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Obesity is associated with autoimmunity, a phenomenon considered as harmful. Here we show that obese mice and humans produce IgG-type autoantibodies that specifically recognize apolipoprotein B-100 (ApoB100), its native epitope p210, and the synthetic p210 mimotope pB1. By contrast, antibodies against epitopes p45 and p240, which have been associated with atherosclerosis, were not detected in either the humans or mice. In a longitudinal analysis of high fat diet-fed mice, autoantibody production rose with increasing body weight, then decreased and plateaued at morbid obesity. Likewise, in a cross-sectional analysis of sera from 148 human volunteers spanning a wide BMI range and free of comorbidities, the immunoreactivity increased and then decreased with increasing BMI. Thus, the obesity-related ApoB100-specific natural autoantibodies characteristically showed the same epitope recognition, IgG-type, and biphasic serum levels in humans and mice. We previously reported that a pB1-based vaccine induces similar antibodies and can prevent obesity in mice. Therefore, our present results suggest that autoantibodies directed against native ApoB100 may mitigate obesity, and that the vaccination approach may be effective in humans.

Fibromyalgia (FM), chronic widespread pain, exhibits spontaneous pain without external stimuli and is associated with altered brain activities during resting state. To understand the topological features of brain network in FM, we employed persistent homology which is a multiple scale network modeling framework not requiring thresholding. Spontaneous magnetoencephalography (MEG) activity was recorded in 19 healthy controls (HCs) and 18 FM patients. Barcode, single linkage dendrogram and single linkage matrix were generated based on the proposed modeling framework. In theta band, the slope of decrease in the number of connected components in barcodes showed steeper in HC, suggesting FM patients had decreased global connectivity. FM patients had reduced connectivity within default mode network, between middle/inferior temporal gyrus and visual cortex. The longer pain duration was correlated with reduced connectivity between inferior temporal gyrus and visual cortex. Our findings demonstrated that the aberrant resting state network could be associated with dysfunction of sensory processing in chronic pain. The spontaneous nature of FM pain may accrue to disruption of resting state network.

Background: Recently, a peptide vaccine (B4T) was developed that prevents high fat diet (HFD)-induced obesity and liver steatosis in wild type mice and appears to target an epitope present in ApoB100 but not ApoB48. Here, we ask whether B4T remains effective in ApoE knockout (ApoE-ko) mice, which exhibit a greatly increased ApoB48/ApoB100 ratio and develop atherosclerosis under HFD., Methods: HFD-fed male ApoE-ko mice were injected with B4T or vehicle 3 times between 5 and 15 weeks of age. Until 45 weeks of age, they were regularly weighed and antibody titers determined. In the end, adiposity and organ histologies were examined., Results: We find that in the ApoE-ko mice, B4T prevents HFD-induced body weight increases (p<0.01) to a comparable degree as previously shown in wild type mice. Also, liver steatosis was prevented as previously shown in wild type mice. By contrast, atherosclerotic plaque formation was not prevented in any of the vaccinated mice studied, in line with the observation that antibody production paralleled the weight reduction but largely preceded atherogenesis., Conclusion: The findings demonstrate effectiveness of B4T despite the increased ApoB48/B100 ratio, but argue against an effect on de novo plaque formation. At least under the current vaccination schedule, the obesity- and atherosclerosis-related roles of ApoB appear to be dissociable., (Copyright © 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)

Background: The generation of induced pluripotent stem cell (iPSC), a substitute for embryonic stem cell (ESC), requires the proper orchestration of a transcription program at the chromatin level. Our recent approach for the induction of pluripotent stem cells from fibroblasts using protein extracts from mouse ESCs could overcome the potential tumorigenicity risks associated with random retroviral integration. Here, we examine the epigenetic modifications and the transcriptome of two types of iPSC and of partially reprogrammed iPSCs (iPSCp) generated independently from adult cardiac and skin fibroblasts to assess any perturbations of the transcription program during reprogramming., Results: The comparative dissection of the transcription profiles and histone modification patterns at lysines 4 and 27 of histone H3 of the iPSC, iPSCp, ESC, and somatic cells revealed that the iPSC was almost completely comparable to the ESC, regardless of their origins, whereas the genes of the iPSCp were dysregulated to a larger extent. Regardless of the origins of the somatic cells, the fibroblasts induced using the ESC protein extracts appear to be completely reprogrammed into pluripotent cells, although they show unshared marginal differences in their gene expression programs, which may not affect the maintenance of stemness. A comparative investigation of the iPSCp generated by unwanted reprogramming showed that the two groups of genes on the pathway from somatic cells to iPSC might function as sequential reprogramming-competent early and late responders to the induction stimulus. Moreover, some of the divergent genes expressed only in the iPSCp were associated with many tumor-related pathways., Conclusions: Faithful transcriptional reprogramming should follow epigenetic alterations to generate induced pluripotent stem cells from somatic cells. This genome-wide comparison enabled us to define the early and late responder genes during the cell reprogramming process to iPSC. Our results indicate that the cellular responsiveness to external stimuli should be pre-determined and sequentially orchestrated through the tight modulation of the chromatin environment during cell reprogramming to prevent unexpected reprogramming., Competing Interests: The authors have declared that no competing interests exist.

Beginning in 2000, in economically advanced countries, a remarkable bifurcation in fertility levels has emerged, with one group in the moderate range of period total fertility rates (TFR), about 1.9, and the other at 1.3. The upper branch consists of countries in Northern and Western Europe, Oceania and the United States; the lower branch includes Central, Southern and Eastern Europe, and East and Southeast Asia. A review of the major theories for low fertility countries reveals that none of them would have predicted this specific bifurcation. We argue that those countries with fertility levels close to replacement level have institutional arrangements, and related policies, that make it easier, not easy, for women to combine the worker and mother roles. The institutional details are quite different across countries, suggesting that multiple combinations of institutional arrangements and policies can lead to the same country-level fertility outcome. Canada, the only exception to this bifurcation, illustrates the importance of the different institutional structures in Québec compared to the rest of Canada.

Using discrete-time survival models of parity progression and illustrative data from the Philippines, this article develops a multivariate multidimensional life table of nuptiality and fertility, the dimensions of which are age, parity, and duration in parity. The measures calculated from this life table include total fertility rate (TFR), [corrected] total marital fertility rate (TMFR), parity progression ratios (PPR), age-specific fertility rates, mean and median ages at first marriage, mean and median closed birth intervals, and mean and median ages at childbearing by child's birth order and for all birth orders combined. These measures are referred to collectively as "TFR and its components." Because the multidimensional life table is multivariate, all measures derived from it are also multivariate in the sense that they can be tabulated by categories or selected values of one socioeconomic variable while controlling for other socioeconomic variables. The methodology is applied to birth history data, in the form of actual birth histories from a fertility survey or reconstructed birth histories derived from a census or household survey. The methodology yields period estimates as well as cohort estimates of the aforementioned measures.

The paper examines changes in the relationship between employment and household tasks of Japanese couples, using data drawn from national cross-sectional surveys in 1994, 2000 and 2009 of persons aged 20-49 and from the 2009 follow-up of the 2000 survey. Wives' employment is structured by their husbands' employment time and earning power, as well as by their family situations including the presence and age of children and coresidence with parents. Housework hours of husbands, though very low, increased over time, while wives' hours decreased. Wives housework time decreases as their employment time increases. Marriage dramatically increases women's housework time but produces little change in men's time. Husbands' housework hours are positively correlated with reported marital satisfaction of both spouses.

Chromatin structure and dynamics that are influenced by epigenetic marks, such as histone modification and DNA methylation, play a crucial role in modulating gene transcription. To understand the relationship between histone modifications and regulatory elements in breast cancer cells, we compared our chromatin immunoprecipitation sequencing (ChIP-Seq) histone modification patterns for histone H3K4me1, H3K4me3, H3K9/16ac, and H3K27me3 in MCF-7 cells with publicly available formaldehyde-assisted isolation of regulatory elements (FAIRE)-chip signals in human chromosomes 8, 11, and 12, identified by a method called FAIRE. Active regulatory elements defined by FAIRE were highly associated with active histone modifications, like H3K4me3 and H3K9/16ac, especially near transcription start sites. The H3K9/16ac-enriched genes that overlapped with FAIRE signals (FAIRE-H3K9/14ac) were moderately correlated with gene expression levels. We also identified functional sequence motifs at H3K4me1-enriched FAIRE sites upstream of putative promoters, suggesting that regulatory elements could be associated with H3K4me1 to be regarded as distal regulatory elements. Our results might provide an insight into epigenetic regulatory mechanisms explaining the association of histone modifications with open chromatin structure in breast cancer cells.

Histone modifications are regarded as one of markers to identify regulatory elements which are DNA segments modulating gene transcription. Aberrant changes of histone modification levels are frequently observed in cancer. We have employed ChIP-Seq to identify regulatory elements in human breast cancer cell line, MCF-7 by comparing histone modification patterns of H3K4me1, H3K4me3, and H3K9/14ac to those in normal mammary epithelial cell line, MCF-10A. The genome-wide analysis shows that H3K4me3 and H3K9/14ac are highly enriched at promoter regions and H3K4me1 has a relatively broad distribution over proximity of TSSs as well as other genomic regions. We identified that many differentially expressed genes in MCF-7 have divergent histone modification patterns. To understand the functional roles of distinctively histone-modified regions, we selected 35 genomic regions marked by at least one histone modification and located from 3 to 10 kb upstream of TSS in both MCF-7 and MCF-10A and assessed their transcriptional activities. About 66% and 60% of selected regions in MCF-7 and MCF-10A, respectively, enhanced the transcriptional activity. Interestingly, most regions marked by H3K4me1 exhibited an enhancer activity. Regions with two or more kinds of histone modifications did show varying activities. In conclusion, our data reflects that comprehensive analysis of histone modification profiles under cell type-specific chromatin environment should provide a better chance for defining functional regulatory elements in the genome., (Copyright © 2012 Elsevier Inc. All rights reserved.)

Substantial family and work macro-level change has been occurring in Japan. Examples include a decline in the availability of jobs that afford lifetime protection against unemployment, an increase in jobs that do not carry benefits such as a pension, an increase in age at marriage and at first birth, and an increase in marital dissolution. Using life history data from the 2000 National Survey on Family and Economic Conditions, young Japanese appear to have responded to these macro-level changes in a fairly orderly manner. Marriage and childbearing have been postponed, but marriage still precedes childbearing. Education is completed prior to starting work. For men, once work commences they continue working. For women, the classic conflict between work and family roles is evident. For men and women in both the family and work spheres Japanese young adults have more orderly life course trajectories than American young adults.

An analysis of data mainly from China's 1990 and 2000 censuses and 2005 mini-census shows how fertility decline between 1975 and 2005 in the province of Guangdong has been influenced by both fertility policy and economic and social development. Guangdong's development since 1975 has been very rapid and has attracted huge numbers of migrants from other provinces. The analysis of the province's fertility trend from 1975 shows clearly the influence of fertility policy on the trend. The analysis also shows that economic development has brought about large changes in population composition by urban/rural residence, education, occupation, and migration status, which, together with large fertility differentials by these characteristics, have contributed substantially to Guangdong's fertility decline, in large part through changes in proportions currently married.

This paper assesses the effects of individual- and community-level knowledge of HIV transmission, beliefs and fear of infection on individual attitudes toward and willingness to interact with people living with HIV/AIDS in China. Data are from a sample survey of 12,270 men and women age 15-49 from seven provinces/municipalities. The survey was conducted by the Population and Family Planning Commission in December 2003. Multilevel regression analyses show that stigmatization of people living with HIV/AIDS is strongly associated with community-level HIV knowledge and fear, even after taking into account HIV knowledge and fear at the individual level. The findings suggest that individual attitudes are partially shaped through social learning and social influence. Public-health interventions aimed at eliminating stigmatization of people living with HIV/AIDS will need to pay special attention to social and community influences while continuously working to enhance HIV knowledge and reduce inaccurate beliefs and fear.

Objective: To assess the effect of Nepal's vitamin A supplementation programme on child mortality at age 12-59 months., Materials and Methods: Logistic regression, applied to retrospective data from Nepal's 2001 Demographic and Health Survey., Results: After a number of potentially confounding variables are controlled, the effect of 100% community-level vitamin A coverage since the child's birth, relative to no coverage, is to reduce the odds of dying at age 12-59 months by slightly more than half (OR = 0.47, P = 0.03)., Conclusions: The estimated beneficial effect of vitamin A supplementation on child mortality is larger than that found in most earlier clinical studies. This larger effect may be due mainly to the other health-related activities undertaken by the female community health volunteers who distribute vitamin A capsules.

This paper examines age patterns of first marriage and motherhood and covariates of early marriage, delayed consummation of marriage and early motherhood in Nepal using data from the 2000 Nepal Adolescent and Young Adult Survey (NAYA). Both unmarried and married male and female youths (age 14-22) were included in the survey. The analysis is based on 2800 urban youths and 5075 rural youths with complete information on the variables examined. Proportional hazard models are used to estimate covariates of early marriage and early motherhood, and logistic regression models are used to estimate covariates of delayed consummation of marriage. The results show that early marriage and early motherhood are quite common among Nepalese women, especially in rural areas. Early marriage is much less common among men. Delayed consummation of marriage is common among very young brides, especially in rural areas. The main covariates associated with early marriage and early motherhood are respondent's education, region of residence and ethnicity. The main covariates of delayed consummation of marriage are age at first marriage, region of residence and ethnicity. The study highlights the need to focus on less educated female youths in the Terai region in order to reduce the reproductive and child health risks associated with early marriage and early childbearing.

This article examines the contextual effects of community environment on individual stigmatizing attitudes toward people with HIV/AIDS in China. Multilevel logistic regression models are used to analyze data on 5,658 respondents aged 15-49 from 66 communities in the Baseline Information, Education, and Communication Survey for HIV/AIDS Prevention in China, conducted by the State Family Planning Commission in 2000. The results show that a high level of HIV/AIDS-related risk behavior in the community and a low level of community development are associated with increased HIV/AIDS-related stigma, after controlling for respondents' sociodemographic characteristics, including extent of knowledge about HIV/AIDS. The findings suggest that interventions for reducing HIV/AIDS-related stigma in China should take into account community characteristics, such as level of HIV/AIDS-related risk behavior and level of development in the community.

In a national sample of high school students, 43 per cent had drunk regularly, 32 per cent had smoked regularly, and 11 per cent had experienced sexual intercourse. Boys were more likely to have experienced these behaviors than girls. Going to school in Seoul, not having two parents in the family, and having an experience of living away from immediate family were associated with higher livelihood of experiencing these behaviors. Students who had friends with sexual experiences were much more likely to experience sex themselves than those without., (Copyright 2001 The Association for Professionals in Services for Adolescents.)

In this paper we introduce a mixture model in which we combine logistic regression and piecewise proportional hazards models for analysis of duration data. The model allows simultaneous estimation of two sets of effects of covariates: one of the probability of an event and the other of the timing of the event. We illustrate the application of the model through an analysis of the effects of women's characteristics and of the acceptance of a one-child certificate on the birth of second children in China. Both factors affect the probability of having a second child, but only the acceptance of a one-child certificate has a significant and strong effect on the second-birth interval.

Using data from the 1988 Two-Per-Thousand Survey of Fertility and Birth Control, this paper examines the effects of gender, birth order, and other correlates of childhood mortality in China. Controlling for family-level factors, childhood mortality is found to be associated with the child's gender and birth order. Among firstborn children the difference between male and female childhood mortality is not statistically significant, but among others, female children between ages 1 and 5 experience higher mortality than male children. Childhood mortality is slightly higher for children who have older brothers only than for those who have older sisters only, and it is highest for those who have both older brothers and sisters. Other factors affecting childhood mortality in China include mortality of older siblings, birth interval, urban/rural residence, mother's level of education, and mother's occupation. All interactive effects between gender and family-level characteristics are found to be statistically insignificant.

This study focuses on the relationship between contraceptive behavior, family size preferences, and perceptions of the one-child policy among young Chinese women in rural areas of Jilin Province. In 1985, about 85 percent of rural married women with one surviving child were practicing contraception, although most of them reported two as their ideal number of children. Most women with one surviving child, including those with one-child certificates, were practicing contraception in response to the government campaign, while more than half of women with two or more children were doing so voluntarily. Most of the women with one child were using the IUD, whereas more than half of women with two or more children were sterilized. Through multivariate analysis of contraceptive behavior and method choice, additional factors were found to be associated with the contraceptive behavior of rural Jilin women; achievement of their ideal family size was a significant factor in the voluntary practice of contraception as well as in contraceptive method choice. Implications of the results are discussed.

"This paper examines how the level of child mortality and rate of out-migration, and their covariates were affected during the 1974 famine and the periods immediately following the famine in Bangladesh." The authors examine several variables affecting mortality and out-migration, including socioeconomic status, sex factors, age factors, educational status, and religion. Data are from the Matlab Demographic Surveillance System., (excerpt)

This paper studies the effect of infant death on subsequent fertility in a developing country, examining spacing of children and additional births following the survival or death of the preceding infant. The material is provided by the 1971 National Fertility Survey of Korea, 23,635 retrospective birth records of 6,285 women. The findings suggest that prior to the introduction of a national family planning program, the influence of infant death was limited to the biological effect resulting from a shortened lactational period. Since fertility regulation methods have been made available throughout the country, motivational effects to replace the lost child appeared to emerge. The proportion of excess births attributable to infant deaths has increased in recent years. However, because of the lowered infant mortality, the overall impact of infant death on the national fertility level appears to be small.

Contraceptive use and method mix were analysed using Philippines national survey data of 1973, 1978, and 1983. The analyses suggest that the reported decline in contraceptive prevalence between 1978 and 1983 was due to under-reporting of use in 1973 and 1983. The shifts in contraceptive method mix were also partly from under-reporting of rhythm and other methods in 1973 and 1983. Nevertheless, the determinants of method choice were similar in all three sets of data. Filipino couples were making rational choices in terms of their contraceptive goals, access, evaluation, and competence. Modifications in the directions and magnitude of the relationships in determining method choice also occurred, partly reflecting the increased use of sterilization by older, higher parity women.

Infant mortality trends and differentials are estimated from the 1981 Nepal Contraceptive Prevalence Survey (NCPS) and compared with similar estimates from the 1976 Nepal Fertility Survey (NFS) and the 1981 Census of Nepal. The analysis indicates that infant mortality rates derived directly from the NFS maternity histories are the most accurate. Infant mortality rates derived directly from the NCPS maternity histories are severely underestimated and yield a strongly biased trend that is the reverse of the true downward trend. Indirect estimates of infant mortality trends derived from child survivorship data do not result in a consistent pattern. Infant mortality differentials, when expressed in relative rather than absolute terms, are generally consistent with findings from earlier studies. Possible reasons for data quality differences among the three data sources are discussed.

The consistency of retrospective and current status data on contraceptive use from a series of national fertility surveys carried out during the 1970s in Korea is investigated. Aggregate consistency is examined among random samples from the same cohort or cohorts of women interviewed in each survey. The results indicate that estimates of trends in contraceptive use from a retrospective history in one survey, or from cross-sectional estimates in a series of surveys, can each yield misleading findings. Data from the 1974 Korean National Fertility Survey (KNFS) appear to be more reliable than those from other surveys, possibly because an interval-by-interval contraceptive history was used, explicit definitions of contraceptive methods were given prior to taking the contraceptive history, and the KNFS involved longer interviewer training and, perhaps, less time pressure during interviews.

"This paper presents some results from the 1986 Family Life Survey of Korea. This survey was the first that was conducted in Korea with the specific purpose of understanding women's life cycle beyond marriage and fertility....A probability sample of 3,400 households representing the whole country were selected, [including] interviews of ever-married women of ages 15 to 64.... The survey collected information on household composition, with basic characteristics of members of household including health status....marriage history, including how husbands were selected, pregnancy history including the age of children when they first left home and reason for leaving home, contraceptive use, health, and women's employment at different stages of life." (SUMMARY IN KOR), (excerpt)

This paper investigates the association of early childhood mortality (between birth and second birthday) with ethnicity in Nepal, based on data from the 1976 Nepal Fertility Survey, which was part of the World Fertility Survey. The approach is through a series of hazard models, which incorporate ethnicity, year of birth, mother's illiteracy, father's illiteracy, rural-urban residence, region, sex, maternal age, survival of previous birth, previous birth interval, and breast-feeding as covariates. Ethnic differentials in early childhood mortality are not explained by the other socioeconomic and demographic covariates, except for a modest effect of illiteracy, but the remaining covariates explain a great deal of variability in early childhood mortality itself. Analysis using an improved specification of breast-feeding as an age-varying covariate indicates, on average, that breast-feeding, relative to not breast-feeding, reduces age-specific mortality risks during the first 2 years of life by 76%, a very large effect.

This article shows that in Nepal breastfeeding almost completely explains the effects of following birth interval on childhood mortality during the first 18 months of age and partially explains the effect of following birth interval on childhood mortality between 18 and 60 months of age. Breastfeeding does not explain the effect of preceding birth interval on childhood mortality. The analysis is based on application of hazard models to data from the 1976 Nepal Fertility Survey.