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7. Development of 3D Printable Calcium Phosphate Cement Scaffolds with Cockle Shell Powders.

Author

Cho, Eunbee, Kim, Jae Eun, Lee, Juo, Park, Sangbae, Lee, Sungmin, Chung, Jong Hoon, Kim, Jungsil, and Seonwoo, Hoon

Subjects
*CALCIUM phosphate, *CALCIUM compounds, *CEMENT, *BONE regeneration, *HYALURONIC acid, *MARINE natural products, *PRINT materials
Abstract

Three-dimensional (3D) printed calcium phosphate cement (CPC) scaffolds are increasingly being used for bone tissue repair. Traditional materials used for CPC scaffolds, such as bovine and porcine bone, generally contain low amounts of calcium phosphate compounds, resulting in reduced production rates of CPC scaffolds. On the other hand, cockle shells contain more than 99% CaCO3 in the form of amorphous aragonite with excellent biocompatibility, which is expected to increase the CPC production rate. In this study, 3D-printed cockle shell powder-based CPC (CSP-CPC) scaffolds were developed by the material extrusion method. Lactic acid and hyaluronic acid were used to promote the printability. The characterization of CSP-CPC scaffolds was performed using Fourier transform infrared spectra, X-ray diffraction patterns, and scanning electron microscopy. The biocompatibility of CSP-CPC scaffolds was evaluated using cell viability, Live/Dead, and alkaline phosphatase assays. In addition, CSP-CPC scaffolds were implanted into the mouse calvarial defect model to confirm bone regeneration. This study provides an opportunity to create high value added in fishing villages by recycling natural products from marine waste. [ABSTRACT FROM AUTHOR]

Published
2023
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