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AbstractMeliosma oldhamii Miq. is a deciduous arbor and a member of Sabiaceae. It is one of the rare and protected plants with outstanding ornamental and economic value in Jiangsu, China. In addition to the resource values indicated above, there are numerous other elements that require additional research, including the chloroplast (cp) genomic information. In this work, the complete cp genome sequence of M. oldhamii was assembled and characterized for the first time. The complete cp genome of M. oldhamii was 159,950 base pair (bp) in length, including a large single-copy (LSC) region of 87,147 bp, a small single-copy (SSC) region of 18,015 bp, and the inverted repeats (IRs) region of 27,394 bp. It contains 131 genes, including 37 tRNA genes, eight rRNA genes, 85 protein-coding genes, and one pseudogene. The overall GC content of M. oldhamii cp genome was 37.95%. The placement of M. oldhamii in the phylogenetic tree constructed using the complete cp genome is largely congruent with previous studies. The clustering of Meliosma is non-monophyletic, aligns more closely with existing morphological taxonomic studies, thereby enhancing the scholarly and scientific nature of this research.

Styrax serrulatus Roxburgh (William Roxburgh 1832), which plays an important role in ecology and economy, is a deciduous species of Styracaceae. In this paper, we sequenced, assembled, and annotated the chloroplast (cp) genome of S. serrulatus by using the sequencing data from Illumina Novaseq platform (Illumina, San Diego, CA). The complete cp genome of S. serrulatus is 157,929 base pairs (bp) in length, containing a pair of inverted repeat regions (IRs) of 26,048 bp each, a large single-copy (LSC) region of 87,552 bp, and a small single-copy (SSC) region of 18,281 bp. It contains 133 genes, including 8 rRNA genes, 37 tRNA genes, 87 protein-coding genes, and 1 pseudo gene. The GC content of S. serrulatus cp genome is 36.96%. The phylogenetic analysis suggests that S. serrulatus is a sister species to Styrax agrestis in Styracaceae.

Ehretia dicksonii_Hance 1862 is a deciduous botany of Ehretiaceae. In this work, the complete chloroplast (cp) genome sequence of E. dicksonii was probed by next generation sequencing in an effort to provide genomic resources useful for promoting its conservation. The complete cp genome of E. dicksonii is 156,623 bp in length, including a large single-copy (LSC) region of 86,853 bp, and a small single-copy (SSC) region of 18,150 bp. It contains 133 genes, including 37 tRNA genes, 8 rRNA genes, and 88 protein-coding genes. The overall GC content of E. dicksonii chloroplast genome is 37.85%. The phylogenetic analysis suggests that E. dicksonii is in the clade of Ehretiaceae other than Boraginaceae. Also, E. dicksonii has a close relationship with Ehretia acuminata_Brown 1810 in Ehretiaceae.

Ulmus lanceifolia, which plays an important role in ecology and economy, is one of the main evergreen broad leaved arbor species in southern China. In this study, we assembled its complete chloroplast genome. The total genome size of U. lanceifolia was 158,652bp, containing a large single-copy region of 87,119bp, a small single-copy region of 18,697bp, and a pair of inverted repeat regions of 26,418bp. The overall GC content of U. lanceifolia chloroplast genome is 35.63%. We obtained the 132 genes chloroplast genome, including 86 proteincoding genes, 8 rRNA genes, and 37 tRNA genes. The phylogenetic analysis suggests that U. lanceifolia is a monophyletic taxa and a transitional species between the 2 groups which were combined by Ulmus americana, U. elongate and Zelkova serrate, Z. schneideriana respectively in Ulmaceae.

Xylosma racemosa (Siebold & Zucc.) Miq. which plays an important role in ecology, medicine and economy, is a deciduous species of Salicaceae. In this paper, we sequenced, assembled, and annotated the chloroplast (cp) genome of X. racemosa (Siebold & Zucc.) Miq. by using the sequencing data from Illumina Novaseq platform. The complete cp genome of X. racemosa is 157,262 bp in length, containing a large single-copy (LSC) region of 84,289 bp, and a small single-copy (SSC) region of 17,817 bp. It contains 131 genes, including 8 rRNA genes, 37 tRNAs genes and 86 protein-coding genes. The GC content of X. racemosa cp genome is 36.74%. The phylogenetic analysis suggests that X. racemosa is a sister species to Xylosma congesta in Salicaceae.

Styrax tonkinensis Craib ex Hartwich is a deciduous species of Styracaceae family with beautiful shape, drooping flowers, blooming like snow. Here, we characterized the complete chloroplast (cp) genome of S. tonkinensis using next-generation sequencing method. The circular complete cp genome of S. tonkinensis is 157,934 bp in length, containing a large single-copy (LSC) region of 87,480 bp, and a small single-copy (SSC) region of 18,354 bp. It comprises 134 genes, including 8 rRNA genes, 37 tRNAs genes, and 89 protein-coding genes. The GC content of S. tonkinensis cp genome is 36.92%. The phylogenetic analysis suggests that S. tonkinensis is a sister species to S. obassia in Styracaceae.

Styrax obassia Siebold & Zucc. is a distinct member of the family Styracaceae with fragrant and beautiful flowers. In this study, we determined the complete chloroplast (cp) genome sequence of S. obassia in an effort to provide genomic resources useful for promoting its conservation. The circular genome of S. obassia was 157,910 bp in size and contained two inverted repeat (IRa and IRb) regions of 26,051 bp, which were separated by a large single copy (LSC) region of 87,528 bp, and a small single copy (SSC) region of 18,280 bp. A total of 133 genes are encoded, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall GC content of S. obassia genome is 36.97%. The phylogenetic analysis suggests that S. obassia is a sister species to Styrax suberifolius and Styrax zhejiangensis in Styracaceae.

Styrax calvescens Perk., is a nice tree, valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. calvescens using next-generation sequencing. The circular complete cp genome of S. calvescens is 157,951 bp in length, containing a large single-copy (LSC) region of 87,566 bp and a small single-copy (SSC) region of 18,289 bp. It comprises of 133 genes, including 8 rRNA genes, 37 tRNAs genes, and 88 protein-coding genes. The GC content of S. calvescens cp genome is 36.95%. The phylogenetic analysis suggests that S. calvescens is a sister species to Styrax grandiflorus Griffith in Styracaceae.

Styrax hemsleyanus is valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. hemsleyanus using next generation sequencing. The circular complete cp genome of S. hemsleyanus is 158,027 bp in length, containing a large single-copy (LSC) region of 87,641 bp, and a small single-copy (SSC) region of 18,290 bp. It comprises 133 genes, including 8 rRNA genes, 37 tRNAs genes, and 88 protein-coding genes. The GC content of S. hemsleyanus cp genome is 36.95%. The phylogenetic analysis suggests that S. hemsleyanus is a sister species to Styrax odoratissimus in Styracaceae.

Styrax confusus Hemsl. is valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. confusus using next-generation sequencing. The circular complete cp genome of S. confusus was 157,981 bp in length, containing a large single-copy (LSC) region of 87,571 bp, and a small single-copy (SSC) region of 18,316 bp. It comprises 133 genes, including eight rRNA genes, 37 tRNA genes, and 88 protein-coding genes. The GC content of S. confusus cp genome is 36.95%. A phylogenetic tree reconstructed by 30 chloroplast genomes reveals that S. confusus is mostly related to Styrax calvescens Perk.

In this work, next-generation sequencing was applied to measure the complete chloroplast genome of S. dasyanthus for the sake of offering valuable genomic information to promote its conservation. The complete chloroplast genome sequence of S. dasyanthus was measured as 157,501 bp in size. It contains the typical structure and gene content of angiosperm plastome, which includes a large single-copy (LSC), a small single-copy (SSC), and 2 inverted repeat (IR) regions of 87,130 bp, 18,277 bp, and 26,047 bp, respectively. The S. dasyanthus chloroplast genome has a total of 130 genes including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Overall, GC contents of the genome were 36.96%. Phylogenetic analysis based on the 29 chloroplast genome measurement demonstrated that S. dasyanthus is most closely related to Styrax grandiflours Griff.

The object of this work was to measure the complete chloroplast genome of Styrax duclouxii Perkins for the sake of offering valuable genomic information to promote its conservation. The circular genome of S. duclouxii was measured as 157,913 bp in size and contained two inverted repeat (IRa and IRb) regions of 26,040 bp, which were separated by a large single-copy (LSC) region of 87,604 bp and a small single copy (SSC) region of 18,229 bp. A total of 134 genes are encoded, including 89 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content of Schizosaccharomyces japonicus genome is 36.97%. A phylogenetic tree reconstructed using 36 chloroplast genomes reveals that S. duclouxii is most closely related with Styrax zhejiangensis and Styrax faberi.