1. Matrix Protein 2 Vaccination and Protection against Influenza Viruses, Including Subtype H5N1.
- Author
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Tompkins, Stephen Mark, Zi-Shan Zhao, Chia-Yunb Lo, Misplon, Julia A., Liu, Teresa, Zhiping Ye, Hogan, Robert J., Zhengqi Wu, Benton, Kimberly A., Tumpey, Terrence M., and Epstein, Suzanne L.
- Subjects
VACCINATION ,EXTRACELLULAR matrix proteins ,INFLUENZA A virus ,INFLUENZA viruses ,PEPTIDES ,IMMUNOGLOBULINS ,ADENOVIRUSES ,T cells - Abstract
Changes in influenza viruses require regular reformulation of strain-specific influenza vaccines. Vaccines based on conserved antigens provide broader protection. Influenza matrix protein 2 (M2) is highly conserved across influenza A subtypes. To evaluate its efficacy as a vaccine candidate, we vaccinated mice with M2 peptide of a widely shared consensus sequence. This vaccination induced antibodies that cross-reacted with divergent M2 peptide from an H5N1 subtype. A DNA vaccine expressing full-length consensus-sequence M2 (M2-DNA) induced M2-specific antibody responses and protected against challenge with lethal influenza. Mice primed with M2-DNA and then boosted with recombinant adenovirus expressing M2 (M2-Ad) had enhanced antibody responses that cross-reacted with human and avian M2 sequences and produced T-cell responses. This M2 prime-boost vaccination conferred broad protection against challenge with lethal influenza A, including an H5N1 strain. Vaccination with M2, with key sequences represented, may provide broad protection against influenza A. [ABSTRACT FROM AUTHOR]
- Published
- 2007
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