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In this paper, we use the krill swarm optimization algorithm to optimize the optimization-seeking ability of the projection-seeking analysis model and construct the IKH-PP model. Evaluation indexes of ideological and political education strategies are built from seven dimensions, such as moral quality and political performance, and the index values are standardized using the extreme value normalization method. Create and enhance the projection indicator function, sort based on the projection value size, and determine the advantages and disadvantages of various samples. Taking psychology as the perspective, efforts were made to explore a collaborative educational path for psychological counseling and ideological education for medical students using the psychological perspective. The results showed that 50% of the 600 medical students at University Z chose “realization of ideal pursuit,” and they were generally more positive and progressive. However, only 48 of them expressed that they were very concerned about current affairs and that their political awareness still needed to be improved. Before and after the collaborative psychological counseling and political education interventions, there were statistically significant differences between Group I and Group II in the G3/F3 dimension (P=0.047, 0.008) and the G11/F11 dimension (P=0.022, 0.034), which were statistically significant or not. There were statistically significant differences in the overall access to Civics and all dimensions of Civics among medical students of different genders (P

In the context of the new era, reforming “curriculum and political thinking” in the medical specialty of colleges and universities is a key path to realizing the fundamental task of cultivating moral integrity. The study adopts a blended teaching mode, combines online and offline resources, and focuses on analyzing the learning behavior of medical students. The correlation between learning behavior and performance was analyzed by DH-Kmeans cluster analysis algorithm and Pearson’s correlation coefficient. The experimental data came from the online learning behaviors of students in the 2022-2023 academic year of T Medical College, and a total of 2426 data were collected. The results showed that learning behaviors were highly correlated with grades, especially the number of tasks completed, course video progress, and the number of chapter studies. In addition, the comparative analysis of teaching experiments showed that students in the experimental class were better than the control class regarding ideological values, learning attitudes and grades. The conclusion shows that the blended teaching mode effectively improves medical students’ learning effect and the quality of ideological education.

Aim: The main focus of this study is to explore the molecular mechanism of IRF7 regulation on RPS18 transcription in M1‐type macrophages in pancreatic adenocarcinoma (PAAD) tissue, as well as the transfer of RPS18 by IRF7 via exosomes to PAAD cells and the regulation of ILF3 expression. Methods: By utilising single‐cell RNA sequencing (scRNA‐seq) data and spatial transcriptomics (ST) data from the Gene Expression Omnibus database, we identified distinct cell types with significant expression differences in PAAD tissue. Among these cell types, we identified those closely associated with lipid metabolism. The differentially expressed genes within these cell types were analysed, and target genes relevant to prognosis were identified. Flow cytometry was employed to assess the expression levels of target genes in M1 and M2 macrophages. Cell lines with target gene knockout were constructed using CRISPR/Cas9 editing technology, and cell lines with target gene knockdown and overexpression were established using lentiviral vectors. Additionally, a co‐culture model of exosomes derived from M1 macrophages with PAAD cells was developed. The impact of M1 macrophage‐derived exosomes on the lipid metabolism of PAAD cells in the model was evaluated through metabolomics analysis. The effects of M1 macrophage‐derived exosomes on the viability, proliferation, division, migration and apoptosis of PAAD cells were assessed using MTT assay, flow cytometry, EdU assay, wound healing assay, Transwell assay and TUNEL staining. Furthermore, a mouse PAAD orthotopic implantation model was established, and bioluminescence imaging was utilised to assess the influence of M1 macrophage‐derived exosomes on the intratumoural formation capacity of PAAD cells, as well as measuring tumour weight and volume. The expression of proliferation‐associated proteins in tumour tissues was examined using immunohistochemistry. Results: Through combined analysis of scRNA‐seq and ST technologies, we discovered a close association between M1 macrophages in PAAD samples and lipid metabolism signals, as well as a negative correlation between M1 macrophages and cancer cells. The construction of a prognostic risk score model identified RPS18 and IRF7 as two prognostically relevant genes in M1 macrophages, exhibiting negative and positive correlations, respectively. Mechanistically, it was found that IRF7 in M1 macrophages can inhibit the transcription of RPS18, reducing the transfer of RPS18 to PAAD cells via exosomes, consequently affecting the expression of ILF3 in PAAD cells. IRF7/RPS18 in M1 macrophages can also suppress lipid metabolism, cell viability, proliferation, migration, invasion and intratumoural formation capacity of PAAD cells, while promoting cell apoptosis. Conclusion: Overexpression of IRF7 in M1 macrophages may inhibit RPS18 transcription, reduce the transfer of RPS18 from M1 macrophage‐derived exosomes to PAAD cells, thereby suppressing ILF3 expression in PAAD cells, inhibiting the lipid metabolism pathway, and curtailing the viability, proliferation, migration, invasion of PAAD cells, as well as enhancing cell apoptosis, ultimately inhibiting tumour formation in PAAD cells in vivo. Targeting IRF7/RPS18 in M1 macrophages could represent a promising immunotherapeutic approach for PAAD in the future. [ABSTRACT FROM AUTHOR]

Objective To observe the expression of fructose-bisphosphate aldolase C (ALDOC) in colon cancer tissues and cells and to explore its effect on biological behavior. Methods GEPIA database was used to analyze the expression level of ALDOC in colon cancer tissues. The expression of ALDOC mRNA and protein in colon cancer tissues and cancer cells SW620, SW480, HT-29 and HCT-116 were detected by RT-qPCR and immu-nohistochemistry, and the relationship between ALDOC mRNA expression and clinicopathological factors was analyzed. Colon cancer cell SW620 was divided into control group, NC inhibitor group and ALDOC inhibitor group. ALDOC inhibitor or negative control was transfected into SW620 cells by lentivirus transfection kit. MTT assay and monoclonal formation of cancer cells were used to detect cell proliferation. Scratch test was used to detect cell migration ability, and transwell chamber test to detect the invasion ability of cells. The expression of ALDOC mRNA was detected by RT-qPCR, and that of ALDOC and MMP-2/9 was detected by western blot. Results GEPIA database showed that the expression level of ALDOC in colon cancer tissues was higher than that in normal tissues (P < 0.05). Compared with those in adjacent tissues or FHC, ALDOC mRNA and protein in colon cancer tissues and cells SW620, SW480, HT-29 and HCT-116 were up-regulated, and ALDOC mRNA level was significantly correlated with tumor differentiation, TNM stage and lymph node metastasis (P < 0.05). Down-regulating ALDOC mRNA could down-regulate MMP-2 and MMP-9 protein levels, inhibit cell growth, and reduce the number of wound healing and invasive cells (P < 0.05). Conclusion ALDOC level is up-regulated in colon cancer tissues and cells, and it is closely related to the poor prognosis of colorectal cancer patients. ALDOC regulates the invasion and migration of colon cancer cells by regulating MMP-2/9. [ABSTRACT FROM AUTHOR]

Background: Anaplastic lymphoma kinase (ALK) is one of the major driver genes of non-small cell lung cancer (NSCLC). Several studies have shown that the efficacy of pemetrexed in ALK-positive lung cancer is controversial. The aim of this study is to explore the efficacy of pemetrexed-based chemotherapy in patients with ALK-positive and negative lung adenocarcinoma., Methods: The clinical data of 98 cases of epidermal growth factor receptor (EGFR), kirsten rat sarcoma viral oncogene (KRAS), V-rafmurine sarcoma viral oncogene homolog B1 (BRAF)-negative patients with advanced lung adenocarcinoma patients who diagnosed by histopathology from January 2015 to April 2016 in the First Affiliated Hospital of Zhengzhou University were collected. The relationships between ALK gene status, clinical characteristics and response and progression-free survival (PFS) were analyzed., Results: All of the 98 patients' ALK status were determined. ALK gene fracture fusion occured in 34 cases (34.7%), no fracture fusion in 64 cases (65.3%). All patients underwent first-line pemetrexed and platinum-based chemotherapy, the objective response rate (ORR) was 21.4% and the disease control rate (DCR) was 84.7%. The ORR and DCR of patients with ALK fracture fusion were higher than those without fracture fusion (41.2% vs 10.9%, χ2=23.389, P<0.001; 91.2% vs 81.3%, χ2=4.153, P=0.042), the difference was statistically significant. ALK gene status was not related to age, gender, smoking history and clinical stage. The median PFS of ALK-positive lung adenocarcinoma was 7.1 months (95%CI: 6.1-8.1) and negative was 4.7 months (95%CI: 3.818-5.582), and the difference was statistically significant (χ2=13.269, P<0.001). Cox multivariate analysis indicates that PFS of pemetrexed combined with platinum chemotherapy was independent of gender, age, smoking, staging and platinum. ALK gene fracture fusion is an independent factor affecting PFS (HR=0.392, 95%CI: 0.243-0.634, P<0.001)., Conclusions: ALK-positive lung adenocarcinoma patients with first-line pemetrexed-based chemotherapy have greater clinical benefit than ALK-negative patients.