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18 results on '"Chong, Jenny Pek Ching"'

1. Prognostic Value of Left Atrial Strain in Aortic Stenosis: A Competing Risk Analysis

Author

Tan, Eugene S.J., Jin, Xuanyi, Oon, Yen Yee, Chan, Siew Pang, Gong, Lingli, Lunaria, Josephine B., Liew, Oi-Wah, Chong, Jenny Pek-Ching, Tay, Edgar L.W., Soo, Wern Miin, Yip, James Wei-Luen, Yong, Quek Wei, Lee, Evelyn Min, Yeo, Daniel Poh-Shuan, Ding, Zee Pin, Tang, Hak Chiaw, Ewe, See Hooi, Chin, Calvin W.L., Chai, Siang Chew, Goh, Ping Ping, Ling, Lee Fong, Ong, Hean Yee, Richards, A. Mark, and Ling, Lieng-Hsi

Published
2023
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5. Large-Scale Whole-Genome Sequencing of Three Diverse Asian Populations in Singapore

Author

Ackers-Johnson, Matthew Andrew, Aliwarga, Edita, Ban, Kenneth Hon Kim, Bertrand, Denis, Chambers, John C., Chan, Dana Leng Hui, Chan, Cheryl Xue Li, Chee, Miao Li, Chee, Miao Ling, Chen, Pauline, Chen, Yunxin, Chew, Elaine Guo Yan, Chew, Wen Jie, Chiam, Lynn Hui Yun, Chong, Jenny Pek Ching, Chua, Ivan, Cook, Stuart A., Dai, Wei, Dorajoo, Rajkumar, Foo, Chuan-Sheng, Goh, Rick Siow Mong, Hillmer, Axel M., Irwan, Ishak D., Jaufeerally, Fazlur, Javed, Asif, Jeyakani, Justin, Koh, John Tat Hung, Koh, Jia Yu, Krishnaswamy, Pavitra, Kuan, Jyn Ling, Kumari, Neelam, Lee, Ai Shan, Lee, Seow Eng, Lee, Sheldon, Lee, Yen Ling, Leong, See Ting, Li, Zheng, Li, Peter Yiqing, Liew, Jun Xian, Liew, Oi Wah, Lim, Su Chi, Lim, Weng Khong, Lim, Chia Wei, Lim, Tingsen Benson, Lim, Choon Kiat, Loh, Seet Yoong, Lok, Au Wing, Chin, Calvin W.L., Majithia, Shivani, Maurer-Stroh, Sebastian, Meah, Wee Yang, Mok, Shi Qi, Nargarajan, Niranjan, Ng, Pauline, Ng, Sarah B., Ng, Zhenyuan, Ng, Jessica Yan Xia, Ng, Ebonne, Ng, Shi Ling, Nusinovici, Simon, Ong, Chin Thing, Pan, Bangfen, Pedergnana, Vincent, Poh, Stanley, Prabhakar, Shyam, Prakash, Kumar M., Quek, Ivy, Sabanayagam, Charumathi, See, Wei Qiang, Sia, Yee Yen, Sim, Xueling, Sim, Wey Cheng, So, Jimmy, Soon, Dinna K.N., Tai, E. Shyong, Tan, Nicholas Y., Tan, Louis C.S., Tan, Hong Chang, Tan, Wilson Lek Wen, Tandiono, Moses, Tay, Amanda, Thakur, Sahil, Tham, Yih Chung, Tiang, Zenia, Toh, Grace Li-Xian, Tsai, Pi Kuang, Veeravalli, Lavanya, Verma, Chandra S., Wang, Ling, Wang, Min Rui, Wong, Wing-Cheong, Xie, Zhicheng, Yeo, Khung Keong, Zhang, Liang, Zhai, Weiwei, Zhao, Yi, Wu, Degang, Dou, Jinzhuang, Chai, Xiaoran, Bellis, Claire, Wilm, Andreas, Shih, Chih Chuan, Soon, Wendy Wei Jia, Bertin, Nicolas, Lin, Clarabelle Bitong, Khor, Chiea Chuen, DeGiorgio, Michael, Cheng, Shanshan, Bao, Li, Karnani, Neerja, Hwang, William Ying Khee, Davila, Sonia, Tan, Patrick, Shabbir, Asim, Moh, Angela, Tan, Eng-King, Foo, Jia Nee, Goh, Liuh Ling, Leong, Khai Pang, Foo, Roger S.Y., Lam, Carolyn Su Ping, Richards, Arthur Mark, Cheng, Ching-Yu, Aung, Tin, Wong, Tien Yin, Ng, Huck Hui, Liu, Jianjun, and Wang, Chaolong

Published
2019
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6. Circulating erythroferrone has diagnostic utility for acute decompensated heart failure in patients presenting with acute or worsening dyspnea

Author

Appleby, Sarah, primary, Frampton, Chris, additional, Holdaway, Mark, additional, Chew-Harris, Janice, additional, Liew, Oi Wah, additional, Chong, Jenny Pek Ching, additional, Lewis, Lynley, additional, Troughton, Richard, additional, Ooi, Shirley Beng Suat, additional, Kuan, Win Sen, additional, Ibrahim, Irwani, additional, Chan, Siew Pang, additional, Richards, A. Mark, additional, and Pemberton, Christopher J., additional

Published
2024
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7. Immunoassay-Compatible Inactivation of SARS-CoV-2 in Plasma Samples for Enhanced Handling Safety

Author

Liew, Oi Wah, primary, Fanusi, Felic, additional, Ng, Jessica Yan Xia, additional, Ahidjo, Bintou Ahmadou, additional, Ling, Samantha Shi Min, additional, Lilyanna, Shera, additional, Chong, Jenny Pek Ching, additional, Lim, Angeline Eng Siew, additional, Lim, Wei Zheng, additional, Ravindran, Sindhu, additional, Chu, Justin Jang Hann, additional, Lim, Shir Lynn, additional, and Richards, Arthur Mark, additional

Published
2022
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10. Mid‐regional pro‐adrenomedullin outperforms N‐terminal pro‐B‐type natriuretic peptide for the diagnosis of acute heart failure in the presence of atrial fibrillation

Author

Kuan, Win Sen, primary, Ibrahim, Irwani, additional, Chan, Siew Pang, additional, Li, Zisheng, additional, Liew, Oi Wah, additional, Frampton, Chris, additional, Troughton, Richard, additional, Pemberton, Chris J., additional, Chong, Jenny Pek Ching, additional, Tan, Li Ling, additional, Lin, Weiqin, additional, Ooi, Shirley Beng Suat, additional, and Richards, A. Mark, additional

Published
2019
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11. Large-Scale Whole-Genome Sequencing of Three Diverse Asian Populations in Singapore

Author

Wu, Degang, primary, Dou, Jinzhuang, additional, Chai, Xiaoran, additional, Bellis, Claire, additional, Wilm, Andreas, additional, Shih, Chih Chuan, additional, Soon, Wendy Wei Jia, additional, Bertin, Nicolas, additional, Lin, Clarabelle Bitong, additional, Khor, Chiea Chuen, additional, DeGiorgio, Michael, additional, Cheng, Shanshan, additional, Bao, Li, additional, Karnani, Neerja, additional, Hwang, William Ying Khee, additional, Davila, Sonia, additional, Tan, Patrick, additional, Shabbir, Asim, additional, Moh, Angela, additional, Tan, Eng-King, additional, Foo, Jia Nee, additional, Goh, Liuh Ling, additional, Leong, Khai Pang, additional, Foo, Roger S.Y., additional, Lam, Carolyn Su Ping, additional, Richards, Arthur Mark, additional, Cheng, Ching-Yu, additional, Aung, Tin, additional, Wong, Tien Yin, additional, Ng, Huck Hui, additional, Liu, Jianjun, additional, Wang, Chaolong, additional, Ackers-Johnson, Matthew Andrew, additional, Aliwarga, Edita, additional, Ban, Kenneth Hon Kim, additional, Bertrand, Denis, additional, Chambers, John C., additional, Chan, Dana Leng Hui, additional, Chan, Cheryl Xue Li, additional, Chee, Miao Li, additional, Chee, Miao Ling, additional, Chen, Pauline, additional, Chen, Yunxin, additional, Chew, Elaine Guo Yan, additional, Chew, Wen Jie, additional, Chiam, Lynn Hui Yun, additional, Chong, Jenny Pek Ching, additional, Chua, Ivan, additional, Cook, Stuart A., additional, Dai, Wei, additional, Dorajoo, Rajkumar, additional, Foo, Chuan-Sheng, additional, Goh, Rick Siow Mong, additional, Hillmer, Axel M., additional, Irwan, Ishak D., additional, Jaufeerally, Fazlur, additional, Javed, Asif, additional, Jeyakani, Justin, additional, Koh, John Tat Hung, additional, Koh, Jia Yu, additional, Krishnaswamy, Pavitra, additional, Kuan, Jyn Ling, additional, Kumari, Neelam, additional, Lee, Ai Shan, additional, Lee, Seow Eng, additional, Lee, Sheldon, additional, Lee, Yen Ling, additional, Leong, See Ting, additional, Li, Zheng, additional, Li, Peter Yiqing, additional, Liew, Jun Xian, additional, Liew, Oi Wah, additional, Lim, Su Chi, additional, Lim, Weng Khong, additional, Lim, Chia Wei, additional, Lim, Tingsen Benson, additional, Lim, Choon Kiat, additional, Loh, Seet Yoong, additional, Lok, Au Wing, additional, Chin, Calvin W.L., additional, Majithia, Shivani, additional, Maurer-Stroh, Sebastian, additional, Meah, Wee Yang, additional, Mok, Shi Qi, additional, Nargarajan, Niranjan, additional, Ng, Pauline, additional, Ng, Sarah B., additional, Ng, Zhenyuan, additional, Ng, Jessica Yan Xia, additional, Ng, Ebonne, additional, Ng, Shi Ling, additional, Nusinovici, Simon, additional, Ong, Chin Thing, additional, Pan, Bangfen, additional, Pedergnana, Vincent, additional, Poh, Stanley, additional, Prabhakar, Shyam, additional, Prakash, Kumar M., additional, Quek, Ivy, additional, Sabanayagam, Charumathi, additional, See, Wei Qiang, additional, Sia, Yee Yen, additional, Sim, Xueling, additional, Sim, Wey Cheng, additional, So, Jimmy, additional, Soon, Dinna K.N., additional, Tai, E. Shyong, additional, Tan, Nicholas Y., additional, Tan, Louis C.S., additional, Tan, Hong Chang, additional, Tan, Wilson Lek Wen, additional, Tandiono, Moses, additional, Tay, Amanda, additional, Thakur, Sahil, additional, Tham, Yih Chung, additional, Tiang, Zenia, additional, Toh, Grace Li-Xian, additional, Tsai, Pi Kuang, additional, Veeravalli, Lavanya, additional, Verma, Chandra S., additional, Wang, Ling, additional, Wang, Min Rui, additional, Wong, Wing-Cheong, additional, Xie, Zhicheng, additional, Yeo, Khung Keong, additional, Zhang, Liang, additional, Zhai, Weiwei, additional, and Zhao, Yi, additional

Published
2019
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12. Sleep study guided multidisciplinary therapy for acute coronary syndrome: a randomized trial

Author

Lee, Chi-Hang Ronald, primary, Koo, Chieh–yang, additional, Kristanto, William, additional, Koh, Esther Hui–ting, additional, Tan, Eugene Siang–joo, additional, Abd Rahman, Shaffinaz, additional, Abd Gani, Munirah Binte, additional, Chong, Jenny Pek–ching, additional, Aung, Aye–thandar, additional, Han, Tun–oo, additional, Chan, Siew–pang, additional, Low, Adrian F., additional, Yeo, Tiong–cheng, additional, Chan, Mark Y., additional, Kojodjojo, Pipin, additional, Richards, Arthur Mark, additional, and Chua, Ai–ping, additional

Published
2019
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13. Large-Scale Whole-Genome Sequencing of Three Diverse Asian Populations in Singapore

Author

Wu, Degang, Dou, Jinzhuang, Chai, Xiaoran, Bellis, Claire, Wilm, Andreas, Shih, Chih Chuan, Soon, Wendy Wei Jia, Bertin, Nicolas, Lin, Clarabelle Bitong, Khor, Chiea Chuen, DeGiorgio, Michael, Cheng, Shanshan, Bao, Li, Karnani, Neerja, Hwang, William Ying Khee, Davila, Sonia, Tan, Patrick, Shabbir, Asim, Moh, Angela, Tan, Eng King, Foo, Jia Nee, Goh, Liuh Ling, Leong, Khai Pang, Foo, Roger S.Y., Lam, Carolyn Su Ping, Richards, Arthur Mark, Cheng, Ching Yu, Aung, Tin, Wong, Tien Yin, Ng, Huck Hui, Ackers-Johnson, Matthew Andrew, Aliwarga, Edita, Ban, Kenneth Hon Kim, Bertrand, Denis, Chambers, John C., Chan, Dana Leng Hui, Chan, Cheryl Xue Li, Chee, Miao Li, Chee, Miao Ling, Chen, Pauline, Chen, Yunxin, Chew, Elaine Guo Yan, Chew, Wen Jie, Chiam, Lynn Hui Yun, Chong, Jenny Pek Ching, Chua, Ivan, Cook, Stuart A., Dai, Wei, Dorajoo, Rajkumar, Foo, Chuan Sheng, Goh, Rick Siow Mong, Hillmer, Axel M., Irwan, Ishak D., Jaufeerally, Fazlur, Javed, Asif, Jeyakani, Justin, Koh, John Tat Hung, Koh, Jia Yu, Krishnaswamy, Pavitra, Kuan, Jyn Ling, Kumari, Neelam, Lee, Ai Shan, Lee, Seow Eng, Lee, Sheldon, Lee, Yen Ling, Leong, See Ting, Li, Zheng, Li, Peter Yiqing, Liew, Jun Xian, Liew, Oi Wah, Lim, Su Chi, Lim, Weng Khong, Lim, Chia Wei, Lim, Tingsen Benson, Lim, Choon Kiat, Loh, Seet Yoong, Lok, Au Wing, Chin, Calvin W.L., Majithia, Shivani, Maurer-Stroh, Sebastian, Meah, Wee Yang, Mok, Shi Qi, Nargarajan, Niranjan, Ng, Pauline, Ng, Sarah B., Ng, Zhenyuan, Ng, Jessica Yan Xia, Ng, Ebonne, Ng, Shi Ling, Nusinovici, Simon, Ong, Chin Thing, Pan, Bangfen, Pedergnana, Vincent, Poh, Stanley, Prabhakar, Shyam, Prakash, Kumar M., Quek, Ivy, Sabanayagam, Charumathi, See, Wei Qiang, Sia, Yee Yen, Sim, Xueling, Sim, Wey Cheng, So, Jimmy, Soon, Dinna K.N., Tai, E. Shyong, Tan, Nicholas Y., Tan, Louis C.S., Tan, Hong Chang, Tan, Wilson Lek Wen, Tandiono, Moses, Tay, Amanda, Thakur, Sahil, Tham, Yih Chung, Tiang, Zenia, Toh, Grace Li Xian, Tsai, Pi Kuang, Veeravalli, Lavanya, Verma, Chandra S., Wang, Ling, Wang, Min Rui, Wong, Wing Cheong, Xie, Zhicheng, Yeo, Khung Keong, Zhang, Liang, Zhai, Weiwei, Zhao, Yi, Liu, Jianjun, Wang, Chaolong, other, and, Wu, Degang, Dou, Jinzhuang, Chai, Xiaoran, Bellis, Claire, Wilm, Andreas, Shih, Chih Chuan, Soon, Wendy Wei Jia, Bertin, Nicolas, Lin, Clarabelle Bitong, Khor, Chiea Chuen, DeGiorgio, Michael, Cheng, Shanshan, Bao, Li, Karnani, Neerja, Hwang, William Ying Khee, Davila, Sonia, Tan, Patrick, Shabbir, Asim, Moh, Angela, Tan, Eng King, Foo, Jia Nee, Goh, Liuh Ling, Leong, Khai Pang, Foo, Roger S.Y., Lam, Carolyn Su Ping, Richards, Arthur Mark, Cheng, Ching Yu, Aung, Tin, Wong, Tien Yin, Ng, Huck Hui, Ackers-Johnson, Matthew Andrew, Aliwarga, Edita, Ban, Kenneth Hon Kim, Bertrand, Denis, Chambers, John C., Chan, Dana Leng Hui, Chan, Cheryl Xue Li, Chee, Miao Li, Chee, Miao Ling, Chen, Pauline, Chen, Yunxin, Chew, Elaine Guo Yan, Chew, Wen Jie, Chiam, Lynn Hui Yun, Chong, Jenny Pek Ching, Chua, Ivan, Cook, Stuart A., Dai, Wei, Dorajoo, Rajkumar, Foo, Chuan Sheng, Goh, Rick Siow Mong, Hillmer, Axel M., Irwan, Ishak D., Jaufeerally, Fazlur, Javed, Asif, Jeyakani, Justin, Koh, John Tat Hung, Koh, Jia Yu, Krishnaswamy, Pavitra, Kuan, Jyn Ling, Kumari, Neelam, Lee, Ai Shan, Lee, Seow Eng, Lee, Sheldon, Lee, Yen Ling, Leong, See Ting, Li, Zheng, Li, Peter Yiqing, Liew, Jun Xian, Liew, Oi Wah, Lim, Su Chi, Lim, Weng Khong, Lim, Chia Wei, Lim, Tingsen Benson, Lim, Choon Kiat, Loh, Seet Yoong, Lok, Au Wing, Chin, Calvin W.L., Majithia, Shivani, Maurer-Stroh, Sebastian, Meah, Wee Yang, Mok, Shi Qi, Nargarajan, Niranjan, Ng, Pauline, Ng, Sarah B., Ng, Zhenyuan, Ng, Jessica Yan Xia, Ng, Ebonne, Ng, Shi Ling, Nusinovici, Simon, Ong, Chin Thing, Pan, Bangfen, Pedergnana, Vincent, Poh, Stanley, Prabhakar, Shyam, Prakash, Kumar M., Quek, Ivy, Sabanayagam, Charumathi, See, Wei Qiang, Sia, Yee Yen, Sim, Xueling, Sim, Wey Cheng, So, Jimmy, Soon, Dinna K.N., Tai, E. Shyong, Tan, Nicholas Y., Tan, Louis C.S., Tan, Hong Chang, Tan, Wilson Lek Wen, Tandiono, Moses, Tay, Amanda, Thakur, Sahil, Tham, Yih Chung, Tiang, Zenia, Toh, Grace Li Xian, Tsai, Pi Kuang, Veeravalli, Lavanya, Verma, Chandra S., Wang, Ling, Wang, Min Rui, Wong, Wing Cheong, Xie, Zhicheng, Yeo, Khung Keong, Zhang, Liang, Zhai, Weiwei, Zhao, Yi, Liu, Jianjun, Wang, Chaolong, and other, and

Abstract

Because of Singapore's unique history of immigration, whole-genome sequence analysis of 4,810 Singaporeans provides a snapshot of the genetic diversity across East, Southeast, and South Asia.

Published
2019

14. Mid-regional pro-adrenomedullin outperforms N-terminal pro-B-type natriuretic peptide for the diagnosis of acute heart failure in the presence of atrial fibrillation.

Author

Kuan, Win Sen, Ibrahim, Irwani, Chan, Siew Pang, Li, Zisheng, Liew, Oi Wah, Frampton, Chris, Troughton, Richard, Pemberton, Chris J., Chong, Jenny Pek Ching, Tan, Li Ling, Lin, Weiqin, Ooi, Shirley Beng Suat, and Richards, A. Mark

Subjects
ATRIAL fibrillation, BRAIN natriuretic factor, DIAGNOSIS, HOSPITAL emergency services, ATRIAL fibrillation diagnosis, RESEARCH, RESEARCH methodology, PROTEIN precursors, MEDICAL cooperation, EVALUATION research, COMPARATIVE studies, RESEARCH funding, PEPTIDE hormones, HEART failure, PEPTIDES, DISEASE complications
Abstract

Aims: The performance of N-terminal pro-B-type natriuretic peptide (NT-proBNP) in diagnosing acute decompensated heart failure (ADHF) among patients presenting with breathlessness is markedly impaired in the presence of atrial fibrillation (AF). We evaluated the diagnostic performance of mid-regional pro-adrenomedullin (MR-proADM) and cardiac troponin T as possible alternative markers for discrimination of ADHF in this setting.Methods and Results: Breathless patients (n = 1107) were prospectively and contemporaneously recruited in emergency departments in Singapore and New Zealand. The diagnoses of ADHF and presence of AF were adjudicated by two clinician specialists, blinded to MR-proADM, NT-proBNP and high-sensitivity cardiac troponin T (hs-cTnT) results. MR-proADM exhibited strong discrimination of ADHF with little change in performance irrespective of the presence of AF (area under the curve 0.83 in non-AF vs. 0.76 in AF) compared to NT-proBNP (0.91 vs. 0.71) and hs-cTnT (0.83 vs. 0.62), respectively. The accuracy of MR-proADM (73.3%) for diagnosing ADHF among patients with AF was superior to both NT-proBNP (61.6%) and hs-cTnT (64.6%). The superior performance of MR-proADM remained apparent when data from Singapore and New Zealand were analysed separately.Conclusion: In the presence of AF, MR-proADM showed greater discrimination and accuracy, and less impairment in performance compared to that in non-AF cases, for the diagnosis of ADHF, compared to the guideline-endorsed NT-proBNP. [ABSTRACT FROM AUTHOR]

Published
2020
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15. Sleep study-guided multidisciplinary therapy (SGMT) for patients with acute coronary syndrome: Trial rationale and design

Author

Chua, Ai-Ping, primary, Koo, Chieh-Yang, additional, Kristanto, William, additional, Parot, Maria Victoria Jane Macalalag, additional, Tan, Eugene Siang-Joo, additional, Koh, Esther Hui-Ting, additional, Abd Gani, Munirah Binte, additional, Kojodjojo, Pipin, additional, Han, Tun-Oo, additional, Chan, Siew-Pang, additional, Chong, Jenny Pek-Ching, additional, Frampton, Christopher, additional, Richards, Arthur Mark, additional, and Lee, Chi-Hang, additional

Published
2018
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16. Superior performance of N‐terminal pro brain natriuretic peptide for diagnosis of acute decompensated heart failure in an Asian compared with a Western setting

Author

Ibrahim, Irwani, primary, Kuan, Win Sen, additional, Frampton, Chris, additional, Troughton, Richard, additional, Liew, Oi Wah, additional, Chong, Jenny Pek Ching, additional, Chan, Siew Pang, additional, Tan, Li Ling, additional, Lin, Wei Qin, additional, Pemberton, Chris J., additional, Ooi, Shirley Beng Suat, additional, and Richards, A. Mark, additional

Published
2016
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17. Circulating Plasma Proteins in Aortic Stenosis: Associations With Severity, Myocardial Response, and Clinical Outcomes.

Author

Tan ESJ, Choi H, DeFilippi CR, Oon YY, Chan SP, Gong L, Lunaria JB, Liew OW, Chong JP, Tay EL, Soo WM, Yip JW, Yong QW, Lee EM, Daniel Yeo PS, Ding ZP, Tang HC, Ewe SH, Chin CWL, Chai SC, Goh PP, Ling LF, Ong HY, Richards AM, and Ling LH

Subjects
Humans, Male, Female, Aged, Prospective Studies, Middle Aged, Proteomics methods, Ventricular Function, Left physiology, Prognosis, Stroke Volume physiology, Echocardiography, Disease Progression, Heart Failure blood, Heart Failure physiopathology, Risk Factors, Follow-Up Studies, Aortic Valve Stenosis blood, Aortic Valve Stenosis physiopathology, Aortic Valve Stenosis diagnostic imaging, Severity of Illness Index, Biomarkers blood, Blood Proteins analysis
Abstract

Background: Echocardiographic indexes of aortic stenosis may not comprehensively reflect disease morbidity. Plasma proteomic profiling may add prognostic value in these patients., Methods and Results: Proximity extension assays (Olink) of 183 circulating cardiovascular and inflammatory proteins were performed in a prospective follow-up study of 122 asymptomatic/minimally symptomatic patients (mean±SD age, 69.1±10.9 years; 61% men) with moderate to severe aortic stenosis and preserved left ventricular ejection fraction. Protein signatures of higher-risk echocardiographic subgroups were determined. Associations of proteins with the primary composite outcome (heart failure hospitalization, progression to New York Heart Association class III-IV, or all-cause mortality) were evaluated using competing risk analyses, with aortic valve replacement being the competing risk. Network analysis unveiled mutually exclusive communities of proteins and echocardiographic parameters, connected only through NT-proBNP (N-terminal pro-B-type natriuretic peptide). Members of the tumor necrosis factor receptor superfamily (TNFRSF1A, TNFRSF1B, and TNFRSF14), and trefoil factor-3 were major hub proteins among the circulating biomarkers. Left ventricular global longitudinal strain >-15% was associated with higher levels of proteins, primarily of inflammation and immune regulation, whereas aortic valve area <1 cm 2 , E/e' >15, and left atrial reservoir strain <20% were associated with higher levels of NT-proBNP. Of 14 proteins associated with the primary end point, phospholipase-C, C-X-C motif chemokine-9, and interleukin-10 receptor subunit β demonstrated the highest hazard ratios after adjusting for clinical factors ( q <0.05)., Conclusions: Plasma proteins involved in inflammation and immune regulation were differentially expressed in patients with aortic stenosis with reduced left ventricular global longitudinal strain, and associated with adverse clinical outcomes. Their incorporation into aortic stenosis risk stratification warrants further assessment.

Published
2024
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18. One-Shot Generation of Epitope-Directed Monoclonal Antibodies to Multiple Nonoverlapping Targets: Peptide Selection, Antigen Preparation, and Epitope Mapping.

Author

Liew OW, Ling SSM, Lilyanna S, Chong JPC, Ng JYX, and Richards AM

Subjects
Amino Acid Sequence, Animals, Antigens, Epitope Mapping methods, Epitopes, Peptides genetics, Thioredoxins genetics, Antibodies, Monoclonal genetics, Peptide Library
Abstract

This chapter describes an epitope-directed approach to generate antipeptide monoclonal antibodies to multiple nonoverlapping protein sites using a cocktail of fusion peptides as immunogen. It provides a step-by-step protocol on how antigenic peptides on a target protein can be identified by in silico prediction and discusses considerations for final peptide selection. Each antigenic peptide (10-20 amino acids long) is displayed as three-copy inserts on the surface exposed loop of a thioredoxin scaffold protein. The corresponding DNA coding sequence specifying the tripeptide insert flanked by Gly-Ser-Gly-Ser-Gly linkers is cloned in-frame into the Rsr II site of the thioredoxin gene in the pET-32a vector. The presence of a C-terminal polyhistidine tag (His 6 -tag) allows the soluble fusion proteins to be purified by one-step native immobilized metal affinity chromatography (IMAC) to greater than 95% purity. Multiple thioredoxin fusion proteins are mixed in equimolar concentrations and used as an immunogen cocktail for animal immunization. The use of short antigenic peptides of known sequence facilitates direct epitope mapping requiring only small mutagenesis scan peptide libraries in the multipin peptide format., (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2023
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