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Your search keyword '"Chaubard, Jean-Luc"' showing total 10 results
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10 results on '"Chaubard, Jean-Luc"'

1. Immunomodulatory fecal metabolites are associated with mortality in COVID-19 patients with respiratory failure

Author

Stutz, Matthew R., Dylla, Nicholas P., Pearson, Steven D., Lecompte-Osorio, Paola, Nayak, Ravi, Khalid, Maryam, Adler, Emerald, Boissiere, Jaye, Lin, Huaiying, Leiter, William, Little, Jessica, Rose, Amber, Moran, David, Mullowney, Michael W., Wolfe, Krysta S., Lehmann, Christopher, Odenwald, Matthew, De La Cruz, Mark, Giurcanu, Mihai, Pohlman, Anne S., Hall, Jesse B., Chaubard, Jean-Luc, Sundararajan, Anitha, Sidebottom, Ashley, Kress, John P., Pamer, Eric G., and Patel, Bhakti K.

Published
2022
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4. Cosmetic Potential of a Recombinant 50 kDa Protein

Author

Aly, Nesma, primary, Benoit, Emilie, additional, Chaubard, Jean-Luc, additional, Chintalapudi, Kavyasree, additional, Choung, Soojin, additional, de Leeuw, Monique, additional, Diaz, Matthew, additional, Dueppen, Dan, additional, Ferraro, Bryce, additional, Fischetti, Valerie, additional, Gassaway, Evan, additional, Hansenne-Cervantes, Isabelle, additional, Heeres, Arjan, additional, Karas, Christina, additional, Khan, Mohamed, additional, Kral, Jonathan M., additional, Lam, Srujana, additional, Lartey, Richel, additional, Leonard, Mencius, additional, Lue, Stanley W., additional, McDaniel, Joshua, additional, Ramirez, Kevin, additional, Rauw, Brenna, additional, Raymond, Kelly A., additional, Roggero-Lovisi, Catherine, additional, Rubin, Scott, additional, Ruebling-Jass, Kristin, additional, Spiegelhoff, Zoë, additional, Stewart, Monica Celise, additional, Vajpeyi, Shashwat, additional, Vicente, Alejandro, additional, Vincent, Kathleen E., additional, Wang, Jing, additional, Williamson, David, additional, Yu, Zhihao, additional, and Dai, Lixin, additional

Published
2022
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6. Inhibiting antibiotic-resistant Enterobacteriaceae by microbiota-mediated intracellular acidification

Author

Sorbara, Matthew T., primary, Dubin, Krista, additional, Littmann, Eric R., additional, Moody, Thomas U., additional, Fontana, Emily, additional, Seok, Ruth, additional, Leiner, Ingrid M., additional, Taur, Ying, additional, Peled, Jonathan U., additional, van den Brink, Marcel R.M., additional, Litvak, Yael, additional, Bäumler, Andreas J., additional, Chaubard, Jean-Luc, additional, Pickard, Amanda J., additional, Cross, Justin R., additional, and Pamer, Eric G., additional

Published
2018
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7. Development of Chemoenzymatic Labeling Approaches for the Detection of Fucosylated Biomarkers

Author

Chaubard, Jean-Luc

Subjects
Chemistry, Chemoenzymatic Labeling, Glycan Detection, Fucose
Abstract

Protein fucosylation regulates a diverse set of physiological functions such as memory and learning, development, and disease pathogenesis. However, our current understanding of these processes is far behind that of other post-translational modifications, such as phosphorylation. This is, in part, due to the lack of tools available for the study of this important protein modification. To address this need, I have developed novel chemoenzymatic methods that enable the labeling and detection of unique forms of fucosylation, specifically fucose-α(1-2)-galactose (Fucα(1-2)Gal) and core fucose. Additionally, novel glycosyltransferase assays were developed in-house to aid in the future development of both new and existing chemoenzymatic approaches. I have demonstrated that the approach to detect Fucα(1-2)Gal is highly selective for this disaccharide motif, detects a variety of complex glycans and glycoproteins, and can be used to profile the relative abundance of this motif on live cells, discriminating malignant from normal cells. I have also shown that the chemoenzymatic detection of core fucose exhibits superior specificity towards this glycan on a variety of complex N-glycans and when compared to current fucose-specific lectins. Further, the approach is amenable to detection of core fucosylated glycans from multiple biological settings, can be exploited as an antibody-conjugation method, and can be integrated into a diagnostic platform for the profiling of protein specific core fucosylation levels. These approaches represent new potential strategies for biomarker identification and expand the technologies available for understanding the role of these important fucosylated glycans in physiology and disease.

Published
2016
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8. Inhibiting antibiotic-resistant Enterobacteriaceae by microbiota-mediated intracellular acidification

Author

Sorbara, Matthew T., Dubin, Krista, Littmann, Eric R., Moody, Thomas U., Fontana, Emily, Seok, Ruth, Leiner, Ingrid M., Taur, Ying, Peled, Jonathan U., van den Brink, Marcel R.M., Litvak, Yael, Bäumler, Andreas J., Chaubard, Jean-Luc, Pickard, Amanda J., Cross, Justin R., and Pamer, Eric G.

Abstract

Klebsiella pneumoniae, Escherichia coli, and other members of the Enterobacteriaceae family are common human pathogens that have acquired broad antibiotic resistance, rendering infection by some strains virtually untreatable. Enterobacteriaceae are intestinal residents, but generally represent <1% of the adult colonic microbiota. Antibiotic-mediated destruction of the microbiota enables Enterobacteriaceae to expand to high densities in the colon, markedly increasing the risk of bloodstream invasion, sepsis, and death. Here, we demonstrate that an antibiotic-naive microbiota suppresses growth of antibiotic-resistant clinical isolates of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis by acidifying the proximal colon and triggering short chain fatty acid (SCFA)–mediated intracellular acidification. High concentrations of SCFAs and the acidic environment counter the competitive edge that O2 and NO3 respiration confer upon Enterobacteriaceae during expansion. Reestablishment of a microbiota that produces SCFAs enhances clearance of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis from the intestinal lumen and represents a potential therapeutic approach to enhance clearance of antibiotic-resistant pathogens.

Published
2019
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9. Chemoenzymatic approaches to the imaging and detection of cancer relevant fucosylated glycoconjugates

Author

Chaubard, Jean-Luc, Krishnamurthy, Chithra, Ban, Lan, Yi, Wen, Smith, David F., Wilson, Iain B., Hsieh-Wilson, Linda C., Chaubard, Jean-Luc, Krishnamurthy, Chithra, Ban, Lan, Yi, Wen, Smith, David F., Wilson, Iain B., and Hsieh-Wilson, Linda C.

Abstract

A major focus in the field of glycomics has been the development of new strategies for the detection and quantification of glycans and glycoconjugates. With alterations in glycoconjugate structure being a hallmark of various cancers, these strategies can discover new cancer biomarkers and be developed into new clin. diagnostic tools. Here we report chemoenzymic strategies for the rapid, sensitive detection of cancer-relevant fucosylated glycoconjugates. Our methods exploit non-mammalian glycosyltransferases that accept non-natural donor substrates. We then use "Click" chem. to append reporter tags for the detection of these glycans. We have developed methods for the detection of glycans contg. fucoseα(1-2)galactose (Fucα(1-2) Gal), a motif implicated cancer pathogenesis, as well as core fucosylated glycans, a carbohydrate modification that is upregulated in various cancer states and mediates cell signaling events. We demonstrate the specificity and utility of these methods for the detection of cancerous compared to healthy cells and tissues.

Published
2013

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