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Abstract The mechanisms underlying tissue repair in response to damage have been one of main subjects of investigation. Here we leverage the wound-induced hair neogenesis (WIHN) models in adult mice to explore the correlation between degree of damage and the healing process and outcome. The multimodal analysis, in combination with single-cell RNA sequencing help to explore the difference in wounds of gentle and heavy damage degrees, identifying the potential role of toll-like receptor 9 (TLR9) in sensing the injury and regulating the immune reaction by promoting the migration of γδT cells. The TLR9 deficient mice or wounds injected with TLR9 antagonist have greatly impaired healing and lower WIHN levels. Inhibiting the migration of γδT cells or knockout of γδT cells also suppress the wound healing and regeneration, which can’t be rescued by TLR9agonist. Finally, the amphiregulin (AREG) is shown as one of most important effectors secreted by γδT cells and keratinocytes both in silicon or in the laboratory, whose expression influences WIHN levels and the expression of stem cell markers. In total, our findings reveal a previously unrecognized role for TLR9 in sensing skin injury and influencing the tissue repair and regeneration by modulation of the migration of γδT cells, and identify the TLR9-γδT cells-areg axis as new potential targets for enhancing tissue regeneration.

Plant anatomy is patterned early during leaf development which suggests studying the spatial–temporal transcriptomes of primordia will help identify critical regulative and functional genes. We successfully isolated the leaf primordia tissues from the C3 grass rice and the C4 grass foxtail millet by laser capture microdissection (LCM) and studied the gene expression throughout leaf developmental stages. Our data analysis uncovered the conserved expression patterns of certain gene clusters both in rice and foxtail millet during leaf development. We revealed genes and transcription factors involved in vein formation, stomatal development, and suberin accumulation. We identified 79 candidate genes associated with functional regulation of C4 anatomy formation. Screening phenotype of the candidate genes revealed that knock-out of a putative polar auxin transport related gene NAL1 resulted significantly reduced veinal space in rice leaf. Our present work provides a foundation for future analyses of genes with novel functions in grasses and their role in leaf development, in particular the role in leaves with a contrasting C3 vs. C4 biosynthetic pathway.

ObjectiveOne factor associated with rising rates of depression and anxiety among youth is a lack of meaning in life (MIL). The importance of living a meaningful and purposeful life cannot be overstressed, especially for adolescents who are in a critical life stage and have recently experienced a 3-year-long global health crisis, namely the COVID-19 pandemic. Although previous studies have examined adolescents’ MIL, the majority of them adopted a variable-centered approach. The present study adopts a person-centered approach to investigate the updated MIL among Chinese adolescents in the aftermath of the COVID-19 pandemic, the demographic factors related to profile classification, and the differences in mental health among adolescents in different MIL classes.MethodsA questionnaire assessing MIL, depression, anxiety, stress, and demographic information was administered to 1,196 Chinese adolescents (mean age = 13.07 ± 0.58; 44.73% were female individuals). A three-step latent profile analysis was conducted.Results and discussionThis study revealed that (1) adolescents can be categorized into three classes: high MIL, medium MIL, and low and searching MIL. (2) Adolescents from intact families with higher educated mothers were more likely to be classified as high MIL class rather than low MIL class. Furthermore, (3) adolescents with high MIL experienced the best mental health outcomes, those with medium MIL experienced moderate mental health, and those with low MIL, who were still searching for MIL, exhibited the poorest mental health. External support may be necessary in the search process for Chinese adolescents. Future research could explore the process of searching for MIL and identify the challenges adolescents encounter when developing their sense of MIL.

As distributed generation (DG) becomes increasingly integrated into the distribution grid, the structure of the distribution network is becoming more complex. To enhance the safety and cost-effectiveness of distribution systems, distribution network reconfiguration is gaining significant importance. Achieving optimal distribution network reconfiguration entails two key considerations: A feasible topology and economic efficiency. This paper addresses these challenges by introducing a novel approach that combines the potential island detection in undirected-graphs and the application of a whale optimization algorithm (WOA) for network reconfiguration optimization. To begin, we identified island categories based on the type of switchable-branches connected to these islands, allowing for the construction of potential island groups. Subsequently, unfeasible topologies were eliminated based on the conditions under which islands form within these potential island groups. Feasible topologies were then used to construct a model for network reconfiguration optimization. The optimal distribution network topology is determined using the WOA. In the final phase, the proposed method's effectiveness was demonstrated through a case study on the IEEE-33 node distribution network under scenarios with and without DG integration. The results showed that the proposed method exhibited better performance than traditional approaches in distribution network reconfiguration.

Abstract The preparation of pristine formamidinium lead tri‐iodide (FAPbI3) perovskite films for solar cells applications can be very challenging without methylammonium containing additives. The pinhole‐rich films prepared by methylammonium‐free approach limit the performance of the pristine FAPbI3 perovskite solar cells (PSCs). This work proposes a recrystallization method employing mixed solvent annealing (MSA) to heal the pinholes in pristine FAPbI3 perovskite films. The tetramethylene sulfoxide vapor is demonstrated most effective in grains‐merging and pinhole healing, while the addition of a secondary solvent vapor of anisole significantly improved the uniformity of TMSO vapor distribution. The pristine FAPbI3 PSCs prepared with MSA recrystallized film achieved power conversion efficiencies up to 22.43% and 16.54% on small cells (0.105 cm2) and mini‐modules (12.6 cm2), respectively, showing great potential in upscaling fabrication of perovskite photovoltaics with pristine FAPbI3.

IntroductionIntervertebral disc degeneration (IVDD) is a complex disease caused by genetic and environmental factors, but its pathogenesis is still unclear. Although studies of inflammatory cytokines have been used in recent years to unravel the biological mechanisms of a variety of diseases, such analyses have not yet been applied to IVDD. Therefore, we used a Mendelian Randomization approach to explore the potential mechanisms underlying the pathogenesis of IVDD.MethodsWe obtained GWAS data from publicly available databases for inflammatory cytokines and IVDD, respectively, and explored the causal relationship between individual inflammatory cytokines and IVDD using instrumental variable (IV) analysis. We primarily used IVW methods to assess causality, while sensitivity, heterogeneity and multidirectionality analyses were performed for positive results (p

Abstract The most abundant human milk oligosaccharide 2′‐fucosyllactose (2′‐FL) is a valuable component that has gained significant attention from the food industry. To biosynthesize 2′‐FL, various Escherichia coli K12 derivatives have been genetically modified. To further enhance the application performance of E. coli K12, a novel E. coli K12 derivative BL27 was used as a chassis cell in this study, and modular pathway enhancement was performed to achieve de novo synthesis of 2′‐FL. The futC gene encoding α‐1,2‐fucosyltransferase was introduced, and the wcaJ gene was knocked out to prevent the conversion of GDP‐ l‐fucose to colanic acid. Next, the effects of overexpressing transcriptional regulators rcsA and rcsB and knocking out transcriptional regulators mcbR and waaF were evaluated to optimize the colanic acid pathway. The expression level, solubility, and activity of FutC were improved through genomic integration, TrxA‐tag fusion, and double mutation in F40S/Q237S. Fermentation conditions were optimized to achieve maximum 2′‐FL titers of 3.86 and 23.56 g/L in shake‐flask and fed‐batch cultivation, respectively. Over 85% of the products were successfully excreted into extracellular and almost no byproduct 2′,3‐difucosyllactose was generated. This study has explored a new microbial platform and modification strategies for the synthesis of 2′‐FL and provides opportunities for its commercial production.

Abstract The present paper contains the investigations with respect to the optical appearance, redshift distribution, and observed energy flux of the thin accretion disk described by the Novikov-Thorne model in modified gravity (MOG) proposed by Moffat. The results show that the gravity coupling parameter $$\alpha $$ α plays an indispensable role in the size of direct and secondary images of the accretion disk, while the image profile is mainly affected by the observational angles. In addition, an increase of the parameter $$\alpha $$ α leads to that of the redshift factor, but a decrease in the radiation energy flux of the accretion disk. These results are due to the enhancement of the parameter $$\alpha $$ α that strengthens the black hole’s gravitational effects. By comparing the accretion disk images of the Schwarzschild-MOG black hole, the Schwarzschild black hole, and the Reissner–Nordström black hole, we point out that the Schwarzschild-MOG black hole exhibits the largest inner shadow and the lowest luminosity. This finding provides a potential way to distinguish Schwarzschild-MOG black holes from their counterparts in General Relativity.

Finite Control Set Model Predictive Control (FCS-MPC) has the ability to achieve multi-objective optimization, but there are still many challenges. The key to realizing multi-objective optimization in FCS-MPC lies in the design of the cost function. However, the different dimensions of penalty terms in the cost function often lead to difficulties in designing weighting coefficients. Incorrect weighting coefficients may result in truncation errors in calculations of DSPs and FPGAs, thereby affecting the algorithm’s control performance. Therefore, this article focuses on a system driving an induction motor with a three-level Neutral Point Clamped (NPC) inverter, and selects stator current and switching frequency as penalty terms in the cost function. An improved method is proposed to unify the dimensions of both penalty terms in the cost function. By unifying the dimensions of the penalty terms, a simple design of weighting coefficients can be achieved. Subsequently, to balance the inverter’s switching frequency and the dynamic response performance of the motor, a composite cost function is further proposed. Finally, the rationality of the proposed method is validated through simulation and experimental platforms.

Abstract Objective To evaluate the early clinical effect of oblique lumbar interbody fusion with lateral screw fixation and percutaneous endoscopic transforaminal discectomy (OLIF-PETD) in the treatment of lumbar disc herniation with lumbar instability. Methods A total of 22 patients with lumbar disc herniation and lumbar instability from August 2017 to August 2019 were enrolled in this retrospective study. The general information, perioperative indicators and complications were recorded. The clinical outcomes and radiological outcomes were evaluated before the operation, seven days after the operation, and at the last follow-up. Vertebral fusion and degree of multifidus muscle injury were evaluated at the last follow-up. Results In this study, OLIF + PETD showed shorter incision length compared to the MIS-TLIF (P 0.05), but OLIF + PETD could avoid the multifidus injury (P

Abstract We study the influence of parameter $$\alpha $$ α on the optical features of Schwarzschild-MOG black holes with different thin accretions in scalar-tensor-vector gravity. As $$\alpha $$ α increases from 0, the radii of the event horizon, photon sphere, and observed shadow increase in comparison with the Schwarzschild black hole. We constrain the parameter $$\alpha $$ α with the experimental data reported by the Event Horizon Telescope Collaboration for M87 $$^{*}$$ ∗ and Sagittarius A $$^{*}$$ ∗ . In the situation of spherical accretions, we unveil that the parameter $$\alpha $$ α has a positive effect on the shadow size but a negative effect on the observed specific intensities. Considering that the Schwarzschild-MOG black hole is surrounded by an optical and geometrically thin accretion disk, we find that the total observed specific intensities are mainly contributed by the direct emissions, while the photon rings and lensed rings provide small contributions. It is also found that with the increase of $$\alpha $$ α , the black hole shadow expands, the photon rings and lensed rings become larger and thicker. Besides, we emphasize that the boundary of the observed shadow cast by the aim black hole in the disk accretion scenario is determined by the direct emissions rather than the photon ring emissions. Consequently, we unveil that there is a linear relationship involving the critical impact parameter and the starting point of the direct emissions. This finding helps to use the experimental results of the Event Horizon Telescope to infer the critical impact parameter and to test General Relativity.

Augmented Reality Geographic Information System (ARGIS) applications can only provide users accurate content services with a highly precise geo-registration. However, the absolute 6DOF (Degree of Freedom) pose provided by the portable sensors is usually inaccurate in urban outdoors, resulting in poorly geo-registration accuracy for ARGIS applications. Aiming at this issue, an automatic vision-aided localization method based on the 2D map is proposed to improve the initial localization accuracy of the portable sensors, and an overall geo-registration optimization framework for outdoor ARGIS is proposed. Based on the initial pose provided by the sensors, the basic principles of the vision-aided localization method are expounded in detail. The experimental results show that the proposed method can effectively correct the initial pose obtained by the pose sensors, and improve the geo-registration accuracy of outdoor ARGIS applications ultimately.

ObjectivesThe Kaiser scoring system for breast magnetic resonance imaging is a clinical decision-making tool for diagnosing breast lesions. However, the Kaiser score (KS) did not include the evaluation of breast vascularity. Therefore, this study aimed to use KS combined with breast vascular assessment, defined as KS*, and investigate the effectiveness of KS* in differentiating benign from malignant breast lesions.MethodsThis retrospective study included 223 patients with suspicious breast lesions and pathologically verified results. The histopathological diagnostic criteria were according to the fifth edition of the WHO classification of breast tumors. The KS* was obtained after a joint evaluation combining the original KS and breast vasculature assessment. The receiver operating characteristic (ROC) curve was used for comparing differences in the diagnostic performance between KS* and KS, and the area under the receiver operating characteristic (AUC) was compared.ResultsThere were 119 (53.4%) benign and 104 (46.6%) malignant lesions in total. The overall sensitivity, specificity, and accuracy of increased ipsilateral breast vascularity were 69.2%, 76.5%, and 73.1%, respectively. The overall sensitivity, specificity, and accuracy of AVS were 82.7%, 76.5%, and 79.4%, respectively. For all lesions included the AUC of KS* was greater than that of KS (0.877 vs. 0.858, P = 0.016). The largest difference in AUC was observed in the non-mass subgroup (0.793 vs. 0.725, P = 0.029).ConclusionIpsilaterally increased breast vascularity and a positive AVS sign were significantly associated with malignancy. KS combined with breast vascular assessment can effectively improve the diagnostic ability of KS for breast lesions, especially for non-mass lesions.

The periosteum is known as the thin connective tissue covering most bone surfaces. Its extrusive bone regeneration capacity was confirmed from the very first century-old studies. Recently, pluripotent stem cells in the periosteum with unique physiological properties were unveiled. Existing in dynamic contexts and regulated by complex molecular networks, periosteal stem cells emerge as having strong capabilities of proliferation and multipotential differentiation. Through continuous exploration of studies, we are now starting to acquire more insight into the great potential of the periosteum in bone formation and repair in situ or ectopically. It is undeniable that the periosteum is developing further into a more promising strategy to be harnessed in bone tissue regeneration. Here, we summarized the development and structure of the periosteum, cell markers, and the biological features of periosteal stem cells. Then, we reviewed their pivotal role in bone repair and the underlying molecular regulation. The understanding of periosteum-related cellular and molecular content will help enhance future research efforts and application transformation of the periosteum.

Some fast radio bursts (FRBs) exhibit repetitive behaviors, and their origins remain enigmatic. It has been argued that repeating FRBs could be produced by the interaction between a neutron star and an asteroid belt. Here, we consider the systems in which an asteroid belt dwells around a massive star, while a neutron star, as a companion of the massive star, interacts with the belt through gravitational force. Various orbital configurations are assumed for the system. Direct N -body simulations are performed to investigate the dynamical evolution of the asteroids' belt. It is found that a larger orbital eccentricity of the neutron star will destroy the belt more quickly, with a large number of asteroids being scattered out of the system. A low inclination not only suppresses the collisions but also inhibits the ejection rate at early stages. However, highly inclined systems may undergo strong oscillations, resulting in the Kozai–Lidov instabilities. Among the various configurations, a clear periodicity is observed in the collision events for the case with an orbital eccentricity of 0.7 and mutual inclination of 0 ^◦ . It is found that such a periodicity can be sustained for at least eight neutron star orbital periods, supporting this mechanism as a possible explanation for periodically repeating FRBs. Our studies also suggest that the active stage of these kinds of FRB sources should be limited, since the asteroid belt would finally be destroyed by the neutron star after multiple passages.

NITROGEN LIMITATION ADAPTATION (NLA), a plasma-membrane-associated ubiquitin E3 ligase, plays a negative role in the control of the phosphate transporter family 1 (PHT1) members in Arabidopsis and rice. There are three NLA homologs in the Medicago truncatula genome, but it has been unclear whether the function of these homologs is conserved in legumes. Here we investigated the subcellular localization and the responses of MtNLAs to external phosphate and nitrate status. Similar to AtNLA1, MtNLA1/MtNLA2 was localized in the plasma membrane and nucleus. MtNLA3 has three alternative splicing variants, and intriguingly, MtNLA3.1, the dominant variant, was not able to target the plasma membrane, whereas MtNLA3.2 and MtNLA3.3 were capable of associating with the plasma membrane. In contrast with AtNLA1, we found that MtNLAs were not affected or even upregulated by low-phosphate treatment. We also found that MtNLA3 was upregulated by arbuscular mycorrhizal (AM) symbiosis, and overexpressing MtNLA3.1 in Medicago roots resulted in a decrease in the transcription levels of STR, an essential gene for arbuscule development. Taken together, our results highlight the difference between MtNLA homologs and AtNLA1. Further characterization will be required to reveal the regulation of these genes and their roles in the responses to external nutrient status and AM symbiosis.

Abstract Study design This study was a retrospective review. Objective To study the predictive effect of Hounsfield units (HU) value in the cervical vertebral body derived from computed tomography (CT) on the early titanium mesh cage (TMC) subsidence after anterior cervical corpectomy and fusion (ACCF). Methods This retrospective study was conducted on patients who underwent ACCF at one institution between January 2014 and December 2018. We collected date included age, gender, body mass index (BMI), disease type, surgical segment, whether merge ACDF, HU value of the vertebral body and endplate, vertebral body height loss, cervical lordosis angle, and cervical sagittal alignment. VAS, JOA, and NDI were used to assess clinical efficacy. Univariate analysis was performed to screen the influencing factors of TMC subsidence, and then logistic regression was used to find out the independent risk factors. The ROC curve and area under curve (AUC) were used to analyze the HU value to predict the TMC subsidence. Results A total of 85 patients who accepted ACCF were included in this study, and early titanium mesh cage subsidence was demonstrated in 29 patients. The subsidence rate was 34.1%. The JOA, VAS, and NDI scores significantly improved in both groups after the operation. Between the subsidence and non-subsidence groups, there were significant differences in age, intervertebral distraction height, and HU value in both upper and lower vertebral body and endplate. The logistic regression analysis proved that the HU value of the lower vertebral body was an independent risk of TMC subsidence, the AUC was 0.866, and the most appropriate threshold of the HU value was 275 (sensitivity: 87.5%, specificity: 79.3%). Conclusion Preoperative cervical CT value is an independent correlative factor for early TMC subsidence after ACCF, and patients with a low CT value of the inferior vertebral body of the operative segment have a higher risk of TMC subsidence in the early postoperative period. Trial registration: This study is undergoing retrospective registration.

IntroductionThe present study investigated how maternal and paternal controls, including behavioral and psychological controls, predict adolescent Internet addiction, as well as the potential moderating effects of adolescent gender and parent-child relationships on the predictions.MethodsData were collected from 1,974 Chinese adolescents (age range = 14–22; mean = 16.47; SD = 0.87; 1,099 girls) in Guizhou Province, mainland China in November 2021. Internet addiction was measured using the ten-item Internet Addiction Test developed by Kimberly Young, and Parental control and parent-child relationships were measured by the respective subscales derived from the validated Chinese Parent-Child Subsystem Quality Scale.ResultsHierarchical regression analyses revealed that after the covariates were statistically controlled, both parents' behavioral controls showed significant negative predictions on adolescent Internet addiction, while their psychological control tended to positively predict Internet addiction among adolescents, although the effect was only marginal. In addition, the impacts of maternal and paternal controls were equal, and such impacts did not vary between sons and daughters. While adolescent gender was not a significant moderator, the parent-child relationship quality significantly moderated the effects of paternal behavioral control, paternal psychological control, and maternal psychological control on adolescents' Internet addiction. Specifically, the prediction of paternal behavioral control was stronger while the effect of paternal and maternal psychological control was weaker among adolescents with a positive father-child relationship than those with a moderate or poor father-child relationship.DiscussionThese findings indicate the protective function of parents' behavioral control and the negative impact of psychological control on the development of adolescent Internet addiction. Further, a positive relationship between the father and the adolescent can strengthen the positive effect of paternal behavioral control and mitigate the negative effects of both parents' psychological controls.

ObjectiveComplete cervical spinal cord injury (CSCI) is a devastating injury that usually requires surgical treatment. Tracheostomy is an important supportive therapy for these patients. To evaluate the effectiveness of early one-stage tracheostomy during surgery compared with necessary tracheostomy after surgery, and to identify clinical factors for one-stage tracheostomy during surgery in complete cervical spinal cord injury.DesignData from 41 patients with complete CSCI treated with surgery were retrospectively analyzed.Participants and interventionsTen patients (24.4%) underwent one-stage tracheostomy during surgery, thirteen (31.7%) underwent tracheostomy when necessary after surgery, and eighteen (43.9%) did not have a tracheostomy.Main resultsOne-stage tracheostomy during surgery significantly reduced the development of pneumonia at 7 days after tracheostomy (p = 0.025), increased the PaO2 (p

Gait and weight carrying method may change the femoral neck load during stair descent. Applying specific gait and weight carrying methods may reduce the femoral neck load during stair descent, which may reduce hip pain, hip pain related falls and fall related fractures for the older population. The purpose of this study was to test the effect of different gait types (step-over-step v.s. step-by-step) and external weight carrying strategies (ipsilateral v.s. contralateral side) on the femoral neck load, discover which method could reduce the femoral neck load effectively. Seventeen healthy adults from 50 to 70 yrs old were recruited. The kinematic and kinetic analysis, musculoskeletal modelling method were used to estimate the joint and muscle loads for the lower extremities. Finite element analysis was used with the femur model to calculate the femoral neck strains during stair descent with different gait types and weight carrying strategies. The compressive strains were reduced for step-by-step gait method than step-over-step (p

Lower crust-derived granitic rocks provide constraints on the crustal reworking process and consequently give hints on the destruction mechanism of the cratons. The North China Craton (NCC) underwent extensive crustal melting in the Mesozoic. This study investigated granitic intrusions in the Dazeshan region of the Jiaodong Peninsula. Whole-rock major and trace element analyses and zircon U-Pb ages coupled with Hf isotopes were used to reveal the crustal reworking processes. Zircons separated from the quartz porphyry, Linglong granite, rhyolite porphyry, and biotite granite showed weighted mean 206Pb-238U ages of 119.2 ± 1.0 Ma, 140.2 ± 1.0 Ma, 120.6 ± 0.5 Ma, and 119.9 ± 0.7 Ma, respectively. The quartz porphyry, rhyolite porphyry, and biotite granite had high silica contents (SiO2 = 74–77 wt.%) but low MgO, Co, and Ni concentrations. The calculated εHf(t) values for the rhyolite porphyry and the biotite granite ranged from −18.3 to −20.0 and −17.8 to −20.2, respectively. These geochemical features imply ancient crust sources. The quartz porphyry showed distinct primitive mantle-normalized rare earth element (REE) patterns and was characterized by lower ΣREE content and lack of pronounced negative Eu anomalies. Whole-rock and zircon Dy/Yb ratios showed no correlation with whole-rock Rb/Sr ratios and zircon Hf contents, reflecting limited effects of crystal fractionation. The Ba/La ratios were also high (>150), but the Sr/Y and La/Yb ratios were low (Sr/Y < 50; La/Yb < 15). These features likely indicate that the quartz porphyry was generated by water-fluxed melting without differentiation. The rhyolite porphyry and biotite granite shared many geochemical similarities, denoting a unified source. The high La/Yb (>30) but low Sr/Y (

Arbuscular mycorrhizal fungi are obligate symbionts that transfer mineral nutrients to host plants through arbuscules, a fungal structure specialized for exchange for photosynthetic products. MtNF-YC6 and MtNF-YC11, which encode the C subunits of nuclear factor Y (NF-Y) family in Medicago truncatula are induced specifically by arbuscular mycorrhizal symbiosis (AMS). A previous study showed that MtNF-YC6 and MtNF-YC11 are activated in cortical cells of mycorrhizal roots, but the gene functions were unknown. Herein, we identified both MtNF-YB17 and MtNF-YB12 as the interacting partners of MtNF-YC6 and MtNF-YC11 in yeast and plants. MtNF-YB17 was highly induced by AMS and activated in cortical cells only in mycorrhizal roots but MtNF-YB12 was not affected. The formation of B/C heterodimers led the protein complexes to transfer from the cytoplasm to the nucleus. Silencing MtNF-YC6 and C11 by RNA interference (RNAi) resulted in decreased colonization efficiency and arbuscule richness. Coincidently, genes associated with arbuscule development and degeneration in RNAi roots were also downregulated. In silico analysis showed CCAAT-binding motifs in the promoter regions of downregulated genes, further supporting the involvement of NF-Y complexes in transcriptional regulation of symbiosis. Taken together, this study identifies MtNF-YC6- or MtNF-YC11-containing protein complexes as novel transcriptional regulators of symbiotic program and provides a list of potential downstream target genes. These data will help to further dissect the AMS regulatory network.

BackgroundTo date, the clinical need for therapeutic methods to prevent traumatic spinal cord injury (TSCI) progression and improve functional recovery has not been met. High mobility group box-1 (HMGB1) is released by necrotic neurons or secreted by glial cells after TSCI and plays an important role in pathophysiology.ObjectiveThe purpose of this study was to evaluate the effects of HMGB1-targeted therapy on locomotor function recovery, inflammation reduction, edema attenuation, and apoptosis reduction in rat and mouse models of TSCI.MethodsWe reviewed the literature on HMGB1-targeted therapy in the treatment and prognosis of TSCI. Twelve articles were identified and analyzed from four online databases (PubMed, Web of Science, Cochrane Library and Embase) based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and strict inclusion criteria.ResultsThe methodological quality of the 12 articles was poor. The results of the meta-analysis showed that compared with the SCI group, the treatment group had significantly increased locomotor function scores after SCI [n = 159, standardized mean difference (SMD) = 2.31, 95% confidence interval (CI) (1.52, 3.10), P < 0.00001], and the change in locomotor function scores was significantly increased in both the drug and anti-HMGB1 Ab groups (P < 0.000001 and P < 0.000001). A subgroup analysis showed significant differences (P > 0.05) between the drug group [(SMD) = 1.95, 95% CI (0.95, 2.94), P = 0.0001] and the anti-HMGB1 Ab group [(SMD) = 2.89, 95% CI (1.66, 4.13), P < 0.00001]. Compared with the SCI group, HMGB1 expression was significantly diminished [n = 76, SMD = −2.31, 95% CI (−3.71, −0.91), P = 0.001], TNF-α levels were significantly reduced [n = 76, SMD = −2.52, 95% CI (−3.77, −1.27), P < 0.0001], water content was significantly reduced [n = 44, SMD = −3.94, 95% CI (−6.28, −1.61), P = 0.0009], and the number of apoptotic cells was significantly diminished [n = 36, SMD = −3.31, 95% CI (−6.40, −0.22), P = 0.04] in the spinal cord of the treatment group.ConclusionHMGB1-targeted therapy improves locomotor function, reduces inflammation, attenuates edema, and reduces apoptosis in rats and mice with TSCI. Intrathecal injection of anti-HMGB1 Ab 0-3 h after SCI may be the most efficacious treatment.Systematic review registrationPROSPERO, identifier: CRD42022326114.

Accurate and robust localization using multi-modal sensors is crucial for autonomous driving applications. Although wheel encoder measurements can provide additional velocity information for visual-inertial odometry (VIO), the existing visual-inertial-wheel odometry (VIWO) still cannot avoid long-term drift caused by the low-precision attitude acquired by the gyroscope of a low-cost inertial measurement unit (IMU), especially in visually restricted scenes where the visual information cannot accurately correct for the IMU bias. In this work, leveraging the powerful data processing capability of deep learning, we propose a novel tightly coupled monocular visual-inertial-wheel odometry with neural gyroscope calibration (NGC) to obtain accurate, robust, and long-term localization for autonomous vehicles. First, to cure the drift of the gyroscope, we design a robust neural gyroscope calibration network for low-cost IMU gyroscope measurements (called NGC-Net). Following a carefully deduced mathematical calibration model, NGC-Net leverages the temporal convolutional network to extract different scale features from raw IMU measurements in the past and regress the gyroscope corrections to output the de-noised gyroscope. A series of experiments on public datasets show that our NGC-Net has better performance on gyroscope de-noising than learning methods and competes with state-of-the-art VIO methods. Moreover, based on the more accurate de-noised gyroscope, an effective strategy for combining the advantages of VIWO and NGC-Net outputs is proposed in a tightly coupled framework, which significantly improves the accuracy of the state-of-the-art VIO/VIWO methods. In long-term and large-scale urban environments, our RNGC-VIWO tracking system performs robustly, and experimental results demonstrate the superiority of our method in terms of robustness and accuracy.

Abstract For older population, a better understanding of the hip joint loading environment is needed for the prevention of hip pain, and the reduction of the stress fractures and fall risks. Using the motion analysis and inverse dynamics methods, combined with musculoskeletal modelling, static optimization, and finite element (FE) femur model, the difference of femoral neck strains between stair ascent vs. descent, young vs. older populations was compared. A two-way repeated-measures MANOVA was applied to test the effect of age and stair direction on the femoral neck strains. The strains at the femoral neck cross-section were greater for stair descent than ascent for both age groups (mostly P = 0.001 to 0006) but there was no difference between age groups. In this study, femoral neck strains represented bone loading environment in more direct ways than joint reaction forces/moments or joint contact forces, the risk of hip pain, falls and stress fractures might be greater during stair descent than ascent. Possible preventative methods to reduce these risks should be developed in the future studies.

With the exponential increase in the volume of automatic dependent surveillance-broadcast (ADS-B), and other types of air traffic control (ATC) data containing spatiotemporal attributes, it remains uncertain how to respond to immediate ATC data access within a target area. Accordingly, an original multi-level disaggregated framework for airspace, and its corresponding information management is proposed. Further, a multi-scale grid modeling and coding mapping method of airspace information represented by ADS-B is put forth. Finally, tests on the validity of the 4D airspace-temporal grid we named as the GeoSOT-AS framework were conducted across key areas based on the development of an effective data organization method for ADS-B, or an effective algorithm for extracting relevant spatiotemporal data. Experimentally, it was demonstrated that GeoSOT-AS conforms to the existing Chinese specification of civil aeronautical charting and is advantageous for its low deformation and high practicality; furthermore, the airspace grid identification code modeling was less costly, and improved performance by >80% when used for ADS-B data extraction. GeoSOT-AS can thus provide effective reference and practical information for existing airspace data management methods represented by ADS-B and can subsequently be extended to other forms of airspace management scenarios.

The X-ray afterglow of many gamma-ray bursts (GRBs) exhibits a plateau phase before the normal power-law decay stage, which may be related to continued activities of the central engine. Tang et al. collected 174 such GRBs and confirmed the so-called L – T – E correlation which involves three key parameters, i.e., the isotropic γ -ray energy E _γ _,iso of the prompt phase, the end time T _a of the plateau phase, and the corresponding X-ray luminosity L _X . In this study, the L – T – E correlation is confirmed and updated as ${L}_{{\rm{X}}}\propto {T}_{{\rm{a}}}^{-0.99}{E}_{\gamma ,\mathrm{iso}}^{0.86}$ with a large sample consisting of 210 plateau GRBs with known redshifts. The tight correlation is then applied to derive the pseudo-redshift of other 130 plateau GRBs whose redshifts are not directly measured. Statistical analysis is also carried out on this pseudo-redshift sample.

The metaverse provides us with an attractive virtual space in which the value of the virtual property has been increasingly recognized. However, the lack of effective cross-metaverse trading tools and the reputation guarantee makes it difficult to trade items among different metaverses. To this end, a decentralized reputation system for virtual items trading forum named DVIT is devised. To the best of our knowledge, DVIT is the first decentralized cross-metaverse item trading prototype inspired by the online-game trading system. We designed the corresponding transaction function and realized the autonomous governance of the community by introducing the reputation mechanism. An improved election mechanism is proposed to improve efficiency based on Delegated Proof-of-Stake (DPoS). Through token rewards associated with activity levels, users’ motivation can be stimulated. The experiments indicate that our proposed scheme could dynamically measure the trustworthiness degree of the users through the dynamic reputation value and thereby exclude malicious users from the blockchain within 20 epochs.

The discovery of numerous close-in planets has updated our knowledge of planet formation. The tidal interaction between planets and host stars has a significant impact on the orbital and rotational evolution of the close planets. Tidal evolution usually takes a long time and requires reliable numerical methods. The manifold correction method, which strictly satisfies the integrals dissipative quasiintegrals of the system, exhibits good numerical accuracy and stability in the quasi-Kepler problem. Different manifold correction methods adopt different integrals or integral invariant relations to correct the numerical solutions. We apply the uncorrected five- and six-order Runge–Kutta–Fehlberg algorithm [RKF5(6)], as well as corrected by the velocity scaling method and Fukushima’s linear transformation method to solve the tidal evolution of exoplanet systems. The results show that Fukushima’s linear transformation method exhibits the best performance in the accuracy of the semimajor axis and eccentricity. In addition, we predict the tidal timescale of several current close exoplanetary systems by using this method.

Leaf angle is one of the most important agronomic traits in rice, and changes in leaf angle can alter plant architecture to affect photosynthetic efficiency and thus determine grain yield. Therefore, it is important to identify key genes controlling leaf angle and elucidate the molecular mechanisms to improve rice yield. We obtained a mutant rela (regulator of leaf angle) with reduced leaf angle in rice by EMS mutagenesis, and map-based cloning revealed that OsRELA encodes a protein of unknown function. Coincidentally, DENSE AND ERECT PANICLE 2 (DEP2) was reported in a previous study with the same gene locus. RNA-seq analysis revealed that OsRELA is involved in regulating the expression of ILI and Expansin family genes. Biochemical and genetic analyses revealed that OsRELA is able to interact with OsLIC, a negative regulator of BR signaling, through its conserved C-terminal domain, which is essential for OsRELA function in rice. The binding of OsRELA can activate the expression of downstream genes repressed by OsLIC, such as OsILI1, a positive regulator of leaf inclination in rice. Therefore, our results suggest that OsRELA can act as a transcriptional regulator and is involved in the regulation of leaf inclination by regulating the transcriptional activity of OsLIC.

Summary: Biomethane is suggested as an advanced biofuel for the hard-to-abate sectors such as heavy transport. However, future systems that optimize the resource and production of biomethane have yet to be definitively defined. This paper assesses the opportunity of integrating anaerobic digestion (AD) with three emerging bioelectrochemical technologies in a circular cascading bioeconomy, including for power-to-gas AD (P2G-AD), microbial electrolysis cell AD (MEC-AD), and AD microbial electrosynthesis (AD-MES). The mass and energy flow of the three bioelectrochemical systems are compared with the conventional AD amine scrubber system depending on the availability of renewable electricity. An energy balance assessment indicates that P2G-AD, MEC-AD, and AD-MES circular cascading bioelectrochemical systems gain positive energy outputs by using electricity that would have been curtailed or constrained (equivalent to a primary energy factor of zero). This analysis of technological innovation, aids in the design of future cascading circular biosystems to produce sustainable advanced biofuels.

Glucosamine (GlcN) and its acetylated derivative N-acetylglucosamine (GlcNAc) are widely used in the pharmaceutical industries. Here, we attempted to achieve efficient production of GlcNAc via genomic engineering of Corynebacterium glutamicum. Specifically, we ligated the GNA1 gene, which converts GlcN-6-phosphate to GlcNAc-6-phosphate by transferring the acetyl group in Acetyl-CoA to the amino group of GlcN-6-phosphate, into the plasmid pJYW4 and then transformed this recombinant vector into the C. glutamicum ATCC 13032, ATCC 13869, ATCC 14067, and S9114 strains, and we assessed the GlcNAc titers at 0.5 g/L, 1.2 g/L, 0.8 g/L, and 3.1 g/L from each strain, respectively. This suggested that there were likely to be significant differences among the key genes in the glutamate and GlcNAc synthesis pathways of these C. glutamicum strains. Therefore, we performed whole genome sequencing of the S9114 strain, which has not been previously published, and found that there are many differences among the genes in the glutamate and GlcNAc synthesis pathways among the four strains tested. Next, nagA (encoding GlcNAc-6-phosphate deacetylase) and gamA (encoding GlcN-6-phosphate deaminase) were deleted in C. glutamicum S9114 to block the catabolism of intracellular GlcNAc, leading to a 54.8% increase in GlcNAc production (from 3.1 to 4.8 g/L) when grown in a shaker flask. In addition, lactate synthesis was blocked by knockout of ldh (encoding lactate dehydrogenase); thus, further increasing the GlcNAc titer to 5.4 g/L. Finally, we added a key gene of the GlcN synthetic pathway, glmS, from different sources into the expression vector pJYW-4-ceN, and the resulting recombinant strain CGGN2-GNA1-CgglmS produced the GlcNAc titer of 6.9 g/L. This is the first report concerning the metabolic engineering of C. glutamicum, and the results of this study provide a good starting point for further metabolic engineering to achieve industrial-scale production of GlcNAc. Keywords: C. glutamicum, Complete genome, N-acetylglucosamine, Metabolic engineering

Summary: Interspecies electron transfer is a fundamental factor determining the efficiency of anaerobic digestion (AD), which involves syntrophy between fermentative bacteria and methanogens. Direct interspecies electron transfer (DIET) induced by conductive materials can optimize this process offering a significant improvement over indirect electron transfer. Herein, conductive graphene was applied in the AD of protein-derived glycine to establish DIET. The electron-producing reaction via DIET is thermodynamically more favorable and exhibits a more negative Gibbs free energy value (−60.0 kJ/mol) than indirect hydrogen transfer (−33.4 kJ/mol). The Gompertz model indicated that the kinetic parameters exhibited linear correlations with graphene addition from 0.25 to 1.0 g/L, leading to the highest increase in peak biomethane production rate of 28%. Sedimentibacter (7.8% in abundance) and archaea Methanobacterium (71.1%) and Methanosarcina (11.3%) might be responsible for DIET. This research can open up DIET to a range of protein-rich substrates, such as algae. : Chemical Engineering; Environmental Chemical Engineering; Microbial Biotechnology; Nanomaterials Subject Areas: Chemical Engineering, Environmental Chemical Engineering, Microbial Biotechnology, Nanomaterials

Understanding the hip loading environment for daily activities is useful for hip fracture prevention, rehabilitation, and the design of osteogenic exercises. Seventeen older adults (50-70 yrs) and twenty young adults (18-30 yrs) were recruited. A rigid body model combined with a musculoskeletal model was used to estimate lower extremity loading. An elliptical cross-section model of the femoral neck was used to estimate femoral neck stress during stair ascent and descent. Two peaks were identified in the stress curves, corresponding to the peaks in the vertical ground reaction force. During stair ascent, significantly higher tension on the superior femoral neck was found for the young group at peak 1 (young: 13.5±6.1 MPa, older: 4.2±6.5 MPa, p

As a traditional amino acid producing bacterium, Corynebacterium glutamicum is a platform strain for production of various fine chemicals. Based on the CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system, gene editing tools that enable base conversion in the genome of C. glutamicum have been developed. However, some problems such as genomic instability caused by DNA double-strand break (DSB) and off-target effects need to be solved. In this study, a DSB-free single nucleotide genome editing system was developed by construction of a bi-directional base conversion tool TadA-dCas9-AID. This system includes cytosine base editors (CBEs): activation-induced cytidine deaminase (AID) and adenine deaminase (ABEs): tRNA adenosine deaminase (TadA), which can specifically target the gene through a 20-nt single guide RNA (sgRNA) and achieve the base conversion of C-T, C-G and A-G in the 28-bp editing window upstream of protospacer adjacent motif. Finally, as a proof-of-concept demonstration, the system was used to construct a mutant library of zwf gene in C. glutamicum S9114 genome to improve the production of a typical nutraceutical N-acetylglucosamine (GlcNAc). The GlcNAc titer of the mutant strain K293R was increased by 31.9% to 9.1 ​g/L in shake flask. Here, the developed bases conversion tool TadA-dCas9-AID does not need DNA double-strand break and homologous template, and is effective for genome editing and metabolic engineering in C. glutamicum.

Phospholipase Dα (PLDα), which produces signaling molecules phosphatidic acid (PA), has been shown to play a critical role in plants adapting to salt environments. However, it is unclear whether phospholipase Dδ (PLDδ) can mediate the salt response in higher plants. PePLDδ was isolated from salt-resistant Populus euphratica and transferred to Arabidopsis thaliana to testify the salt tolerance of transgenic plants. The NaCl treatment (130 mM) reduced the root growth and whole-plant fresh weight of wild-type (WT) A. thaliana, vector controls (VC) and PePLDδ-overexpressed lines, although a less pronounced effect was observed in transgenic plants. Under salt treatment, PePLDδ-transgenic Arabidopsis exhibited lower electrolyte leakage, malondialdehyde content and H2O2 levels than WT and VC, resulting from the activated antioxidant enzymes and upregulated transcripts of genes encoding superoxide dismutase, ascorbic acid peroxidase and peroxidase. In addition, PePLDδ-overexpressed plants increased the transcription of genes encoding the plasma membrane Na+/H+ antiporter (AtSOS1) and H+-ATPase (AtAHA2), which enabled transgenic plants to proceed with Na+ extrusion and reduce K+ loss under salinity. The capacity to regulate reactive oxygen species (ROS) and K+/Na+ homeostasis was associated with the abundance of specific PA species in plants overexpressing PePLDδ. PePLDδ-transgenic plants retained a typically higher abundance of PA species, 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2), 36:5 (18:2–18:3) and 36:6 (18:3–18:3), under control and saline conditions. It is noteworthy that PA species 34:2 (16:0–18:2), 34:3 (16:0–18:3), 36:4 (18:2–18:2) and 36:5 (18:2–18:3) markedly increased in response to NaCl in transgenic plants. In conclusion, we suppose that PePLDδ-derived PA enhanced the salinity tolerance by regulating ROS and K+/Na+ homeostasis in Arabidopsis.

Grass represents a major renewable feedstock in temperate climate zones, but its efficient utilization is challenging in biorefineries and advanced biofuels due to its structural recalcitrance. Here hydrothermal hydrolysis (100–180°C, for up to 40 min duration) was investigated to improve sugar yields from grass silage. The optimal conditions (140°C for 20 min duration) showed the highest sugar yield of 0.29 g/g volatile solid (VS) of grass silage. Further increasing the temperature to 180°C favored degradation of sugars (such as glucose, xylose) to by-products (such as furfural, hydroxymethylfurfural). A first-order reaction model confirmed a two-step reaction with the first step hydrolysis and the second step degradation. An energy balance calculation indicated that pre-treatment at 140°C required an energy input of 16.5 kJ/g VS, which could be significantly reduced to 5.1 kJ/g VS through efficient heat recovery. This research assists in understanding of the hydrolysis mechanism and provides a practical solution to produce grass-based sugars for further advanced biofuel and biorefinery applications.

Cadmium (Cd2+) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd2+-hyperaccumulator, Populus × canescens, is relatively salt-sensitive and therefore cannot be directly used to remediate HMs from salt-affected soils. The aim of the present study was to testify whether colonization of P. × canescens with ectomycorrhizal (EM) fungi, a strategy known to enhance salt tolerance, provides an opportunity for affordable remediation of Cd2+-polluted saline soils. Ectomycorrhization with Paxillus involutus strains facilitated Cd2+ enrichment in P. × canescens upon CdCl2 exposures (50 μM, 30 min to 24 h). The fungus-stimulated Cd2+ in roots was significantly restricted by inhibitors of plasmalemma H+-ATPases and Ca2+-permeable channels (CaPCs), but stimulated by an activator of plasmalemma H+-ATPases. NaCl (100 mM) lowered the transient and steady-state Cd2+ influx in roots and fungal mycelia. Noteworthy, P. involutus colonization partly reverted the salt suppression of Cd2+ uptake in poplar roots. EM fungus colonization upregulated transcription of plasmalemma H+-ATPases (PcHA4, 8, 11) and annexins (PcANN1, 2, 4), which might mediate Cd2+ conductance through CaPCs. EM roots retained relatively highly expressed PcHAs and PcANNs, thus facilitating Cd2+ enrichment under co-occurring stress of cadmium and salinity. We conclude that ectomycorrhization of woody hyperaccumulator species such as poplar could improve phytoremediation of Cd2+ in salt-affected areas.

We mainly focus on the effects of small changes of parameters on the dynamics of charged particles around Kerr black holes surrounded by an external magnetic field, which can be considered as a tidal environment. The radial motions of charged particles on the equatorial plane are studied via an effective potential. It is found that the particle energies at the local maxima values of the effective potentials increase with an increase in the black hole spin and the particle angular momenta, but decrease with an increase of one of the inductive charge parameter and magnetic field parameter. The radii of stable circular orbits on the equatorial plane also increase, whereas those of the innermost stable circular orbits decrease. On the other hand, the effects of small variations of the parameters on the orbital regular and chaotic dynamics of charged particles on the non-equatorial plane are traced by means of a time-transformed explicit symplectic integrator, Poincaré sections and fast Lyapunov indicators. It is shown that the dynamics sensitivity depends on small variations in the inductive charge parameter, magnetic field parameter, energy, and angular momentum. Chaos occurs easily as each of the inductive charge parameter, magnetic field parameter, and energy increases but is weakened as the angular momentum increases. When the dragging effects of the spacetime increase, the chaotic properties are not always weakened under some circumstances.

This work aimed at investigating the interactive effects of salt-signaling molecules, i.e., ethylene, extracellular ATP (eATP), H2O2, and cytosolic Ca2+ ([Ca2+]cyt), on the regulation of K+/Na+ homeostasis in Arabidopsisthaliana. The presence of eATP shortened Col-0 hypocotyl length under no-salt conditions. Moreover, eATP decreased relative electrolyte leakage and lengthened root length significantly in salt-treated Col-0 plants but had no obvious effects on the ethylene-insensitive mutants etr1-1 and ein3-1eil1-1. Steady-state ionic flux kinetics showed that exogenous 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) and eATP-Na2 (an eATP donor) significantly increased Na+ extrusion and suppressed K+ loss during short-term NaCl treatment. Moreover, ACC remarkably raised the fluorescence intensity of salt-elicited H2O2 and cytosolic Ca2+. Our qPCR data revealed that during 12 h of NaCl stress, application of ACC increased the expression of AtSOS1 and AtAHA1, which encode the plasma membrane (PM) Na+/H+ antiporters (SOS1) and H+-ATPase (H+ pumps), respectively. In addition, eATP markedly increased the transcription of AtEIN3, AtEIL1, and AtETR1, and ACC treatment of Col-0 roots under NaCl stress conditions caused upregulation of AtRbohF and AtSOS2/3, which directly contribute to the H2O2 and Ca2+ signaling pathways, respectively. Briefly, ethylene was triggered by eATP, a novel upstream signaling component, which then activated and strengthened the H2O2 and Ca2+ signaling pathways to maintain K+/Na+ homeostasis under salinity.

Non-invasive micro-test techniques (NMT) were used to analyze NaCl-altered flux profiles of K+, Na+, and H+ in roots and effects of NaHS (a H2S donor) on root ion fluxes in two contrasting poplar species, Populus euphratica (salt-resistant) and Populus popularis (salt-sensitive). Both poplar species displayed a net K+ efflux after exposure to salt shock (100 mM NaCl), as well as after short-term (24 h), and long-term (LT) (5 days) saline treatment (50 mM NaCl, referred to as salt stress). NaHS (50 μM) restricted NaCl-induced K+ efflux in roots irrespective of the duration of salt exposure, but K+ efflux was not pronounced in data collected from the LT salt stress treatment of P. euphratica. The NaCl-induced K+ efflux was inhibited by a K+ channel blocker, tetraethylammonium chloride (TEA) in P. popularis root samples, but K+ loss increased with a specific inhibitor of plasma membrane (PM) H+-ATPase, sodium orthovanadate, in both poplar species under LT salt stress and NaHS treatment. This indicates that NaCl-induced K+ loss was through depolarization-activated K+ channels. NaHS caused increased Na+ efflux and a corresponding increase in H+ influx for poplar roots subjected to both the short- and LT salt stress. The NaHS-enhanced H+ influx was not significant in P. euphratica samples subjected to short term salt stress. Both sodium orthovanadate and amiloride (a Na+/H+ antiporter inhibitor) effectively inhibited the NaHS-augmented Na+ efflux, indicating that the H2S-enhanced Na+ efflux was due to active Na+ exclusion across the PM. We therefore conclude that the beneficial effects of H2S probably arise from upward regulation of the Na+/H+ antiport system (H+ pumps and Na+/H+ antiporters), which promote exchange of Na+ with H+ across the PM and simultaneously restricted the channel-mediated K+ loss that activated by membrane depolarization.

To understand the knowledge of liner thermal expansion and thermal expansion coefficient of Q450NQR1 casting steel, dilatometry experiments were performed at two different cooling rates of 5℃·min-1 and 20℃·min-1. A mathematical model was developed to quantitatively extract the kinetic information of austenite transformation based on the concept of average atomic volume. The model was verified by microstructure examinations, indicating that the model was appropriate to describe the behavior of austenite transformation and could be applied to multi-phase transformations during steel continuous casting. The influence of cooling rate on the austenite transformation was discussed with the model. it is found that, with increasing cooling rate, two peaks associated to the precipitation of ferrite and pearlite on the thermal expansion coefficient curve shifted towards a lower temperature region with higher values. As the cooling rate increases from 5℃·min-1 to 20℃·min-1, the initial temperatures of ferrite and pearlite precipitation decrease by 32℃ and 37℃, and the final volume fractions of ferrite and pearlite gradually change from 0.894 and 0.106 to 0.945 and 0.055, respectively.

We investigated the effects of salt-sensitive signaling molecules on ionic fluxes and gene expression related to K+/Na+ homeostasis in a perennial herb, Glycyrrhiza uralensis, during short-term NaCl stress (100 mM, 24 h). Salt treatment caused more pronounced Na+ accumulation in root cells than in leaf cells. Na+ ions were mostly compartmentalized in vacuoles. Roots exposed to NaCl showed increased levels of extracellular ATP (eATP), cytosolic Ca2+, H2O2, and NO. Steady-state flux recordings revealed that these salt-sensitive signaling molecules enhanced NaCl-responsive Na+ efflux, due to the activated Na+/H+ antiport system in the plasma membrane (PM). Moreover, salt-elicited K+ efflux, which was mediated by depolarization-activated cation channels, was reduced with the addition of Ca2+, H2O2, NO, and eATP. The salt-adaptive effects of these molecules (Na+ extrusion and K+ maintenance) were reduced by pharmacological agents, including LaCl3 (a PM Ca2+ channel inhibitor), DMTU (a reactive oxygen species scavenger), cPTIO (an NO scavenger), or PPADS (an antagonist of animal PM purine P2 receptors). RT-qPCR data showed that the activation of the PM Na+/H+ antiport system in salinized roots most likely resulted from the upregulation of two genes, GuSOS1 and GuAHA, which encoded the PM Na+/H+ antiporter, salt overly sensitive 1 (SOS1), and H+-ATPase, respectively. Clear interactions occurred between these salt-sensitive agonists to accelerate transcription of salt-responsive signaling pathway genes in G. uralensis roots. For example, Ca2+, H2O2, NO, and eATP promoted transcription of GuSOS3 (salt overly sensitive 3) and/or GuCIPK (CBL-interacting protein kinase) to activate the predominant Ca2+-SOS signaling pathway in salinized liquorice roots. eATP, a novel player in the salt response of G. uralensis, increased the transcription of GuSOS3, GuCIPK, GuRbohD (respiratory burst oxidase homolog protein D), GuNIR (nitrate reductase), GuMAPK3, and GuMAPK6 (the mitogen-activated protein kinases 3 and 6). Moreover, GuMAPK3 and GuMAPK6 expression levels were enhanced by H2O2 in NaCl-stressed G. uralensis roots. Our results indicated that eATP triggered downstream components and interacted with Ca2+, H2O2, and NO signaling to maintain K+/Na+ homeostasis. We propose that a multiple signaling network regulated K+/Na+ homeostasis in NaCl-stressed G. uralensis roots.