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Purpose: Interleukin (IL)-19 was expressed in invasive ductal carcinoma (IDC) of the breast tissue but not in healthy breast tissue. We explored the effects of IL-19 on the pathogenesis of breast cancer and its clinical outcome.Experimental Design: Tumor expression of IL-19 was assessed by immunohistochemistry and/or real-time quantitative PCR between two groups of patients with breast IDC (n = 60 and 143, respectively) with available clinical and survival data. We examined the effects of IL-19 on cytokine and chemokine production as well as proliferation and migration in breast cancer cells. Mice were injected with IL-19–overexpressing or vector control 67NR cells and the tumor growth and lung metastatic micronodules were measured.Results: Of the IDC specimens, high IL-19 expression was associated with advanced tumor stage, high tumor metastasis, and worse survival. In vitro, IL-19 induced transcripts of IL-1β, IL-6, TGF-β, matrix metalloproteinase (MMP)2, MMP9, and CXCR4 in 4T1 breast cancer cells; induced fibronectin expression and assembly; and promoted cancer cell proliferation and migration, which were inhibited by anti-IL-19 monoclonal antibody (mAb). Endogenous fibronectin expression and cancer cell migration were lower in IL-19 knockdown 4T1 cells. In 4T1 cells, hypoxia induced IL-19 and CXCR4 expression, which was inhibited by anti-IL-19 mAb. IL-19 overexpression in noninvasive 67NR cancer cells increased cell proliferation and migration. In vivo, mice injected with IL-19–overexpressing 67NR cell clones showed larger tumors and more metastatic micronodules in the lung.Conclusions: High IL-19 expression in breast cancer tissue is associated with a poor clinical outcome. IL-19 is pivotal in the pathogenesis of breast cancer. Clin Cancer Res; 18(3); 713–25. ©2011 AACR.

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Geographical Indications (GIs) can increase producer margins and contribute to local economic development, but the extent to which they do so depends on the nature of consumer demand. A Discrete Choice Experiment (DCE) considers the value that consumers place on a Protected Geographical Indication (PGI) in comparison with a leading manufacturer’s brand, as well as the importance of taste variations. Based on an application of DCE to sausages in Hungary, results indicate that a PGI can generate value to consumers exceeding that conveyed by the leading manufacturer’s brand. Consumers’ taste preferences, however, may not be consistent with the specification of GI products. Latent Class (LC) and Random parameter Latent Class (RLC) analyses identify two consumer segments, with the majority of consumers (71%-LC, 65%-RLC) classified as traditionalists, who most value the GI label, while a minority (29%-LC, 35%-RLC) is brand conscious, for whom the GI status is less salient. Both theoretical and business implications for GI marketing and club branding are drawn.

Purpose The purpose of this paper is to investigate the problem faced by many Taiwanese restaurant owners who trying to set up their shops in Thailand. Design/methodology/approach Two surveys were conducted in this study. The first interview questionnaire was designed using the factors proposed by Parasuraman et al. (1988, 1991) and given to restaurant owners/managers who successfully set up shops in Thailand. The second questionnaire was constructed specifically for Thai consumers. Findings Gray relational analysis (GRA), theory of inventive problem-solving (teoriya resheniya izobreatatelskikh zadatch, TRIZ) and interpretive structural model (ISM) were used to identify potential difficulties and to determine the key factors impacting the shop establishment and development in Thailand. The results provide a set of strategic sequence when launching restaurant in Thailand. Originality/value A result of GRA determined 14 important factors that influence customer perception of quality service. A TRIZ analytic process provided 17 strategies when setting up overseas shop and the ISM class diagram shown the three phases needed to be considered before restaurant owners can set up shops abroad. The three phases are construction, operation and competition phases. These set of strategies sequence when launching a restaurant in Thailand.

Consumers’ food purchase decision is multifaceted and complex, which is not only influenced by product and process characteristics, but also by the present decision-making circumstances. This study investigates this complexity of consumers’ food choice influenced by product and process characteristics, as well as by the decision-making circumstances (i.e. tactile sensory aspects of the product and shopping location), using best-worst scaling (BWS) methodology. BWS allows measuring the importance of different food buying criteria, thereby eliciting consumers’ preferences. A total of 795 food consumers participated in a street-intercept interview performed in three cities of Taiwan. Results indicate that among the nine food purchase criteria (e.g. country-of-origin (CoO) labelling, production methods, chemical residue testing (CRT) information, price, shopping location, visual appearance, sense of touch, package size, and a recommendation given by a significant one), CRT information is the most important criteria in consumers’ purchase decision, followed by CoO, production methods and hedonic characteristics. Three segments of consumers can be distinguished: the health-conscious purchasers (50.20%), the hedonic buyers (31.90%), and the origin-driven shoppers (17.90%).

This study investigates the relevance of psychological constructs in determining consumer intention to buy and Willingness-To-Pay (WTP) for a processed meat product, cured ham, differentiated by the attributes of animal welfare, ham variety, and price. Data obtained from an online survey conducted in Germany was used to estimate an integrated choice and latent variable (ICLV) model, which is based on an extension of the Theory of Planned Behavior (TPB) framework. There are two consumer segments that are identified: one that is highly price sensitive in its product choice and one that gives roughly equal weight to the animal welfare, ham variety, and price attributes. The ICLV model shows consistency across the two groups regarding the importance of psychological constructs—moral norms, attitude, and perceived behavioral control—in explaining respondent intentions to buy cured ham and their stated product choice. Subjective norms, however, are only a significant determinant of consumer intention to buy cured ham for the price sensitive consumer group.

International audience; This study aims at examining the consumers’ preferences and drivers affecting the choice of quality-labelled food products, i.e., protected designation of origin (PDO) labelled cheese. We applied the theory of planned behaviour (TPB) to analyse the purchase of Parmigiano Reggiano PDO and Comté PDO hard cheeses in Italy and France, respectively. A cross-sectional sample of 808 consumers (400 French and 408 Italian) completed a questionnaire. Structural equation modelling (SEM) indicated perceived behavioural control (PBC) and attitude to be significant predictors of intention to purchase PDO-labelled cheese in France and Italy. Subjective and moral norms affected intention in France. Intention significantly influenced the hard cheese purchase behaviour. The results confirm that the TPB model predicted the self-reported measure of behaviour more than the observed one, measured with a discrete choice experiment, in both countries. The TPB interrelationships varied between countries, suggesting that food systems operators and public authorities should carefully target their intervention to stimulate the demand of PDO-labelled products.

Previous studies have shown that credence attributes play an important and often undervalued role in consumers’ product evaluation and purchasing behavior. By contrast, the factors moderating these effects have received less attention by scholars. The current study uncovers the moderating effects of consumers’ psychographic characteristics (i.e.: subjective knowledge, wine involvement, and sustainability concern) on their preferences for wine with different credence attributes related to sustainability. A non-hypothetical economic experiment, consisting of three rounds (blindtaste, info, infotaste), was performed in Italy and Germany to assess consumers’ preferences in terms of willingness to pay for conventional wine and wine differentiated by wine sustainable certifications (i.e.: Organic, VIVA sustainable wine, Vignaioli Indipendenti, Carbon footprint). Wine sustainable certifications positively affect consumers’ preferences, and these effects are mainly moderated by individuals’ wine involvement and sustainability concern. This holds if respondents have information about the wine but do not taste it. After tasting the wine consumers’ preferences are moderated only by respondents’ sustainability concern.

This paper investigates consumers&rsquo, preferences for egg purchase in two European countries, Hungary and Italy. We utilize random parameter logit models to interpret the results of discrete choice experiments (DCE) for the elicitation of preference of the egg consumers. A sample of 403 in the Hungarian survey and 404 in the Italian survey were recruited in summer 2018. The DCE questionnaire includes the following product and process characteristics: organic labels, nutrition and health claims, and price. Our results show that for Hungarian and Italian consumers, the price is the most important attribute, followed by the nutrition and health claim and the organic production labelling. Three egg consumer segments can be identified via latent class models for each country. In both countries, we found similar consumer groups, the Price Sensitive and Quality Optimizing Opportunist Consumers and Health Conscious Buyers, respectively. Particularly, compared to the other segments the Health Conscious Buyers (46% in Hungary and 49% in Italy) exhibited stronger preference for and are willing to pay a higher price premium for eggs with organic label and nutrition claims. In Italy, we identified a third segment with consumers preferring simpler labelling approach, whilst in Hungary we found a consumer segment distrusting the EU organic logo.

In the present study, three main polysaccharide fractions, Xn-40, Xn-50, and Xn-80, were purified from fermentation broth cultures of Xylaria nigripes (Xns) by fractional precipitation with gradient concentrations of ethanol (40%, 50%, and 80%). In vitro antioxidant assay showed that Xn-40 was stronger than the others. The IC50 value of DPPH radical scavenging was 1,300 mg/L, and ABTS radical scavenging was 699.16 mg/L, respectively. Its functional group features were determined by Fourier transform-infrared spectroscopy (FT-IR). The results revealed the general characteristic absorption peaks of polysaccharides. We further investigated the antioxidant activity on human keratinocyte (HaCaT) cells. Our data demonstrated that polysaccharides of Xns had no cytotoxicity on HaCaT cells. Moreover, Xn-40 can significantly reduce AAPH-induced generates reactive oxygen species (ROS) generation in vitro. Comprehensive analysis results indicate that graded ethanol precipitation can effectively separate the polysaccharides that have antioxidant activity and Xns could be explored as a natural antioxidant.

Citral is a typical essential oil used in the food, cosmetic, and drug industries and has shown antimicrobial activity against microorganisms. Citral is unstable and hydrophobic under normal storage conditions, so it can easily lose its bactericide activity. Nanoemulsion technology is an excellent way to hydrophilize, microencapsulate, and protect this compound. In our studies, we used a mixed surfactant to form citral-in-water nanoemulsions, and attempted to optimize the formula for preparing nanoemulsions. Citral-in-water nanoemulsions formed at So 0.4 to 0.6 and ultrasonic power of 18 W for 120 seconds resulted in a droplet size of < 100 nm for nanoemulsions. The observed antimicrobial activities were significantly affected by the formulation of the nanoemulsions. The observed relationship between the formulation and activity can lead to the rational design of nanoemulsion-based delivery systems for essential oils, based on the desired function of antimicrobials in the food, cosmetics, and agrochemical industries.

This cross-country study investigates the relative role of organic labelling in consumers’ purchase decisions for apples and the extent to which behavioral constructs, derived from an extension of the Theory of Planned Behavior, influence consumers’ choices. We apply an Integrated Choice and Latent Variable Model, combining a discrete choice experiment with structural equation modelling. Empirical validation draws on data from an online survey conducted in three European countries (NGermany = 404; NNorway = 407; NUK = 401). In all countries, price is by far the most important attribute in consumers’ purchase decision of apples, followed by country-of-origin and production method. The results show considerable consistency across the investigated countries regarding the importance of behavioral constructs - attitudes, subjective norms, perceived behavioral control, trust, and personal moral norms - in explaining consumers’ intentions to buy, and purchase choices for, organic apples, confirming the derived theoretical framework. This study received funding from the European Union’s Horizon 2020 research and innovation programme as part of the STRENGTH2FOOD project under grant agreement No 678024.

Eco-labels are informational cues that transform credence into quasi-search attributes but their influence on consumer decision making is often disappointing. This paper identifies antecedents of trust in, and use of, eco-labels, validating a theoretical model with data for four publicly managed organic labels (the EU green leaf and three national labels from France, Germany and Serbia). Drawing on a large dataset of European consumers, the analysis reveals that the effect of institutional trust on use of an eco-label is mediated by trust in that eco-label. Trust in an eco-label positively affects use of that eco-label. Knowledge of third-party certification positively affects trust in, and use of, an eco-label. The results suggest that for eco-label managers wishing to increase consumer uptake of their labels, communicating third-party verification as is a critically important informational cue for enhancing consumer trust.

Background Children's dietary-related diseases and their associated costs have expanded dramatically in many countries, making children's food choice a policy issue of increasing relevance. As children spend a considerable amount of money on energy-dense, nutrient-poor (EDNP) products, a better understanding of the main drivers of children's independent food purchase decisions is crucial to move this behavior toward healthier options. Objective The objective of the study is to investigate the role of branding and price in motivating children to choose healthier snack options. Methods The study investigates snack choices of children ages 8 to 11, using a survey and a purchase experiment. The research took place in after-school programs of selected schools in the Boston area. Participants included 116 children. Products in the choice experiment differed on three factors: product type, brand, and price. Data were analyzed using aggregated and mixed logit models. Results Children's purchase decisions are primarily determined by product type (Importance Value (IV) 56.6%), while brand (IV 22.8%) and price (IV 20.6%) prove to be of less relevance. Only those children who state that they like the familiar brand reveal a preference for the branded product in their purchase decision. Price is a significant predictor of choice when controlling for whether or not children obtain an allowance. Conclusion It is not simple brand awareness but a child's liking of the brand that determines whether a brand is successful in motivating a child to choose a product. The extent of children's experience with money influences their price responsiveness. To the extent that children who receive an allowance are primarily the ones buying food snacks, higher prices for EDNP snacks could be successful in motivating children to choose a healthier option.

Governments around the world seek to create programs that will support sustainable agriculture and achieve food security, yet they are faced with uncertainty, system complexity and data scarcity when making such choices. We propose decision modeling as an innovative approach to help meet these challenges and offer a case study to show the effectiveness of the tool. We use decision analysis tools to model the possible nutrition-related outcomes of the Ugandan government's long term agricultural development plan termed ‘Vision 2040’. The analysis indicates potential shifts in household nutritional contributions through the comparison of the current small-scale diverse systems and the envisioned industrial agricultural systems that may replace them. A Monte Carlo simulation revealed that Vision 2040 plans outperform homegardens in terms of energy and some macronutrients, yet homegardens are likely to be better at producing key vitamins and micronutrients, such as Vitamin A. Value of information calculations applied to Monte Carlo outputs further revealed that gathering more data on the annual yields and nutrient contents of staples, pulses, vegetables, and fruits could improve certainty about the nutrition contribution of both scenarios. We conclude that the development of Uganda's agricultural sector should consider the role that agrobiodiversity in the current small-scale agricultural systems plays in national food and nutrition security. Any changes according to Vision 2040 should also include farmers' voices and current crop management systems as guides for a sustainable food supply in the region. This modeling approach may be a tool for governments to consider agricultural policy implications, especially given the data scarcity and agricultural variability in regions such as East Africa.

The presentation of credence attributes such as the product's origin or the production method has a significant influence on consumers' food purchase decisions. The dataset includes survey responses from a discrete choice experiment with 1309 food shoppers in Taiwan using the example of sweet pepper. The survey was carried out in 2014 in the three largest Taiwanese cities. It evaluates the impact of providing information on the equality of organic standards on consumers' preferences at the example of sweet pepper. Equality of organic standards implies that regardless of products’ country-of-origin (COO) organic certifications are based on the same production regulation and managerial processes. Respondents were randomly allocated to the information treatment and the control group. The dataset contains the product choices of participants in both groups, as well as their sociodemographic information., Data in Brief, 18, ISSN:2352-3409

Purpose Perineural invasion (PNI) is an adverse prognostic factor in patients with oral cavity squamous cell carcinoma (OCSCC). The American Joint Committee on Cancer Staging Manual, eighth edition, introduced a subdivision of PNI into two distinct forms, that is, extratumoral and intratumoral PNI (EPNI and IPNI, respectively). We designed the current study to assess whether EPNI and IPNI have different prognostic implications in terms of disease control and survival outcomes in patients with OCSCC. Materials and methods We retrospectively examined 229 consecutive patients with OCSCC and PNI who underwent radical surgery between July 2003 and November 2016. EPNI and IPNI were identified in 76 and 153 patients, respectively. The 5‐year locoregional control (LRC), distant metastasis, disease‐free survival (DFS), and overall survival (OS) rates served as the main outcome measures. Results Compared with patients showing IPNI, those with EPNI had a higher prevalence of worst pattern of invasion type‐5 (P, Extratumoral perineural invasion is significantly associated with poorer locoregional control, disease‐free survival and overall survival justifying its role as prognostic factor.

ObjectivesThe TBX5 gene, a member of the T-box family, is associated with congenital heart disease, electrocardiographic parameters, and development of atrial fibrillation in the general population. This study aimed to elucidate the role of TBX5 gene polymorphisms in metabolic and inflammatory profiles possibly linked to TBX5-related pathologies.Materials and MethodsA sample population of 597 individuals having routine health examinations was enrolled. Five tagging TBX5 single nucleotide polymorphisms (SNPs) were analyzed using polymerase chain reaction and restriction enzyme digestion or TaqMan SNP genotyping assays. Associations between genotypes/haplotypes and matrix metalloproteinase 9 (MMP9) levels were investigated using generalized linear model analysis. Interactions between each genotype/haplotype, MMP9 level, and obesity status were tested using two-way analysis of variance with Golden Helix SVS Win32 7.3.1 software.ResultsAfter adjusting for clinical covariates, TBX5 genotypes were found to be associated with MMP9 levels (p = 0.002 and p = 0.001 for rs4113925 and rs3825214, respectively) in a dominant inheritance model. Haplotype analysis using three tag SNPs (rs11067101, rs1247973, and rs3825214) revealed a significant association between TBX5 haplotype GCG and MMP9 levels (uncorrected p = 0.0093 and the corrected false discovery rate p = 0.0435). Multivariate analysis identified that SNP rs3825214, in addition to the MMP9 and E-selectin genotypes, was independently associated with MMP9 levels (p

Glycine N-methyltransferase (GNMT) is a tumor suppressor for HCC. It is down-regulated in HCC, but the mechanism is not fully understood. MicroRNA-224 (miR-224) acts as an onco-miR in HCC. This study is the first to investigate miR-224 targeting the coding region of GNMT transcript. The GNMT-MT plasmid containing a miR-224 binding site silent mutation of the GNMT coding sequence can escape the suppression of miR-224 in HEK293T cells. Expression of both exogenous and endogenous GNMT was suppressed by miR-224, while miR-224 inhibitor enhanced GNMT expression. miR-224 counteracts the effects of GNMT on the reduction of cell proliferation and tumor growth. The levels of miR-224 and GNMT mRNA showed a significant inverse relationship in tumor specimens from HCC patients. Utilizing CCl4-treated hepatoma cells and mice as a cell damage of inflammatory or liver injury model, we observed that the decreased expression levels of GNMT were accompanied with the elevated expression levels of miR-224 in hepatoma cells and mouse liver. Finally, hepatic AAV-mediated GNMT also reduced CCl4-induced miR-224 expression and liver fibrosis. These results indicated that AAV-mediated GNMT has potential liver protection activity. miR-224 can target the GNMT mRNA coding sequence and plays an important role in GNMT suppression during liver tumorigenesis.

Food Quality and Preference, 65, ISSN:0950-3293, ISSN:1873-6343

Bioplastics have been introduced as an alternative to petro-based plastics and to provide packaging materials with improved biodegradability and compostability. Over the past few years, several studies have been conducted on bioplastics and their application in global food systems. Although the potential environmental benefits have been discussed, little in fact is known about the specific requirements for the application of bioplastics as packaging for organic food. In this policy brief, we examine the applicability of bioplastics packaging to organic food products, based on the perspectives of interviewed experts in industry and academia. We conclude that international regulations and standards for organic food production should include specifications on the use of bioplastics. This is necessary because consumers expect bioplastics to be an environmentally friendly packaging material. Yet bioplastic packaging remains problematic for producers and consumers of organic food, especially if the raw material is originally sourced from genetically modified plants. There is a need for clarification of the type of raw material that is suitable for use as packaging for organic food. Our findings should enhance understanding of the public's expectations and perceptions of bioplastics packaging with regard to environmental impacts and optimized organic food packaging. See the press release for this article.