Search

Abstract Meningeal metastasis (LM) is commonly seen in the advanced stages of cancer patients, often leading to a rapid decline in survival time and quality of life. Currently, there is still a lack of standardized treatments. Oncolytic viruses (OVs) are a class of emerging cancer therapeutics with the advantages of selectively replicating in cancer cells, delivering various eukaryotic transgenes, inducing immunogenic cell death, and promoting anti-tumor immunity. Some studies applying OVs intrathoracically or intraperitoneally for the treatment of malignant pleural and peritoneal effusions have shown promising therapeutic effects. If OVs could be applied to treat LM, it would bring significant survival benefits to patients with LM. In this review, we analyzed past research on the use of viruses to treat meningeal metastasis, summarized the efficacy and safety demonstrated by the research results, and analyzed the feasibility of oncolytic virus therapy for meningeal metastasis. We also summarized the existing data to provide guidance for the development of OVs that can be injected into the cerebrospinal fluid (CSF).

Abstract The mechanisms underlying the natural control of hepatitis B virus (HBV) infection have long been an intriguing question. Given the wide physiological range of liver stiffness and the growing attention to the role of mechanical microenvironment in homeostasis and diseases, we investigated how physical matrix cues impact HBV replication. High matrix stiffness significantly inhibited HBV replication and activated YAP in primary hepatocyte culture system, a key molecule in mechanosignaling. YAP activation notably suppressed HBV transcription and antigen expression. Several YAP-induced genes exhibited strong anti-HBV effects. Single-cell analysis of liver tissue from male individuals with active HBV replication revealed a strong significant negative correlation between YAP signature activation and HBV transcript levels. Intraperitoneal administration of YAP small molecule agonist potently controls HBV in male mouse models. These findings unveil a mechanism that involves the mechanical environment of hepatocytes and YAP to clear hepatotropic viral infection in the liver, providing new perspectives for HBV cure studies and antiviral development.

Background: The brominated flame retardant 2, 2′, 4, 4′-tetrabromodiphenyl ether (PBDE-47) is well known as a developmental neurotoxicant, yet the underlying mechanisms remain elusive. Increasing evidence has demonstrated that brain glucose metabolism perturbation plays a role in neural impairments. Nevertheless, whether this disturbance is involved in PBDE-47-induced neurotoxicity remains unknown. Objectives: To explore the impacts of perinatal PBDE-47 exposure on brain glucose metabolism, and its link to thyroid hormones (THs) levels as well as neurobehavioral changes. Methods: Female Sprague-Dawley rats were orally exposed to PBDE-47 at environmentally relevant levels (0.1, 1.0, and 10.0 mg/kg bw) from pre-pregnancy through weaning of offspring. The male offspring were continued to raise to 88 days after birth for follow-up experiments. Morris water maze and Open field tests were performed to assess the neurobehavioral alterations. The brain glucose metabolism was evaluated using 18F-labeled fluorodeoxyglucose (18F-FDG) positron emission tomography. Serum THs levels were measured via enzyme-linked immunosorbent assay. Results: Perinatal exposure to PBDE-47 induced neurobehavioral impairments in adult male rats as evidenced by learning and memory impairments, hyperactivity and anxiety-like behavior. Moreover, positron emission tomography showed that the glucose metabolism in the whole and the specific brain regions were markedly declined. Interestingly, variations in brain glucose metabolism were associated with the increased serum triiodothyronine (T3) levels, and both were linked to neurobehavioral disorders. Conclusion: Exposure to environmentally related levels of PBDE-47 at critical developmental stages lowers glucose metabolism in the whole brain and in various brain regions, which is associated with behavioral and cognitive deficits in adult male rats. Moreover, the association may be influenced by the disturbance of T3 homeostasis.

Abstract Ultra-precision machining requires system modelling that both satisfies explainability and conforms to data fidelity. Existing modelling approaches, whether based on data-driven methods in present artificial intelligence (AI) or on first-principle knowledge, fall short of these qualities in high-demanding industrial applications. Therefore, this paper develops an explainable and generalizable ‘grey-box’ AI informatics method for real-world dynamic system modelling. Such a grey-box model serves as a multiscale ‘world model’ by integrating the first principles of the system in a white-box architecture with data-fitting black boxes for varying hyperparameters of the white box. The physical principles serve as an explainable global meta-structure of the real-world system driven by physical knowledge, while the black boxes enhance local fitting accuracy driven by training data. The grey-box model thus encapsulates implicit variables and relationships that a standalone white-box model or black-box model fails to capture. Case study on an industrial cleanroom high-precision temperature regulation system verifies that the grey-box method outperforms existing modelling methods and is suitable for varying operating conditions.

Abstract Purpose In this study, we aim to explore the efficacy of paxlovid on reducing mortality of COVID-19 patients in clinical setting, especially whether paxlovid modifies the risk of death in these severe and critical patients. Methods Our retrospective cohort study was conducted on the medical records of patients, consecutively admitted for COVID-19 to five hospitals in Chongqing, China from Dec 8, 2022 to Jan 20, 2023. Based on whether patients received paxlovid during their hospitalization, patients were grouped as paxlovid group and non-paxlovid group. We used 1:1 ratio propensity score matching (PSM) in our study to adjust for confounding factors and differences between groups. Statistical analysis were performed by SPSS 23.0. The differences in 28-day mortality between these two groups and its influencing factors were the main results we focused on. Results There were 1018 patients included in our study cohort. With 1:1 ratio PSM, each of the paxlovid group and non-paxlovid group included 237 patients. The results showed that patients using paxlovid have a lower 28-day mortality in overall population either before PSM (OR 0.594, 95% CI 0.385–0.917, p = 0.019) or after PSM (OR 0.458, 95% CI 0.272–0.774, p = 0.003) with multivariable adjusted logistic regression models. Meanwhile, in severe subgroup, it showed similar findings.With paxlovid treatment, it showed a significantly lower 28-day mortality in severe subgroup both before PSM (28% vs.41%, p = 0.008) and after PSM (19% vs.32%, p = 0.007). Conclusion Paxlovid can significantly reduce the risk of 28-day mortality in overall population and severe subgroup patients.This study distinguished the severe subgroup patients with COVID-19 who benefit more from paxlovid treatment.

Abstract Background Glioma is the most common primary brain tumor with high mortality and disability rates. Recent studies have highlighted the significant prognostic consequences of subtyping molecular pathological markers using tumor samples, such as IDH, 1p/19q, and TERT. However, the relative importance of individual markers or marker combinations in affecting patient survival remains unclear. Moreover, the high cost and reliance on postoperative tumor samples hinder the widespread use of these molecular markers in clinical practice, particularly during the preoperative period. We aim to identify the most prominent molecular biomarker combination that affects patient survival and develop a preoperative MRI-based predictive model and clinical scoring system for this combination. Methods A cohort dataset of 2,879 patients was compiled for survival risk stratification. In a subset of 238 patients, recursive partitioning analysis (RPA) was applied to create a survival subgroup framework based on molecular markers. We then collected MRI data and applied Visually Accessible Rembrandt Images (VASARI) features to construct predictive models and clinical scoring systems. Results The RPA delineated four survival groups primarily defined by the status of IDH and TERT mutations. Predictive models incorporating VASARI features and clinical data achieved AUC values of 0.85 for IDH and 0.82 for TERT mutations. Nomogram-based scoring systems were also formulated to facilitate clinical application. Conclusions The combination of IDH-TERT mutation status alone can identify the most distinct survival differences in glioma patients. The predictive model based on preoperative MRI features, supported by clinical assessments, offers a reliable method for early molecular mutation prediction and constitutes a valuable scoring tool for clinicians in guiding treatment strategies.

Objective We examined the factors influencing hospitalization and prognosis among patients with non-small cell lung cancer receiving epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) targeted therapy during the first wave of the coronavirus disease 2019 (COVID-19) pandemic. Methods In total, 267 patients diagnosed with NSCLC who were receiving treatment with third-generation EGFR-TKIs were included in our retrospective study. Data on patients’ demographics, clinical characteristics, and survival were collected and analyzed. Results Over a mean follow-up of 18 months, 80.5% (215/267) of the patients contracted COVID-19, and 12.6% (27/215) of these patients were hospitalized for COVID-19 treatment. Vaccinated patients, those with body mass index (BMI) ≥22.3 kg/m 2 , and those with no comorbidities had lower rates of infection and hospitalization than unvaccinated patients, those with BMI

BackgroundThis research aims to explore the intellectual landscape of studies in perioperative enteral nutrition (PEN) and identify trends and research frontiers in the field.MethodsScientometric research was conducted through the analysis of bibliographic records from the Web of Science Core Collection Database for the period 2014–2023. Analyses performed using CiteSpace software included cooperation network analysis, reference co-citation analysis, and keywords co-occurrence analysis.ResultsThe analysis included 3,671 valid records in the final dataset. Findings indicate an upward trend in annual publications, with the United States leading in research output and Harvard University as the top publishing institution. The Journal of Parenteral and Enteral Nutrition was identified as the most productive journal. Notable research hotspots include enhanced recovery after surgery, early enteral nutrition, intestinal failure, short bowel syndrome, abdominal surgery. Evidence-based articles have emerged as the predominant literature type. Future research trends are anticipated to focus on gut microbiota and patients with congenital heart disease.ConclusionOur study provides a comprehensive analysis of the publication volume, contributions by country/region and institutions, journal outlets, and reference and keyword clusters in the field of PEN over the decade. The findings provide valuable insights for researchers, policymakers, and clinicians, helping them comprehend the research landscape, identify gaps, and shape future research directions in this field.

Gravity energy storage is a type of energy storage method that utilizes gravitational potential energy to store energy. In recent years, it has been widely concerned by scholars and enterprises at home and abroad for its unique advantages. From the perspective of long-term profit, the economic analysis of the gravity energy storage system is essential. In previous studies, only some specific economic models are available for describing the gravity energy storage system. This article proposes a revenue model for the gravity energy storage system first. Then, suggest a method for operating and scheduling a decentralized slope-based gravity energy storage system based on peak valley electricity prices. This method aligns with the current business model of using user-side energy storage to participate in power system auxiliary services. Last, verify the feasibility of the process through analysis. This study will make a beneficial attempt for the market promotion and application of gravity energy storage.

Abstract Background Plasma microRNAs act as biomarkers for predicting and diagnosing diseases. Reliable non-invasive biomarkers for biochemical pregnancy loss have not been established. We aim to analyze the dynamic microRNA profiles during the peri-implantation period and investigate if plasma microRNAs could be non-invasive biomarkers predicting BPL. Methods In this study, we collected plasma samples from patients undergoing embryo transfer (ET) on ET day (ET0), 11 days after ET (ET11), and 14 days after ET (ET14). Patients were divided into the NP (negative pregnancy), BPL (biochemical pregnancy loss), and CP (clinical pregnancy) groups according to serum hCG levels at day11~14 and ultrasound at day28~35 following ET. MicroRNA profiles at different time-points were detected by miRNA-sequencing. We analyzed plasma microRNA signatures for BPL at the peri-implantation stage, we characterized the dynamic microRNA changes during the implantation period, constructed a microRNA co-expression network, and established predictive models for BPL. Finally, the sequencing results were confirmed by Taqman RT-qPCR. Results BPL patients have distinct plasma microRNA profiles compared to CP patients at multiple time-points during the peri-implantation period. Machine learning models revealed that plasma microRNAs could predict BPL. RT-qPCR confirmed that miR-181a-2-3p, miR-9-5p, miR-150-3p, miR-150-5p, and miR-98-5p, miR-363-3p were significantly differentially expressed between patients with different reproductive outcomes. Conclusion Our study highlights the non-invasive value of plasma microRNAs in predicting BPL.

Deformable wave energy converters have significant potential for application as flexible material that can mitigate structural issues, while how to design the dimensions and choose an optimal deployment location remain unclear. In this paper, fully coupled computational fluid dynamics and computational solid mechanics were used to simulate the dynamic interactions between ocean waves and a deformable wave energy converter. The simulation results showed that the relative length to wave, deployment depth and aspect ratio of the device have significant effects on the energy conversion efficiency. By calculating the energy captured per unit width of the device, the energy efficiency was found to be up to 138%. The optimal energy conversion efficiencies were achieved when the structure length was 0.25, 0.5 or 0.75 of the dominating wavelength and submerged at a corresponding suitable depth. The aspect ratio and maximum stress inside the wave energy converter showed a nonlinear trend, with potential optimal points revealed. The simulation approach and results support the future design and optimisation of flexiable wave energy converters or other marine structures with notable deformations.

Metal-organic framework (MOF) materials, characterized by their porosity and large specific surface areas, exhibit excellent adsorption properties. With the aim of removing Te(IV) from environmental aquatic systems, this study is the first to propose the use of MIL-100(Fe). The material reveals a strong adsorption capacity for Te(IV), with maximum adsorption of 531.9 mg/g, superior to other adsorbent materials. Adsorption isotherm and kinetic models indicate that the adsorption process primarily involves monolayer chemical adsorption. According to the thermodynamic parameters, the adsorption reaction is endothermic. The experiment individually examined factors affecting the material's adsorption performance, including adsorbent dose, initial concentration of Te(IV), pH, adsorption time, and coexisting ions. Even under high ion strength conditions and high concentrations of coexisting ions, the material's adsorption efficiency for Te(IV) still reached over 94%. The material has been successfully applied to remove Te(IV) from lake water, river water, and seawater, yielding satisfactory results. Due to the high salinity and ionic strength of the solution, the removal efficiency of Te(IV) in the seawater matrix was slightly lower than that in freshwater (river and lake water). Thus, this material shows promise for the removal of Te(IV) from complex aquatic systems. HIGHLIGHTS MIL-100(Fe) was prepared and characterized.; The Te(IV) can be adsorbed by MIL-100(Fe) effectively, and the adsorption process was primarily a monolayer chemical adsorption and an endothermic reaction.; The Te(IV) can be removed effectively in environmental water supplies by MIL-100(Fe).;

Abstract Background Lamiophlomis rotata (Benth.) Kudo (L. rotata), the oral Traditional Tibetan herbal medicine, is adopted for treating knife and gun wounds for a long time. As previously demonstrated, total iridoid glycoside extract of L. rotata (IGLR) induced polarization of M2 macrophage to speed up wound healing. In diabetic wounds, high levels inflammatory and chemotactic factors are usually related to high reactive oxygen species (ROS) levels. As a ROS target gene, nuclear factor erythroid 2-related factor 2 (NRF2), influences the differentiation of monocytes to M1/M2 macrophages. Fortunately, iridoid glycosides are naturally occurring active compounds that can be used as the oxygen radical scavenger. Nevertheless, the influence of IGLR in diabetic wound healing and its associated mechanism is largely unclear. Materials and methods With macrophages and dermal fibroblasts in vitro, as well as a thickness excision model of db/db mouse in vivo, the role of IGLR in diabetic wound healing and the probable mechanism of the action were investigated. Results Our results showed that IGLR suppressed oxidative distress and inflammation partly through the NRF2/cyclooxygenase2 (COX2) signaling pathway in vitro. The intercellular communication between macrophages and dermal fibroblasts was investigated by the conditioned medium (CM) of IGLR treatment cells. The CM increased the transcription and translation of collagen I (COL1A1) and alpha smooth muscle actin (α-SMA) within fibroblasts. With diabetic wound mice, the data demonstrated IGLR activated the NRF2/KEAP1 signaling and the downstream targets of the pathway, inhibited COX2/PEG2 signaling and decreased the interaction inflammatory targets of the axis, like interleukin-1beta (IL-1β), interleukin 6 (IL-6), apoptosis-associated speck-like protein (ASC), cysteinyl aspartate specific proteinase1 (caspase1) and NOD-like receptor-containing protein 3 (NLRP3).In addition, the deposition of COL1A1, and the level of α-SMA, and Transforming growth factor-β1 (TGF-β1) obviously elevated, whereas that of pro-inflammatory factors reduced in the diabetic wound tissue with IGLR treatment. Conclusion IGLR suppressed oxidative distress and inflammation mainly through NRF2/COX2 axis, thus promoting paracrine and accelerating wound healing in diabetes mice. Graphical Abstract

Abstract Background Ubiquitination is a critical post-translational modification which can be reversed with an enzyme family known as deubiquitinating enzymes (DUBs). It has been reported that dysregulation of deubiquitination leads to carcinogenesis. As a member of the DUBs family, proteasome 26 S subunit non-ATPase 7 (PSMD7) serves as an underlying tumour-promoting factor in multiple cancers. However, the clinical significance and biological functions of PSMD7 in pancreatic cancer (PC) remain unclear. Results In this study, we first reported frequent overexpression of PSMD7 in PC tissues, and high levels of PSMD7 were markedly linked to shorter survival and a malignant phenotype in PC patients. An array of in vitro and in vivo gain/loss-of-function tests revealed that PSMD7 facilitates the progression of PC cells. Additionally, we found that PSMD7 promotes PC cell progression by activating the Notch homolog 1 (Notch1) signalling. Interestingly, in PC cells, the inhibitory effect of PSMD7 knockdown on cellular processes was comparable to that observed upon Notch1 knockdown. Mechanistically, PSMD7 deubiquitinated and stabilised sex determining region Y (SRY)-box 2 (SOX2), a key mediator of Notch1 signalling. The stabilisation of SOX2, mediated by PSMD7, dramatically increased SOX2 protein levels, subsequently activating the Notch1 pathway. Finally, restoration of SOX2 expression abrogated the PSMD7-silenced antitumour effect. Conclusions Taken together, our work identifies and validates PSMD7 as a promoter of PC progression through augmentation of the Notch1 signalling pathway mediated by SOX2. This finding suggests that PSMD7 holds promise as a potential therapeutic target for the management of this refractory disease.

ABSTRACTWe report investigations of the magnetic textures in periodic multilayers [Pt(1 nm)/(CoFeB(0.8 nm)/Ru(1.4 nm)]10 using polarised neutron reflectometry (PNR) and small-angle neutron scattering (SANS). The multilayers are known to host skyrmions stabilized by Dzyaloshinskii-Moriya interactions induced by broken inversion symmetry and spin–orbit coupling at the asymmetric interfaces. From depth-dependent PNR measurements, we observed well-defined structural features and obtained the layer-resolved magnetization profiles. The in-plane magnetization of the CoFeB layers calculated from fitting of the PNR profiles is found to be in excellent agreement with magnetometry data. Using SANS as a bulk probe of the entire multilayer, we observe long-period magnetic stripe domains and skyrmion ensembles with full orientational disorder at room temperature. No sign of skyrmions is found below 250 K, which we suggest is due to an increase of an effective magnetic anisotropy in the CoFeB layer on cooling that suppresses skyrmion stability. Using polarised SANS at room temperature, we prove the existence of pure Néel-type windings in both stripe domain and skyrmion regimes. No Bloch-type winding admixture, i.e. an indication for hybrid windings, is detected within the measurement sensitivity, in good agreement with expectations according to our micromagnetic modelling of the multilayers. Our findings using neutron techniques provide valuable microscopic insights into the rich magnetic behavior of skyrmion-hosting multilayers, which are essential for the advancement of future skyrmion-based spintronic devices.

Abstract Background Glioma recurrence, subsequent to maximal safe resection, remains a pivotal challenge. This study aimed to identify key clinical predictors influencing recurrence and develop predictive models to enhance neurological diagnostics and therapeutic strategies. Methods This longitudinal cohort study with a substantial sample size (n = 2825) included patients with non-recurrent glioma who were pathologically diagnosed and had undergone initial surgical resection between 2010 and 2018. Logistic regression models and stratified Cox proportional hazards models were established with the top 15 clinical variables significantly influencing outcomes screened by the least absolute shrinkage and selection operator (LASSO) method. Preoperative and postoperative models predicting short-term (within 6 months) postoperative recurrence in glioma patients were developed to explore the risk factors associated with short- and long-term recurrence in glioma patients. Results Preoperative and postoperative logistic models predicting short-term recurrence had accuracies of 0.78 and 0.87, respectively. A range of biological and early symptomatic characteristics linked to short- and long-term recurrence have been pinpointed. Age, headache, muscle weakness, tumor location and Karnofsky score represented significant odd ratios (t > 2.65, p 4.12, p

Abstract Background Aphids often harbor bacterial symbionts that confer resistance to biotic and abiotic stress. Previous studies have primarily examined the direct effects of symbiont infection on parasitoid success but less on aphid population dynamics under indirect parasitic situations, for example when exposed to parasitoid wasp odor. Deciphering this type of indirect effect is essential for understanding dynamics of insect ecosystems and communities and to improve IPM success. Methods We generated Sitobion avenae aphid clonal lines that are genetically identical but differ in Regiella insecticola infection. Then, the indirect odor effect of female parasitoid wasp Aphidius gifuensis (Ashmaed), one of its major natural enemies in the fields, was examined on the aphid lines fitness using different parasitoid densities. With these lines we also tested the direct effect of symbiont presence on aphid resistance against different parasitoid densities. Results Our study found fitness costs for the aphid line hosting Regiella, mainly via an increase in the development time and a reduction in population increase rate. Some of these fitness traits were influenced by the indirect exposure to parasitoid wasp odor with a density effect. Presence of the symbiont also reduced A. gifuensis parasitic success, increased the wasp development time and decreased its emergence weight with low effect of the parasitoid density used for parasitism. Conclusions These results showed that aphid population dynamic was mainly affected by the symbiont presence, but not by parasitoid odor. Symbiont presence also protected aphid from parasitism and affected parasitoid offspring weight and hence their future individual fertility and fitness.

Melon (Cucumis melo) includes more than ten botanical groups, many of which feature netting ornamentation on the surface of mature fruit. Ripe melons display a netted skin that signifies their ripeness and readiness for consumption. Previously, we identified SKIN NETTING (CmSN), which encodes an EamA-like transporter family protein, as the candidate gene controlling fruit skin netting formation in melon, while its biological functions remain unclear. In this study, we demonstrated that the expression of the CmSN gene was considerably lower in netted melons compared to smooth-skinned melons, indicating a negative correlation between CmSN expression and netting formation. Subsequently, we employed transient overexpression and virus-induced gene silencing (VIGS) experiments to explore the role of CmSN gene during fruit development. Overexpression of the CmSN gene inhibited netting development, whereas silencing it promoted netting formation. Using heterologous transformation in tomato, we further confirmed the effect of the CmSN gene on rind texture and toughness, as these tomatoes exhibited rougher and tougher skins. Analysis with near-isogenic lines (NILs) revealed that CmSN gene-bearing fruits (NIL_CmSN) possessed significantly harder rinds than the control smooth-skinned variety HB42, underscoring the role of CmSN in enhancing rind protection. Together, our research offers essential insights into the netting formation and genetic improvement of melon fruits.

Surface acoustic waves (SAWs) are excited by femtosecond extreme ultraviolet (EUV) transient gratings (TGs) in a room-temperature ferrimagnetic DyCo5 alloy. TGs are generated by crossing a pair of EUV pulses from a free electron laser with the wavelength of 20.8 nm matching the Co M-edge, resulting in a SAW wavelength of Λ = 44 nm. Using the pump-probe transient grating scheme in reflection geometry, the excited SAWs could be followed in the time range of −10 to 100 ps in the thin film. Coherent generation of TGs by ultrafast EUV pulses allows to excite SAW in any material and to investigate their couplings to other dynamics, such as spin waves and orbital dynamics. In contrast, we encountered challenges in detecting electronic and magnetic signals, potentially due to the dominance of the larger SAW signal and the weakened reflection signal from underlying layers. A potential solution for the latter challenge involves employing soft x-ray probes, albeit introducing additional complexities associated with the required grazing incidence geometry.

Abstract Background The aim of this article is to establish an external quality assessment (EQA) scheme for sperm Deoxyribonucleic acid (DNA) fragmentation (SDF) detection, and to assess the feasibility of the scheme. In addition, this article provides some case analysis of abnormal results in order to really help improve the performance of the laboratory. Results In 2021 and 2022, 10 and 28 laboratories in China volunteered to participate in the EQA program respectively. Two samples were selected for EQA each year, a large spread of results was obtained for the four samples, and the highest values were 13.7, 4.2, 8.0 and 4.0 times the lowest respectively. The coefficients of variation (CVs) were very high for the four samples, at 46.6%, 30.1%, 26.7% and 30.3%, respectively. The CVs of the samples with high SDF values were lower than those of the samples with low SDF values. There was no significant difference between the results of sperm chromatin structure assay (SCSA) and sperm chromatin dispersion (SCD). For the 10 laboratories that participated in EQA in 2021 and 2022, the CVs of low SDF value samples and high SDF value samples decreased from 46.6% and 30.1% in 2021 to 32.5% and 22.7% in 2022, respectively. Conclusion This is the first study to evaluate the EQA program on SDF, which involved a number of laboratories and was demonstrated to be feasible. It is recommended that all laboratories participate in the EQA of SDF to ensure the accuracy of the results.

Abstract Background The basic leucine zipper (bZIP) transcription factor family is one of the most abundant and evolutionarily conserved gene families in plants. It assumes crucial functions in the life cycle of plants, including pathogen defense, secondary metabolism, stress response, seed maturation, and flower development. Although the genome of wax gourd has been published, little is known about the functions, evolutionary background, and gene expression patterns of the bZIP gene family, which limits its utilization. Results A total of 61 bZIP genes (BhbZIPs) were identified from wax gourd (Benincasa hispida) genome and divided into 12 subgroups. Whole-genome duplication (WGD) and dispersed duplication (DSD) were the main driving forces of bZIP gene family expansion in wax gourd, and this family may have undergone intense purifying selection pressure during the evolutionary process. We selected BhbZIP58, only one in the member of subgroup B, to study its expression patterns under different stresses, including heat, salt, drought, cold stress, and ABA treatment. Surprisingly, BhbZIP58 had a dramatic response under heat stress. BhbZIP58 showed the highest expression level in the root compared with leaves, stem, stamen, pistil, and ovary. In addition, BhbZIP58 protein was located in the nucleus and had transcriptional activation activity. Overexpression of BhbZIP58 in Arabidopsis enhanced their heat tolerance. Conclusions In this study, bZIP gene family is systematically bioinformatically in wax gourd for the first time. Particularly, BhbZIP58 may have an important role in heat stress. It will facilitate further research on the bZIP gene family regarding their evolutionary history and biological functions.

Background: The coexistence of Chronic hepatitis B (CHB) and non-alcoholic fatty liver disease (NAFLD) is becoming more prevalent, which not only exacerbates the original hepatitis symptoms, but also boosts the likelihood of cirrhosis and hepatocellular cancer (HCC). Nevertheless, the common mechanism of its occurrence is still obscure, the purpose of this study is to discover the shared pathogenesis. Methods: The Gene Expression Omnibus (GEO) database was used to get the gene expression data sets for CHB (GSE83148) and NAFLD (GSE89632). Following the identification of the common differentially expressed genes (DEGs), functional annotation was carried out. Moreover, protein-protein interaction (PPI) network and key sub-network were constructed. Finally, the hub gene was discovered and their transcription factors (TFs) analysis were performed. Results: In the subsequent analyses, 54 common DEGs were selected. Functional analysis highlights the crucial role of immunity in these two diseases. Furthermore, IL2RB, GZMH, NKG7, CD2, CD48, TCF19, CXCL9, CCNA2, GZMA, CD8A, TNFAIP8L2, CD3D, TYMS, PRF1 and CCL5 were identified as significant hub genes, as their TFs were SP1, TP53, and ESR1. Conclusion: Our findings suggest that an immune-related mechanism may be the core pathogenesis of CHB and NAFLD, the clue sheds light on further mechanism research.

Abstract Introduction N‐acetylcysteine (NAC) prevents acute exacerbations of chronic obstructive pulmonary disease (AECOPD). However, the value of NAC inhalation in the treatment of patients with AECOPD is still poorly understood. The study was conducted to evaluate the efficacy of NAC inhalation in AECOPD patients requiring hospitalization. Methods In this single institutional, retrospective cohort study, all patients with AECOPD requiring hospitalization between January 2021 and January 2022 were included. Patients were divided into NAC group and Non‐NAC group according to whether being treated with NAC inhalation and were matched using the propensity score. The primary outcome was a composite of progression to ventilation requirement, in‐hospital mortality and readmission for AECOPD within 30 days. The effect on the mean hospitalized days, blood gas indexes and the incidence rate of adverse drug events were compared between the two groups. Results Ninety‐six patients in the NAC group were matched with 96 patients in the Non‐NAC group. The differences in the primary composite end point (NAC group vs Non‐NAC group, 5.2% vs 16.7%; P = 0.011) were significant. The median time to discharge was shorter in the NAC group (8.3 vs. 9.1 days, P = 0.030). The NAC group presented a larger increase in partial pressure of arterial oxygen (PaO2) and a higher ratio of self‐reported symptomatic improvement from admission to day 5. There was no definite difference between the two groups in the frequency of adverse event. Conclusion NAC inhalation is associated with an improved clinical outcome. A further study should be conducted to confirm the clinical usefulness of NAC inhalation in AECOPD patients.

Background and aims – Within angiosperms, vivipary has been reported in less than 0.1% of all species. We herein report the first occurrence of vivipary in an Afrotropical Melastomataceae and discuss its phylogenetic position, habit, habitat, and fruit attributes. Material and methods – Observational data were gathered from a field expedition to Gabon, and from herbarium specimens from BR, BRLU, P, and WAG. A phylogeny was generated to map all the eight species exhibiting vivipary in Melastomataceae. Key results – Amphiblemma ciliatum is currently the only known melastome species in the Afrotropics exhibiting vivipary. It is likely that its angular capsules depend on rainwater for seed dispersal. Conclusion – The combination of a herbaceous habit and angular fruits with the occurrence on shaded humid tropical rainforest floor during periods of incessant rainfall and high humidity might be responsible for vivipary in most Melastomataceae.

Abstract Isolated magnetic skyrmions are stable, topologically protected spin textures that are at the forefront of research interests today due to their potential applications in information technology. A distinct class of skyrmion hosts are rare earth - transition metal (RE-TM) ferrimagnetic materials. To date, the nature and the control of basic traits of skyrmions in these materials are not fully understood. We show that for an archetypal ferrimagnetic material DyCo3 that exhibits a strong perpendicular anisotropy, the ferrimagnetic skyrmion size can be tuned by an external magnetic field. Moreover, by taking advantage of the high spatial resolution of scanning transmission X-ray microscopy (STXM) and utilizing a large x-ray magnetic linear dichroism (XMLD) contrast that occurs naturally at the RE resonant edges, we resolve the nature of the magnetic domain walls of ferrimagnetic skyrmions. We demonstrate that through this method one can easily discriminate between Bloch and Néel type domain walls for each individual skyrmion. For all isolated ferrimagnetic skyrmions, we observe that the domain walls are of Néel-type. This key information is corroborated with results of micromagnetic simulations and allows us to conclude on the nature of the Dzyaloshinskii-Moriya interaction (DMI) which concurs to the stabilisation of skyrmions in this ferrimagnetic system. Establishing that an intrinsic DMI occurs in RE-TM materials will also be beneficial towards a deeper understanding of chiral spin texture control in ferrimagnetic materials.

Abstract The tomato pinworm, Tuta absoluta, or Phthorimaea absouta, is native to South America, but quickly spread to other regions of world, including Europe, Africa, and Asia, devastating to global tomato production. However, a lack of high-quality genome resources makes it difficult to understand its high invasiveness and ecological adaptation. Here, we sequenced the genome of the tomato pinworm using Nanopore platforms, yielding a genome assembly of 564.5 Mb with contig N50 of 3.33 Mb. BUSCO analysis demonstrated that this genome assembly has a high-level completeness of 98.0% gene coverage. In total, 310 Mb are repeating sequences accounting for 54.8% of genome assembly, and 21,979 protein-coding genes are annotated. Next, we used the Hi-C technique to anchor 295 contigs to 29 chromosomes, yielding a chromosome-level genome assembly with a scaffold N50 of 20.7 Mb. In sum, the high-quality genome assembly of the tomato pinworm is a useful gene resource that contributes to a better understanding of the biological characteristics of its invasiveness and will help in developing an efficient control policy.

The burgeoning dockless bike-sharing system presents a promising solution to the first- and last-mile transportation challenge by connecting trip origins/destinations to metro stations. However, the differentiation between metro passengers and DBS riders, as they belong to distinct systems, hinders the precise identification of DBS-metro transfers. This study introduces an innovative method employing mobility chains to establish spatiotemporal relationships, including spatiotemporal conflicts and similarities, among potential users from both systems. This significantly enhances the precision of user matching. An empirical study in Chengdu validates the method’s increased accuracy and examines travel patterns, yielding the following insights: (1) Introduction of the mobility chain reduces average matched pairs by 28.27% and improves accuracy by 18.36%. The addition of spatial-temporal similarity further boosts accuracy by 19.32%. (2) Median distances for DBS-metro access and egress transfers are approximately 950 meters. Short trips of 650–750 meters are prevalent, while trips exceeding 1.5 kilometers lead passengers to opt for alternative modes. (3) Temporal patterns reveal weekday peaks at 8:00, 9:00, and 17:00. On weekends, transfers are uniformly distributed, mainly within urban areas. Suburban stations exhibit reduced weekend activity. These findings can provide valuable insights for enhancing DBS bicycle redistribution, promoting transportation mode integration, and fostering urban transportation’s sustainable development.

Regenerating family protein 3 A (Reg3A) is highly expressed in a variety of organs and inflammatory tissues, and is closely related to tumorigenesis and cancer progression. However, clinical statistics show that high expression of Reg3A is associated with better prognosis in colorectal cancer (CRC) patients, suggesting a tumor-suppressive effect. The precise action and underlying mechanism of Reg3A in CRC remain controversial. The present study sought to investigate the relationship among Reg3A expression, CRC development, and immune cell alteration in patients using the TCGA, GEPIA, PrognoScan, TIMER and TISIDB databases. Reg3A-overexpressing LoVo cell line (LoVo-Reg3A), a representative of colon adenocarcinoma (COAD), was constructed and the action of Reg3A was assessed in a xenograft nude mouse model. Our bioinformatical analyses revealed that Reg3A upregulation is highly associated with CRC, along with increased frequency of immune cell infiltration. In the xenograft nude mice, Reg3A overexpression offered a tumor-suppressive effect by inhibiting cell proliferation and promoting apoptosis. The result of RNA-seq suggested a positive regulation of leukocytes and an upregulation of T cells in LoVo-Reg3A tumor tissue. CD4+ and CD8+ T cells in tumors, splenic Reg3A-reactive IFN-γ+/CD4+ T cells, and serum TNF-α, IFN-γ and IL-17 were significantly increased by Reg3A overexpression. In the ex vivo co-culture experiment, elevated cytotoxic effect, increased proportion of CD3ε+ T cells, and upregulated expressions of TNF-α, IFN-γ and IL-17 were detected in the PBMCs isolated from LoVo-Reg3A cell-xenografted nude mice. In conclusion, high expression of Reg3A could activate and recruit T cells in COAD leading to the cytotoxic tumor-suppressive effect.

Abstract Supercapacitors have been used in a broad range of fields including electronics, transportation, and energies. Electrode materials with high capacitance and good rate performance are crucial for the future development and application of supercapacitors. Herein, we prepared N, O co‐doped defective carbon blocks (NO‐DCBs) with abundant active sites through carbonization and ball‐milling of polyimide. The as‐obtained NO‐DCBs exhibit high atomic content of N and O inherited from polyamic acid (PAA) precursor, as well as large amounts of intrinsic defects introduced by ball‐milling. Benefiting from the synergy of pseudocapacitance and electrical double‐layer capacitance provided by heteroatoms and intrinsic defects respectively, the NO‐DCBs assembled symmetric aqueous capacitor shows high capacitance of 329 F g−1 at 0.1 A g−1, good rate performance of 48% capacitance retention at 50 A g−1, and superb cycling stability. This work promotes the deep understanding of the synergy effect of functional groups and intrinsic defects for capacitive energy storage, and broadens the avenue for structural design of active sites in carbon materials.

Abstract Background Female infertility is a major problem for women of reproductive-age worldwide. Oxidative stress and inflammation are involved in processes related to female infertility. Serum uric acid levels, an indicator of oxidative stress and inflammation, have rarely been reported to be associated with female infertility. This study aimed to investigate the relationship between serum uric acid levels and female infertility. Methods This cross-sectional study included women aged 18–44 years from the National Health and Nutrition Examination Survey (NHANES) between 2013 and 2018. All data were extracted from NHANES questionnaires and laboratory measurements. Weighted univariable and multivariable logistic regression analyses were utilized to explore the relationship between serum uric acid and female infertility. Stratified analyses were performed based on body mass index (BMI, 30 years). The odds ratio (OR) with 95% confidence interval (CI) was used to report associations. Results A total of 2,884 women were included, of which 352 (13.30%) had infertility. Women with high serum uric acid concentrations were related to higher odds of infertility (OR = 1.20, 95%CI: 1.03–1.39) after adjusting for confounders. Compared with serum uric acid concentrations ≤ 3.72 mg/dL, women with uric acid concentrations of 4.43–5.13 mg/dL (OR = 1.65, 95%CI: 1.02–2.67) and > 5.13 mg/dL (OR = 1.86, 95%CI: 1.10–3.13) were related to higher odds of infertility. Stratified analyses showed that high serum uric acid concentrations were associated with higher odds of infertility in women with a BMI 30 years (OR = 1.23, 95%CI: 1.04–1.45), but not in women aged ≤ 30 years (P = 0.556). Conclusion Women with high serum uric acid concentrations were associated with higher odds of infertility, and this association may vary by BMI and age.

Abstract Lead halide perovskites are new key materials in various application areas such as high efficiency photovoltaics, lighting, and photodetectors. Doping with Mn, which is known to enhance the stability, has recently been reported to lead to ferromagnetism below 25 K in methylammonium lead iodide (MAPbI3) mediated by superexchange. Two most recent reports confirm ferromagnetism up to room temperature but mediated by double exchange between Mn2+ and Mn3+ ions. Here we investigate a wide concentration range of MAMn x Pb1−x I3 and Mn-doped triple-cation thin films by soft X-ray absorption, X-ray magnetic circular dichroism, and quantum interference device magnetometry. The X-ray absorption lineshape shows clearly an almost pure Mn2+ configuration, confirmed by a sum-rule analysis of the dichroism spectra. A remanent magnetization is not observed down to 2 K. Curie-Weiss fits to the magnetization yield negative Curie temperatures. All data show consistently that significant double exchange and ferromagnetism do not occur. Our results show that Mn is not suitable for creating ferromagnetism in lead halide perovskites.

As a newly conceived vibration isolation element, an inerter can be implemented in different forms, and it is easily introduced in different vibration isolation scenarios. This paper focuses on a novel inerter device called an electromagnetic inerter (EMI), which combines a linear generator with a fluid inerter. Firstly, the structure and the working principle of the EMI is stated. Then, the parameter sensitivity of the fluid inerter is analyzed, and two parameters that have great influence on the inertance coefficient are obtained. Subsequently, the influence of the change of the external circuit on the output characteristics of the device is also discussed. This proves that the introduction of external circuits can simplify complex mechanical topologies. Finally, the topological structures of vehicle suspension are changed in the form of an EMI (including external circuit), and the dynamic performance of these structures in the typical vibration isolation system of a vehicle’s suspension is obtained. It is found that an L4 layout should be considered as the best suspension structure. Compared with traditional passive suspension, it not only ensures that its handling stability is not weakened, but also reduces the root mean square value of body acceleration and the peak of suspension work space by 4.56% and 11.62%, respectively.

Abstract We present multiPrime, a novel tool that automatically designs minimal primer sets for targeted next‐generation sequencing, tailored to specific microbiomes or genes. MultiPrime enhances primer coverage by designing primers with mismatch tolerance and ensures both high compatibility and specificity. We evaluated the performance of multiPrime using a data set of 43,016 sequences from eight viruses. Our results demonstrated that multiPrime outperformed conventional tools, and the primer set designed by multiPrime successfully amplified the target amplicons. Furthermore, we expanded the application of multiPrime to 30 types of viruses and validated the work efficacy of multiPrime‐designed primers in 80 clinical specimens. The subsequent sequencing outcomes from these primers indicated a sensitivity of 94% and a specificity of 89%.

Abstract Weak absorption contrast in biological tissues has hindered x‐ray computed tomography from accessing biological structures. Recently, grating‐based imaging has emerged as a promising solution to biological low‐contrast imaging, providing complementary and previously unavailable structural information of the specimen. Although it has been successfully applied to work with conventional x‐ray sources, grating‐based imaging is time‐consuming and requires a sophisticated experimental setup. In this work, we demonstrate that a deep convolutional neural network trained with a generative adversarial network can directly convert x‐ray absorption images into differential phase‐contrast and dark‐field images that are comparable to those obtained at both a synchrotron beamline and a laboratory facility. By smearing back all of the virtual projections, high‐quality tomographic images of biological test specimens deliver the differential phase‐contrast‐ and dark‐field‐like contrast and quantitative information, broadening the horizon of x‐ray image contrast generation.

Summary: Defects are prevalent in two-dimensional (2D) materials due to thermal equilibrium and processing kinetics. The presence of various defect types can affect material properties significantly. With the development of the advanced transmission electron microscopy (TEM), the property-related structures could be investigated in multiple dimensions. It produces TEM datasets containing a large amount of information. Traditional data analysis is influenced by the subjectivity of researchers, and manual analysis is inefficient and imprecise. Recent developments in deep learning provide robust methods for the quantitative identification of defects in 2D materials efficiently and precisely. Taking advantage of big data, it breaks the limitations of TEM as a local characterization tool, making TEM an intelligent macroscopic analysis method. In this review, the recent developments in the TEM data analysis of defects in 2D materials using deep learning technology are summarized. Initially, an in-depth examination of the distinctions between TEM and natural images is presented. Subsequently, a comprehensive exploration of TEM data analysis ensues, encompassing denoising, point defects, line defects, planar defects, quantitative analysis, and applications. Furthermore, an exhaustive assessment of the significant obstacles encountered in the accurate identification of distinct structures is also provided.

The Chemokine-like factor (CKLF)-like MARVEL transmembrane domain-containing (CMTM) family, comprising nine members, is involved in the tumorigenesis and progression of various cancers. However, the expression profiles and clinical significance of CMTM family members in hepatocellular carcinoma (HCC) are not fully clarified. In this study, the RNA-sequencing and clinical data were downloaded from The Cancer Genome Atlas (TCGA) databases. The Kaplan–Meier method and the Cox proportional hazards regression analysis were used to evaluate the prognostic significance of CMTM family members. Single-sample gene set enrichment analysis (ssGSEA) and ESTIMATE algorithms were employed to explore the relationship between CMTM family genes and the tumor microenvironment in HCC. Finally, the prognostic CMTM family gene expression was further validated by quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical (IHC) staining in clinical HCC tissue specimens. The results indicated that, compared with normal tissues, the expression of CKLF, CMTM1, CMTM3, CMTM4, CMTM7, and CMTM8 were significantly upregulated in HCC, while the expression of CMTM2, CMTM5, and CMTM6 were significantly downregulated in HCC. Univariate and multivariate Cox regression analysis demonstrated that CKLF was an independent prognostic biomarker for the overall survival (OS) of HCC patients. In HCC, the expression of CKLF was found to be correlated with immune cell infiltration, immune-related functions, and immune checkpoint genes. The qRT-PCR and IHC confirmed that CKLF was highly expressed in HCC. Overall, this research suggested that CKLF is involved in immune cell infiltration and may serve as a critical prognostic biomarker, which provides new light on the therapeutics for HCC.

The cone-beam CT (CBCT) system based on the two-dimensional flat-panel detector technology is widely applied in patient location verification before radiotherapy. However, during the application of intraperitoneal tumor radiotherapy, severe shading and streaking artifacts caused by respiratory movement and intestinal peristalsis make it difficult to distinguish tumor areas from the CBCT images. Due to the non-rigid deformation of flexible organs such as the pancreas under the action of respiratory motion, it is hard to quantify deviation between the body surface motion monitoring results and the actual organ motion, and it is also difficult to monitor irregular motion represented by intestinal peristalsis. There is no effective solution to motion artifact correction in CBCT. Based on theory of biodynamics and common knowledge of human physiology, in this paper we propose a brand new radiotherapy image-guided cone-beam CT motion artifact correction method without motion monitoring or implantation of in-vivo markers. The proposed artifact correction strategy is designed based on the features of the artifact images and fusion of various CT image domain processing algorithms. The results suggest that the image quality of cone beam CT has been significantly improved after the application of this strategy in the clinical abdominal CBCT image processing. The average CT number error in typical soft tissue areas reduces from 90 HU to 30 HU, and the boundary of the intestinal cavity and surrounding soft tissue information are partially recovered. The proposed artifact correction strategy does not require respiratory gating or increase of projections, which can be integrated into existing workflows without marker implantation surgery. The motion-artifact-corrected CBCT images provide more accurate tumor localization information for image-guided radiotherapy of pancreatic carcinoma. The proposed method is proved practical and efficient for clinical applications

Digital tourism is an essential product of the digital economy, which is significant in promoting sustainable tourism. The study aims to analyze the current research status of digital tourism with the CiteSpace tool. One thousand thirteen documents related to digital tourism, AI tourism, and tourism digitization in the Web of Science (WOS) database from 1991–2022 were collected. This study conducts bibliometric analysis to portray the knowledge map. Furthermore, this paper explores the publication years, co-occurrence of authors, institutions, subjects, research hotspots, and evolution of digital tourism research. The analysis findings revealed that social and environmental sciences are the core disciplines in digital tourism research. Hong Kong Polytechnic University is the foremost institution, and Tussyadiah and Wirtz are the authors with the highest contribution rate. Moreover, the results emphasized the frontier studies of digital tourism referring to technology, consumption, tourists’ reviews, and the progress of the tourism industry. In addition, China, Europe, and the United States should strengthen their digital tourism cooperation with Africa and the South America to promote the sustainable development of global tourism. Finally, the results implicate the future development trends of digital tourism. AcknowledgmentWe acknowledge all the authors involved in the creation of this paper: Chen Luo for providing the opportunity for this research and collaborative creation, and Dr. Songyu Jiang, Dr. Ruihui Pu, and Dr. Lin Li for providing creative ideas and collaborative division for this paper, and for funding the research. Dr. Hongmei Yang further provides the research direction and future in the development process of digital tourism.

The advent of X-ray free-electron lasers (XFELs) has revolutionized fundamental science, from atomic to condensed matter physics, from chemistry to biology, giving researchers access to X-rays with unprecedented brightness, coherence and pulse duration. All XFEL facilities built until recently provided X-ray pulses at a relatively low repetition rate, with limited data statistics. Here, results from the first megahertz-repetition-rate X-ray scattering experiments at the Spectroscopy and Coherent Scattering (SCS) instrument of the European XFEL are presented. The experimental capabilities that the SCS instrument offers, resulting from the operation at megahertz repetition rates and the availability of the novel DSSC 2D imaging detector, are illustrated. Time-resolved magnetic X-ray scattering and holographic imaging experiments in solid state samples were chosen as representative, providing an ideal test-bed for operation at megahertz rates. Our results are relevant and applicable to any other non-destructive XFEL experiments in the soft X-ray range.

Abstract Background Meningioma is the most common type of primary intracranial tumor with 0.1–1% of all primary meningiomas have been reported to develop into metastases. However, there is no proven therapeutic strategy for multiple metastases of meningiomas. Case presentation A 60-year-old female accepted total tumor resection of a right frontal lobe meningioma in September 2018, In October 2021, the patient was admitted to hospital because of cough and shortness of breath and diagnosed with metastatic meningiomas. The computed tomography (CT) scan revealed the presence of large masses in the right thoracic and abdominal cavity. After two cycles of anti-PD-1 and anti-VEGF treatment, the symptoms were relieved and the tumor was necrotic. Follow up to June 21, 2022, the patient has been given eleven cycles of the treatment every 3 weeks without tumor progression. Conclusions This case showed combined anti-PD-1 and anti-VEGF treatment stimulates peripheral blood immune cells to kill metastatic meningioma cells. Whether combined immunotherapy is more effective for metastatic meningioma needs further exploration.

The helical magnetic structures of cubic chiral systems are well explained by the competition among Heisenberg exchange, Dzyaloshinskii-Moriya interaction, cubic anisotropy, and anisotropic exchange interaction (AEI). Recently, the role of the latter has been argued theoretically to be crucial for the low-temperature phase diagram of the cubic chiral magnet Cu_{2}OSeO_{3}, which features tilted conical and disordered skyrmion states for a specific orientation of the applied magnetic field (μ_{0}H[over ⃗]∥[001]). In this study, we exploit transmission resonant x-ray scattering in vector magnetic fields to directly quantify the strength of the AEI in Cu_{2}OSeO_{3} and measure its temperature dependence. We find that the AEI continuously increases below 50 K, resulting in a conical spiral pitch variation of 10% in the (001) plane. Our results contribute to establishing the interaction space that supports tilted cone and low-temperature skyrmion state formation, facilitating the goals for both a quantitative description and eventual design of the diverse spiral states existing amongst chiral magnets.

BackgroundPrevious studies have demonstrated that PANoptosis is strongly correlated with cancer immunity and progression. This study aimed to develop a PANoptosis-related signature (PANRS) to explore its potential value in predicting the prognosis and immunotherapy response of hepatocellular carcinoma (HCC).MethodsBased on the expression of PANoptosis-related genes, three molecular subtypes were identified. To construct a signature, the differentially expressed genes between different molecular subtypes were subjected to multivariate least absolute shrinkage and selection operator Cox regression analyses. The risk scores of patients in the training set were calculated using the signature. The patients were classified into high-risk and low-risk groups based on the median risk scores. The predictive performance of the signature was evaluated using Kaplan-Meier plotter, receiving operating characteristic curves, nomogram, and calibration curve. The results were validated using external datasets. Additionally, the correlation of the signature with the immune landscape and drug sensitivity was examined. Furthermore, the effect of LPCAT1 knockdown on HCC cell behavior was verified using in vitro experiments.ResultsThis study developed a PANRS. The risk score obtained by using the PANRS was an independent risk factor for the prognosis of patients with HCC and exhibited good prognostic predictive performance. The nomogram constructed based on the risk score and clinical information can accurately predicted the survival probability of patients with HCC. Patients with HCC in the high-risk groups have high immune scores and tend to generate an immunosuppressive microenvironment. They also exhibited a favorable response to immunotherapy, as evidenced by high tumor mutational burden, high immune checkpoint gene expression, high human leukocyte antigen gene expression, low tumor immune dysfunction and low exclusion scores. Additionally, the PANRS enabled the identification of 15 chemotherapeutic agents, including sorafenib, for patients with HCC with different risk levels, guiding clinical treatment. The signature gene LPCAT1 was upregulated in HCC cell lines. LPCAT1 knockdown markedly decreased HCC cell proliferation and migration.ConclusionPANRS can accurately predict the prognosis and immunotherapy response of patients with HCC and consequently guide individualized treatment.

Objective Drawing upon the health belief model, this study aims to analyze the message characteristics of coronavirus disease 2019 (COVID-19) vaccination promotion messages posted by influential Chinese public health institutions and how those characteristics affect audiences’ participative engagement on Weibo, which is a popular social media site in China. Methods Two Chinese phrases for the COVID-19 vaccine were adopted as search terms to retrieve qualified posts on Weibo from 1 December 2019 to 18 March 2023. A total of 2546 posts by the top nine most impactful public health institutions were retained for quantitative content analysis. Message characteristics derived from the health belief model and participative engagement indicators were coded by the authors. Results Among health belief model constructs, the collective-oriented constructs (i.e., benefits, cues to action, and susceptibility) appeared in almost half of the posts, while the individual-oriented constructs (i.e., barriers, self-efficacy, and severity) were mentioned less. Moreover, negative binomial regression models revealed that collective-oriented constructs and self-efficacy facilitated engagement, while other constructs played impeding roles. Conclusions Appearances and functions of the health belief model's constructs in the COVID-19 vaccination promotion context are closely associated with China's collectivistic culture. Furthermore, constructs conforming to people's psychological traits are likely to promote public engagement and may facilitate vaccination behavior.

The transient receptor potential channel (TRP channel) family is a kind of non- specific cation channel widely distributed in various tissues and organs of the human body, including the respiratory system, cardiovascular system, immune system, etc. It has been reported that various TRP channels are expressed in mammalian macrophages. TRP channels may be involved in various signaling pathways in the development of various systemic diseases through changes in intracellular concentrations of cations such as calcium and magnesium. These TRP channels may also intermingle with macrophage activation signals to jointly regulate the occurrence and development of diseases. Here, we summarize recent findings on the expression and function of TRP channels in macrophages and discuss their role as modulators of macrophage activation and function. As research on TRP channels in health and disease progresses, it is anticipated that positive or negative modulators of TRP channels for treating specific diseases may be promising therapeutic options for the prevention and/or treatment of disease.

ABSTRACT When it comes to the prevention of clinical signs and mortality associated with infection of the Newcastle disease virus (NDV), vaccination has been very effective. However, recent evidence has proven that more highly virulent strains are emerging that bypass existing immune protection and pose a serious threat to the global poultry industry. Here, a novel rescued adenovirus 5-coexpressed chicken granulocyte monocyte colony-stimulating factor (ChGM-CSF) bio-adjuvant and C22-hemagglutinin-neuraminidase (HN) boosted chickens’ immunological genetic resistance and thus improved the immunological effectiveness of the critical new-generation vaccine in vitro and in vivo. Accordingly, the hemagglutination inhibition (HI) titers (log2) of the recombinant adenovirus (rAdv)-ChGM-CSF-HN-immunized chickens had greater, more persistent, and longer-lasting NDV-specific antibodies than the La Sota and rAdv-HN-inoculated birds. Moreover, humoral and adaptive immunological conditions were shown to be in harmony after rAdv-ChGM-CSF-HN inoculation and uniformly enhanced the expression of alpha interferon (IFN-α), IFN-β, IFN-γ, interleukin-1β (IL-1β), IL-2, IL-16, IL-18, and IL-22. Postchallenge, the control challenge (CC), wild-type adenovirus (wtAdv), and rAdv-ChGM-CSF groups developed unique NDV clinical manifestations, significant viral shedding, high tissue viral loads, gross and microscopic lesions, and 100% mortality within 7 days. The La Sota, rAdv-HN, and rAdv-ChGM-CSF-HN groups were healthy and had 100% survival rates. The rAdv-ChGM-CSF-HN group swiftly regulated and stopped viral shedding and had lower tissue viral loads than all groups at 5 days postchallenge (dpc). Thus, the antiviral activity of ChGM-CSF offered robust immune protection in the face of challenge and reduced viral replication convincingly. Our advance innovation concepts, combining ChGM-CSF with a field-circulating strain epitope, could lead to the development of a safe, genotype-matched, universal transgenic vaccine that could eradicate the disease globally, reducing poverty and food insecurity. IMPORTANCE We studied the biological characterization of the developed functional synthetic recombinant adenoviruses, which showed a high degree of safety, thermostability, and genetic stability for up to 20 passages. It was demonstrated through both in vitro and in vivo testing that the immunogenicity of the proposed vaccine, which uses the T2A peptide from the Thosea asigna virus capsid protein supported by glycine and serine, helps with efficiency to generate a multicistronic vector, enables expression of two functional proteins in rAdv-ChGM-CSF-HN, and is superior to that of comparable vaccines. Additionally, adenovirus can be used to produce vaccines matching the virulent field-circulating strain epitope. Because there is no preexisting human adenoviral immunity detected in animals, the potency of adenoviral vaccines looks promising. Also, it ensures that the living vector does not carry the resistance gene that codes for the kanamycin antibiotic. Accordingly, a human recombinant adenoviral vaccine that has undergone biological improvements is beneficial and important.

It is urgent to solve the gender issues in global cross-cultural communication. Countries worldwide should responsible for achieving gender equality (SDG5). Hence, the study aims to portray the knowledge map of the gender issue in intercultural communication to explore the research status and future potential. The study used CiteSpace to conduct a bibliometric method within 2728 English articles on cross-cultural communication and gender equality topics from the Web of Science (WoS). After cluster analysis and time series analysis, this study emphasis the continued attention and increasing trend of publications and elaborates on the critical authors, institutions, and countries of research on this issue. The results introduced Putnick as the dominant author contributed to the topic. The University of Oxford ranked the top1 in the institution cooperation relationship. Europe countries and the United States have made major contributions and influenced Asian and African countries, such as Burkina Faso, North Macedonia, and Kosovo. Gender issues in Asia and Africa are getting much attention. The keyword clusters formed by the authors' cooperation include gender equality, life satisfaction, network analysis, and alcohol use. In addition, childbirth technology, patient safety competition, life satisfaction, capital safety, and sex difference are the key word clustering results of institutional cooperation. At the level of national cooperation, internet addition, risk sexual behavior, covid-19 pandemic and suicidal idea have become the main keywords The results of keyword cluster analysis show that gender role attribute, psychological properties, dating policy, professional fulfillment, and entrepreneurial intention have become the main topics in the current research. The research frontier analysis reflects the importance of gender, women and health. The research on self-efficacy, diversity, image, life satisfaction and choice has become the trend of cross-cultural communication and gender issues. Furthermore, abundant achievement emerged in the subjects of Psychology, Education, Sociology, and Business economics. Geography, Language and Literature, Medicine, and Health industries also have been highly influential in recent years. Therefore, the conclusion suggests the studies of gender issues can be further deepened into more authors, areas, subject and other multiple cooperation sectors.

In 2017, China introduced the Pilot Zones for Green Finance Reform and Innovations to achieve sustainable and high-quality economic growth. While this policy aims to promote the green transition of businesses and has drawn significant attention, its micro-operational mechanism and firm-level impact remain largely unexplored. This study addresses this research gap by employing a quasi-experimental approach to examine the policy’s effects on companies. The empirical results of our research highlight an unexpected 5.54% surge in the PM _2.5 concentration levels in the vicinity of these firms. Given these findings, we call on policymakers to reflect on the environmental consequences following the roll-out of the Green Finance Reform. Simultaneously, we stress the significance of corporate responsibility in disclosing pertinent indicators and environmental data.

This research employs China’s steel restriction policy as a backdrop to investigate environmental policies’ unintended and counterproductive effects. Using high-resolution satellite-derived data and panel Difference-in-Differences regression, we found that the air pollution concentration in cities implementing the steel restriction policy is 5.688 μ g/m ^3 higher than in control group cities. Additionally, the growth rate of air pollution in these cities is 6.577% faster. This quantitative evidence substantiates the backfire effect of the anticipation of environmental policy, where the delay between a policy’s announcement and its enforcement leads to a short-term surge in pollution levels. For China and other emerging economies, the development of a thorough and deliberate intergovernmental cooperation strategy is critical when formulating environmental policies. It involves synchronizing the efforts of different government levels in applying pollution controls and diminishing the interval of potential intense pollution in the pre-implementation phase.

Background and ObjectivesPatients with primary malignant brain tumors may experience mental health disturbances that can significantly affect their daily life. This study aims to identify risk factors and generate predictive models for postoperative mental health disturbances (PMHDs) in adult glioma patients in accordance with different clinical periods; additionally, survival analyses will be performed.MethodsThis longitudinal cohort study included 2,243 adult patients (age at diagnosis ≥ 18 years) with nonrecurrent glioma who were pathologically diagnosed and had undergone initial surgical resection. Six indicators of distress, sadness, fear, irritability, mood and enjoyment of life, ranging from 0-10, were selected to assess PMHDs in glioma patients in the third month after surgery, mainly referring to the M.D. Anderson Symptom Inventory Brain Tumor Module (MDASI-BT). Factor analysis (FA) was applied on these indicators to divide participants into PMHD and control groups based on composite factor scores. Survival analyses were performed, and separate logistic regression models were formulated for preoperative and postoperative factors predicting PMHDs.ResultsA total of 2,243 adult glioma patients were included in this study. Based on factor analysis results, 300 glioma patients had PMHDs in the third postoperative month, and the remaining 1,943 were controls. Candidate predictors for PMHDs in the preoperative model were associated with age, clinical symptoms (intracranial space-occupying lesion, muscle weakness and memory deterioration), and tumor location (corpus callosum, basal ganglia and brainstem), whereas age, clinical symptoms (nausea and memory deterioration), tumor location (basal ganglia and brainstem), hospitalization days, WHO grade 4, postoperative chemotherapy or radiotherapy and postoperative Karnofsky Performance Scale (KPS) served as important factors in the postoperative model. In addition, the median overall survival (OS) time for glioma patients with PMHDs was 19 months, compared to 13 months for glioblastoma, IDH-wild type (GBM) patients with PMHDs.ConclusionThe risk factors for PMHDs were identified. These findings may provide new insights into predicting the probability of PMHD occurrence in glioma patients in addition to aiding effective early intervention and improving prognosis based on different clinical stages.

Objective To understand the memory phenotype and function of SARS-CoV-2 reactive CD4+ T cells in healthy individuals. Methods In this study, SARS-CoV-2-derived peptides were used to stimulate PBMC from participants.SARS-CoV-2 reactive memory T cells were detected by intracellular staining and flow cytometry, and memory phenotype analysis was performed.CBA was used to detect cytokine secretion after SARS-CoV-2-derived peptides stimulation to evaluate the function of SARS-CoV-2 reactive memory T cells. Results We found that SARS-CoV-2 reactive CD4+ memory T cells could be detected in 40% (6/15) healthy donors.Phenotypic analysis of memory showed that these T cells were mainly composed of central memory T cells(82.2%), and other memory cells accounted for 17.8%.Compared with negative control, IL-10 was significantly decreased after stimulation of SARS-CoV-2-derived peptides (P