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Abstract Mobile technology has significantly accelerated the rapid development of healthcare services. Despite the convenience brought by the proliferation of mobile health (mHealth) apps, the challenge of promoting their continued use among patients has garnered attention from many scholars and administrators. Based on the Expectation Confirmation Model (ECM), this study explores the impact of quality elements on the continuance intention of mHealth apps in Southwest China’s ethnic minority regions. Researchers conducted a structured questionnaire survey on 337 users of mHealth apps in these regions to measure their self-reported responses to seven constructs: information quality, system quality, service quality, perceived usefulness, confirmation, satisfaction, and continuance intention. The study uses the structural equation model-artificial neural network (SEM-ANN) approach to interpret the compensatory and non-linear relationships between predictors and continuance intention. The findings reveal that user satisfaction and perceived usefulness significantly predict the continuance intention to use mHealth apps. All other relationships were confirmed except for the non-significant relationships between service quality and confirmation, service quality and perceived usefulness, and system quality and perceived usefulness. Furthermore, based on the normalized importance obtained from the multilayer perceptron, the most critical predictors identified were satisfaction (100%), followed by information quality (70.2%), perceived usefulness (43.2%), system quality (25.1%), and confirmation (17.6%). Finally, this study presents theoretical and practical implications for the continuance intention towards mHealth apps in Southwest China’s ethnic minority regions.

As shale oil exploration and development intensifies， the experimental technologies for shale oil development have been continuously improved while inheriting the experimental technologies and methods of conventional reservoirs and shale gas reservoirs， and a set of experimental technology systems of reservoir physics and flow mechanisms has been initially formed， which provides strong technical support for geological evaluation of shale reservoirs， reservoir engineering optimization design， and enhanced oil recovery. The development status of six experimental technologies in China and abroad， including porosity， permeability， oil-water saturation， wettability， relative permeability， and imbibition， was systematically summarized. In view of the characteristics of continental shale oil， such as low maturity， strong heterogeneity， and well-developed lamina and micro-fractures， the measurement methods and principles， key influencing factors， control conditions of experiments， advantages and disadvantages and technical applicability of experiment methods were analyzed. The main problems， development trends， and research direction of these experimental technologies were put forward. The results show that ① unifying the cleaning agent and cleaning time of rock samples is the key to improving the comparability of different measurement methods of porosity， and nuclear magnetic resonance measurement of porosity is the technological development direction to meet the needs of rapid analysis in mining fields； ② the irregular and multi-directional permeability measurement technology capable of simulating formation pressure changes is the future technological development direction； ③ the alcohol soaking method and two-dimensional nuclear magnetic resonance （T1-T2） method are the technological development direction of the determination of shale oil-water saturation， but they still face the problem of the influence of clay bound water and adsorbed oil on the determination accuracy； ④ the droplet morphology method is one of the future development directions of measurement technology of shale reservoir wettability， which requires further research and unified standards in experimental methods and comprehensive evaluation methods for surface wetting and spontaneous imbibition processes； ⑤ the combination of experimental measurement and numerical method is an effective way to obtain the relative permeability of shale reservoirs， and the micro-oil and water metering， end-effect correction， as well as technologies and methods considering the time-varying pressure are the key directions； ⑥ pressure imbibition measurement method should be used to evaluate imbibition effect and optimize well kill time in elastic development after fracturing. Pressure imbibition measurement methods based on nuclear magnetic resonance T2 spectrum， stratification T2 spectrum， and T1-T2 spectrum will be one of the essential technological development directions.

This paper summarized the nursing of a patient with chronic kidney failure with Spleen-Kidney Qi deficiency and internal heat syndrome treated with Thunder-Fire moxibustion combined with auricular comprehensive treatment based on the Yin-Fire theory. A comprehensive nursing plan including health education, emotion regulation and exercise guidance were carried out during hospitalization. Given the coexistence of multiple diseases, the Thunder-Fire moxibustion combined with auricular comprehensive treatment based on the Yin-Fire theory was carried out to relieve the fatigue and improve the self-care ability of the patient. (本文总结运用雷火灸疗法联合耳部综合疗法治疗1例慢性肾衰竭脾肾气虚兼内热证患的护理经验。在疾病宣教、情志护理、运动指导等常规护理基础上, 基于“阴火学说”, 根据患者多病共存的状态, 采用雷火灸联合耳部综合疗法, 有效改善患者乏力症状, 自理能力明显改善。)

This paper summarizes nursing measures for a patient with gout of damp-heat stagnation syndrome combined with erosive gastritis treated with auricular acupoint combine with pricking and cupping therapy. In addition to routine nursing, individualized auricular acupoint treatment including auricular bloodletting and pressing combined with pricking and cupping therapy were carried out, aiming at relieving heat and detoxification at early stage of disease, promoting blood circulation, dredging collaterals and regulating qi at midterm and invigorating spleen and warming kidney Yang and end stage of treatment. The combined treatment using Traditional Chinese medicine technology is effective to relieve the pain, reduce the joint swelling, and improve the recovery of joint movement, as well as protecting the gastric mucosa of the patient. (本文总结耳穴疗法联合刺络拔罐治疗1例痛风伴糜烂性胃炎的护理经验。在常规护理基础上, 通过制定个体化的耳穴疗法(包括耳穴放血和耳穴压豆)和刺络拔罐治疗, 基于早期泄热排毒, 中期活血散瘀通络疏肝理气, 后期健脾益气、温补肾阳的思路, 以达到即时止痛、快速消除关节肿胀、恢复关节活动功能及保护胃黏膜的效果。)

Abstract [Objective] Invasion of Trifolium repens occupies the living space of turfgrasses and has been seriously threatening the quality and function of turfs. Examining the impact of T . repens invasion on the functional traits of Poa pratensis and turf quality is helpful for understanding the invasion mechanism of T . repens and for establishing and maintaining gramineous turfgrass. [Methods] Taking the T . repens-invaded P . pratensis turf as object, the sample survey method was employed to investigate the differences in the functional traits and turf quality of P . pratensis under different degrees of T . repens invasion (control, low invasion, moderate invasion, and heavy invasion), and to evaluate turf quality by the membership function method. [Results] (1) With increase in degree of T . repens invasion, the carbon content of P . pratensis organs and the cost of leaf construction, as well as the ratio of carbon to nitrogen, carbon to phosphorus, and nitrogen to phosphorus were gradually decreased, while the specific leaf area and nitrogen and phosphorus content in the organs were increased. (2) At the same invasion level, the carbon, nitrogen, and phosphorus contents in the organs of P . pratensis were higher in leaves than in roots and stems, and the carbon to nitrogen ratio and carbon to phosphorus ratio were higher in roots and stems than in leaves, while the nitrogen to phosphorus ratio was higher in stems and leaves than in roots. (3) The density, texture, greenness index, and uniformity of P . pratensis turf were decreased with increase in T . repens invasion, while the aboveground biomass and belowground biomass were increased. (4) The comprehensive evaluation by the membership function method showed that turf quality was the best without T . repens invasion but the worst under heavy T . repens invasion. Leaf carbon content had the greatest influence on turf quality. [Conclusion] T . repens invasion alters P . pratensis functional traits as well as affects turf quality, landscape, and function.

Under the low-carbon development goal, energy storage allocation is the key measure to ensure the safe and economic operation of low-carbon parks, and to reduce carbon emissions. To solve the problems of inaccurate carbon emission calculation and insufficient utilization of equivalent energy storage resources in low-carbon parks, this paper proposes a dynamic emission factor calculation method based on the carbon emission flow theory, which realizes the accurate measurement of indirect carbon emissions from park electricity consumption. Then, taking into account the available equivalent energy storage resources in the park, it proposes an energy storage capacity optimization allocation model considering the equivalent energy storage characteristics of thermal system, and uses the big M method to equivalently transform the nonlinear constraints in the model. Finally, it conducts simulation analysis based on a case system to verify the correctness and effectiveness of the proposed model.

Lung cancer is characterized by high incidence and mortality rates and invasiveness, and its occurrence and development are influenced by various factors. Mitochondria, as ubiquitous organelles in the human body, regulate cellular processes, such as metabolism, signal transduction, oxidative stress, and genomic instability, thereby affecting the initiation and progression of lung cancer. This article summarizes the recent research progress on mitochondrial-targeted drugs, mitochondrial transfer, and mitochondrial gene therapy for lung cancer treatment. This work also discusses the principles and prospects of mitochondrial therapy to provide new insights for lung cancer treatment.

Osteoarthritis is caused by the cartilage destruction of the bones of the joint surfaces and structures that produce synovium fluid. Osteoarthritis treatment includes the use of surgical methods and non-surgical or maintenance treatments including knee orthoses, medical insoles with external edges, use of physiotherapy techniques, exercise, weight loss in obese people, and teaching the principles of joint care. The main goal of treatment in osteoarthritis of the knee is to ameliorate physical function, decrease pain, and reduce the progression of the disease, through correcting the knee alignment and reducing the varus torque. Previous studies have indicated that medicinal plants and herbal nanoparticles (NPs) have the best anti-inflammatory effects. β-Cyclodextrin is a cyclic carbohydrate molecule that is used as a host to prepare inclusion complexes. In this study, the synthesis of nickel NPs is based on β-cyclodextrin (NiBCD NPs) for accelerating the osteoarthritis articular cartilage repair. The FT-IR and XRD techniques confirmed the formula of NiO for the NiBCD NPs. The FE-SEM imaging shows a non-spherical structure for NiBCD NPs with a size of less than 100 nm. In EDX, the signals at the energy levels of 8.3, 7.5, and 0.87 keV are assigned for the electron migration of Ni Kβ, Ni Kα, and Ni Lα. Furthermore, the signals for the elements of oxygen and carbon of BCD appeared at 0.52 and 0.28 keV. The effectiveness of NiBCD NPs in promoting chondrogenesis was examined in orthopedic experiments using primary cultured chondrocytes. Subsequently, we determined the functional restoration following NiBCD NPs’ transplantation in a knee osteoarthritis articular cartilage injury model. We conducted histological, PCR, and Western blot assays. In the immunological analysis, the levels of MMPs, IL-1β, TNF-α, and p-p65 expression were found to be reduced by NiBCD NPs. This reduction may be attributed to the regulation of cellular redox homeostasis through Nrf2. Furthermore, our findings demonstrated the positive impact of NiBCD NPs on stimulating chondrogenesis in vitro. Notably, the NiBCD NPs’ application accelerated the recovery of injury-induced dysfunction. Additionally, the presence of NiBCD NPs at the injury site suppressed abnormal fibrosis and angiogenesis. The histological assay revealed the chondrocytes’ proliferation and increased cartilage matrix synthesis in the NiBCD NPs’ presence.

Objective To investigate the efficacy of single drug treatment of dapagliflozin and combination of dapagliflozin and sitagliptin in the treatment of type 2 diabetes mellitus (T2DM). Methods Sixty-two patients with T2DM admitted to Luhe District Hospital of Chinese Medicine from January 2022 to December 2023 were selected as research subjects, and the patients were randomly divided into two groups, with 31 cases in each group. The control group only received treatment with dapagliflozin (10 mg/d oral), while the observation group received both dapagliflozin (10 mg/d oral) and sitagliptin (100 mg/d oral) treatment. Both groups were treated for 3 months. The post-treatment blood glucose control effects ［fasting plasma glucose (FPG) and glycated hemoglobin (HbA1c)］, blood glucose fluctuations, inflammatory markers ［C-reactive protein (CRP)］, and cardiac function ［left ventricular ejection fraction (LVEF)］ were compared between two groups. Results After 3 months of treatment, compared with before treatment, both groups showed a significant decrease in FPG and HbA1c after treatment (P＜0.05). Compared with the control group, FPG ［(6.96±0.87) mmol/L vs (7.91±0.96) mmol/L, t=4.083, P＜0.01］ and HbA1c ［(6.54±0.33)% vs (7.65±0.58)%, t=9.261, P＜0.01］ of patients in the observation group were significantly decreased, and the standard deviation of mean blood glucose (SDBG) and amplitude of postprandial blood glucose fluctuation (PPGE) were lower (P

Objective To investigate the difference of dentate gyrus (DG) neuronal activity between wakefulness and sleep state, and the characteristics of DG neuronal activity during hippocampal sharp wave ripple and θ oscillations. Methods Six 3-month-dd male wild-type C57BL/6 mice (22~25 g) were employed. The oscillation state of local field potential in the dorsal hippocampus and three-dimensional acceleration of the head and neck were used to determine the brain state which were classified into three types:wakefulness, non-rapid eye movement (NREM) sleep and rapid eye movement (REM) sleep. In vivo multi-electrode arrays were utilized to record the firing activity of DG neurons under distinct states. Results The DG neurons were divided into the granule cells, mossy cells and interneurons according to characteristics of spike rates and valley-to-peak spike waveform width. The firing rates of granule cells (n=75) in NREM sleep were significantly higher than those in either REM sleep (P < 0.05) or wakefulness (P < 0.05). The granule cells exhibited further elevated spike activity during sharp wave ripples during NREM sleep and θ oscillations during REM sleep (P < 0.05). The firing rates of mossy cells (n=27) in REM sleep were significantly higher than in either NREM sleep (P < 0.05) or wakefulness (P < 0.05), but no statistical difference was observed in firing rates between NREM sleep and wakefulness. Likewise, the mossy cells exhibited increased spike activity during sharp wave ripples in NREM sleep and θ oscillations in REM sleep (P < 0.05). The firing rates of interneurons (n=9) during REM sleep were significantly higher than that in NREM sleep (P < 0.05). However, there was no significant difference in spike activities of interneurons between NREM sleep and wakefulness. 67% of the DG interneurons increased their spike activities, whereas the remaining interneurons (33%) decreased their spike activities during sharp wave ripples. The spike activity of interneurons had greatest decline among 3 types of DG neurons (P < 0.05). Conclusion The granule cells and mossy cells in the DG area are significantly different between wakefulness and sleep states in the firing activities. The granule cells exhibit strongest firing activities during NREM sleep, whereas the mossy cells show strongest firing activities during REM sleep.

Objective: A new type of natural food preservative with pork liver as the main raw material was developed. Methods: Water-soluble porcine liver protein (WSLP), salt-soluble porcine liver protein (SSLP) and chitosan (CS) were combined to create edible composite membranes. The edible membrane components were then examined from a variety of perspectives using mechanical properties, Fourier transform infrared spectroscopy (FT-IR) and microstructure analysis. Utilized to preserve pork, and by contrasting the changes in the physical and chemical indices during the chilling process, the freshness retention capabilities of the composite film were investigated. Results: With the addition of WSLP and SSLP, the mechanical properties of edible film showed a trend of increasing firstly and then decreasing. The film was best when the amount was 40%, its film thickness was (0.267±0.001 43) and (0.264±0.001 21) mm, the tensile strength was (9.63±0.29) and (4.43±0.37) MPa, and the elongation at break was (58.37±0.90)% and (28.24±0.63)%, respectively, which were significantly different from CS film (P

The phenomenon of population aging has become an important issue affecting the sustainable economic and social development of China's countryside, but few studies exist within the academic community on the sustainable livelihoods of rural elderly farmers in areas of poverty alleviation. Based on a 2022 field survey in Guzhang County, Hunan Province, this study examined the sustainable livelihood pathways of elderly rural farmers in these areas through non-participant observations, semi-structured interviews, and grounded theory methods of textual analysis. Study findings show that in the face of rural hollowing out caused by the outflow of the young labor force due to rapid urbanization, a large number of rural elderly farmers in poverty alleviation areas are facing the livelihood dilemma of rural pension structure constraints and the rational choice of agriculture. The background for this situation involves the insufficiency of the original pension model and multiple welfare sources, a shortage of medical and health service facilities, and the transformation of intergenerational relations. Therefore, based on the advantages of rural social networks in poverty alleviation areas in the three active aging dimensions of health, participation, and guarantee, this paper presents a sustainable livelihood research paradigm in which the individual, rural, and socioeconomic networks and rural production-living-ecological space of elderly farmers in poverty alleviation areas coordinate with each other. The paper also introduces a logical framework for the sustainable livelihood of elderly farmers that is consistent with the goal of rural revitalization through the thrust of nearby urbanization. Specific sustainable livelihood paths integrating individual spaces include the following: 1) at the individual level, through the individual livelihood behavior and livelihood capital of elderly farmers dynamically adjusting to the corresponding sources of welfare and livelihood modes with changes in the life cycle, 2) at the field space level, through the sixth industry, integrated development of modern factors intervening in the establishment of rural market industrial support to cope with aging, and 3) at the territorial space level, according to the rural elderly . The social circle structure of rural households is used to reshape the living, production, and ecological aging life circle. This study analyzed the sustainable livelihood paths of rural elderly farmers in poverty alleviation areas from individual to spatial perspectives The findings provide a theoretical basis and practical references for the revitalization of rural people in these areas in the era of rapid aging.

Objective·To evaluate the effects of levothyroxine (L-T4) dose adjustment according to the level of thyroid stimulating hormone (TSH) on maternal and infant outcomes in the pregnant women with hypothyroidism by meta-analysis.Methods·China National Knowledge Infrastructure (CNKI), China Science and Technology Journal Database (VIP), Wanfang Data Knowledge Service Platform, PubMed, Cochrane Library and Embase were retrieved to collect all the controlled studies on the treatment of pregnant women with hypothyroidism by adjusting the dose of L-T4 according to TSH level from the establishment of the databases to April 9, 2022. The references were also traced. Literature screening, data extraction, and quality evaluation were performed independently by two researchers. Cochrane evaluation was used to evaluate the quality of the included literature. Outcome indicators included gestational hypertension, gestational diabetes, postpartum hemorrhage, delivery mode, preterm birth, fetal death, neonatal asphyxia, and low birth weight infants. RevMan 5.3 was used for meta-analysis.Result·A total of 1 268 articles were retrieved from 6 databases, and 8 were included in the study, including 4 Chinese articles and 4 English articles. The overall risk of study bias was at a moderate level. Compared with the control group, the OR of gestational diabetes risk was 0.61 (95%CI 0.44‒0.86, P=0.004) and the OR of fetal death risk was 0.38 (95%CI 0.18‒0.81, P=0.010) in the experimental group with L-T4 dose adjusted according to the TSH level of the pregnant women with hypothyroidism, which were both statistically significant. However, the treatment method of adjusting L-T4 dose did not affect the risks of vaginal delivery [OR=1.82 (95%CI 0.75‒4.40, P=0.180)], gestational hypertension [OR=0.77 (95%CI 0.53‒1.12, P=0.170)], postpartum hemorrhage [OR=1.20 (95%CI 0.50‒2.92, P=0.680)], preterm birth [OR=0.72 (95%CI 0.48‒1.06, P=0.100)], low birth weight infants [OR=1.00 (95%CI 0.65‒1.54, P=0.999)], or neonatal asphyxia [OR=0.50 (95%CI 0.20‒1.27, P=0.150)] significantly.Conclusion·Adjusting the L-T4 therapeutic dose according to the TSH level may help reduce the risks of gestational diabetes and fetal death in the pregnant women with hypothyroidism.

For a non-empty set XX, denote the full transformation semigroup on XX by T(X)T\left(X) and suppose that EE is an equivalence relation on XX. Evidently, TE∗(X)={α∈T(X)∣(x,y)∈Eif and only if(xα,yα)∈Efor allx,y∈X}{T}_{{E}^{\ast }}\left(X)=\left\{\alpha \in T\left(X)| \left(x,y)\in E\hspace{0.33em}\hspace{0.1em}\text{if and only if}\hspace{0.1em}\hspace{0.33em}\left(x\alpha ,y\alpha )\in E\hspace{0.33em}\hspace{0.1em}\text{for all}\hspace{0.1em}\hspace{0.33em}x,y\in X\right\} is a subsemigroup of T(X)T\left(X). In this article, we investigate Green relations, Green ∗\ast -relations and Green ∼ \sim -relations, various kinds of regularities, ℱ{\mathcal{ {\mathcal F} }}-abundant and G{\mathcal{G}}-abundant elements and left and right magnifying elements in TE∗(X){T}_{{E}^{\ast }}\left(X). More specifically, we first obtain the necessary and sufficient conditions under which ℒ{\mathcal{ {\mathcal L} }} (respectively, ℒ∗{{\mathcal{ {\mathcal L} }}}^{\ast }, ℒ˜\widetilde{{\mathcal{ {\mathcal L} }}}, ℛ{\mathcal{ {\mathcal R} }}, ℛ∗{{\mathcal{ {\mathcal R} }}}^{\ast }, and ℛ˜\widetilde{{\mathcal{ {\mathcal R} }}}) is (left, right) compatible, ℛ=ℛ∗{\mathcal{ {\mathcal R} }}={{\mathcal{ {\mathcal R} }}}^{\ast } or ℒ=ℒ˜{\mathcal{ {\mathcal L} }}=\widetilde{{\mathcal{ {\mathcal L} }}}. Then, we give the sufficient and necessary conditions such that TE∗(X){T}_{{E}^{\ast }}\left(X) is left regular (respectively, right regular, completely regular, intra-regular, and completely simple). Finally, we characterize the ℱ{\mathcal{ {\mathcal F} }}-abundant (respectively, G{\mathcal{G}}-abundant) and left (respectively, right) magnifying elements in TE∗(X){T}_{{E}^{\ast }}\left(X).

ABSTRACT: Plumage color is an important economic trait for breed feature identification and consumer's requirements in pigeons. The domestic pigeon has multiple types of plumage color, thereby providing a unique opportunity to identify the genetic basis of plumage coloration. White feather color is common for meat and medicinal use. To investigate the genetic variation associated with white plumage color in pigeons, we use genome resequencing and population genomics to identify the genomic regions with strong selective signature between pigeons with brown and white plumage color. Meanwhile, we obtained some candidate genes with melanin or melanosome biosynthesis in selected regions. Finally, we identified a missense mutation p.E256K in the EDNRB2 completely associated with white plumage color. These findings provide a basis for genetic variation in pigeons with plumage color phenotype.

Objective: This study aimed to develop a green and environment-friendly sodium alginate/starch/tea powder composite membrane. Methods: Tea polysaccharides with different mass fractions were added to the sodium alginate/starch/tea powder composite film to investigate the influence of tea polysaccharides on the properties of sodium alginate/starch/tea powder composite film. Therefore, full infrared barrier, color difference, water solubility, water vapor permeability, tensile strength, elongation at break, thickness, water content, free radical clearance and bacteriostasis were measured. Results: A strong interaction between tea polysaccharides and sodium alginate were found. The composite film with tea polysaccharide content of 5% exhibited the best comprehensive performance. The best bacteriostatic performance was observed when the tensile strength, elongation at break, water vapor permeability were 9.71 MPa, 25.42%, and 2.38×10-9 g·mm/(cm2·d·Pa), respectively. The antioxidant activity of the composite membrane increased with the increase of the amount of tea polysaccharide added, with a maximum of 85.49%. Conclusion: Tea polysaccharides can improve physicochemical properties, antibacterial and antioxidant properties of the composite membrane. When the mass fraction of tea polysaccharides was 5.00%, the composite membrane had the best mechanical, moisture, and antibacterial properties.

Objective: Aiming at the problems of unstable 3D printing quality and low printing efficiency of adipose tissue part of artificial meat based on plant raw materials, studied and improve the intelligent 3D printing trajectory motion algorithm matching with food materials, to reduce the accumulation and corners in the printing path. Methods: A trajectory planning method adapted to the characteristics of artificial meat adipose tissue raw materials was proposed. The complex section layer of the sliced model was divided into several simple polygon sub-partition contours using Bayesian algorithm, and an improved nearest neighbor method was used to determine the optimal motion starting point of each contour. At the same time, the genetic algorithm was integrated into the ant colony algorithm was applied to solve the optimal printing path of the partition contour, and finally the interior of the contour was filled with the improved zigzag scanning algorithm. Results: For the artificial meat adipose tissue in this study, through experimental verification, comparing the existing trajectory planning methods of Cura and Prusa, the established model was printed on a single-layer cross-section, and the printing time and motion path length were reduced, and the quality was good. Conclusion: The proposed trajectory planning algorithm for 3D printing of artificial meat adipose tissue has high feasibility and effectiveness.

The electrolytic separation of actinides(An) from fission products(FPs) on solid Al cathode is one of the most promising pyrochemical reprocessing processes. In this work, the electrochemical behaviour of Pu3+ on active Al solid cathode was studied through the transient electrochemical method. Based on the results of cyclic voltammetry(CV) and square wave voltammetry(SWV), Pu3+ can be reduced into alloy through a onestep threeelectrons transfer process on Al cathode, and the reaction is irreversible. The relationship between the reduction potential of the alloy formed by Pu3+ with Al and temperature is EΘ,*(Pu3+/PuAln)(vs. Cl-/Cl2)=-2.944+9.84×10-4T. Five Pu alloys(involving Pu3Al, PuAl, PuAl2, PuAl3, and PuAl4) are formed according to the results of open circuit chronopotentiometry(OCP) together with phase diagrams of Pu-Al. Furthermore, the formation Gibbs free energies of PuAl4 at different temperature are calculated.

To optimize the gas transfer, adsorption and photo-generated charge separation in the process of photocatalytic CO2 reduction by g-C3N4, the photocatalytic materials were designed from the aspects of foam pore structure and heterojunction construction. The typical g-C3N4 foam was first constructed using surfactant foaming method, and then Cu(OH)2 nanosheets were loaded to prepare the Cu(OH)2/CNF composites with projects of electroless copper plating and hydrogen oxidation treatment. The structure and photocatalytic properties of the as-prepared samples were investigated. The results show that g-C3N4 foam and Cu(OH)2/CNF all demonstrate developed structures with 3D micron pore frameworks, which is conducive to improving CO2 diffusion and adsorption at dynamics during gas-solid catalytic process. The adsorption amounts of CO2 for g-C3N4 foam and Cu(OH)2/CNF are respectively 3.97 cm3/g and 3.59 cm3/g, which are 2.96 times and 2.68 times respectively higher than that of pure g-C3N4 powder. Moreover, many Cu(OH)2 nanosheets are also formed in the Cu(OH)2/CNF samples which provide a way to simultaneously broaden light absorption and form heterojunction between g-C3N4 and Cu(OH)2. This heterojunction can accelerate the separation of photo-generated e--h+ and make photo-generated electrons transfer from g-C3N4 to Cu(OH)2. As a result, the Cu(OH)2/CNF has demonstrated optimal photocatalytic activity with CO production rate at 11.041 μmol·g-1·h-1, which is 2.76 times and 6.83 times respectively higher than that of g-C3N4 foam and g-C3N4 powder.

Background: Healthcare workers (HCWs) without evidence of immunity to varicella-zoster virus (VZV) are recommended to undergo varicella vaccination. Immunogenicity of live attenuated varicella vaccine has rarely been investigated among HCWs in Taiwan. Methods: Anti-VZV immunoglobulin G (IgG) titer was checked for all HCWs at Changhua Christian Hospital from 2011 to 2017. One-dose and two-dose (separated by 4–8 weeks) vaccines were administered to HCWs with equivocal and negative anti-varicella IgG results, respectively. Follow-up anti-VZV IgG was determined at least 4 weeks after completion of vaccination. Factors associated with seroconversion to varicella vaccination were analyzed. Results: Among 2406 included HCWs, the anti-VZV IgG serostatus was tested positive, equivocal and negative in 1924 (79.9%), 117 (4.9%) and 365 (15.2%), respectively. The seroprevalence had decreased from 88.0% (235/267) in 2011 to 72.2% (270/374) in 2017 (p for trend

Background and Aim: Staphylococcus aureus and Staphylococcus pseudintermedius are widespread skin and mucous membrane colonizers and may cause opportunistic infections in humans and animals. This study aimed to identify and characterize methicillin-resistant S. aureus (MRSA) and methicillin-resistant S. pseudintermedius (MRSP) isolates from domestic and stray dogs and cats and pet owners in Malaysia using molecular epidemiology and antimicrobial profiling. Materials and Methods: Three hundred and fifty oral and nasal swabs were taken from pet and stray dogs and cats and pet owners; all samples were subjected to culture and biochemical tests and polymerase chain reaction; the selected isolates were put through disk diffusion test and multilocus sequence typing. Results: One S. aureus isolate and three S. pseudintermedius isolates were identified as MRSA and MRSP, respectively, of which the MRSA isolate and one of the MRSP isolates showed multidrug resistance and the remaining two MRSP isolates were resistant to one or two antimicrobials. Multilocus sequence typing showed that the MRSA isolate belongs to clonal complex (CC) 789, while for the MRSP isolates, two were in CC45 and one was a singleton. Conclusion: This study is the first study in Malaysia to perform molecular characterization of MRSP isolated from pet dogs and cats and pet owners. The outcomes of this study reveal that even healthy pet dogs and cats and their owners can be carriers of drug-resistant staphylococci, highlighting the role of pets and pet owners as carriers of MRSA and MRSP in Malaysia.

With the help of bolted joints to assemble a complex structure, carbon fiber reinforced polymer composite (CFRP) is widely used in various fields. However, stress concentration around holes at the bolted joints leads to a decrease in bearing capacity. Composites often result in mechanical degradation subjected to a complex hydrothermal environment. Therefore, to study the tensile behavior of CFRP bolted joints subjected to hydrothermal aging, the tensile tests are conducted carefully. The influence of aging time and temperature on tensile strength is investigated based on the response history, strain contour, and failure morphology. The failure mechanism is revealed via digital image correlation technology. Finally, the experimental results demonstrate that the bearing capacity of the structure in hydrothermal aging decreases significantly. Compared with the unaged specimens, the peak force of the specimens aged for 6 weeks at 25°C and 65°C is reduced by 22.79% and 35.63%, respectively. Under both the unaged and aged, the same bearing failure is found in the tensile tests of CFRP single-bolt single-lap joints.

The present-day in-situ stresses affect the drilling design, well pattern deployment, well completion modification, hydraulic fracturing and water injection of tight-oil sandstones. The measurement data of these stresses are commonly unavailable because of their high costs and limited core samples, therefore employing conventional logs for these stress determination is imperative for tight-oil sandstones. Firstly, the suitable calculation models for the present-day in-situ stress calculation by conventional logs were selected according to the geological characteristics of the sixth member of the Yanchang Formation (Chang 6) in Heshui area of the southern Ordos Basin, China. Then, the dynamic rock mechanical parameters were determined by conventional logs, and corrected by the static rock mechanical parameters obtained from the triaxial rock mechanical tests. Moreover, the pore fluid pressure was determined by the empirical formula method. Finally, the maximum and minimum horizontal compressive stresses (σ H and σ h ), and the vertical stress (σ v ) of six wells were calculated according to the selected models of these stresses, respectively. The present-day in-situ stresses, determined by the proposed method in the paper, were verified by those obtained from acoustic emission tests and finite-element numerical simulations with the relative errors of less than 10%. The results show that the magnitudes of σ H , σ h and the horizontal differential stress (σ H−h ) in the study area mainly range from 32 to 43 MPa, 23 to 37 MPa and 5 to 8 MPa, respectively. The magnitude of the three-dimensional present-day in-situ stress increases with the increase of depth. The average gradients of σ H, σ v and σ h are 0.018, 0.014 and 0.015 MPa/m, respectively, that is σ H >σ v >σ h . In this stress state, the hydraulic fractures, with a trend of little expansion towards multiple directions, are commonly developed at a small angle intersecting with the direction of σ H in the study area.

ObjectiveTo establish a system for simultaneous detection of miR-888 and miR-891a by droplet digital PCR （ddPCR）, and to evaluate its application value in semen identification.MethodsThe hydrolysis probes with different fluorescence modified reporter groups were designed to realize the detection of miR-888 and miR-891a by duplex ddPCR. A total of 75 samples of 5 body fluids （including peripheral blood, menstrual blood, semen, saliva and vaginal secretion） were detected. The difference analysis was conducted by Mann-Whitney U test. The semen differentiation ability of miR-888 and miR-891a was evaluated by ROC curve analysis and the optimal cut-off value was obtained.ResultsThere was no significant difference between the dual-plex assay and the single assay in this system. The detection sensitivity was up to 0.1 ng total RNA, and the intra- and inter-batch coefficients of variation were less than 15%. The expression levels of miR-888 and miR-891a detected by duplex ddPCR in semen were both higher than those in other body fluids. ROC curve analysis showed that the AUC of miR-888 was 0.976, the optimal cut-off value was 2.250 copies/μL, and the discrimination accuracy was 97.33%; the AUC of miR-891a was 1.000, the optimal cut-off value was 1.100 copies/μL, and the discrimination accuracy was 100%.ConclusionIn this study, a method for detection of miR-888 and miR-891a by duplex ddPCR was successfully established. The system has good stability and repeatability and can be used for semen identification. Both miR-888 and miR-891a have high ability to identify semen, and the discrimination accuracy of miR-891a is higher.

In this paper, the conversion of methanol and butanol and the differences in product distribution over H-SAPO-34 were investigated. Some important reaction intermediates were captured during the reaction process by using gas chromatography-mass spectrometry (GC-MS) and 13C cross polarization magic angle spinning nuclear magnetic resonance (13C CP MAS NMR) spectroscopy. In the methanol conversion process, ethene, propene and butene are the main products, while in the butanol conversion process, butanol is mainly dehydrated to form butene, and propene and butene are the main products in the initial stage. Light olefins are generated from both methanol and butanol conversion over H-SAPO-34. Furthermore, the aromatic species were observed in both retained species analysis and 13C CP MAS NMR, indicating that similar organic species confined in the H-SAPO-34 during the conversion of alcohols. Starting from different kinds of alcohols, the acid-catalysis environment in the confined space of H-SAPO-34 can catalyze the methanol and butanol conversion to produce light olefins.

Recurrent pregnancy loss (RPL) has become an important reproductive health issue worldwide. RPL affects about 2%–3% of reproductive-aged women, and makes serious threats to women’s physical and mental health. However, the etiology of approximately 50% of RPL cases remains unknown (unexplained RPL), which poses a big challenge for clinical management of these patients. RPL has been widely regarded as a complex disease where its etiology has been attributed to numerous factors. Heretofore, various risk factors for RPL have been identified, such as maternal ages, genetic factors, anatomical structural abnormalities, endocrine dysfunction, prethrombotic state, immunological factors, and infection. More importantly, development and applications of next generation sequencing technology have significantly expanded opportunities to discover chromosomal aberrations and single gene variants responsible for RPL, which provides new insight into its pathogenic mechanisms. Furthermore, based upon patients’ diagnostic evaluation and etiologic diagnosis, specific therapeutic recommendations have been established. This review will highlight current understanding and recent advances on RPL, with a special focus on the immunological and genetic etiologies, clinical diagnosis and therapeutic management.

In view of the isolation of the software and hardware teaching environment, isolation of various online teaching platforms and lack of coordination of data, it is not conducive for further promotion of ‘Internet Plus Teaching’ and the improvement of online teaching. The Trinity teaching platform, namely ‘Learning in Zhejiang University City College’ is built against the background of digitalisation reform in colleges and universities. The teaching platform integrates the smart classroom, the Zhiyun Classroom (online classroom) and the curriculum platform. The platform breaks through the limitation of time and space, connects the teaching process and implements the integrated ‘teaching, learning, evaluation and management’ based on data collaboration. It is fully applied in Zhejiang University City College, and is stable, efficient and convenient, with a high access rate and better interaction between teachers and students. It effectively implements closed-loop management of the teaching process, changes the educational form of teacher education, student learning and school management. It also adapts to the cultivation of innovative and individualised talents under the new situation, and further deepens reforms of ‘Internet Plus Teaching’. It provides a solid support for the whole process management of student learning.

Nanofluids have been widely used as a class of promising working fluids with excellent heat transfer properties. However, the theoretical research on the thermal enhancement mechanism of nanofluids is still in the preliminary stage. Fractional constitutive models provide a new powerful tool to investigate the superior mechanical and thermal properties of nanofluids owing to their advantages in depicting the memory and genetic properties of the system. Fractional nanofluid models have become one of the hot research topics in recent years as better control of flow behavior and heat transfer can be achieved by considering fractional derivatives. The existing studies have indicated that the results obtained by the fractional-order nanofluid model are more consistent with the experimental results than traditional integer-order models. The purpose of this review is to identify the advantages and applications of fractional nanofluid models. First, various definitions of fractional derivatives and correlations of flux utilized in nanofluid modeling are presented. Then, the recent researches on nanofluids with fractional derivatives are sorted and analyzed. The impacts of fractional parameters on flow behaviors and heat transfer enhancement are also highlighted according to the Buongiorno model as well as the Tiwari and Das nanofluid model with fractional operators. Finally, applications of fractional nanofluids in many emerging fields such as solar energy, seawater desalination, cancer therapy, and microfluidic devices are addressed in detail.

The ammonium ions produced by the reactions of relatively excessive dimethylhydroxylamine(DMHAN) with Fe3+, Ce4+ and Pu4+ in dilute nitric acid solutions at room temperature were determined by ion chromatographic method. The results show that the reactions between DMHAN and Fe3+, Ce4+ and Pu4+ all produce a certain amount of ammonium ramifications under acidic conditions, which includes (CH3)2NH+2, NH+4 and CH3NH+3. The reaction mechanism is as follows. When DMHAN reacts with Fe3+, Ce4+ and Pu4+ respectively, it can be oxidized and reduced simultaneously in the acidic solution for the -1 valence of N atom in DMHAN. Then part of DMHAN is reduced to (CH3)2NH+2, and it is further converted into NH+4 and CH3NH+3 partly. With the increase of redox potential of Fe3+, Pu4+ and Ce4+, the conversion ratio of DMHAN into amine ions increases and the proportion of (CH3)2NH+2 in total amine ions decreases, while the proportion of NH+4 in total amine ions increases.

Objective: This study aimed to provide a rapid and efficient pretreatment method to detect the mineral elements in infant formula milk powder. Methods: Low-temperature rapid wet digestion (digesting temperature was 120 ℃ and digesting time was 90 min) combined with the inductively coupled plasma optical emission spectroscopy (ICP-OES) was used to determine the concentrations of K, Na, Ca, Mg, P, Fe, Zn, Cu, and Mn in infant formula milk powder. Results: The linear correlation coefficients of standard curves for these nine elements were all over 0.999 9. This method was verified by the reference materials in infant formula milk powder, and the detection values of each element were all within the range of their characteristic values. Besides, the spiked recovery rate of elements in a commercial infant milk powder was verified. Its recovery rate was 95.0%~102.0% and the relative standard deviation was within 5%. Conclusion: This method was fast and efficient, which is suitable for the digestion of mineral elements in infant formula milk powder.

Reducing exploration risks and realizing scientific prospecting always have been frontier fields and research hotspots in the world of mineral exploration, the theory and method of ore prospecting prediction for exploration area is the valid channel to deal with this problem. Using this method, a geological model of ore prospecting can be established by combining the internal (geochemical behavior of elements) and external (types of geological processes) control factors for mineralization. The main components of the prospecting prediction model include geological bodies related to mineralization, metallogenetic structure planes and mineralization characteristics. Together with the results of special geological mapping, geophysical and geochemical exploration on large scale, orebodies have been located by synthetic information and explored by drilling. Case studies of the Lala copper deposit in Sichuan, Muhu–Maerkantu manganese ore deposit in Xinjiang and Aonaodaba tin-polymetallic deposit in Inner Mongolia, illustrate the effective application of this method in ore prospecting prediction.

From the perspective of geography, human trafficking is the abnormal migration of a population. With the rise of the "relational turn" in human geography, relational geography provides a complex multidimensional and topological relationship logic system for studying human trafficking, which could help with exploring the process and correlation between the phenomenon and subjects of human trafficking. In this study, we focused on human trafficking aimed at women and young children and reviewed the relational geography theory to obtain the theoretical framework for this problem. Subsequently, we deconstructed the human trafficking and "post-trafficking" context from the paradigm of space, place, and subjectivity. Then, we constructed a relational geography logic system for the study and post-trafficking interventions. We found that (1) the connotations of "gender" and "body" in a patriarchal culture provide the "soil" and "market" for human trafficking aimed at women and young children. Because of its smallness on the spatial scale, the body is an important entrance to the study of human trafficking. Spatial physical disciplines and power separations exist in the relationship network formed by human trafficking. (2) Human trafficking generates multiple diachronic influences on victims. Children and women who have been abducted face the reconstruction of local relations and intersubjectivity during the post-trafficking stage. (3) Human trafficking governance and post-trafficking interventions should be conducted with a view toward the relational geography logic system and the paradigm of relational space, local relations, and intersubjectivity in order to open logical channels from offenders and victims. The introduction of relational geography may provide theoretical and practical contributions to the study of human trafficking. Regarding the former, it could promote the theoretical study of human trafficking crimes from the traditional paradigm of space to relationship space, as well as from the local to local relations and the subjective to intersubjectivity. In terms of application, findings of this study may draw attention to the importance of spatial physical discipline and power separation in human trafficking when strategizing regarding governance and interventions. Additionally, findings of the study may contribute to fostering more effective interventions during the post-trafficking stage because of our focus on the subjective reconstruction of women and children who have been kidnapped.

The Heteracanthocephalidae Petrochenko, 1956 is a rare family of acanthocephalans mainly parasitic in fishes. The pattern of mitogenomic evolution of the Heteracanthocephalidae is still unknown, and the phylogenetic relationships of the Heteracanthocephalidae with the other 14 families within the order Echinorhynchida remain unclear. In the present study, the complete mitochondrial genome of Aspersentis megarhynchus (von Linstow, 1892) Golvan, 1960 was sequenced and annotated for the first time, which represents the first mitogenomic data for the genus Aspersentis and also for the family Heteracanthocephalidae. The mitogenome of A. megarhynchus has 14,661 bp and includes 36 genes, containing 12 protein-coding genes (PCGs) (missing atp8), 22 tRNA genes, and 2 ribosomal RNAs (rrnS and rrnL), plus two non-coding regions. Comparative mitochondrial genomic analysis revealed that the presence of translocations of several tRNA genes (trnV, trnE, and trnT) and the gene arrangement in the mitogenome of A. megarhynchus represents a new type in Acanthocephala. Moreover, the mitogenomic phylogenetic results based on concatenated amino acid sequences of 12 protein-coding genes strongly supported the validity of the Heteracanthocephalidae and suggested close affinity between the Heteracanthocephalidae and Echinorhynchidae in the order Echinorhynchida.

Species of the genus Rhabdias Stiles & Hassall, 1905 are common parasitic nematodes occurring in the lungs of amphibians and reptiles worldwide. In the present study, Rhabdias macrocephalum n. sp. is described using integrated morphological methods (light and scanning electron microscopy) and molecular approaches (sequencing of the nuclear 28S and ITS regions, and mitochondrial cox1, cox2, and 12S genes) based on specimens collected from the green striped tree dragon Diploderma splendidum (Barbour & Dunn) (Reptilia: Agamidae) in China. The complete mitochondrial genome of R. macrocephalum n. sp. was sequenced and annotated: it is 14,819 bp in length, including 12 protein coding genes (missing atp8), 22 tRNA genes, 2 rRNA genes and three non-coding regions. The gene arrangement of R. macrocephalum n. sp. is different from all of the currently available mitogenomes of nematodes and represents a novel type of mitochondrial gene arrangement reported in Nematoda. Molecular phylogenetic results based on the ITS + 28S data support the monophyly of Entomelas, Pneumonema, Serpentirhabdias, and Rhabdias, and showed R. macrocephalum n. sp. forming a most basal lineage in Rhabdias.

Since the pilot water use statistics survey in Zhejiang Province in 2015, the list of water users in the province’s water use statistics survey has reached 7,899, with industrial and domestic direct reporting accounting for 94% of the total. By sorting out the problems and analyzing the causes in the implementation process of the water use statistics survey system, this paper proposes methods to improve the collection of water use data from public water supply enterprises, the backflow analysis of statistical direct reporting data, and the detailed rules for data review, as well as measures to solve problems. It also looks forward to the construction of a water index system that can predict economic changes and its application.

The dissolution process of oxides is a key step in the pyrochemical process of spent oxide fuel by electrolyzing in molten salt. The dissolved products will provide feeds for subsequent separation and recovery of U and Pu. The solubility and dissolution rate of oxides in molten salt systems are generally low. In order to meet process requirements, it is usually necessary to introduce chlorinating reagents. With different chlorinating reagents, the dissolution mechanism is quite different. Through extensive literature investigation, the related principles and characteristics of various chlorinating reagents in the chlorination process were analyzed and compared, which provided guidance for the research on chlorination and dissolution of U and Pu oxides in China.

In order to detect whether the GPS is abnormal in the flight state of small size quad-rotor, a GPS spoofing detection algorithm based on data fusion is proposed. Firstly, the velocity data from the monocular camera is calculated by using the self-velocity measurement algorithm based on the improved pyramid optical flow method. Secondly, the speed and altitude data of the IMU sensor unit of the small size quad-rotor were obtained, exactly the speed data were fused with the velocity data measured by using the optical flow method to obtain the optimal velocity estimation at that time. Finally, the small size quad-rotor GPS sensor data information is obtained, the aircraft speed and altitude data are calculated, and comparing with the data calculated in the previous step, to determine whether the GPS data at this time is abnormal. The experimental results on Anafi show that the present GPS spoofing detection algorithm can quickly detect data anomalies during flight. It can be seen that the present algorithm can provide strong technical support for GPS spoofing detection of quad-rotor.

Venous malformation is a common congenital anomaly of vascular development, and endovascular sclerotherapy is currently the first-line treatment for it. Accurate puncture of the lesion is the key to endovascular treatment. Any deviation is likely to result in local ulcers, necrosis and other complications. We described one case, in which a newly developed real-time MRI navigation technology was introduced and sclerotherapy was successfully performed for deep lesions that were difficult to reach by conventional methods. This article reviewed the treatment process of this case, in order to provide guidance and help for venous malformations and other soft tissue diseases in the future.

In this study, the chlorination mechanism and influencing factors of uranium oxides(mainly U3O8) were studied by using CCl4 and HCl gas as chlorination reagents respectively. The chlorination of U3O8 powder was studied with CCl4 as chlorination reagent. The mechanism and kinetic behavior of the chlorination reaction were studied by thermogravimetric analysis. The main chlorination product is UCl4. The chlorination of different types and morphologies of uranium oxide by CCl4 was also studied. The UO2 pellets are difficult to chlorinate due to their very dense structure. UO2 powder and UO3 powder are easily chlorinated by CCl4, and the chlorination products are UCl4 and UCl6 respectively. The chlorination of U3O8 powders in LiCl-KCl molten salt system was carried out using HCl gas as the chlorination reagent. The effects of reaction temperature, chlorination time, HCl gas flow rate, feeding amount of U3O8 powder, and uranium oxide types and morphologies were studied. The results show that increasing the reaction temperature, prolonging the reaction time, and increasing the HCl gas flow rate are beneficial to the improvement of the chlorination rate. The process parameters of HCl gas chlorinated U3O8 powder are recommended as follows: the chlorination reaction temperature is 500 ℃, and the flow rate of HCl gas is 0.6 L/min.

A Bayesian model updating and damage identification method based on Markov chain sampling was proposed for damage identification of stochastics structures. Firstly, an objective function based on the measured frequency and mode shapes was established, and then the Markov chain Monte Carlo method with delayed rejection was used for stochastics sampling, and the posterior probabilities of structural parameters in intact and damage states were obtained. By comparing the probability density functions of the identified parameters in the two states, the probability and degree of structural damage were finally obtained. The experimental example of steel cantilever beam indicated that based on the modified stochastics parameters, the damage probability and damage degree of each region of the structure are consistent with the real situation.

In this paper, the digital image based triaxial shear test, computed tomography (CT) scanning test and scanning electron microscopy (SEM) test were employed to investigate triaxial shear behavior and microstructure evolution of basalt fiber reinforced loess subjected to drying−wetting cycles. The results show that the shear failure mode of soil samples with relatively higher basalt fiber content changes from overall bulging failure to shear band failure with the increase in the number of drying−wetting cycles, while the shear failure mode exhibits an opposite trend with the increase in the fiber content at the early stage of drying−wetting process. Neither drying−wetting cycle nor fiber content has obvious effect on the type and characteristics of stress−strain curves, and both of them present strain-hardening behavior. The deviatoric stress at failure decreases with the increasing number of drying−wetting cycles; however, the attenuation rate gradually decreases. The deviatoric stress at failure first increases and then decreases with the increasing fiber content, showing a parabolic variation, and the optimal fiber content is 0.6%. A similar trend is observed between the ME value of CT scanning and the deviatoric stress at failure. Drying−wetting action induces cracking and loosening around the soil-fiber interface, thus weakening the fiber reinforcement effect. However, compared with unreinforced loess, fiber reinforced loess demonstrates strong overall stability in its microstructure. The macroscopic and microscopic damage variables reflecting the drying−wetting induced deterioration of loess samples were finally established, which shows a consistent variation trend.

A hydrodynamic model is developed for rainfall–runoff on asphalt pavement using two-dimensional shallow water equations. A simple yet precise expression is presented to compute flow velocity in order to alleviate the problems associated with numerical instabilities due to small water depths of thin sheet flow. The developed model performed well against measured data and numerical results in two segments. Then, the model was applied to study the influence of highway horizontal alignment, drainage manner, rainfall pattern, surface roughness and geometric parameters on pavement runoff. The results demonstrate that: (i) the influence of highway horizontal alignment on pavement runoff is nonsignificant, while that of drainage manner and the pavement surface roughness is significant. Great differences are observed in flow depth under concentrated drainage and overflow drainage conditions, especially in the area beyond 6 m away from the highway center axis; (ii) remarkable differences in maximum flow depth and peak runoff are presented under uneven and even rainfall conditions, while no great differences are found under three uneven rainfall conditions (front type, center front type and back front type); (iii) the sensitivity of the geometric parameters to the maximum flow depth from strong to weak is cross slope, width, slope length, and longitudinal slope under overflow drainage condition; while that is width, slope length, longitudinal slope and cross slope under concentrated drainage condition. HIGHLIGHTS A hydrodynamic model was developed for simulating rainfall–runoff on asphalt pavement.; A new solution of flow velocity was presented for thin sheet flow.; The influences of highway horizontal alignments, drainage manner, rainfall pattern, surface roughness and geometric parameters on pavement runoff were studied.;

Objective: This study aimed to eslablish a method for the determination of 15 benzodiazepine sedatives in aquatic products by liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. Methods: The sample was extracted with acetonitrile solvent, and the extract was purified by solid phase extraction （Oasis PRIME HLB） column. After concentration, the mobile phase was used to determine the volume. The mobile phase consisted of 1% formic acid aqueous solution and acetonitrile solvent. The method was performed in multiple reaction monitoring （MRM） mode and quantified by internal standard method. Results: The results showed that the linear range of 15 sedatives was 1~100 ng/mL （R＞0.99）. The limit of detection （LOD） was 0.1~0.3 μg/kg and the limit of quantification （LOQ） was 0.3~1.0 μg/kg. The average recoveries were 78%~125% and the relative standard deviations （RSD） were 1.52%~12.02%. Conclusion: The method has the advantages of simple pretreatment, good purification effect and accurate results. It is suitable for the rapid detection of 15 kinds of sedatives in aquatic products.

The 2021 WHO Classification of Tumors of the Central Nervous System (fifth edition) introduces a series of molecular biomarkers and new types/subtypes, which highlight the importance of integrated diagnoses and layered reports. The new edition tumors classification is reflections of the understanding in this field at present. Herein, we review the key molecular diagnostics and detection techniques, aiming to facilitate better understanding and more appropriate management for the tumors of the central nervous system.

Circular RNAs have been identified as crucial players in the initiation and progression of cancers, including colorectal cancer (CRC). The Has_circ_0000467 (circ_0000467) expression has been found to be upregulated in CRC, but its function and mechanism remain unclear.