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Propafenone (PPF) is a class I

X chromosome change has been proved to be associated with carcinogenesis and related to gender differences in cancer risk. If aberrant methylation of genes encoded by X chromosome involve in the risk and prognosis of cancers, including colorectal cancer (CRC), remain unclear. We conducted a case–control study consisted of 432 CRC cases and 434 controls, detecting the methylation levels of FAM156B, PIH1D3, and PPP1R3F in the X chromosome in blood leukocytes using methylation‐sensitive high‐resolution melting (MS‐HRM). We analyzed the relationship between the methylation levels and CRC susceptibility and then explored the interactions with environmental factors on CRC risk with logistics regression. Moreover, we conducted a follow‐up study containing 225 CRC patients to explore the associations between the methylation of FAM156B, PPP1R3F, and PIH1D3 and CRC prognosis. The hypermethylation of FAM156B, PPP1R3F, and PIH1D3 was related to increased CRC risk (ORPS‐adj = 2.932, 95% confidence interval [CI]: 2.029–4.237; ORPS‐adj = 1.602, 95% CI: 1.078–2.382; ORPS‐adj = 1.628, 95% CI: 1.065–2.490, respectively). In the multiple CpG site methylation (MCSM) analysis, compared with non‐MCSM, a significant relationship between MCSM and increased CRC risk was found (ORPS‐adj = 2.202, 95% CI: 1.512–3.208). We observed synergistic interaction between PPP1R3F hypermethylation and fried food consumption on CRC risk (ORi = 2.682, 95% CI: 1.321–5.446). However, there were no associations between the methylation of FAM156B, PPP1R3F, and PIH1D3 and CRC prognosis (p > 0.05). In conclusion, the methylation of FAM156B, PPP1R3F, and PIH1D3 genes in blood leukocytes is significantly related to CRC risk and may be potential biomarkers for CRC risk but not prognosis., Our results showed that the methylation of FAM156B, PPP1R3F, and PIH1D3 genes in blood leukocytes is significantly associated with colorectal cancer (CRC) risk and may be potential biomarkers for CRC risk.

Abnormal DNA methylation is considered a vital hallmark to regulate gene expression and influence the development and progression of colorectal cancer (CRC). Although CRC-related methylation prognostic models have been developed, their clinical application is limited due to the lack of external validation and extension to other survival evaluation indicators. Therefore, this study aimed to develop and validate novel methylation prognostic models correlated with different survival indicators for individualized prognosis prediction for CRC patients. The prognostic-related CpG sites of methylation-driven genes screened by the MethylMix algorithm were identified and validated in The Cancer Genome Atlas (TCGA) CRC methylation data and our methylation data. The prognostic models correlated with different survival evaluation indicators (overall survival [OS] and disease-free survival [DFS]) were developed and validated in the TCGA CRC dataset (N = 376) and our independent CRC dataset (N = 227). We utilized the combination of selected 3-CpG methylation sites in three genes (DAPP1, FAM3D, and PIGR) to construct a prognostic risk-score model. In the training dataset, Kaplan-Meier survival analysis demonstrated that high-risk patients had significantly poorer survival than low-risk patients (pOS = .0014; pDFS

Early and specific detection of cancer provides an opportunity for appropriate treatment. Although studies have suggested that

Human papillomavirus type 124 (HPV124), a member of betapapillomavirus, is associated with viral warts, actinic keratosis, and squamous cell carcinoma incidence in human. No reports of HPV124 infection in mainland China have been reported to date. In a survey of Chinese horses, we detected HPV124 via next-generation sequencing (NGS) in nasal swabs from 42% (39/93) of Thoroughbred horses, 7% (2/30) of the Akhal-Teke horses, and 17/50 aborted fetal lung tissues of Yili mares that were initially found to be negative for equid herpesvirus 1, which indicated the virus can be trans-species infection in different breeding horses, and suggested that this pathogen may potentially contribute to abortion incidence among horses. Further investigation showed that some workers involved in horse farm were positive for HPV124, which strongly suggested that horse-origin HPV124 strain may be transmissible from human workers to horses. Sequence analyses of E6 gene sequences from 18 Chinese HPV124 samples were performed, revealing that the 7 of 18 HPV124 strain identified both horses and human in the present study shared 100% sequence identity with previously published HPV124 isolate. Phylogenetic analyses based on E6 gene sequences in GenBank further revealed that the HPV124 isolate identified herein was closely related to the NJ3900 HPV124 reference strain, clustering together with the established Beta 1 HPV lineage. These results provide first evidence that HPV124 can infect horses, may function as a causative agent for abortions in Yili horses, and come from HPV124-positive humans laboring.

Human papillomavirus type 124 (HPV124) is a betapapillomavirus that is associated with viral warts, actinic keratosis, and squamous cell carcinoma incidence. No reports of HPV124 infection in mainland China have been reported to date. In a survey of Chinese horses, we detected HPV124 via next-generation sequencing (NGS) in nasal swabs from 4% (2/56) of thoroughbred horses, and 7% (2/30) of the Akhal-Teke horses, suggesting that horses can serve as a reservoir species for this virus. In addition, HPV124 was detected in 17/50 lung tissue samples from aborted Yili horse fetuses that were initially found to be negative for equid herpesviruses and equine arteritis virus, suggesting that this pathogen may potentially contribute to abortion incidence among horses. Further investigation showed that all workers involved in Yili mare production of farm B were positive for HPV124, while all other assessed individuals in farm B or without any history of contact with horses were negative for this virus. indicating that this HPV124 strain may be transmissible from horses to human workers at an HPV124-positive farm. Sequence analyses of E6 gene sequences from 18 Chinese HPV124 samples were performed, revealing that the 7 of 18 HPV124 strain identified among horses in the present study shared 100% sequence identity with previously published HPV124 isolate. Phylogenetic analyses based on E6 gene sequences in GenBank further revealed that the HPV124 isolate identified herein was closely related to the NJ3900 HPV124 reference strain, clustering together with the established Beta 1 HPV lineage. These results provide new evidence that HPV124 can infect Chinese horses, may function as a causative agent for abortions in Yili horses, and may also infect humans laboring in Yili mare stables.

Background: Nine different species of Equus caballus papillomavirus (EcPV) and three bovine papillomavirus (BPV) have been reported to infect horses, however, there are so far no describing such infections in China. In January 2021, an abortion storm occurred in Yili horses, as a result of which 50 out of 93 aborted fetus samples were found to be negative for equid herpesvirus (EHV) and equine arteritis virus (EAV).Results: In our pioneer study with Chinese horses, we first found EcPV-2 in the nasal swabs (4/230, 1.7%) of Yili horses, and semen (3/18, 16.7%) of the Thoroughbred horses. This indicated that EcPV can be indeed hosted by horses in China, and that EcPV-2 might be transmitted though breeding. Further detection of EcPVs in the lung tissues of aborted fetus in Yili horses, which were originally negative for equid herpesviruses, established that EcPV-2 was positive in 19 of 50 samples, thereby indicating that EcPV-2 might be a new pathogen causing of abortions. Thereafter, the sequence analyses for L1 genes sequences of 26 China’s EcPV-2 were performed which indicated that EcPV-2, that primarily infected the horses in China, shared 98.3%-99.9% nt identity with the already published sequences for EcPV-2. These observations indicated that EcPV-2 identified in the current study were highly similar variants of the previously identified strains of EcPV-2. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-2, found in the Chinese horses, was closely related to and clustered together with an already known EcPV-2a lineage. Conclusion: Our study provides the first evidence related to EcPV-2 infection in the Chinese horses, which can serve as a causative agent for Yili horse abortions, and thus can possibly lay the foundation for a systematic and detailed epidemiological study of this infection in the Chinese horses.

Chemodynamic therapy (CDT), a novel cancer therapeutic strategy defined as the treatment using Fenton or Fenton-like reaction to produce •OH in the tumor region, was first proposed by Bu, Shi, and co-workers in 2016. Recently, with the rapid development of Fenton and Fenton-like nanomaterials, CDT has attracted tremendous attention because of its unique advantages: 1) It is tumor-selective with low side effects; 2) the CDT process does not depend on external field stimulation; 3) it can modulate the hypoxic and immunosuppressive tumor microenvironment; 4) the treatment cost of CDT is low. In addition to the Fe-involved CDT strategies, the Fenton-like reaction-mediated CDT strategies have also been proposed, which are based on many other metal elements including copper, manganese, cobalt, titanium, vanadium, palladium, silver, molybdenum, ruthenium, tungsten, cerium, and zinc. Moreover, CDT has been combined with other therapies like chemotherapy, radiotherapy, phototherapy, sonodynamic therapy, and immunotherapy for achieving enhanced anticancer effects. Besides, there have also been studies that extend the application of CDT to the antibacterial field. This review introduces the latest advancements in the nanomaterials-involved CDT from 2018 to the present and proposes the current limitations as well as future research directions in the related field.

In this paper, CoNi bimetallic nanoparticle decorated reduced graphene oxide (CoNi-RGO) was synthesized by a facile hydrothermal method. When applied this CoNi-RGO into counter electrode for dye-sensitized solar cells (DSSCs), it shows smaller charge-transfer resistance and better electrocatalytic activity than that of pure reduced graphene oxide (RGO). At the optimized conditions, the energy conversion efficiency of DSSCs based on CoNi-RGO counter electrode was 3.79%, indicating a higher photovoltaic performance of DSSCs based on CoNi-RGO counter electrode than that of DSSCs based on RGO counter electrode (1.37%), and comparable to the value of DSSCs based on Pt counter electrode as a reference (4.95%). Additionally, the photovoltaic performance of DSSCs based on CoNi-RGO counter electrode strongly depends on its composition. The molar ratio of Co/Ni and the weight ratio of CoNi/GO that used to prepare CoNi-RGO counter electrode are key factors to affect the performance of their cell devices. When both the molar ratio of Co/Ni and the weight ratio of CoNi/GO are 1:1, the CoNi-RGO counter electrode shows the best performance, indicating the potential of such bimetallic nanoparticle decorated RGO as low-cost and efficient counter electrode to replace noble metal Pt counter electrode for the practical application of DSSCs devices.

Nanoparticles of the bimetallic alloy CoNi wrapped in nitrogen-doped graphite-like carbon shells and dispersed on nitrogen-doped graphite-like carbon sheets (CoxNi1−x@NC) were synthesized by calcining CoNi metal–organic frameworks that were prepared through a facile solvothermal reaction using various raw-material molar ratios Co:Ni and CoNi:ethylenedinitrilotetraacetic acid. After depositing CoxNi1−x@NC for use as a counter electrode film in dye-sensitized solar cells, it was found that the electrocatalytic activity of the CoxNi1−x@NC counter electrode towards triiodide reduction could be optimized by simply tuning the molar ratios (Co:Ni and CoNi:ethylenedinitrilotetraacetic acid) appropriately during CoxNi1−x@NC synthesis. Cells that utilized a CoxNi1−x@NC counter electrode exhibited strong chemical-composition-dependent photovoltaic performance. Under optimal conditions, the CoxNi1−x@NC counter electrode presented an impressive energy conversion efficiency of 3.58%, suggesting that it is a highly promising counter electrode for application in dye-sensitized solar cells. This counter electrode has the advantages that it is considerably less expensive than a Pt counter electrode and that it provides the basis for the design and preparation of other inexpensive and efficient counter electrodes to replace Pt.

In terahertz (THz) wave parametric sources, the large angle between the pump and terahertz waves (about 65° in LiNbO3 crystal) will affect the spatial intensity distributions. To investigate how the noncollinear phase-matching affects the spatial intensity properties of the pump, Stokes, and THz waves, we take the injection-seeded THz-wave parametric generator (is-TPG) as an example to study the beam spatial intensity properties via the numerical solutions of the coupled-wave equations. The simulated results demonstrate that the pump and Stokes intensities have significant changes along the THz-wave propagation direction. It is the first time that the spatial intensity distributions of the three waves in the THz-wave parametric sources have been studied in theory. In addition, the output intensity distributions of the pump, Stokes, and THz waves are measured in the designed is-TPG. The measured spatial intensity distributions are in coincidence with the simulated results. Investigations on the beam spatial intensity properties provide a potential approach to improve the THz-wave outputs and the pump conversion efficiency for the is-TPG and other THz-wave parametric sources.

Two deep learning models to reconstruct three-dimensional (3D) steady-state rotating flows are proposed to capture the spatial information: the 3D convolutional encoder–decoder and the 3D convolutional long short-term memory model. They are based on deep learning methods such as the encoder–decoder convolutional neural network and recurrent neural network. Their common components are an encoder, a middle layer, and a decoder. The rotating flows in a stirred tank with four inclined blades are calculated for the dataset to train and test the two models. A workflow for the flow field reconstruction is established and all variants made up of various components are executed according to the flow. The optimal networks of the two models are selected by comparing performance measures. The results show that both models have the excellent ability to fit the 3D rotating flow field. Performance measures of the second model are better than those of the first one, but its running time is slower than that of the first one. In practice, this method can be used in the design and optimization of stirred tanks, centrifugal pumps, and other machines with rotating parts.

BackgroundAberrant DNA methylation is a critical regulator of gene expression and plays a crucial role in the occurrence, progression, and prognosis of colorectal cancer (CRC). We aimed to identify methylation-driven genes by integrative epigenetic and transcriptomic analysis to predict the prognosis of CRC patients.MethodsMethylation-driven genes were selected for CRC using a MethylMix algorithm and LASSO regression screening strategy, and were further used to construct a prognostic risk-assessment model. The Cancer Genome Atlas (TCGA) database was obtained as the training set for both the screening of methylation-driven genes and the effect of genes signature on CRC prognosis. Then, the prognostic genes signature was validated in three independent expression arrays of CRC data from Gene Expression Omnibus (GEO).ResultsWe identified 143 methylation-driven genes, of which the combination of BATF, PHYHIPL, RBP1, and PNPLA4 expression levels was screened as a better prognostic model with the best area under the curve (AUC) (AUC = 0.876). Compared with patients in the low-risk group, CRC patients in the high-risk group had significantly poorer overall survival in the training set (HR = 2.184, 95% CI: 1.404–3.396, P < 0.001). Similar results were observed in the validation set. Moreover, VanderWeele’s mediation analysis indicated that the effect of methylation on prognosis was mediated by the levels of their expression (HRindirect = 1.473, P = 0.001, Proportion mediated, 69.10%).ConclusionsWe identified a four-gene prognostic signature by integrative analysis and developed a risk-assessment model that is significantly associated with patients’ survival. Methylation-driven genes might be a potential prognostic signature for CRC patients.

Background: Nine species of Equus caballus papillomavirus (EcPV) have been reported to infect horses, however, there are so far no reports of such infections in China. Results: In our pioneer study with Chinese horses, we found EcPV-1 in intranasal papilloma and nasal swabs, EcPV-2 in nasal swabs and semen, and EcPV-7 primarily in semen. This indicates that EcPVs are indeed hosted by horses in China, and that EcPV-2 and 7 may be getting transmitted though breeding. Sequence analyses for complete genomic sequences of EcPV-1 (G2), EcPV-2 (XJ-KS1391) and EcPV-7 (XJ-zs1) were performed which indicated that EcPV-1, 2 and 7, that infect horses in China, share 99.3% nt identity with the already published sequences for EcPV-1, 2 and 7. These observations indicate that three types of EcPVs identified in the current study are highly similar variants of previously known types of EcPV-1, 2 and 7. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-1, 2 and 7, found in Chinese horses, are closely related to and clustered together with already known EcPV-1, 2 and 7, respectively. Conclusion: Our study provides a novel evidence for EcPVs infection and circulation in Chinese horses and thus lays the foundation for a systematic and detailed epidemiological study of these infections in Chinese horses.

Methylation alterations of imprinted genes lead to loss of imprinting (LOI). Although studies have explored the mechanism of LOI in breast cancer (BC) development, the association between imprinted gene methylation in peripheral blood and BC risk is largely unknown. We utilized HumanMethylation450 data from TCGA and GEO (n = 1461) to identify the CpG sites of imprinted genes associated with BC risk. Furthermore, we conducted an independent case-control study (n = 1048) to validate DNA methylation of these CpG sites in peripheral blood and BC susceptibility. cg26709929, cg08446215, cg25306939, and cg16057921, which are located at KCNQ1, KCNQ1OT1, and PHLDA2, were discovered to be associated with BC risk. Subsequently, the association between cg26709929, cg26057921, and cg25306939 methylation and BC risk was validated in our inhouse dataset. All 22 CpG sites in the KCNQ1OT1 region were associated with BC risk. Individuals with a hypermethylated KCNQ1OT1 region (>0.474) had a lower BC risk (OR: 0.553, 95% CI: 0.397−0.769). Additionally, the methylation of the KCNQ1OT1 region was not significantly different among B cells, monocytes, and T cells, which was also observed at CpG sites in PHLDA2. In summary, the methylation of KCNQ1, KCNQ1OT1, and PHLDA2 was associated with BC risk, and KCNQ1OT1 methylation could be a potential biomarker for BC risk assessment.

In November 2018, an outbreak of respiratory disease occurred in foals at an equestrian club in Changji, northern Xinjiang, China. We applied viral metagenomics to investigate this outbreak and identify potential pathogens involved in this equine respiratory syndrome. The metagenomics data revealed the presence of sequences matching those of equid herpesvirus (EHV) 2, 4, and 5. PCR with specific primers targeting ORF33 of EHV-4 and ORF8 of EHV-2 and EHV-5 revealed coinfection with these viruses in this respiratory syndrome. To investigate the prevalence of these viruses in China, 453 nasal swabs from clinically healthy thoroughbred foals (36/453, 7.9%) and horses (417/453, 92.1%) were collected from several equestrian clubs. Forty-five (9.9%) of the samples tested positive for EHV-5 DNA, and seven (1.5%) tested positive for EHV-2, but all were negative for EHV-4 DNA. Forty-nine (10.8%) samples tested positive for both EHV-5 and EHV-2 DNA. Using these samples, one complete EHV-4 ORF33, 10 partial EHV-2 ORF8, and 50 partial EHV-5 ORF8 sequences from the 10 diseased foals and 50 thoroughbred horses were then determined. Sequence analysis indicated that EHV-4 ORF33 and EHV-5 ORF8, in contrast to EHV-2 ORF8, had high sequence similarity to those of published sequences. Our data provide the first evidence that EHV-2, -4, and -5 co-circulate in China and that EHV-4 is potentially involved in this respiratory disease in foals.

An increasing number of Internet applications are applying microservice architecture due to its flexibility and clear logic. The stability of microservice is thus vitally important for these applications' quality of service. Accurate failure root cause localization can help operators quickly recover microservice failures and mitigate loss. Although cross-microservice failure root cause localization has been well studied, how to localize failure root causes in a microservice so as to quickly mitigate this microservice has not yet been studied. In this work, we propose a framework, MicroCause, to accurately localize the root cause monitoring indicators in a microservice. MicroCause combines a simple yet effective path condition time series (PCTS) algorithm which accurately captures the sequential relationship of time series data, and a novel temporal cause oriented random walk (TCORW) method integrating the causal relationship, temporal order, and priority information of monitoring data. We evaluate MicroCause based on 86 real-world failure tickets collected from a top tier global online shopping service. Our experiments show that the top 5 accuracy (AC@5) of MicroCause for intra-microservice failure root cause localization is 98.7%, which is greatly higher (by 33.4 %) than the best baseline method.

A healthy body is the foundation of everything. The indoor environment comfort of fitness places has been paid more and more attention. However, there is a lack of relevant standards for the thermal environment of indoor sports and fitness venues in China, especially the research on the indoor thermal environment in different seasons. Therefore, in our study, physical environment parameters such as indoor temperature and humidity, black ball temperature, air flow rate and CO2 concentration in 9 indoor fitness places in Xi ‘an in different seasons were measured on site. At the same time, a subjective questionnaire survey was conducted on thermal sensation and acceptability of the people who were exercising in the fitness places. Through subjective questionnaire analysis, respondents generally have a high degree of satisfaction and acceptance of temperature, and the average thermal comfort vote is the highest when the indoor temperature is 17.9-23.9°C. In addition, the thermal comfort of different sports in the same space also has certain differences. The fitness people indoor have lower satisfaction with air flow and air quality. Based on the study of the factors affecting indoor thermal comfort, this paper puts forward the improvement strategies for improving indoor thermal comfort, and provides scientific basis for the design and operation of fitness venues.

Terahertz parametric oscillator based on RbTiOPO4 (RTP) crystal utilizing the surface-emitted configuration under high pumping pulse energy and low pulse repetition rate is investigated. The obtained THz wave was discontinuously tuned from 3.01 to 3.09 THz, from 3.53 to 4.17 THz, from 4.54 to 4.66 THz, and from 5.35 to 5.89 THz, respectively. The maximum output THz wave pulse energy was 1.77 μJ at 3.85 THz. It was obtained when the pumping pulse energy was 120 mJ and the pumping beam diameter was 3.0 mm. The polarization of THz wave was along the z axis of the RTP crystal. The THz beam on its output surface was approximately elliptical with a major axis of 6.5 mm and a minor axis of 3.5 mm. The divergence angles were 2.45° in the horizontal direction, and 3.88° in the vertical direction.

A 2-20 GHz limiter low noise amplifier (LNA) based on 0.15 µm GaAs pHEMT technology is designed and fabricated. The limiter and LNA are integrated on same substrate. To achieve ultra-wideband performance, LNA is designed by cascaded common gate-common source and distributed topology. On-wafer measured results show that during working frequency, the proposed limiter LNA has yielded gain of 18+1 dB, noise figure (NF) of 1.6-3.2 dB and 1 dB compression point is above 14 dBm. The total power consumption is 350mW@5V. The limiter LNA can withstand 20 dBm pulse power (2ms, 30% duty cycle). The total size of chip is 4.8 x 2 mm^2.

Piezo proteins are a group of important mechanical-responsive ion channel proteins, which play an indispensable role in the normal physiological process of the organism. Therefore, Piezo proteins have attracted many researchers to develop relevant studies. In this work, an evolutionary tree of the Piezo1 gene was constructed using the gene sequences from National Center for Biotechnology Information (NCBI). Using MEGA, the ancestral gene sequence was constructed and the conserved and variable regions of Piezo1 protein were marked. According to the tree, the evolution of Piezo genes can be analysed, and then it is possible to predict the direction of gene mutation. The pathogenic mutation site to the corresponding regions was also mapped. Although there is still much work to be improved, the efforts to analyze the Piezo 1 gene from an evolutionary perspective is valuable, which might be helpful for future disease diagnosis and individualized therapy.

Host inflammation is a critical component of tumor progression and its status can be indicated by peripheral blood cell counts. We aimed to construct a comprehensively prognostic inflammatory index (PII) based on preoperative peripheral blood cell counts and further evaluate its prognostic value for patients with colorectal cancer (CRC). A total of 9315 patients with stage II and III CRC from training and external validation cohorts were included. The PII was constructed by integrating all the peripheral blood cell counts associated with prognosis in the training cohort. Cox analyses were performed to evaluate the association between PII and overall survival (OS) and disease-free survival (DFS). In the training cohort, multivariate Cox analyses indicated that high OS-PII (&gt, 4.27) was significantly associated with worse OS (HR: 1.330, 95% CI: 1.189&ndash, 1.489, p &lt, 0.001), and high DFS-PII (&gt, 4.47) was significantly associated with worse DFS (HR: 1.366, 95% CI: 1.206&ndash, 1.548, p &lt, 0.001). The prognostic values of both OS-PII and DFS-PII were validated in the external validation cohort. The nomograms achieved good accuracy in predicting both OS and DFS. Time-dependent ROC analyses showed that both OS-PII and DFS-PII have a stable prognostic performance at various follow-up times. The prognostic value of tumor-node-metastasis staging could be enhanced by combining it with either OS-PII or DFS-PII. We demonstrated that PIIs are independent prognostic predictors for CRC patients, and the nomograms based on PIIs can be recommended for personalized survival prediction of patients with CRC.

The combination of a Stokes parametric oscillator and a Stokes parametric amplifier is used to generate high-energy terahertz pulses in KTiOPO4 (KTP) crystal at 5.70 THz. The Stokes parametric oscillator generates Stokes pulses first. And then they are amplified in the Stokes parametric amplifier and at the same time, high energy terahertz pulses are generated via stimulated polariton scattering. When the incident Stokes pulse energy is 36.6 mJ and the pump energy is 580.0 mJ, the amplified Stokes energy is 192.1 mJ and the obtained maximum terahertz pulse energy is 17.0 μJ. Compared with the reported terahertz pulse energy obtained by the KTiOPO4 terahertz parametric oscillators or generators, the terahertz pulse energy is greatly enhanced. In addition, considering the large angle non-collinear phase matching, pump beam depletion, terahertz wave surface-emitted structure, Gaussian pulse distribution, we make a theoretical simulation for the Stokes parametric amplifier through the coupled-wave equations. The theoretical results are in agreement with the experimental results on the whole.

In the presence of cholecystitis or portal hypertension, hemorrhage is common during laparoscopic cholecystectomy (LC) because the vessels of Calot’s triangle are fragile and tortuous. Bleeding can obstruct surgical field visibility and increase conversion rates and risk of common bile duct injury. The Pringle maneuver is a simple occlusion approach that could limit blood flow from the hepatic pedicle, thus controlling bleeding to provide a clear surgical field to reduce conversion rate. In this study, we aimed to investigate the feasibility, effectiveness and safety of hepatic pedicle occlusion with the Pringle maneuver during difficult LC. From 2011 to 2015, LC with hepatic pedicle occlusion by the Pringle maneuver was performed in 67 patients (Pringle group). Another group of 67 cases with matched clinical parameters where LC was performed without the Pringle maneuver (non-Pringle group) was retrieved from a database to serve as the control group. The Pringle group had a significantly lower conversion rate (1.49% vs. 11.9%; P = 0.038), less blood loss (37.5 ± 24.1 mL vs. 94.5 ± 67.8 mL; P = 0.002), shorter postoperative hospitalization (2.5 ± 1.4 days vs. 3.5 ± 2.5 days; P = 0.005), and lower cost ($1343 ± $751 USD vs. $1674 ± $609 USD; P = 0.024) than non-Pringle group. There was one case each of bile duct injury and readmission within 30 days because of bile leakage in the non-Pringle group, but none in the Pringle group. Hepatic pedicle occlusion could provide a clear surgical field and enable the recognition of structures during LC. The Pringle maneuver offers a feasible and safe approach to lower conversion rates in difficult LC.

Laparoscopic liver resection (LLR) has been the most impressive development in the field of liver surgery in recent two decades. Technical innovations and experience accumulation have made LLR a safe and effective procedure with faster postoperative recovery. Despite the fast spreading of the procedure, details regarding the indications, oncological outcomes and technical essentials were still disputable. To address these issues, two international consensus conferences were hold to update the knowledge in this field. The statements of the both conferences were not conclusive and more high-quality researches are required. In this article, we reviewed the development and the current state of LLR. Indications, outcomes, surgical techniques and devices used in LLR were also discussed.

By using the polariton dispersion relation in KTiP0 4 crystal and the coupled wave equations for stimulated polariton scattering, a semiquantitative explanation for the discontinuous tunability of the KTiP04 terahertz parametric oscillator is given.