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195 results on '"Cherbut, C."'

2. Butyrate specifically modulates MUC gene expression in intestinal epithelial goblet cells deprived of glucose

Author

Gaudier, E., Jarry, A., Blottiere, H.M., de Coppet, P., Buisine, M.P., Aubert, J.P., Laboisse, C., Cherbut, C., and Hoebler, C.

Subjects
Gastric mucosa -- Research, Fatty acids -- Research, Biological sciences
Abstract

The mucus layer covering the gastrointestinal mucosa is considered the first line of defense against aggressions arising from the luminal content. It is mainly composed of high molecular weight glycoproteins called mucins. Butyrate, a short-chain fatty acid produced during carbohydrate fermentation, has been shown to increase mucin secretion. The aim of this study was to test 1) whether butyrate regulates the expression of various MUC genes, which are coding for protein backbones of mucins, and 2) whether this effect depends on butyrate status as the major energy source of colonocytes. Butyrate was provided at the apical side of human polarized colonic goblet cell line HT29-Cl.16E in glucose-rich or glucose-deprived medium. In glucose-rich medium, butyrate significantly increased MUC3 and MUC5B expression (1.6-fold basal level for both genes), tended to decrease MUC5AC expression, and had no effect on MUC2 expression. In glucose-deprived medium, i.e., when butyrate was the only energy source available, MUC3 and MUC5B increase persisted, whereas MUC5AC expression was significantly enhanced (3.7-fold basal level) and MUC2 expression was strikingly increased (23-fold basal level). Together, our findings show that butyrate is able to upregulate colonic mucins at the transcriptional level and even better when it is the major energy source of the cells. Thus the metabolism of butyrate in colonocytes is closely linked to some of its gene-regulating effects. The distinct effects of butyrate according to the different MUC genes could influence the composition and properties of the mucus gel and thus its protective function. mucin; short-chain fatty acids; energy source; human colonic cell line

Published
2004

7. Role of gastrointestinal motility in the delay of absorption by dietary fiber

Author

Cherbut, C.

Subjects
Gastrointestinal system -- Motility, Fiber in human nutrition -- Physiological aspects
Abstract

The fluid viscosity in the stomach and small intestine is enhanced due to viscous soluble fiber, and the luminal volume is expanded because of insoluble, discrete fiber particles. The gastrointestinal motility and so the intraluminal movements affect the glucose absorption. The deceleration in glucose absorption by the dietary fiber is possible through restriction of the intraluminal movements, by raising the resistance of the contents or by lowering the proportion of mixing to propulsive intestinal contractions.

Published
1995

24. Functional mapping of NPY/PYY receptors in rat and human gastro-intestinal tract

Author

Ferrier, L., Segain, Jp, Bonnet, C., Cherbut, C., Lehur, Pa, Jarry, A., Galmiche, Jp, Hervé BLOTTIERE, Unité de recherche Fonctions Digestives et de Nutrition Humaines (UFDNH), and Institut National de la Recherche Agronomique (INRA)

Subjects
PEPTIDE YY, [SDV]Life Sciences [q-bio], RAT, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2002

25. Short-chain fatty acids induce cytoskeletal and extracellular protein modifications associated with modulation of proliferation on primary culture of rat intestinal smooth muscle cells

Author

Le Blay, G., Hervé BLOTTIERE, Ferrier, L., Le Foll, E., Bonnet, C., Galmiche, Jp, Cherbut, C., Unité de recherche Fonctions Digestives et de Nutrition Humaines (UFDNH), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], RAT, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2000

26. Probiotic modulation of symbiotic gut microbial-host metabolic interactions in a humanized microbiome mouse model

Author

Martin, F.P.J., Wang, Y., Sprenger, N., Yap, K.S., Rezzi, S., Ramadan, Z., Peré-Trepat, E., Rochat, F., Cherbut, C., van Bladeren, P.J., Fay, L.B., Kochhar, S., LindOn, J.C., Holmes, E., Nicholson, J.K., Martin, F.P.J., Wang, Y., Sprenger, N., Yap, K.S., Rezzi, S., Ramadan, Z., Peré-Trepat, E., Rochat, F., Cherbut, C., van Bladeren, P.J., Fay, L.B., Kochhar, S., LindOn, J.C., Holmes, E., and Nicholson, J.K.

Abstract

The transgenomic metabolic effects of exposure to either Lactobacillus paracasei or Lactobacillus rhamnosus probiotics have been measured and mapped in humanized extended genome mice (germ-free mice colonized with human baby flora). Statistical analysis of the compartmental fluctuations in diverse metabolic compartments, including biofluids, tissue and cecal short-chain fatty acids (SCFAs) in relation to microbial population modulation generated a novel top-down systems biology view of the host response to probiotic intervention. Probiotic exposure exerted microbiome modification and resulted in altered hepatic lipid metabolism coupled with lowered plasma lipoprotein levels and apparent stimulated glycolysis. Probiotic treatments also altered a diverse range of pathways outcomes, including amino-acid metabolism, methylamines and SCFAs. The novel application of hierarchical-principal component analysis allowed visualization of multicompartmental transgenomic metabolic interactions that could also be resolved at the compartment and pathway level. These integrated system investigations demonstrate the potential of metabolic profiling as a top-down systems biology driver for investigating the mechanistic basis of probiotic action and the therapeutic surveillance of the gut microbial activity related to dietary supplementation of probiotics.

Published
2008

27. Butyrate stimulates cyclin D and p21 and inhibits cyclin-dependent kinase 2 expression in HT-29 colonic epithelial cells

Author

Siavoshian, S., Hervé BLOTTIERE, Cherbut, C., Galmiche, Jp, Laboratoire de technologie appliquée à la nutrition, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1997

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