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A 6-year-old intact male Jindo dog was presented for reduced appetite, depression, and tympanic abdominal distension. Abdominal radiographs showed severe pneumoperitoneum and an intestinal mass. Computed tomography revealed massive pneumoperitoneum associated with intestinal perforation, with cervical and thoracoabdominal wall emphysema, pneumomediastinum, pneumoretroperitoneum, pneumoscrotum, and peritonitis. Surgery confirmed a ruptured jejunal mass, and histopathologic evaluation of the excised tissues revealed intestinal lymphoma. Clinicians should therefore consider the possibility of gastrointestinal perforation in patients with severe pneumoperitoneum even where there is no history of trauma. Despite clinical stability, intensive monitoring and urgent decompressive intervention should be performed in patients with severe pneumoperitoneum.

This study was performed to evaluate the effect of intra‐abdominal pressure (IAP) on intraocular pressure (IOP) in conscious dog models using a balloon technique to generate intra‐abdominal hypertension. Six healthy dogs without ocular abnormalities were evaluated in this study. A balloon device was placed in the intra‐abdominal cavity. The abdomen was insufflated to IAP levels of 15 and 25 mmHg using the balloon device. Intraocular pressure was measured at baseline, at IAP levels of 15 and 25 mmHg, and after decompression. In comparison with the mean baseline IOP (15.1 ± 2.0 mmHg), there was a significant increase in IOP at IAP levels of 15 mmHg (20.0 ± 2.1 mmHg) and 25 mmHg (19.9 ± 2.2 mmHg), corresponding to a 32.4% and 31.7% increase from baseline IOP, respectively. The mean IOP after decompression (14.8 ± 1.7 mmHg) was significantly lower compared to those at IAP levels of 15 and 25 mmHg. The present findings demonstrate that increased IAP has a clinically significant effect on IOP in dogs under conscious conditions. Although more research is needed to determine of increased IAP on IOP, these findings suggest that increased IAP leads to mild and reversible increase in IOP.

Objective To describe an approach for ethmoidal nerve block (EBLOCK) and to compare the effects of a maxillary nerve block (MBLOCK), EBLOCK and their combination (M-EBLOCK) on heart rate (HR), systolic (SAP), mean (MAP), diastolic (DAP) arterial pressures and respiratory rate (fR) during nasal stimulation in dogs. Study design Prospective, blinded, randomized, crossover placebo-controlled study. Animals Beagle dogs (five cadavers, nine live dogs), with a median (interquartile range) weight of 10.5 (10.3–11.0) kg. Methods The accuracy of iohexol injections (each 1 mL) at the maxillary and ethmoidal foramina in cadavers was evaluated using computed tomography. Then, anesthetized dogs were administered four bilateral treatments separated by 1 week, saline or 2% lidocaine 1 mL per injection: injections of saline at the maxillary and ethmoidal foramina (Control), injections of lidocaine at the maxillary foramina and saline at the ethmoidal foramina (MBLOCK), injections of saline at the maxillary foramina and lidocaine at the ethmoidal foramina (EBLOCK) and injections of lidocaine at all foramina (M-EBLOCK). The ventral nasal meatus was bilaterally stimulated using cotton swabs, and HR, SAP, MAP, DAP and fR were continuously recorded. Values for each variable were compared before and after stimulation using Wilcoxon signed-rank test. Changes in variables among treatments were analyzed using Mann–Whitney U and Kruskal–Wallis tests (p ≤ 0.05). Results Computed tomography revealed iohexol distribution around the openings of the target foramina in all cadavers. In living dogs, HR, SAP, MAP, DAP and fR significantly increased after stimulation within each treatment (p Conclusions and clinical relevance Concurrent injections of lidocaine at the maxillary and ethmoidal foramina attenuated HR, arterial pressure and fR responses to nasal stimulation in Beagle dogs.

Objective To evaluate endotracheal tube intracuff pressure (Pcuff) changes over time and the effect of these changes on air leak pressure (Pleak). Study design Prospective experimental study. Animals A group of nine healthy adult Beagle dogs. Methods In part I, in vitro measurements of Pcuff were recorded for 1 hour in eight endotracheal tubes subjected to four treatments: room temperature without lubricant (RT0L), room temperature with lubricant (RTWL), body temperature without lubricant (BT0L), and body temperature with lubricant (BTWL). In part II, nine dogs were endotracheally intubated and Pleak was evaluated at Pcuff of 25 mmHg. Subsequently, Pcuff was reset to 25 mmHg (baseline) and Pcuff measurements were recorded every 5 minutes for 1 hour. Subsequently, a second Pleak measurement was recorded at the current Pcuff. The data were analyzed using Wilcoxon signed-rank test, repeated measures anova and Mann–Whitney U test. Results In part I, Pcuff differed significantly between the RT0L and RTWL treatments at 5–60 minutes, and between the BT0L and BTWL treatments at 5–35, 55 and 60 minutes (p Conclusions and clinical relevance Significant decreases in Pcuff over time were measured. Pleak may decrease during anesthesia and increase the risk for silent pulmonary aspiration. The results indicate the need for testing Pcuff more than once, especially at 10 minutes after the onset of anesthesia.

OBJECTIVE To evaluate a new balloon technique to induce intra-abdominal hypertension (IAH) and abdominal compartment syndrome in a conscious dog model, and to evaluate the effect of intra-abdominal pressure (IAP) on cardiovascular, respiratory, and arterial blood gas values in conscious dogs with IAH. DESIGN Prospective, experimental study. SETTING University-based small animal research facility. ANIMALS Six healthy Beagle dogs, 4 males, and 2 females. INTERVENTIONS A new balloon device designed for this study using a Foley urethral catheter and latex balloon was placed in the abdominal cavity. Consecutive measurements of IAP were made by measuring the intravesicular pressure. The abdomen was inflated with air to IAPs of 10, 15, 20, and 25 mm Hg. Heart rate, respiratory rate, systolic arterial blood pressure, and arterial blood gases were evaluated at baseline and at 15, 30, 45, 60, 120, 240, and 300 minutes after IAP increase. MEASUREMENTS AND MAIN RESULTS The air insufflated into the intra-abdominal balloon device significantly increased the IAP and led to sustained IAH. The respiratory rate increased significantly (P < 0.05) when IAP was increased to 15, 20, and 25 mm Hg. Although heart rate, systolic arterial blood pressure, PaO2 , and PaCO2 did not show statistically significant differences between baseline and posttreatment values over time, the dogs with increased IAP showed a distended abdomen and apparent discomfort, and 4/6 (67%) vomited. After measurement of IAP, air was removed. There were no adverse effects noted after removal of the balloon device. CONCLUSION The balloon device was successfully insufflated and led to sustained IAH in conscious dogs. This balloon technique does not require general anesthesia for instillation or removal of gas after installment. An acute IAP increase in normal conscious dogs induced discomfort, vomiting, and increased respiratory effort.

To evaluate an approach to the canine lumbar dorsal root ganglion (DRG), a significant contributor to the pain pathway, using new methylene blue staining.Prospective randomized study.A total of three Beagle dog cadavers weighing 10.4 ± 0.7 kg (mean ± standard deviation).Bilateral third to fifth lumbar DRG approaches were performed in three dog cadavers positioned in sternal recumbency. The mammillary process was palpated, and a 22 gauge spinal needle was inserted through the skin 1 cm lateral to the process and directed towards the median plane at a 45° angle to the dorsal plane. The needle was advanced along the transverse plane until touching bone, or a popping sensation was detected. Under fluoroscopic guidance, the position of the needle tip was adjusted to be in the cranioventral part of the intervertebral foramen. The location of the needle was confirmed by demarcation of the nerve roots after iohexol (0.1 mL) injection. For evaluation of the DRG approach, new methylene blue (0.1 mL) was injected. Subsequently, anatomical dissection of the area was performed. The DRG staining was scored as follows: 0, no staining; 1, partial (50%); 2, partial (≥50%); and 3, complete staining. Comparisons among the staining scores of the third to fifth DRG were assessed with the Friedman test.Staining score 3 was achieved in 14 of 18 (77.8%) sites. Staining scores 2, 1 and 0 were identified at two, one and one of the 18 sites, respectively. No significant difference was noted in the staining scores among the third to fifth DRGs (p = 0.78).The technique used for DRG injections achieved adequate DRG staining, supporting use of the fluoroscopy-guided approach to the canine lumbar DRG.

Objective To compare the effects of epidural injection of three volumes of lidocaine injected at the third (T3) or eleventh thoracic vertebra (T11) in conscious dogs to induce thoracic epidural anesthesia (TEA) and to measure the epidural dispersion of iohexol under similar conditions. Study design Prospective crossover experiment. Animals A group of five Beagle dogs weighing 10.4 ± 0.5 kg (mean ± standard deviation). Methods Each dog was anesthetized twice, separated by 1 week, for inserting an epidural catheter at the lumbosacral space and advancing the tip to T3 (treatment TEAT3) or T11 (treatment TEAT11). For each treatment, three volumes of 2% lidocaine (0.05, 0.10 and 0.20 mL kg–1) were administered at 24 hour intervals, and sensory blockade (SB) of dermatomes was estimated by pinching the skin with mosquito forceps. Under identical conditions of injection volume and site, iohexol was administered 3 hours after lidocaine injection to identify epidural distribution (ED) using computed tomography. The effects of injection site and volume on SB of thoracic dermatomes and ED were analyzed using a linear mixed model (p Results Thoracic SB and ED significantly increased as the volume increased (p Conclusions and clinical relevance TEA induced at T3 produced less thoracic SB than did TEA at T11 with the same volumes of lidocaine. The cervical SB obtained with the highest volume of lidocaine may increase the risk of laryngeal paralysis and pulmonary aspiration.

Objective To determine the optimal endotracheal tube size in Beagle dogs using thoracic radiography. Study design Prospective, randomized, crossover experimental study. Animals A total of eight healthy adult Beagle dogs. Methods Lateral thoracic radiographs were used to measure the internal tracheal diameter at the thoracic inlet. This measurement was multiplied by 60, 70 and 80% to determine the outer diameter of the endotracheal tube for each dog. In each treatment, medetomidine (5 μg kg−1) was administered intravenously (IV) for premedication. Anesthesia was induced with alfaxalone (2 mg kg−1) IV and maintained with isoflurane. After induction of anesthesia, the resistance to passage of the endotracheal tube through the trachea was scored by a single anesthesiologist. Air leak pressures (Pleak) were measured at intracuff pressures (Pcuff) of 20 and 25 mmHg (27 and 34 cmH2O). The results were analyzed using Friedman tests and repeated measures anova . Results There were statistically significant increases in resistance as the endotracheal tube size increased (p = 0.003). When Pcuff was 20 mmHg, mean Pleak for the 60, 70 and 80% treatments were 9.7 ± 6.7, 16.2 ± 4.2 and 17.4 ± 3.9 cmH2O, respectively, but no significant differences were found. When Pcuff was 25 mmHg, mean Pleak for the 60, 70 and 80% treatments were 10.6 ± 8.5, 19.7 ± 4.9 and 20.8 ± 3.6 cmH2O, respectively, and statistically significant increases were found between treatments 60 and 70% (p = 0.011) and between treatments 60 and 80% (p = 0.020). Three dogs in the 80% treatment had bloody mucus on the endotracheal tube cuff after extubation. Conclusions and clinical relevance Results based on resistance to insertion of the endotracheal tube and the ability to achieve an air-tight seal suggest that an appropriately sized endotracheal tube for Beagle dogs is 70% of the internal tracheal diameter measured on thoracic radiography.

To evaluate a new balloon technique to induce intra-abdominal hypertension (IAH) and abdominal compartment syndrome in a conscious dog model, and to evaluate the effect of intra-abdominal pressure (IAP) on cardiovascular, respiratory, and arterial blood gas values in conscious dogs with IAH.Prospective, experimental study.University-based small animal research facility.Six healthy Beagle dogs, 4 males, and 2 females.A new balloon device designed for this study using a Foley urethral catheter and latex balloon was placed in the abdominal cavity. Consecutive measurements of IAP were made by measuring the intravesicular pressure. The abdomen was inflated with air to IAPs of 10, 15, 20, and 25 mm Hg. Heart rate, respiratory rate, systolic arterial blood pressure, and arterial blood gases were evaluated at baseline and at 15, 30, 45, 60, 120, 240, and 300 minutes after IAP increase.The air insufflated into the intra-abdominal balloon device significantly increased the IAP and led to sustained IAH. The respiratory rate increased significantly (P0.05) when IAP was increased to 15, 20, and 25 mm Hg. Although heart rate, systolic arterial blood pressure, PaOThe balloon device was successfully insufflated and led to sustained IAH in conscious dogs. This balloon technique does not require general anesthesia for instillation or removal of gas after installment. An acute IAP increase in normal conscious dogs induced discomfort, vomiting, and increased respiratory effort.

Somatic cell nuclear transfer allows generation of genetically identical animals using donor cells derived from animals with particular traits. To date, few studies have investigated whether or not these cloned dogs will show identical behavior patterns. To address this question, learning, memory and exploratory patterns were examined using six cloned dogs with identical nuclear genomes. The variance of total incorrect choice number in the Y-maze test among cloned dogs was significantly lower than that of the control dogs. There was also a significant decrease in variance in the level of exploratory activity in the open fields test compared to age-matched control dogs. These results indicate that cloned dogs show similar cognitive and exploratory patterns, suggesting that these behavioral phenotypes are related to the genotypes of the individuals.