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2. Consensus statement from the first RdRp Summit: advancing RNA virus discovery at scale across communities.

Author

Charon, Justine, Olendraite, Ingrida, Forgia, Marco, Li Chuin Chong, Hillary, Luke S., Roux, Simon, Kupczok, Anne, Debat, Humberto, Sakaguchi, Shoichi, Tahzima, Rachid, Nakagawa, So, Babaian, Artem, Abroi, Aare, Bejerman, Nicolas, Mansour, Karima Ben, Brown, Katherine, Butkovic, Anamarija, Cervera, Amelia, Charriat, Florian, and Guowei Chen

Subjects
RNA viruses, RNA replicase
Abstract

Improved RNA virus understanding is critical to studying animal and plant health, and environmental processes. However, the continuous and rapid RNA virus evolution makes their identification and characterization challenging. While recent sequence-based advances have led to extensive RNA virus discovery, there is growing variation in how RNA viruses are identified, analyzed, characterized, and reported. To this end, an RdRp Summit was organized and a hybrid meeting took place in Valencia, Spain in May 2023 to convene leading experts with emphasis on early career researchers (ECRs) across diverse scientific communities. Here we synthesize key insights and recommendations and offer these as a first effort to establish a consensus framework for advancing RNA virus discovery. First, we need interoperability through standardized methodologies, data-sharing protocols, metadata provision and interdisciplinary collaborations and offer specific examples as starting points. Second, as an emergent field, we recognize the need to incorporate cutting-edge technologies and knowledge early and often to improve omic-based viral detection and annotation as novel capabilities reveal new biology. Third, we underscore the significance of ECRs in fostering international partnerships to promote inclusivity and equity in virus discovery efforts. The proposed consensus framework serves as a roadmap for the scientific community to collectively contribute to the tremendous challenge of unveiling the RNA virosphere. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Meta-transcriptomics reveals potential virus transfer between Aedes communis mosquitoes and their parasitic water mites

Author

Ortiz-Baez, Ayda Susana, Holmes, Edward C., Charon, Justine, Pettersson, John, Hesson, Jenny C., Ortiz-Baez, Ayda Susana, Holmes, Edward C., Charon, Justine, Pettersson, John, and Hesson, Jenny C.

Abstract

Arthropods harbor a largely undocumented diversity of RNA viruses. Some arthropods, like mosquitoes, can transmit viruses to vertebrates but are themselves parasitized by other arthropod species, such as mites. Very little is known about the viruses of these ectoparasites and how they move through the host-parasite relationship. To address this, we determined the virome of both mosquitoes and the mites that feed on them. The mosquito Aedes communis is an abundant and widely distributed species in Sweden, in northern Europe. These dipterans are commonly parasitized by water mite larvae (Trombidiformes: Mideopsidae) that are hypothesized to impose negative selection pressures on the mosquito by reducing fitness. In turn, viruses are dual-host agents in the mosquito-mite interaction. We determined the RNA virus diversity of mite-free and mite-detached mosquitoes, as well as their parasitic mites, using meta-transcriptomic sequencing. Our results revealed an extensive RNA virus diversity in both mites and mosquitoes, including thirty-seven putative novel RNA viruses that cover a wide taxonomic range. Notably, a high proportion of viruses (20/37) were shared between mites and mosquitoes, while a limited number of viruses were present in a single host. Comparisons of virus composition and abundance suggest potential virus transfer between mosquitoes and mites during their symbiotic interaction. These findings shed light on virome diversity and ecology in the context of arthropod host-parasite-virus relationships.

Published
2022
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13. Human land use impacts viral diversity and abundance in a New Zealand river.

Author

French, Rebecca, Charon, Justine, Lay, Callum Le, Muller, Chris, and Holmes, Edward C

Subjects
LAND use, URBAN agriculture, AGRICULTURAL intensification, WATER birds, CITIES & towns, WATERSHEDS
Abstract

Although water-borne viruses have important implications for the health of humans and other animals, little is known about the impact of human land use on viral diversity and evolution in water systems such as rivers. We used metatranscriptomic sequencing to compare the diversity and abundance of viruses at sampling sites along a single river in New Zealand that differed in human land-use impacts, ranging from pristine to urban. From this, we identified 504 putative virus species, of which 97 per cent were novel. Many of the novel viruses were highly divergent and likely included a new subfamily within the Parvoviridae. We identified at least sixty-three virus species that may infect vertebrates—most likely fish and water birds—from the Astroviridae, Birnaviridae , Parvoviridae , and Picornaviridae. No putative human viruses were detected. Importantly, we observed differences in the composition of viral communities at sites impacted by human land use (farming and urban) compared to native forest sites (pristine). At the viral species level, the urban sites had higher diversity (327 virus species) than the farming (n  = 150) and pristine sites (n  = 119), and more viruses were shared between the urban and farming sites (n  = 76) than between the pristine and farming or urban sites (n  = 24). The two farming sites had a lower viral abundance across all host types, while the pristine sites had a higher abundance of viruses associated with animals, plants, and fungi. We also identified viruses linked to agriculture and human impact at the river sampling sites in farming and urban areas that were not present at the native forest sites. Although based on a small sample size, our study suggests that human land use can impact viral communities in rivers, such that further work is needed to reduce the impact of intensive farming and urbanisation on water systems. [ABSTRACT FROM AUTHOR]

Published
2022
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21. Dissecting the Biochemical and Transcriptomic Effects of a Locally Applied Heat Treatment on Developing Cabernet Sauvignon Grape Berries

Author

Lecourieux, Fatma, Kappel, Christian, Pieri, Philippe, Charon, Justine, Pillet, Jérémy, Hilbert, Ghislaine, Renaud, Christel, Gomès, Eric, Delrot, Serge, Lecourieux, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut des Sciences de la Vigne et du Vin (ISVV)-Université de Bordeaux (UB), Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)

Subjects
microclimat, Biodiversité et Ecologie, [SDV]Life Sciences [q-bio], metabolomics/metabolite profiling, Plant Science, berry development, climate change, grapevine, high temperature, microarrays, microclimate, baie de raisin, Biodiversity and Ecology, vitis vinifera, ddc:570, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Institut für Biochemie und Biologie, ComputingMilieux_MISCELLANEOUS, global change, Original Research, changement climatique, food and beverages, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, vigne, traitement thermique
Abstract

International audience; Reproductive development of grapevine and berry composition are both strongly influenced by temperature. To date, the molecular mechanisms involved in grapevine berries response to high temperatures are poorly understood. Unlike recent data that addressed the effects on berry development of elevated temperatures applied at the whole plant level, the present work particularly focuses on the fruit responses triggered by direct exposure to heat treatment (HT). In the context of climate change, this work focusing on temperature effect at the microclimate level is of particular interest as it can help to better understand the consequences of leaf removal (a common viticultural practice) on berry development. HT (+8 degrees C) was locally applied to clusters from Cabernet Sauvignon fruiting cuttings at three different developmental stages (middle green, veraison and middle ripening). Samples were collected 1, 7, and 14 days after treatment and used for metabolic and transcriptomic analyses. The results showed dramatic and specific biochemical and transcriptomic changes in heat exposed berries, depending on the developmental stage and the stress duration. When applied at the herbaceous stage, HT delayed the onset of veraison. Heating also strongly altered the berry concentration of amino acids and organic acids (e.g., phenylalanine, raminobutyric acid and malate) and decreased the anthocyanin content at maturity. These physiological alterations could be partly explained by the deep remodeling of transcriptome in heated berries. More than 7000 genes were deregulated in at least one of the nine experimental conditions. The most affected processes belong to the categories "stress responses," protein metabolism" and "secondary metabolism," highlighting the intrinsic capacity of grape berries to perceive HT and to build adaptive responses. Additionally, important changes in processes related to "transport," "hormone" and "cell wall" might contribute to the postponing of veraison. Finally, opposite effects depending on heating duration were observed for genes encoding enzymes of the general phenylpropanoid pathway, suggesting that the HI induced decrease in anthocyanin content may result from a combination of transcript abundance and product degradation.

Published
2017
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22. Contribution of Intrinsically Disordered Regions to the Interaction between the Potyviral Protein VPg and the Plant Translation Initiation Factor eIF4E

Author

Walter, Jocelyne, Barra, Amandine, Doublet, Bénédicte, Cerré, Nicolas, Charon, Justine, Roudet, Genevieve, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA), Centre hospitalier intercommunal du Pays de Cognac, ProdInra, Archive Ouverte, Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, and Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU)

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, VPg, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, virus phytopathogène, fast kinetic measurements, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, molten globule, potyvirus, phytopathogenic virus, eIF4E, santé des plantes, hydrodynamics, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, FRET, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, virologie végétale, protein intrinsic disorder, plant health, pathologie végétale, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract

Contribution of Intrinsically Disordered Regions to the Interaction between the Potyviral Protein VPg and the Plant Translation Initiation Factor eIF4E. 16. Rencontres de Virologie Végétale (RVV 2017)

Published
2017

23. Mutational robustness of intrinsically disordered proteins: a comparative study between the potyvirus intrinsically disordered VPg and the plant susceptibility factor eIF4E

Author

Walter, Jocelyne, Charon, Justine, Dialo, Mamadou, Hu, Yihua, Lachat, Joy, Leger, Thomas, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, ProdInra, Archive Ouverte, Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA.

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, VPg, virus phytopathogène, Yeast two hybrid, Potyvirus, error prone PCR, intrinsically disordered protein, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, phytopathogenic virus, eIF4E, santé des plantes, evolution, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, plant health, pathologie végétale, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
Abstract

UMR 1332 BFP - Equipe Virologie; Mutational robustness of intrinsically disordered proteins: a comparative study between the potyvirus intrinsically disordered VPg and the plant susceptibility factor eIF4E. 16. Rencontres de Virologie Végétale (RVV 2017)

Published
2017

24. Assessment of the viral protein VPg intrinsic disorder contribution to the Potato virus Y adaptation to eIF4E-mediated resistance of pepper (Capsicum annuum)

Author

Charon, Justine, Barra, Amandine, Walter, Jocelyne, Simon, Vincent, Moury, Benoit, MILLOT, Pauline, Hébrard, Eugénie, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Université Bordeaux Segalen - Bordeaux 2, Station de Pathologie Végétale (AVI-PATHO), Institut National de la Recherche Agronomique (INRA), UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Unité de Pathologie Végétale (PV), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD). FRA., Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, adaptation, PVY, capsicum annuum, piment, potyvirus, phytopathogenic virus, potato virus y, santé des plantes, evolution, pathologie végétale, virus à arn, VPg, résistance variétale, virus phytopathogène, eif4e, culture legumiere, intrinsically disordered protein, virus y de la pomme de terre, rna virus, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, chilli pepper, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, plant health, mutation virale
Abstract

UMR BFP - Equipe Virologie UMR BFP - Equipe VirologieUMR BFP - Equipe Virologie; National audience

Published
2017

25. VvGOLS1 and VvHsfA2 are Involved in the Heat Stress Responses in Grapevine Berries

Author

Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, Lecourieux, David, Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, and Lecourieux, David

Abstract

Among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. Grapevine is the most cultivated fruit plant throughout the world, and grapes are used for wine production and human consumption. The molecular mechanisms involved in grapevine tolerance to high temperature, especially at the fruit level, are poorly understood. To better characterize the sensitivity of berries to the microenvironment, high temperature conditions were locally applied to Vitis vinifera Cabernet Sauvignon clusters. Two genes, VvGOLS1 and VvHsfA2, up-regulated by this treatment, were identified and further characterized. The expression profile of VvGOLS1 correlated positively with galactinol accumulation in heat-stressed berries. However, no galactinol derivatives, such as raffinose and stachyose, accumulated upon heat stress. Heterologous expression of VvGOLS1 in Escherichia coli showed that it encodes a functional galactinol synthase. Transient expression assays showed that the heat stress factor VvHsfA2 transactivates the promoter of VvGOLS1 in a heat stress-dependent manner. Taken together, our results highlight the intrinsic capacity of grape berries to perceive heat stress and to initiate adaptive responses, suggesting that galactinol may play a signaling role in these responses

Published
2017

26. Direct impact of high temperatures on grapevine berry development: a merge transcriptomic, proteomic and metabolomic survey

Author

LECOURIEUX, Fatma, Kappel, C., Pieri, Philippe, Charon, Justine, Pillet, Jérémy, Claverol, S., Lunn, J., Hilbert, Ghislaine, Renaud, Christel, Bonneu, Marc, GOMES, Eric, Delrot, Serge, Lecourieux, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), University of Potsdam, Université de Bordeaux Ségalen [Bordeaux 2], Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, and Institut National de Recherche Agronomique (INRA). UMR Ecophysiologie et Génomique Fonctionnelle de la Vigne (1287).

Subjects
[SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

28. Structural basis of the interaction between potyviral VPg and the plant resistance factor eIF4E : evidences for an a-helix induced folding upon association

Author

Walter, Jocelyne, Barra, Amandine, Charon, Justine, Doublet, Bénédicte, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

29. Characterization of intrinsic disorder involvement in potyvirus adaptation using PVY/Pepper pathosystem as model

Author

Charon, Justine, Walter, Jocelyne, Barra, Amandine, Moury, Benoît, Hébrard, Eugénie, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), and ProdInra, Migration

Subjects
Adaptability, Intrinsic disorder, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], [SDV.OT] Life Sciences [q-bio]/Other [q-bio.OT], potyvirus, mutational robustness, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

30. Contribution du désordre intrinsèque des protéines aux fonctions impliquées dans le cycle viral et l'évolution adaptative des virus à ARN : étude appliquée au genre modèle Potyvirus

Author

Charon, Justine, STAR, ABES, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université de Bordeaux, Thierry Michon, Michon, Thierry, Forterre, Patrick, Blanchard, Alain, Terradot, Laurent, Blanc, Stéphane, Alain Blanchard [Président], Laurent Terradot [Rapporteur], Patrick Forterre, and Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)

Subjects
RNA viruses, Protein intrinsic disorder, Potyvirus, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Virus à ARN, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, Adaptation, Désordre intrinsèque des protéines, Potato virus Y
Abstract

Proteins are essential actors involved in a majority of molecular and cellular processes. The features associated with the functions of these macromolecules have been recently questioned with the emergence of the intrinsic disorder concept. It defines the transitory or permanent lack of 3D structure in some proteins or regions as directly related to their functions. Among RNA viruses, the properties of disordered proteins may be linked to the ability of these microorganisms to hijack the host machinery by interacting with multiple partners, as well as to adapt to the multiple constraints they must face as obligatory parasites. This work focuses on the Potyvirus genus, which includes some of the most damaging plant pathogens studied to date. The goal of this thesis was to explore the functions associated with intrinsic disorder in the infectious cycle of this viral genus as well as in its process of adaptation. Our studies have shown that i) intrinsic disorder is ubiquitous in potyviruses; ii) intrinsically disordered regions (IDR) of some of potyviral proteins are likely to be associated with important functions for the viral cycle ; iii) IDR are generally less evolutionary constrained, suggesting an adaptive potential of these regions ; iv) predicted IDR seem to favor the appearance of mutations and therefore virus ability to accumulate genetic diversity during its evolution in natural host ; v) an experimental disorder modulation within the Viral genome-linked (VPg) protein has been demonstrated as positively correlated with the adaptive ability of the Potato virus Y to overcome the pvr23 recessive resistance in pepper., Les protéines sont des acteurs majeurs dans les processus moléculaires et cellulaires d’un organisme. La remise en question des modalités associées aux fonctions de ces macromolécules a récemment été apportée par le concept de désordre intrinsèque. Celui-ci définit l’absence (transitoire ou permanente) de structure tridimensionnelle de certaines protéines ou régions protéiques comme étant directement liée à leurs fonctions. Chez les virus à ARN, les propriétés des protéines ou régions désordonnées semblent associées aux capacités de ces micro-organismes à détourner la machinerie cellulaire de l’hôte en interagissant avec de multiples partenaires, et à s’adapter aux nombreuses contraintes auxquelles ils doivent faire face en tant que parasites obligatoires. Ce travail porte sur les potyvirus, figurant parmi les pathogènes de plantes les plus dommageables étudiés à ce jour. L'objectif de cette thèse a été d’explorer les fonctions associées au désordre intrinsèque dans le cycle infectieux des potyvirus ainsi que dans le processus d’adaptation. Notre approche a ainsi démontré que : i) le désordre est ubiquitaire chez le genre Potyvirus ; ii) les régions de désordre conservées chez plusieurs protéines de potyvirus semblent être associées à leur(s) fonction(s) pendant l'infection ; iii) les régions désordonnées sont généralement associées à moins de contraintes évolutives, suggérant ainsi leur implication dans les processus adaptatifs des potyvirus ; iv) les régions prédites comme désordonnées semblent privilégier l’apparition de mutations et donc la capacité d’un virus à accumuler de la diversité génétique au cours de l'évolution sur son hôte naturel ; v) ce travail a permis de corréler le taux en désordre de la protéine viral genome-linked (VPg) du Potato virus Y à sa capacité à s’adapter à la résistance récessive pvr23 du piment.

Published
2015

32. Experimental data on the interactions between a viral pre-molten globule (VPg) and an eukaryotic translation initiation factor (eIF4E)

Author

Michon, Thierry, Barra, Amandine, Charon, Justine, Hébrard, Eugénie, Longhi, Sonia, Moury, Benoît, Walter, Jocelyne, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Centre National de la Recherche Scientifique (CNRS), Unité de Pathologie Végétale (PV), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

33. An attempt to experimentally assess the contribution of intrinsic disorder to the virus adaptation

Author

Charon, Justine, Barra, Amandine, Hébrard, Eugénie, Longhi, Sonia, Moury, Benoît, Walter, Jocelyne, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Centre National de la Recherche Scientifique (CNRS), Unité de Pathologie Végétale (PV), and Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

34. Direct impact of high temperature on the grapevine berry proteome

Author

LECOURIEUX, Fatma, KAPPEL, Christian, PIERI, Philippe, CHARON, Justine, GOMES, Eric, DELROT, Serge, LECOURIEUX, David, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2013

36. Direct impact of high temperature on Cabernet Sauvignon berries development and metabolism

Author

Lecourieux, David, Pillet, Jérémy, Lecourieux, Fatma, Kappel, Christian, Pieri, Philippe, Charon, Justine, Hilbert, Ghislaine, Renaud, Christel, Gomes, Eric, Delrot, Serge, Ecophysiologie et Génomique Fonctionnelle de la Vigne (UMR EGFV), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Université Victor Segalen - Bordeaux 2-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDE] Environmental Sciences, [SDV]Life Sciences [q-bio], [SDE]Environmental Sciences, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2013

37. Contribution du désordre intrinsèque des protéines aux fonctions impliquées dans le cycle viral et l'évolution adaptative des virus à ARN. Etude appliquée au genre Potyvirus

Author

Charon, Justine, Thierry Michon, ProdInra, Migration, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), and Université de Bordeaux

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BV] Life Sciences [q-bio]/Vegetal Biology, these, [SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy

38. VvGOLS1 and VvHsfA2 are Involved in the Heat Stress Responses in Grapevine Berries

Author

Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, Lecourieux, David, Pillet, Jérémy, Egert, Aurélie, Pieri, Philippe, Lecourieux, Fatma, Kappel, Christian, Charon, Justine, Gomès, Eric, Keller, Felix, Delrot, Serge, and Lecourieux, David

Abstract

Among various environmental factors, temperature is a major regulator affecting plant growth, development and fruit composition. Grapevine is the most cultivated fruit plant throughout the world, and grapes are used for wine production and human consumption. The molecular mechanisms involved in grapevine tolerance to high temperature, especially at the fruit level, are poorly understood. To better characterize the sensitivity of berries to the microenvironment, high temperature conditions were locally applied to Vitis vinifera Cabernet Sauvignon clusters. Two genes, VvGOLS1 and VvHsfA2, up-regulated by this treatment, were identified and further characterized. The expression profile of VvGOLS1 correlated positively with galactinol accumulation in heat-stressed berries. However, no galactinol derivatives, such as raffinose and stachyose, accumulated upon heat stress. Heterologous expression of VvGOLS1 in Escherichia coli showed that it encodes a functional galactinol synthase. Transient expression assays showed that the heat stress factor VvHsfA2 transactivates the promoter of VvGOLS1 in a heat stress-dependent manner. Taken together, our results highlight the intrinsic capacity of grape berries to perceive heat stress and to initiate adaptive responses, suggesting that galactinol may play a signaling role in these responses

39. First experimental assessment of protein intrinsic disorder involvement in an RNA virus natural adaptive process

Author

Eugénie Hébrard, Amandine Barra, Benoît Moury, Justine Charon, Jocelyne Walter, Pauline Millot, Thierry Michon, Charon, Justine, Moury, Benoit, Michon, Thierry, Biologie du fruit et pathologie (BFP), Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Génomique, développement et pouvoir pathogène (GD2P), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ministère Français de l’enseignement supérieur et de la Recherche (JC Fellowship), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, and Station de Pathologie Végétale (AVI-PATHO)

Subjects
viral protein genome-linked, 0301 basic medicine, eukaryotic translation initiation factor 4E, Proteome, Acclimatization, [SDV]Life Sciences [q-bio], Virologie, Pilot Projects, Viral Nonstructural Proteins, medicine.disease_cause, Pathosystem, potyvirus, pathologie végétale, ComputingMilieux_MISCELLANEOUS, Genetics, Mutation, Vegetal Biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Protein Stability, resistance breakdown, Potyvirus, protein intrinsic disorder, RNA virus adaptation, Adaptation, Physiological, Biological Evolution, virus y de la pomme de terre, Agricultural sciences, fitness, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], protéine virale, Ribonucleoproteins, Potato virus Y, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Capsicum, Phytopathology and phytopharmacy, Genome, Viral, Biology, Virus, capsicum annuum, Evolution, Molecular, resistance, 03 medical and health sciences, désordre intrinsèque des protéines, Virology, capacité d'adaptation, medicine, Molecular Biology, Gene, Discoveries, Ecology, Evolution, Behavior and Systematics, virus phytopathogène, breakdown, 030102 biochemistry & molecular biology, eukaryotic translation, RNA, RNA virus, biology.organism_classification, Phytopathologie et phytopharmacie, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, 030104 developmental biology, initiation factor 4E, évolution génétique des populations, mutation, Biologie végétale, Sciences agricoles
Abstract

Intrinsic disorder (ID) in proteins is defined as a lack of stable structure in physiological conditions. Intrinsically disordered regions (IDRs) are highly abundant in some RNA virus proteomes. Low topological constraints exerted on IDRs are expected to buffer the effect of numerous deleterious mutations and could be related to the remarkable adaptive potential of RNA viruses to overcome resistance of their host. To experimentally test this hypothesis in a natural pathosystem, a set of four variants of Potato virus Y (PVY; Potyvirus genus) containing various ID degrees in the Viral genome-linked (VPg) protein, a key determinant of potyvirus adaptation, was designed. To estimate the ID contribution to the VPg-based PVY adaptation, the adaptive ability of the four PVY variants was monitored in the pepper host (Capsicum annuum) carrying a recessive resistance gene. Intriguingly, the two mutants with the highest ID content displayed a significantly higher ability to restore infection in the resistant host, whereas the less intrinsically disordered mutant was unable to restore infection. The role of ID on virus adaptation may be due either to a larger exploration of evolutionary pathways or the minimization of fitness penalty caused by resistance-breaking mutations. This pioneering study strongly suggests the positive impact of ID in an RNA virus adaptive capacity.

Published
2018

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