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Background and aims Greater egg consumption is associated with poor glycemic control. However, greater egg intake could simply be a marker for other dietary indiscretions such as greater intake of energy or saturated fat, which may influence glycemic control. We examined the association between egg consumption and total energy intake and saturated fat intake, measured using the remote food photography method. Methods An observational study was conducted with 48 healthy adults consuming ≥1 eggs/week. Photographs of all meals consumed for seven days were captured via a smartphone application called SmartIntake® and analyzed for nutrient intake. Fasting blood samples were collected. Linear correlations between egg consumption and nutrient intake or measures of glycemic control were determined. Results Daily egg intake was significantly associated with cholesterol intake, but not with the daily energy and saturated fat intake or measures of glycemic control. Conclusions Higher consumption of eggs in free-living conditions was not associated with undesirable profiles of macronutrient intake, total EI, or an impaired glycemic profile in this study population. Thus, the assertion that eggs are linked with impaired glycemic control because of the accompanying intake of greater energy and saturated fat, does not appear valid. Trial registration NCT 03404700.

Background Smith-Lemli-Opitz syndrome (SLOS) is a rare genetic neurodevelopmental disorder caused by the defect in the 7-dehydrocholesterol reductase. This defect leads to the deficiency of cholesterol biosynthesis with accumulation of 7-dehydrocholesterol. Inhibitory factor 1 (IF1) is a well-known mitochondrial protein. Recently, it has been discovered in the human serum where it is reported to be involved in the HDL-cholesterol intake. Here we report the IF1 presence in the serum of two paediatric SLOS dizygotic twins treated with dietary cholesterol supplementation. Case presentation The patients showed a typical phenotype. They started dietary supplementation with cholesterol when 2 months old. The cholesterol intake was periodically titrated on the basis of weight increase and the twin 1 required a larger supplementation than the twin 2 during the follow-up. When 6.4-year-old, they underwent IF1 assay that was 7-fold increased in twin 2 compared to twin 1 (93.0 pg/ml vs 13.0 pg/ml, respectively). Conclusions We report, for the first time, the presence of circulating IF1 in the serum of SLOS patients, showing different levels among them. Our findings confirm that IF1 could be a novel research target in cholesterol-related disorders and also in SLOS, and could contribute to the general debate on IF1 as a new modulator of cholesterol levels.

Purpose of review We have focused on recent research relevant to effects of dietary patterns and major food groups on cardiovascular outcomes, taking into account guidelines and position statements from expert authorities, with an emphasis on important changes in recommendations, some of which remain controversial. Recent findings Major findings include: refocusing on qualitative patterns of food consumption replacing quantitative prescriptive advice on nutrients; increasing intake of plant foods; substituting saturated fats with polyunsaturated and monounsaturated oils; reducing salt intake; regular consumption of fish with a focus on omega-3 enrichment; not restricting dairy foods, other than butter and cream, with encouragement of some fermented products; reducing cholesterol intake for those at increased cardiovascular risk and diabetes, allowing 7-eggs weekly; restricting processed meats and allowing moderate lean meat consumption; preference for fiber-rich complex carbohydrates and reduced sugar intake; maintaining healthy bodyweight; and although water is the preferred beverage, allowing moderate alcohol consumption to national guidelines and avoiding alcohol in specific cardiovascular disorders. Summary The new approach that focuses on healthier patterns of food intake is more readily understood by health practitioners and translatable to consumers and patients.

Objective: This cross-sectional study aimed to evaluate the association of dietary fatty acids, cholesterol and omega-6/omega-3 ratio to depressive symptoms in a sample of low-risk pregnant women. ...

Fat and cholesterol are an important group of nutrients that should be limited in the diet to reduce the risk of chronic diseases. Cardiovascular and carcinogenic diseases are associated with fat-rich diets, but also other chronic health problems can be exacerbated by high-fat diet. Recommendations promoted by the World Health Organization worldwide are reducing fat intake, primarily saturated fatty acids, trans-fatty acids and cholesterol as a way to minimize health risks. All of these recommendations, daily presented to the public, give consumers awareness to take care of their diet.

Objectives: There has been insufficient evidence for a quantitative recommendation for dietary cholesterol, therefore, we aim to investigate the optimal cholesterol intake related to a lower all-cause mortality risk. Methods: The National Health and Nutrition Examination Survey (NHANES) is a large population survey to investigate public health in the United States. We analysed data from 1999-2002 linked with mortality data obtained through 2006. Cox proportional hazard ratios (HRs) with 95% confidence intervals (CIs) were estimated to assess risks for all-cause mortality associated with cholesterol intake. Results: A total of 7,728 participants were analysed in the present study, and 519 assumed death events. Compared with the third quartile (216-373 mg/day) of cholesterol intake, the risks of mortality increased in both the first two and the last quartiles (quartile 1: HR 1.53, 95% CI 1.16-2.00; quartile 2: HR 1.22, 95% CI 0.94-1.60; quartile 4: HR 1.39, 95% CI 1.05-1.83). The association between cholesterol intake and the risk of all-cause mortality followed a U-shaped curve, with the cholesterol intake associated with the lowest mortality being 328 mg/day. Conclusions: The present study suggests an optimal cholesterol intake for lowering the all-cause mortality risk.

BACKGROUND: Little is known about associations between dietary inflammation, age and anthropometric measurements. OBJECTIVE: In this regard, we examine how DII is related to age, anthropometrics [weight, Body mass index (BMI), waist to hip ratio (WHR)] and other parameters of nutrition (energy, protein, fats and cholesterol intake, Net Endogenous Acid Production (NEAP) and Phytochemical Index (PI)) in a cross-sectional study in Pakistan. DESIGN: Only men (n = 651, age 54–95 years) participated in the study. Anthropometric data were collected using standard methods. DII was calculated from nutrients derived from 24-hr Dietary Recall questionnaires. NEAP and PI were calculated by established algorithms using information on nutrient intake. RESULTS: The results show that with increasing age, there was a significant increase in the DII score (p 0.05). Similarly, a significant positive correlation with NEAP (p

A model was developed that can be used to study the effect of gradual cholesterol intake by food on the HPA axis dynamics. Namely, well defined oscillatory dynamics of vital neuroendocrine hypothalamic-pituitary-adrenal (HPA) axis has proven to be necessary for maintaining regular basal physiology and formulating appropriate stress response to various types of perturbations. Cholesterol, as a precursor of all steroid HPA axis hormones, can alter the dynamics of HPA axis. To analyse its particular influence on the HPA axis dynamics we used stoichiometric model of HPA axis activity, and simulate cholesterol perturbations in the form of finite duration pulses, with asymmetrically distributed concentration profile. Our numerical simulations showed that there is a complex, nonlinear dependence between the HPA axis responsiveness and different forms of applied cholesterol concentration pulses, indicating the significance of kinetic modelling, and dynamical systems theory for the understanding of large-scale self-regulatory, and homeostatic processes within this neuroendocrine system.

Cigarette smoke contains nicotine which disturbs health. Nicotine is capable of reducing the taste of food, so there is appetite suppressant. The ability of nicotine in increasing blood liposuction process can affect the increase in blood cholesterol level. The research aims to study the difference between food intake and blood cholesterol level between smokers and non-smokers. This study was a comparative observational analytic study using cross-sectional design. The data of particular subjects and smoking habit were obtained from the questionnaire. The food intake was obtained by interview using 2x24 hours food recall form while the blood cholesterol level was obtained by blood sampling which was then analyzed using laboratory test. The subjects were 13 smokers and 13 non-smokers, taken by simple random sampling. The data were analyzed by Independent t-Test. The results have suggested that the difference in the average food intake and blood cholesterol level between smokers and non-smokers were not significant. The substantial average difference was found in the level of cholesterol intake, so the statistical test has showed a significant difference , p-value = 0.005 < α (0.05). Smoking has a harmful effect for health so people must decrease its consumption or stay abstinent from it. The longer human get exposed to cigarette, the more susceptible they get the illness from it.

Childhood obesity is an undeniable reality and has shown a rapid growth in many countries. Obesity at an early age not only increases the risks of chronic diseases but also produces a problem for the whole healthcare system. One way to alleviate this problem is to provide each patient with an appropriate menu that can be defined with a mathematical model. Existing mathematical models only partially address the objective and constraints of childhood obesity; therefore, the solutions provided are insufficient for health specialists to prepare nutritional menus for individual patients. This manuscript proposes a multiobjective mathematical programming model to aid healthy nutritional menu planning to prevent childhood obesity. This model enables a plan for combinations and amounts of food across different schedules and daily meals. This approach minimizes the major risk factors of childhood obesity (i.e., glycemic load and cholesterol intake). In addition, it considers the minimization of nutritional mismatch and total cost. The model is solved using a deterministic method and two metaheuristic methods. To complete this numerical study, test instances associated with children aged 4-18 years old were created. The quality of the solutions generated using the three methods was similar, but the metaheuristic methods provided solutions in less computational time. The numerical results indicate proper guidelines for personalized plans for individual children.

Childhood obesity is an undeniable reality that has rapidly increased in many countries. Obesity at an early age not only increases the risks of chronic diseases but also produces a problem for the whole healthcare system. One way to alleviate this problem is to provide each patient with an appropriate menu that is defined by a mathematical model. Existing mathematical models only partially address the objective and constraints of childhood obesity; therefore, the solutions provided are insufficient for health specialists to prepare nutritional menus for individual patients. This manuscript proposes a multiobjective mathematical programming model to aid in healthy nutritional menu planning that may prevent childhood obesity. This model provides a plan for combinations and amounts of food across different schedules and daily meals. This approach minimizes the major risk factors of childhood obesity (i.e., glycemic load and cholesterol intake). In addition, this approach considers the minimization of nutritional mismatch and total cost. The model is solved using a deterministic method and two metaheuristic methods. Test instances associated with children aged 4-18 years were created with the support of health professionals to complete this numerical study. The quality of the solutions generated using the three methods was similar, but the metaheuristic methods provided solutions in a shorter computational time. These results are submitted to statistical hypothesis tests to be validated. The numerical results indicate proper guidelines for personalized plans for individual children.

It remains unclear whether cholesterol intake can increase serum cholesterol. Therefore, the present study aimed to investigate the hypothesis that the risk for hypercholesterolemia was not associated with intake of dietary cholesterol after adjusting for saturated fatty acid (SFA). Based on the data from the 2012&ndash, 2016 KNHANES, dietary cholesterol was positively associated with the risk for abnormalities in total cholesterol (TC) (odds ratio (OR): 1.153, 95% confidence interval (CI): 0.995&ndash, 1.337, p = 0.028) and low-density lipoprotein cholesterol (LDL-C) (OR: 1.186, 95% CI: 1.019&ndash, 1.382, p = 0.018) levels before adjusting for SFA, after adjusting for SFA, no significant associations were found between these variables. The mediation analysis showed that dietary cholesterol had no direct effects on the serum levels of TC and LDL-C, in contrast, SFA had significant indirect effects on the association between dietary cholesterol and serum levels of TC and LDL-C. Furthermore, processed meats, but not eggs and other meats, were positively associated with the risk for abnormalities in both TC (OR: 1.220, 95% CI: 1.083&ndash, 1.374, p = 0.001) and LDL-C (OR: 1.193, 95% CI: 1.052&ndash, 1.354, p = 0.004) levels. The present study suggested that higher intake of processed meats with high SFA, but not dietary cholesterol was associated with higher risk for abnormalities in TC and LDL-C levels.

The 2015 Dietary Guidelines for Americans recommends that individuals should eat as little dietary cholesterol as possible. However, current dietary cholesterol intake and its food sources have not been well-characterized. We examined dietary cholesterol intake by age, sex, race, and food sources using 24-hour dietary recall data from a nationally representative sample of 5047 adults aged 20 years or older who participated in NHANES (2013–2014 survey cycle). We also reported trends in cholesterol intake across the past 7 NHANES surveys. Mean dietary cholesterol intake was 293 mg/day (348 mg/day for males and 242 mg/day for females) in the 2013–2014 survey cycle; 39% of adults had dietary cholesterol intake above 300 mg/day (46% for males and 28% for females). Meat, eggs, grain products, and milk were the highest four food sources of cholesterol, contributing to 96% of the total consumption. Both average cholesterol intake and food source varied by age, sex, and race (each p < 0.05). Mean cholesterol intake of the overall population had been relatively constant at ~290 mg/day from 2001–2002 to 2013–2014 (p-trend = 0.98). These results should inform public health efforts in implementing dietary guidelines and tailoring dietary recommendations.

The goal of the present study was to examine dietary adherence and acceptability among participants from the New DIETs study who were randomized to one of four plant-based diets (vegan, vegetarian, pesco-vegetarian, semi-vegetarian) or an omnivore diet. Primary outcomes at two- and six months included dietary adherence (24-hour dietary recalls), weight loss and changes in animal product intake (mg cholesterol) by adherence status, Three-Factor Eating Questionnaire (TFEQ), Power of Food Scale (PFS), dietary acceptability (Food Acceptability Questionnaire), and impact of diet preference on adherence. No differences were found in dietary adherence or changes in FAQ, TFEQ, or PFS among the groups. At six months, non-adherent vegan and vegetarian participants (n=16) had a significantly greater decrease in cholesterol intake (-190.2 ± 199.2 mg) than non-adherent pesco-vegetarian/semi-vegetarian (n=15, -2.3 ± 200.3 mg, P=0.02) or omnivore participants (n=7, 17.0 ± 36.0, P=0.04). Non-adherent vegan/vegetarian participants lost significantly more weight at six months (-6.0 ± 6.7%) than non-adherent omnivore participants (-0.4 ± 0.6%, P=0.04). Dietary preference had no impact on adherence at six months. Due to equal rates of adherence and acceptability among the diet groups, instructing participants to follow vegan or vegetarian diets may have a greater impact on weight loss and animal product intake than providing instruction in more moderate approaches even among non-adherent participants.

Introduction The older literature (mid 1980-90) shows that increased dietary cholesterol intake can raise total serum cholesterol and LDL levels., Some more current positions question the recommendation of daily cholesterol intake, as well as the impact of the whole chicken egg, inquiring whether it is a harmful or beneficial classification food in this context., In the midst of controversial issues, it is essential to analyze variables such as food consumption in general and how much dietary cholesterol intake is impacting [...]

Consumers make dietary choices based on information they have. This study examines consumer choice of food products considering the healthfulness of multiple nutrients. We focus on high levels of sugars because higher levels of intake are associated with adverse health and nutritional outcomes. We find an important association of sugar consumption with the healthfulness of a diet based on saturated fat intake and cholesterol intake. Consumers making healthier choices in one nutrient are not making healthier choices on other nutrients. Our results suggest that individuals who were making better dietary choices based on saturated fats were consuming more sugars. This could indicate challenges with the existing standard nutrition label as an information tool towards making healthful choices on multiple nutrients.

Department of Animal Products and Food Science, Kangwon National University, Chuncheon 200-701, KoreaABSTRACT The objective of this literature review is to prove that egg cholesterol is not related with heart disease based upon research results of the past 50 years. As a result, it was reported that the dietary intake cholesterol did not affected cholesterol levels in blood. Moreover, it was reported that adding two eggs a day to a healthful diet did not significantly increase blood cholesterol levels in young or middle-aged men and women with normal or even moderately elevated blood cholesterol levels. Cholesterol feeding studies demonstrate that dietary cholesterol increases both LDL and HDL cholesterol with little change in the LDL:HDL ratio as an important risk indicator of heart disease. Especially a good indicator in the elderly from increased HDL-cholesterol, so cholesterol intake appeared to be good for the prevention of heart disease. Thus the consumption of eggs is bad for your health is not known as a fact, but rather was found to be effective in preventing heart disease. Also, all consumers may consume without worrying because there are no official standards regarding egg intake. Therefore, these evidences would clear up some misunderstanding and mistrust on egg cholesterol regarding the consumption of egg as well as give some desirable advice on how to maintain healthy life.(Key words : egg, heart disease, HDL-cholesterol, LDL-cholesterol)

Summary Objective The objective of this study was to estimate the total fat, saturated, monounsaturated, polyunsaturated fatty acids and cholesterol intake by sportsmen practicing slalom canoe racing and sport-shooting. Material and methods The studies were performed at 29 slalom canoe's racing and at 23 sport's shooting sportsmen. Twenty-four-hour dietary recalls were collected in training and start period of 2009, for three selected days per week (including Friday and Sunday, due to the different, eating habits in Poland). Results The obtained amount of total fats intake in average daily diet of canoeist and shooters were 29% and 34% of total energy, respectively. The saturated fatty acids delivered –16% and 17% of total energy, respectively. The percentage of monounsaturated fatty acids was in accordance with recommendations (above 10% of total energy of daily diets), whereas for n-3 family polyunsaturated fatty acids, especially the docosahexaenoic acid and eicosapentaenoic acid, were too low (below 1% of energy of daily diets). However the proper ratio of n-6 to n-3 (5–6:1) were found. Conclusions It is therefore reasonable to increase consumption of fatty sea fish, canola and flax seeds oil rich in n-3 family fatty acids by sportsmen.

ketogenic diet maybe to increase of total testosterone same in eugonadal man. Probably the pathway of total testosterone increase by the ketogenic diet is the parallel increase in cholesterol intake. However, it is still unknown a specific ratio among dietary cholesterol intake with total testosterone increase. Some attention points should be clarified, as if there is benefit of increased cholesterol intake and/or ketogenic diet in the increase of testosterone in men with hypogonadism and to what point would be biologically relevant (i.e. increased musculoskeletal weight and libido) this increase in eugonadal man.

In February 2015, the Dietary Guidelines Advisory Committee proposed to drop a longstanding recommendation that Americans restrict their dietary cholesterol intake because “available evidence shows no appreciable relationship between consumption of dietary cholesterol and serum cholesterol.” Although this holds for most healthy individuals, many studies have shown that the response to changes in dietary cholesterol is heterogeneous in humans and that cholesterol consumption should be restricted by diabetics and others at risk for cardiovascular disease. As eggs are among the most cholesterol-rich foods consumed by man, the production of low-cholesterol eggs remains a viable research goal and represents a desirable dietary option for persons who need to restrict their cholesterol intake but wish to include eggs in their diet. This chapter summarizes studies linking cholesterol consumption to cardiovascular disease risk and reviews approaches to reducing the cholesterol content of shell eggs.

This study aims to analyze the relation between fiber intake, cholesterol, nutritional status, and physical activity with blood cholesterol level on adult women. The study design was a cross sectional study. The study involved 60 adult women (aged 40-64 y.o) in the Sanggar Senam Bugar Sehat Bogor as respondent. The analysis used was Chi-square test to assess the relation between variables. The result showed a signinificant relation of physical activity (p value=0,027, OR=0,098 ; 95% CI=0,012-0,809), fiber intake (p value=0,046, OR=7,724 ; 95% CI=0,918-64,260), and cholesterol intake (p value=0,000, OR=16,00 ; 95% CI=3,398-75,345) with blood cholesterol level. It is concluded that blood cholesterol levels are influenced by eating pattern, physical activities, and routine medical check up.

The 2015 Dietary Guidelines for Americans recommends that individuals should minimize their dietary cholesterol intake. However, current dietary cholesterol intake and its food sources have not been well-characterized. We examined dietary cholesterol intake by age, sex, race, and food sources using 24-h dietary recall data from a nationally representative sample of 5047 adults aged 20 years or older who participated in NHANES (2013&ndash, 2014 survey cycle). We also reported trends in cholesterol intake across the past seven NHANES surveys. Mean dietary cholesterol intake was 293 mg/day (348 mg/day for men and 242 mg/day for women) in the 2013&ndash, 2014 survey cycle, 39% of adults had dietary cholesterol intake above 300 mg/day (46% for men and 28% for women). Meat, eggs, grain products, and milk were the highest four food sources of cholesterol, contributing to 96% of the total consumption. Both average cholesterol intake and food source varied by age, sex, and race (each p &lt, 0.05). Mean cholesterol intake of the overall population had been relatively constant at ~290 mg/day from 2001&ndash, 2002 to 2013&ndash, 2014 (p-trend = 0.98). These results should inform public health efforts in implementing dietary guidelines and tailoring dietary recommendations.

This study evaluated the diet of 615 professional athletes (PA) (306 males and 309 females; 21.3±5.5 years) compared with 312 age-matched non-athletes (NA), who participated in the 3 rd Hungarian National Dietary Survey in 2003. Dietary intake was assessed based on 3-day food record. In males, daily energy intake was 3513±1143 kcal and 2965±551 kcal for PA and NA, respectively (P

Dietary goals designed to improve nutritional health and lower the risk of chronic disease have been drawn up in Australia. Compliance with quantified goals was assessed in a random sample of 91 residents of Nambour, a typical community in southeast Queensland. Compliance was highest for alcohol and cholesterol goals. About three‐quarters of the sample complied with the goal of limiting alcohol intake to five per cent of energy intake, and over half complied with the goal to limit daily cholesterol intake to less than 300mg. Compliance was lower for total fat and fibre goals. About 40% of the sample complied with the goal of consuming less than 33% of energy as fat or the goal of consuming 30g or more fibre per day. Compliance was slightly better for women than for men. Only a small proportion of the sample complied with goals to increase total carbohydrate intake to 55% or more of energy, or to limit saturated fatty acid intake to 10% of energy. For each of the goals, some were far from complying. In general, compliance with goals was lowest for males under 40 and was also low for women under 40. 1992 Public Health Association of Australia

Summary Background & aims Low Zinc (Zn) and Magnesium (Mg) concentrations during pregnancy are implicated with low birth weight, malformations and increased mortality and morbidity. We aimed to compare serum concentrations of Zn and Mg in Greek (N = 219) and immigrant Albanian mothers (N = 230) at delivery and in the cord blood of their neonates. Methods A 60 day's food diary was kept from each mother. Zn and Mg sera concentrations were measured with atomic absorption spectrometry. Results Carbohydrates, total fat and cholesterol intake were significantly higher in Albanian mothers when compared with those of Greek origin. On the contrary, total protein and especially animal protein intake were higher in the Greek mothers. Magnesium intake was similar between the two ethnic groups, whereas Zn intake in Albanian mothers was evaluated lower (5.8 ± 1.4 mg/d) when compared with that of Greeks (10.8 mg ± 1.2 mg/d, p 0.05). In contrast, Zn concentration was significantly lower (49.8 ± 1.2 μg/dl) in the Albanian mothers as compared with that of Greeks (62.2 ± 1.4 μg/dl, p Conclusion Mg serum concentrations were similar in the studied mothers and their neonates. Zn serum concentrations were significantly lower in Albanian mothers and newborns than those of Greek origin, probably due to the reduced rich in Zn animal protein intake from Albanians. The latter may be the consequence of their low socioeconomic status.

We report a sensitive, generic method for quantitative profiling of bile acids and other endogenous metabolites in small quantities of various biological fluids and tissues. The method is based on a straightforward sample preparation, separation by reversed-phase high performance liquid-chromatography mass spectrometry (HPLC-MS) and electrospray ionisation in the negative ionisation mode (ESI-). Detection is performed in full scan using the linear ion trap Fourier transform mass spectrometer (LTQ-FTMS) generating data for many (endogenous) metabolites, not only bile acids. A validation of the method in urine, plasma and liver was performed for 17 bile acids including their taurine, sulfate and glycine conjugates. The method is linear in the 0.01-1 μM range. The accuracy in human plasma ranges from 74 to 113%, in human urine 77 to 104% and in mouse liver 79 to 140%. The precision ranges from 2 to 20% for pooled samples even in studies with large number of samples (n > 250). The method was successfully applied to a multi-compartmental APOE*3-Leiden mouse study, the main goal of which was to analyze the effect of increasing dietary cholesterol concentrations on hepatic cholesterol homeostasis and bile acid synthesis. Serum and liver samples from different treatment groups were profiled with the new method. Statistically significant differences between the diet groups were observed regarding total as well as individual bile acid concentrations. © 2008 Elsevier B.V. All rights reserved.

ObjectiveThe rate of type 2 diabetes mellitus among Inuit is 12·2 % in individuals over 50 years of age, similar to the Canadian prevalence. Given marked dietary transitions in the Arctic, we evaluated the dietary and other correlates of not previously diagnosed glucose intolerance, defined as type 2 diabetes mellitus, impaired fasting glucose or impaired glucose tolerance.DesignCross-sectional analyses were limited to adults with a completed 2 h oral glucose tolerance test and without pre-existing diabetes. Anthropometric assessments, health and medication usage questionnaires and a 24 h dietary recall were administered.SettingCanadian International Polar Year Inuit Health Survey (2007–2008).SubjectsInuit adults (n 777).ResultsGlucose intolerance was associated with older age and adiposity. Percentage of energy from protein above the Acceptable Macronutrient Distribution Range of 35 %, compared with intake within the range, was associated with increased odds of glucose intolerance (OR=1·98; 95 % CI 1·09, 3·61) in multivariable analyses. Further, cholesterol intake in the highest three quartiles combined (median exposures of 207, 416 and 778 mg/d, respectively) compared with the lowest quartile (median intake of 81 mg/d) was associated with glucose intolerance (OR=2·15; 95 % CI 1·23, 3·78) in multivariable analyses. Past-day traditional food consumption was borderline protective of glucose intolerance (P=0·054) and high fibre intake was not significantly protective (P=0·08).ConclusionsThe results contribute to the existing literature on high protein and cholesterol intakes as they may relate to diabetes risk.

The objective of this study was to assess the nutritional quality of Mexican men’s diet and its relation to socio-economic status (SES) and BMI. A random sample of 325 Mexican men, aged 35–65 y and stratified by SES and urban or rural residence, took part in the study. Two nonconsecutive 24-h dietary recalls were conducted in person. Dietary diversity was based on the consumption of 24 food groups. The micronutrient adequacy score that was used as a cut off was 75% of the U.S. RDA (Institute of Medicine) for 13 vitamins and minerals. A prevention score assessed subjects’ adherence to 8 WHO dietary recommendations for the prevention of chronic diseases. Dietary diversity and micronutrient adequacy increased with SES status of urban subjects; these indices were also significantly higher among urban poor than among rural poor. In contrast, the prevention score tended to be higher among the poorer urban and rural respondents. Dietary diversity was significantly higher but the preventive score was significantly lower in obese subjects than in normal BMI subjects. There was a positive correlation between diet diversity and micronutrient adequacy (r ¼ 0.34, P , 0.01), whereas dietary diversity and the prevention score were inversely correlated (r ¼ 20.21, P , 0.01). The inverse association of dietary diversity and the prevention score was stronger in rural than in urban subjects. Dietary diversity was positively and significantly correlated with fruit and vegetable intake (r ¼ 0.295, P , 0.001). It was, however, also positively correlated with the percent of energy from total and saturated fat, and cholesterol intake. Therefore, in some settings, higher food diversity may not predict a healthier diet from the standpoint of the prevention of chronic diseases. J. Nutr. 136: 2921–2927, 2006.

KEYWORDS - CLASSIFICATION: adverse effects;analysis;Boston;cancer epidemiology;Cholesterol;Cohort Studies;dietary modulation of cancer & cancer biomarkers;Dietary Fats;epidemiology;etiology;Eggs;Female;Food Habits;Humans;North America;Ovarian Neoplasms;Public Health;Research;Risk. Fat and cholesterol are theorized to promote ovarian carcinogenesis by increasing circulating estrogen levels. Although case-control studies have reported positive associations between total and saturated fat intake and ovarian cancer risk, two cohort studies have observed null associations. Dietary cholesterol and eggs have been positively associated with ovarian cancer risk. A pooled analysis was conducted on 12 cohort studies. Among 523,217 women, 2,132 incident epithelial ovarian cancer cases were identified. Study-specific relative risks (RR) and 95% confidence intervals (CI) were calculated by Cox proportional hazards models, and then pooled using a random effects model. Total fat intake was not associated with ovarian cancer risk (pooled multivariate RR = 1.08, 95% CI 0.86-1.34 comparing > or =45 to 30

Since the American Heart Association last presented nutrition guidelines for children, significant changes have occurred in the prevalence of cardiovascular risk factors and nutrition behaviors in children. Overweight has increased, whereas saturated fat and cholesterol intake have decreased, at least as percentage of total caloric intake. Better understanding of children’s cardiovascular risk status and current diet is available from national survey data. New research on the efficacy of diet intervention in children has been published. Also, increasing attention has been paid to the importance of nutrition early in life, including the fetal milieu. This scientific statement summarizes current available information on cardiovascular nutrition in children and makes recommendations for both primordial and primary prevention of cardiovascular disease beginning at a young age.

Background: The endogenous cholesterol fractional synthesis rate (FSR) is related inversely to infant dietary cholesterol at 4 months of age; however, it remains to be established whether this effect is permanent, possibly contributing to later hypercholesterolemia. Objective: To determine whether levels of dietary cholesterol in infancy induced changes in FSR and plasma lipid levels that persisted at 18 months. Methods. A prospective clinical trial was conducted with 47 infants, from their first week of life until 18 months of age, who received human milk (HM) until weaned (n = 15) or were randomized to receive modified cow’s milk formula (MCF) with added cholesterol (n = 15) or cow’s milk formula (CF) (n = 17) for 12 months. Cholesterol contents of HM, MCF, and CF were 120, 80, and 40 mg/L, respectively. FSR and plasma lipid levels were measured at 4 and 18 months. Results: At 4 months, total cholesterol and low-density lipoprotein cholesterol levels were higher for infants fed HM and MCF, compared with CF. High-density lipoprotein cholesterol levels were higher in the MCF group than in the HM and CF groups. FSR in the HM group (0.034 ± 0.005 pools per day) was lower than that in the CF group (0.052 ± 0.005 pools per day). There was no difference between the HM and MCF (0.047 ± 0.005 pools per day) groups or between the MCF and CF groups. At 18 months, there were no differences in FSRs or plasma lipid profiles between the groups. Conclusion: Although cholesterol intake before weaning affects FSRs and plasma lipid profiles at 4 months, these differences do not persist after weaning to an unrestricted diet at 18 months. This provides additional evidence that there is no imprinting of FSR in infancy with differing dietary levels of cholesterol. Pediatrics 2005;115:1594–1601; cholesterol, breastfed, formula-fed, fractional synthesis rate, deuterium., available, unlimited, public

The Finnish national food composition database Fineli®was updated with recent analytical values for plant sterols (PS) (sitosterol, campesterol, stigmasterol, avenasterol, brassicasterols and stanols) and cholesterol. The quality of the new analytical data was assessed. The aims of the present study were: (1) to compare the effect of old and new database values on PS and cholesterol intakes based on average per capita food consumption data; (2) to estimate the current intake and major sources of these compounds in various population groups according to the national FINDIET 1997 survey data. The intake of total PS was 305 mg/d for men and 237 mg/d for women. The respective intakes for cholesterol were 284 mg/d and 201 mg/d. Women had a higher density of PS in their diets than men, whereas the cholesterol density in the diets did not differ between genders. Cereals, margarine, vegetables and vegetable oils were the main food sources of PS. Meat, meat products and eggs were the main sources of cholesterol. A 9 % greater PS intake estimate was obtained with the new PS database compared with the old PS database, probably due to minor methodological differences between the new and old analyses. Notable changes in analytical methods suggest a lower value (−19 %) for cholesterol intake calculated from the new database compared with the old one. We conclude that researchers can have confidence in the new values for PS and cholesterol, because systematic evaluation of the new analytical values showed them to be of high quality.

Objective: We aimed to study the long-term associations between sucrose intake (SI), selected representatives of the cariogenic oral flora, and the dental health of children from 3 to 16 years of age. Methods: At 7 months of age 1,062 infants (540 intervention; 522 controls) were included in the prospective, randomised STRIP-project aimed at restricting the child's saturated fat and cholesterol intake to prevent atherosclerosis when they become adults. At 3 years of age, every fifth child was invited (n = 178) to an oral sub-study, and 148 (78 boys) children attended. A restudy was conducted on 135 children aged 6, 127 aged 9, 114 aged 12 and 88 aged 16. SI using 4-day food records, plate-cultured mutans streptococci (MS), salivary lactobacilli (LB) and yeasts using commercial kits (Orion Diagnostica, Espoo Finland), toothbrushing frequency using fluoridated toothpaste and dental health expressed as d3mft/D3MFT were regularly recorded. Results: The SI of children whose intake was ≥10 E% (high SI) at 3 years remained high throughout the entire follow-up (p < 0.001, GLM for repeated measures) period, and they had higher salivary MS and LB counts (p = 0.024 and p = 0.068, respectively, GLM) than their counterparts whose SI was below 10 E% (low SI). No differences in toothbrushing habits were found between the high and low SI-groups. Caries-survival was strongly associated with low 6-year-counts of MS (p = 0.008, Cox regression analysis), and the d3mft/D3MFT-scores of the high SI-group were higher than those of the low SI-group (p = 0.046, GLM). Conclusions: High SI at 3 years was associated with high MS-counts (≥105 cfu/ml) and with a high risk for caries.

Low-density lipoprotein receptor-related protein 5 (LRP5) is a member of the LDLR family that orchestrates cholesterol homoeostasis. The role of LRP5 and the canonical Wnt pathway in the vascular wall of dyslipidaemic animals remains unknown. In this study, we analysed the role of LRP5 and the Wnt signalling pathway in mice fed a hypercholesterolaemic diet (HC) to trigger dyslipidaemia. We show that Lrp5-/- mice had larger aortic lipid infiltrations than wild-type mice, indicating a protective role for LRP5 in the vascular wall. Three members of the LDLR family, Lrp1, Vldlr and Lrp6, showed up-regulated gene expression levels in aortas of Lrp5-/- mice fed a hypercholesterolaemic diet. HC feeding in Lrp5-/- mice induced higher macrophage infiltration in the aortas and accumulation of inflammatory cytokines in blood. Wnt/ß-CATENIN signalling proteins were down-regulated in HC Lrp5-/- mice indicating that LRP5 regulates the activation of Wnt signalling in the vascular wall. In conclusion, our findings show that LRP5 and the canonical Wnt pathway down-regulation regulate the dyslipidaemic profile by promoting lipid and macrophage retention in the vessel wall and increasing leucocyte-driven systemic inflammation. © 2015 The Authors.