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39 results on '"Chen, Jyh-Jian"'

6. A radiation heating-based oscillatory polymerase chain reaction system for detecting donkey-hide gelatin

Author

Chen, Jyh Jian, Kuo, Tsung Yueh, Sheu, Shyang Chwen, and Song, Yi Syuan

Abstract

Polymerase chain reaction (PCR) is a biochemical technique for copying DNA by repeatedly changing the temperature of nucleic acid samples. In this study, we aim to create an oscillatory PCR system with a short reaction time, which could have significant practical implications. The device uses an electromechanical module with a servo motor and a homemade heating-cooling system that combines a halogen lamp, a Peltier element, a cooling fin, and a blower fan. We code the motor program to control the reaction chamber moving back and forth in the infrared thermal cycling system. The system uses one infrared lamp for heating and one Peltier element/thermal dissipation fins/blower fan for cooling to shorten the overall reaction time of the thermal process. Results show that using the radiant heating and convection cooling method and a micro-sample of 10 μL to perform a PCR, the total time spent is 35 minutes, which saves about 1 hour compared to commercially available PCR instruments. The proposed PCR approach could specifically detect donkey-hide gelatin (DHG) made from donkey skin, offering a rapid and cost-effective solution. Therefore, our device has the advantages of easy manufacturing, low cost, and rapid temperature ramping rate for PCR.

Published
2024
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19. A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification

Author

Wongniyom, Arayan, Chen, Jyh-Jian, Wongniyom, Arayan, and Chen, Jyh-Jian

Abstract

The gene amplification technique is used in medical science and biotechnology popularly. The loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification method that can be utilized in low-resource settings and does not require expensive or complex instruments. The annealing process of LAMP is performed at a constant temperature (around 65 °C) for an hour. From previous works, phase change materials (PCMs) have been applied for the latent heat energy storages due to their isothermal operation during phase transitions. The utilization of the melting temperature of the paraffin wax to maintain the suitable temperature for LAMP process is introduced in our study. To enhance the heat transfer inside the energy storage unit, several pin fins are constructed on the inner surface of the unit which is 50 mm × 50 mm × 50 mm in inner size. The influence of various numbers of pin fins on the temperature distribution is examined by the numerical simulation. The heat transfer inside the paraffin enclosure with fins is better than that without fin. It can be found that the temperature uniformity in the PCM inside the enclosure with fins shows much better. Though the numbers of the fin are increased, some transfer energy from the heat source remains at the enclosure wall and cannot penetrate into the PCM. The duration for all PCM melted from solid phase to liquid phase is over 10 minutes. In the future, the geometric parameters on the duration for phase transition of PCM inside the LAMP chamber will be investigated.

Published
2017

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