129 results on '"Cheng-Fang Wang"'
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2. Generation and application of patient-derived xenograft models in pancreatic cancer research
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Cheng-Fang Wang, Xian-Jie Shi, and Yuan-Yuan Ji
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Medicine - Abstract
Abstract. Objective:. Pancreatic ductal adenocarcinoma cancer (PDAC) is one of the leading causes of cancer-related death worldwide. Hence, the development of effective anti-PDAC therapies is urgently required. Patient-derived xenograft (PDX) models are useful models for developing anti-cancer therapies and screening drugs for precision medicine. This review aimed to provide an updated summary of using PDX models in PDAC. Data sources:. The author retrieved information from the PubMed database up to June 2019 using various combinations of search terms, including PDAC, pancreatic carcinoma, pancreatic cancer, patient-derived xenografts or PDX, and patient-derived tumor xenografts or PDTX. Study selection:. Original articles and review articles relevant to the review's theme were selected. Results:. PDX models are better than cell line-derived xenograft and other models. PDX models consistently demonstrate retained tumor morphology and genetic stability, are beneficial in cancer research, could enhance drug discovery and oncologic mechanism development of PDAC, allow an improved understanding of human cancer cell biology, and help guide personalized treatment. Conclusions:. In this review, we outline the status and application of PDX models in both basic and pre-clinical pancreatic cancer researches. PDX model is one of the most appropriate pre-clinical tools that can improve the prognosis of patients with pancreatic cancer in the future.
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- 2019
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3. Chemical Composition and Bioactivities of the Essential Oil from Etlingera yunnanensis against Two Stored Product Insects
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Shan-Shan Guo, Chun-Xue You, Jun-Yu Liang, Wen-Juan Zhang, Zhu-Feng Geng, Cheng-Fang Wang, Shu-Shan Du, and Ning Lei
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Etlingera yunnanensis ,contact activity ,repellency ,composition ,stored product insects ,Organic chemistry ,QD241-441 - Abstract
The chemical composition of the essential oil of Etlingera yunnanensis rhizomes and its contact and repellent activities against Tribolium castaneum (Herbst) and Liposcelis bostrychophila (Badonnel) were investigated. The essential oil obtained from E. yunnanensis rhizomes with hydrodistillation was performed by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. The main components of the essential oil were identified to be estragole (65.2%), β-caryophyllene (6.4%), 1,8-cineole (6.4%), limonene (5.2%), and α-pinene (2.4%). It was found that the essential oil of E. yunnanensis rhizomes possessed contact toxicity against T. castaneum and L. bostrychophila (LD50 = 23.33 μg/adult and LD50 = 47.38 μg/cm2, respectively). Estragole, 1,8-cineole, and limonene exhibited stronger contact toxicity (LD50 values of 20.41, 18.86, and 13.40 μg/adult, respectively) than β-caryophyllene (LD50 = 41.72 μg/adult) against T. castaneum adults. Estragole possessed stronger contact toxicity (LD50 = 30.22 µg/cm2) than β-caryophyllene, 1,8-cineole, and limonene (LD50 values of 74.11, 321.20, and 239.62 μg/adult, respectively) against L. bostrychophila adults. Repellency of the crude oil was also evaluated. The essential oil and constituents possessed strong repellent activity against T. castaneum adults. The four individual constituents showed weaker repellent activity than the essential oil against L. bostrychophila adults. The results indicated that the essential oil of E. yunnanensis rhizomes and the individual constituents had the potential to be developed as a natural insecticide and repellent for the control of T. castaneum and L. bostrychophila.
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- 2015
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4. Chemical Composition and Insecticidal Activity of Essential Oils from Zanthoxylum dissitum Leaves and Roots against Three Species of Storage Pests
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Cheng-Fang Wang, Kai Yang, Chun-Xue You, Wen-Juan Zhang, Shan-Shan Guo, Zhu-Feng Geng, Shu-Shan Du, and Yong-Yan Wang
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Zanthoxylum dissitum ,essential oil ,contact activity ,stored product pests ,Organic chemistry ,QD241-441 - Abstract
This work aimed to investigate chemical composition of essential oils obtained from Zanthoxylum dissitum leaves and roots and their insecticidal activities against several stored product pests, namely the cigarette beetle (Lasioderma serricorne), red flour beetle (Tribolium castaneum) and black carpet beetle (Attagenus piceus). The analysis by GC-MS of the essential oils allowed the identification of 28 and 22 components, respectively. It was found that sesquiterpenoids comprised a fairly high portion of the two essential oils, with percentages of 74.0% and 80.9% in the leaves and roots, respectively. The main constituents identified in the essential oil of Z. dissitum leaves were δ-cadinol (12.8%), caryophyllene (12.7%), β-cubebene (7.9%), 4-terpineol (7.5%) and germacrene D-4-ol (5.7%), while humulene epoxide II (29.4%), caryophyllene oxide (24.0%), diepicedrene-1-oxide (10.7%) and Z,Z,Z-1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (8.7%) were the major components in the essential oil of Z. dissitum roots. The insecticidal activity results indicated that the essential oil of Z. dissitum roots exhibited moderate contact toxicity against three species of storage pests, L. serricorne,T. castaneum and A. piceus, with LD50 values of 13.8, 43.7 and 96.8 µg/adult, respectively.
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- 2015
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5. Radiosensitizing Effect of Schinifoline from Zanthoxylum schinifolium Sieb et Zucc on Human Non-Small Cell Lung Cancer A549 Cells: A Preliminary in Vitro Investigation
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Cheng-Fang Wang, Li Fan, Mei Tian, Xue-Song Qi, Jian-Xiang Liu, Jiang-Bin Feng, Shu-Shan Du, Xu Su, and Yong-Yan Wang
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schinifoline ,Zanthoxylum schinifolium ,radiosensitization ,A549 ,apoptosis ,cytofluorimetry ,Organic chemistry ,QD241-441 - Abstract
Schinifoline (SF), a 4-quinolinone derivative, was found in Zanthoxylum schinifolium for the first time. 4-Quinolinone moieties are thought to have cytotoxic activity and are often used as a tubulin polymerization inhibitors, heterogeneous enzyme inhibitors and antiplatelet agents. However, very little information respect to radiosensitization has focused on SF. This work aimed to investigate the radiosensitizing effect of SF on A549 cells. The cell viability results indicated cytotoxicity of SF on A549 cells, with IC50 values of 33.7 ± 2.4, 21.9 ± 1.9 and 16.8 ± 2.2 μg/mL, respectively, after 6, 12, 24 h treatment with different concentrations, and the 10% or 20% IC50 concentration during 12 h was applied in later experiments. The results of cell proliferative inhibition and clonogenic assay showed that SF enhanced the radiosensitivity of A549 cells when applied before 60Co γ-irradiation and this effect was mainly time and concentration dependent. The flow cytometric data indicated that SF treatment before the irradiation increased the G2/M phase, thus improving the radiosensitivity of A549, leading to cell apoptosis. This paper is the first study that describes the in vitro radiosensitising, cell cycle and apoptotic-inducing effects of schinifoline.
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- 2014
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6. Cytotoxic Compounds Isolated from Murraya tetramera Huang
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Chun-Xue You, Kai Yang, Cheng-Fang Wang, Wen-Juan Zhang, Ying Wang, Jiao Han, Li Fan, Shu-Shan Du, Zhu-Feng Geng, and Zhi-Wei Deng
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M. tetramera ,cytotoxicity ,coumarin ,sesquiterpene ,Organic chemistry ,QD241-441 - Abstract
A new compound and seven known compounds were isolated from Murraya tetramera Huang for the first time, and they were identified with NMR and MS spectral analysis. It was confirmed that the new compound was 10-methoxy-7-methyl-2H-benzo[g]chromen-2-one (3) and the others were β-eudesmol (1), trans-3β-(1-hydroxy-1-methylethyl)-8aβ-methyl-5-methylenedecalin-2-one (2), 5,7-dimethoxy-8-[(Z)-3'-methyl-butan-1',3'-dienyl]coumarin (4), 7-geranyloxy-6-methoxycoumarin (5), 5,7-dimethoxy-8-(3-methyl-2-oxo-butyl)coumarin (6), murrangatin acetate (7) and toddalenone (8). Furthermore, the cytotoxic activity against human lung adenocarcinoma (A549), human hepatocellular carcinoma cells (SMMC-7721), human bladder tumor cells (EJ), human cervical carcinoma cells (HeLa), and human B-lineage acute lymphoblastic leukemia 1 cells (BALL-1) was evaluated for all compounds. It was found that five of them displayed various degrees of cytotoxicity against different testing targets. Compound 1 showed significant cytotoxic activity against the five cell lines (A549, SMMC-7721, EJ, Hela and BALL-1). Compounds 2 and 5 showed significant cytotoxicity against three cell lines (A549, SMMC-7721 and BALL-1). Compound 4 showed significant cytotoxicity against three cell lines (A549, EJ and BALL-1). However, compound 3 only showed fair cytotoxicity against the BALL-1 cell line. The structure-active relationships were investigated as well. These active compounds might be potential lead compounds for the treatment of cancer.
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- 2014
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7. Insecticidal and Repellent Effects of the Essential Oils Extract from Zanthoxylum myriacanthum against Three Storage Pests
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Bo‐Ya Li, Jia‐Wei Zhang, Yu Zheng, Dan Wang, Cheng‐Fang Wang, and Shu‐Shan Du
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Molecular Medicine ,Bioengineering ,General Chemistry ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
8. Metabolic Dysfunction-Associated Fatty Liver Disease Increases the Risk of Gastroesophageal Reflux Symptoms
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Yuan He, Zhi-Jun Duan, Cheng-Fang Wang, Yu-Shan Wei, and Ming-Xu Cai
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Pharmacology ,obesity ,metabolic dysfunction-associated fatty liver disease ,questionnaire ,fungi ,Internal Medicine ,body mass index ,GERD ,gastroesophageal reflux symptoms ,Targets and Therapy [Diabetes, Metabolic Syndrome and Obesity] ,Original Research - Abstract
Yuan He,1,2 Zhi-Jun Duan,1 Cheng-Fang Wang,2 Yu-Shan Wei,3 Ming-Xu Cai2 1Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Peopleâs Republic of China; 2Healthcare Management Center, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Peopleâs Republic of China; 3Department of the Scientific Research Management, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Peopleâs Republic of ChinaCorrespondence: Zhi-Jun DuanDepartment of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, 116011, Peopleâs Republic of ChinaTel/Fax +86 41183635963Email duanzhijun12@outlook.com; cathydoctor@sina.comObjective: The present study aimed to explore the relationship between metabolic dysfunction-associated fatty liver disease (MAFLD) and gastroesophageal reflux symptoms (GERS).Methods: The present study was a cross-sectional observational study. The study population was 3002 subjects from a single hospital who underwent a health checkup from September 1, 2019, to December 31, 2020. The diagnosis of MAFLD was based on the diagnosis of fatty liver in the subject by ultrasound or computed tomography (CT) and the presence of one of the following conditions: overweight or obesity (body mass index [BMI] ⥠23), type 2 diabetes mellitus, and metabolic abnormalities. The subjects were divided into the GERS group (n = 305) and the non-GERS group (n = 2697) based on the presence or absence of GERS, based on the GerdQ score.Results: The prevalence of MAFLD was significantly higher in the GERS group than in the non-GERS group (p = 0.001). In the univariate analysis of risk factors for GERS, MAFLD was identified as a risk factor for GERS (OR 1.5; 95% CI 1.176â 1.913; p = 0.001). With adjustment of confounding factors such as BMI, waist circumference, lipid levels, and blood pressure, the correlation between MAFLD and GERS was attenuated but still significant (OR 1.408; 95% CI 1.085â 1.826; p = 0.010).Conclusion: MAFLD might be an independent risk factor for GERS.Keywords: gastroesophageal reflux symptoms, GERD, metabolic dysfunction-associated fatty liver disease, body mass index, obesity, questionnaire
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- 2021
9. Functional characterization of the horizontally transferred 4,5‐DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori
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Wei Sun, Cheng-Fang Wang, and Zhien Zhang
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0106 biological sciences ,0301 basic medicine ,Gene Transfer, Horizontal ,Transcription, Genetic ,Paralogous Gene ,Biology ,medicine.disease_cause ,01 natural sciences ,03 medical and health sciences ,Transcription (biology) ,Bombyx mori ,Dioxygenase ,Genetics ,medicine ,Animals ,Amino Acid Sequence ,Molecular Biology ,Gene ,Escherichia coli ,Phylogeny ,Synteny ,Base Sequence ,fungi ,Bombyx ,biology.organism_classification ,Dihydroxyphenylalanine ,010602 entomology ,030104 developmental biology ,Larva ,Insect Science ,Horizontal gene transfer ,Oxygenases ,Insect Proteins ,Sequence Alignment - Abstract
4,5-DOPA dioxygenase (DODA) is a crucial enzyme in the biosynthetic pathway of betalain. Previous studies have shown that DODA is present in plants, fungi and bacteria. Using updated data, here we show that DODA genes (BmDODA) in the domestic silkworm (Bombyx mori) and other lepidopteran insects are most likely to be horizontally transferred from fungi. A synteny analysis indicated that BmDODA1 is orthologous to other lepidopteran DODAs and that BmDODA2 is a paralogous gene. To explore the function of DODA in Lepidoptera, we first examined the expression patterns of BmDODA1. BmDODA1 showed high transcriptional and translational levels in the midgut and head. Then, we exogenously expressed the BmDODA1 gene, detected 4,5-DOPA ring-cleaving activity and calculated the kinetic parameters of the recombinant BmDODA1. We found that the transcription levels of BmDODA1 were significantly induced by the pathogens Bacillus bombyseptieus and Escherichia coli. Thus, the horizontal transfer of the BmDODA gene in the silkworm may be involved in dopa metabolism and contribute to antimicrobial activity in this species. Our results provide a documented example of functional horizontal gene transfer (HGT) between fungi and animals and expand our knowledge of HGT amongst eukaryotes.
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- 2019
10. A taxonomic revision of the genus Conidiobolus (Ancylistaceae, Entomophthorales): four clades including three new genera
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Cheng-Fang Wang, Xiao-Yong Liu, Bo Huang, Yong Nie, and Deshui Yu
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0106 biological sciences ,Paraphyly ,Morphology ,Biology ,010603 evolutionary biology ,01 natural sciences ,030308 mycology & parasitology ,Meteora ,03 medical and health sciences ,Monophyly ,New taxa ,Genus ,Molecular Systematics ,Polyphyly ,lcsh:Botany ,Unikonta ,Conidiobolus ,Clade ,Ribosomal DNA ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Taxonomy ,0303 health sciences ,Palavascia ,Entomophthoromycotina ,Ancylistaceae ,World ,Synchytriales ,Fungi ,Zygomycetes ,biology.organism_classification ,lcsh:QK1-989 ,Entomophthoromycetes ,Entomophthorales ,Taxon ,Evolutionary biology ,Zygomycetes Entomophthorales Morphology Phylogeny New taxa ,Zygomycota ,Research Article - Abstract
The genus Conidiobolus is an important group in entomophthoroid fungi and is considered to be polyphyletic in recent molecular phylogenies. To re-evaluate and delimit this genus, multi-locus phylogenetic analyses were performed using the large and small subunits of nuclear ribosomal DNA (nucLSU and nucSSU), the small subunit of the mitochondrial ribosomal DNA (mtSSU) and the translation elongation factor 1-alpha (EF-1α). The results indicated that the Conidiobolus is not monophyletic, being grouped into a paraphyletic grade with four clades. Consequently, the well-known Conidiobolus is revised and three new genera Capillidium, Microconidiobolus and Neoconidiobolus are proposed along with one new record and 22 new combinations. In addition, the genus Basidiobolus is found to be basal to the other entomophthoroid taxa and the genus Batkoa locates in the Entomophthoraceae clade.
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- 2020
11. Ecdysone oxidase and 3-dehydroecdysone-3β-reductase contribute to the synthesis of ecdysone during early embryonic development of the silkworm
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Ze Zhang, Wei Sun, and Cheng-Fang Wang
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0106 biological sciences ,0301 basic medicine ,Ecdysone ,3-Hydroxysteroid Dehydrogenases ,food.ingredient ,Ecdysterone ,Biology ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,chemistry.chemical_compound ,food ,Bombyx mori ,Yolk ,Gene expression ,Animals ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,fungi ,Embryogenesis ,Cell Biology ,Bombyx ,Prothoracic gland ,biology.organism_classification ,Cell biology ,010602 entomology ,030104 developmental biology ,chemistry ,Ecdysone oxidase ,Insect Proteins ,RNA Interference ,Developmental Biology - Abstract
Maternal ecdysteroids regulate a variety of cellular processes during early embryonic development of insects, yet little is known about the genes involved in the biosynthesis of these hormones. In this study, we found that ecdysone oxidase (EO) gene, which encodes an enzyme to catalyze ecdysone (or 20-hydroxyecdysone, 20E) to 3-dehydroecdysone (3DE), was highly expressed in the mature ovaries of the domestic silkworm, Bombyx mori. B. mori EO (BmEO) was localized in the cytoplasm around the yolk granules of oocyte. Furthermore, the down-regulated expression of the BmEO gene using RNA interference could not affect normal development of the female silkworm, but lower the 20E titer and hatching rate of its offspring. Rescue experiments by injecting the product (3DE) of BmEO can significantly elevate the 20E level and hatching rate of the BmEO RNAi offspring. Meanwhile, during embryonic stage, the down-regulating expression of 3DE-3β-reductase, which can reduce 3DE into ecdysone, also lowered the 20E titer. Taken together, our results prove that 3DE can be synthesized from ecdysone in maternal ovary yolk granules, and then the maternal 3DE is converted into active ecdysone during the early embryonic development of offspring. Thus, our findings reveal a new pathway to explain the origin of high 20E level before the formation the prothoracic gland in the silkworm.
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- 2018
12. Nomograms Predicting Response and Survival of Esophageal Squamous Cell Carcinoma Patients Treated With Anti-PD-1 Therapy
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Jie Chen, Cheng-Fang Wang, S. Nie, S. Zhu, Xuejiao Hu, and Yongyan Wang
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Oncology ,Cancer Research ,medicine.medical_specialty ,Radiation ,business.industry ,Proportional hazards model ,Lymphocyte ,Albumin ,Nomogram ,Logistic regression ,Esophageal squamous cell carcinoma ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Internal medicine ,Lactate dehydrogenase ,Absolute neutrophil count ,Medicine ,Radiology, Nuclear Medicine and imaging ,business - Abstract
Purpose/Objective(s) In recent years, anti-programmed death-1 (PD-1) antibody has played an increasingly critical role in the treatment of patients with esophageal squamous cell carcinoma (ESCC), but there is still a lack of credible biomarkers to predict the efficacy of anti-PD-1 therapy. Our study aimed to develop a nomogram to predict the response and survival of patients with ECSS treated with anti-PD-1. Materials/Methods A total of 236 patients who received anti-PD-1 therapy between January 2019 and September 2020 were collected in the study retrospectively. Absolute neutrophil count (ANC), absolute lymphocyte count (ALC), total lymphocyte count, platelet count (PLT), monocyte count, lactate dehydrogenase (LDH), and serum albumin level at baseline were used to calculate the NLR (ANC/ALC), PLR (PLT/ALC), LMR (ALC/monocyte count), and PNI (10×serum albumin value, g/dL+0.005×total lymphocyte count/mm3). The early response was assessed by the RECIST version 1.1 criteria. Logistic and Cox models were used to identify factors associated with early response and survival, respectively. Nomograms were developed based on significant factors and evaluated by Concordance-index (C-index) and calibration curve. The 1,000 bootstrap samples and calibration curves were used to validate the discrimination of the nomogram. Results In multivariate logistic regression analysis, NLR Conclusion The nomogram established by combining the clinical characteristics and peripheral blood markers of patients could effectively predict the prognosis of patients with ESCC treated with anti-PD-1 therapy.
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- 2021
13. Essential oil and polyacetylenes from Artemisia ordosica and their bioactivities against Tribolium castaneum Herbst (Coleoptera: Tenebrionidae)
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Wen-Juan Zhang, Zhe Zhang, Zhi-Wei Deng, Zhu-Feng Geng, Shu-Shan Du, Junyu Liang, Cheng-Fang Wang, and Shan-Shan Guo
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0106 biological sciences ,010405 organic chemistry ,Chemistry ,Principal factor ,01 natural sciences ,0104 chemical sciences ,law.invention ,DEET ,chemistry.chemical_compound ,Artemisia ordosica ,Capillinol ,law ,Botany ,Bioassay ,Food science ,Agronomy and Crop Science ,Essential oil ,010606 plant biology & botany - Abstract
Five polyacetylenes (1-5) were isolated from Artemisia ordosica, one of the main arido-active shrubs growing in the arid areas in China. Their molecular structures were identified with NMR spectroscopy. The compounds were confirmed as capillene, capillin, capillinol, cis-dehydromatricaria ester and trans-dehydromatricaria ester. It was found that three compounds (1-3) had similiar structural skeleton of 2,4-hexadiyn-1-ylbenzene. The repellent and fumigant activities against Tribolium castaneum adults were investigated for compounds isolated and the essential oil. Bioassays showed that the essential oil extracted from the aerial parts of A. ordosica possessed fumigant toxicity of LC50 = 18.65 mg/L air, and compounds (1-4) had stronger activities from LC50 = 4.06 mg/L air to 6.16 mg/L air against T. castaneum adults. At the tested concentrations (62.91 and 12.58 nL/cm2), the crude essential oil and compounds (1, 2 and 4) showed the same repellent activity (p > 0.05) with DEET against T. castaneum adults at both 2 h and 4 h after exposure. Compound 2 showed strong repellence (PR = 100%) at tested concentrations of 62.91, 12.58 and 2.52 nL/cm2 at 2 h after exposure. Moreover, at tested concentration (62.91, 12.58, 2.52 and 0.5 nL/cm2), the PR value of compound 4 was all higher than 90% at both 2 h and 4 h after exposure. The results showed that the crude essential oil and the isolated polyacetylenes possessed fair repellent and fumigant activities. Furthermore, potential relationships between structure and bioactivity were also discussed. It might be found that the existence of acetylenic bonds was the principal factor of fumigant activity, and with the increase of acetylenic bonds, the fumigant activity would enhance. In addition, it was believed that oxygenous groups connected to the acetylenic bonds might contribute to fumigant toxicity against T. castaneum adults.
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- 2017
14. Transcription factor E74A affects the ecdysone titer by regulating the expression of the EO gene in the silkworm, Bomby mori
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Ze Zhang, Wei Sun, and Cheng-Fang Wang
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0301 basic medicine ,Ecdysone ,3-Hydroxysteroid Dehydrogenases ,Transcription, Genetic ,Biophysics ,Down-Regulation ,Regulatory Sequences, Nucleic Acid ,Biology ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,RNA interference ,Animals ,Hormone metabolism ,Molecular Biology ,Transcription factor ,Bombyx ,Prothoracic gland ,Molecular biology ,Chromatin ,Hormones ,Cell biology ,030104 developmental biology ,chemistry ,Ecdysone oxidase ,Insect Proteins ,Ecdysone receptor ,Chromatin immunoprecipitation ,030217 neurology & neurosurgery ,Transcription Factors - Abstract
The formation of ecdysone pulse in insects is synergistically controlled by its biosynthesis and degradation. Previous studies have revealed the feedback regulation of the prothoracic gland (PG) activity to affect the hormone synthesis. However, the molecular regulatory mechanism of the ecdysone degradation is still unclear. In this study, we showed that ecdysone oxidase (EO) gene encoding a hormone metabolism enzyme was also induced by hormone itself in the domestic silkworm, Bombyx mori. Furthermore, luciferase reporter, chromatin immunoprecipitation and electrophoretic mobility shift assays showed that ecdysone inducible transcription factor E74A could bind to the cis-regulatory elements of the EO gene. Then, down-regulating the expression of the E74A by RNA interference (RNAi) decreased the expression of the EO gene and caused a higher ecdysone titer compared with the control. Thus, our results demonstrated a new feedback regulation degradation (EO) pathway controlled by ecdysone itself through transcription factor E74A, expanding the knowledge about the regulatory system that determines the formation of ecdysone pulse.
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- 2017
15. Research on Problem of Urban Regeneration and Optimization of Land-use around Light Rail Transit in Nanhai District of Foshan City
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Ying Xu, Lu Xiong, Jian-wen Huang, Jia Hai, and Cheng-fang Wang
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Geographic information system ,Land use ,business.industry ,0211 other engineering and technologies ,021107 urban & regional planning ,Urban regeneration ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Transport engineering ,Geography ,Light rail transit ,Public transport ,business ,0105 earth and related environmental sciences - Abstract
Nanhai district of Foshan city is the key regeneration area to realize the strategy of ‘Guangzhou and Foshan to be the one’. And the project of Light Rail Transit (LRT) system launched recently in Nanhai district will not only improve the levels of Public Transportation System (PTS), but also strengthen the connection of PTS between Guangzhou and Foshan. This paper made use of Geographic Information System (GIS) platform and multiple tools on spatial analysis to conduct a multi-dimensional empirical study for Areas around several stations of Nanhai LRT. By exploring optimization strategy of surrounding land-use from a multi- disciplinary overlapping aspect, the study provides some advices for the future construction of LRT in Foshan city and surrounding land-use optimizing control.
- Published
- 2017
16. Repellent activity of compounds from Murraya alata Drake against Tribolium castaneum
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Zhe Zhang, Junyu Liang, Cheng-Fang Wang, Wen-Juan Zhang, Chun-Xue You, Zhu-Feng Geng, Shan-Shan Guo, Shu-Shan Du, and Zhi-Wei Deng
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Traditional medicine ,010405 organic chemistry ,Positive control ,Murraya alata ,Phebalosin ,Biology ,01 natural sciences ,0104 chemical sciences ,DEET ,Toxicology ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Statistical analysis ,Spectral analysis ,Agronomy and Crop Science ,Complex problems - Abstract
Murraya alata Drake is a shrub that is distributed in the underwood of sandy areas near sea level in China and Vietnam. Twenty-two compounds were isolated from the leaves and stems of M. alata, including six new coumarins (6, 8–10, 17, 19) named muralatins L–Q. The structures were identified on the base of NMR and MS spectral analysis. Furthermore, the repellent activity against Tribolium castaneum was tested for fourteen isolated compounds. A statistic model-grey relational analysis was introduced to evaluate repellent activity directly based on relative correlation degree. This statistical analysis was suitable for complex problems of multiple indexes. The testing compounds exhibited various repellent activities against T. castaneum. Among them, compounds 1 (meranzin), 2 (phebalosin) and 16 (muralatin K) showed significant repellent activity against T. castaneum with ri values of 0.7047, 0.6990 and 0.6884, respectively, comparable to that of the positive control, N, N-diethyl-3-methylbenzamide (DEET, ri = 0.7013). The optimal compounds might be considered as potential leading compounds for the development of natural repellents.
- Published
- 2017
17. Contact and Repellant Activities of Zerumbone and Its Analogues from the Essential Oil of Zingiber zerumbet (L.) Smith against Lasioderma serricorne
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Tian Zhaofu, Wang Pingjuan, Huang Dongye, Shan-Shan Guo, Shu-Shan Du, Li Zhihua, Wu Yan, Cheng-Fang Wang, Wei Jianyu, Sun Jiansheng, and Bai Jiafeng
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0106 biological sciences ,biology ,Humulene ,Traditional medicine ,General Chemical Engineering ,General Medicine ,General Chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Rhizome ,law.invention ,010602 entomology ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Zingiber zerumbet ,chemistry ,law ,Toxicity ,Botany ,Lasioderma serricorne ,Camphene ,Zingiberaceae ,Essential oil - Abstract
The contact toxicity and repellent activities of the essential oil extracted from the rhizomes of Zingiber zerumbet (L.) Smith (Zingiberaceae) was evaluated against cigarette beetles (Lasioderma serricorne). The essential oil obtained by hydrodistillation was investigated by GC-FID and GC-MS. The main constituents of the essential oil were zerumbone (40.2%), α-caryophyllene (8.6%), humulene epoxide II (7.3%), camphene (5.9%) and fenchene (4.7%). Zerumbone and its analogues totally are accounting for 60.3% of the essential oil. It was found that the essential oil possessed contact toxicity against L. serricorne adults with a LD50 value of 48.3 µg/adult. α-Caryophyllene (LD50 = 13.1 µg/adult) exhibited stronger contact toxicity against L. serricorne than humulene oxide (LD50 = 31.2 µg/adult), β-caryophyllene (LD50 = 35.5 µg/adult) and zerumbone (LD50 = 42.4 µg/adult). Moreover, α-caryophyllene possessed strong repellent activity (Class IV and V, respectively) against the beetles at 78.63 nL/cm2, after 2 and 4 h treatment. The results indicate that zerumbone and its analogues might be developed into natural insecticides or repellents for control of cigarette beetles, but their bioactivities are affected by their structures.
- Published
- 2017
18. Repellence of the main components from the essential oil of Glycosmis lucida Wall. ex Huang against two stored product insects
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Yin-Ping Li, Chun-Xue You, Wen-Juan Zhang, Shu-Shan Du, Kai Yang, Shan-Shan Guo, Junyu Liang, Zhu-Feng Geng, Cheng-Fang Wang, and Zhi-Wei Deng
- Subjects
Liposcelis bostrychophila ,Allylbenzene Derivatives ,Plant Science ,Anisoles ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Spathulenol ,law.invention ,Toxicology ,chemistry.chemical_compound ,law ,Oils, Volatile ,Animals ,Rutaceae ,Verbenone ,Anethole ,Essential oil ,Tribolium ,biology ,Traditional medicine ,010405 organic chemistry ,Glycosmis lucida ,Organic Chemistry ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Insect Repellents ,Medicinal herbs - Abstract
A screening of Chinese medicinal herbs and wild plants for agrochemicals was carried out; the essential oil of Glycosmis lucida leaves was found to possess significant repellent activity against Tribolium castaneum and Liposcelis bostrychophila. It was found that the main components included elixene (19.81%), spathulenol (10.68%), anethole (12.05%), verbenone (10.32%) followed by β-caryophyllene (6.87%). The essential oil, anethole and verbenone were strongly repellent against T. castaneum (96, 86 and 94%, respectively, at 15.73 nL cm−2) and L. bostrychophila (100, 68 and 72%, respectively, at 31.58 nL cm−2) after a 2h treatment. The results indicate that anethole and verbenone had the potential to be developed as natural repellents for control of stored product insects.
- Published
- 2016
19. Chemical Constituents and Insecticidal Activities of Ajania fruticulosa Essential Oil
- Author
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Shan Shan Guo, Wen-Juan Zhang, Zhu-Feng Geng, Ji Zhang, Chun-Xue You, Shu-Shan Du, Zhi-Wei Deng, Jun Yu Liang, and Cheng-Fang Wang
- Subjects
Insecticides ,Insecta ,Plant composition ,Bioengineering ,Asteraceae ,01 natural sciences ,Biochemistry ,law.invention ,Camphor ,chemistry.chemical_compound ,law ,Myrtenol ,Oils, Volatile ,Animals ,Food science ,Molecular Biology ,Chemical composition ,Essential oil ,Dose-Response Relationship, Drug ,010405 organic chemistry ,General Chemistry ,General Medicine ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,chemistry ,Chemical constituents ,Toxicity ,Molecular Medicine ,Ajania fruticulosa - Abstract
The insecticidal activity and chemical constituents of the essential oil from Ajania fruticulosa were investigated. Twelve constituents representing 91.0% of the essential oil were identified, and the main constituents were 1,8-cineole (41.40%), (+)-camphor (32.10%), and myrtenol (8.15%). The essential oil exhibited contact toxicity against Tribolium castaneum and Liposcelis bostrychophila adults with LD50 values of 105.67 μg/adult and 89.85 μg/cm(2) , respectively. The essential oil also showed fumigant toxicity against two species of insect with LC50 values of 11.52 and 0.65 mg/l, respectively. 1,8-Cineole exhibited excellent fumigant toxicity (LC50 = 5.47 mg/l) against T. castaneum. (+)-Camphor showed obvious fumigant toxicity (LC50 = 0.43 mg/l) against L. bostrychophila. Myrtenol showed contact toxicity (LD50 = 29.40 μg/cm(2) ) and fumigant toxicity (LC50 = 0.50 mg/l) against L. bostrychophila. 1,8-Cineole and (+)-camphor showed strong insecticidal activity to some important insects, and they are main constituents of A. fruticulosa essential oil. The two compounds may be related to insecticidal activity of A. fruticulosa essential oil against T. castaneum and L. bostrychophila.
- Published
- 2016
20. Chemical Composition of Essential Oils from Zanthoxylum bungeanum Maxim. and Their Bioactivities against Lasioderma serricorne
- Author
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Chun-Xue You, Junyu Liang, Zhu-Feng Geng, Shu-Shan Du, Zhi-Wei Deng, Wen-Juan Zhang, Cheng-Fang Wang, Yong-Yan Wang, and Shan-Shan Guo
- Subjects
0106 biological sciences ,Limonene ,biology ,010405 organic chemistry ,General Chemical Engineering ,Nonanoic acid ,General Medicine ,General Chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,law.invention ,010602 entomology ,chemistry.chemical_compound ,Eucalyptol ,Linalool ,chemistry ,Zanthoxylum ,law ,Botany ,Lasioderma serricorne ,Food science ,Gas chromatography–mass spectrometry ,Essential oil - Abstract
Two essential oil samples were obtained from the pericarp of Zanthoxylum bungeanum with the methods of hydrodistillation (HD) and supercritical fluid CO2 extraction (SFE), their chemical components were identified, and their bioactivities against Lasioderma serricorne adults were evaluated. In the process of testing, the two samples showed significant bioactivities against Lasioderma serricorne adults. For an example, the SFE-sample expressed relatively stronger fumigant toxicity on Lasioderma serricorne adults (LC50 = 3.99 μg/mL air) than that of the HD-sample (LC50 = 12.54 μg/mL air). According to GC-MS analysis, the chemical components between two samples were different. The major chemical components for HD included linalool (25.99%), limonene (19.34%), linalyl anthranilate (12.22%), 4-terpinenol (10.49%), eucalyptol (6.53%) and α-terpineol (5.02%), while for SFE included nonanoic acid (21.43%), γ-terpinene (14.51%), eucalyptol (13.45%), α-terpineol (5.83%) and caryophyllene oxide (5.48%). The results showed that different chemical components result in different bioactivities. This work provides theoretical basis for traditional Chinese concept of antagonistic storage, and it also provides important information for the development and comprehensive utilization of Zanthoxylum bungeanum.
- Published
- 2016
21. Chemical constituents of the essential oil extracted from Rhododendron thymifolium and their insecticidal activities against Liposcelis bostrychophila or Tribolium castaneum
- Author
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Yuan Li, Zhi-Wei Deng, Shan-Shan Guo, Shu-Shan Du, Cheng-Fang Wang, Wen-Juan Zhang, Chun-Xue You, Zhu-Feng Geng, Ji Zhang, and Junyu Liang
- Subjects
0106 biological sciences ,010405 organic chemistry ,Liposcelis bostrychophila ,Positive control ,Germacrone ,Biology ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,law.invention ,Toxicology ,010602 entomology ,chemistry.chemical_compound ,chemistry ,law ,Chemical constituents ,Toxicity ,Agronomy and Crop Science ,Essential oil - Abstract
In this work, we investigated the chemical constituents of the essential oil extracted from the leaves of Rhododendron thymifolium and their insecticidal and repellent activities against Liposcelis bostrychophila and Tribolium castaneum. 14 chemical components were identified from the essential oil and included germacrone (20.83%), γ-elemene (11.10%), selina-3,7(11)-diene (6.18%), α-oxobisabolene (5.39%), β-sesquiphellandrene (2.44%), β-elemenone (35.17%), Z,Z,Z-1,5,9,9-tetramethyl-1,4,7,-cycloundecatriene (3.64%) and 1-(4-ethylphenyl)-3-methyl-pyrazol-(4H)-one (2.91%). Among them, the first five components were isolated and identified. The results showed that the essential oil and five pure compounds possessed the obvious repellent activity and various levels of contact activities against the two species of stored insects. The essential oil possessed the contact toxicity (LD50 = 19.63 μg/cm2) which was quite close to the toxicity of positive control pyrethrins (LD50 = 18.72 μg/cm2) against L. bostrychophila. The five pure compounds might individually or together effect the contact toxicity of the essential oil against L. bostrychophila. The essential oil possessed the contact toxicity (LD50 = 29.82 μg/adult) against T. castaneum, but only germacrone revealed strong contact toxicity (LD50 = 0.97 μg/adult), which was comparable with the toxicity of pyrethrins (LD50 = 0.24 μg/adult) among five compounds against T. castaneum. The existence of germacrone might be one of the key elements which affected the contact toxicity of R. thymifolium essential oil against T. castaneum. The insecticidal and repellent activities of essential oil and five compounds were evaluated, the results provide a basis of the development and utilization in the future for control of insects.
- Published
- 2016
22. Chemical composition of essential oils extracted from six Murraya species and their repellent activity against Tribolium castaneum
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Ying Wang, Zhi-Wei Deng, Zhu-Feng Geng, Shan-Shan Guo, Junyu Liang, Shu-Shan Du, Chun-Xue You, Cheng-Fang Wang, Wen-Juan Zhang, and Kai Yang
- Subjects
Murraya euchrestifolia ,Traditional medicine ,Murraya ,biology ,biology.organism_classification ,Bioactive compound ,chemistry.chemical_compound ,chemistry ,Caryophyllene oxide ,Murraya tetramera ,Botany ,Camphene ,Murraya kwangsiensis ,Agronomy and Crop Science ,Chemical composition - Abstract
Murraya genus (family Rutaceae) has been widely used as local traditional medicines in East Asia. The chemical composition of essential oils extracted from six Murraya species and their repellent activity against Tribolium castaneum was investigated. From the samples of Murraya tetramera , Murraya euchrestifolia , Murraya koenigii , Murraya kwangsiensis , Murraya exotica , and Murraya alata , essential oils were obtained by hydrodistillation and their yields were various from 0.03 to 0.23%. All together 36 components were identified by GC–MS from the essential oils samples. The constituents with relatively higher contents included α-cedrene (4.22–21.82%), β-caryophyllene (8.15–27.73%), γ-elemene (4.38–12.65%), γ-selinene (3.80–21.09%), α-selinene (2.60–10.68%), α-zingiberene (2.08–12.68%), spathulenol (3.08–11.80%), caryophyllene oxide (1.58–10.59%), β-eudesmol (1.33–25.32%), etc. Moreover, eight compounds, which were recognized as significant components in the essential oils of M. tetramera , and M. kwangsiensis , were isolated and they were identified as α-terpinene, β-caryophyllene, α-caryophyllene, alloaromadendrene, spathulenol, β-eudesmol, camphene and caryophyllene oxide. The repellent activity against T. castaneum was tested for all the six samples of essential oils and eight isolated compounds. The testing samples exhibited a variety of repellent activity against T. castaneum . Among them, the essential oils of two species ( M. tetramera and M. kwangsiensis ) and three isolated compounds (alloaromadendrene, spathulenol and caryophyllene oxide) showed the fair repellent activity with relatively higher yields of contents. They might be considered as potential materials for the development of natural repellents for control of insects in stored products. Meanwhile, cluster analysis was performed using the collectives of data which were obtained in the process of the experiments. It was found that the characteristics of the repellent activity against T. castaneum could be related to the chemical composition of the essential oils. There were some interesting senses brought out as well. Such as, the repellent activity of the essential oils would not be depend on the contents of individual bioactive compound, and the existence of some compounds might affect the repellent activity of the essential oils.
- Published
- 2015
23. Antifeedant activities of methanol extracts of four Zanthoxylum species and benzophenanthridines from stem bark of Zanthoxylum schinifolium against Tribolium castaneum
- Author
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Zhu-Feng Geng, Cheng-Fang Wang, Zhi-Wei Deng, Chun-Xue You, Shu-Shan Du, Li Fan, Shan-Shan Guo, Kai Yang, and Yong-Yan Wang
- Subjects
Norchelerythrine ,Stem bark ,biology ,Chemistry ,Benzophenanthridines ,biology.organism_classification ,Methylenedioxy ,chemistry.chemical_compound ,Zanthoxylum ,visual_art ,Botany ,visual_art.visual_art_medium ,Bark ,Methanol ,Agronomy and Crop Science ,Zanthoxylum schinifolium - Abstract
Antifeedant activities of the methanol extracts from Zanthoxylum bungeanum , Zanthoxylum schinifolium , Zanthoxylum armatum and Zanthoxylum dissitum were assessed on Tribolium castaneum adults. It was found that the methanol extract of stem bark of Z. schinifolium had the highest antifeedant activity at 41.12% (antifeedant index). Based on bioactivity-guided fractionation, six benzophenanthridines norchelerythrine ( 1 ), decarine ( 2 ), 8-hydroxy-9-methoxy-2,3-(methylenedioxy) benzophenanthridine ( 3 ), 6-hydroxydihydrochelerythrine ( 4 ), 6-methoxy-7-hydroxydihydrochelerythrine ( 5 ) and oxychelerythrine ( 6 ) were isolated from the stem barks of Z. schinifolium . And their antifeeding activities were also evaluated against T. castaneum . All of them exhibited strong antifeeding activity in a concentration-dependant manner with EC 50 of 62.67, 66.97, 151.39, 96.72, 141.61 and 192.32 ppm, respectively. The six bioactive compounds from the stem bark of Z. schinifolium might be used as antifeedants against T. castaneum .
- Published
- 2015
24. Five new sulphur-containing amides from Glycosmis lucida with antifeedant activity against Tribolium castaneum
- Author
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Zhi-Wei Deng, Wen-Juan Zhang, Zhu-Feng Geng, Chun-Xue You, Shu-Shan Du, Yin-Ping Li, Shan-Shan Guo, Cheng-Fang Wang, and Kai Yang
- Subjects
chemistry.chemical_compound ,chemistry ,Stereochemistry ,Acrylamide ,Glycosmis lucida ,chemistry.chemical_element ,Nuclear magnetic resonance spectroscopy ,Biology ,Agronomy and Crop Science ,Sulfur - Abstract
Five new sulphur-containing amides (2, 5–8) and three known analogue compounds (1, 3–4) were isolated from the leaves of Glycosmis lucida which were collected in Yunnan province, China. Their structures were elucidated with NMR spectroscopy and mass spectrometry. The compounds were identified as N-(p-hydroxyphenethyl)-3-(methylsulfonyl)-propenamide (1), N-[2-(4- hydroxyphenyl)-ethyl]-3-methanesulfonyl-N-methyl-propionamide (2), methylgerambullin (3), methylisogerambullone (4), methylgerambullindiol (5), N-{2-[4-(6,7-hydroxy-3,7-dimethyl-oct- 2-enyloxy)-phenyl]-ethyl}-3-methanesulfonyl-N-methyl-acrylamide (6), N-{2-[4-(6,7-dihydroxy- 3,7-dimethyl-oct-2-enyloxy)-phenyl]-ethyl}-3-methanesulfonyl-N-methyl-propionamide (7), (E)-N-{2-[4-(6-hydroxy-7-dimethyl-oct-2-enyloxy)-phenyl]-ethyl}-3-methanesulfinyl-N-methyl- acrylamide (8). Feeding deterrent activities of these surphur-containing derivatives against Tribolium castaneum were assayed for the first time. Compound 2, 3, 6 possessed significant feeding deterrent activities against T. castaneum adults (EC50 = 311.6, 244.9 and 108.3 ppm, respectively). These results demonstrated that some sulphur-containing amides are promising candidates as insect antifeedant agents.
- Published
- 2015
25. Contact Toxicity and Repellency of the Essential Oil fromMentha haplocalyx<scp>Briq</scp>. againstLasioderma serricorne
- Author
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Shu-Shan Du, Yang Su, Cheng-Fang Wang, Zhi-Wei Deng, Wen-Juan Zhang, Kai Yang, Chun-Xue You, Ying Wang, and Zhu-Feng Geng
- Subjects
Insecticides ,Bioengineering ,Fractionation ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,law.invention ,DEET ,chemistry.chemical_compound ,law ,Oils, Volatile ,Animals ,Plant Oils ,Organic chemistry ,Food science ,Molecular Biology ,Essential oil ,Limonene ,Dose-Response Relationship, Drug ,biology ,Chemistry ,General Chemistry ,General Medicine ,biology.organism_classification ,Menthone ,Coleoptera ,Insect Repellents ,Lasioderma serricorne ,Molecular Medicine ,Menthyl acetate ,Menthol ,Mentha - Abstract
The chemical composition of the essential oil obtained by hydrodistillation from the aerial parts of Mentha haplocalyx was investigated by GC-FID and GC/MS analyses. In sum, 23 components, representing 92.88% of the total oil composition, were identified, and the main compounds were found to be menthol (59.71%), menthyl acetate (7.83%), limonene (6.98%), and menthone (4.44%). By bioassay-guided fractionation (contact toxicity), three compounds were obtained from the essential oil and identified as menthol, menthyl acetate, and limonene. The essential oil and the three isolated compounds exhibited potent contact toxicity against Lasioderma serricorne adults, with LD50 values of 16.5, 7.91, 5.96, and 13.7 μg/adult, respectively. Moreover, the oil and its isolated compounds also exhibited strong repellency against L. serricorne adults. At the lower concentrations tested and at 2 h after exposure, menthol showed even significantly stronger repellency than the positive control DEET. The study revealed that the bioactivity properties of the essential oil can be attributed to the synergistic effects of its diverse major and minor components, which indicates that the M. haplocalyx oil and its isolated compounds have potential for the development as natural insecticides and/or repellents to control insects in stored grains and traditional Chinese medicinal materials.
- Published
- 2015
26. Cytotoxicity of benzophenanthridine alkaloids from the roots ofZanthoxylum nitidum(Roxb.) DC. var.fastuosumHow ex Huang
- Author
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Zhi-Wei Deng, Xu Su, Mei Tian, Li Fan, Shu-Shan Du, Yong-Yan Wang, Cheng-Fang Wang, and Jiang-Bin Feng
- Subjects
Benzophenanthridines ,Zanthoxylum ,Molecular Structure ,biology ,Traditional medicine ,Chemistry ,Organic Chemistry ,Plant Science ,biology.organism_classification ,Plant Roots ,Biochemistry ,In vitro ,Analytical Chemistry ,Zanthoxylum nitidum ,HeLa ,Alkaloids ,Cell culture ,Cell Line, Tumor ,Humans ,Potency ,Medicinal plants ,Cytotoxicity - Abstract
This work aimed to investigate benzophenanthridine from the roots of Zanthoxylum nitidum (Roxb.) DC. var. fastuosum How ex Huang for the first time. Thirteen benzophenanthridines were isolated, and our results of the cytotoxic activities indicated that compound 6 exhibited the best potency against A549, Hela, SMMC-7721 and EJ, with the IC50 values of 27.50, 37.50, 16.95 and 60.42 μM, respectively. Compounds 7 and 11 also showed strong cytotoxicity when tested against the four human cancer cell lines (A549, Hela, SMMC-7721 and EJ), while only compounds 12 and 13 displayed cytotoxicity in inhibiting BALL-1 proliferation among all the compounds. These results suggested that benzophenanthridines may become a valid alternative of potential basis for new anti-proliferative agents.
- Published
- 2015
27. Chemical Composition and Bioactivity of Essential Oil of Atalantia guillauminii against Three Species Stored Product Insects
- Author
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Zhu-Feng Geng, Zhi-Wei Deng, Ping Ma, Shan-Shan Guo, Shu-Shan Du, Kai Yang, Ning Lei, Cheng-Fang Wang, and Chun-Xue You
- Subjects
biology ,General Chemical Engineering ,Liposcelis bostrychophila ,Elemicin ,General Medicine ,General Chemistry ,biology.organism_classification ,Median lethal dose ,law.invention ,DEET ,chemistry.chemical_compound ,Rutaceae ,chemistry ,law ,Toxicity ,Lasioderma serricorne ,Food science ,Essential oil - Abstract
The toxic and repellent activities of the essential oil extracted from the leaves of Atalantia guillauminii Swingle were evaluated against three stored product insects, red flour beetles (Tribolium castaneum), cigarette beetles (Lasioderma serricorne) and booklice (Liposcelis bostrychophila). The essential oil obtained by hydrodistillation was investigated by GC-MS. The main constituents of the essential oil were β-thujene (27.18%), elemicin (15.03%), eudesma-3, 7(11)-diene (9.64%), followed by (-)-4-terpeniol (6.70%) and spathulenol (5.25%). The crude oil showed remarkable contact toxicity against T. castaneum, L. serricorne adults and L. bostrychophila with LD50 values of 17.11, 24.07 µg/adult and 55.83 µg/cm(2) respectively and it also displayed strong fumigant toxicity against T. castaneum, L. serricorne adults with LC50 values of 17.60 and 12.06 mg/L respectively, while weak fumigant toxicity against L. bostrychophila with a LC50 value of 16.75 mg/L. Moreover, the essential oil also exhibited the same level repellency against the three stored product insects, relative to the positive control, DEET. At the same concentrations, the essential oil was more repellent to T. castaneum than to L. serricorne. Thus, the essential oil of A. guillauminii may be potential to be developed as a new natural fumigant/repellent in the control of stored product insects.
- Published
- 2015
28. Insecticidal Constituents of Essential Oil Derived from Zanthoxylum armatum against Two Stored-Product Insects
- Author
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Zhi-Wei Deng, Shu-Shan Du, Chun-Xue You, Wen-Juan Zhang, Zhu-Feng Geng, Cheng-Fang Wang, Yong-Yan Wang, Li Fan, and Shan-Shan Guo
- Subjects
Limonene ,biology ,General Chemical Engineering ,General Medicine ,General Chemistry ,biology.organism_classification ,law.invention ,Toxicology ,chemistry.chemical_compound ,Rutaceae ,chemistry ,law ,Toxicity ,Lasioderma serricorne ,Bioassay ,Food science ,Medicinal plants ,Essential oil ,Piperitone - Abstract
In the course of our search for natural bioactive chemicals and investigations on their insecticidal activities from some medicinal plants growing in China, the essential oil derived from the twigs of Zanthoxylum armatum (Rutaceae) was found to possess strong insecticidal activities against two stored-product insects, Lasioderma serricorne and Tribolium castaneum. A total of 32 constituents of the essential oil were identified by GC and GC-MS analysis, and it revealed (E)-anethole (20.5%), 1,8-cineole (14.0%), 2-tridecanone (12.5%), limonene (9.0%) and piperitone (8.0%) as major components, followed by β-phellandrene (6.3%), β-pinene (5.1%) and 4-terpineol (4.4%). From the essential oil, five compounds were isolated and identified as (E)-anethole, 1,8-cineole, 2-tridecanone, limonene and piperitone. The results of insecticidal bioassays showed that the essential oil of Z. armatum exhibited strong fumigant toxicity towards L. serricorne and T. castaneum with LC50 values of 13.83 and 4.28 mg/L air, respectively, and also possessed contact toxicity against two insect species with LD50 values of 18.74 and 32.16 μg/adult, respectively. Among the active compounds, piperitone performed the strongest fumigant toxicity against L. serricorne (LC50 = 1.21 mg/L air) and contact toxicity against T. castaneum (LD50 = 3.16 μg/adult). 1,8-Cineole, limonene and piperitone showed similar fumigant toxicity against T. castaneum with LC50 values of 5.47, 6.21 and 7.12 mg/L air, respectively. Meanwhile, L. serricorne was the most sensitive to 2-tridecanone (LD50 = 5.74 μg/adult) in the progress of contact toxicity assay.
- Published
- 2015
29. Bioactivity and Chemical Constituents of the Essential Oil from Dendranthema indicum (L.) Des Moul. against Two Stored Insects
- Author
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Kai Yang, Wen-Juan Zhang, Yong-Yan Wang, Cheng-Fang Wang, Zhu-Feng Geng, Chun-Xue You, Shu-Shan Du, Zhi-Wei Deng, Ying Wang, and Yang Su
- Subjects
business.industry ,General Chemical Engineering ,Chamazulene ,Food storage ,General Medicine ,General Chemistry ,Biology ,law.invention ,Biotechnology ,chemistry.chemical_compound ,Horticulture ,Eucalyptol ,chemistry ,law ,Chemical constituents ,Medicinal herbs ,Gas chromatography–mass spectrometry ,business ,Essential oil ,Germacrene D - Abstract
Dendranthema indicum (L.) Des Moul. is a perennial herb commonly used as a traditional Chinese medicine for a long time. In this work, we took Dendranthema indicum as a target plant and two stored insects which include Tribolium castaneum and Stegobium paniceum adults as target insects. Essential oil obtained from Dendranthema indicum was analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 31 components representing 92.44% of the oil were identified and the main compounds were found to be chamazulene (15.93%), β-caryophyllene (13.78%), germacrene D (9.11%), and b-cis-farnesene (6.59%). With a further isolation, three constituents were obtained from the essential oil and identified as chamazulene, β-caryophyllene and eucalyptol. Significantly, in the progress of assay, it showed that the essential oil and chamazulene exhibited stronger insecticidal and repellent activities against Stegobium paniceum than Tribolium castaneum. The results indicate that additional to its traditional use as Chinese medicinal herb, the essential oil of Dendranthema indicum aerial parts and isolated compounds have potential to be developed into natural insecticides or repellents for control of insects in stored grains.
- Published
- 2015
30. Essential Oil of Amomum maximum Roxb. and Its Bioactivities against Two Stored-Product Insects
- Author
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Shan-Shan Guo, Chun-Xue You, Wen-Juan Zhang, Junyu Liang, Ning Lei, Shu-Shan Du, Kai Yang, Zhu-Feng Geng, and Cheng-Fang Wang
- Subjects
Chromatography ,South china ,biology ,Chemistry ,General Chemical Engineering ,Liposcelis bostrychophila ,Fumigation ,General Medicine ,General Chemistry ,biology.organism_classification ,Amomum ,law.invention ,Southeast asia ,Horticulture ,law ,Amomum maximum ,Gas chromatography–mass spectrometry ,Essential oil - Abstract
Amomum maximum Roxb. is a perennial herb distributed in South China and Southeast Asia. The objective of this work was to analyze the chemical constituents and assess insecticidal and repellent activities of the essential oil from Amomum maximum fruits against Tribolium castaneum (Herbst) and Liposcelis bostrychophila (Badonnel). The essential oil was obtained by hydrodistillation and analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. The main components of the essential oil were identified to be β-pinene (23.39%), β-caryophyllene (16.43%), α-pinene (7.55%), sylvestrene (6.61%) and c-cadinene (4.19%). It was found that the essential oil of A. maximum fruits possessed contact and fumigant toxicities against T. castaneum adults (LD50 = 29.57 μg/adult and LC(50) = 23.09 mg/L air, respectively) and showed contact toxicity against L. bostrychophila (LD(50) = 67.46 μg/cm(2)). Repellency of the crude oil was also evaluated. After 2 h treatment, the essential oil possessed 100% repellency at 78.63 nL/cm(2) against T. castaneum and 84% repellency at 63.17 nL/cm(2) against L. bostrychophila. The results indicated that the essential oil of A. maximum fruits had the potential to be developed as a natural insecticide and repellent for control of T. castaneum and L. bostrychophila.
- Published
- 2015
31. Bioactivity of Essential Oil from Artemisia stolonifera (Maxim.) Komar. and Its Main Compounds against Two Stored-Product Insects
- Author
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Cheng-Fang Wang, Wen-Juan Zhang, Zhu-Feng Geng, Shu-Shan Du, Wu Yan, Chun-Xue You, Kai Yang, Zhi-Wei Deng, Ying Wang, and Yang Su
- Subjects
General Chemical Engineering ,fungi ,Food storage ,General Medicine ,General Chemistry ,Biology ,biology.organism_classification ,law.invention ,Toxicology ,chemistry.chemical_compound ,Camphor ,Eucalyptol ,chemistry ,law ,Toxicity ,Lasioderma serricorne ,Flame ionization detector ,Food science ,Gas chromatography–mass spectrometry ,Essential oil - Abstract
Artemisia stolonifera, a perennial herb, is widely distrbuted in China. The aim of this study was to analyze the essential oil from the aerial parts of Artemisia stolonifera, as well as to evaluate the bioactivity of the oil and its main constituents. The essential oil was analyzed by gas chromatography-flame ionization detector and gas chromatography-mass spectrometry that allowed characterizing 22 compounds. The main components were eucalyptol (32.93%), β-pinene (8.18%), camphor (6.12%) and terpinen-4-ol (6.11%), and obtained from the essential oil after a further isolation. During the contact toxicity tests, the essential oil (LD50 = 8.60 μg/adult) exhibited stronger toxicity against Tribolium castaneum adults than those isolated constituents, however, camphor and terpinen-4-ol showed 1 and 2 times toxicity against Lasioderma serricorne adults than the essential oil (LD50 = 12.68 μg/adult) with LD50 values of 11.30 and 5.42 μg/adult, respectively. In the fumigant toxicity tests, especially on Tribolium castaneum, the essential oil (LC50 = 1.86 mg/L air) showed almost the same level toxicity as positive control, methyl bromide (LC50 = 1.75 mg/L air). Moreover, the essential oil and its four isolated constituents also exhibited strong repellency against two stored-product insects.
- Published
- 2015
32. Contact and Repellant Activities of Zerumbone and Its Analogues from the Essential Oil of Zingiber zerumbet (L.) Smith against Lasioderma serricorne
- Author
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Yan, Wu, Shan-Shan, Guo, Dong-Ye, Huang, Cheng-Fang, Wang, Jian-Yu, Wei, Zhi-Hua, Li, Jian-Sheng, Sun, Jia-Feng, Bai, Zhao-Fu, Tian, Ping-Juan, Wang, and Shu-Shan, Du
- Subjects
Coleoptera ,Insecticides ,Zingiberaceae ,Insect Repellents ,Monoterpenes ,Animals ,Plant Oils ,Sesquiterpenes - Abstract
The contact toxicity and repellent activities of the essential oil extracted from the rhizomes of Zingiber zerumbet (L.) Smith (Zingiberaceae) was evaluated against cigarette beetles (Lasioderma serricorne). The essential oil obtained by hydrodistillation was investigated by GC-FID and GC-MS. The main constituents of the essential oil were zerumbone (40.2%), α-caryophyllene (8.6%), humulene epoxide II (7.3%), camphene (5.9%) and fenchene (4.7%). Zerumbone and its analogues totally are accounting for 60.3% of the essential oil. It was found that the essential oil possessed contact toxicity against L. serricorne adults with a LD
- Published
- 2017
33. Bioactivity of essential oil of Litsea cubeba from China and its main compounds against two stored product insects
- Author
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Shan Shan Guo, Zhi Long Liu, Chun Xue You, Zhi-Wei Deng, Shu-Shan Du, Rui Qi Sun, Zhu Feng Geng, Kai Yang, and Cheng Fang Wang
- Subjects
Limonene ,biology ,Liposcelis bostrychophila ,Litsea cubeba ,biology.organism_classification ,Citral ,Insecticidal activity ,Essential oil ,law.invention ,DEET ,chemistry.chemical_compound ,Linalool ,chemistry ,law ,Insect Science ,Botany ,Lasioderma serricorne ,Repellency ,Food science - Abstract
During our screening program for agrochemicals from Chinese medicinal herbs and wild plants, the essential oil of Litsea cubeba fruits was found to possess strong contact toxicity against the cigarette beetle Lasioderma serricorne adults and the booklouse Liposcelis bostrychophila , with LD 50 values of 27.33 μg/adult and 71.56 μg/cm 2 , respectively, and also showed strong fumigant toxicity against the two stored product insects with LC 50 values of 22.97 and 0.73 mg/L, respectively. The essential oil obtained by hydrodistillation was investigated by GC MS. The main components of the essential oil were identified to be E-citral (geranial) (27.49%), Z-citral (neral) (23.57%) and d -limonene (18.82%) followed by β-thujene (3.34%), β-pinene (2.85%), α-pinene (2.57%), 6-methyl-5-hepten-2-one (2.40%) and linalool (2.36%). Citral (Z/E-citral), d -limonene, β-pinene, α-pinene and linalool were separated and purified by silica gel column chromatography and preparative thin layer chromatography, and further identified by means of physicochemical and spectrometric analysis. Citral and linalool showed strong contact toxicity against L. serricorne and L. bostrychophila (LD 50 = 11.76, 12.74 μg/adult and 20.15, 99.97 μg/cm 2 , respectively) and fumigant toxicity against L. serricorne and L. bostrychophila (16.54, 18.04 mg/L air and 0.14, 0.71 mg/L air, respectively). Otherwise, citral, d -limonene and linalool were strongly repellent against the cigarette beetle L. serricorne as the essential oil whereas β-pinene and α-pinene exhibited weaker repellency against the cigarette beetle compared with the positive control, DEET. Moreover, except α-pinene and linalool, the other three compounds as well as the essential oil exhibited comparable repellency against the booklouse relative to DEET.
- Published
- 2014
- Full Text
- View/download PDF
34. Study on Prediction Model of Springback Based on Support Vector Machine
- Author
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Shao Juan Su, Cheng Fang Wang, and Yong Hu
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Engineering ,Artificial neural network ,Bending (metalworking) ,business.industry ,Process (computing) ,General Medicine ,computer.software_genre ,Support vector machine ,Set (abstract data type) ,Effective method ,Data mining ,MATLAB ,business ,computer ,Predictive modelling ,computer.programming_language - Abstract
Accurate prediction and effective control springback has a great significance to the plate cold bending forming. Based on the analysis of the support vector machine method principle and structure, Study on support vector machine prediction process based on MATLAB and prediction models about radius and resilience is set up. And the results predicted were compared with BP neural network prediction results to verify the accuracy.To provide an effective method for the accurate prediction springback.
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- 2014
35. Two new cytotoxic constituents from the Clausena lansium (Lour.) Skeels
- Author
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Hai-Bo Yin, Shu-Shan Du, Li Fan, Cheng-Fang Wang, Chun-Xue You, Wen-Juan Zhang, Jiang-Bin Feng, Jing Chen, Ying-Jie Yang, Zhi-Wei Deng, Kai Yang, and Hai-Yan Jiang
- Subjects
biology ,Chemistry ,Stereochemistry ,Cancer ,Tumor cells ,Plant Science ,medicine.disease ,biology.organism_classification ,Biochemistry ,Clausine D ,medicine ,Cytotoxic T cell ,Clausena lansium ,Cytotoxicity ,Agronomy and Crop Science ,Human breast ,Two-dimensional nuclear magnetic resonance spectroscopy ,Biotechnology - Abstract
Two new compounds 6-methoxy-9H-carbazole-3-carboxylic acid ( 1 ) and 9-[3-methyl-4-(4-methyl-5-oxo-tetrapydro-furan-2-yl)-but-2-enyloxy]-furo[3,2-g]chromen-7-one ( 5 ) along with four known compounds clausine D ( 2 ), claulansines J ( 3 ), O-demethylmurrayanine ( 4 ) and pabularinone ( 6 ) were isolated from the Clausena lansium . The chemical structures of the new compound were elucidated by 1D and 2D NMR as well as HR-ESIMS spectral analysis. All the compounds isolated were evaluated for cytotoxic effects against human breast cancer (MCF-7), non-small lung carcinoma (H1299) and liver cancer (SMMC-7721). Compounds 2 and 4 exhibited strong cytotoxicity against MCF-7 and SMMC-7721 with IC 50 values in the range 2.63–7.59 μg/ml, and Compounds 1 and 5 showed moderate cytotoxicity against MCF-7, H1299 and SMMC-7721 tumor cell lines.
- Published
- 2014
36. Hull Plate Bending Springback Prediction Based on Artificial Neural Network
- Author
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Yong Hu, Bin Liu, Cheng Fang Wang, and Shao Juan Su
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Engineering ,Artificial neural network ,Bending (metalworking) ,business.industry ,Hull ,General Engineering ,Process (computing) ,Forming processes ,Automatic processing ,Structural engineering ,Bending of plates ,business ,Surface plate - Abstract
In the process of three-dimensional curved hull plate forming, springback caused serious influence on the forming accuracy, in order to ensure the forming quality of the asymmetric multiple pressure heads CNC bending machine of ship hull 3D surface plate, to achieve the automatic processing, it is necessary to solve the problem of springback in the hull plate forming process. It is rarely to see the research on the cold bending springback problem of middle-thickness hull plate now. To established nonlinear model of plate parameters and springback amount based on BP neural network, accurately analyzing the prediction of springback, and getting the sptringback prediction model based on the BP neural network in the Matlab programming.
- Published
- 2014
37. Chemical Constituents and Insecticidal Activities of the Essential Oil from Amomum tsaoko against Two Stored-Product Insects
- Author
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Zhi-Wei Deng, Ying Wang, Kai Yang, Chun-Xue You, Shu-Shan Du, Wen-Juan Zhang, Ran Chen, Zhu-Feng Geng, and Cheng-Fang Wang
- Subjects
Insecticides ,General Chemical Engineering ,Food storage ,Gas Chromatography-Mass Spectrometry ,Amomum tsaoko ,law.invention ,Lethal Dose 50 ,chemistry.chemical_compound ,Food Parasitology ,law ,Cyclohexenes ,Oils, Volatile ,Animals ,Food science ,Essential oil ,Tribolium ,Limonene ,Eucalyptol ,biology ,Terpenes ,Chemistry ,General Medicine ,General Chemistry ,Cyclohexanols ,biology.organism_classification ,Coleoptera ,Food Storage ,Monoterpenes ,Lasioderma serricorne ,Gas chromatography–mass spectrometry ,Edible Grain ,Undecane ,Amomum - Abstract
The aim of this research was to determine the chemical constituents and toxicities of the essential oil derived from Amomum tsaoko Crevost et Lemarie fruits against Tribolium castaneum (Herbst) and Lasioderma serricorne (Fabricius). Essential oil of A. tsaoko was obtained from hydrodistillation and was investigated by gas chromatography-mass spectrometry (GC-MS). GC-MS analysis of the essential oil resulted in the identification of 43 components, of which eucalyptol (23.87%), limonene (22.77%), 2-isopropyltoluene (6.66%) and undecane (5.74%) were the major components. With a further isolation, two active constituents were obtained from the essential oil and identified as eucalyptol and limonene. The essential oil and the two isolated compounds exhibited potential insecticidal activities against two storedproduct insects. Limonene showed pronounced contact toxicity against both insect species (LD50 = 14.97 μg/adult for T. castaneum; 13.66 μg/adult for L. serricorne) and was more toxic than eucalyptol (LD50 = 18.83 μg/adult for T. castaneum; 15.58 μg/adult for L. serricorne). The essential oil acting against the two species of insects showed LD50 values of 16.52 and 6.14 μg/adult, respectively. Eucalyptol also possessed strong fumigant toxicity against both insect species (LC50 = 5.47 mg/L air for T. castaneum; 5.18 mg/L air for L. serricorne) and was more toxic than limonene (LC50 = 6.21 mg/L air for T. castaneum; 14.07 mg/L air for L. serricorne), while the crude essential oil acting against the two species of insects showed LC50 values of 5.85 and 8.70 mg/L air, respectively. These results suggested that the essential oil of A. tsaoko and the two compounds may be used in grain storage to combat insect pests.
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- 2014
38. Square Head Adjustable Die Device Research on Ship 3D CNC Forming Machine
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Bo Liu, Shao Juan Su, Cheng Fang Wang, and Yong Hu
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Engineering ,business.product_category ,Bending (metalworking) ,business.industry ,Process (computing) ,Forming processes ,General Medicine ,Structural engineering ,Hull ,Indentation ,Numerical control ,Die (manufacturing) ,Head (vessel) ,business - Abstract
Research device structure upon suppressing impress and wrinkling about three-dimensional numerical control bending machine.3D ship curved-surface outside plate automatic forming process is always the difficult part in the field of domestic and international shipbuilding. Analyzing the problems in existing processing methods ,for example, line heating technology has the defects of poor production conditions, large labor intensity, not environmental friendly and cold bending forming method may result in indentation, wrinkling or rebound phenomenon.Presents the forming process of a ship hull plate based on square head adjustable die curved surface forming device. By setting the non pressure and a plurality of planar head to replace the traditional integral die method, the paper will illustrate the composition and working principles of the process at the same time try to implement effective control of indentation and wrinkle phenomenon followed by verification through experiments.
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- 2013
39. Research on the Detection Unit and Control System of CNC Cool Frame Bender
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Cheng Fang Wang and Yong Qing Zhou
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Engineering ,business.industry ,Control system ,Frame (networking) ,General Engineering ,Process (computing) ,business ,Unit (ring theory) ,Computer hardware ,Control methods - Abstract
Based on chord length measurement control method, the research on the key technology for the CNC cool Frame bender has been carried out. Detecting unit has been designed, and analyzing of the detecting objects, its range, parameters converting method are also described. Based on which the NC control system is developed, and also the method for the calculation control parameter and process of frame are described.
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- 2013
40. Antioxidant Phenolic Compounds from Pu-erh Tea
- Author
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Cheng Fang Wang, Sheng Min Shen, Zi Mu Liu, Gang Li Wang, Zhi-Wei Deng, Hai Ming Zhang, Shu-Shan Du, Zhi Long Liu, Peng Liu, and Yong Yan Wang
- Subjects
Pu-erh tea ,DPPH˙ ,ABTS˙+ ,chemical constituent ,antioxidant activity ,Antioxidant ,DPPH ,medicine.medical_treatment ,Pharmaceutical Science ,DPPH˙ ,+ ,Antioxidants ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Phenols ,Picrates ,Drug Discovery ,medicine ,+ ,ABTS%26#729%22"> ABTS˙ Benzothiazoles ,Gallic acid ,Physical and Theoretical Chemistry ,Scavenging ,Flavonoids ,ABTS ,Chromatography ,Tea ,Biphenyl Compounds ,Organic Chemistry ,Free Radical Scavengers ,Dpph scavenging ,chemistry ,Chemistry (miscellaneous) ,Molecular Medicine ,Sulfonic Acids ,Quercetin ,Kaempferol ,Oxidation-Reduction ,Drugs, Chinese Herbal ,Nuclear chemistry - Abstract
Eight compounds were isolated from the water extract of Pu-erh tea and their structures were elucidated by NMR and MS as gallic acid (1), (+)-catechin (2), (−)-epicatechin (3), (−)-epicatechin-3-O-gallate (4), (−)-epigallocatechin-3-O-gallate (5), (−)-epiafzelechin- 3-O-gallate (6), kaempferol (7), and quercetin (8). Their in vitro antioxidant activities were assessed by the DPPH and ABTS scavenging methods with microplate assays. The relative order of DPPH scavenging capacity for these compounds was compound 8 > compound 7 > compound 1 > compound 6 > compound 4 ≈ compound 5 > compound 2 > VC (reference) > compound 3, and that of ABTS scavenging capacity was compound 1 > compound 2 > compound 7 ≈ compound 8 > compound 6 > compound 5 > compound 4 > VC (reference) > compound 3. The results showed that these phenolic compounds contributed to the antioxidant activity of Pu-erh tea.
- Published
- 2012
41. Chemical Composition of Essential Oils from Zanthoxylum bungeanum Maxim. and Their Bioactivities against Lasioderma serricorne
- Author
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Wen-Juan, Zhang, Shan-Shan, Guo, Chun-Xue, You, Zhu-Feng, Geng, Jun-Yu, Liang, Zhi-Wei, Deng, Cheng-Fang, Wang, Shu-Shan, Du, and Yong-Yan, Wang
- Subjects
Coleoptera ,Zanthoxylum ,Insecticides ,Insect Repellents ,Fatty Acids ,Monoterpenes ,Animals ,Plant Oils ,Chromatography, Supercritical Fluid ,Sesquiterpenes ,Gas Chromatography-Mass Spectrometry ,Distillation - Abstract
Two essential oil samples were obtained from the pericarp of Zanthoxylum bungeanum with the methods of hydrodistillation (HD) and supercritical fluid CO
- Published
- 2016
42. Contact and Repellent Activities of the Essential Oil from Juniperus formosana against Two Stored Product Insects
- Author
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Chun-Xue You, Cheng-Fang Wang, Junyu Liang, Wen-Juan Zhang, Shu-Shan Du, Zhu-Feng Geng, and Shan-Shan Guo
- Subjects
0106 biological sciences ,Insecticides ,Pharmaceutical Science ,01 natural sciences ,Analytical Chemistry ,law.invention ,Terpene ,DEET ,chemistry.chemical_compound ,law ,Drug Discovery ,Food science ,contact toxicity ,Bicyclic Monoterpenes ,Tribolium ,biology ,Liposcelis bostrychophila ,Chemistry (miscellaneous) ,Molecular Medicine ,Food storage ,Cyclohexane Monoterpenes ,Article ,lcsh:QD241-441 ,Tribolium castaneum ,lcsh:Organic chemistry ,Botany ,Cyclohexenes ,Oils, Volatile ,Animals ,Humans ,Physical and Theoretical Chemistry ,Essential oil ,Limonene ,Juniperus formosana ,repellency ,Terpenes ,Organic Chemistry ,biology.organism_classification ,Plant Leaves ,010602 entomology ,chemistry ,Food Storage ,Insect Repellents ,Juniperus ,Monoterpenes ,010606 plant biology & botany - Abstract
The chemical composition of the essential oil from Juniperus formosana leaves and its contact and repellent activities against Tribolium castaneum and Liposcelis bostrychophila adults were investigated. The essential oil of J. formosana leaves was obtained by hydrodistillation and analyzed by GC-MS. A total of 28 components were identified and the main compounds in the essential oil were α-pinene (21.66%), 4-terpineol (11.25%), limonene (11.00%) and β-phellandrene (6.63%). The constituents α-pinene, 4-terpineol and d-limonene were isolated from the essential oil. It was found that the essential oil exhibited contact activity against T. castaneum and L. bostrychophila adults (LD50 = 29.14 μg/adult and 81.50 µg/cm2, respectively). The compound 4-terpineol exhibited the strongest contact activity (LD50 = 7.65 μg/adult). In addition, data showed that at 78.63 nL/cm2, the essential oil and the three isolated compounds strongly repelled T. castaneum adults. The compounds α-pinene and d-limonene reached the same level (Class V) of repellency as DEET (p = 0.396 and 0.664) against L. bostrychophila at 63.17 nL/cm2 after 2 h treatment. The results indicate that the essential oil and the isolated compounds have potential to be developed into natural insecticides and repellents to control insects in stored products.
- Published
- 2016
43. Study on the Numerical Control System for Frame Cold-Bending Machine
- Author
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Yong Hu, Pei Yong Lee, Cheng Fang Wang, Yun Sheng Mao, and Yong Qing Zhou
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Engineering ,Software ,Shipbuilding ,Bending (metalworking) ,business.industry ,Control system ,Frame (networking) ,General Engineering ,Mechanical engineering ,Shipyard ,business ,Numerical control system - Abstract
Shape processing of frame is one of the important and trouble working procedures for shipyards, and its robotized research has been carried on until it is gradually put into use in recent years. This paper introduces a CNC frame cold-bending machine, including the components of its machine, the principle of its NC control, the hardware and software of its NC control system.
- Published
- 2012
44. Repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of Acorus calamus rhizomes
- Author
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Qian Cai, Li-Shi Zheng, Cheng-Fang Wang, Kai Yang, Chun-Xue You, and Hai-Ping Chen
- Subjects
biology ,Traditional medicine ,Chemistry ,repellency ,Pharmaceutical Science ,Context (language use) ,Acorus calamus ,biology.organism_classification ,Median lethal dose ,law.invention ,Rhizome ,Toxicology ,Tribolium castaneum ,law ,Calamus ,Drug Discovery ,Lasioderma serricorne ,Original Article ,Gas chromatography ,contact toxicity ,Essential oil - Abstract
Context: It was found that the essential oil of Acorus calamus rhizomes showed insecticidal activity. Aim: The aim of this study was to determine the chemical composition of the essential oil from A. calamus rhizomes, evaluate insecticidal and repellant activity against Lasioderma serricorne (LS) and Tribolium castaneum (TC), and to isolate any insecticidal constituents from the essential oil. Materials and Methods: Essential oil from A. calamus was obtained by hydrodistillation and analyzed by gas chromatography (GC) flame ionization detector and GC-mass spectrometry. The insecticidal and repellant activity of the essential oil and isolated compounds was tested using a variety of methods. Results: The main components of the essential oil were identified to be isoshyobunone (15.56%), β-asarone (10.03%), bicyclo[6.1.0]non-1-ene (9.67%), shyobunone (9.60%) and methylisoeugenol (6.69%). Among them, the two active constituents were isolated and identified as shyobunone and isoshyobunone. The essential oil showed contact toxicity against LS and TC with LD 50 values of 14.40 and 32.55 μg/adult, respectively. The isolated compounds, shyobunone and isoshyobunone also exhibited strong contact toxicity against LS adults with LD 50 values of 20.24 and 24.19 μg/adult, respectively, while the LD 50 value of isoshyobunone was 61.90 μg/adult for TC adults. The essential oil, shyobunone and isoshyobunone were strongly repellent (98%, 90% and 94%, respectively, at 78.63 nL/cm 2 , after 2 h treatment) against TC. Conclusion: The essential oil, shyobunone and isoshyobunone possessed insecticidal and repellant activity against LS and TC.
- Published
- 2015
45. Chemical Constituents and Activity of Murraya microphylla Essential Oil against Lasioderma serricorne
- Author
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Chun-Xue, You, Shan-Shan, Guo, Wen-Juan, Zhang, Kai, Yang, Cheng-Fang, Wang, Zhu-Feng, Geng, Shu-Shan, Du, Zhi-Wei, Deng, and Yong-Yan, Wang
- Subjects
Coleoptera ,Insecticides ,Insect Repellents ,Oils, Volatile ,Animals ,Plant Oils ,Murraya - Abstract
The chemical composition, contact and repellent activities of the essential oil from Murraya microphylla branches and leaves against Lasioderma serricorne adults were determined and six compounds from the essential oil were isolated as well. The essential oil of M microphylla obtained by hydrodistillation was analyzed by gas chromatography-mass spectrometric (GC-MS) analysis; 22 compounds were identified. The main constituents of the essential oil included β-caryophyllene (18.0%), α-pinene (13.8%), spathulenol (9.5%), α-humulene (6.0%), γ-elemene (5.1%) and zingiberene (4.6%), followed by α-cadinol (3.9%) and caryophyllene oxide (3.8%). Six of these compounds were isolated and fully identified as α-pinene, β-caryophyllene, α-humulene, caryophyllene oxide, spathulenol and α-cadinol. L. serricorne adults had different sensitivities to the crude essential oil and isolated compounds. α-Humulene exhibited the strongest contact activity against L. serricorne, showing an LD50 value of 13.1 µg adult(-1). However, spathulenol, the crude essential oil and α-cadinol showed stronger contact activity against L. serricorne than caryophyllene oxide and β-caryophyllene. The essential oil, α-humulene and spathulenol showed comparable repellency against L. serricorne adults at 2 h after exposure, relative to the positive control, DEET. The results demonstrate that the essential oil and isolated compounds exhibited important contact and repellent activities against L. serricorne. Thus, they could become potential natural insecticides or repellents for control of insects in stored products.
- Published
- 2015
46. Essential oil composition and larvicidal activity of Saussurea lappa roots against the mosquito Aedes albopictus (Diptera: Culicidae)
- Author
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Shu-Shan Du, Sha Sha Chu, Qing He, Zhi Long Liu, Cheng Fang Wang, and Zhi-Wei Deng
- Subjects
Saussurea ,Insecticides ,Aedes albopictus ,Fractionation ,Sesquiterpene lactone ,Plant Roots ,Gas Chromatography-Mass Spectrometry ,law.invention ,chemistry.chemical_compound ,Aedes ,law ,Botany ,Oils, Volatile ,Animals ,Essential oil ,Saussurea lappa ,chemistry.chemical_classification ,Costunolide ,General Veterinary ,biology ,Traditional medicine ,Plant Extracts ,General Medicine ,biology.organism_classification ,Survival Analysis ,Infectious Diseases ,chemistry ,Larva ,Insect Science ,Biological Assay ,Parasitology - Abstract
In recent years, uses of environment friendly and biodegradable natural insecticides of plant origin have received renewed attention as agents for vector control. The aim of this research was to determine larvicidal activity of the essential oil derived from roots of Saussurea lappa (Compositae) and the isolated constituents against the larvae of the Culicidae mosquito Aedes albopictus. Essential oil of S. lappa roots was obtained by hydrodistillation and analyzed by gas chromatography (GC) and GC-mass spectrometry (MS). A total of 39 components of the essential oil of S. lappa roots were identified. The essential oil has higher content of (79.80%) of sesquiterpenoids than monoterpenoids (13.25%). The principal compounds in S. lappa essential oil were dehydrocostus lactone (46.75%), costunolide (9.26%), 8-cedren-13-ol (5.06%), and α-curcumene (4.33%). Based on bioactivity-directed fractionation, dehydrocostus lactone and costunolide were isolated from S. lappa essential oil. Dehydrocostus lactone and costunolide exhibited strong larvicidal activity against A. albopictus with LC(50) values of 2.34 and 3.26 μg/ml, respectively, while the essential oil had an LC(50) value of 12.41 μg/ml. The result indicated that the essential oil of S. lappa and the two isolated constituents have potential for use in control of A. albopictus larvae and could be useful in search of newer, safer and more effective natural compounds as larvicides.
- Published
- 2011
47. Toxicity of the Essential Oil ofIllicium difengpiStem Bark and Its Constituent Compounds Towards Two Grain Storage Insects
- Author
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Cheng Fang Wang, Shu-Shan Du, Sha Sha Chu, Shao Liang Liu, and Zhi Long Liu
- Subjects
Insecticides ,Article ,fumigant ,Illicium ,law.invention ,Tribolium castaneum ,chemistry.chemical_compound ,Food Parasitology ,Linalool ,law ,Botany ,Oils, Volatile ,Plant Bark ,Animals ,Red flour beetle ,contact toxicity ,Essential oil ,Tribolium ,biology ,Sitophilus ,General Medicine ,biology.organism_classification ,Horticulture ,chemistry ,Safrole ,Maize weevil ,Insect Science ,Weevils ,Edible Grain ,Sitophilus zeamais - Abstract
During our screening program for new agrochemicals from Chinese medicinal herbs, the essential oil of Illicium difengpi stem bark was found to possess strong insecticidal activities against the maize weevil, Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) and red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). A total of 37 components of the essential oil of I. difengpi were identified. The main components of the essential oil were safrole (23.61%), linalool (12.93%), and germacrene D (5.35%). Bioactivities-directed chromatographic separation on repeated silica gel columns led to the isolation of two compounds: safrole and linalool. Safrole showed pronounced contact toxicity against both insect species and (LD₅₀ = 8.54 for S. zeamais; 4.67 µg/adult for T. castaneum) and was more toxic than linalool (LD₅₀ = 24.88 for S. zeamais; 8.12 µg/adult for T. castaneum). The essential oil acting against the two species of insects showed LD₅₀ values of 13.83 and 6.33 µg/adult, respectively. Linalool also possessed strong fumigant toxicity against both insect species (LC₅₀ = 10.02 for S. zeamais; 9.34 mg/L for T. castaneum) and was more toxic than safrole (LD₅₀ = 32.96 and 38.25 mg/L), while the crude essential oil acting against the two species of insects showed LC₅₀ values of 14.62 and 16.22 mg/L, respectively. These results suggest that the essential oil of I. difengpi stem bark and the two compounds may be used in grain storage to combat insect pests.
- Published
- 2011
48. Antifeedant Diterpenoids against Tribolium castaneum from the Stems and Twigs of Ceriops tagal (Rhizophoraceae)
- Author
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Zhi-Wei Deng, Hai Ming Zhang, Shu-Shan Du, Zhi Long Liu, Jing Li, Qi Zhi Liu, and Cheng Fang Wang
- Subjects
Insecticides ,animal structures ,Magnetic Resonance Spectroscopy ,media_common.quotation_subject ,Pharmaceutical Science ,Insect ,Ceriops tagal ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,Tribolium castaneum ,lcsh:Organic chemistry ,Drug Discovery ,Botany ,feeding deterrents ,tagalsin ,Animals ,Red flour beetle ,Physical and Theoretical Chemistry ,Spectral data ,media_common ,Mangrove plants ,Tribolium ,biology ,Molecular Structure ,Plant Stems ,Plant Extracts ,Organic Chemistry ,fungi ,food and beverages ,Rhizophoraceae ,Pesticide ,biology.organism_classification ,Coleoptera ,Phytochemical ,Chemistry (miscellaneous) ,Molecular Medicine ,Diterpenes - Abstract
The screening of several Chinese mangrove plants for insecticidal principles showed that ethanol extract of Ceriops tagal stems and twigs possessed significant feeding deterrent activity against the red flour beetle, Tribolium castaneum (Family: Rhizophoraceae). From the ethanol extract, three feeding deterrent diterpenoids were isolated by bioassay-guided fractionation. The compounds were identified as tagalsin A, B, and H on the basis of their phytochemical and spectral data. Tagalsin A, B, and H exhibited strong feeding deterrent activity against T. castaneum adults with EC 50 values of 375.3 ppm, 277.3 ppm, and 285.45 ppm, respectively. Keywords: feeding deterrents; Ceriops tagal ; Tribolium castaneum ; tagalsin 1. Introduction The red flour beetle [ Tribolium castaneum (Herbst)] is one of the most widespread and destructive primary insect pests of stored cereals [1]. Infestations not only cause significant losses due to the consumption of grains; they also result in elevated temperature and moisture conditions that lead to an accelerated growth of molds, including toxigenic species [2]. Botanical pesticides have the advantage of providing novel modes of action against insects that can reduce the risk of cross-resistance as well
- Published
- 2011
49. Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves
- Author
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Shu-Shan Du, Cheng Fang Wang, Rui Fang, Yong Yan Wang, Ligang Zhou, Zhi Long Liu, Zhi-Wei Deng, Hai Ming Zhang, Jie Cao, and Kai Yang
- Subjects
Zanthoxylum ,Insecticides ,Magnetic Resonance Spectroscopy ,linalool ,sabinene ,Pharmaceutical Science ,Sabinene ,Article ,Gas Chromatography-Mass Spectrometry ,fumigant ,essential oil ,Analytical Chemistry ,law.invention ,Lethal Dose 50 ,estragole ,lcsh:QD241-441 ,chemistry.chemical_compound ,Linalool ,lcsh:Organic chemistry ,law ,Zanthoxylum schinifolium ,Sitophilus zeamais ,contact toxicity ,Drug Discovery ,Botany ,Nerol ,Oils, Volatile ,Animals ,Food science ,Physical and Theoretical Chemistry ,Essential oil ,Limonene ,biology ,Sitophilus ,Organic Chemistry ,biology.organism_classification ,Plant Leaves ,chemistry ,Chemistry (miscellaneous) ,Chromatography, Gel ,Molecular Medicine ,Weevils ,Estragole - Abstract
In our screening program for new agrochemicals from Chinese medicinal herbs and wild plants, Zanthoxylum schinifolium essential oils were found to possess strong insecticidal activity against the maize weevil Sitophilus zeamais. The essential oils of Z. schinifolium fruits and leaves were extracted via hydrodistillation and investigated by GC and GC-MS. Estragole (69.52%) was the major compound of the essential oil of fresh fruits, followed by linalool (8.63%) and limonene (4.34%) and 94.33% of the total components were monoterpenoids. The main components of the essential oil of fresh leaves were linalool (12.94%), ar-tumerone (8.95%), limonene (6.45%) and elixene (5.43%) and only 50.62% were monoterpenoids. However, the essential oil from purchased fruits contained linalool (33.42%), limonene (13.66%) and sabinene (5.72%), followed by estragole (4.67%), nerol (4.56%) and 4-terpineol (4.27%). Estragole, linalool and sabinene were separated and purified by silica gel column chromatography and preparative thin layer chromatography, and further identified by means of physicochemical and spectrometric analysis. The essential oil from the fresh fruits (LD50 = 15.93 μg/adult) possessed two times more toxicity to the insects compared with that of fresh leaves (LD50 = 35.31 μg/adult). Estragole, linalool and sabinene exhibited contact activity against S. zeamais with LD50 values of 17.63, 13.90 and 23.98 μg/adult, respectively. The essential oils of Z . schinifolium possessed strong fumigant toxicity against S. zeamais adults with LC50 values of 13.19 mg/L (fresh fruits), 24.04 mg/L (fresh leaves) and 17.63 mg/L (purchased fruits). Estragole, linalool and sabinene also exhibited strong fumigant toxicity against the maize weevils with LC50 values of 14.10, 10.46 and 9.12 mg/L, respectively.
- Published
- 2011
50. Research of the Plastic Deformation Zone for Ship Frame Cold Forming
- Author
-
Yong-qing Zhou, Cheng-fang Wang, Pei-yong Li, Yun-sheng Mao, Jun-jie Song, and Zuquan Xiang
- Subjects
business.industry ,Frame (networking) ,Structural engineering ,business ,Cold forming ,Geology - Published
- 2015
Catalog
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