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Isolates of three endornavirus species were identified co-infecting an unidentified species of Ceratobasidium, itself identified as a symbiont from within the roots of a wild plant of the terrestrial orchid Pterostylis vittata in Western Australia. Isogenic lines of the fungal isolate lacking all three mycoviruses were derived from the virus-infected isolate. To observe how presence of endornaviruses influenced gene expression in the fungal host, we sequenced fungus-derived small RNA species from the virus-infected and virus-free isogenic lines and compared them. The presence of mycoviruses influenced expression of small RNAs. Of the 3272 fungus-derived small RNA species identified, the expression of 9.1% (300 of 3272) of them were up-regulated, and 0.6% (18 of 3272) were down-regulated in the presence of the viruses. Fourteen novel micro-RNA-like RNAs (Cer-milRNAs) were predicted. Gene target prediction of the differentially expressed Cer-milRNAs was quite ambiguous; however, fungal genes involved in transcriptional regulation, catalysis, molecular binding, and metabolic activities such as gene expression, DNA metabolic processes and regulation activities were differentially expressed in the presence of the mycoviruses.

The 2008 Wenchuan earthquake destroyed large areas of vegetation. Presently, these areas of damaged vegetation are at various stages of recovery. In this study, we present a probabilistic approach for slope stability analysis that quantitatively relates data on earthquake-damaged vegetation with slope stability in a given river basin. The Mianyuan River basin was selected for model development, and earthquake-damaged vegetation and post-earthquake recovery conditions were identified via the normalized difference vegetation index (NDVI), from multi-temporal (2001-2014) remote sensing images. DSAL (digital elevation model, slope, aspect, and lithology) spatial zonation was applied to characterize the survival environments of vegetation, which were used to discern the relationships between successful vegetation regrowth and environmental conditions. Finally, the slope stability susceptibility model was trained through multivariate analysis of earthquake-damaged vegetation and its controlling factors (i.e. topographic environments and material properties). Application to the Subao River basin validated the proposed model, showing that most of the damaged vegetation areas have high susceptibility levels (88.1%>susceptibility level 3, and 61.5%>level 4). Our modelling approach may also be valuable for use in other regions prone to landslide hazards. [ABSTRACT FROM AUTHOR]

The 2008 Wenchuan earthquake-induced landslides destroyed larger areas of mountain vegetation and produced large volume of landslide-debris, which made the vegetation's hydrological adjusting function diminished and made the hydrological progresses in slopes changed, resulting in severe erosion and catastrophic debris flows for a rather long time. Since 2008, the landslide-damaged vegetation and its hydrological function have been recovering. In this paper, the Minjiang Upstream watersheds around Yingxiu Town were selected. First, the landslide-damaged vegetation was identified and monitored via multi-temporal (2001-2014) satellite images. Then, the slope materials stabil ity was assessed through topographic analysis of the vegetation survival environments. Then, the hydrological connectivity index (HCI) was defined to describe the upstream sediment production and downstream transport pathway. Finally, results indicated that HCI decreased annually with the vegetation recovery after the obvious increases during the earthquakes. While, analysis of 2008-2013 debris flow events indicated that the areas, the vertical drop to river<1000m and the horizontal distance to river<2500m, have high HCI increases and are more susceptible for debris flow formation. Monitoring the landslide-damaged vegetation recovery processes can contribute to assess the hydrological connectivity changes and understand the debris flow formation. [ABSTRACT FROM AUTHOR]

Introduction: The purpose of this study was to analyze the risk factors affecting precancerous lesions, and cancer of oral cavity, and to assess efficacy of visual screening for oral mucosal lesions., Methods: The medical records of patients older than 30 years of age with history of habitual cigarette smoking or betel quid chewing that received screening for oral mucosal lesions between January 2012 and December 2012 were retrospectively reviewed. The patients' age, gender, risk factors, screening findings, and histopathology results of biopsy were included for further analysis., Results: A total of 1341 patients were enrolled in this study. There were 1080 males and 261 females ranging from 30 to 96 years of age, with a mean age of 53.9±13.6 years. After screening, 226 (16.9%) were found to be positive of oral lesions. Among these 226 patients, 69 (30.5%) underwent biopsy under local anesthesia, and the histopathology showed malignancy in 13 (5.8%). All of the confirmed malignant cases were squamous cell carcinoma. Among them, 12 received further staging examination and one was lost to follow-up resulting in unknown stage. The early stage oral cavity cancer (stage I and II) accounted for 84.6% (11/13)., Conclusions: The detection rate of early stage oral cavity cancer in our study was reasonable. Therefore, visual screening for oral cavity cancer is recommended for patients with habitual cigarette smoking or betel quid chewing.

Problem/objective: Acute suppurative sialadenitis commonly affects the parotid gland. However, acute suppurative parotitis with abscess formation is less common and possibly complicated by deep neck space infection and sepsis. Our aim was to analyze the clinical features, radiological findings, treatment modalities, and microbiology of parotid abscesses treated at a regional hospital in Taiwan over a 15-year period., Methods: Records from patients diagnosed with acute suppurative parotitis or parotid abscesses between January 1998 and December 2012 were retrospectively reviewed. Parotid abscesses were confirmed by computed tomography (CT) examinations., Results: Fourteen patients (9 males; mean age+standard deviation, 49.6 +/- 14.4 [range, 22-75] years) with parotid abscesses were included. Painful swelling at the angle of the jaw was the most common symptom. All patients sustained unilateral parotid abscesses, with left-sided lesions in 10 patients, and right-sided ones in 4 patients. Radiographically, 13 of 14 abscess lesions were located in the superficial lobe, and 1 was in the deep lobe of the parotid gland. Besides sufficient fluid hydration, maintenance of good oral hygiene, and administration of parenteral antibiotics, all patients were treated with surgical incision and drainage. Klebsiella pneumoniae was the organism most commonly isolated from abscess cultures., Conclusions: Parotid abscesses were found in about one fifth of patients with acute infectious parotid disease. Parenteral antibiotics plus surgical incision and drainage was the treatment of choice.