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26 results on '"Chong, Nelson W."'

8. Melatonin synthesis in retina: cAMP-dependent transcriptional regulation of chicken arylalkylamine N-acetyltransferase (Aanat) by a CRE-like sequence (CLS) and a TTATT repeat motif in the proximal promoter

Author

Haque, Rashidul, Chong, Nelson W., Ali, Fatima, Chaurasia, Shyam S., Sengupta, Trisha, Chun, Eugene, Howell, Jennifer C., Klein, David C., and Iuvone, P. Michael

Subjects
Cell Nucleus, Chromatin Immunoprecipitation, 5' Flanking Region, Proto-Oncogene Proteins c-jun, Electrophoretic Mobility Shift Assay, Chick Embryo, Transfection, Arylalkylamine N-Acetyltransferase, Article, Gene Expression Regulation, Enzymologic, Retina, Cyclic AMP, Mutagenesis, Site-Directed, Animals, RNA, Small Interfering, Cyclic AMP Response Element-Binding Protein, Luciferases, Promoter Regions, Genetic, Proto-Oncogene Proteins c-fos, Cells, Cultured, DNA Primers, Melatonin, Repetitive Sequences, Nucleic Acid
Abstract

Arylalkylamine N-acetyltransferase (AANAT) is the key regulatory enzyme controlling the daily rhythm of melatonin biosynthesis. In chicken retinal photoreceptor cells, Aanat transcription and AANAT activity are regulated in part by cAMP-dependent mechanisms. The purpose of this study was to identify regulatory elements within the chicken Aanat promoter responsible for cAMP-dependent induction. Photoreceptor-enriched retinal cell cultures were transfected with a luciferase reporter construct containing up to 4 kb of 5'-flanking region and the first exon of Aanat. Forskolin treatment stimulated luciferase activity driven by the ∼4 kb promoter construct and by all 5'-deletion constructs except the smallest, Aanat (-217 to +120)luc. Maximal basal and forskolin-stimulated expression levels were generated by the Aanat (-484 to +120)luc construct. This construct lacks a canonical cyclic AMP-response element (CRE), but contains two other potentially important elements in its sequence: an eight times TTATT repeat (TTATT₈) and a CRE-like sequence. Electrophoretic mobility shift assays, luciferase reporter assays, chromatin immunoprecipitation, and siRNA experiments provide evidence that these elements bind c-Fos, JunD, and CREB to enhance basal and forskolin-stimulated Aanat transcription. We propose that the CRE-like sequence and TTATT₈ elements in the 484 bp proximal promoter interact to mediate cAMP-dependent transcriptional regulation of Aanat.

Published
2011

9. Getting rhythm: how do babies do it?

Author

Joseph, Desaline, primary, Chong, Nelson W, additional, Shanks, Morag E, additional, Rosato, Ezio, additional, Taub, Nick A, additional, Petersen, Stewart A, additional, Symonds, Michael E, additional, Whitehouse, William P, additional, and Wailoo, Michael, additional

Published
2014
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12. Characterization of Expressed Sequence Tags From a Gallus gallus Pineal Gland cDNA Library

Author

Hartman, Stefanie, Touchton, Greg, Wynn, Jessica, Geng, Tuoyu, Chong, Nelson W., Smith, Edward J., Hartman, Stefanie, Touchton, Greg, Wynn, Jessica, Geng, Tuoyu, Chong, Nelson W., and Smith, Edward J.

Abstract

The pineal gland is the circadian oscillator in the chicken, regulating diversefunctions ranging from egg laying to feeding. Here, we describe the isolation andcharacterization of expressed sequence tags (ESTs) isolated from a chicken pinealgland cDNA library. A total of 192 unique sequences were analysed and submittedto GenBank; 6% of the ESTs matched neither GenBank cDNA sequences nor thenewly assembled chicken genomic DNA sequence, three ESTs aligned with sequencesdesignated to be on the Z_random, while one matched a W chromosome sequence andcould be useful in cataloguing functionally important genes on this sex chromosome.Additionally, single nucleotide polymorphisms (SNPs) were identified and validatedin 10 ESTs that showed 98% or higher sequence similarity to known chicken genes.Here, we have described resources that may be useful in comparative and functionalgenomic analysis of genes expressed in an important organ, the pineal gland, in amodel and agriculturally important organism.

Published
2005
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13. Characterization of Expressed Sequence Tags From a Gallus gallus Pineal Gland cDNA Library

Author

Animal and Poultry Sciences, Hartman, Stefanie, Touchton, Greg, Wynn, Jessica, Geng, Tuoyu, Chong, Nelson W., Smith, Edward J., Animal and Poultry Sciences, Hartman, Stefanie, Touchton, Greg, Wynn, Jessica, Geng, Tuoyu, Chong, Nelson W., and Smith, Edward J.

Abstract

The pineal gland is the circadian oscillator in the chicken, regulating diversefunctions ranging from egg laying to feeding. Here, we describe the isolation andcharacterization of expressed sequence tags (ESTs) isolated from a chicken pinealgland cDNA library. A total of 192 unique sequences were analysed and submittedto GenBank; 6% of the ESTs matched neither GenBank cDNA sequences nor thenewly assembled chicken genomic DNA sequence, three ESTs aligned with sequencesdesignated to be on the Z_random, while one matched a W chromosome sequence andcould be useful in cataloguing functionally important genes on this sex chromosome.Additionally, single nucleotide polymorphisms (SNPs) were identified and validatedin 10 ESTs that showed 98% or higher sequence similarity to known chicken genes.Here, we have described resources that may be useful in comparative and functionalgenomic analysis of genes expressed in an important organ, the pineal gland, in amodel and agriculturally important organism.

Published
2005

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