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1,239 results on '"Choe, Han"'

33. Activation of TMEM16E scramblase induces ligand independent growth factor receptor signaling and macropinocytosis for membrane repair.

Author

Kim, Jung-Eun, Ko, Woori, Jin, Siwoo, Woo, Jin-Nyeong, Jung, Yuna, Bae, Inah, Choe, Han-Kyoung, Seo, Daeha, Hille, Bertil, and Suh, Byung-Chang

Subjects
EPIDERMAL growth factor receptors, LIFE sciences, CYTOLOGY, PINOCYTOSIS, MUSCULAR dystrophy, ANNEXINS
Abstract

The calcium-dependent phospholipid scramblase TMEM16E mediates ion transport and lipid translocation across the plasma membrane. TMEM16E also contributes to protection of membrane structure by facilitating cellular repair signaling. Our research reveals that TMEM16E activation promotes macropinocytosis, essential for maintaining plasma membrane integrity. This scramblase externalizes phosphatidylserine, typically linked to resting growth factor receptors. We demonstrate that TMEM16E can interact with and signal through growth factor receptors, including epidermal growth factor receptor, even without ligands. This interaction stimulates downstream phosphoinositide 3-kinase and facilitates macropinocytosis and internalization of annexin V bound to the membrane, a process sensitive to amiloride inhibition. Although TMEM16E is internalized during this process, it returns to the plasma membrane. TMEM16E- driven macropinocytosis is proposed to restore membrane integrity after perturbation, potentially explaining pathologies in conditions like muscular dystrophies, where TMEM16E functionality is compromised, highlighting its critical role in muscle cell survival. TMEM16E is a calcium-dependent scramblase that mediates ion transport and lipid translocation, contributing to membrane protection. This study shows TMEM16E promotes macropinocytosis, crucial for maintaining plasma membrane integrity. [ABSTRACT FROM AUTHOR]

Published
2025
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44. One-step optogenetics with multifunctional flexible polymer fibers

Author

Park, Seongjun, Guo, Yuanyuan, Jia, Xiaoting, Choe, Han Kyoung, Grena, Benjamin, Kang, Jeewoo, Park, Jiyeon, Lu, Chi, Canales, Andres, Chen, Ritchie, Yim, Yeong Shin, Choi, Gloria B, Fink, Yoel, and Anikeeva, Polina

Subjects
Biotechnology, Neurosciences, Animals, Basolateral Nuclear Complex, Brain, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Channelrhodopsins, Electrodes, Implanted, Evoked Potentials, Hippocampus, Male, Mice, Mice, Transgenic, Motor Activity, Neural Pathways, Neurons, Opsins, Optical Fibers, Optogenetics, Photic Stimulation, Polymers, Prefrontal Cortex, Psychology, Cognitive Sciences, Neurology & Neurosurgery
Abstract

Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities in a single biocompatible platform remains a challenge. We developed a device composed of an optical waveguide, six electrodes and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes while providing collocated neural recording and optical stimulation. The miniature (

Published
2017

45. TiO2coatings doped with MoS2nanoparticles using plasma electrolytic oxidation on Ti–6Al–4V alloy: Application for enhanced and functional bio-implant surface

Author

Nisar, Sidra Sadaf and Choe, Han-Cheol

Abstract

The focus of this study was to examine the surface, mechanical, corrosion, and bioactive characteristics of TiO2coatings doped with different grams of nanoparticles of MoS2, which were fabricated using the plasma electrolytic oxidation (PEO) technique, and the structure, morphology, surface characteristics, and corrosion resistance of MoS2and TiO2coatings were assessed. The coatings were fabricated on the Ti-6Al-4V alloy substrate using a PEO setup, with varying concentrations of MoS2nanoparticles incorporated into the electrolyte solution. FESEM, XRD, and AFM were utilized to analyze the surface characteristics of the coatings. Also, PDP and AC impedance tests were done to check corrosion properties. Raman analysis, XPS, and cell tests were also done. The findings demonstrated that incorporating MoS2nanoparticles resulted in creating a composite coating characterized by adequately increased surface energy, wettability, and improved adhesion to the substrate. The FESEM analysis revealed a uniform dispersion of MoS2throughout the TiO2matrix forming a homogeneous and compact coating structure and also doping inside the PEO pores. XRD analysis confirmed the presence of TiO2, and the addition of MoS2nanoparticles further improved the crystallinity of the TiO2coating. AFM measurements demonstrated a slight increase in surface roughness and an increase in surface hardness upon the inclusion of MoS2. Compared to pure TiO2, the TiO2coating doped with MoS2displayed superior electrochemical corrosion resistance and cell proliferation properties. Alazirin staining, ALP, and mRNA activity showed that lower MoS2content such as P–2MoS2to P–6MoS2showed better cellular differentiation properties.

Published
2024
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