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23 results on '"Chen, Rong-Feng"'

10. A genetic linkage map of cucumber (Cucumis sativus L) combining SRAP and ISSR markers

Author

Guo-Hua Liang, Chen Rong Feng, Martin A. Yeboah, Minghong Gu, and Chen Xuehao

Subjects
Genetics, biology, Genetic linkage map, Marker-assisted selection, Quantitative trait locus, biology.organism_classification, Applied Microbiology and Biotechnology, SRAP, ISSR, linkage mapping, cucumber, Inbred strain, Genetic linkage, Genetic marker, F2 population, Agronomy and Crop Science, Molecular Biology, Cucumis, Biotechnology
Abstract

Sequence-related amplified polymorphism (SRAP) and Inter-simple sequence repeat (ISSR) markers are both simple and efficient maker systems adapted to many crops and for multiple purposes. In this study a genetic map based on SRAP and ISSR markers was constructed for cucumber (Cucumis sativus L.) based on the segregations of SRAP and ISSR markers in 112 plants of F2 population derived from a cross between two cucumber inbred lines PW0832 and PW0801. In the investigation of polymorphisms with 50 ISSR primers and 132 SRAP primer combinations, 13 (26%) ISSR primers and 26 (20%) SRAP primer pairs were polymorphic generating a total of 109 polymorphic markers of which 48 were ISSR and 61 were SRAP. The average polymorphic bands were four for ISSR and two for SRAP. All the 109 polymorphic markers were scored for segregation of which 86 satisfied the Mandelian segregation ratio of 3:1. These data were used to construct an integrated linkage map for cucumber consisting of 62 loci, distributed in seven linkage groups (LGs) spanning a total of 992.2 cM, with an average distance of 16.0 cM between two adjacent loci. These markers would be very useful tool for marker assisted selection in cucumber breeding as well as for studies in quantitative traits.

Published
2007
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23. [Synthesis and antibacterial activity of 7-(4-acylamino-thiocarbamoyl-1-piperazinyl) fluoroquinolone analogues in vitro].

Author

Qu LB, Tian MC, Cheng SX, and Chen RF

Subjects
Anti-Infective Agents chemistry, Anti-Infective Agents pharmacology, Bacillus subtilis drug effects, Ciprofloxacin chemistry, Ciprofloxacin pharmacology, Combinatorial Chemistry Techniques methods, Escherichia coli drug effects, Fluoroquinolones chemistry, Fluoroquinolones pharmacology, Microbial Sensitivity Tests, Molecular Structure, Norfloxacin chemistry, Norfloxacin pharmacology, Anti-Infective Agents chemical synthesis, Fluoroquinolones chemical synthesis
Abstract

Aim: To synthesize new fluoroquinolone analogues as antibacterial compounds., Methods and Results: By reaction of acryl chloride(chloro-carbonic ester) with sodium sulfocyanate, acyl isosulfocyanic ester were easily obtained. Twelve 7-(4-acylamino-thiocarbamoyl-1-piperazinyl) fluoroquinolone analogues (1-12) were synthesized through modifying the 7-piperazine of norflorxacin and ciprofloxacin with isosulfocyanic ester synthesized above. The structures of synthesized compounds were characterized by 1HNMR, IR and elemental analysis., Conclusion: Antibacterial activities of the new compounds were evaluated in vitro compared with norflorxacin. Compounds 5, 7, 10 and 12 showed antibacterial activities.

Published
2003

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