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213 results on '"Chen, Zhaoguo"'

9. A low testosterone level impairs erectile function by increasing endocan expression in rat penile corpus cavernosum.

Author

Chen, Zhaoguo, Jiang, Jun, and Jiang, Rui

Subjects
*NITRIC-oxide synthases, *CASTRATION, *ENDOTHELIAL cells, *IMPOTENCE, *TESTOSTERONE
Abstract

Background: The mechanism by which a state of low testosterone leads to erectile dysfunction (ED) has not been determined. Endocan is a novel marker of endothelial function. However, whether endocan is involved in the regulation of erectile function under low testosterone levels remains unclear. Aim: In this study we sought to determine whether a low-testosterone state inhibits erectile function by regulating endocan expression in the endothelial cells of the rat penile corpus cavernosum. Methods: Thirty-six male Sprague–Dawley rats aged 8 weeks were randomly assigned to 6 groups (n = 6 per group) as follows: (1) control, (2) castration, (3) castration + testosterone treatment (treated with 3 mg/kg testosterone propionate per 2 days), (4) control + transfection (4 weeks after castration, injected with lentiviral vector (1 × 108 transduction units/mL, 10 μL), (5) castration + transfection, or (6) castration + empty transfection. One week after the injection, we measured the maximal intracavernous pressure/mean arterial pressure (ICPmax/MAP), serum testosterone and nitric oxide (NO) levels, and the expression of endocan, phospho–endothelial NO synthase (p-eNOS), eNOS, phospho–protein kinase B (p-AKT), and AKT in the rat penile corpus cavernosum. Outcomes: Under a low-androgen state, the expression of endocan in the rat penile corpus cavernosum was significantly increased, which inhibited the AKT/eNOS/NO signaling pathway and resulted in ED. Results: In the castration group, the expression of endocan in the rat penile corpus cavernosum was significantly higher than that in the control group (P < .05). Additionally, the levels of p-AKT/AKT, p-eNOS/eNOS, and NO in the rat penile corpus cavernosum and ICPmax/MAP were significantly lower in the castration group than in the control group (P < .05). In the castration + transfection group compared with the castration group there was a significant decrease in the expression of endocan (P < .05) and an increase in the ratios of p-AKT/AKT, p-eNOS/eNOS, and ICPmax/MAP (P < .05) in the rat penile corpus cavernosum. Clinical implications: Downregulating the expression of endocan in the penile corpus cavernosum may be a feasible approach for treating ED caused by hypoandrogenism. Strengths and limitations: The results of this study indicte that endocan may affect NO levels and erectile function through multiple signaling pathways, but further experiments are needed to clarify the relationship between endocan and androgens. Conclusion: A low-testosterone state inhibits the AKT/eNOS/NO signaling pathway by increasing the expression of endocan in the rat penile corpus cavernosum and impairing erectile function in rats. Decreasing the expression of endocan in the penile corpus cavernosum can improve erectile function in rats with low testosterone levels. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Molecular characterization of Klebsiella pneumoniae in clinical bovine mastitis in 14 provinces in China

Author

Wusiman, Maierhaba, primary, Zuo, Jiakun, additional, Yu, Yong, additional, Lv, Zhaoyang, additional, Wang, Mengdi, additional, Nie, Lianhua, additional, Zhang, Xiuping, additional, Wu, Jing, additional, Jiang, Wei, additional, Pan, Zihao, additional, Zhang, Wei, additional, Yin, Huifang, additional, Huang, Cuiqin, additional, Chen, Zhaoguo, additional, Miao, Jinfeng, additional, Chen, Wei, additional, and Han, Xiangan, additional

Published
2023
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18. EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep

Author

Zhang, Xiaoli, primary, Wei, Chenxi, additional, Lv, Yajie, additional, Mi, Rongsheng, additional, Guo, Baoping, additional, Rahman, Sajid Ur, additional, Zhang, Yehua, additional, Cheng, Long, additional, Jia, Haiyan, additional, Huang, Yan, additional, Han, Xiangan, additional, Gong, Haiyan, additional, and Chen, Zhaoguo, additional

Published
2023
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23. Seroprevalence and associated risk factors of Toxoplasma gondii infection in yaks (Bos grunniens) on the Qinghai–Tibetan Plateau of China

Author

Sun Tao, Rahman Sajid Ur, Cai Jinzhong, Zeng Jiangyong, Mi Rongsheng, Zhang Yehua, Gong Haiyan, Ma Hongcai, Huang Yan, Han Xiangan, Zhao Quan, and Chen Zhaoguo

Subjects
toxoplasma gondii, yak, seroprevalence, risk factors, qinghai–tibetan plateau, Infectious and parasitic diseases, RC109-216
Abstract

Toxoplasma gondii is an intracellular parasite that is extensively prevalent globally. Studies have indicated the presence of T. gondii infection in animals in some provinces of China, but little is known about T. gondii infection in yaks (Bos grunniens) on the Qinghai–Tibetan Plateau. In the current study, to determine the seroprevalence and associated risk factors of T. gondii, a total of 2784 serum samples were collected from 18 different sampling sites in eight counties of the Qinghai and Tibet regions of China from 2018 to 2019. Serum antibodies against T. gondii were detected in 261 yaks (9.38%) via enzyme-linked immunosorbent assay (ELISA). We found that seroprevalence differed significantly among different counties (ranging from 5.41% in Gangcha to 19.79% in Datong), by year in the Tibet Autonomous Region (from 2.34% in 2018 to 13.24% in 2019), and by age (from 5.59% in 0 < year ≤ 1 to 11.76% in year > 7) (p < 0.05). Climate, geographical conditions, and age are the main factors influencing T. gondii infection in yaks in these regions. Therefore, our study provides a data reference for public health and prevention of yak toxoplasmosis.

Published
2021
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24. Stress evaluation of ferromagnetic materials based on a new Barkhausen noise sensor composed by high entropy alloy magnetic core

Author

Qian, Zhengchun, Dong, Yawen, Yang, Chaolin, Zhang, Wei, Xu, Ruifeng, Chen, Zhaoguo, Xu, Hongmeng, Cheng, Huanbo, and Huang, Haihong

Abstract

During the service of ferromagnetic structural steel materials, stress should be evaluated accurately. Although the magnetic Barkhausen noise (MBN) testing has the ability to sense the stress, it can be easily interfered by environment. In this paper, a new MBN sensor is fabricated by selecting FeCoNi(AlMn)0.25 high entropy alloy (HEA) as the case of magnetic core to improve the accuracy of stress evaluation. The process optimization results show that the stability of MBN signal characteristics is the largest when the excitation frequency is 4Hz and the voltage is 6V. The signal-to-noise ratio (SNR) of MBN indicates that the HEA and Ni-Zn ferrite probes have better anti-inference capability. The MBN signal characteristic values peak voltage (PV) and root-mean-square (RMS) measured by HEA probe can linearly quantify the stress level with higher efficiency, stability and accuracy. The underlying reason of high sensitivity of HEA probe to the variation of MBN signals is revealed based on the magnetic properties. The microstructure and the thermodynamic parameters are analyzed to clarify the additions of Al and Mn atoms can affect the short-ranged magnetic exchange interaction and lattice distortion, which affects the magnetization behavior of HEA. Finally, the availability of MBN sensor with HEA magnetic core to the stress evaluation on the retired slide rails of car seats is conducted, which demonstrates its great application value.

Published
2024
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31. Effect of nutritional and environmental conditions on biofilm formation of avian pathogenic Escherichia coli

Author

Hu, Jiangang, primary, Lv, Xiaolong, additional, Niu, Xiangpeng, additional, Yu, Fangheng, additional, Zuo, Jiakun, additional, Bao, Yinli, additional, Yin, Huifang, additional, Huang, Cuiqin, additional, Nawaz, Saqib, additional, Zhou, Wen, additional, Jiang, Wei, additional, Chen, Zhaoguo, additional, Tu, Jian, additional, Qi, Kezong, additional, and Han, Xiangan, additional

Published
2022
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39. Identification of novel biofilm genes in avian pathogenic Escherichia coli by Tn5 transposon mutant library

Author

Hu, Jiangang, primary, Gu, Yi, additional, Lu, Huiqi, additional, Raheem, Muhammad Akmal, additional, Yu, Fangheng, additional, Niu, Xiangpeng, additional, Zuo, Jiakun, additional, Yin, Huifang, additional, Huang, Cuiqin, additional, Song, Xiangjun, additional, Tu, Jian, additional, Zhou, Wen, additional, Jiang, Wei, additional, Chen, Zhaoguo, additional, Han, Xiangan, additional, and Qi, Kezong, additional

Published
2022
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50. Effect of Disordered Gut Microbiota on Serum Metabolome Alterations in Colorectal Tumor-Bearing Mice Under The Intervention of Fufangchangtai

Author

Shilan Zhu, Xiaoyu Wang, Jie-Ge Huo, Mengmeng Cai, Xiaoyu Chen, Jia-Lin Gu, Zhibing Lin, Yu-Zhu Ma, Chen Zhaoguo, Ya Xiao, and Jinmiao Lu

Subjects
biology, business.industry, Intervention (counseling), Metabolome, Medicine, Gut flora, biology.organism_classification, business, Bioinformatics, Colorectal tumor
Abstract

Background: The occurrence and development of colorectal cancer is related to the compositional and functional variation of gut microbiota. Compared with healthy people, gut microbiota of patients with colorectal cancer is in disorder. Most traditional Chinese Medicine is effective by oral administration and has both anti-tumor effect and enteric microecological regulative effect. However, whether the dysbiosis of gut microbiota under tumor burden affects the serum metabolome of human body that related to traditional Chinese medicine is unclear. In this study, Fufangchangtai (FFCT) was chosen to be the model prescription to explore the correlation between gut microbiota and the serum metabolism related to FFCT in anti colorectal cancer treatment. Results: The gut microbiota between colorectal tumor-bearing mice and healthy mice were determined by 16S rRNA gene sequencing, showing quite differences between the two groups and suggesting that Firmicutes , Deferribacteres , Bacteroidetes and Proteobacteria were marked differential intestinal bacteria. The alternations in serum metabolome in the FFCT-treating tumor-bearing mice and simple FFCT-treating mice were detected using Liquid chromatogragh-mass spectrometer (LC/MS), showing significant differences between the two groups as well. Metabolites of FFCT like Citric acid, （±）12-HEPE, Cycloartanyl ferulate were much more in simple FFCT-treating mice, indicating that the present of tumor could affect the absorption and metabolism of FFCT. Additionally, these differential metabolites of FFCT involved in multiple pathways including the Alanine, aspartate and glutamate metabolism, Central carbon metabolism in cancer, Biosynthesis of amino acids. Different doses of FFCT were given to the tumor-bearing mice through oral administration, and the results of gut microbiota 16S rRNA gene sequencing showing that FFCT-treating groups has higher abundance of Firmicutes , Turicibacter and Roseburia than tumor-bearing group, moreover, the abundance of these bacteria was positively correlated to the drug concentration. Firmicutes and Bacteroidetes in FFCT-treating groups showed a similar trend with Healthy group, indicating the modulation of FFCT on gut microbiota of colorectal tumor-bearing mice.Conclusions: Collectively, we concluded that the dysbiosis of gut microbiota in tumor-bearing mice could affect the serum metabolome of human body that related to FFCT, and FFCT could correct the gut microbiota of colorectal tumor-bearing mice. It was pointed out that the GM should be concerned during the therapy of FFCT. The more healthier intestinal microenvironment was conductive to the better clinical curative effect.

Published
2021

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