Search

Your search keyword '"Chepied, A."' showing total 22 results
Start Over Author "Chepied, A."
22 results on '"Chepied, A."'

1. An update on minding the gap in cancer

Author

Mesnil, Marc, Aasen, Trond, Boucher, Jonathan, Chépied, Amandine, Cronier, Laurent, Defamie, Norah, Kameritsch, Petra, Laird, Dale W., Lampe, Paul D., Lathia, Justin D., Leithe, Edward, Mehta, Parmender P., Monvoisin, Arnaud, Pogoda, Kristin, Sin, Wun-Chey, Tabernero, Arantxa, Yamasaki, Hiroshi, Yeh, Elizabeth S., Dagli, Maria Lucia Zaidan, and Naus, Christian C.

Published
2018
Full Text
View/download PDF

4. Involvement of the Gap Junction Protein, Connexin43, in the Formation and Function of Invadopodia in the Human U251 Glioblastoma Cell Line

Author

Amandine Chepied, Zeinaba Daoud-Omar, Annie-Claire Meunier-Balandre, Dale W. Laird, Marc Mesnil, and Norah Defamie

Subjects
cell invasion, connexin, gap junction, glioblastoma, invadopodia, Cytology, QH573-671
Abstract

The resistance of glioblastomas to treatments is mainly the consequence of their invasive capacities. Therefore, in order to better treat these tumors, it is important to understand the molecular mechanisms which are responsible for this behavior. Previous work suggested that gap junction proteins, the connexins, facilitate the aggressive nature of glioma cells. Here, we show that one of them—connexin43 (Cx43)—is implicated in the formation and function of invadopodia responsible for invasion capacity of U251 human glioblastoma cells. Immunofluorescent approaches—combined with confocal analyses—revealed that Cx43 was detected in all the formation stages of invadopodia exhibiting proteolytic activity. Clearly, Cx43 appeared to be localized in invadopodia at low cell density and less associated with the establishment of gap junctions. Accordingly, lower extracellular matrix degradation correlated with less mature invadopodia and MMP2 activity when Cx43 expression was decreased by shRNA strategies. Moreover, the kinetics of invadopodia formation could be dependent on Cx43 dynamic interactions with partners including Src and cortactin. Interestingly, it also appeared that invadopodia formation and MMP2 activity are dependent on Cx43 hemichannel activity. In conclusion, these results reveal that Cx43 might be involved in the formation and function of the invadopodia of U251 glioblastoma cells.

Published
2020
Full Text
View/download PDF

5. Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts

Author

Laurent Picot, Thierry Patrice, Jean-Paul Cadoret, Jean-Marie Piot, Mathieu Lafferriere, Isabelle Lanneluc, Valerie Thiery, Raymond Kaas, Jean-Baptiste Berard, Benoit Serive, Lucie Aumailley, Said Ihammouine, Amandine Chepied, Virginie Pasquet, and Perrine Morisset

Subjects
pigments, microalgae, Dunaliella, violaxanthin, carotenoid, cancer, apoptosis, Biology (General), QH301-705.5
Abstract

Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 µg·mL−1 (0.17 µM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 µg·mL−1 (13.3 µM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 µg·mL−1 (66.7 µM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.

Published
2011
Full Text
View/download PDF

6. Involvement of the Gap Junction Protein, Connexin43, in the Formation and Function of Invadopodia in the Human U251 Glioblastoma Cell Line

Author

Marc Mesnil, Annie-Claire Meunier-Balandre, Zeinaba Daoud-Omar, Amandine Chepied, Dale W. Laird, Norah Defamie, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Schulich School of Medicine and Dentistry, University of Western Ontario (UWO), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, connexin, MMP2, Connexin, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Models, Biological, Article, Small hairpin RNA, gap junction, 03 medical and health sciences, Cell Line, Tumor, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, lcsh:QH301-705.5, ComputingMilieux_MISCELLANEOUS, invadopodia, 030102 biochemistry & molecular biology, biology, Chemistry, Gap junction, glioblastoma, General Medicine, cell invasion, 3. Good health, Cell biology, 030104 developmental biology, lcsh:Biology (General), Connexin 43, Gene Knockdown Techniques, Invadopodia, Podosomes, Proteolysis, biology.protein, cardiovascular system, Gelatin, sense organs, biological phenomena, cell phenomena, and immunity, Extracellular Matrix Degradation, Cortactin, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Proto-oncogene tyrosine-protein kinase Src, Protein Binding
Abstract

The resistance of glioblastomas to treatments is mainly the consequence of their invasive capacities. Therefore, in order to better treat these tumors, it is important to understand the molecular mechanisms which are responsible for this behavior. Previous work suggested that gap junction proteins, the connexins, facilitate the aggressive nature of glioma cells. Here, we show that one of them&mdash, connexin43 (Cx43)&mdash, is implicated in the formation and function of invadopodia responsible for invasion capacity of U251 human glioblastoma cells. Immunofluorescent approaches&mdash, combined with confocal analyses&mdash, revealed that Cx43 was detected in all the formation stages of invadopodia exhibiting proteolytic activity. Clearly, Cx43 appeared to be localized in invadopodia at low cell density and less associated with the establishment of gap junctions. Accordingly, lower extracellular matrix degradation correlated with less mature invadopodia and MMP2 activity when Cx43 expression was decreased by shRNA strategies. Moreover, the kinetics of invadopodia formation could be dependent on Cx43 dynamic interactions with partners including Src and cortactin. Interestingly, it also appeared that invadopodia formation and MMP2 activity are dependent on Cx43 hemichannel activity. In conclusion, these results reveal that Cx43 might be involved in the formation and function of the invadopodia of U251 glioblastoma cells.

Published
2019
Full Text
View/download PDF

8. Connexin43 increases the invasion capacity of human glioblastoma cells by inducing the formation of invadopodia

Author

Chepied, Amandine, Balandre, Annie-Claire, Monvoisin, Arnaud, Cronier, Laurent, Mesnil, Marc, Defamie, Norah, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), chepied, amandine, and Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2017

9. An update on minding the gap in cancer

Author

Maria Lúcia Zaidan Dagli, Arnaud Monvoisin, Justin D. Lathia, Amandine Chepied, Christian C. Naus, Jonathan Boucher, Parmender P. Mehta, Petra Kameritsch, Wun Chey Sin, Paul D. Lampe, Laurent Cronier, Hiroshi Yamasaki, Trond Aasen, Elizabeth S. Yeh, Kristin Pogoda, Dale W. Laird, Marc Mesnil, Norah Defamie, Arantxa Tabernero, Edward Leithe, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Vall d’Hebron Research Institute (VHIR), Institut de physiologie et biologie cellulaires (IPBC), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Department of Anatomy and Cell Biology, University of Western Ontario (UWO), Centre for Cancer Biomedicine [Oslo] (CCB), Faculty of Medicine [Oslo], University of Oslo (UiO)-University of Oslo (UiO), Department of Cellular and Physiological Sciences, University of British Columbia (UBC), Unit of Multistage Carcinogenesis, International Agency for Cancer Research (IACR), Laboratory of Experimental Oncology, University of São Paulo (USP), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, History, [SDV]Life Sciences [q-bio], Biophysics, Gap Junctions, Library science, Cell Biology, Biochemistry, Neoplasm Proteins, 3. Good health, 03 medical and health sciences, Cell and Developmental Biology, 030104 developmental biology, 0302 clinical medicine, Neoplasms, 030220 oncology & carcinogenesis, Animals, Humans, CONEXINAS, Anatomy, Cancer Gap junction, Connexin, Tumor progression, Cell migration, Invasion, ComputingMilieux_MISCELLANEOUS
Abstract

This article is a report of the "International Colloquium on Gap junctions: 50 Years of Impact on Cancer" that was held 8-9 September 2016, at the Amphitheater "Pole Biologie Sante" of the University of Poitiers (Poitiers, France). The colloquium was organized by M Mesnil (Universite de Poitiers, Poitiers, France) and C Naus (University of British Columbia, Vancouver, Canada) to celebrate the 50th anniversary of the seminal work published in 1966 by Loewenstein and Kanno [Intercellular communication and the control of tissue growth: lack of communication between cancer cells, Nature, 116 (1966) 1248-1249] which initiated studies on the involvement of gap junctions in carcinogenesis. During the colloquium, 15 participants presented reviews or research updates in the field which are summarized below.

Published
2018
Full Text
View/download PDF

10. Connexin43 and glioblastoma invasion

Author

Chepied, Amandine, Defamie, Norah, chepied, amandine, Signalisation et Transports Ioniques Membranaires (STIM), Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

11. Perinatal exposure to dichloro-bisphenol A alters lipid composition of mouse liver

Author

El Hamrani, Dounia, Chepied, Amandine, Defamie, Norah, Même, William, Mesnil, Marc, Même, S., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and chepied, amandine

Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

12. Perinatal exposure to dichloro-bisphenol A alters hepatic lipid composition in mouse: a study by MRI and 1H MRS

Author

El Hamrani, Dounia, Chepied, Amandine, Même, William, Mesnil, Marc, Defamie, Norah, MÊme, S., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), chepied, amandine, and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

13. Connexin43 amplifies cerebral metabolits alteration due to Bisphenol A exposition

Author

Chepied, Amandine, El Hamrani, Dounia, turi, laurence, Mesnil, Marc, MÊme, S., Defamie, Norah, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), chepied, amandine, and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2015

14. Étude in vivo de l'exposition à de faibles doses de Bisphénol A. Implication de la Cx43

Author

Chepied, Amandine, El Hamrani, Dounia, turi, laurence, Mesnil, Marc, MÊme, S., Defamie, Norah, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), chepied, amandine, and Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2015

15. Une exposition périnatale aux dérivés dichlorés du Bisphénol A altère le métabolisme et la microarchitecture hippocampique : étude par IRM et 1H SRM chez la souris

Author

Hamrani, D. El, Chepied, A., Même, W., MESNIL, M., Defamie, N., MÊme, S., Frapart, Isabelle, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), and Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

16. Role of gap junctional intercellular communication in lymphangiogenesis and in prostate cancer cell adhesion to lymphatic endothelium

Author

Monvoisin, Arnaud, Simmoneau, C., Clarhaut, Jonathan, minaoui, a, Defamie, Norah, Chepied, Amandine, Mesnil, Marc, Cronier, Laurent, chepied, amandine, Signalisation et Transports Ioniques Membranaires (STIM), Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Inserm CIC 0802, Centre hospitalier universitaire de Poitiers (CHU Poitiers), and Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

17. Perinatal Bisphenol A exposure induces sex-specific modifications in lipid profil of mice liver

Author

El Hamrani, D., Chepied, A., Defamie, N., Même, W., MESNIL, M., Même, S., Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN), Université de Poitiers-Université de Tours-Centre National de la Recherche Scientifique (CNRS), and Frapart, Isabelle

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

18. Connexin43 mediates the invasive properties of human glioblastoma cells

Author

Chepied, Amandine, Balandre, Annie-Claire, Monvoisin, Arnaud, Cronier, Laurent, Mesnil, Marc, Defamie, Norah, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Poitiers, Institut de physiologie et biologie cellulaires (IPBC), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), and chepied, amandine

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2013

19. Etude de l’implication de la Connexine 43 dans le processus d’invasion des glioblastomes humains

Author

Chepied, Amandine, Monvoisin, Arnaud, Cronier, Laurent, Mesnil, Marc, Defamie, Norah, Signalisation et Transports Ioniques Membranaires (STIM), Université de Poitiers-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Tours-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), and chepied, amandine

Subjects
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.CAN] Life Sciences [q-bio]/Cancer, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, ComputingMilieux_MISCELLANEOUS, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract

International audience

Published
2013

20. Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts

Author

Amandine Chepied, Raymond Kaas, Lucie Aumailley, Laurent Picot, Mathieu Lafferriere, Jean-Marie Piot, Jean-Baptiste Bérard, Isabelle Lanneluc, Virginie Pasquet, Jean-Paul Cadoret, Valérie Thiéry, Benoît Serive, Said Ihammouine, Thierry Patrice, Perrine Morisset, LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Physiologie et biotechnologie des Algues (PBA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre hospitalier universitaire de Nantes (CHU Nantes), Mer, molécules et santé EA 2160 (MMS), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, and Université de Nantes (UN)-Université de Nantes (UN)

Subjects
0106 biological sciences, Dunaliella, pigments, Pharmaceutical Science, [SDV.CAN]Life Sciences [q-bio]/Cancer, Antineoplastic Agents, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, DNA Fragmentation, Xanthophylls, Biology, 01 natural sciences, Article, 03 medical and health sciences, chemistry.chemical_compound, microalgae, violaxanthin, carotenoid, cancer, apoptosis, Cell Line, Tumor, 010608 biotechnology, Drug Discovery, LNCaP, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:QH301-705.5, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), ComputingMilieux_MISCELLANEOUS, Chromatography, High Pressure Liquid, Cell Proliferation, 030304 developmental biology, 0303 health sciences, Cell growth, biology.organism_classification, 3. Good health, lcsh:Biology (General), chemistry, Biochemistry, Apoptosis, Cancer cell, DNA fragmentation, Growth inhibition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Violaxanthin
Abstract

International audience; Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose-dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 µg · mL −1 (0.17 µM). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 µg· mL −1 (13.3 µM) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 820 40 µg· mL −1 (66.7 µM). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.

Published
2011
Full Text
View/download PDF

21. Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts

Author

Pasquet, Virginie, Morisset, Perrine, Ihammouine, Said, Chepied, Amandine, Aumailley, Lucie, Berard, Jean-baptiste, Serive, Benoit, Kaas, Raymond, Lanneluc, Isabelle, Thiery, Valerie, Lafferriere, Mathieu, Piot, Jean-marie, Patrice, Thierry, Cadoret, Jean-paul, Picot, Laurent, Pasquet, Virginie, Morisset, Perrine, Ihammouine, Said, Chepied, Amandine, Aumailley, Lucie, Berard, Jean-baptiste, Serive, Benoit, Kaas, Raymond, Lanneluc, Isabelle, Thiery, Valerie, Lafferriere, Mathieu, Piot, Jean-marie, Patrice, Thierry, Cadoret, Jean-paul, and Picot, Laurent

Abstract

Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at concentrations as low as 0.1 mu g.mL(-1) (0.17 mu M). Phosphatidylserine exposure, typical of early apoptosis, was observed after 48 h treatment at 8 mu g.mL(-1) (13.3 mu M) but no DNA fragmentation, characteristic of late apoptosis steps, could be detected even after 72 h treatment at 40 mu g.mL(-1) (66.7 mu M). Taken together, our results demonstrate the strong antiproliferative activity of violaxanthin on one human mammary cancer cell line, and suggest that studying the pharmacology of violaxanthin and pharmacomodulated derivatives on cancer cells may allow potent antiproliferative drugs to be obtained.

Published
2011
Full Text
View/download PDF

22. Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts

Author

Pasquet, Virginie, primary, Morisset, Perrine, additional, Ihammouine, Said, additional, Chepied, Amandine, additional, Aumailley, Lucie, additional, Berard, Jean-Baptiste, additional, Serive, Benoit, additional, Kaas, Raymond, additional, Lanneluc, Isabelle, additional, Thiery, Valerie, additional, Lafferriere, Mathieu, additional, Piot, Jean-Marie, additional, Patrice, Thierry, additional, Cadoret, Jean-Paul, additional, and Picot, Laurent, additional

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results