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Background/purpose Patients with periodontal disease have higher dental fear levels, which may have negative effects on their clinical outcome during scaling and root planing (SRP). The present study used the new classification of periodontitis and validated questionnaires to assess the relationship among dental fear, SRP pain and periodontal status. Materials and methods A total of 120 periodontitis patients were enrolled and staging according to the new classification of periodontitis. SRP was performed, and the visual analog scale (VAS) to assess pain was used with every patient after treatment. Questionnaires, including Corah's Dental Anxiety Scale (DAS), Dental Fear Survey (DFS), and short-form Dental Anxiety Inventory (S-DAI) were implemented from the first attendance and subsequent visits after 6 months. The patients were grouped by DAS scores. The statistical analysis was performed using T-test, chi-square, Pearson and Spearman correlative analysis. Results Compared to pre-SRP treatment, the dental fear level on DFS was decreased in the posttreatment period for all periodontitis stages. There were no statistically significant differences in S-DAI and DAS between pretreatment and posttreatment periods in stage I and II; meanwhile, there were statistically differences in stage III and IV. The correlation among periodontitis stages, VAS and dental fear level was significant. The proportion of high periodontitis stages was increased in high dental fear group. Conclusion SRP can reduce dental fear levels in all periodontitis stages, especially in stage III and IV. Correlations exist among periodontal status, dental fear and SRP pain. High dental fear is associated with poor periodontal status.

Background/purpose In the immediate implantation of maxillary central incisors, the height of the alveolar bone is lost, and there is often a risk of bone fracture due to the thin buccal bone wall (BBW). The purpose of this study was to assess the effects of smoking, age, and root position in the alveolar bone on the BBW and the distance between the cemento-enamel junction (CEJ) and the facial bone crest (FBC) of Chinese maxillary central incisors. Materials and methods The patients were divided by smoking, gender, age, and root sagittal position in the alveolar bone. BBW thickness was measured at the following sites: the 4 mm apical to the CEJ, the middle of the root, and the apex. The distance from the CEJ to the FBC was also evaluated. Results Cone beam CT (CBCT) data for the maxillary central incisors of 645 patients (323 males and 322 females) were selected and analyzed. The CEJ-FBC distance in patients who smoked (2.79 ± 0.78 mm) was significantly greater than that of non-smokers (2.54 ± 0.69 mm). The BBW in subtype III (0.74 ± 0.43 mm, 0.81 ± 0.36 mm) was thinner than that in subtypes I and II at 4 mm apical to the CEJ and in the middle of the root, with a statistically significant difference (p Conclusion In most Chinese people, smoking, gender, age, and the position of the root in alveolar bone are all important factors that must be considered before immediate implantation is undertaken.

Background: The present study used the new classification of periodontitis and validated questionnaires to assess the relationship among dental fear, scaling and root planning (SRP) pain and periodontal status for clinical evaluation. Methods: One hundred and twenty periodontitis patients were enrolled for retrospective analysis and staging according to the new classification of periodontitis. All patients included in this study from July 2018 to January 2020 were divided into periodontitis stages. Scaling and root planning (SRP) was performed and Visual analogue scale (VAS) was determined for every patient immediately after SRP. Application of questionnaires including Corah’s Dental Anxiety Scales (DAS), Dental Fear Survey (DFS), and the short-form Dental Anxiety Inventory (S-DAI) were implemented from the first attendance and consequent visits after 6 months. All patient demographic data were collected including age, gender, marital status and education level. The scores of each dental fear scale and combination scales were also recorded. Patients were further subdivided into two categories based on DAS scores (low dental fear group: DAS

Background: The present study used the new classification of periodontitis and validated questionnaires to assess the relationship among dental fear, SRP pain and periodontal status for clinical evaluation.Methods: An amount of 120 periodontitis patients were retrospective analyzed and staged according to the new classification of periodontitis. Scaling and root planning (SRP) was performed and Visual analogue scales (VAS) applied immediately after SRP treatment. Application of questionnaires including Corah’s Dental Anxiety Scales (DAS), Dental Fear Survey (DFS), and the short-form Dental Anxiety Inventory (S-DAI) were adopted at the time of first attendance and consequent visit after 6 months to assess dental fear level. The scores of each dental fear scale and combination scales were recorded. Patients were divided into two categories according to DAS value (low dental fear group: DASResults: Compared to pre-SRP treatment, dental fear level of DFS and combination scales were significantly decreased in post-treatment in all periodontitis stage. There were no statistical significance on dental fear level of S-DAI and DFS between patients pre and post-treatment in periodontitis stage I and II, while statistical significance were shown in stage III and IV. Correlation were statistical significance among dental fear level assessed by DAS, DFS and S-DAI, VAS and periodontitis stages. Significant differences of patient amount were shown between two categories according to DAS.Conclusions: SRP can reduce dental fear level in all periodontitis stages, especially stage III and IV. Correlations exist among periodontal status, dental fear and SRP pain. High dental fear relates to poor periodontal status.

Tidal channel networks are ubiquitous features of coastal landforms that control the input and output of intertidal water, sediment and nutrients. Nevertheless, those intertidal platforms have undergone extensive losses due to human activities such as land reclamation. Identifying how tidal channel networks respond to land reclamation is critical to our prediction of the fate of residual tidal landforms. However, the morphological changes in the channel networks in the Yellow River Delta (YRD) impacted by severe reclamation remain unclear. Here, we analyzed the spatiotemporal dynamics of channel networks on two scales (the delta scale and the zone scale) under the double stress of land reclamation and natural pressure by comparing a comprehensive suite of morphological channel characteristics, including channel segment count, channel order, length, fractal dimension, drainage density and drainage efficiency. The results show that the interannual dynamics of tidal channel networks in the delta over the last three decades have experienced two periods: a favorable period during 1984-2000 and an adverse period during 2001-2018. The spatiotemporal patterns of channel networks varied with zones. Land reclamation exerted a dominant influence on shifting the evolutionary trend of channel networks on both the delta scale and the zone scale when reclamation proportion exceeded a certain threshold. Sediment siltation could to a certain degree mitigate the impact of reclamation on tidal channel networks development. Our study highlighted the effect of reclamation on the geomorphological evolution of tidal channel networks and identified its impact threshold which could further be used to guide coastal zone restoration and management.

Reclamation of coastal wetlands causes the loss of multiple ecosystem services, necessitating that measures to address these impacts be taken. Offset ratios are commonly used to determine the extent of habitat areas that need to be restored elsewhere to achieve no net loss in wetlands; however, most calculation methods focus on individual impacts. In this study, a mathematical model accounting for multiple ecosystem services and their synergies or trade-offs relationships was developed to determine the required allocation of compensation area, and optimal amount of offsetting based on calculated ratios between damaged and compensated habitat areas. We took the Yellow River Delta in China as a case study, calculated the impacts on ecosystem services of different coastal reclamation types, and quantified the offset ratios for restoration based on different compensation scenarios accounting for time lags. We estimated that the offset ratios for the conversion of coastal wetlands into mariculture were smallest among all the reclamation types. We showed that compensating for carbon storage and water regulation with trade-offs will require higher offset ratios than those based on biodiversity only, and will also be more flexible. Our findings suggested that trade-offs and synergetic relationships should be prioritized when making decisions regarding the restoration of multiple ecosystem services. The findings presented herein will facilitate design of biodiversity offsets for coastal land reclamation based on their different impacts on ecosystem services. Overall, the results of this study have important implications for the ecological restoration and compensation of coastal wetlands in the face of coastal land reclamation.

River deltas contain complex self-organizing channel networks that continuously exchange fluxes of water, matter, energy, and information with their surroundings. The connectivity of these exchange processes plays a crucial role in controlling the evolution and dynamic stability of river deltas. However, connectivity patterns related to tidal channel networks have rarely been studied, especially in the Yellow River Delta (YRD), which is impacted by severe reclamation. Here, we evaluated the potential hydrological connectivity dynamics between the tidal channel network and its surroundings using an index of connectivity (IC) in the whole YRD and its three sub-regions: erosion zone, oil field zone and deposition zone. The results suggested that different areas had different spatial connectivity potential. The mean value of the IC related to the channel networks showed little difference for any zones. However, the total connectivity response area (CRA; set of connectivity response units) varied with the study scale. A decreasing trend was found on the delta scale and a relatively stable trend was found in the deposition zone. In terms of dynamic connectivity, the tidal flat system did not show a continuous trend over time. Our results indicated that the YRD is such a dynamic complex that a relatively stable connectivity pattern is unlikely to be achieved over time. Therefore, future ecological restoration based on hydrological connectivity needs to consider more related influencing factors and their temporal and spatial dynamics.

In order to solve the decision-making problem of taxi drivers at the airport, it constructed a non-aggregate stochastic utility model, a cell transmission simulation model, and a queuing model. Besides, it used software programming and drawing software such as MATLAB, SPSS, EXCEL, and rapid flow chart comprehensively. Finally, this paper solves the taxi problem at the airport and gives relevant suggestions.

Massive die-off in salt marshes is one of the most common examples of widespread degradation in marine and coastal ecosystems. In salt marshes, tidal channel networks facilitate the exchange of water, nutrients, sediments and biota with the open marine environments. However, quantitative analyses of the role of channel networks in alleviating vegetation die-off in salt marshes are scarce. Here we quantified the spatial-temporal development of marsh vegetation die-off in the northern Liaodong Bay by analyzing aerial images before, during, and after a drought (from 2014 to 2018). We found that Suaeda salsa marshes have recently experienced large-scale die-off. The extent of vegetation die-off increases with increasing distance from the channel network. Moreover, our results suggested that efficient tidal channel networks (high drainage density, low mean unchanneled path length) can mitigate die-off at the watershed scale. We presented possible abiotic & biotic processes in channel networks that explain this spatial dynamic. Our study highlights the importance of efficient tidal channel networks in mitigating die-off and enhancing the resistance of marshes to droughts, and call for incorporating theses dynamics in coastal restoration and management.

Die-off of coastal wetlands has been reported worldwide. Planting habitat-forming species is an important strategy to reverse the decline of coastal wetlands. However, how abiotic environmental stresses and consumers affect the establishment of the planted vegetation species is unclear. We reported a large-scale restoration project in the Liaohe estuary, China, where native pioneer plant Suaeda salsa was planted. We evaluated the growth performance of the planted S. salsa, and identified the constraints on the establishment of planted S. salsa. Results showed that the growth performance (density, coverage and survival rate) of planted S. salsa was better in the low restored marsh than that in the high restored marsh. The death of planted S. salsa was primarily driven by crab herbivory, followed by abiotic stresses (low soil moisture and high salinity) in the high restored marsh, whereas plant death was only driven by crab herbivory in the low restored marsh. Herbivory strength in the high marsh was significantly higher than that in the low marsh. Our findings challenge the bottom-up paradigm used as the foundation for coastal restoration, and highlight the overlooked role of consumers. Therefore, protection measures against consumer pressure, especially in physically harsh conditions, should be considered to enhance the success of coastal wetland restoration.

Background Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker. Results The synthesized gene of CgA coding area was cloned and expressed as fusion protein CgA-His in procaryotic system. Then the purified CgA-His protein was mixed with QuickAntibody-Mouse5W adjuvant, and injected into mice. The CgA-His protein was also used as coating antigen to determine the antiserum titer. By screening, a stable cell line named 4E5, which can generate anti-CgA monoclonal antibody (mAb), was obtained. The isotype of 4E5 mAb was IgG2b, and the chromosome number was 102 ± 4. Anti-CgA mAb was purified from ascites fluid, and the affinity constant reached 9.23 × 109 L/mol. Furthermore, the specificity of the mAb was determined with ELISA, western blot and immunohistochemistry. Results indicated that the mAb 4E5 was able to detect chromogranin A specifically and sensitively. Conclusions A sensitive and reliable method was successfully developed for rapid production of anti-CgA mAb for immunohistochemistry diagnosis in this study, and the current study also provides conclusive guidelines for preparation of mAbs and implements in immunohistochemistry diagnosis.

Podoplanin (PDPN), a 38 kDa transmembrane sialoglycoprotein from human, is expressed in lymphatic endothelial cells but not in vascular endothelial cells, and has been considered as a specific marker of lymph. In this study, the gene encoding the extracellular part of PDPN (ePDPN) was synthesized and used to expressed fusion protein ePDPN-His and GST-ePDPN, respectively, in E.coli. The purified GST-ePDPN fusion protein was mixed with QuickAntibody-Mouse5W adjuvant to immune mice, and the antiserum titer was determined by indirect ELISA. A stable cell line named 5B3 generating anti-PDPN monoclonal antibody (mAb) was obtained by hybridoma technology. The isotype of 5B3 cell line was IgG2b, and the chromosome number was 102 ± 4. The 5B3 mAb was purified successfully from ascites fluid through Protein G column, and its affinity constant was 2.94 × 108 L/mol. Besides, excellent specificity of the 5B3 mAb was further demonstrated in ELISA, western blot and immunohistochemistry experiments, suggesting that 5B3 mAb displays similar application value to D2-40, a commercial available antibody. Hence, the current study provides conclusive guidelines for preparation of other mAbs and their applications in immunohistochemistry diagnosis.

BackgroundHuman B-cell lymphoma 6 (BCL6) gene, usually coding protein of 706 amino acids, is closely associated with large B cell lymphoma. Researches showed that protein mutation or change of expression levels usually happened in the mounting non-hodgkin lymphoma (NHL). Thus BCL6 is considered to be involved in germinal center (GC)-derived lymphoma.ResultsThe BCL61-350 gene codons were optimized for prokaryotic system. After expression of BCL61-350 in E. coli, the BCL61-350 protein was purified with Ni column. Then the BCL61-350 protein, mixing with QuickAntibody-Mouse5W adjuvant, was injected into Balb/c mice. After immunization and cell fusion, a stable cell line named 1E6A4, which can secrete anti-BCL6 antibody, was obtained. The isotype of 1E6A4 mAb was determined as IgG2a, and the affinity constant reached 5.12×1010 L/mol. Furthermore, the specificity of the mAb was determined with ELISA, western blot and immunohistochemistry. Results indicated that the 1E6A4 mAb was able to detect BCL6 specifically and sensitively.ConclusionsBCL61-350 antigen has been successfully generated with an effective and feasible method, and a highly specific antibody named 1E6A4 against BCL6 has been screened and characterized in this study, which was valuable in clinical diagnosis.

Objectives: Diabetics significantly increase risk for periodontitis. Interleukin-6 (IL-6) gene polymorphism may play certain roles in the progression of periodontitis with diabetes. The purpose of this study was to assess the association among IL-6 gene polymorphisms, type 2 diabetes mellitus (T2DM) and chronic periodontitis (CP) in a Chinese population. Material and methods: DNA was obtained from 159 patients with CP, 88 patients with T2DM, 110 patients with CP&T2DM and 135 control subjects. The -174/-572/-597 polymorphisms of IL-6 gene were investigated by restriction fragment length polymorphism of polymerase chain reaction products. The results were further confirmed by sequencing. Significance was set at P

Chronic periodontitis (CP) exhibits inflammation and alveolar bone loss, and severe forms of periodontitis are suggested to have a genetic basis. Vitamin D receptor (VDR) regulates bone metabolism and inflammation-related genes, and single nucleotide polymorphisms (SNPs) in the VDR gene may affect the functional activity of the VDR protein in CP. Therefore, the aim of this study was to investigate the association between VDR SNPs and severe CP in a Chinese population.DNA was obtained from 107 patients with severe CP and 121 control subjects. The BsmI, TaqI, ApaI, and FokI SNPs of VDR genes were investigated by restriction fragment length polymorphism of polymerase chain reaction (PCR) products. The digested PCR products were electrophoresed on an 8% polyacrylamide gel and developed by the DNA silver staining method.The distribution of VDR TaqI genotypes and alleles between the two groups was significantly different (P = 0.019 and P = 0.039, respectively). The TT genotype and T allele seemed to increase the susceptibility of severe CP (odds ratio, 2.75; 95% confidence interval [CI]: 1.17 to 6.47; odds ratio, 2.28, 95% CI: 1.02 to 5.06, respectively) in Chinese populations. There was no significant difference in the genotype distribution or the allele frequencies of VDR BsmI, ApaI, or FokI between the two groups.TaqI SNP of VDR gene might be associated with severe CP in Chinese patients.

Aim: Several common single nucleotide polymorphisms (SNPs) of the cyclooxygenase-2 (COX-2) gene have been reported to be functional. The association between −1195GA, −765GC and 8473TC of COX-2, and severe chronic periodontitis (CP) in a Chinese population was investigated. Material and Methods: 148 cases of healthy controls (control group) and 146 cases of severe CP were recruited in this study. Genotypes of −1195GA, −765GC and 8473TC were determined by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The distributions of genotypes and haplotypes were compared by χ2 test and the odds ratios (ORs) were calculated by logistic regression analysis. Results: The prevalence of the −1195A was more prevalent in CP group (60.62%) than control group (51.35%), and the distributions of the −765C and 8473C were higher in control group (6.76% and 21.96%) compared with CP group (3.08% and 15.07%). Only genotype distribution of −1195GA was significant when p-value was corrected for multiple testing (pc=0.033). The adjusted ORs for the −1195AA/GA, −765GC and 8473CC/TC were 2.49 (95% CI=1.33–4.69, p=0.005), 0.45 (95% CI=0.20–1.04, p=0.061) and 0.67 (95% CI=0.41–1.11, p=0.118). Subjects with the haplotype AGT had a significantly higher risk of periodontitis than those with the most common haplotype GGT (OR=1.91, 95% CI=1.32–2.76, pc

Quantum neural network (QNN) is a promising area in the field of quantum computing and quantum information processing. A model of feedback quantum neuron (FQN) is proposed and its property is analyzed. A novel multi-user detector based on FQN and quantum register is proposed. An analysis is given to the theoretical basis and practical performance of the proposed detector. The simulation results show that the proposed detector has more powerful properties both in bit error rate and near-far resistance than Hopfield neural network based detector. A multi-user detector based on improved FQN is also proposed and the simulation results have shown that the improved FQN multi-user detector is obviously superior to the FQN multi-user detector. Its performance is closer to optimal, compared with the other multi-user detector.