1. Interactions of elongation factor EF-P with the Escherichia coli ribosome.
- Author
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Aoki H, Xu J, Emili A, Chosay JG, Golshani A, and Ganoza MC
- Subjects
- Binding Sites, Immunoprecipitation, Mass Spectrometry, Protein Binding, RNA, Ribosomal, 16S metabolism, RNA, Ribosomal, 23S metabolism, Escherichia coli metabolism, Peptide Elongation Factors metabolism, Ribosomes metabolism
- Abstract
EF-P (eubacterial elongation factor P) is a highly conserved protein essential for protein synthesis. We report that EF-P protects 16S rRNA near the G526 streptomycin and the S12 and mRNA binding sites (30S T-site). EF-P also protects domain V of the 23S rRNA proximal to the A-site (50S T-site) and more strongly the A-site of 70S ribosomes. We suggest that EF-P: (a) may play a role in translational fidelity and (b) prevents entry of fMet-tRNA into the A-site enabling it to bind to the 50S P-site. We also report that EF-P promotes a ribosome-dependent accommodation of fMet-tRNA into the 70S P-site.
- Published
- 2008
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