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Abstract During oviposition, ectoparasitoid wasps not only inject their eggs but also a complex mixture of proteins and peptides (venom) in order to regulate the host physiology to benefit their progeny. Although several endoparasitoid venom proteins have been identified, little is known about the components of ectoparasitoid venom. To characterize the protein composition of Torymus sinensis Kamijo (Hymenoptera: Torymidae) venom, we used an integrated transcriptomic and proteomic approach and identified 143 venom proteins. Moreover, focusing on venom gland transcriptome, we selected additional 52 transcripts encoding putative venom proteins. As in other parasitoid venoms, hydrolases, including proteases, phosphatases, esterases, and nucleases, constitute the most abundant families in T. sinensis venom, followed by protease inhibitors. These proteins are potentially involved in the complex parasitic syndrome, with different effects on the immune system, physiological processes and development of the host, and contribute to provide nutrients to the parasitoid progeny. Although additional in vivo studies are needed, initial findings offer important information about venom factors and their putative host effects, which are essential to ensure the success of parasitism.

The pine processionary moth (PPM), Thaumetopoea pityocampa (Denis and Schiffermüller), is one of the most economically important forest defoliators in southern Europe. This pest is a univoltine oligophagous insect species, and the genus Pinus represents its main host. Investigations were carried out in the five-year period 2016–2020 in NW Italy. PPM males were monitored using commercial funnel traps baited with sex pheromone. The infestation index was recorded by counting the number of nests per tree. Temperature and rainfall were automatically recorded by 94 georeferenced meteorological stations. Adult presence was evaluated as the maximum number of captured individuals in a day, total captures during the season, the Julian day at max captures, or at first or last captures. Environmental variables (altitude, cumulative rain, and cumulative degree-days) and biological parameters were summarized using principal component analysis. Our study showed that the analyzed variables contribute to driving and affecting the PPM population dynamics, which also exhibited a year-to-year decrease. Due to the environmental and sanitary importance, all the data collected about the PPM will be useful to develop predictive risk models, as to deploy countermeasures in a timely and cost-effective manner.

(1) Torymus sinensis, the biocontrol agent of the Asian chestnut gall wasp Dryocosmus kuriphilus, is univoltine, but in NW Italy a small percentage of individuals exhibits a prolonged diapause, mainly as late instar larva. (2) In 2020, the diapause was investigated to evaluate its trend over the years. Due to the low survival rate of diapausing T. sinensis adults, the seasonal variation in the galls’ toughness was evaluated, thus assuming that dry galls over time can negatively affect emergence. The window of vulnerability of the gall wasp galls was also evaluated in controlled conditions. (3) The results showed that the average number of second year T. sinensis emerging per 100 cells was 0.41 ± 0.05, and dead adults accounted for 4.1 ± 0.23 per 100 cells. Gall toughness resulted in lower values for galls collected in May and June, and then gradually increased over time. In general, no difference was detected in the wall toughness of galls formed during the previous year when compared to current-year dry galls. Oviposition was recorded on all the tested galls collected in May and June, and no difference in the number of oviposition events was detected. Conversely, no oviposition was observed in July. Comparing the number of oviposition events by T. sinensis and the gall toughness, a negative correlation was found (R2 = −0.99). (4) The present findings contribute descriptive information on this gall’s structural traits, and the influence on gall wasp management is also discussed.

Bark beetle outbreaks are responsible for the loss of large areas of forests and in recent years they appear to be increasing in frequency and magnitude as a result of climate change. The aim of this study is to develop a new standardized methodology for the automatic detection of the degree of damage on single fir trees caused by bark beetle attacks using a simple GIS-based model. The classification approach is based on the degree of tree canopy defoliation observed (white pixels) in the UAV-acquired very high resolution RGB orthophotos. We defined six degrees (categories) of damage (healthy, four infested levels and dead) based on the ratio of white pixel to the total number of pixels of a given tree canopy. Category 1: 75% (dead). The definition of “white pixel” is crucial, since light conditions during image acquisition drastically affect pixel values. Thus, whiteness was defined as the ratio of red pixel value to the blue pixel value of every single pixel in relation to the ratio of the mean red and mean blue value of the whole orthomosaic. The results show that in an area of 4 ha, out of the 1376 trees, 277 were healthy, 948 were infested (Cat 2, 628; Cat 3, 244; Cat 4, 64; Cat 5, 12), and 151 were dead (Cat 6). The validation led to an average precision of 62%, with Cat 1 and Cat 6 reaching a precision of 73% and 94%, respectively.

(1) The box tree moth (BTM), Cydalima perspectalis Walker, represents one of the recent examples of exotic insect pests native to Asia accidentally introduced in Europe by the nursery trade. In Europe, BTM develops on Buxus sempervirens L., causing significant damage to ornamental and natural plants. (2) Basic aspects of BTM biology were investigated at three sites in the NW Alps, where B. sempervirens occurs on rocky slopes, forming a protected habitat. In 2019–2021, we evaluated: (i) the seasonal flight activity with sex pheromone traps, (ii) the fecundity and the effects of food on the egg load, (iii) the potential adaptation of BTM on native secondary host plants, and (iv) the recruitment of natural enemies. (3) Our results revealed the presence of two generations per year. The number of mature eggs increased in fed adults and was higher in females of the first generation. No evidence of BTM shift on secondary plants was found in either laboratory or natural conditions, and no significant occurrence of natural enemies was recorded, except for one pupa of the tachinid Pseudoperichaeta nigrolineata. (4) Our findings provide valuable information to address and timely plan management strategies to preserve B. sempervirens as an essential component of rare forest ecosystems.

(1) Background: Pheromone-based devices are successfully used to control insect pests in agriculture. (2) Methods: Investigations were conducted to evaluate the effectiveness of mating disruption (MD) to control the chestnut tortrix moths, Cydia fagiglandana and Cydia splendana. Surveys were performed in northern Italy in 2019–2020. MD was carried out using the pheromone dispenser Ecodian® CT. The effectiveness of MD was assessed by recording male adult catches in pheromone-baited sticky traps and larvae in chestnut fruits, comparing MD and control plots. (3) Results: The total number of trapped males was significantly lower in MD plots than in control ones, for all sites and years. Trap catch suppression in MD plots averaged 89.5% and 93.8% for C. fagiglandana and 57.4% and 81% for C. splendana in 2019 and 2020, respectively. The larval infestation rate in fruits did not vary between plots except for one site where a reduction of about 71% in the MD plot was recorded in 2019. (4) Conclusions: Low catches in MD plots turned out to be a good measure of the effectiveness of communication disruption, but no satisfactory data have been obtained regarding fruit infestation, highlighting how the reduction of male catches cannot always be considered as a reliable indicator of successful control. Specific investigations about background population density, dispersal and mating/oviposition behavior are thus essential for a viable management strategy.

The EU has long-recognised the functions and contributions of beekeeping in sustainable rural area development. In 2018, the EU adopted the Pollinator Initiative to strengthen its pollinator conservation policies. To support the design of effective rural development actions, this work describes and tests an easy-to-apply, mixed-method tool for use with SWOT analysis. A two-step methodology was trialled with beekeepers in Piedmont Region (NW Italy). In step one, two independent groups of beekeepers operating in separate protected and intensive agricultural areas completed a SWOT matrix. In step two, three expert panels (beekeeper association leaders, honey market organisation leaders, and entomologists) prioritised the effects of the SWOT items with a quantitative weighting and rating process. Results suggest that the sector needs better-targeted incentives and that ‘soft’ policies on extension, advisory, and institutional measures could play a relevant role. The method was also confirmed as suitable for use with non-expert evaluators, such as policy officers and practitioners.

(1) Background: Bees are the primary animal pollinators in most ecosystems, and honey bees (Apis mellifera L.) are important providers of pollination ecosystem services and products. Climate change is one of the major threats for honey bees. (2) Objectives and methods: Qualitative research using focus group discussions was carried out in northwestern Italy, to investigate the beekeepers’ perceptions of climate change effects, the relevant management adaptations, and the main issues affecting the sector. (3) Results: Beekeepers reported several consequences related to severe weather events (weakening or loss of colonies; scarcity of nectar, pollen, and honeydew; decrease or lack of honey and other bee products; greater infestation by varroa; decline in pollination), making it necessary to provide supplemental sugar feeding, intensive transhumance, more effective and sustainable techniques for varroa control, and increased production of nuclei. A strengths, weaknesses, opportunities, and threats (SWOT) analysis was completed, displaying the factors able to strengthen or weaken the resilience of the beekeeping sector to climate change. (4) Conclusions: Thanks to their strong motivation and collaborative attitude, beekeepers succeed in adopting farm and bee hive adaptation strategies that are able to limit the climatic adverse effects. However, these findings highlight how the institutional and financial support for the beekeeping sector should be strengthened and better targeted.

The pine processionary moth (PPM), Thaumetopoea pityocampa (Denis and Schiffermüller, 1775) (Lepidoptera, Notodontidae), is considered one of the main insect defoliators of conifers in Southern Europe and North Africa. The species is oligophagous on pines and cedars in Mediterranean countries. This 6-year investigation (2014–2019), carried out in Aosta Valley (NW Italy), on Pinus sylvestris L. aimed to: (i) Monitor the PPM population with pheromone-baited funnel traps; (ii) assess the infestation index (0–5 classes) according to the degree of defoliation; and (iii) apply control strategies, namely Bacillus thuringiensis var. kurstaki (Btk) and mating disruption (MD). In total, 9618 ha were visually monitored and assigned an infestation index. The percentage of woodland stands that were strongly defoliated by PPM (infestation index ≥ 3) increased progressively between 2015 and 2016, affecting from 8% to 19% of the total area monitored; this area decreased to 16% and 13% in 2017 and 2018, respectively, followed by an abrupt decline to 4% in 2019. Both Btk applications and mating disruption significantly reduced the infestation. Where Btk was applied, the rate of larval mortality ranged from 79.47% to 98.43%; conversely, in the control plots, the larval mortality was, on average, 1.56%. The mean number of PPM males captured in traps was significantly lower in the plots where MD was performed, ranging from 8.36 ± 2.37 to 13.47 ± 4.68. The mean number of males captured in the control plots was, on average, 119.16 ± 12.68. The total number of nests recorded per tree was significantly lower in MD plots, ranging from 0.41 ± 0.05 to 0.94 ± 0.14. In the control plots, there were, on average, 4.37 ± 0.76 nests per tree. As already documented for several defoliating forestry insect pests, both Btk and mating disruption proved effective in controlling PPM infestations, and thus, microbial insecticides should be strongly encouraged and adopted by forest managers.

(1) Background: Pammene fasciana (L.), Cydia fagiglandana (Zeller), and C. splendana (Hübner) (Lepidoptera: Tortricidae) are considered key moth pests of chestnut in Europe. (2) Methods: Investigations were performed in 2018–2019 in northern Italy. Sticky traps and commercially available pheromones were used for monitoring; moreover, two experimental pheromone blends were tested. All specimens were identified according to male genitalia and molecular analyses. Newly formed chestnut husks and fruits were randomly collected to evaluate the presence of larvae and/or feeding damage, by comparing it to trap catches. (3) Results: P. fasciana was present in all the sites, whereas Cydia species were recorded in three sites of six, with differences in abundance related to pheromone blends studied. Several non-target species, such as Oegoconia novimundi (Busck) and Cydia ilipulana (Walsingham), were present. Data about the seasonal flight activity are provided. (4) Conclusions: This research contributes to ascertaining the presence and abundance of tortrix moths in Italian chestnut groves, and the presence of non-target species highlights the risk of overestimating catches. Fruit damage recorded did not always reflect catches made by pheromone traps, suggesting that monitoring may underestimate the real size of moths’ populations. All of the data acquired are important for planning specific control measures.