Abstract
New biomimetic micro- and nano-CsU-based fibrous scaffolds electrospun from solution containing high purity-medical grade chitosan (CsU) of fungus origin (CsU1, Mv ~174,000 and CsU2, 205,000, degree of deacetylation (DDA) ~65%) and polyethylene oxide (PEO, Mv ~ 900,000), in the presence of given amounts of Triton X-100 (from 0.01 to 0.5 wt%) as surfactant were fabricated. We demonstrate that by carefully selecting compositions and surfactant levels, porous mats with CsU content up to 90% (at this molecular weight and DDA) were achieved. Remarkable long-term stability in water or phosphate buffer solution storage were obtained by developing post-electrospinning treatment allowing the complete elimination of the PEO from the CsU-fibers as demonstrated by TGA, DSC and ESEM analysis. Subsequent reacetylation procedure was applied to convert 2D biomimetic chitosan mats to chitin (CsE)-based ones while preserving the nanofiber structure. This innovative procedure allows tuning and modifying the thermal, mechanical properties and more importantly the biodegradation abilities (fast enzymatic biodegradation in some cases and slower on the others) of the prepared nanofibrous mats. The established reproducible method offers the unique advantage to modulate the membrane properties leading to stable 2D biomimetic CsU and/or chitin (CsE) scaffolds tailor-made for specific purposes in the field of tissue engineering.
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- 2021
- Full Text
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7. Properties and role of interfaces in multimaterial 3D printed composites
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Laura Zorzetto, Luca Andena, Francesco Briatico-Vangosa, Lorenzo De Noni, Jean-Michel Thomassin, Christine Jérôme, Quentin Grossman, Anne Mertens, Richard Weinkamer, Marta Rink, and Davide Ruffoni
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Medicine ,Science - Abstract
Abstract In polyjet printing photopolymer droplets are deposited on a build tray, leveled off by a roller and cured by UV light. This technique is attractive to fabricate heterogeneous architectures combining compliant and stiff constituents. Considering the layer-by-layer nature, interfaces between different photopolymers can be formed either before or after UV curing. We analyzed the properties of interfaces in 3D printed composites combining experiments with computer simulations. To investigate photopolymer blending, we characterized the mechanical properties of the so-called digital materials, obtained by mixing compliant and stiff voxels according to different volume fractions. We then used nanoindentation to measure the spatial variation in mechanical properties across bimaterial interfaces at the micrometer level. Finally, to characterize the impact of finite-size interfaces, we fabricated and tested composites having compliant and stiff layers alternating along different directions. We found that interfaces formed by deposition after curing were sharp whereas those formed before curing showed blending of the two materials over a length scale bigger than individual droplet size. We found structural and functional differences of the layered composites depending on the printing orientation and corresponding interface characteristics, which influenced deformation mechanisms. With the wide dissemination of 3D printing techniques, our results should be considered in the development of architectured materials with tailored interfaces between building blocks.
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- 2020
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8. Controlled/Living Radical Polymerization: Progress in RAFT, DT, NMP & OMRP
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Krzysztof Matyjaszewski, Graeme Moad, Y.K. Chong, Roger Mulder, Ezio Rizzardo, San H. Thang, Geoffrey Johnston-Hall, Michael J. Monteiro, Vidyasagar Malepu, Christy D. Petruczok, TuTrinh Tran, Tianxi Zhang, Mahesh Thopasridharan, Devon A. Shipp, Harumi Murayama, Kotaro Satoh, Masami Kamigaito, Jeff Tonnar, Patrick Lacroix-Desmazes, Anna C. Greene, Robert B. Grubbs, Dmitry F. Grishin, Elena V. Kolyakina, Marina V. Pavlovskaya, Mikhail A. Lazarev, Alexander A. Shchepalov, Chi-How Peng, Shan Li, Bradford B. Wayland, Antoine Debuigne, Rinaldo Poli, Robert Jérôme, Christine Jérôme, Christophe Detrembleur, Alexandru D. Asandei, Christopher P. Simpson, Hyun S. Yu, Olumide I. Adebolu, Gobinda Saha, Yanhui Chen, Gwenaelle Pound-Lana, Bert Klumperman, Qiang Yu, Qian Gan, Hongwen Zhang, Shiping Zhu, Matthew G. Kellum, Adam E. Smith, Xuewei Xu, Charles L. McCormick, D. Boschmann, M. Mänz, M. G. Fröhlich, G. Zifferer, P. Vana, Muhammad Imran-ul-haq, Nadja Förster, Radovan Vukicevic, Kristin Herrmann, Rebekka Siegmann, Sabine Beuermann, Didier Gigmes, Jerôme V, Krzysztof Matyjaszewski, Graeme Moad, Y.K. Chong, Roger Mulder, Ezio Rizzardo, San H. Thang, Geoffrey Johnston-Hall, Michael J. Monteiro, Vidyasagar Malepu, Christy D. Petruczok, TuTrinh Tran, Tianxi Zhang, Mahesh Thopasridharan, Devon A. Shipp, Harumi Murayama, Kotaro Satoh, Masami Kamigaito, Jeff Tonnar, Patrick Lacroix-Desmazes, Anna C. Greene, Robert B. Grubbs, Dmitry F. Grishin, Elena V. Kolyakina, Marina V. Pavlovskaya, Mikhail A. Lazarev, Alexander A. Shchepalov, Chi-How Peng, Shan Li, Bradford B. Wayland, Antoine Debuigne, Rinaldo Poli, Robert Jérôme, Christine Jérôme, Christophe Detrembleur, Alexandru D. Asandei, Christopher P. Simpson, Hyun S. Yu, Olumide I. Adebolu, Gobinda Saha, Yanhui Chen, Gwenaelle Pound-Lana, Bert Klumperman, Qiang Yu, Qian Gan, Hongwen Zhang, Shiping Zhu, Matthew G. Kellum, Adam E. Smith, Xuewei Xu, Charles L. McCormick, D. Boschmann, M. Mänz, M. G. Fröhlich, G. Zifferer, P. Vana, Muhammad Imran-ul-haq, Nadja Förster, Radovan Vukicevic, Kristin Herrmann, Rebekka Siegmann, Sabine Beuermann, Didier Gigmes, and Jerôme V
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- Polymerization--Congresses, Free radical reactions--Congresses
- Published
- 2009
9. Injectable Hydrogel Membrane for Guided Bone Regeneration
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Pauline Marie Chichiricco, Pietro Matricardi, Bruno Colaço, Pedro Gomes, Christine Jérôme, Julie Lesoeur, Joëlle Veziers, Gildas Réthoré, Pierre Weiss, Xavier Struillou, and Catherine Le Visage
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photo-crosslinking ,visible light photopolymerization ,silanized hydroxypropyl methylcellulose ,dextran methacrylate ,riboflavin ,calvaria bone regeneration ,Technology ,Biology (General) ,QH301-705.5 - Abstract
In recent years, multicomponent hydrogels such as interpenetrating polymer networks (IPNs) have emerged as innovative biomaterials due to the synergistic combination of the properties of each network. We hypothesized that an innovative non-animal IPN hydrogel combining self-setting silanized hydroxypropyl methylcellulose (Si-HPMC) with photochemically cross-linkable dextran methacrylate (DexMA) could be a valid alternative to porcine collagen membranes in guided bone regeneration. Calvaria critical-size defects in rabbits were filled with synthetic biphasic calcium phosphate granules in conjunction with Si-HPMC; DexMA; or Si-HPMC/DexMA experimental membranes; and in a control group with a porcine collagen membrane. The synergistic effect obtained by interpenetration of the two polymer networks improved the physicochemical properties, and the gel point under visible light was reached instantaneously. Neutral red staining of murine L929 fibroblasts confirmed the cytocompatibility of the IPN. At 8 weeks, the photo-crosslinked membranes induced a similar degree of mineral deposition in the calvaria defects compared to the positive control, with 30.5 ± 5.2% for the IPN and 34.3 ± 8.2% for the collagen membrane. The barrier effect appeared to be similar in the IPN test group compared with the collagen membrane. In conclusion, this novel, easy-to-handle and apply, photochemically cross-linkable IPN hydrogel is an excellent non-animal alternative to porcine collagen membrane in guided bone regeneration procedures.
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- 2023
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10. En Route to CO2-Based (a)Cyclic Carbonates and Polycarbonates from Alcohols Substrates by Direct and Indirect Approaches
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Antoine Brege, Bruno Grignard, Raphaël Méreau, Christophe Detrembleur, Christine Jerome, and Thierry Tassaing
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carbon dioxide ,alcohols ,(a)cyclic carbonates ,polycarbonates ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
This review is dedicated to the state-of-the art routes used for the synthesis of CO2-based (a)cyclic carbonates and polycarbonates from alcohol substrates, with an emphasis on their respective main advantages and limitations. The first section reviews the synthesis of organic carbonates such as dialkyl carbonates or cyclic carbonates from the carbonation of alcohols. Many different synthetic strategies have been reported (dehydrative condensation, the alkylation route, the “leaving group” strategy, the carbodiimide route, the protected alcohols route, etc.) with various substrates (mono-alcohols, diols, allyl alcohols, halohydrins, propargylic alcohols, etc.). The second section reviews the formation of polycarbonates via the direct copolymerization of CO2 with diols, as well as the ring-opening polymerization route. Finally, polycondensation processes involving CO2-based dimethyl and diphenyl carbonates with aliphatic and aromatic diols are described.
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- 2022
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11. Polysaccharides-Based Complex Particles’ Protective Role on the Stability and Bioactivity of Immobilized Curcumin
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Camelia-Elena Iurciuc (Tincu), Leonard Ionuţ Atanase, Christine Jérôme, Vincent Sol, Patrick Martin, Marcel Popa, and Lăcrămioara Ochiuz
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polysaccharides particles ,polymeric matrix ,immobilized curcumin ,pH and light stability ,curcumin metal complexes ,antioxidant activity ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The curcumin degradation represents a significant limitation for its applications. The stability of free curcumin (FC) and immobilized curcumin in complex particles (ComPs) based on different polysaccharides was studied under the action of several factors. Ultraviolet-visible (UV-VIS) and Fourier-transform infrared (FTIR) spectroscopy proved the FC photodegradation and its role as a metal chelator: 82% of FC and between 26% and 39.79% of curcumin within the ComPs degraded after exposure for 28 days to natural light. The degradation half-life (t1/2) decreases for FC when the pH increases, from 6.8 h at pH = 3 to 2.1 h at pH = 9. For curcumin extracted from ComPs, t1/2 was constant (between 10 and 13 h) and depended on the sample’s composition. The total phenol (TPC) and total flavonoids (TFC) content values increased by 16% and 13%, respectively, for FC exposed to ultraviolet light at λ = 365 nm (UVA), whereas no significant change was observed for immobilized curcumin. Antioxidant activity expressed by IC50 (µmoles/mL) for FC exposed to UVA decreased by 29%, but curcumin within ComPs was not affected by the UVA. The bovine serum albumin (BSA) adsorption efficiency on the ComPs surface depends on the pH value and the cross-linking degree. ComPs have a protective role for the immobilized curcumin.
- Published
- 2021
- Full Text
- View/download PDF
12. Thiol-ene Reaction: An Efficient Tool to Design Lipophilic Polyphosphoesters for Drug Delivery Systems
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Stéphanie Vanslambrouck, Raphaël Riva, Bernard Ucakar, Véronique Préat, Mick Gagliardi, Daniel G. M. Molin, Philippe Lecomte, and Christine Jérôme
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click chemistry ,biodegradable polymer ,polyphosphoester ,drug-delivery ,Organic chemistry ,QD241-441 - Abstract
Poly(ethylene glycol)-b-polyphosphoester (PEG-b-PPE) block copolymer nanoparticles are promising carriers for poorly water soluble drugs. To enhance the drug loading capacity and efficiency of such micelles, a strategy was investigated for increasing the lipophilicity of the PPE block of these PEG-b-PPE amphiphilic copolymers. A PEG-b-PPE copolymer bearing pendant vinyl groups along the PPE block was synthesized and then modified by thiol-ene click reaction with thiols bearing either a long linear alkyl chain (dodecyl) or a tocopherol moiety. Ketoconazole was used as model for hydrophobic drugs. Comparison of the drug loading with PEG-b-PPE bearing shorter pendant groups is reported evidencing the key role of the structure of the pendant group on the PPE backbone. Finally, a first evidence of the biocompatibility of these novel PEG-b-PPE copolymers was achieved by performing cytotoxicity tests. The PEG-b-PPE derived by tocopherol was evidenced as particularly promising as delivery system of poorly water-soluble drugs.
- Published
- 2021
- Full Text
- View/download PDF
13. Curdlan–Chitosan Electrospun Fibers as Potential Scaffolds for Bone Regeneration
- Author
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Clément Toullec, Jean Le Bideau, Valerie Geoffroy, Boris Halgand, Nela Buchtova, Rodolfo Molina-Peña, Emmanuel Garcion, Sylvie Avril, Laurence Sindji, Admire Dube, Frank Boury, and Christine Jérôme
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curdlan ,chitosan ,electrospinning ,regenerative medicine ,tissue engineering ,Organic chemistry ,QD241-441 - Abstract
Polysaccharides have received a lot of attention in biomedical research for their high potential as scaffolds owing to their unique biological properties. Fibrillar scaffolds made of chitosan demonstrated high promise in tissue engineering, especially for skin. As far as bone regeneration is concerned, curdlan (1,3-β-glucan) is particularly interesting as it enhances bone growth by helping mesenchymal stem cell adhesion, by favoring their differentiation into osteoblasts and by limiting the osteoclastic activity. Therefore, we aim to combine both chitosan and curdlan polysaccharides in a new scaffold for bone regeneration. For that purpose, curdlan was electrospun as a blend with chitosan into a fibrillar scaffold. We show that this novel scaffold is biodegradable (8% at two weeks), exhibits a good swelling behavior (350%) and is non-cytotoxic in vitro. In addition, the benefit of incorporating curdlan in the scaffold was demonstrated in a scratch assay that evidences the ability of curdlan to express its immunomodulatory properties by enhancing cell migration. Thus, these innovative electrospun curdlan–chitosan scaffolds show great potential for bone tissue engineering.
- Published
- 2021
- Full Text
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14. In vitro evaluation of biocompatibility of uncoated thermally reduced graphene and carbon nanotube-loaded PVDF membranes with adult neural stem cell-derived neurons and glia
- Author
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Çagla Defterali, Raquel Verdejo, Shahid Majeed, Adriana Boschetti-de-Fierro, Héctor R Méndez-Gómez, Eva Díaz-Guerra, Daniel Fierro, Kristian Buhr, Clarissa Abetz, Ricardo Martinez-Murillo, Daniela Vuluga, Michäel Alexandre, Jean-Michel Thomassin, Christophe Detrembleur, Christine Jérôme, Volker Abetz, Miguel Ángel López-Manchado, and CARLOS Vicario-Abejón
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Synapses ,Carbon nanotubes ,PVDF membranes ,Uncoated graphene ,Neurons ,oligodendrocytes ,Biotechnology ,TP248.13-248.65 - Abstract
Graphene, graphene-based nanomaterials (GBNs) and carbon nanotubes (CNTs) are being investigated as potential substrates for the growth of neural cells. However, in most in vitro studies the cells were seeded on these materials coated with various proteins implying that the observed effects on the cells could not solely be attributed to the GBN and CNT properties. Here we studied the biocompatibility of uncoated thermally reduced graphene (TRG) and poly-vinylidene fluoride (PVDF) membranes loaded with multi walled CNTs (MWCNTs) using neural stem cells (NSCs) isolated from the adult mouse olfactory bulb (termed aOBSCs). When aOBSCs were induced to differentiate on coverslips treated with TRG or control materials (polyethyleneimine-PEI and polyornithine plus fibronectin-PLO/F) in a serum-free medium, neurons, astrocytes, and oligodendrocytes were generated in all conditions, indicating that TRG permits the multi-lineage differentiation of aOBSCs. However, the total number of cells was reduced on both PEI and TRG. In a serum-containing medium, aOBSC-derived neurons and oligodendrocytes grown on TRG were more numerous than in controls; the neurons developed synaptic boutons and oligodendrocytes were more branched. In contrast, neurons growing on PVDF membranes had reduced neurite branching and on MWCNTs-loaded membranes, oligodendrocytes were lower in numbers than in controls. Overall, these findings indicate that uncoated TRG may be biocompatible with the generation, differentiation, and maturation of aOBSC-derived neurons and glial cells, implying a potential use for TRG to study functional neuronal networks.
- Published
- 2016
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- View/download PDF
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