34 results on '"Chiara Rinoldi"'
Search Results
2. Electrospun Poly(vinyl alcohol)-Based Conductive Semi-interpenetrating Polymer Network Fibrous Hydrogel: A Toolbox for Optimal Cross-Linking
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Anna Zakrzewska, Seyed Shahrooz Zargarian, Chiara Rinoldi, Arkadiusz Gradys, Dariusz Jarząbek, Michele Zanoni, Chiara Gualandi, Massimiliano Lanzi, and Filippo Pierini
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Materials of engineering and construction. Mechanics of materials ,TA401-492 - Published
- 2023
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3. 159. Aerobic Exercise And Scaffolds With Hierarchical Porosity Synergistically Promote Functional Recovery Post Volumetric Muscle Loss
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Christina Zhu, BS, BA, Karina Sklyar, Yori Endo, MD, Mohamadmahdi Samandari, PhD, Mehran Karvar, PhD, Azadeh Mostafavi, PhD, Jacob Quint, MS, Chiara Rinoldi, PhD, Wojciech Swieszkowski, PhD, Joshua Mauney, PhD, Shailesh Agarwal, MD, Ali Tamayol, PhD, and Indranil Sinha, MD
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Surgery ,RD1-811 - Published
- 2023
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4. Near‐infrared light activated core‐shell electrospun nanofibers decorated with photoactive plasmonic nanoparticles for on‐demand smart drug delivery applications
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Mohammad Ali Haghighat Bayan, Yasmin Juliane Dias, Chiara Rinoldi, Paweł Nakielski, Daniel Rybak, Yen B. Truong, Alexander L. Yarin, and Filippo Pierini
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Polymers and Plastics ,Materials Chemistry ,Physical and Theoretical Chemistry - Published
- 2023
5. Evolution of nanostructured skin patches towards multifunctional wearable platforms for biomedical applications
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Daniel Rybak, Yu-Chia Su, Yang Li, Bin Ding, Xiaoshuang Lv, Zhaoling Li, Yi-Cheun Yeh, Pawel Nakielski, Chiara Rinoldi, Filippo Pierini, and Jagan Mohan Dodda
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General Materials Science - Abstract
Skin patches (SPs) have rapidly advanced to rehabilitation, health monitoring, self-powered and integrated systems. Accordingly, design of nanomaterials, flexible substrates, hydrogels and nanofibers can facilitate the therapeutic application of SPs.
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- 2023
6. In vitro functional models for human liver diseases and drug screening: beyond animal testing
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Alessia Paradiso, Marina Volpi, Chiara Rinoldi, Nehar Celikkin, Nicola Contessi Negrini, Muge Bilgen, Giorgio Dallera, Filippo Pierini, Marco Costantini, Wojciech Święszkowski, and Silvia Farè
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Biomedical Engineering ,General Materials Science - Abstract
In this review, in vitro functional models for human liver diseases and drug testing as an alternative to animal testing are described and discussed, highlighting pros and cons of the current state of the art reported in the scientific literature.
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- 2023
7. In Vivo Chronic Brain Cortex Signal Recording Based on a Soft Conductive Hydrogel Biointerface
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Chiara Rinoldi, Yasamin Ziai, Seyed Shahrooz Zargarian, Paweł Nakielski, Krzysztof Zembrzycki, Mohammad Ali Haghighat Bayan, Anna Beata Zakrzewska, Roberto Fiorelli, Massimiliano Lanzi, Agnieszka Kostrzewska-Księżyk, Rafał Czajkowski, Ewa Kublik, Leszek Kaczmarek, and Filippo Pierini
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General Materials Science - Published
- 2022
8. Conducting polymer‐based nanostructured materials for brain–machine interfaces
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Yasamin Ziai, Seyed Shahrooz Zargarian, Chiara Rinoldi, Paweł Nakielski, Antonella Sola, Massimiliano Lanzi, Yen Bach Truong, and Filippo Pierini
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Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering - Published
- 2023
9. Nanoengineered myogenic scaffolds for skeletal muscle tissue engineering
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Jacob P. Quint, Mohamadmahdi Samandari, Laleh Abbasi, Evelyn Mollocana, Chiara Rinoldi, Azadeh Mostafavi, and Ali Tamayol
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Tissue Engineering ,Gelatin ,General Materials Science ,Hydrogels ,Muscle Development ,Muscle, Skeletal ,Article - Abstract
Extreme loss of skeletal muscle overwhelms the natural regenerative capability of the body, results in permanent disability and substantial economic burden. Current surgical techniques result in poor healing, secondary injury to the autograft donor site, and incomplete recuperation of muscle function. Most current tissue engineering and regenerative strategies fail to create an adequate mechanical and biological environment that enables cell infiltration, proliferation, and myogenic differentiation. In this study, we present a nanoengineered skeletal muscle scaffold based on functionalized gelatin methacrylate (GelMA) hydrogel, optimized for muscle progenitors’ proliferation and differentiation. The scaffold was capable of controlling the release of insulin-like growth factor 1 (IGF-1), an important myogenic growth factor, by utilizing the electrostatic interactions with Laponite® nanoclays (NCs). Physiologically relevant levels of IGF-1 were maintained during a controlled release over two weeks. The NC was able to retain 50% of the released IGF-1 within the hydrogel niche, significantly improving cellular proliferation and differentiation compared to control hydrogels. IGF-1 supplemented medium controls required 44% more IGF-1 than the comparable NC hydrogel composites. The nanofunctionalized scaffold is a viable option for the treatment of extreme muscle injuries and offers scalable benefits for translational interventions and the growing field of clean meat production.
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- 2023
10. Aerobic exercise and scaffolds with hierarchical porosity synergistically promote functional recovery post volumetric muscle loss
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Yori Endo, Mohamadmahdi Samandari, Mehran Karvar, Azadeh Mostafavi, Jacob Quint, Chiara Rinoldi, Iman K. Yazdi, Wojciech Swieszkowski, Joshua Mauney, Shailesh Agarwal, Ali Tamayol, and Indranil Sinha
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Colloidal scaffolds ,Exercise therapy ,Biomedical Engineering ,Biophysics ,Bioengineering ,Regenerative Medicine ,In situ printing ,Biomaterials ,Mice ,Muscular Diseases ,Animals ,Regeneration ,Volumetric muscle loss ,Insulin-Like Growth Factor I ,Physical Therapy Modalities ,Tissue Engineering ,Tissue Scaffolds ,5.2 Cellular and gene therapies ,Skeletal ,Stem Cell Research ,Fibrosis ,Mechanics of Materials ,Musculoskeletal ,IGF-1 ,Ceramics and Composites ,Muscle ,Stem Cell Research - Nonembryonic - Non-Human ,Development of treatments and therapeutic interventions ,Porosity - Abstract
Volumetric muscle loss (VML), which refers to a composite skeletal muscle defect, most commonly heals by scarring and minimal muscle regeneration but substantial fibrosis. Current surgical interventions and physical therapy techniques are limited in restoring muscle function following VML. Novel tissue engineering strategies may offer an option to promote functional muscle recovery. The present study evaluates a colloidal scaffold with hierarchical porosity and controlled mechanical properties for the treatment of VML. In addition, as VML results in an acute decrease in insulin-like growth factor 1 (IGF-1), a myogenic factor, the scaffold was designed to slowly release IGF-1 following implantation. The foam-like scaffold is directly crosslinked onto remnant muscle without the need for suturing. In situ 3D printing of IGF-1-releasing porous muscle scaffold onto VML injuries resulted in robust tissue ingrowth, improved muscle repair, and increased muscle strength in a murine VML model. Histological analysis confirmed regeneration of new muscle in the engineered scaffolds. In addition, the scaffolds significantly reduced fibrosis and increased the expression of neuromuscular junctions in the newly regenerated tissue. Exercise training, when combined with the engineered scaffolds, augmented the treatment outcome in a synergistic fashion. These data suggest highly porous scaffolds and exercise therapy, in combination, may be a treatment option following VML.
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- 2023
11. Chameleon-inspired multifunctional plasmonic nanoplatforms for biosensing applications
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Yasamin Ziai, Francesca Petronella, Chiara Rinoldi, Paweł Nakielski, Anna Zakrzewska, Tomasz A. Kowalewski, Weronika Augustyniak, Xiaoran Li, Antonella Calogero, Izabela Sabała, Bin Ding, Luciano De Sio, and Filippo Pierini
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biology ,Modeling and Simulation ,General Materials Science ,biosensing ,therma-therapy ,Condensed Matter Physics ,plasmonics - Abstract
One of the most fascinating areas in the field of smart biopolymers is biomolecule sensing. Accordingly, multifunctional biomimetic, biocompatible, and stimuli-responsive materials based on hydrogels have attracted much interest. Within this framework, the design of nanostructured materials that do not require any external energy source is beneficial for developing a platform for sensing glucose in body fluids. In this article, we report the realization and application of an innovative platform consisting of two outer layers of a nanocomposite plasmonic hydrogel plus one inner layer of electrospun mat fabricated by electrospinning, where the outer layers exploit photoinitiated free radical polymerization, obtaining a compact and stable device. Inspired by the exceptional features of chameleon skin, plasmonic silver nanocubes are embedded into a poly(N-isopropylacrylamide)-based hydrogel network to obtain enhanced thermoresponsive and antibacterial properties. The introduction of an electrospun mat creates a compatible environment for the homogeneous hydrogel coating while imparting excellent mechanical and structural properties to the final system. Chemical, morphological, and optical characterizations were performed to investigate the structure of the layers and the multifunctional platform. The synergetic effect of the nanostructured system’s photothermal responsivity and antibacterial properties was evaluated. The sensing features associated with the optical properties of silver nanocubes revealed that the proposed multifunctional system is a promising candidate for glucose-sensing applications.
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- 2022
12. Smart plasmonic hydrogels based on gold and silver nanoparticles for biosensing application
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Yasamin Ziai, Chiara Rinoldi, Paweł Nakielski, Luciano De Sio, and Filippo Pierini
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Biomaterials ,plasmonic hydrogel ,smart materials ,Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,biosensing - Published
- 2022
13. Cholesteryl Ester Liquid Crystal Nanofibers for Tissue Engineering Applications
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Azadeh Mostafavi, Sina Sharifi, Amir Nasajpour, Steven J. Jonas, Matthew Ye, Ali Tamayol, Matthew Ji, Chiara Rinoldi, Adrian Chlanda, Paul S. Weiss, Wojciech Swieszkowski, and Ali Khademhosseini
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chemistry.chemical_compound ,Materials science ,Tissue engineering ,chemistry ,Chemical engineering ,Liquid crystal ,General Chemical Engineering ,Nanofiber ,Biomedical Engineering ,Cholesteryl ester ,General Materials Science - Abstract
Liquid-crystal-based biomaterials provide promising platforms for the development of dynamic and responsive interfaces for tissue engineering. Cholesteryl ester liquid crystals (CLCs) are particula...
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- 2020
14. Nanotechnology-Assisted RNA Delivery: From Nucleic Acid Therapeutics to COVID-19 Vaccines
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Xiaoran Li, Filippo Pierini, Seyed Shahrooz Zargarian, Marco Costantini, Chiara Gualandi, Bin Ding, Anna Liguori, Chiara Rinoldi, Paweł Nakielski, Dario Presutti, Qiusheng Wang, Luciano De Sio, and Francesca Petronella
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Engineering ,2019-20 coronavirus outbreak ,COVID-19 Vaccines ,Coronavirus disease 2019 (COVID-19) ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Reviews ,Nanotechnology ,Review ,Drug Delivery Systems ,COVID‐19 ,Nucleic Acids ,ribonucleic acids ,Humans ,General Materials Science ,Personalized therapy ,personalized therapy ,business.industry ,SARS-CoV-2 ,RNA ,COVID-19 ,General Chemistry ,nanostructured biomaterials ,Nanostructures ,Pharmaceutical Preparations ,drug delivery ,Nucleic acid ,Personalized medicine ,business - Abstract
In recent years, the main quest of science has been the pioneering of the groundbreaking biomedical strategies needed for achieving a personalized medicine. Ribonucleic acids (RNAs) are outstanding bioactive macromolecules identified as pivotal actors in regulating a wide range of biochemical pathways. The ability to intimately control the cell fate and tissue activities makes RNA‐based drugs the most fascinating family of bioactive agents. However, achieving a widespread application of RNA therapeutics in humans is still a challenging feat, due to both the instability of naked RNA and the presence of biological barriers aimed at hindering the entrance of RNA into cells. Recently, material scientists’ enormous efforts have led to the development of various classes of nanostructured carriers customized to overcome these limitations. This work systematically reviews the current advances in developing the next generation of drugs based on nanotechnology‐assisted RNA delivery. The features of the most used RNA molecules are presented, together with the development strategies and properties of nanostructured vehicles. Also provided is an in‐depth overview of various therapeutic applications of the presented systems, including coronavirus disease vaccines and the newest trends in the field. Lastly, emerging challenges and future perspectives for nanotechnology‐mediated RNA therapies are discussed., Nanotechnology‐mediated RNA delivery enables regulating a broad range of cellular processes providing effective strategies for personalized medicine. This review provides a comprehensive overview of the recent evidence in the field, highlighting key RNA molecules and nanostructured carriers. Their innovative applications for vaccine development, wound healing, cancer, and neural system treatments are summarized. Finally, new trends and future applications are discussed.
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- 2021
15. Customizable Composite Fibers for Engineering Skeletal Muscle Models
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Ludovic Serex, Farhang Tarlan, Su Ryon Shin, Fatemeh Sharifi, Raquel Costa-Almeida, Huseyin Avci, Ali Tamayol, Chiara Rinoldi, Iman K. Yazdi, Ali Khademhosseini, Emal Lesha, Negar Faramarzi, Manuela E. Gomes, Afsoon Fallahi, Mohsen Akbari, and Universidade do Minho
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Materials science ,education ,0206 medical engineering ,Composite number ,Biomedical Engineering ,02 engineering and technology ,reinforced fibers ,Biomaterials ,Tissue engineering ,Biotecnologia Médica [Ciências Médicas] ,medicine ,biotextiles ,Composite Fibers ,Muscle, Skeletal ,Topology (chemistry) ,Cell Proliferation ,Science & Technology ,Tissue Engineering ,Tissue Scaffolds ,fungi ,food and beverages ,Skeletal muscle ,Myogenesis ,Hydrogels ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,humanities ,3. Good health ,medicine.anatomical_structure ,tissue engineering ,Ciências Médicas::Biotecnologia Médica ,interpenetrating network hydrogels ,organ weaving ,0210 nano-technology ,skeletal muscles ,Biomedical engineering ,TE - Abstract
Published online: 14 November 2019, Engineering tissue-like scaffolds that can mimic the microstructure, architecture, topology, and mechanical properties of native tissues while offering an excellent environment for cellular growth has remained an unmet need. To address these challenges, multicompartment composite fibers are fabricated. These fibers can be assembled through textile processes to tailor tissue-level mechanical and electrical properties independent of cellular level components. Textile technologies also allow control of the distribution of different cell types and the microstructure of fabricated constructs and the direction of cellular growth within the 3D microenvironment. Here, we engineered composite fibers from biocompatible cores and biologically relevant hydrogel sheaths. The fibers are mechanically robust to being assembled using textile processes and could support adhesion, proliferation, and maturation of cell populations important for the engineering of skeletal muscles. We also demonstrated that the changes in the coating of the multicompartment fibers could potentially enhance myogenesis in vitro., S.R.S. was partially supported by the National Institutes of Health (R21EB026824) and the Brigham Research Institute Stepping Strong Innovator Award. C.R. would like to thank the funding from the National Centre for Research and Develop ment (STRATEGMED1/233224/10/NCBR/2014, project START). This work was partially supported by the National Institutes of Health (AR066193, AR066193, EB022403, AR057837, HL137193, GM126831, AR073822). M.A. would like to thank the Natural Sciences and Engineering Research Council of Canada (NSERC). H.A. thanks the Scientific and Technological Research Council of Turkey (TUBITAK). Additionally, I.K.Y. acknowledges financial support from the NIH through the Organ Design and Engineering Training program (T32 EB16652); R.C.-A. thanks the Portuguese funds through FCT−Fundação para a Ciência e a Tecnologia in the framework of FCT-POPH-FSE, the Ph.D. grant SFRH/BD/96593/2013.
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- 2021
16. Three-Dimensional Printable Conductive Semi-Interpenetrating Polymer Network Hydrogel for Neural Tissue Applications
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Wojciech Maksymowicz, Roberto Fiorelli, Nader Sanai, Chiara Rinoldi, Renata Bilewicz, Massimiliano Lanzi, Katarzyna Jezierska-Woźniak, Paweł Nakielski, Tomasz Kowalewski, Dario Pisignano, Krzysztof Zembrzycki, Andrea Camposeo, Olga Urbanek, Valentina Grippo, Filippo Pierini, Rinoldi C., Lanzi M., Fiorelli R., Nakielski P., Zembrzycki K., Kowalewski T., Urbanek O., Grippo V., Jezierska-Wozniak K., Maksymowicz W., Camposeo A., Bilewicz R., Pisignano D., Sanai N., and Pierini F.
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Materials science ,Polymers and Plastics ,Polymers ,Electric Conductivity ,Tissue Engineering ,Hydrogels ,Nerve Tissue ,Bioengineering ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Article ,Neural tissue engineering ,Biomaterials ,chemistry.chemical_compound ,Tissue engineering ,Materials Chemistry ,Interpenetrating polymer network ,electrical propertie ,chemistry.chemical_classification ,Conductive polymer ,Laser printing ,Polymer ,3D printing ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,laser ,chemistry ,Self-healing hydrogels ,Polythiophene ,hydrogel ,0210 nano-technology ,WATER-SOLUBLE POLYTHIOPHENES ,MECHANICAL-PROPERTIES ,PHOTOVOLTAIC PERFORMANCE ,HYBRID HYDROGELS ,GRAPHENE OXIDE ,BIOCOMPATIBILITY ,BIOMATERIALS ,COMPOSITES ,PNIPAM - Abstract
Intrinsically conducting polymers (ICPs) are widely used to fabricate biomaterials; their application in neural tissue engineering, however, is severely limited because of their hydrophobicity and insufficient mechanical properties. For these reasons, soft conductive polymer hydrogels (CPHs) are recently developed, resulting in a water-based system with tissue-like mechanical, biological, and electrical properties. The strategy of incorporating ICPs as a conductive component into CPHs is recently explored by synthesizing the hydrogel around ICP chains, thus forming a semi-interpenetrating polymer network (semi-IPN). In this work, a novel conductive semi-IPN hydrogel is designed and synthesized. The hybrid hydrogel is based on a poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide) hydrogel where polythiophene is introduced as an ICP to provide the system with good electrical properties. The fabrication of the hybrid hydrogel in an aqueous medium is made possible by modifying and synthesizing the monomers of polythiophene to ensure water solubility. The morphological, chemical, thermal, electrical, electrochemical, and mechanical properties of semi-IPNs were fully investigated. Additionally, the biological response of neural progenitor cells and mesenchymal stem cells in contact with the conductive semi-IPN was evaluated in terms of neural differentiation and proliferation. Lastly, the potential of the hydrogel solution as a 3D printing ink was evaluated through the 3D laser printing method. The presented results revealed that the proposed 3D printable conductive semi-IPN system is a good candidate as a scaffold for neural tissue applications.
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- 2021
17. Thermoactive Smart Electrospun Nanofibers
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Anna Liguori, Stefano Pandini, Chiara Rinoldi, Nelsi Zaccheroni, Filippo Pierini, Maria Letizia Focarete, Chiara Gualandi, Liguori A., Pandini S., Rinoldi C., Zaccheroni N., Pierini F., Focarete M.L., and Gualandi C.
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thermoelectric material ,Polymers and Plastics ,thermoelectric materials ,Organic Chemistry ,Temperature ,Nanofibers ,shape memory polymers ,thermoresponsive material ,thermoresponsive materials ,shape memory polymer ,phase change materials ,electrospinning ,pyroelectric materials ,thermo-optically responsive materials ,thermo-optically responsive material ,Materials Chemistry ,phase change material ,pyroelectric material - Abstract
The recent burst of research on smart materials is a clear evidence of the growing interest of the scientific community, industry, and society in the field. The exploitation of the great potential of stimuli-responsive materials for sensing, actuation, logic, and control applications is favored and supported by new manufacturing technologies, such as electrospinning, that allows to endow smart materials with micro- and nanostructuration, thus opening up additional and unprecedented prospects. In this wide and lively scenario, this article systematically reviews the current advances in the development of thermoactive electrospun fibers and textiles, sorting them, according to their response to the thermal stimulus. Hence, several platforms including thermoresponsive systems, shape memory polymers, thermo-optically responsive systems, phase change materials, thermoelectric materials, and pyroelectric materials, are described and critically discussed. The difference in active species and outputs of the aforementioned categories is highlighted, evidencing the transversal nature of temperature stimulus. Moreover, the potential of novel thermoactive materials are pointed out, revealing how their development could take to utmost interesting achievements.
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- 2022
18. Multifunctional platform based on electrospun nanofibers and plasmonic hydrogel. A smart nanostructured pillow for near-infrared light-driven biomedical applications
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Tomasz Kowalewski, Filippo Pierini, Sylwia Pawłowska, Luciano De Sio, Massimiliano Lanzi, Paweł Nakielski, Elisabetta Salatelli, Michał Pruchniewski, Krzysztof Zembrzycki, Chiara Rinoldi, Alexander L. Yarin, Antonella Calogero, Olga Urbanek, Yasamin Ziai, Nakielski, Paweł, Pawłowska, Sylwia, Rinoldi, Chiara, Ziai, Yasamin, De Sio, Luciano, Urbanek, Olga, Zembrzycki, Krzysztof, Pruchniewski, Michał, Lanzi, Massimiliano, Salatelli, Elisabetta, Calogero, Antonella, Kowalewski, Tomasz A, Yarin, Alexander L, and Pierini, Filippo
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multifunctional platform ,plasmonic hydrogel ,Swine ,Water flow ,Nanofibers ,Biocompatible Materials ,02 engineering and technology ,01 natural sciences ,Nanomaterials ,Mice ,Cell Movement ,General Materials Science ,Skin ,NIR-light responsive nanomaterial ,Drug Carriers ,bioinspired materials ,Hydrogels ,021001 nanoscience & nanotechnology ,Controlled release ,electrospun nanofibers ,NIR-light responsive nanomaterials ,Drug delivery ,0210 nano-technology ,Porosity ,electrospun nanofiber ,Materials science ,Biocompatibility ,Cell Survival ,Infrared Rays ,Polyesters ,Nanotechnology ,photothermal-based polytherapy ,010402 general chemistry ,Bioinspired material ,Cell Line ,Animals ,Humans ,Plasmon ,on-demand drug delivery ,Rhodamines ,technology, industry, and agriculture ,Photothermal therapy ,Nanostructures ,0104 chemical sciences ,multifunctional platforms ,Drug Liberation ,Microscopy, Fluorescence ,Nanofiber ,Gold - Abstract
Multifunctional nanomaterials with the ability to respond to near-infrared (NIR) light stimulation are vital for the development of highly efficient biomedical nanoplatforms with a polytherapeutic approach. Inspired by the mesoglea structure of jellyfish bells, a biomimetic multifunctional nanostructured pillow with fast photothermal responsiveness for NIR light-controlled on-demand drug delivery is developed. We fabricate a nanoplatform with several hierarchical levels designed to generate a series of controlled, rapid, and reversible cascade-like structural changes upon NIR light irradiation. The mechanical contraction of the nanostructured platform, resulting from the increase of temperature to 42 °C due to plasmonic hydrogel-light interaction, causes a rapid expulsion of water from the inner structure, passing through an electrospun membrane anchored onto the hydrogel core. The mutual effects of the rise in temperature and water flow stimulate the release of molecules from the nanofibers. To expand the potential applications of the biomimetic platform, the photothermal responsiveness to reach the typical temperature level for performing photothermal therapy (PTT) is designed. The on-demand drug model penetration into pig tissue demonstrates the efficiency of the nanostructured platform in the rapid and controlled release of molecules, while the high biocompatibility confirms the pillow potential for biomedical applications based on the NIR light-driven multitherapy strategy.
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- 2020
19. Drug delivery systems and materials for wound healing applications
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Fatemeh Sharifi, Ali Khademhosseini, Hossein Derakhshandeh, Maik Schot, Kan Yue, Giorgio Giatsidis, Sara Saheb Kashaf, Ali Tamayol, Pooria Mostafalu, Kristo Nuutila, Saghi Saghazadeh, Elmira Jalilian, Adnan Memic, Wojciech Swieszkowski, and Chiara Rinoldi
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medicine.medical_specialty ,Microtechnologies ,UT-Hybrid-D ,Pharmaceutical Science ,Biocompatible Materials ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Article ,Wound care ,Drug Delivery Systems ,Transdermal delivery ,Humans ,Medicine ,Intensive care medicine ,Skin ,Wound Healing ,integumentary system ,business.industry ,Nanotechnologies ,Limb amputation ,021001 nanoscience & nanotechnology ,Elevated ph ,0104 chemical sciences ,Impaired mobility ,Drug delivery ,Wound closure ,0210 nano-technology ,business ,Wound healing ,Healthcare system - Abstract
Chronic, non-healing wounds place a significant burden on patients and healthcare systems, resulting in impaired mobility, limb amputation, or even death. Chronic wounds result from a disruption in the highly orchestrated cascade of events involved in wound closure. Significant advances in our understanding of the pathophysiology of chronic wounds have resulted in the development of drugs designed to target different aspects of the impaired processes. However, the hostility of the wound environment rich in degradative enzymes and its elevated pH, combined with differences in the time scales of different physiological processes involved in tissue regeneration require the use of effective drug delivery systems. In this review, we will first discuss the pathophysiology of chronic wounds and then the materials used for engineering drug delivery systems. Different passive and active drug delivery systems used in wound care will be reviewed. In addition, the architecture of the delivery platform and its ability to modulate drug delivery are discussed. Emerging technologies and the opportunities for engineering more effective wound care devices are also highlighted.
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- 2018
20. Nanobead-on-string composites for tendon tissue engineering
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Ali Khademhosseini, Ali Tamayol, Chiara Rinoldi, Wojciech Swieszkowski, Adrian Chlanda, Ewa Kijeńska, Nabyl Khenoussi, and Emilia Choińska
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Scaffold ,Materials science ,Nanocomposite ,Regeneration (biology) ,Composite number ,technology, industry, and agriculture ,Biomedical Engineering ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,General Chemistry ,General Medicine ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Biodegradable polymer ,Electrospinning ,0104 chemical sciences ,Tissue engineering ,General Materials Science ,0210 nano-technology - Abstract
Tissue engineering holds great potential in the production of functional substitutes to restore, maintain or improve the functionality in defective or lost tissues. So far, a great variety of techniques and approaches for fabrication of scaffolds have been developed and evaluated, allowing researchers to tailor precisely the morphological, chemical and mechanical features of the final constructs. Electrospinning of biocompatible and biodegradable polymers is a popular method for producing homogeneous nanofibrous structures, which might reproduce the nanosized organization of the tendons. Moreover, composite scaffolds obtained by incorporating nanoparticles within electrospun fibers have been lately explored in order to enhance the properties and the functionalities of the pristine polymeric constructs. The present study is focused on the design and fabrication of biocompatible electrospun nanocomposite fibrous scaffolds for tendon regeneration. A mixture of poly(amide 6) and poly(caprolactone) is electrospun to generate constructs with mechanical properties comparable to that of native tendons. To improve the biological activity of the constructs and modify their topography, wettability, stiffness and degradation rate, we incorporated silica particles into the electrospun substrates. The use of nanosize silica particles enables us to form bead-on-fiber topography, allowing the better exposure of ceramic particles to better profit their beneficial characteristics. In vitro biocompatibility studies using L929 fibroblasts demonstrated that the presence of 20 wt% of silica nanoparticles in the engineered scaffolds enhanced cell spreading and proliferation as well as extracellular matrix deposition. The results reveal that the electrospun nanocomposite scaffold represents an interesting candidate for tendon tissue engineering.
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- 2018
21. Laser‐Assisted Fabrication of Injectable Nanofibrous Cell Carriers
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Małgorzata Gazińska, Emilia Sinderewicz, Michał Pruchniewski, Filippo Pierini, Joanna Staszkiewicz-Chodor, Paweł Nakielski, Piotr Denis, Monika Barczewska, Barbara Strojny, Wojciech Maksymowicz, Olga Urbanek, Chiara Rinoldi, Wioleta Czelejewska, Sylwia Pawłowska, Katarzyna Jezierska-Woźniak, Daniel Rybak, Marta Grodzik, and Yasamin Ziai
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Fabrication ,Materials science ,Biocompatibility ,Cell ,Nanofibers ,Biocompatible Materials ,law.invention ,Biomaterials ,Chitosan ,chemistry.chemical_compound ,law ,medicine ,General Materials Science ,3D bioprinting ,Tissue Engineering ,Tissue Scaffolds ,Lasers ,General Chemistry ,Laser assisted ,Cell delivery ,digestive system diseases ,Extracellular Matrix ,medicine.anatomical_structure ,chemistry ,Ex vivo ,Biotechnology ,Biomedical engineering - Abstract
The use of injectable biomaterials for cell delivery is a rapidly expanding field which may revolutionize the medical treatments by making them less invasive. However, creating desirable cell carriers poses significant challenges to the clinical implementation of cell-based therapeutics. At the same time, no method has been developed to produce injectable microscaffolds (MSs) from electrospun materials. Here the fabrication of injectable electrospun nanofibers is reported on, which retain their fibrous structure to mimic the extracellular matrix. The laser-assisted micro-scaffold fabrication has produced tens of thousands of MSs in a short time. An efficient attachment of cells to the surface and their proliferation is observed, creating cell-populated MSs. The cytocompatibility assays proved their biocompatibility, safety, and potential as cell carriers. Ex vivo results with the use of bone and cartilage tissues proved that NaOH hydrolyzed and chitosan functionalized MSs are compatible with living tissues and readily populated with cells. Injectability studies of MSs showed a high injectability rate, while at the same time, the force needed to eject the load is no higher than 25 N. In the future, the produced MSs may be studied more in-depth as cell carriers in minimally invasive cell therapies and 3D bioprinting applications.
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- 2021
22. Mechanical and Biochemical Stimulation of 3D Multilayered Scaffolds for Tendon Tissue Engineering
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Danilo Demarchi, Grissel Trujillo-de Santiago, Afsoon Fallahi, Jessica Campos Paras, Iman K. Yazdi, Ali Khademhosseini, Nasim Annabi, Ewa Kijeńska-Gawrońska, Chiara Rinoldi, Ali Tamayol, Abuduwaili Tuoheti, and Wojciech Swieszkowski
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Chemistry ,Cellular differentiation ,Mesenchymal stem cell ,Biomedical Engineering ,Stimulation ,Tendon tissue ,Bone morphogenetic protein ,Tendon ,Biomaterials ,medicine.anatomical_structure ,medicine ,Bioreactor ,nanofibrous materials ,composite scaffolds ,mechanical stimulation ,stem cell differentiation ,tendon tissue engineering ,Viability assay ,Biomedical engineering - Abstract
Tendon injuries are frequent and occur in the elderly, young, and athletic populations. The inadequate number of donors combined with many challenges associated with autografts, allografts, xenografts, and prosthetic devices have added to the value of engineering biological substitutes, which can be implanted to repair the damaged tendons. Electrospun scaffolds have the potential to mimic the native tissue structure along with desired mechanical properties and, thus, have attracted noticeable attention. In order to improve the biological responses of these fibrous structures, we designed and fabricated 3D multilayered composite scaffolds, where an electrospun nanofibrous substrate was coated with a thin layer of cell-laden hydrogel. The whole construct composition was optimized to achieve adequate mechanical and physical properties as well as cell viability and proliferation. Mesenchymal stem cells (MSCs) were differentiated by the addition of bone morphogenetic protein 12 (BMP-12). To mimic the natural function of tendons, the cell-laden scaffolds were mechanically stimulated using a custom-built bioreactor. The synergistic effect of mechanical and biochemical stimulation was observed in terms of enhanced cell viability, proliferation, alignment, and tenogenic differentiation. The results suggested that the proposed constructs can be used for engineering functional tendons.
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- 2019
23. Engineering biological gradients
- Author
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Daniela Peneda Pacheco, Laura Zorzetto, Lorenzo Sardelli, Wojciech Święszkowski, Paola Petrini, and Chiara Rinoldi
- Subjects
Rapid prototyping ,Computer science ,Polymers ,lcsh:Biotechnology ,Biomedical Engineering ,Biophysics ,microfluidic ,Bioengineering ,02 engineering and technology ,Regenerative Medicine ,Regenerative medicine ,bone ,Biomaterials ,03 medical and health sciences ,Graded scaffolds, rapid prototyping, bioinspired, microfluidic, gradient characterization, cartilage, bone ,lcsh:TP248.13-248.65 ,Elastic Modulus ,Lab-On-A-Chip Devices ,gradient characterization ,Humans ,bioinspired ,cartilage ,030304 developmental biology ,rapid prototyping ,0303 health sciences ,Tissue Engineering ,Tissue Scaffolds ,Bioprinting ,General Medicine ,021001 nanoscience & nanotechnology ,Characterization (materials science) ,Freeze Drying ,Graded scaffolds ,Biochemical engineering ,0210 nano-technology - Abstract
Biological gradients profoundly influence many cellular activities, such as adhesion, migration, and differentiation, which are the key to biological processes, such as inflammation, remodeling, and tissue regeneration. Thus, engineered structures containing bioinspired gradients can not only support a better understanding of these phenomena, but also guide and improve the current limits of regenerative medicine. In this review, we outline the challenges behind the engineering of devices containing chemical-physical and biomolecular gradients, classifying them according to gradient-making methods and the finalities of the systems. Different manufacturing processes can generate gradients in either in-vitro systems or scaffolds, which are suitable tools for the study of cellular behavior and for regenerative medicine; within these, rapid prototyping techniques may have a huge impact on the controlled production of gradients. The parallel need to develop characterization techniques is addressed, underlining advantages and weaknesses in the analysis of both chemical and physical gradients.
- Published
- 2019
24. 3D Tissue Modelling of Skeletal Muscle Tissue
- Author
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Cesare Gargioli, Chiara Rinoldi, Nehar Celikkin, Wojciech Święszkowski, Stefano Testa, Marco Costantini, Cristina Colosi, Joanna Idaszek, and Andrea Barbetta
- Subjects
Muscle tissue ,skeletal muscle tissue ,business.industry ,Mechanism (biology) ,Regeneration (biology) ,Cellular differentiation ,bioprinting, skeletal muscle tissue, biomaterials ,Skeletal muscle ,Cancer resection ,medicine.anatomical_structure ,Skeletal Muscle Tissue ,Medicine ,business ,bioprinting ,Neuroscience ,Process (anatomy) ,biomaterials - Abstract
Skeletal muscle tissue exhibits an endogenous ability to regenerate. However, the self-repair mechanism is restricted only to minor damage. The increasing number of extensive injuries of skeletal muscles due to various accidents, a more active life-style or cancer resection, combined with the shortcomings of conventional treatment procedures, creates a demand for new, more advanced solutions. Muscle tissue engineering (TE) appears a promising strategy for the fabrication of tissue substitutes from biomaterials, cells and bioactive factors, alone or combined. In this chapter, we present current state of the art of regeneration and engineering of skeletal muscle tissue. The chapter begins with a brief introduction to the structure and functions of skeletal muscle tissue, followed by discussion of cells with potential for repair of muscle injuries and dysfunctions. Next, we provide an overview of natural and synthetic biomaterials used in skeletal muscle TE, as well as description of techniques used to process the biomaterials into scaffolds. We also highlight the importance of mechanical and electrical stimulation during in vitro culture and their effect on cell differentiation and maturation. Last but not least, the latest results of in vivo studies are reported. The chapter is concluded with a short summary and outlook on future developments.
- Published
- 2019
25. Fibrous Systems as Potential Solutions for Tendon and Ligament Repair, Healing, and Regeneration
- Author
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Wojciech Swieszkowski, Chiara Rinoldi, Ali Khademhosseini, Ali Tamayol, and Ewa Kijeńska-Gawrońska
- Subjects
Scaffold ,Population ,Biomedical Engineering ,Pharmaceutical Science ,02 engineering and technology ,Regenerative Medicine ,010402 general chemistry ,01 natural sciences ,Regenerative medicine ,Article ,Tendons ,Biomaterials ,Tissue engineering ,Ligament repair ,Humans ,Medicine ,education ,education.field_of_study ,Ligaments ,Tissue Engineering ,Tissue Scaffolds ,business.industry ,Regeneration (biology) ,musculoskeletal system ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Tendon ,medicine.anatomical_structure ,Ligament ,0210 nano-technology ,business ,Biomedical engineering - Abstract
Tendon and ligament injuries caused by trauma and degenerative diseases are frequent and affect diverse groups of the population. Such injuries reduce musculoskeletal performance, limit joint mobility, and lower people’s comfort. Currently, various treatment strategies and surgical procedures are used to heal, repair, and restore the native tissue function. However, these strategies are inadequate and, in some cases, fail to re-establish the lost functionality. Tissue engineering and regenerative medicine approaches aim to overcome these disadvantages by stimulating the regeneration and formation of neo-tissues. Design and fabrication of artificial scaffolds with tailored mechanical properties are crucial for restoring the mechanical function of tendons. In this review, we present the tendon and ligament structure, their physiology, and performance. On the other hand, we focus on the requirements for the development of an effective reconstruction device. We also describe the most common fiber-based scaffolding systems for tendon and ligament tissue regeneration like strand fibers, woven, knitted, braided, and braid-twisted fibrous structures, as well as electrospun and wet-spun constructs, discussing critically the advantages and limitations of their utilization. Finally, we point out the potential of multi-layered systems as the most effective candidates for tendon and ligaments tissue engineering. The literature of fiber-based systems for tendon and ligament repair, healing and regeneration is reported. Fibrous scaffolds including strand fibers, woven, knitted, braided and braid-twisted fibrous constructs, electrospun and wet-spun structures, as well as multi-layered systems are critically reviewed and described, pointing out advantages and drawbacks of each approach.
- Published
- 2021
26. Smart Drug Delivery: Ultraviolet Light‐Assisted Electrospinning of Core–Shell Fully Cross‐Linked P(NIPAAm‐ co ‐NIPMAAm) Hydrogel‐Based Nanofibers for Thermally Induced Drug Delivery Self‐Regulation (Adv. Mater. Interfaces 12/2020)
- Author
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Yasamin Ziai, Paweł Nakielski, Chiara Rinoldi, Bin Ding, Xiaoran Li, Olga Urbanek, Filippo Pierini, Tomasz Kowalewski, and Sylwia Pawłowska
- Subjects
Core shell ,Materials science ,Targeted drug delivery ,Mechanics of Materials ,Mechanical Engineering ,Nanofiber ,Drug delivery ,Ultraviolet light ,Nanotechnology ,Electrospinning - Published
- 2020
27. Ultraviolet Light‐Assisted Electrospinning of Core–Shell Fully Cross‐Linked P(NIPAAm‐ co ‐NIPMAAm) Hydrogel‐Based Nanofibers for Thermally Induced Drug Delivery Self‐Regulation
- Author
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Tomasz Kowalewski, Sylwia Pawłowska, Yasamin Ziai, Bin Ding, Paweł Nakielski, Chiara Rinoldi, Olga Urbanek, Filippo Pierini, and Xiaoran Li
- Subjects
Core shell ,Materials science ,Targeted drug delivery ,Mechanics of Materials ,Mechanical Engineering ,Nanofiber ,Drug delivery ,Ultraviolet light ,Nanotechnology ,Electrospinning - Published
- 2020
28. Structure and physico-mechanical properties of low temperature plasma treated electrospun nanofibrous scaffolds examined with atomic force microscopy
- Author
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Chiara Rinoldi, Tadeusz Wierzchoń, Ewa Kijeńska, Adrian Chlanda, Wojciech Swieszkowski, and Michał Tarnowski
- Subjects
Scaffold ,Materials science ,Scanning electron microscope ,Atomic force microscopy ,General Physics and Astronomy ,Low temperature plasma ,Plasma treatment ,02 engineering and technology ,Cell Biology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Chemical engineering ,Tissue engineering ,Structural Biology ,General Materials Science ,Wetting ,Adhesive ,0210 nano-technology - Abstract
Electrospun nanofibrous scaffolds are willingly used in tissue engineering applications due to their tunable mechanical, chemical and physical properties. Additionally, their complex openworked architecture is similar to the native extracellular matrix of living tissue. After implantation such scaffolds should provide sufficient mechanical support for cells. Moreover, it is of crucial importance to ensure sterility and hydrophilicity of the scaffold. For this purpose, a low temperature surface plasma treatment can be applied. In this paper, we report physico-mechanical evaluation of stiffness and adhesive properties of electrospun mats after their exposition to low temperature plasma. Complex morphological and mechanical studies performed with an atomic force microscope were followed by scanning electron microscope imaging and a wettability assessment. The results suggest that plasma treatment can be a useful method for the modification of the surface of polymeric scaffolds in a desirable manner. Plasma treatment improves wettability of the polymeric mats without changing their morphology.
- Published
- 2018
29. Naturally derived proteins and glycosaminoglycan scaffolds for tissue engineering applications
- Author
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Marcella Trombetta, Wojciech Święszkowski, Marco Costantini, Nehar Celikkin, Chiara Rinoldi, and Alberto Rainer
- Subjects
0301 basic medicine ,Materials science ,Tissue Engineering ,Tissue Scaffolds ,Inflammatory response ,Natural polymers ,Bioengineering ,Nanotechnology ,Biocompatible Materials ,02 engineering and technology ,Advanced materials ,021001 nanoscience & nanotechnology ,Host tissue ,Regenerative Medicine ,Regenerative medicine ,Biomaterials ,Glycosaminoglycan ,03 medical and health sciences ,030104 developmental biology ,Tissue engineering ,Mechanics of Materials ,Immune reaction ,0210 nano-technology ,Glycosaminoglycans - Abstract
Tissue engineering (TE) aims to mimic the complex environment where organogenesis takes place using advanced materials to recapitulate the tissue niche. Cells, three-dimensional scaffolds and signaling factors are the three main and essential components of TE. Over the years, materials and processes have become more and more sophisticated, allowing researchers to precisely tailor the final chemical, mechanical, structural and biological features of the designed scaffolds. In this review, we will pose the attention on two specific classes of naturally derived polymers: fibrous proteins and glycosaminoglycans (GAGs). These materials hold great promise for advances in the field of regenerative medicine as i) they generally undergo a fast remodeling in vivo favoring neovascularization and functional cells organization and ii) they elicit a negligible immune reaction preventing severe inflammatory response, both representing critical requirements for a successful integration of engineered scaffolds with the host tissue. We will discuss the recent achievements attained in the field of regenerative medicine by using proteins and GAGs, their merits and disadvantages and the ongoing challenges to move the current concepts to practical clinical application.
- Published
- 2016
30. Aligned Cell‐Laden Yarns: Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns (Adv. Healthcare Mater. 7/2019)
- Author
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Cesare Gargioli, Marco Costantini, Marcin Heljak, Ali Khademhosseini, Jacopo Baldi, Monika Ćwiklińska, Ewa Kijeńska-Gawrońska, Jan Guzowski, Robert Buda, Chiara Rinoldi, Stefano Testa, Ersilia Fornetti, Stefano Cannata, and Wojciech Swieszkowski
- Subjects
Biomaterials ,medicine.anatomical_structure ,Materials science ,Cell ,Biomedical Engineering ,medicine ,Pharmaceutical Science ,Stimulation ,Tendon tissue ,Stem cell ,Biomedical engineering - Published
- 2019
31. Tendon Tissue Engineering: Effects of Mechanical and Biochemical Stimulation on Stem Cell Alignment on Cell‐Laden Hydrogel Yarns
- Author
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Stefano Cannata, Stefano Testa, Jan Guzowski, Marcin Heljak, Monika Ćwiklińska, Ewa Kijeńska-Gawrońska, Marco Costantini, Wojciech Swieszkowski, Ali Khademhosseini, Jacopo Baldi, Robert Buda, Chiara Rinoldi, Ersilia Fornetti, and Cesare Gargioli
- Subjects
static mechanical stretching ,Settore BIO/06 ,food.ingredient ,Alginates ,tenogenic differentiation ,Cellular differentiation ,Biomedical Engineering ,Pharmaceutical Science ,Biocompatible Materials ,02 engineering and technology ,010402 general chemistry ,Bone morphogenetic protein ,01 natural sciences ,Gelatin ,Collagen Type I ,Tendons ,Biomaterials ,hydrogel fibers ,food ,Lab-On-A-Chip Devices ,medicine ,Humans ,Fiber ,Cell Proliferation ,Tissue Engineering ,Tissue Scaffolds ,stem cell alignment ,wet spinning ,Chemistry ,Regeneration (biology) ,Settore BIO/13 ,Mesenchymal stem cell ,Cell Differentiation ,Hydrogels ,Mesenchymal Stem Cells ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Tendon ,Collagen Type III ,medicine.anatomical_structure ,Bone Morphogenetic Proteins ,Printing, Three-Dimensional ,Ink ,Stress, Mechanical ,Stem cell ,0210 nano-technology ,Biomedical engineering - Abstract
Fiber-based approaches hold great promise for tendon tissue engineering enabling the possibility of manufacturing aligned hydrogel filaments that can guide collagen fiber orientation, thereby providing a biomimetic micro-environment for cell attachment, orientation, migration, and proliferation. In this study, a 3D system composed of cell-laden, highly aligned hydrogel yarns is designed and obtained via wet spinning in order to reproduce the morphology and structure of tendon fascicles. A bioink composed of alginate and gelatin methacryloyl (GelMA) is optimized for spinning and loaded with human bone morrow mesenchymal stem cells (hBM-MSCs). The produced scaffolds are subjected to mechanical stretching to recapitulate the strains occurring in native tendon tissue. Stem cell differentiation is promoted by addition of bone morphogenetic protein 12 (BMP-12) in the culture medium. The aligned orientation of the fibers combined with mechanical stimulation results in highly preferential longitudinal cell orientation and demonstrates enhanced collagen type I and III expression. Additionally, the combination of biochemical and mechanical stimulations promotes the expression of specific tenogenic markers, signatures of efficient cell differentiation towards tendon. The obtained results suggest that the proposed 3D cell-laden aligned system can be used for engineering of scaffolds for tendon regeneration.
- Published
- 2019
32. Tissue Regeneration: A Multifunctional Polymeric Periodontal Membrane with Osteogenic and Antibacterial Characteristics (Adv. Funct. Mater. 3/2018)
- Author
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Yogendra Kumar Mishra, Sahar Ansari, Tara Aghaloo, Amir Nasajpour, Alireza Moshaverinia, Rainer Adelung, Wojciech Swieszkowski, Chiara Rinoldi, Ali Tamayol, Su Ryon Shin, Afsaneh Shahrokhi Rad, Ali Khademhosseini, and Nasim Annabi
- Subjects
Biomaterials ,Materials science ,Electrochemistry ,Periodontal Membrane ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,0210 nano-technology ,Condensed Matter Physics ,01 natural sciences ,Molecular biology ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials - Abstract
Author(s): Nasajpour, Amir; Ansari, Sahar; Rinoldi, Chiara; Rad, Afsaneh Shahrokhi; Aghaloo, Tara; Shin, Su Ryon; Mishra, Yogendra Kumar; Adelung, Rainer; Swieszkowski, Wojciech; Annabi, Nasim; Khademhosseini, Ali; Moshaverinia, Alireza; Tamayol, Ali
- Published
- 2018
33. A Multifunctional Polymeric Periodontal Membrane with Osteogenic and Antibacterial Characteristics
- Author
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Yogendra Kumar Mishra, Sahar Ansari, Nasim Annabi, Alireza Moshaverinia, Ali Khademhosseini, Su Ryon Shin, Amir Nasajpour, Ali Tamayol, Chiara Rinoldi, Tara Aghaloo, Rainer Adelung, Wojciech Swieszkowski, and Afsaneh Shahrokhi Rad
- Subjects
Periodontitis ,Materials science ,Regeneration (biology) ,Nanoparticle ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease ,Bone tissue ,01 natural sciences ,In vitro ,Electrospinning ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Membrane ,medicine.anatomical_structure ,Electrochemistry ,medicine ,Biophysics ,Periodontal fiber ,Composite material ,0210 nano-technology - Abstract
Periodontitis is a prevalent chronic, destructive inflammatory disease affecting tooth-supporting tissues in humans. Guided tissue regeneration strategies are widely utilized for periodontal tissue regeneration generally by using a periodontal membrane. The main role of these membranes is to establish a mechanical barrier that prevents the apical migration of the gingival epithelium and hence allowing the growth of periodontal ligament and bone tissue to selectively repopulate the root surface. Currently available membranes have limited bioactivity and regeneration potential. To address such challenges, an osteoconductive, antibacterial, and flexible poly(caprolactone) (PCL) composite membrane containing zinc oxide (ZnO) nanoparticles is developed. The membranes are fabricated through electrospinning of PCL and ZnO particles. The physical properties, mechanical characteristics, and in vitro degradation of the engineered membrane are studied in detail. Also, the osteoconductivity and antibacterial properties of the developed membrane are analyzed in vitro. Moreover, the functionality of the membrane is evaluated with a rat periodontal defect model. The results confirmed that the engineered membrane exerts both osteoconductive and antibacterial properties, demonstrating its great potential for periodontal tissue engineering.
- Published
- 2017
34. Producing and characterizing PCL/PA6 three dimensional nanofibrous scaffolds for tendons and ligaments regeneration
- Author
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Chiara, Rinoldi, primary, Ewa, Kijenska, additional, and Wojciech, Swieszkowski, additional
- Published
- 2016
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