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Collagenase digestion (d) and cellular outgrowth (og) are the current modalities of meniscus fibrochondrocytes (MFC) isolation for bioengineering and mechanobiology‐related studies. However, the impact of these modalities on study outcomes is unknown. Here, we show that og‐ and d‐isolated MFC have distinct proliferative capacities, transcriptomic profiles via RNA sequencing (RNAseq), extracellular matrix (ECM)‐forming, and migratory capacities. Our data indicate that microtissue pellet models developed from og‐isolated MFC display a contractile phenotype with higher expressions of alpha‐smooth muscle actin (ACTA2) and transgelin (TAGLN) and are mechanically stiffer than their counterparts from d‐MFC. Moreover, we introduce a novel method of MFC isolation designated digestion‐after‐outgrowth (dog). The transcriptomic profile of dog‐MFC is distinct from d‐ and og‐MFC, including a higher expression of mechanosensing caveolae‐associated caveolin‐1 (CAV1). Additionally, dog‐MFC were superior chondrogenically and generated larger‐size microtissue pellet models containing a higher frequency of smaller collagen fibre diameters. Thus, we demonstrate that the modalities of MFC isolation influence the downstream outcomes of bioengineering and mechanobiology‐related studies. [ABSTRACT FROM AUTHOR]

This review paper investigates the nuanced interplay between employment status and various dimensions of psychological well-being among females. Focusing on self-esteem, selfefficacy, mental health, and marital adjustment, the study delves into the comparative analysis between working and non-working women. Employing a systematic approach, existing literature is scrutinized to elucidate the differential impacts of employment on these key domains. The findings suggest that employment status significantly influences selfesteem, with working women generally exhibiting higher levels. Moreover, self-efficacy tends to be bolstered by workforce participation, contributing to a sense of agency and mastery in various life domains. Mental health outcomes reveal a complex relationship, with employment offering both protective and risk factors depending on individual circumstances and societal contexts. Furthermore, marital adjustment emerges as a critical dimension affected by employment status, with implications for relational dynamics and family functioning. By synthesizing empirical evidence, this review underscores the multifaceted implications of employment for the psychological well-being of women, providing insights for future research and interventions aimed at enhancing women's holistic empowerment and fulfilment. [ABSTRACT FROM AUTHOR]

Corneal scarring is the major source of permanent blindness worldwide. The complex pathophysiology of corneal scarring is not comprehensibly understood as it involves the interaction of a constellation of pro‐fibrotic cytokines influencing several signaling pathways involved in corneal scar development. In the present study, an attempt has been made to generate a relatively simple in vitro corneal scar model using primary corneal keratocytes by exogenously providing an optimized dose of combination of cytokines (TGF‐β1, IL‐6, and IL‐8) involved in scar formation in situ. Data obtained from gene and protein expression analysis depicted enhanced ECM production with discrete expression of myofibroblast specific markers. The protein–protein interactions associated these proteins to various pathways involved in wound healing, cellular migration, and cytoskeletal remodeling justifying high relevance to in vivo scar formation. Hence the developed model can be used to acquire understanding about corneal scar pathophysiology and thus might be useful for designing the treatment modalities and efficacies for controlling scar formation. [ABSTRACT FROM AUTHOR]