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Your search keyword '"Chen, Gong-Xing"' showing total 7 results
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7 results on '"Chen, Gong-Xing"'

5. Design and implementation of path planning for cleaning robot

Author

Chen Chui-xin, Chen Gong-xing, and Chen Chao-da

Subjects
Computer science, Real-time computing, Path (graph theory), Robot, Context (language use), Mobile robot, Motion planning, Coverage map, Simulation, Mobile robot navigation, Robot control
Abstract

The design of the main use of lightweight car to complete the cleaning robot path planning in reality, detection by infrared detection head, can be self completed indoor cleaning line. The robot through the infrared probe sensing the environment information, the establishment of the coverage map of the environment, and in the process of movement on the map update. On the other hand, in order to ensure the complete coverage of all areas at the same time, reduce the duplication of coverage, improve work efficiency, this paper puts forward square, saw form covering algorithm and spiral coverage algorithm, the three algorithms do not need complicated mathematical calculation, to ensure the real time of the algorithm, which reduces the memory requirements, at the same time, the algorithm does not make the entire search space and avoid the defects of the traditional grid algorithm as the space enlarges the real-time rapid decline.This topic is in this context, research on the path to cover more ground area is more important. Cleaning robot path planning there are many difficulties in application and design, such as low coverage, high repetition rate, so that the work efficiency is not high, so the subject has practical significance.

Published
2015

6. [Knockdown of PLK1 mRNA by special siRNA].

Author

Chen GX, Dong QH, Zhang JW, Fu FF, Xu ZF, Liang QY, Zheng S, and Ding JY

Subjects
Blotting, Western, Cell Line, Tumor, Humans, Polymerase Chain Reaction, RNA, Small Interfering physiology, Transfection, Polo-Like Kinase 1, Cell Cycle Proteins genetics, Gene Knockdown Techniques methods, Protein Serine-Threonine Kinases genetics, Proto-Oncogene Proteins genetics, RNA, Messenger genetics, RNA, Small Interfering genetics
Abstract

To study knockdown effect of small interfering RNA (siRNA) to PLK1 (Polo-like kinase 1) mRNA in colorectal cancer cell line SW480 and its mitosis and growth was changed. Ten special siRNA molecules were designed targeting different sites of PLK1 mRNA sequence and chemically synthesized. The siRNA molecules were transfected into SW480 by Oligofectamine. The gene mRNA level was assayed by Real-Time PCR. The changes of PLK1 protein, SW480 cell cycle and survival percentage was checked by Western-blot, Flow cytometry and Cell counter assays respectively. All 10 siRNA molecules knocked PLK1 mRNA down in different level. Of them P1, P4 and P9 showed over 80% knockdown efficiency and the others had more than 20% knockdown efficiency to PLK1 mRNA. The best knockdown effect over 95% of all groups was at 25 nmol/L of a mixture with P1, P4 and P9 siRNA equally. In this situation the protein was very less and the cells were blocked at G2 phase of cell cycle. After 72 h cell survival percentages were consistent with PKL1 mRNA level change by siRNA gradient concentration. The results showed that siRNA targeting PLK1 mRNA had effectively knocked PLK1 mRNA down in SW480 cell line. And a blended siRNAs held the best knockdown effect. The cell was blocked on the mitosis and growth.

Published
2006

7. [Study of an enhancer in promoter region of human colorectal cancer related ST13 gene].

Author

Chen GX, Zhang JW, and Zheng S

Subjects
Base Sequence, Genes, Reporter, Humans, Molecular Sequence Data, Transcription, Genetic, Transfection, Carrier Proteins genetics, Colorectal Neoplasms genetics, Enhancer Elements, Genetic genetics, Promoter Regions, Genetic genetics, Tumor Suppressor Proteins genetics
Abstract

Objective: To study the base sequence of an enhancer in up-stream 5'-flank near regulation region (from -595 to +74) of human colorectal cancer related gene ST13., Methods: Several deletion PCR primers were designed. Amplified DNA fragments of ST13 gene 5'-flank near region were cloned with pGEMT-EASY vector and sequenced; then subcloned into several pGL2 report vectors respectively. Equal quantitative recombined DNA was transfected into SW620 cell lines and the luciferase activity was checked., Results: Several amplified base sequence fragments (669 bp,263 bp,163 bp) in pGL2-Basic all enhanced and promoted luciferase gene expression strongly. The 47 bp and 101 bp fragments didn't promote luciferase gene expression. 101 bp fragment recombined with pGL2-Promoter enhanced luciferase gene expression distinctly (P<0.01), but the effect was less strong than the positive pGL2-Control(P<0.05)., Conclusion: The base sequence 101 bp (from -595 to -494) in up-stream 5'-flank near regulation region of colorectal cancer related gene ST13 is an enhancer regulating gene transcript.

Published
2004
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