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Measurements of the mean and turbulence characteristics in nonuniform open-channel flows were carried out using a 3D acoustic Doppler velocimeter. Both accelerating and decelerating flows were investigated. Analyses based on the Reynolds equation and the continuity equation of 2D open-channel flow show that a flow will be in equilibrium if the pressure-gradient parameter [Beta] is a constant at different sections along the flow direction. The experimental data show that all the flows investigated are in equilibrium. The effect of the flow nonuniformity on the mean velocity and turbulence profiles was also examined. The study shows that (1) the log law is still valid for both accelerating and decelerating flows in the inner region. The Coles law can be used for the entire region, but the wake-strength parameter II depends on the [Beta]-value; and (2) the turbulence intensities and the Reynolds stress decrease in accelerating flow and increase in decelerating flow, when compared with those in uniform flow. Finally, using the Reynolds equation and the continuity equation of 2D open-channel flow, the theoretical expressions for the distribution of vertical velocity and the Reynolds stress have been developed. The measured vertical velocity and the Reynolds stress profile compare well with the derived expressions.