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15 results on '"Chondrokouki, Eleni"'

2. Complete genome reconstruction of the global and European regional dispersal history of the lumpy skin disease virus

Author

Van Borm, Steven, primary, Dellicour, Simon, additional, Martin, Darren P., additional, Lemey, Philippe, additional, Agianniotaki, Eirini I., additional, Chondrokouki, Eleni D., additional, Vidanovic, Dejan, additional, Vaskovic, Nikola, additional, Petroviċ, Tamaš, additional, Laziċ, Sava, additional, Koleci, Xhelil, additional, Vodica, Ani, additional, Djadjovski, Igor, additional, Krstevski, Kiril, additional, Vandenbussche, Frank, additional, Haegeman, Andy, additional, De Clercq, Kris, additional, and Mathijs, Elisabeth, additional

Published
2023
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3. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

European Commission, Gallardo, Carmina [0000-0003-3293-306X], Djadjovski, Igor [0000-0002-4820-3590], Krivko, Laura [0009-0000-3047-4061], Chondrokouki, Eleni [0000-0002-8476-1821], Frant, Maciej [0000-0002-7169-3735], Pileviciene, Simona [0000-0002-0262-3899], Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueño, Encarnación, Nieto, Raquel, Pérez, Covadonga, Simón, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milicevik, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Václavek, Petr, Pileviciene, Simona, Marisa, Arias, European Commission, Gallardo, Carmina [0000-0003-3293-306X], Djadjovski, Igor [0000-0002-4820-3590], Krivko, Laura [0009-0000-3047-4061], Chondrokouki, Eleni [0000-0002-8476-1821], Frant, Maciej [0000-0002-7169-3735], Pileviciene, Simona [0000-0002-0262-3899], Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueño, Encarnación, Nieto, Raquel, Pérez, Covadonga, Simón, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milicevik, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Václavek, Petr, Pileviciene, Simona, and Marisa, Arias

Abstract

African swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas.

Published
2023

6. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueno, Encarnacion, Nieto, Raquel, Perez, Covadonga, Simon, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milićević, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Vaclavek, Petr, Pileviciene, Simona, Marisa, Arias, Gallardo, Carmina, Casado, Nadia, Soler, Alejandro, Djadjovski, Igor, Krivko, Laura, Madueno, Encarnacion, Nieto, Raquel, Perez, Covadonga, Simon, Alicia, Ivanova, Emiliya, Donescu, Daniel, Milićević, Vesna, Chondrokouki, Eleni, Nurmoja, Imbi, Frant, Maciej, Feliziani, Francesco, Vaclavek, Petr, Pileviciene, Simona, and Marisa, Arias

Abstract

IntroductionAfrican swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas. Materials and methodsTo further our understanding of the epidemiology and spread of ASFV in Europe, 382 isolates collected during 2007 to 2022 were sequenced. The study was initially performed by sequencing the central variable region (CVR), the intergenic region (IGR) between the I73R and I329L genes and the O174L and K145R genes. For further discrimination, two new PCRs were designed to amplify the IGR between the 9R and 10R genes of the multigene family 505 (MGF505) and the IGR between the I329L and I215L genes. The sequences obtained were compared with genotype II isolates from Europe and Asia. ResultsThe combination of the results obtained by sequencing these variable regions allowed to differentiate the European II-ASFV genotypes into 24 different groups. In addition, the SNP identified in the IGR I329L-I215L region, not previously described, grouped the viruses from North Macedonia that caused the 2022 outbreaks with viruses from Romania, Bulgaria, Serbia and Greece, differentiating from other genotype II isolates present in Europe and Asia. Furthermore, tandem repeat sequence (TRS) within the 9R-10R genes of the multigene family 505 (MGF505) revealed eight different variants circulating. DiscussionThese findings describe a new multi-gene approach sequencing method that can be used in routine genotyping to determine the origin of new introductions in A

Published
2023

7. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Gallardo, Carmina, primary, Casado, Nadia, additional, Soler, Alejandro, additional, Djadjovski, Igor, additional, Krivko, Laura, additional, Madueño, Encarnación, additional, Nieto, Raquel, additional, Perez, Covadonga, additional, Simon, Alicia, additional, Ivanova, Emiliya, additional, Donescu, Daniel, additional, Milicevik, Vesna, additional, Chondrokouki, Eleni, additional, Nurmoja, Imbi, additional, Frant, Maciej, additional, Feliziani, Francesco, additional, Václavek, Petr, additional, Pileviciene, Simona, additional, and Marisa, Arias, additional

Published
2023
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9. Efficacy of oral rabies vaccination in individual age groups of juvenile red foxes

Author

Papatheodorou, Dimos P., primary, Tasioudi, Konstantia E., additional, Korou, Laskarina-Maria, additional, Georgiou, Vasileios L., additional, Markantonatos, Gerasimos, additional, Iliadou, Peristera, additional, Kirtzalidou, Aikaterini, additional, Katsifa, Aggeliki, additional, Ztrivas, Spyridon, additional, Bechtsi, Georgia, additional, Tzani, Myrsini, additional, Chondrokouki, Eleni, additional, and Mangana-Vougiouka, Olga, additional

Published
2018
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10. Colostrum transfer of neutralizing antibodies against lumpy skin disease virus from vaccinated cows to their calves

Author

Agianniotaki, Eirini I., primary, Babiuk, Shawn, additional, Katsoulos, Panagiotis-Dimitrios, additional, Chaintoutis, Serafeim C., additional, Praxitelous, Anastasia, additional, Quizon, Kaye, additional, Boscos, Constantinos, additional, Polizopoulou, Zoe S., additional, Chondrokouki, Eleni D., additional, and Dovas, Chrysostomos I., additional

Published
2018
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11. First four Oral Rabies Vaccination campaigns of the red foxes in Greece: Evaluating factors and assessment

Author

Papatheodorou, Dimos P., primary, Tasioudi, Konstantia E., additional, Korou, Laskarina-Maria, additional, Georgiou, Vasileios, additional, Iliadou, Peristera, additional, Markantonatos, Gerasimos, additional, Kirtzalidou, Aikaterini, additional, Tzani, Myrsini, additional, Chondrokouki, Eleni, additional, and Mangana-Vougiouka, Olga, additional

Published
2018
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13. Complete Genome Sequence of the Lumpy Skin Disease Virus Isolated from the First Reported Case in Greece in 2015

Author

Agianniotaki, Eirini I., primary, Mathijs, Elisabeth, additional, Vandenbussche, Frank, additional, Tasioudi, Konstantia E., additional, Haegeman, Andy, additional, Iliadou, Peristera, additional, Chaintoutis, Serafeim C., additional, Dovas, Chrysostomos I., additional, Van Borm, Steven, additional, Chondrokouki, Eleni D., additional, and De Clercq, Kris, additional

Published
2017
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14. Complete genome reconstruction of the global and European regional dispersal history of the lumpy skin disease virus.

Author

Borm, Steven Van, Dellicour, Simon, Martin, Darren P., Lemey, Philippe, Agianniotaki, Eirini I., Chondrokouki, Eleni D., Vidanovic, Dejan, Vaskovic, Nikola, Petroviċ, Tamaš, Laziċ, Sava, Koleci, Xhelil, Vodica, Ani, Djadjovski, Igor, Krstevski, Kiril, Vandenbussche, Frank, Haegeman, Andy, De Clercq, Kris, and Mathijs, Elisabeth

Subjects
*LUMPY skin disease, *VIRUS diseases, *GENETIC variation, *GENOMES, *WHOLE genome sequencing, *NUCLEOTIDE sequencing
Abstract

Lumpy skin disease virus (LSDV) causes a disease of economic importance affecting cattle. Its global epidemiology is complex due to the combination of vectorborne and anthropogenic spread, the circulation of vaccine-like recombinants, and the use of vaccines. The slow molecular evolution of its DNA genome limits the utility of genetic variation for accurate tracing based on evolutionary analyses, but this limitation has not yet been formally assessed. Furthermore, until present, whole genome sequencing in affected areas has remained patchy. This study combines the first fine-grained sampling of LSDV whole genomes from a time-constrained (2015-2017) southeastern European (SEE) LSDV outbreak, which we analyze along with curated public genomes to investigate the global and regional viral dispersal dynamics. First, haplotype networks visualizing the limited genetic variability associated with the SEE LSDV outbreak show intense intermixing between countries. We also assess at which spatial scale a correlation between genetic and geographic distances can be detected for LSDV. On a global scale, we show the importance of accounting for recombination events that can impact phylogenetic and phylogeographic reconstructions. Following the assessment of the temporal signal in the recombination-free alignment, our time-scaled continuous phylogeographic analysis of Kenya-like and recent wild-type viruses confirms the origin and global dissemination history of LSDV. Our analyses highlight the importance of careful selection and application of phylodynamic approaches to DNA viruses, as well as the importance of whole genome sampling in endemic and outbreak areas to improve our understanding of the evolution, epidemiology, and transmission dynamics of DNA viruses. [ABSTRACT FROM AUTHOR]

Published
2023
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15. A multi gene-approach genotyping method identifies 24 genetic clusters within the genotype II-European African swine fever viruses circulating from 2007 to 2022

Author

Carmina Gallardo, Nadia Casado, Alejandro Soler, Igor Djadjovski, Laura Krivko, Encarnación Madueño, Raquel Nieto, Covadonga Perez, Alicia Simon, Emiliya Ivanova, Daniel Donescu, Vesna Milicevik, Eleni Chondrokouki, Imbi Nurmoja, Maciej Frant, Francesco Feliziani, Petr Václavek, Simona Pileviciene, Arias Marisa, European Commission, Gallardo, Carmina, Djadjovski, Igor, Krivko, Laura, Chondrokouki, Eleni, Frant, Maciej, and Pileviciene, Simona

Subjects
Genotyping, General Veterinary, SNP, TRS, ASFV, Genetic groups
Abstract

14 Pág., African swine fever (ASF) is a contagious viral disease of pigs and wild boar that poses a major threat to the global swine industry. The genotype II African swine fever virus (ASFV) entered the European Union (EU) in 2014 and since then fourteen countries have been affected, Italy and North Macedonia being the last in 2022. While whole genome sequencing remains the gold standard for the identification of new genetic markers, sequencing of multiple loci with significant variations could be used as a rapid and cost-effective alternative to track outbreaks and study disease evolution in endemic areas., This study was supported by the European Union Reference laboratory for ASF (grant no. UE- LR PPA/03).

Published
2023

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