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The inline gas-liquid cyclone separator has been gradually used in oil & gas industry due to its high separation efficiency and less footprint. However, under the inlet flow conditions with large range of gas volume fractions, the research on structure design and separation performance are seriously insufficient. The preliminary structural design of the inline gas-liquid separator was carried out according to the particle separation theory, and the key structural parameters were optimized by using the Response Surface Method (RSM) together with Computational Fluid Dynamics (CFD). The structure optimization has improved the degassing efficiency by 17.44% and dehydration efficiency by 7.59%. With air and water as working medium, the separation performance of the inline gas-liquid cyclone separator was experimentally investigated in laboratory under a large range of gas volume fractions. The results show that the separation efficiency for both degassing and dehydration can exceed 80% with the inlet gas volume fraction ranges from 0.2 to 0.8. The optimum normalized flow split and liquid volume flow rate are 0.9 –1.2 and 8 m3/h, respectively. [ABSTRACT FROM AUTHOR]

Background: Most arthropods are famous for their large reproductive capacity, with the ovary playing a vital role in the process. The study of the regulatory mechanisms of ovarian development may have the potential for a reproduction‐based pest management strategy. GATA‐binding transcription factors (GATAs) as important regulatory factors mediate many physiological processes, including development, immunity, insecticide resistance and reproduction. The Pannier (pnr), a member of GATA family, was confirmed to be involved in ovarian development of Bactrocera dorsalis in our previous study. However, the direct evidence of pnr regulating the fly ovarian development is still lacking. Results: We used CRISPR/Cas9 to create Bdpnr loss‐of‐function mutations. Homozygous Bdpnr−/− mutants were nonviable, with most individuals dying during embryogenesis, some surviving to the larval stages, and the remaining few dying during pupation. In contrast, heterozygous individuals reached the adult stage, but ovarian development was disrupted, with concomitant decreases in egg laying and hatching rates. We also found that two genes encoding vitellogenin proteins (BdVg1 and BdVg2) and the vitellogenin receptor (BdVgR) were significantly down‐regulated in heterozygous mutants compared to wild‐type controls. Conclusion: These results indicate that Bdpnr is required for embryonic and post‐embryonic development, including the formation of ovaries. Bdpnr could therefore be considered as a molecular target for tephritid fly pest control. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Background: The negative feedback circuit NIK-SIN could inhibit the systemic inflammation and protect mouse from endotoxic shock. However, the physiological significance of NIK-SIX feedback circuit in the maintenance of intestinal immune homeostasis and prevention of early-onset spontaneous colitis is not known., Objective: To explore the role of NIK-SIX axis in the maintenance of intestinal immune homeostasis., Methods: The conditional knockout of NIK encoding gene, Map3k14, in the Cd11c+ dendritic cells were generated by crossing Map3k14-flox mice with Cd11c-Cre mice. DSS was used for colitis models. The expression of cytokines in the intestinal immune cells, isolated from Map3k14-cKO mice were detected by qPCR. The siRNA molecules were used for the silencing of SIN-proteins. Then luciferase assays and chromatin immunoprecipitation combined with qPCR were applied for mechanism investigations., Results: The expression of SIX1 and SIX2 protein in BMDMs from WT were significantly lower than in the Map3k14-cKO mice. In vitro, the NIK-/- human-derived circulating monocytes also failed to express SIX-proteins under the stimulation of non-canonical NF-κB agonists. The expression of cytokines was significantly decreased in human circulating monocytes with overexpression SIN-proteins. The expression of cytokines in macrophages, DCs and T cells isolated from Map3k14-cKO mice were significantly increased in the DSS-induced models. Higher expression of cytokines was observed in the SIN1-/- and SIN2-/- cells including human circulating monocytes, mouse-derived BMDMs, intestinal macrophages and DCs. SIN-proteins directly bound the promoter region of inflammatory genes., Conclusion: NIK-SIX axis down-regulated inflammatory gene expression and plays a pivotal role in the maintenance of intestinal immune homeostasis.