1. Changes in Relative Counts of Different Leukocyte Subpopulations in Peripheral and Umbilical Cord Blood of Women With Preterm Prelabor Rupture of Membranes With Respect to Intraamniotic Inflammation and Fetal Inflammatory Response Syndrome.
- Author
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Soucek O, Kacerovsky M, Musilova IK, Stranik J, Kukla R, Bolehovska R, Hornychova H, and Andrys C
- Subjects
- Humans, Female, Pregnancy, Adult, Leukocyte Count, Amniotic Fluid immunology, Amniotic Fluid metabolism, Inflammation immunology, Chorioamnionitis immunology, Chorioamnionitis blood, Systemic Inflammatory Response Syndrome immunology, Systemic Inflammatory Response Syndrome blood, Leukocytes immunology, Flow Cytometry, Interleukin-6 blood, Interleukin-6 metabolism, Fetal Blood immunology, Fetal Blood cytology, Fetal Membranes, Premature Rupture immunology, Fetal Membranes, Premature Rupture blood
- Abstract
Objective: The aim of this study was to evaluate changes in the relative counts of different leukocyte subsets in peripheral and umbilical cord blood in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) with respect to the presence of intraamniotic inflammation (IAI) and fetal inflammatory response syndrome (FIRS)., Methods: Fifty-two women with singleton pregnancies complicated by PPROM were included in this study. From samples of peripheral and umbilical cord blood, relative counts of these leukocyte subpopulations were determined using multicolor flow cytometry: granulocytes, monocytes, lymphocytes, T cells and their subpopulations, B cells and their subpopulations, and NK cells and their subpopulations. IAI was defined as increased concentrations of interleukin 6 in the amniotic fluid. Amniotic fluid samples were obtained by transabdominal amniocentesis., Results: Women with IAI had higher relative counts of monocytes (p = 0.04) in peripheral blood. There was an increased relative number of granulocytes (p = 0.003) and a decreased number of lymphocytes (p = 0.0048), helper CD4+ T cells (p = 0.019), NK cells (p = 0.0001) within leukocytes, NK cells within lymphocytes (p = 0.003) and CD16+ NK cells within NK cells (p = 0.005) in umbilical cord blood samples of women with FIRS. However, after adjusting the results for gestational age at sampling, all differences disappeared., Conclusions: The presence of IAI or FIRS is not accompanied by significant changes in the relative counts of immune cells in peripheral blood or umbilical cord blood in pregnancies complicated by PPROM., (© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2024
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