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Through the method of three-dimensional numerical simulation, the rotating detonation combustion characteristics of kerosene-fueled scramjets in the range of Ma＝3~7 were studied. In the flight condition of Ma=3, due to the poor effect of fuel atomization and evaporation, the detonation combustion of kerosene fuel cannot be realized. In the flight conditions of Ma＝4, 5, 6, with the increase of Mach number, the number of wave heads increases gradually, which are single-wave, three-wave, and five-wave modes, respectively. However, the propagation speed gradually decreases. In the scramjet mode, the liquid fuel has a good effect of atomization and evaporation. Nevertheless, kerosene vapor remains in the flow field to varying degrees and is discharged from the combustion chamber without participating in the reaction. In the flight condition of Ma＝7, the flow field will burn in a stationary detonation mode because the incoming flow is close to CJ velocity.

This study conducted 3D numerical simulations to investigate the impact of combustion chamber diameter on the combustion characteristics of liquid kerosene scramjet rotating detonation under high Mach number flow conditions (Ma6/28 km). After ignition, a local hotspot near the contact surface between the combustion product and fresh reactant facilitates the generation of new detonation wave heads, resulting in a co-directional multi-wave mode in the detonation combustion flow field. The higher total temperature of the incoming flow restricts the accumulation of a substantial fuel gas layer in the axial direction of the combustion chamber, resulting in a smaller fuel distribution area and a lower wave head. Increasing the inner diameter of the combustion chamber leads to an increase in the number of wave heads but a decrease in overall height. Specifically, when using diameters of 125 and 150 mm, we observed significant periodic low-frequency oscillations in the peak pressure of the detonation wave during stable propagation. The specific impulse of the fuel does not vary significantly across different combustion chamber diameters. However, when the inner diameter is 75 mm, periodic oscillations occur, which reduce thrust stability. These findings provide valuable insights into optimizing combustion chamber design and improving the efficiency and stability of liquid kerosene scramjet rotating detonation propulsion systems under high Mach number flow conditions.

Two-phase rotating detonation ramjets are considered to be suitable for aerospace applications due to their high thermodynamic cycle efficiency. These engines have an extremely complex internal flow field, in which the liquid fuel undergoes physical and chemical processes such as fragmentation, evaporation, mixing, and combustion; these processes also interact with detonation waves that have significant gradients. This makes it difficult to simulate a three-dimensional (3D) full-process rotating detonation combustion chamber. Here, based on the Euler–Lagrangian simulation method, a 3D numerical combustion chamber was simulated using kinetic theory and the constant thermal physical property parameter (TPPP) calculation method. The accuracy of these methods was then compared with the existing experimental results and theoretical values. Calculating the TPPPs using kinetic theory brought about a relatively high-pressure peak and detonation wave temperature; the detonation wave profile was also finer and more precise. The detonation wave propagation velocity of the two-phase detonation is estimated to be about 60% of the theoretical gas-phase CJ velocity. The calculation method of physical parameters has relatively little influence on the engine’s operating frequency and the detonation wave's propagation velocity but has a more significant influence on the peak pressure. Constant TPPPs can be used when the Kelvin–Helmholtz–Rayleigh–Taylor model with insufficient precision is used to consider the breakup of droplets and leads to the acceleration of the propagation speed of two-phase detonation waves.

Vegetation restoration is assumed to enhance carbon (C) sequestration in terrestrial ecosystems, where plant producers and microbial decomposers play key roles in soil C cycling. However, it is not clear how the nutrient limitation patterns of plants and soil microbes might change during vegetation restoration. We investigated the nutrient limitations of the plant and microbial communities along a natural vegetation restoration chronosequence (1, 8, 16, 31, and 50 years) following farmland abandonment in Qinling Mountains, China, and assessed their relationships with soil factors. The result showed that following natural vegetation restoration, the nitrogen (N) limitation of plant and microbial communities was alleviated significantly, and thereafter, it began to shift to phosphorus (P) limitation at a later stage. Plants showed P limitation 50 years after restoration, while microbial P limitation appeared 31 years later. The changes in plant nutrient limitation were consistent with those in microbial nutrient limitation, but soil microbes were limited by P earlier than plants. Random forest model and partial least squares path modeling revealed that soil nutrient stoichiometry, especially soil C:N ratio, explained more variations in plant and microbial nutrient limitation. Our study demonstrates that the imbalanced soil C:N ratio may determine the soil microbial metabolic limitation and further mediate the variation in plant nutrient limitation during natural vegetation restoration, which provides important insights into the link between metabolic limitation for microbes and nutrient limitation for plants during vegetation restoration to improve our understanding of soil C turnover in temperate forest ecosystems.

Abstract Background Given the limited treatment options available for oral lichen planus (OLP), a study was undertaken to obtain preliminary information on the therapeutic efficacy of tinidazole mouth rinse in patients with OLP. Methods A prospective, open-label pilot study was conducted to assess the efficacy of thrice-daily tinidazole mouth rinse for one week in OLP patients (n = 27). Reticulation/erythema/ulceration (REU) scores and visual analog scale (VAS) scores were used to measure lesions at baseline and after one week of treatment. Mucosal samples were collected, and the abundance of Fusobacterium nucleatum was quantified using RT-PCR. Statistical analysis using t-test, Wilcoxon signed rank test and Pearson correlation test. Results After treatment, VAS scores significantly decreased in both reticular (P = 0.03) and erosive OLP patients (P = 0.003). However, REU scores significantly decreased only in erosive OLP patients (P = 0.002). The relative abundance of Fusobacterium nucleatum on the damaged mucosa surface significantly decreased in all OLP patients (P = 0.01). In erosive OLP patients, the triamcinolone group showed a significantly greater improvement in VAS scores compared to the tinidazole group (P = 0.01). However, there was no statistically significant correlation between the relative abundance of Fusobacterium nucleatum and REU scores in OLP patients (r = 0.0754, P = 0.61). Conclusion Tinidazole mouth rinse showed potential in reducing disease severity in OLP patients and was well-tolerated, suggesting its viability as a local therapeutic option. However, randomized controlled studies are warranted to confirm these preliminary findings.

Metallic components with surface microfeatures are increasingly used in aerospace, automotive and other applications due to their drag-reducing properties. In this situation, we proposed a novel shape-surface integrated incremental sheet forming (S2-ISF) process to achieve the simultaneous fabrication of both surface microfeatures and desired geometric shape. It was found that an increase in the forming angle was most effective in improving the formation of microgroove array, followed by the step size, the spindle speed and the feed rate. Moreover, when the forming angle is constant, the step size and microgroove pitch increase linearly. Compared to the as-received sheet, the yield strength is increased by more than 20% after the S2-ISF process. Moreover, we observed that the increase of forming angle in the S2-ISF process has promoted grain deformation and grain fragmentation, which is favorable for the formed part to obtain more uniform and finer grains. The grain size distribution along the thickness direction shows the characteristics of ‘coarse in the middle and fine at both ends’. Finally, we found that the formed parts with different microgroove pitch from the S2-ISF process have the significant enhancement on the hydrophobicity and anti-friction property. When microgroove pitch is 155.5 μm, the surface contact angle of formed part improved from 70.65° to 101.39°, and the wear volume of formed part decreases from 4.89 × 10−3 mm3 to 3.65 × 10−3 mm3. This work provides a solution for the efficient and economical fabrication of three-dimensional thin-walled parts with microgroove array to enhance surface functionality.

Abstract Coix lacryma-jobi L. is one of the most economically and medicinally important corns. This study constructed a high-density genetic linkage map of C. lacryma-jobi based on a cross between the parents 'Qianyi No. 2' × 'Wenyi No. 2' and their F2 progeny through high-throughput sequencing and the construction of a specific-locus amplified fragment (SLAF) library. After pre-processing, 325.49 GB of raw data containing 1628 M reads were obtained. A total of 22,944 high-quality SLAFs were identified, among which 3952 SLAFs and 3646 polymorphic markers met the requirements for the construction of a genetic linkage map. The integrated map contained 3605 high-quality SLAFs, which were grouped into ten genetic linkage groups. The total length of the map was 1620.39 cM, with an average distance of 0.45 cM and an average of 360.5 markers per linkage group. This report presents the first high-density genetic map of C. lacryma-jobi. This map was constructed using an F2 population and SLAF-seq approach, which allows the development of a large number of polymorphic markers in a short period. These results provide a platform for precise gene/quantitative trait locus (QTL) mapping, map-based gene separation, and molecular breeding in C. lacryma-jobi. They also help identify a target gene for tracking, splitting quantitative traits, and estimating the phenotypic effects of each QTL for QTL mapping. They are of great significance for improving the efficiency of discovering and utilizing excellent gene resources of C. lacryma-jobi.

Safflower (Carthamus tinctorius L.), a member of the Asteraceae family, is widely used in traditional herbal medicine. This review summarized agronomic conditions, genetic diversity, clinical application, and phytochemicals and pharmacological properties of safflower. The genetic diversity of the plant is rich. Abundant in secondary metabolites like flavonoids, phenols, alkaloids, polysaccharides, fatty acids, polyacetylene, and other bioactive components, the medicinal plant is effective for treating cardiovascular diseases, neurodegenerative diseases, and respiratory diseases. Especially, Hydroxysafflor yellow A (HYSA) has a variety of pharmacological effects. In terms of treatment and prevention of some space sickness in space travel, safflower could be a potential therapeutic agent. Further studies are still required to support the development of safflower in medicine. Our review indicates that safflower is an important medicinal plant and research prospects regarding safflower are very broad and worthy of further investigation.

Abstract G protein-coupled receptors (GPCRs) play a key role in regulating the homeostasis of the internal environment and are closely associated with tumour progression as major mediators of cellular signalling. As a diverse and multifunctional group of proteins, the G protein signalling regulator (RGS) family was proven to be involved in the cellular transduction of GPCRs. Growing evidence has revealed dysregulation of RGS proteins as a common phenomenon and highlighted the key roles of these proteins in human cancers. Furthermore, their differential expression may be a potential biomarker for tumour diagnosis, treatment and prognosis. Most importantly, there are few systematic reviews on the functional/mechanistic characteristics and clinical application of RGS family members at present. In this review, we focus on the G-protein signalling regulator (RGS) family, which includes more than 20 family members. We analysed the classification, basic structure, and major functions of the RGS family members. Moreover, we summarize the expression changes of each RGS family member in various human cancers and their important roles in regulating cancer cell proliferation, stem cell maintenance, tumorigenesis and cancer metastasis. On this basis, we outline the molecular signalling pathways in which some RGS family members are involved in tumour progression. Finally, their potential application in the precise diagnosis, prognosis and treatment of different types of cancers and the main possible problems for clinical application at present are discussed. Our review provides a comprehensive understanding of the role and potential mechanisms of RGS in regulating tumour progression. Video Abstract

Abstract Background Burning mouth syndrome (BMS) is a complex chronic pain disorder that significantly impairs patients' quality of life. Low-level laser therapy (LLLT) uses infrared or near-infrared light to produce analgesic, anti-inflammatory, and biological stimulation effects. The aim of this systematic review is to evaluate the effect of LLLT on burning pain, quality of life, and negative emotions in patients with BMS. Methods The PubMed, Embase, Cumulative Index of Nursing and Allied Health Literature (CINAHL), Cochrane Library, Web of Science, and Scopus databases were searched up January 2023 to identify relevant articles. All randomized controlled trials that were published in English and examined the use of LLLT treatment for BMS were included. The methodological quality of the included trials was assessed using the Cochrane risk of bias tool for randomized controlled trials (RCTs). A meta-analysis was performed to evaluate burning pain, quality of life, and negative emotions. Sensitivity, subgroup, and funnel plot analyses were also carried out. Results Fourteen RCTs involving a total of 550 patients with BMS met the inclusion criteria. The results showed that LLLT (measured by the Visual Analog Scale; SMD: -0.87, 95% CI: -1.29 to -0.45, P

In order to identify a Lactobacillus sp. strain with efficient and safe in starch degradation, this study analyzed five distinct strains: Lactiplantibacillus plantarum JYI-3913 and AS1.555, Lactobacillus amylovorus CGMCC1.3395, Lactobacillus amylophilus CGMCC1.3394, and Lactobacillus paracasei CGMCC1.12731. The preliminary screening and re-screening for amylase production capabilities, the determination of enzyme production curve and growth curve were carried out to select the strain with the best ability to produce amylase. Subsequently, a detailed safety evaluation of the strain was carried out, including drug sensitivity test, drug resistance genetic testing, plasmid detection, hemolysis test, nitroreductase production, azoreductase production and amine production capacity detection, and acute oral toxicity test in mice. Results showed that, under the same culture conditions, the starch degradation transparent zone of L. amylovorus CGMCC1.3395 exhibited a clear transparent zone of starch degradation. Its starch degradation rate and amylase activity were 97.28% and 36.94 U/mL, respectively. And this strain displayed a relatively brief lag phase, while extending the log phase and stationary phase. Overall, this strain demonstrated superior integrated amylase production capabilities when compared to the other strains under investigation. In terms of antibiotic sensitivity, the selected strain exhibited sensitivity or intermediate sensitivity to the majority of the 22 tested antibiotics, with resistance observed for ampicillin, kanamycin, and four fluoroquinolone antibiotics. Moreover, this strain showed no associated drug-resistance genes and plasmids, thereby eliminating the risk of antibiotic-resistance gene transmission. It also lacked a hemolytic effect and did not produce potentially harmful metabolites such as nitroreductase, azoreductase, histamine, and tyramine. Mice displayed normal growth and weight gain over a 14 day feeding period, indicating the non-toxicity of the strain. In conclusion, L. amylovorus CGMCC1.3395 demonstrated a potent and safe capability for amylase production. These findings provide a theoretical foundation for the safe employment of this strain in the fermentation of starch-rich foods.

Rare-earth elements (REEs) are often highly concentrated in sedimentary phosphate deposits, and the microdistribution characteristics and occurrence state of rare earth in these deposits play a crucial role in the overall development and utilization of mineral resources. This study aims to analyze the microdistribution of REEs in REE-bearing phosphate deposits in the Zhijin region of Guizhou at the microstructural level and investigate their occurrence modes. Specifically, rock and mineral identification, X-ray diffraction (XRD), scanning electron microscopy–energy-dispersive X-ray spectroscopy (SEM-EDS), and laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) were utilized to analyze the samples. The correlation between the distribution of REEs and phosphorus was examined. In addition, the microdistribution of REEs in specific mineral phases and the locations of their occurrence were investigated. The analysis revealed that no REEs existed independently in the deposit. Instead, the distribution of REEs was highly consistent and significantly positively correlated with that of phosphorus. In the microarea structure, REEs were predominantly found both in particles, such as bioclasts, sand debris, and agglomerates, and in phosphate cement, where the main mineral components were collophane and apatite. Conversely, the content of REEs in dolomitized sand debris edges, sparry dolomitic cement, and siliceous cement was considerably lower. Based on these findings, it is speculated that REEs primarily occur within the lattice defects of apatite or on the surface of collophanite. There is a notable contrast in the REE content between the unaltered sand debris at the periphery and the dolomitized sand debris, indicating that the dolomitization in the diagenetic stage resulted in a depletion of REE abundance in the ore. Obviously, the dominant gangue mineral, dolomite, does not serve as the primary host for REEs. Furthermore, the highest concentration of REEs was inside organisms. This finding suggests that the high content of REEs in biological soft tissue may remain under the influence of waves and tides, and REE-bearing apatite may be preferentially separated and fill the cavities of deceased organisms. The second highest content of REEs was found in the shells of organisms, indicating that small shelly organisms absorb phosphorus materials through their life activities to construct their shells, resulting in REE enrichment. Quantitative analysis through sequential extraction procedures displayed that most REEs were present in the residual state, with a smaller portion combined with organic matter. These results confirm that REEs in the Zhijin phosphate deposits primarily exist as isomorphic substitutions in the lattice defects of apatite, with a secondary occurrence as organic matter-bound REEs.

Background: Atrial fibrillation (AF) is a common arrhythmia that can result in adverse cardiovascular outcomes but is often difficult to detect. The use of machine learning (ML) algorithms for detecting AF has become increasingly prevalent in recent years. This study aims to systematically evaluate and summarize the overall diagnostic accuracy of the ML algorithms in detecting AF in electrocardiogram (ECG) signals. Methods: The searched databases included PubMed, Web of Science, Embase, and Google Scholar. The selected studies were subjected to a meta-analysis of diagnostic accuracy to synthesize the sensitivity and specificity. Results: A total of 14 studies were included, and the forest plot of the meta-analysis showed that the pooled sensitivity and specificity were 97% (95% confidence interval [CI]: 0.94–0.99) and 97% (95% CI: 0.95–0.99), respectively. Compared to traditional machine learning (TML) algorithms (sensitivity: 91.5%), deep learning (DL) algorithms (sensitivity: 98.1%) showed superior performance. Using multiple datasets and public datasets alone or in combination demonstrated slightly better performance than using a single dataset and proprietary datasets. Conclusions: ML algorithms are effective for detecting AF from ECGs. DL algorithms, particularly those based on convolutional neural networks (CNN), demonstrate superior performance in AF detection compared to TML algorithms. The integration of ML algorithms can help wearable devices diagnose AF earlier.

To explore the causal pathogen and the correlated rhizosphere soil microecology of sugarcane root rot, we sampled the sugarcane root materials displaying different disease severity, and the corresponding rhizosphere soil, for systematic root phenotype and microbial population analyses. We found that with increased level of disease severity reflected by above-ground parts of sugarcane, the total root length, total root surface area and total volume were significantly reduced, accompanied with changes in the microbial population diversity and structure in rhizosphere soil. Fungal community richness was significantly lower in the rhizosphere soil samples from mildly diseased plant than that from either healthy plant, or severely diseased plant. Particularly, we noticed that a peculiar decrease of potential pathogenic fungi in rhizosphere soil, including genera Fusarium, Talaromyces and Neocosmospora, with increased level of disease severity. As for bacterial community, Firmicutes was found to be of the highest level, while Acidobacteria and Chloroflexi of the lowest level, in rhizosphere soil from healthy plant compared to that from diseased plant of different severity. FUNGuild prediction showed that the proportion of saprophytic fungi was higher in the rhizosphere soil of healthy plants, while the proportion of pathogenic fungi was higher in the rhizosphere soil of diseased plants. By co-occurrence network analysis we demonstrated the Bacillus and Burkholderia were in a strong interaction with Fusarium pathogen(s). Consistently, the biocontrol and/or growth-promoting bacteria isolated from the rhizosphere soil were mostly (6 out of 7) belonging to Bacillus and Burkholderia species. By confrontation culture and pot experiments, we verified the biocontrol and/or growth-promoting property of the isolated bacterial strains. Overall, we demonstrated a clear correlation between sugarcane root rot severity and rhizosphere soil microbiome composition and function, and identified several promising biocontrol bacteria strains with strong disease suppression effect and growth-promoting properties.

Al/Fe bimetals prepared by a compound casting method, combining the excellent properties of both the Al alloy and the ductile cast iron, exhibit great potential for application in achieving engine weight reduction. However, the problem of insufficient interfacial bonding ability because of the difference in thermophysical properties of Al and Fe is particularly prominent. Therefore, in this work, the electrodeposited Cu coating on the surface of the Fe matrix was used as the interlayer of Al/Fe bimetal fabricated by coupling hot-dipping with compound casting to solve the above problem. The effect of Cu interlayer thickness on the interfacial microstructure and shear strength of bimetal was investigated. The experimental results showed that the shear strength up to 77.65 MPa in regard to Al/Fe bimetal with a 5 μm Cu interlayer was obtained. No Cu element was detected at the interface of bimetal regardless of the thickness of the Cu interlayer. The diffusion behavior of the Cu atom at the interface and the influence of the Cu layer at the atomic scale on diffusion reaction and the Al/Fe interface were further revealed by combining first-principle and molecular dynamics calculations. The simulation results revealed that the Cu layer gradually dissolved into an Al alloy at 750 °C, thereby promoting the diffusion reaction of the Al/Fe interface. Meanwhile, the protective role of the Cu layer against oxidation on the surface of the Fe matrix was confirmed. As a result, the interfacial bonding performance was enhanced when the Cu interlayer was introduced.

Hong Qu (HQ), obtained through fermentation of various grains using Monascus spp., has been widely utilized as the main and characteristic initial saccharification and traditional fermentation starter in the food brewing industry. The quality, color, and flavor of HQ and HQ wine are closely related to the saccharifying power (SP) and Monascus pigments (MPs) of Monascus spp. In this study, to optimize the culture medium in submerged fermentation by M. purpureus G11 for improving SP and MPs, the effects of carbon source, nitrogen source, inorganic salts, and vitamins on SP activity and biosynthesis of MPs were explored through single-factor analysis and response surface Box–Behnken experiments. The results showed that the optimal medium composition was 6.008% rice powder, 1.021% peptone, 0.0049% CuSO4, and 0.052% vitamin B1. Validation experiments performed under the optimized fermentation conditions showed a significant increase in MPs and SP by 14.91% and 36.24%, with maximum MPs and SP reaching 112.61 and 365.12 u/mL, respectively. This study provides a theoretical basis for enhancing MPs and SP in M. purpureus for HQ production, to improve the production efficiency and shorten the production cycle of HQ-related fermentation products.

High-entropy alloys (HEAs) with excellent mechanical properties have broad application scope and application prospects. However, it is difficult to obtain the optimized element composition, based on the traditional equiatomic or near-equiatomic statistical analysis of the phase selection rules. The non-equiatomic HEAs have abundant constituents combination by optimizing the type and content of elements. In this study, Ni10Cr6WFe9TiAlx (x = 0, 1.0 and 1.5, at.%) HEAs were prepared by vacuum arc melting. The effect of Al content x on microstructure and mechanical properties of HEAs was systematically studied. The results show that the HEAs are composed mainly of face-centered cubic (FCC) with hexagonal Al2W phase. The increase of Al content promotes the formation of the hexagonal Al2W phase. When the Al mole content is 1.0, the Ni10Cr6WFe9TiAl HEA material has achieved superior mechanical properties. The alloy exhibited a high ultimate tensile strength of 741 MPa and a large total elongation of 46%. The improvement in the mechanical properties of the Ni10Cr6WFe9TiAl HEA is mainly attributed to the precipitation strengthening of the high-density Al2W phase. This work provides a reference for the future design of Al2W precipitation-strengthened non-equiatomic HEAs with ideal properties.

Monascus pigments (MPs) display many beneficial biological activities and have been widely utilized as natural food-grade colorants in the food processing industry. The presence of the mycotoxin citrinin (CIT) seriously restricts the application of MPs, but the gene regulation mechanisms governing CIT biosynthesis remain unclear. We performed a RNA-Seq-based comparative transcriptomic analysis of representative high MPs-producing Monascus purpureus strains with extremely high vs. low CIT yields. In addition, we performed qRT-PCR to detect the expression of genes related to CIT biosynthesis, confirming the reliability of the RNA-Seq data. The results revealed that there were 2518 differentially expressed genes (DEGs; 1141 downregulated and 1377 upregulated in the low CIT producer strain). Many upregulated DEGs were associated with energy metabolism and carbohydrate metabolism, with these changes potentially making more biosynthetic precursors available for MPs biosynthesis. Several potentially interesting genes that encode transcription factors were also identified amongst the DEGs. The transcriptomic results also showed that citB, citD, citE, citC and perhaps MpigI were key candidate genes to limit CIT biosynthesis. Our studies provide useful information on metabolic adaptations to MPs and CIT biosynthesis in M. purpureus, and provide targets for the fermentation industry towards the engineering of safer MPs production.

The formation of underground stem bulblets in lilies is a complex biological process which is key in their micropropagation. Generally, it involves a stem-to-bulblet transition; however, the underlying mechanism remains elusive. It is important to understand the regulatory mechanism of bulblet formation for the reproductive efficiency of Lilium. In this study, we investigated the regulatory mechanism of underground stem bulblet formation under different conditions regarding the gravity point angle of the stem, i.e., vertical (control), horizontal, and slanting. The horizontal and slanting group displayed better formation of bulblets in terms of quality and quantity compared with the control group. A transcriptome analysis revealed that sucrose and starch were key energy sources for bulblet formation, auxin and cytokinin likely promoted bulblet formation, and gibberellin inhibited bulblet formation. Based on transcriptome analysis, we identified the LoLOB18 gene, a homolog to AtLOB18, which has been proven to be related to embryogenic development. We established the stem bud growth tissue culture system of Lilium and silenced the LoLOb18 gene using the VIGS system. The results showed that the bulblet induction was reduced with down-regulation of LoLOb18, indicating the involvement of LoLOb18 in stem bulblet formation in lilies. Our research lays a solid foundation for further molecular studies on stem bulblet formation of lilies.

In this paper, a novel nonsingular finite-time backstepping controller is constructed for trajectory tracking of marine vessel subject to unknown external disturbances. Firstly, in the presence of disturbances, a disturbance observer (DO) is proposed to estimate and compensate the disturbances exactly in finite time. Secondly, a finite-time tracking controller is designed in the classical backstepping procedure, however, the inevitable singularity appears in calculating the derivative of virtual control. Furthermore, for overcoming this singularity, a nonsingular finite-time backstepping controller is designed by adopting a finite-time command filter to estimate the derivative, instead of calculating it directly. Theoretical analysis demonstrates the closed-loop system is finite-time stable. Finally, simulation results and comparisons illustrate the effectiveness of the proposed method.

A new method is proposed to solve edge cracking and corrosion in rolling process of the magnesium alloy plate. Usually in the process, the edge cracking is caused by the great temperature difference between the edge and the center of the alloy plate and the corrosion is by water in air. The method uses hot air wrapped around the plate to perfectly prevent edge cracking and corrosion. The method also includes cathodic protection for the magnesium alloy based on electrochemistry. The suppression of cathodic protection on the chemical activity of the magnesium alloy was studied. The experiment shows that the new method of edge heating with hot air wrapping is effective to prevent edge cracking and corrosion. Also, the cathodic protection can effectively suppress the chemical activity of the magnesium alloy and improve the surface quality in the rolling process of the magnesium plate, which provides a new concept in rolling process of the magnesium alloy.

Rice sheath blight, caused by Rhizoctonia solani, is one of the major rice diseases. In order to better understand the inhibitory mechanism of lauric acid on the disease, RNA sequencing (RNA-Seq) was used to analyze the transcriptome changes in Rhizoctonia solani treated with lauric acid for 3 h, 6 h, 18 h, and 24 h, including 2306 genes; 1994 genes; 2778 genes; and 2872 genes. Based on gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, we found that protein processing in endoplasmic reticulum (KO04141), carbon metabolism (KO01200), and starch and sucrose metabolism were significantly enriched. Most oxidoreductase, dehydrogenase, reductase, and transferase genes are downregulated in this process. Lauric acid can affect ergosterol content, mitochondrial membrane potential collapse, hydrogen peroxide content, electrolyte leakage, reactive oxygen species balance, and can induce endoplasmic reticulum (ER) stress. Lauric acid also increased the expression levels of ER chaperone glucose regulatory protein Grp78 (BIP), protein disulfide isomerase (PDI), and Calpain (CNX), and decreased the expression levels of HSP40, HSP70, and HSP90 genes. Lauric acid affected the ergosterol content in the cell membrane of R. solani, which induces ER stress and increases the BiP level to induce the apoptosis of Rhizoctonia solani. These results indicated that lauric acid could be used to control rice sheath blight.

Ultrasonic-assisted grinding processing can effectively reduce the surface roughness and enhance the processing efficiency in the processing of hard and brittle materials. However, the most common ultrasonic assisted grinding is a type of contact ultrasonic grinding where the grinding tool directly contacts the workpiece, which means that it is necessary to accurately control the pre-pressure of the grinding tool on the workpiece. The control of pre-pressure will inevitably increase the complexity of the grinding device, and it is easy to wear the workpiece because of improper pre-pressure control. In this paper, a non-contact ultrasonic grinding method is proposed and the machining mechanism of non-contact ultrasonic grinding is revealed. The resonant frequency of the ultrasonic vibration system and vibration amplitude of the grinding tool working face were simulated and experimentally tested, respectively. Then, the experiment of non-contact ultrasonic grinding of a sapphire wafer was carried out. The result showed that non-contact ultrasonic grinding of the sapphire wafer could reduce the surface roughness by 48.6%. Compared with traditional contact grinding of sapphire wafer under certain pre-pressure conditions, the experimental results show that non-contact ultrasonic grinding has better effects in reducing surface roughness, improving processing efficiency, and improving the quality uniformity of the workpiece machining surface.

Coix lacryma-jobi L. is an important minor cereal with a high nutritional and medicinal value in Asian countries. The hilly region of South China is the secondary center of diversity of Coix lacryma-jobi L. In the present study, we took a sample of 139 Coix lacryma-jobi L. genotypes from four geographical regions in Southwest China and analyzed the genetic diversity and population structure using AFLP markers. Six primer combinations detected a total of 743 (89.52%) polymorphic loci. The percentage of polymorphic bands within the four geographical populations ranged from 56.02% (Guangxi) to 86.75% (Guizhou). The overall genetic diversity of 139 Coix lacryma-jobi L. was relatively low (h ranged from 0.1854 to 0.2564). The neighbor-joining method grouped all Coix lacryma-jobi L. genotypes into two clusters with no geographical affinity observed among genotypes within the same group. The Fst indicated the two clusters existed great genetic differentiation. AMOVA analysis showed the molecular variation within populations was much higher than that among populations of geographical regions and subpopulations derived from STRUCTURE. Human activities and the natural outcrossing system of Coix lacryma-jobi L. may have a great influence on its distribution, genetic diversity, and population structure. Our study provides useful information for local breeding programs of Coix lacryma-jobi L. as well.

Plant diseases reduce crop yield and quality, hampering the development of agriculture. Fungicides, which restrict chemical synthesis in fungi, are the strongest controls for plant diseases. However, the harmful effects on the environment due to continued and uncontrolled utilization of fungicides have become a major challenge in recent years. Plant-sourced fungicides are a class of plant antibacterial substances or compounds that induce plant defenses. They can kill or inhibit the growth of target pathogens efficiently with no or low toxicity, they degrade readily, and do not prompt development of resistance, which has led to their widespread use. In this study, the growth inhibition effect of 24 plant-sourced ethanol extracts on rice sprigs was studied. Ethanol extract of gallnuts and cloves inhibited the growth of bacteria by up to 100%. Indoor toxicity measurement results showed that the gallnut and glove constituents inhibition reached 39.23 μg/mL and 18.82 μg/mL, respectively. Extract treated rice sprigs were dry and wrinkled. Gallnut caused intracellular swelling and breakage of mitochondria, disintegration of nuclei, aggregation of protoplasts, and complete degradation of organelles in hyphae and aggregation of cellular contents. Protection of Rhizoctonia solani viability reached 46.8% for gallnut and 37.88% for clove in water emulsions of 1000 μg/mL gallnut and clove in the presence of 0.1% Tween 80. The protection by gallnut was significantly stronger than that of clove. The data could inform the choice of plant-sourced fungicides for the comprehensive treatment of rice sprig disease. The studied extract effectively protected rice sprigs and could be a suitable alternative to commercially available chemical fungicides. Further optimized field trials are needed to effectively sterilize rice paddies.

The load simulation test bench plays an important role in tracked vehicle development. The stability and accuracy of the system have a vital impact on the experimental results. To accurately reproduce the power performance of a tracked vehicle on the test platform, this paper aims to investigate the model, control, and calibration method of the test bench. Firstly, the dynamic model of a tracked vehicle under complex driving conditions is analyzed and established, which takes driving torque as the input and driving wheel speed as the output. Then, considering the uncertainties and disturbances in the system model, a 2-degree-of-freedom (2-DOF) control method combined with a disturbance observer is proposed to solve the stability problem of the system. Furthermore, in order to investigate the accuracy of the simulation on the test bed, a method of calibrating the system by a flywheel set with standard inertia is proposed. In the calibration process, the influence of the system resistance torque and the original mechanical inertia on the results is considered, and the response time of the inertia simulation is analyzed in both a steady and dynamic state. Finally, the load simulation test is carried out with the corrected system. The test results show that the system has a high load simulation accuracy under various load simulation tests.