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4. Different Biomechanical Cell Behaviors in an Epithelium Drive Collective Epithelial Cell Extrusion.

Author

Balasubramaniam, Lakshmi, Jain, Shreyansh, Dang, Tien, Lagoutte, Emilie, Marc Mège, René, Chavrier, Philippe, Ladoux, Benoit, and Rossé, Carine

Subjects
CELLULAR mechanics, PROTEIN kinase C, MAMMARY glands, TISSUE mechanics, MORPHOGENESIS
Abstract

In vertebrates, many organs, such as the kidney and the mammary gland form ductal structures based on the folding of epithelial sheets. The development of these organs relies on coordinated sorting of different cell lineages in both time and space, through mechanisms that remain largely unclear. Tissues are composed of several cell types with distinct biomechanical properties, particularly at cell‐cell and cell‐substrate boundaries. One hypothesis is that adjacent epithelial layers work in a coordinated manner to shape the tissue. Using in vitro experiments on model epithelial cells, differential expression of atypical Protein Kinase C iota (aPKCi), a key junctional polarity protein, is shown to reinforce cell epithelialization and trigger sorting by tuning cell mechanical properties at the tissue level. In a broader perspective, it is shown that in a heterogeneous epithelial monolayer, in which cell sorting occurs, forces arising from epithelial cell growth under confinement by surrounding cells with different biomechanical properties are sufficient to promote collective cell extrusion and generate emerging 3D organization related to spheroids and buds. Overall, this research sheds light on the role of aPKCi and the biomechanical interplay between distinct epithelial cell lineages in shaping tissue organization, providing insights into the understanding of tissue and organ development. [ABSTRACT FROM AUTHOR]

Published
2024
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5. Metastasis-suppressor NME1 controls the invasive switch of breast cancer by regulating MT1-MMP surface clearance

Author

Lodillinsky, Catalina, Fuhrmann, Laetitia, Irondelle, Marie, Pylypenko, Olena, Li, Xiao-Yan, Bonsang-Kitzis, Hélène, Reyal, Fabien, Vacher, Sophie, Calmel, Claire, De Wever, Olivier, Bièche, Ivan, Lacombe, Marie-Lise, Eiján, Ana Maria, Houdusse, Anne, Vincent-Salomon, Anne, Weiss, Stephen J., Chavrier, Philippe, and Boissan, Mathieu

Published
2021
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7. Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling

Author

Boissan, Mathieu, Montagnac, Guillaume, Shen, Qinfang, Griparic, Lorena, Guitton, Jérôme, Romao, Maryse, Sauvonnet, Nathalie, Lagache, Thibault, Lascu, Ioan, Raposo, Graça, Desbourdes, Céline, Schlattner, Uwe, Lacombe, Marie-Lise, Polo, Simona, van der Bliek, Alexander M, Roux, Aurélien, and Chavrier, Philippe

Subjects
Underpinning research, 1.1 Normal biological development and functioning, Adenosine Triphosphate, Animals, Cell Line, Cell Membrane, Coated Pits, Cell-Membrane, Dynamins, Endocytosis, GTP Phosphohydrolases, Guanosine Diphosphate, Guanosine Triphosphate, Humans, Intracellular Membranes, Membrane Fusion, Mitochondria, NM23 Nucleoside Diphosphate Kinases, Nucleoside Diphosphate Kinase D, General Science & Technology
Abstract

Dynamin superfamily molecular motors use guanosine triphosphate (GTP) as a source of energy for membrane-remodeling events. We found that knockdown of nucleoside diphosphate kinases (NDPKs) NM23-H1/H2, which produce GTP through adenosine triphosphate (ATP)-driven conversion of guanosine diphosphate (GDP), inhibited dynamin-mediated endocytosis. NM23-H1/H2 localized at clathrin-coated pits and interacted with the proline-rich domain of dynamin. In vitro, NM23-H1/H2 were recruited to dynamin-induced tubules, stimulated GTP-loading on dynamin, and triggered fission in the presence of ATP and GDP. NM23-H4, a mitochondria-specific NDPK, colocalized with mitochondrial dynamin-like OPA1 involved in mitochondria inner membrane fusion and increased GTP-loading on OPA1. Like OPA1 loss of function, silencing of NM23-H4 but not NM23-H1/H2 resulted in mitochondrial fragmentation, reflecting fusion defects. Thus, NDPKs interact with and provide GTP to dynamins, allowing these motor proteins to work with high thermodynamic efficiency.

Published
2014

9. αTAT1 catalyses microtubule acetylation at clathrin-coated pits.

Author

Montagnac, Guillaume, Meas-Yedid, Vannary, Irondelle, Marie, Castro-Castro, Antonio, Franco, Michel, Shida, Toshinobu, Nachury, Maxence V, Benmerah, Alexandre, Olivo-Marin, Jean-Christophe, and Chavrier, Philippe

Subjects
Hela Cells, Coated Pits, Cell-Membrane, Microtubules, Humans, Tubulin, Acetyltransferases, Adaptor Protein Complex 2, Clathrin, Cell Movement, Protein Binding, Acetylation, Biocatalysis, HeLa Cells, 1.1 Normal biological development and functioning, 2.1 Biological and endogenous factors, Coated Pits, Cell-Membrane, General Science & Technology
Abstract

In most eukaryotic cells microtubules undergo post-translational modifications such as acetylation of α-tubulin on lysine 40, a widespread modification restricted to a subset of microtubules that turns over slowly. This subset of stable microtubules accumulates in cell protrusions and regulates cell polarization, migration and invasion. However, mechanisms restricting acetylation to these microtubules are unknown. Here we report that clathrin-coated pits (CCPs) control microtubule acetylation through a direct interaction of the α-tubulin acetyltransferase αTAT1 (refs 8, 9) with the clathrin adaptor AP2. We observe that about one-third of growing microtubule ends contact and pause at CCPs and that loss of CCPs decreases lysine 40 acetylation levels. We show that αTAT1 localizes to CCPs through a direct interaction with AP2 that is required for microtubule acetylation. In migrating cells, the polarized orientation of acetylated microtubules correlates with CCP accumulation at the leading edge, and interaction of αTAT1 with AP2 is required for directional migration. We conclude that microtubules contacting CCPs become acetylated by αTAT1. In migrating cells, this mechanism ensures the acetylation of microtubules oriented towards the leading edge, thus promoting directional cell locomotion and chemotaxis.

Published
2013

11. Force Mapping in Epithelial Cell Migration

Author

du Roure, Olivia, Saez, Alexandre, Buguin, Axel, Austin, Robert H., Chavrier, Philippe, Siberzan, Pascal, Ladoux, Benoit, and de Gennes, Pierre-Gilles

Published
2005

12. Codependencies of mTORC1 signaling and endolysosomal actin structures

Author

Priya, Amulya, primary, Antoine-Bally, Sandra, additional, Macé, Anne-Sophie, additional, Monteiro, Pedro, additional, Sabatet, Valentin, additional, Remy, David, additional, Dingli, Florent, additional, Loew, Damarys, additional, Demetriades, Constantinos, additional, Gautreau, Alexis M., additional, and Chavrier, Philippe, additional

Published
2023
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16. Coronin 1C promotes triple-negative breast cancer invasiveness through regulation of MT1-MMP traffic and invadopodia function

Author

Castagnino, Alessia, Castro-Castro, Antonio, Irondelle, Marie, Guichard, Alan, Lodillinsky, Catalina, Fuhrmann, Laetitia, Vacher, Sophie, Agüera-González, Sonia, Zagryazhskaya-Masson, Anna, Romao, Maryse, El Kesrouani, Carole, Noegel, Angelika A., Dubois, Thierry, Raposo, Graça, Bear, James E., Clemen, Christoph S., Vincent-Salomon, Anne, Bièche, Ivan, and Chavrier, Philippe

Published
2018
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18. AMOTL1 Promotes Breast Cancer Progression and Is Antagonized by Merlin

Author

Couderc, Christophe, Boin, Alizée, Fuhrmann, Laetitia, Vincent-Salomon, Anne, Mandati, Vinay, Kieffer, Yann, Mechta-Grigoriou, Fatima, Del Maestro, Laurence, Chavrier, Philippe, Vallerand, David, Brito, Isabelle, Dubois, Thierry, De Koning, Leanne, Bouvard, Daniel, Louvard, Daniel, Gautreau, Alexis, and Lallemand, Dominique

Published
2016
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20. Supplementary Figure 7 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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21. Supplementary Figure 5 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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22. Supplementary Movie 1 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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24. Data from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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25. Supplementary Figure Legends 1-11, Methods from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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26. Supplementary Movie 2 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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27. Supplementary Figure 3 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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35. Supplementary Figure 11 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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36. Supplementary Figure 4 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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38. Supplementary Figure 8 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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39. Supplementary Figure 6 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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40. Supplementary Figure 9 from Implication of Metastasis Suppressor NM23-H1 in Maintaining Adherens Junctions and Limiting the Invasive Potential of Human Cancer Cells

Author

Boissan, Mathieu, primary, De Wever, Olivier, primary, Lizarraga, Floria, primary, Wendum, Dominique, primary, Poincloux, Renaud, primary, Chignard, Nicolas, primary, Desbois-Mouthon, Christèle, primary, Dufour, Sylvie, primary, Nawrocki-Raby, Béatrice, primary, Birembaut, Philippe, primary, Bracke, Marc, primary, Chavrier, Philippe, primary, Gespach, Christian, primary, and Lacombe, Marie-Lise, primary

Published
2023
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43. Force tuning through regulation of clathrin-dependent integrin endocytosis

Author

Kyumurkov, Alexander, primary, Bouin, Anne-Pascale, additional, Boissan, Mathieu, additional, Manet, Sandra, additional, Baschieri, Francesco, additional, Proponnet-Guerault, Mathilde, additional, Balland, Martial, additional, Destaing, Olivier, additional, Régent-Kloeckner, Myriam, additional, Calmel, Claire, additional, Nicolas, Alice, additional, Waharte, François, additional, Chavrier, Philippe, additional, Montagnac, Guillaume, additional, Planus, Emmanuelle, additional, and Albiges-Rizo, Corinne, additional

Published
2022
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46. Control of MT1-MMP transport by atypical PKC during breast-cancer progression

Author

Rossé, Carine, Lodillinsky, Catalina, Fuhrmann, Laetitia, Nourieh, Maya, Monteiro, Pedro, Irondelle, Marie, Lagoutte, Emilie, Vacher, Sophie, Waharte, François, Paul-Gilloteaux, Perrine, Romao, Maryse, Sengmanivong, Lucie, Linch, Mark, van Lint, Johan, Raposo, Graça, Vincent-Salomon, Anne, Bièche, Ivan, Parker, Peter J., and Chavrier, Philippe

Published
2014

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