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A mixed H[subscript 2]/H[subscript omega] filter design is described, for multirate transmultiplexer systems with dispersive channel and additive noise.

Abstract Background The cationic peptide antibiotic polymyxin has recently been reevaluated in the treatment of severe infections caused by gram negative bacteria. Methods In this study, the genetic determinants for capsular polysaccharide level and lipopolysaccharide modification involved in polymyxin B resistance of the opportunistic pathogen Klebsiella pneumoniae were characterized. The expressional control of the genes responsible for the resistance was assessed by a LacZ reporter system. The PmrD connector-mediated regulation for the expression of pmr genes involved in polymyxin B resistance was also demonstrated by DNA EMSA, two-hybrid analysis and in vitro phosphor-transfer assay. Results Deletion of the rcsB, which encoded an activator for the production of capsular polysaccharide, had a minor effect on K. pneumoniae resistance to polymyxin B. On the other hand, deletion of ugd or pmrF gene resulted in a drastic reduction of the resistance. The polymyxin B resistance was shown to be regulated by the two-component response regulators PhoP and PmrA at low magnesium and high iron, respectively. Similar to the control identified in Salmonella, expression of pmrD in K. pneumoniae was dependent on PhoP, the activated PmrD would then bind to PmrA to prolong the phosphorylation state of the PmrA, and eventually turn on the expression of pmr for the resistance to polymyxin B. Conclusions The study reports a role of the capsular polysaccharide level and the pmr genes for K. pneumoniae resistance to polymyxin B. The PmrD connector-mediated pathway in governing the regulation of pmr expression was demonstrated. In comparison to the pmr regulation in Salmonella, PhoP in K. pneumoniae plays a major regulatory role in polymyxin B resistance.

Drawing on theories of dramaturgy and environmental psychology, this research intends to (1) develop a more comprehensive framework exploring the links of the marketing drivers and the consequences of hedonic experiences in the context of the leisure services, i.e. Karaoke Houses, (2) examine the effects of three experiential marketing elements (physical environment, employee service, and core service) on hedonic experience outcomes (emotions and word of mouth (WOM) intentions), and (3) test the mediating role of hedonic values (HVs) between the link of emotions and WOM intentions. With 441 valid questionnaires and using structural equation modelling analysis to test the hypotheses, this research found that (1) WOM referral intentions mainly result from both perceived service quality (SQ) and HV, with the former being more important than the latter. (2) While physical environment, employee service and core service simultaneously influence SQ, core service is the most influential determinant. (3) Experiential emotions are evoked by both physical (ambience and facilities) and social servicescapes (interactions with employees), while SQ mediates the relationship between core service and emotions. (4) Perceived HV fully mediates the effect of emotions on WOM. [ABSTRACT FROM AUTHOR]

Background and Purpose 4- Phenylquinolin-2(1 H)-one (4- PQ) derivatives can induce cancer cell apoptosis. Additional new 4- PQ analogs were investigated as more effective, less toxic antitumour agents. Experimental Approach Forty-five 6,7,8-substituted 4-substituted benzyloxyquinolin-2(1 H)-one derivatives were synthesized. Antiproliferative activities were evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliun bromide assay and structure-activity relationship correlations were established. Compounds 9b, 9c, 9e and 11e were also evaluated against the National Cancer Institute-60 human cancer cell line panel. Hoechst 33258 and Annexin V- FITC/ PI staining assays were used to detect apoptosis, while inhibition of microtubule polymerization was assayed by fluorescence microscopy. Effects on the cell cycle were assessed by flow cytometry and on apoptosis-related proteins (active caspase-3, -8 and -9, procaspase-3, -8, -9, PARP, Bid, Bcl-x L and Bcl-2) by Western blotting. Key Results Nine 6,7,8-substituted 4-substituted benzyloxyquinolin-2(1 H)-one derivatives ( 7e, 8e, 9b, 9c, 9e, 10c, 10e, 11c and 11e) displayed high potency against HL-60, Hep3 B, H460, and COLO 205 cancer cells ( IC50 < 1 μ M) without affecting Detroit 551 normal human cells ( IC50 > 50 μ M). Particularly, compound 11e exhibited nanomolar potency against COLO 205 cancer cells. Mechanistic studies indicated that compound 11e disrupted microtubule assembly and induced G2/ M arrest, polyploidy and apoptosis via the intrinsic and extrinsic signalling pathways. Activation of JNK could play a role in TRAIL-induced COLO 205 apoptosis. Conclusion and Implications New quinolone derivatives were identified as potential pro-apoptotic agents. Compound 11e could be a promising lead compound for future antitumour agent development. [ABSTRACT FROM AUTHOR]

Presents information on a study which proposed a mixed H.../H infinity filter design for multirate transmultiplexer systems with dispersive channel and additive noise. State-space description of transmultiplexer systems; Simulation results; Conclusions.

Objectives: Diabetic nephropathy (DN) is one of the most prevalent secondary complications associated with diabetes mellitus. Decades of research have implicated multiple pathways in the etiology and pathophysiology of diabetic nephropathy. There has been no reliable predictive biomarkers for the onset or progression of DN and no successful treatments are available., Methods: In the present study, we explored the datasets of RNA sequencing data from patients with Type II diabetes mellitus (T2DM)-induced nephropathy to identify a novel gene signature. We explored the target bioactive compounds identified from Azanza garckeana , a medicinal plant commonly used by the traditional treatment of diabetes nephropathy., Results: Our analysis identified lymphotoxin beta (LTB), SRY-box transcription factor 4 (SOX4), SOX9, and WAP four-disulfide core domain protein 2 (WFDC2) as novel signatures of T2DM-induced nephropathy. Additional analysis revealed the pathological involvement of the signature in cell-cell adhesion, immune, and inflammatory responses during diabetic nephropathy. Molecular docking and dynamic simulation at 100 ns conducted studies revealed that among the three compounds, Terpinen-4-ol exhibited higher binding efficacies (binding energies (ΔG) = -3.9~5.5 kcal/mol) against the targets. The targets, SOX4, and SOX9 demonstrated higher druggability towards the three compounds. WFDC2 was the least attractive target for the compounds., Conclusion: The present study was relevant in the diagnosis, prognosis, and treatment follow up of patients with diabetes induced nephropathy. The study provided an insight into the therapeutic application of the bioactive principles from Azanza garckeana . Continued follow-up invitro validations study are ongoing in our laboratory., Competing Interests: None., (AJTR Copyright © 2023.)