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In order to study the effect of Hericium erinaceus polysaccharide (HEP) on the immune and antioxidation functions of immunosuppressed mice. The control group received distilled water orally and the model and experimental groups I, II, and III received 0, 80, 160, and 320 mg/kg HEP respectively for a fortnight after re-molding with cyoclphosphnalide (CTX). Compared with the control group, the secretion of IL-2, IL-4, and IFN-γ, the activity or content of T-AOC, T-SOD, and GSH-PX, and the expression of PCNA mRNA in the thymus and spleen were reduced in immunosuppressed mice (P

This research is intended to study the effect of water flow on the release flux of DNAPLs, which have been deposited on the riverbed surface after sudden water pollution accidents. Those contaminants will slowly diffuse from the riverbed into the overlying water body through hydrodynamic action, causing ongoing and serious water pollution. By taking dichloromethane as a typical contaminant, the release form under different hydrodynamic conditions was observed through flume experiments, and the response relationship between the release flux and hydrodynamic factors was analyzed, with an emphasis on parameterizing the release rate. The results suggested that stronger water disturbance significantly enhanced the release of contaminants. And the relationship between the release flux and hydrodynamic factors generally followed an exponential distribution (R

Purpose: To describe the trajectories of health-risk behaviors (HRBs) among college students through four consecutive surveys and explore the relationship between chronotype, sleep duration and different trajectories of HRBs.Methods: We used a data sample of 1,042 college students from the College Student Behavior and Health Cohort Study. Students reported sleep parameters, including chronotype (Morning and Evening Questionnaire-5, MEQ-5) and sleep duration. The behavior scale was used to evaluate four HRBs (smoking, alcohol use, low physical activity, smartphone addiction). The latent class growth analysis (LCGA) was used to estimate the trajectory of self-reported HRBs. Multivariate logistic regression models were used to study whether sleep parameters (chronotype and sleep duration) correlated with HRBs' trajectories.Results: Four unique trajectories of behaviors were identified: unhealthy group (7.4%), increasing group (21.3%), decreasing group (10.3%) and healthy group (61.0%). Compared with the normal sleep, results from logistic regression analyses indicated that long sleep (>9 h) was associated with the decreasing group and the unhealthy group(pppConclusion: E-types and aberrant sleep duration (short and long sleep duration) were significantly associated with the trajectory of HRBs. Findings underscore the need for targeted screening and prevention of modifiable sleep behaviors with the aim of improving HRBs in college students.

We have explored the effect of boron in drinking water on the microstructure, mechanical barrier, immune barrier, antioxidant activity, and cell proliferation in rat jejunum. The treatment resulted in a significant increase in the jejunum villi height and ratio of villus height to crypt depth compared to the control group. The results showed that the jejunum villi height and ratio of villus height to crypt depth of the rats in experimental groups IV and V significantly increased compared with the control group (P < 0.05 or P < 0.01). Furthermore, boron treatment resulted in a significant increase in the number of rat jejunum goblet cells, intraepithelial lymphocyte and proliferating cell nuclear antigen positive cells as well as the concentration of secretory immunoglobulin A along with increased activity or content of total superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. However, there was a decrease in expression of these markers in rats receiving the highest dosage of boron (640 mg/L). The results show that supplementing drinking water with low doses of boron could improve the microstructure of rat jejunum, enhance mucosal immunity, mechanical barrier function, and antioxidant activity, and promote cell proliferation.

A new wheat–Leymus mollis (2n = 4x = 28, NsNsXmXm) disomic addition line M11003-3-1-12-10 (M10) was developed and selected from the BC1F5 progeny of a cross between wheat cv. 7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs). Cytological observations showed that M10 contained 44 chromosomes and formed 22 bivalents at meiotic metaphase I. The sequential fluorescence in situ hybridization–genomic in situ hybridization (FISH–GISH) analysis using the oligonucleotide Oligo-pSc119.2, Oligo-pTa535 and L. mollis genomic DNA as probes revealed that M10 carried a pair of L. mollis chromosomes and a whole set of 42 wheat chromosomes. Two EST–STS markers and six PLUG (PCR-based Landmark Unique Gene) markers confirmed that the added L. mollis chromosomes in M10 belonged to homoeologous group 7 and were designated as Lm#7Ns, so M10 was deduced to be a wheat–L. mollis Lm#7Ns disomic addition line. Field disease evaluation demonstrated that M10 showed a high level of resistance to stripe rust at the adult stage and the resistance could be ultimately traced to the L. mollis. Consequently, the introduced Lm#7Ns chromosomes were responsible for the stripe rust resistance. The novel disomic addition line M10 could be exploited as an important genetic resource for wheat resistance breeding.

As an emerging industry, laser processing technology has developed rapidly and has gradually become a key technology in transforming traditional manufacturing. It has been widely used in various fields such as industrial production, communication technology, information processing, health care, military, and scientific research. The application and development of laser processing technology is restricted by thermal damage and the processing residues existing in traditional laser processing. However, water laser compound machining can better solve the above-mentioned problems. In water laser compound machining , heat and byproducts can be absorbed and taken away by water to improve processing quality. This paper expounds and analyzes the principles and research of three popular water laser compound machining methods (water-guided laser processing, underwater laser processing and water-jet-assisted laser processing). Furthermore, this paper analyzes the technical difficulties in water laser compound machining and looks forward to the future development trends of this technology.

The effects of different olefin contents in fuel (nominally 8.2 and 17.2% in volume) on the performance and exhaust emissions of a modern gasoline direct injection engine at speeds of 1500, 3500, a...

A large eddy simulation of the turbulent syngas non-premixed jet flame of Sandia ETH/Zurich B is conducted using an in-house version of FireFOAM, a fire simulation solver within OpenFOAM. Combustio...

Mandatory continuing professional education is accepted across many professions as a re-credentialing mechanism to maintain professional competency. Self-directed learning is a widely recognized type of learning to meet mandatory continuing professional education requirements. The nature and characteristics of self-directed learning has been transformed with the growth in digital and mobile technologies, however there is minimal understanding of the role of these technologies in the self-directed learning habits of adult learners. This study sought to explore the perspectives of adult learners around the effect of digital and mobile technologies on continuing professional education activities. Semi-structured interviews were conducted with 55 adult learners from four professional groups (9 physicians; 20 nurses; 4 pharmacists; 22 social workers). Key thematic categories included perceptions of self-directed learning, self-directed learning resources, key triggers, and barriers to undertaking self-directed learning. Digital and mobile technologies emerged as important resources supporting the self-directed learning of health and human services professionals. Increasing usage and dependency on these technologies has important implications for organizational and workplace policies that can support effective self-directed learning processes in a digital age. A conceptual model is introduced to characterize the key factors defining the self-directed learning patterns and practices of adult learners in a digital age.

Flumes have been widely used in water conservancy science and environmental science research. It is of great significance to obtain the hydrodynamic characteristics and flow field uniformity in the flume. In this study, a new type of annular flume was taken as an example. The 3D flow field was simulated by using a commercial computational fluid dynamics (CFD) code, and was also measured by acoustic doppler velocimeter (ADV) to verify the simulation results. The average relative error range was between 8.37% and 9.95%, the simulated results basically reflected the actual situation of the flow field. On this basis, the structural characteristics of flow field were analyzed. A new calculation method of flow velocity uniformity was presented according to the flow characteristics of natural open channels. The velocity uniformity in the straight channel was calculated and analyzed based on this method, and the influence of speed on the velocity uniformity was further discussed. The length of uniform section was negatively correlated with the rotational speed (average velocity), which was between 39 cm and 101 cm in the straight, and the uniformity coefficient was less than 10%. Finally, the water flow characteristics in the straight channel without wheel were compared with the natural open channel flow. The longitudinal velocity was well fitted with the Prandtl logarithmic distribution formula (R2 &gt, 0.977), and the application feasibility of the flume was analyzed. This study can provide technical support for the development and application of annular flume.

The China VI natural gas engine is based on the theory of the air–fuel ratio method, leading to a high exhaust temperature, large heat load and difficult cooling. The paper carried out a thermal balance experiment of the China VI natural gas engine under different working conditions. A dual-loop organic Rankine cycle system is designed to reduce the difficulty of cooling system and improve the thermal efficiency. The high-temperature loop uses water as working fluid to recover the heat of engine exhaust gas and recirculation exhaust gas. The low-temperature loop recovers the heat from cooling water and waste heat of the high-temperature loop. The high-temperature and low-temperature loops are coupled through a shared heat exchanger. The results show that the China VI natural gas engine has a higher and more stable waste heat temperature than traditional engines and is more adaptable to waste heat recovery under variable operating conditions. For fixed evaporation temperature, with the increase of inlet temperature of the expander, the net output power of high-temperature loop increases first and then decreases, reaching the maximum value at about 800K. In the low-temperature loop working fluids R601a, R601b, R600, R600a and R12333zd, The maximum net output power of the waste heat recovery system based on the combination of water and R12333zd is approximately 75.4 kW under the rated condition, which is 22.24% of the rated power of the engine.

Supplementing different quantities of boron can significantly affect immune function in rat spleen, but the mechanism of action behind this effect remains unclear. Our purpose was to study the involvement of the estrogen membrane receptor GPR30 in the effect of boron on the proliferation, apoptosis, and immune function of rat spleen lymphocytes. Results showed that the addition of 0.4 mmol/L boron had a beneficial effect on the immune function and proliferation of spleen lymphocytes, but the addition of 40 mmol/L boron had opposite effect. After using G-15 to selectively inhibit GPR30, the proportions of CD4+ and CD8+ T cells, the content of IL-2 and IFN-γ, and the expression of PCNA protein were significantly decreased, while lymphocyte apoptosis rate increased significantly (p 0.05), while the addition of 40 mmol/L boron did not change the effects on lymphocyte subsets, proliferation and apoptosis. The results suggested that GPR30 mediates the effects of 0.4 mmol/L boron boron on the proliferation, apoptosis and immune function of spleen lymphocytes.

Boron is an essential trace element for animals. Appropriate boron supplementation can produce beneficial effects on the animal body, while a high dose of boron has adverse and even toxic effects. Our aim was to investigate the impact of different doses of boron on the microstructure of duodenum in rats, expression of secretory immunoglobulin A (SIgA) and tight junction protein, cell proliferation and apoptosis. Eighty newly weaned clean Sprague-Dawley (SD) rats were given distilled water supplemented with 0, 10, 20, 40, 80, 160, 320, and 640 mg/L of boron for 60 days. We found that supplementation of 40 and 80 mg/L boron could increase the height of duodenal villi and the crypt depth, the number of intraepithelial lymphocytes (IELs) and goblet cells, the expression of SIgA, Zonula occludens-1 (ZO-1) and occludin, and proliferating cell nuclear antigen (PCNA) in duodenum of rats; decrease expression of Caspase-3 mRNA and the number of Caspase-3-positive cells, but supplementation of 320 and 640 mg/L boron could have the opposite effect in these indexes. The results showed that supplemented with 40 and 80 mg/L of boron could improve the structure and function of duodenum, while supplemented with 320–640 mg/L had a significant inhibitory effect.

The aphid Sitobion avenae F. is one of the most harmful pests of wheat growth in the world. A primary field screening test was carried out to evaluate the S. avenae resistance of 527 wheat landraces from Shaanxi. The results indicated that 25 accessions (4.74%) were resistant to S. avenae in the three consecutive seasons, of which accession S849 was highly resistant, and seven accessions were moderately resistant. The majority of S. avenae resistant accessions come from Qinling Mountains. Then, the genetic variability of a set of 33 accessions (25 S. avenae resistant and 8 S. avenae susceptible) originating from Qinling Mountains have been assessed by 20 morphological traits and 99 simple sequence repeat markers (SSRs). Morphological traits and SSRs displayed a high level of genetic diversity within 33 accessions. The clustering of the accessions based on morphological traits and SSR markers showed significant discrepancy according to the geographical distribution, resistance to S. avenae and species of accessions. The highly and moderately resistant landrace accessions were collected from the middle and the east part of Qinling Mountains with similar morphology characters, for example slender leaves with wax, lower leaf area, and high ear density. These S. avenae resistant landraces can be used in wheat aphid resistance breeding as valuable resources.

The extension of the laminar smoke point based approach to turbulent combustion using the partially stirred reactor (PaSR) concept proposed by Chen et al. (2014) has been further improved to overcome the limitation in the formulations of Chen et al. (2014) which assumed infinitely fast soot oxidation chemistry and constant soot formation characteristic time. In the PaSR approach, each computational cell is split into two zones: the reacting zone and the non-reacting zone. Soot formation and oxidation are assumed to take place at finite rates in the reacting zone and computed from the corresponding laminar rates and the mass fractions for soot formation and oxidation, which are evaluated in each computational cell from the characteristic time scales for turbulent mixing, soot formation and oxidation. Since soot would be produced in not only the fine structures but also surrounding fluids in the Eddy-Dissipation-Concept (EDC) model, the average field parameters between the fine structure and surrounding fluid are employed instead of those Favre-averaged values in Chen et al.’s soot formation model. The newly extended model has been implemented in FireFOAM, a large eddy simulation (LES) based solver for fire simulation based on the open source CFD code OpenFOAM®. Numerical simulations of a 30 cm diameter heptane and toluene pool fires tested by Klassen and Gore (1992) were performed for validation. The predicted soot volume fraction and temperature have achieved improved agreement with the experimental measurements in comparison with that of Chen et al. (2014), demonstrating the potential of the improved PaSR-based soot model for fire applications.

In this study, a thermodynamic model of a hybrid system consisting of a proton-exchange membrane fuel cell (PEMFC) system and an organic Rankine cycle (ORC) system is established using Aspen Plus software. The optimal working fluid is selected by comparing the performance of pure working fluids and zeotropic mixtures of working fluids in an ORC system. The zeotropic working fluid consisting of R245fa/R123 with a mixing ratio of 0.6/0.4 shows the best performance. The effects of some key parameters, including the current density and operating temperature, on the performance of the PEMFC–ORC system with zeotropic mixing fluids are discussed. The results indicate that the power and efficiency of the PEMFC–ORC system with the zeotropic mixing fluid increase with increasing operating temperature. When the R245fa/R123 zeotropic working fluid with a mixing ratio of 0.6/0.4 is used, the net power and efficiency of the hybrid system reach optimum values under the studied boundaries, with improvements of 12.87% and 4.84%, respectively. The influence of the maximum allowable evaporation pressure and stack operating temperature on the selection of optimum working fluid for the hybrid system is discussed in addition.

The fungal plant pathogen, Fusarium graminearum, contains two genes, FgCPK1 and FgCPK2, encoding the catalytic subunits of cAMP-dependent protein kinase A. FgCPK1 and FgCPK2 are responsible for most of the PKA activities and have overlapping functions in various cellular processes in F. graminearum. The cpk1 cpk2 double mutant was significantly reduced in growth, rarely produced conidia, and was non-pathogenic. In this study, we found that the cpk1 cpk2 double mutant was unstable and produced fast-growing spontaneous sectors that were defective in plant infection. All spontaneous suppressor strains had mutations in FgSFL1, a transcription factor gene orthologous to SFL1 in yeast. Thirteen suppressor strains had non-sense mutations at Q501, three suppressor strains had frameshift mutations at W198, and five suppressor strains had mutations in the HSF binding domain of FgSfl1. Only one suppressor strain had both a non-synonymous mutation at H225 and a non-sense mutation at R490. We generated the SFL1 deletion mutant and found that it produced less than 2% of conidia than that of the wild-type strain PH-1. The sfl1 mutant was significantly reduced in the number of perithecia on carrot agar plates at 7 days post-fertilization (dpf). When incubated for more than 12 days, ascospore cirrhi were observed on the sfl1 mutant perithecia. The infection ability of the sfl1 deletion mutant was also obviously defective. Furthermore, we found that in addition to the S223 and S559 phosphorylation sites, FgSFL1 had another predicted phosphorylation site: T452. Interestingly, the S223 phosphorylation site was responsible for sexual reproduction, and the T452 phosphorylation site was responsible for growth and sexual reproduction. Only the S559 phosphorylation site was found to play an important role in conidiation, sexual reproduction, and infection. Overall, our results indicate that FgSFL1 and its conserved PKA phosphorylation sites are important for vegetative growth, conidiation, sexual reproduction, and pathogenesis in F. graminearum.

Methanol is widely used as a potential alternative source for diesel fuel and can reduce the CO, THC and smoke emissions of the CI engines. However, the lower cetane number and energy content of methanol hinder the high substitution of methanol over diesel fuel. Introducing nanoparticles as fuel-additive is an effective approach to cope with this issue. In the present work, the alumina, ceria and silica nanoparticles were separately mixed into methanol in mass proportions of 25, 50 and 100 ppm with the composite surfactant of sodium dodecyl benzene sulfonate and cetyl trimethyl ammonium bromide (1:1 mass fraction) to create the nanofluid. The nanofluid was then injected into the intake port to combust synergistically with the mineral diesel fuel injected by the other high-pressure injection system, generating the nanoparticle-assisted methanol-diesel fuel mode (NMF). The characteristics of combustion and pollutant emission of NMFs were investigated with 10%, 30% and 50% methanol substituted level (M10, M30 and M50, respectively), at 10–90% engine loads of a constant engine speed. The results showed that the substitution of methanol led to an extension in the ignition delay but a shortening of combustion duration than diesel fuel. The additional introduction of alumina and ceria nanoparticles in each dosage, and silica nanoparticles in 100 ppm dosage, into M10 slightly shortened the ignition delay at 10% load conditions. When dosed into M50 fuel, all the three nanoparticles in various dosages led to shortened ignition delay at 10–50% engine loads. Over the test conditions, the addition of nanoparticles all caused a distinct increase in the peak in-cylinder pressure for the methanol-diesel dual-fuel mode. However, the addition of nanoparticles exerted marginal influences on further reduction of CO, HC and smoke emissions, and even further elevated the NOx emissions for MDF mode by up to 40%. Moreover, the deterioration degrees of NOx emissions for alumina and ceria nanoparticle addition were higher than the silica nanoparticles, and elevated towards the higher nanoparticle dosage and lower engine load.

Fusarium graminearum is a causal agent of wheat scab disease and a producer of deoxynivalenol (DON) mycotoxins. Treatment with exogenous cyclic adenosine monophosphate (cAMP) increases its DON production. In this study, to better understand the role of the cAMP-protein kinase A (PKA) pathway in F. graminearum, we functionally characterized the PKR gene encoding the regulatory subunit of PKA. Mutants deleted of PKR were viable, but showed severe defects in growth, conidiation and plant infection. The pkr mutant produced compact colonies with shorter aerial hyphae with an increased number of nuclei in hyphal compartments. Mutant conidia were morphologically abnormal and appeared to undergo rapid autophagy-related cell death. The pkr mutant showed blocked perithecium development, but increased DON production. It had a disease index of less than unity and failed to spread to neighbouring spikelets. The mutant was unstable and spontaneous suppressors with a faster growth rate were often produced on older cultures. A total of 67 suppressor strains that grew faster than the original mutant were isolated. Three showed a similar growth rate and colony morphology to the wild-type, but were still defective in conidiation. Sequencing analysis with 18 candidate PKA-related genes in three representative suppressor strains identified mutations only in the CPK1 catalytic subunit gene. Further characterization showed that 10 of the other 64 suppressor strains also had mutations in CPK1. Overall, these results showed that PKR is important for the regulation of hyphal growth, reproduction, pathogenesis and DON production, and mutations in CPK1 are partially suppressive to the deletion of PKR in F. graminearum.

Fusarium graminearum is a causal agent of wheat scab and a producer of the trichothecene mycotoxin deoxynivalenol (DON). The expression of trichothecene biosynthesis (TRI) genes and DON production are mainly regulated by the cyclic adenosine monophosphate-protein kinase A (cAMP-PKA) pathway and two pathway-specific transcription factors (TRI6 and TRI10). Interestingly, deletion mutants of TRI6 show reduced expression of several components of cAMP signalling, including the FgCAP1 adenylate-binding protein gene that has not been functionally characterized in F. graminearum. In this study, we show that FgCap1 interacts with Fac1 adenylate cyclase and that deletion of FgCAP1 reduces the intracellular cAMP level and PKA activity. The Fgcap1 deletion mutant is defective in vegetative growth, conidiogenesis and plant infection. It also shows significantly reduced DON production and TRI gene expression, which can be suppressed by exogenous cAMP, indicating a PKA-dependent regulation of DON biosynthesis by FgCap1. The wild-type, but not tri6 mutant, shows increased levels of intracellular cAMP and FgCAP1 expression under DON-producing conditions. Furthermore, the promoter of FgCAP1 contains one putative Tri6-binding site that is important for its function during DON biosynthesis, but is dispensable for hyphal growth, conidiogenesis and pathogenesis. In addition, FgCap1 shows an actin-like localization to the cortical patches at the apical region of hyphal tips. Phosphorylation of FgCap1 at S353 was identified by phosphoproteomics analysis. The S353A mutation in FgCAP1 has no effect on its functions during vegetative growth, conidiation and DON production. However, expression of the FgCAP1S353A allele fails to complement the defects of the Fgcap1 mutant in plant infection, indicating the importance of the phosphorylation of FgCap1 at S353 during pathogenesis. Taken together, our results suggest that FgCAP1 is involved in the regulation of DON production via cAMP signalling and subjected to a feedback regulation by TRI6, but the phosphorylation of FgCap1 at S353 is probably unrelated to the cAMP-PKA pathway because the S353A mutation only affects plant infection.

Introduction Health and human services professionals are increasingly using mobile devices to support clinical decision-making and evidence-based practice. However, research on self-directed learning in an era of growing digital technology utilization is underdeveloped. This study explored the adoption and use of mobile learning as a continuing professional development (CPD) activity. Methods A mixed-methods case study using semistructured interviews and a web-based questionnaire was conducted with health and human services professionals in Newfoundland and Labrador, Canada. Results Respondents reported using a smartphone (53.8%), tablets (50.4%), YouTube (43.0%), and mobile apps (35.8%) for CPD. The highest-rated benefits of mobile learning included improved access to information (M = 3.51); potential for enhanced knowledge acquisition (M = 3.45); staying up to date (M = 3.44); and verifying information (M = 3.40). The greatest barriers included cost of some apps and resources (M = 3.07); websites/programs not functional on mobile devices (M = 2.84); workplace barriers preventing access to digital resources (M = 2.82); and social media use linked to negative perceptions of professionalism (M = 2.65). Interview respondents described the flexibility and convenience of mobile learning, the level of autonomy it offered, and the advantages of learning on their own time. Technical issues, particularly for rural and remote practitioners, and digital professionalism also emerged as potential barriers. Discussion A systems model organizes the factors influencing the adoption and use of mobile devices and resources to support "just-in-time" learning. Addressing policies, practices, and regulations that enable or inhibit adoption of mobile learning for CPD may foster enhanced use to support better clinical decision-making, improved accuracy, and greater patient safety.

Ustilaginoidins are a kind of mycotoxins with 9,9′-linked bis-naphtho-γ-pyrones structures produced by the rice false smut pathogen Villosiclava virens. These metabolites displayed a wide range of bioactivities, such as teratogenic, cytotoxic, phytotoxic, and antibacterial activities. So far 26 ustilaginoidins have been isolated from V. virens, among which 18 compounds contained stereogenic center(s), however, most of them were unknown for the absolute configurations, except that of ustilaginoidin D. In this study, the absolute structures of these ustilaginoidins were constructed for the first time by analysis of the biosynthetic monomers obtained from a gene knockout mutant (ΔUV_2091) of V. virens. The gene UV_2091 was predicted to encode an enzyme that dimerized the monomeric naphtho-γ-pyrones in V. virens. Knockout of this gene led to the accumulation of three monomers, namely hemiustilaginoidin F (1), epihemiustilaginoidin D (2), and hemiustilaginoidin D (3), but the production of ustilaginoidins was completely blocked. The structures of the monomers were deduced by spectroscopic analysis, in combination with TDDFT ECD calculations for determining the absolute configurations. These compounds were tested for their phytotoxic, cytotoxic, antibacterial, and antifungal activities. Compounds 1 and 3 showed inhibition against the radicle and plumule elongation of rice and lettuce seeds at the tested concentrations. Compound 1 was active against the tested five human cancer cells, with half maximal inhibitory concentrations (IC50s) of 13.2~37.3 μM. Compounds 1~3 inhibited the growth of the tested pathogenic bacteria with minimum inhibitory concentrations of 8~32 µg/mL, while compound 3 exhibited antifungal activity against Magnaporthe oryzae (IC50, 5.21 µg/mL). A comparison of these data with those of the ustilaginoidins provided insights into the structure-bioactivity relationships.

A mathematical model for simulating hydrodynamics and pollutants migration in a tidal river network was constructed, which takes the temporal and spatial distribution of rainfall runoff and non-point pollutants into consideration. Under the design hydrologic conditions of a typical hydrological year, the daily concentration change process for the control section is obtained. Aiming at the uncertainty of hydrology and water quality parameters such as flow direction, flow rate and concentration change in tidal river network area, a statistical analysis method is used to obtain the maximum allowable concentration of pollutants in the control section under the condition of the water quality standard assurance rate of. Then, a formula for calculating the pollutions emission intensity of point and non-point sources is derived. The method was applied to the pollution source control in a typical region like Taihu in China.