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Today’s data-driven world requires earth and environmental scientists to have skills at the intersection of domain and data science. These skills are imperative to harness information contained in a growing volume of complex data to solve the world’s most pressing environmental challenges. Despite the importance of these skills, Earth and Environmental Data Science (EDS) training is not equally accessible, contributing to a lack of diversity in the field. This creates a critical need for EDS training opportunities designed specifically for underrepresented groups. In response, we developed the Earth Data Science Corps (EDSC) which couples a paid internship for undergraduate students with faculty training to build capacity to teach and learn EDS using Python at smaller Minority Serving Institutions. EDSC faculty participants are further empowered to teach these skills at their home institutions which scales the program beyond the training lead by our team. Using a Rasch modeling approach, we found that participating in the EDSC program had a significant impact on undergraduate learners’ comfort and confidence with technical and nontechnical data science skills, as well as their science identity and sense of belonging in science, two critical aspects of recruiting and retaining members of underrepresented groups in STEM. Supplementary materials for this article are available online.

Abstract The red snapper (Lutjanus campechanus) has one of the most valued fisheries in the southeastern United States and has been largely studied for its aquaculture potential with varied results that revealed production challenges. This study focused on addressing challenges in larval rearing and juvenile production of this species. Broodstock fish were acclimated in a 60 m3 tank equipped with recirculating aquaculture system (RAS) and temperature control. After 1 year in captivity, fish volitionally spawned and larval rearing trials were conducted in replicated flow‐through seawater tanks ranging from 0.4 to 2.4 m3, at a temperature range of 24–26°C. Enriched s‐strain rotifers, Brachionus rotundiformis (lorica length of 100–210 μm), were used for first feeding. Enriched Artemia sp. were gradually introduced after 18 days post hatch (DPH). The survival rate before metamorphosis (12 DPH) averaged 66.09 ± 0.08%. Survival rate from yolk‐sac larvae to post‐metamorphic early juvenile averaged 4.43 ± 0.01%. At 40 DPH, early red snapper juveniles averaged 0.35 ± 0.02 g in weight and 28.83 ± 0.60 mm in standard length. A total of 31,849 fully weaned juveniles were harvested. This study demonstrates that small strain rotifers can be used exclusively as first feeding prey organism for red snapper larvae, representing a significant advancement for red snapper juvenile production.

Abstract Introduction Gonorrhea, caused by Neisseria gonorrhoeae (NG), is the second most common bacterial sexually transmitted infection (STI). Rates of antimicrobial resistance to standard care are increasing worldwide, with many antibiotic classes now ineffective against NG. Gepotidacin is a first-in-class, bactericidal, triazaacenaphthylene antibiotic that inhibits bacterial DNA replication by inhibition of two enzymes, where a single target-specific mutation does not significantly impact susceptibility. Gepotidacin confers activity against NG, including most strains resistant to marketed antibiotics. Here, we describe the design of a phase 3 clinical trial (EAGLE-1; NCT04010539) evaluating gepotidacin for the treatment of uncomplicated urogenital gonorrhea. Methods This phase 3, randomized, multicenter, sponsor-blinded, noninferiority study across six countries is comparing the efficacy of gepotidacin with ceftriaxone plus azithromycin in 400 patients with uncomplicated urogenital gonorrhea (microbiological intent-to-treat population) and assessing the safety of gepotidacin in approximately 600 patients (intent-to-treat population). Eligible participants 12 years of age or older with clinical suspicion of urogenital gonococcal infection and a NG-positive urogenital sample and/or purulent discharge are randomized 1:1 to receive oral gepotidacin (2 × 3000 mg 10–12 h apart) or ceftriaxone (500 mg, intramuscular) plus azithromycin (1 g, oral). The primary endpoint is culture-confirmed bacterial eradication of NG from the urogenital site at the test-of-cure (days 4–8) visit. Planned Outcomes This trial was designed in accordance with US Food and Drug Administration (2015) and European Medicines Agency (2011) guidance, particularly the primary endpoint and microbiological evaluability requirements. This study will help characterize the risk–benefit profile of gepotidacin for treating uncomplicated urogenital gonorrhea. Gepotidacin is an important potential treatment for gonorrhea to help address the urgent unmet need of multidrug resistance and the increasingly limited number of oral treatment options. Trial Registration ClinicalTrials.gov identifier, NCT04010539.

Summary: Background: Although immunomodulators have established benefit against the new coronavirus disease (COVID-19) in general, it is uncertain whether such agents improve outcomes without increasing the risk of secondary infections in the specific subgroup of previously immunocompromised patients. We assessed the effect of immunomodulators on outcomes of immunocompromised patients hospitalized for COVID-19. Methods: The protocol was prospectively registered with PROSPERO (CRD42022335397). MEDLINE, Cochrane Central Register of Controlled Trials and references of relevant articles were searched up to 01-06-2022. Authors of potentially eligible randomized controlled trials were contacted to provide data on immunocompromised patients randomized to immunomodulators vs control (i.e., placebo or standard-of-care). Findings: Eleven randomized controlled trials involving 397 immunocompromised patients hospitalized for COVID-19 were included. Ten trials had low risk of bias. There was no difference between immunocompromised patients randomized to immunomodulators vs control regarding mortality [30/182 (16.5%) vs 41/215 (19.1%); RR 0.93, 95% CI 0.61–1.41; p = 0.74], secondary infections (RR 1.00, 95% CI 0.64–1.58; p = 0.99) and change in World Health Organization ordinal scale from baseline to day 15 (weighed mean difference 0.27, 95% CI -0.09–0.63; p = 0.15). In subgroup analyses including only patients with hematologic malignancy, only trials with low risk of bias, only trials administering IL-6 inhibitors, or only trials administering immunosuppressants, there was no difference between comparators regarding mortality. Interpretation: Immunomodulators, compared to control, were not associated with harmful or beneficial outcomes, including mortality, secondary infections, and change in ordinal scale, when administered to immunocompromised patients hospitalized for COVID-19. Funding: Hellenic Foundation for Research and Innovation.

IntroductionAntimalarial drugs including artemisinin-based combination therapy (ACT) regimens and sulphadoxine-pyrimethamine (SP) are used in Ghana for malaria therapeutics and prophylaxis respectively. The genetic basis of Plasmodium falciparum development of drug resistance involves single nucleotide polymorphisms in genes encoding proteins for multiple cellular and metabolic processes. The prevalence of single nucleotide polymorphisms in nine P. falciparum genes linked to ACT and SP resistance in the malaria parasite population was determined.MethodsArchived filter paper blood blot samples from patients aged 9 years and below with uncomplicated malaria reporting at 10 sentinel sites located in three ecological zones for the Malaria Therapeutic Efficacy Studies were used. The samples used were collected from 2007-2018 malaria transmission seasons and mutations in the genes were detected using PCR and Sanger sequencing.ResultsIn all 1,142 samples were used for the study. For falcipain-2 gene (pffp2), Sanger sequencing was successful for 872 samples and were further analysed. The prevalence of the mutants was 45% (392/872) with pffp2 markers V51I and S59F occurring in 15.0% (128/872) and 3.0% (26/872) of the samples respectively. Prevalence of other P. falciparum gene mutations: coronin (pfcoronin) was 44.8% (37/90); cysteine desulfurase (pfnfs) was 73.9% (68/92); apicoplast ribosomal protein S10 (pfarps10) was 36.8% (35/95); ferredoxin (pffd) was 8.8% (8/91); multidrug resistance protein-1 (pfmrp1) was 95.2.0% (80/84); multidrug resistance protein-2 (pfmrp2) was 91.4% (32/35); dihydrofolate reductase (pfdhfr) was 99.0% (84/85); dihydropteroate synthase (pfdhps) was 72% (68/95).DiscussionThe observation of numerous mutations in these genes of interest in the Ghanaian isolates, some of which have been implicated in delayed parasite clearance is of great interest. The presence of these genotypes may account for the decline in the efficacies of ACT regimens being used to treat uncomplicated malaria in the country. The need for continuous monitoring of these genetic markers to give first-hand information on parasite susceptibility to antimalarial drugs to inform policy makers and stakeholders in malaria elimination in the country is further discussed.

Photometric simulations using both daylight and electric lighting were performed to compare the energy use of conventional high-pressure sodium (HPS) greenhouse lighting to that of light-emitting diode (LED) lighting. Photometric simulations of a hypothetical greenhouse were performed in three different geographic locations in the United States with widely different annual daylight availability: Albany, NY, Fairbanks, AK, and Phoenix, AZ. Simulation conditions included summer and winter, overcast and clear skies, and several lighting layouts and distributions. The analysis showed that, while maintaining the criteria levels of photosynthetic photon flux density, lighting energy savings were primarily attributable to increased LED source efficacy rather than HPS. Secondary energy savings were attributable to the ability to continuously dim LED lighting in response to daily and seasonal changes in daylight. Despite options for LED luminaires with a slim form factor, reduced crop shading compared with larger conventional HPS luminaires did not result in significant lighting energy savings.

Mucoepidermoid carcinoma (MEC) is an uncommon type of salivary gland tumor that can present as an endobronchial neoplasm, most commonly in the adult population. Neuroendocrine carcinoid tumors comprise the majority of bronchial neoplasms in the pediatric population and are nearly indistinguishable from MEC on imaging. We present a rare case of MEC in a 3-year-old presenting with recurrent symptoms of lower airway obstruction and discuss its typical associated symptoms and imaging features.

Waste generation is one of the most relevant environmental aspects of the construction industry. About 47 million tons of construction and demolition waste are collected annually by Brazilian municipalities. One of the activities that generates waste is cutting chases on walls for installations. However, there are no waste generation indicators for this activity. Understanding waste generation processes enables managers to prevent them and promote their proper environmental management. This study assessed the generation of waste resulting from the cutting of clay bricks for electrical installations using three tools: milling cutter, marble saw, and cold chisel. The study included data collected from residential construction works and experimental data collected from the construction of real-scale walls. In a laboratory, five different wall configurations were built and the three tools mentioned were used to cut a chase on the walls. The results were statistically analyzed to define a waste generation index (WGI) by linear regression. The type of tool employed had no influence on the waste generation index, which was 26.5 ± 2.6 kg/m2. However, the tools used directly influenced the quality of the service, productivity, and the volume of waste generated. The waste from the milling cutter showed the smallest maximum aggregate size and the largest bulk density, followed by the waste resulting from the marble saw and the cold chisel. The marble saw and cold chisel waste samples had around 78% of their composition in the coarse aggregate grain size range. The milling cutter waste samples were the finest and had on average 60% of their composition in the fine aggregate grain size range. The width of the chases made with the milling cutter were smaller and more consistent than those made with the cold chisel, which showed irregularities and larger dimensions than necessary. From the waste generation indicators obtained in this study, construction managers will be able to choose more appropriate cutting tools and improve their planning and management systems to minimize associated environmental impacts.

Sulfated glycosaminoglycans (sGAG) show interaction with biological mediator proteins. Although collagen-based biomaterials are widely used in clinics, their combination with high-sulfated hyaluronan (sHA3) is unexplored. This study aims to functionalize a collagen-based scaffold (Mucograft®) with sHA3 via electrostatic (sHA3/PBS) or covalent binding to collagen fibrils (sHA3+EDC/NHS). Crosslinking without sHA3 was used as a control (EDC/NHS Ctrl). The properties of the sHA3-functionalized materials were characterized. In vitro growth factor and cytokine release after culturing with liquid platelet-rich fibrin was performed by means of ELISA. The cellular reaction to the biomaterials was analyzed in a subcutaneous rat model. The study revealed that covalent linking of sHA3 to collagen allowed only a marginal release of sHA3 over 28 days in contrast to electrostatically bound sHA3. sHA3+EDC/NHS scaffolds showed reduced vascular endothelial growth factor (VEGF), transforming growth factor beta 1 (TGF-β1) and enhanced interleukin-8 (IL-8) and epithelial growth factor (EGF) release in vitro compared to the other scaffolds. Both sHA3/PBS and EDC/NHS Ctrl scaffolds showed a high proinflammatory reaction (M1: CD-68+/CCR7+) and induced multinucleated giant cell (MNGC) formation in vivo. Only sHA3+EDC/NHS scaffolds reduced the proinflammatory macrophage M1 response and did not induce MNGC formation during the 30 days. SHA3+EDC/NHS scaffolds had a stable structure in vivo and showed sufficient integration into the implantation region after 30 days, whereas EDC/NHS Ctrl scaffolds underwent marked disintegration and lost their initial structure. In summary, functionalized collagen (sHA3+EDC/NHS) modulates the inflammatory response and is a promising biomaterial as a stable scaffold for full-thickness skin regeneration in the future.

Introduction/Main Objectives: Leadership research tries to find strategies and ways of good leaders in managing organizations throughout history. With the many studies conducted, various theories of leadership were born. Spiritual Leadership is a synthesis of previous leadership, namely ethics, authenticity, stewardship and other theories. SL seeks to fully pay attention to the dimensions in humans so that individuals experience fullness in the organization. Research reveals that SL has fostered intrinsic value in individuals, resolved problems in organizations well and promoted a sustainable workplace. Background Problems: With a variety of studies related to SL, we try to map the number of journals each year, then see what keywords are correlated with SL, and find influential SL authors. Novelty: This study will look at the latest research themes between 2017-2021 and be assisted by Bibliometric analysis. Research Methods: The search was carried out in the Scopus database, on November 8, 2021. In the search terms in all titles, abstracts, and keywords, the word “spiritual leadership” was written. The filter used is the document year range from 2017 to 2021. Then the results of the scopus analysis are mapped using VosViewer. Finding/Results: The results show an average of 36 articles publish each year. The three countries that produce the most writing are America, China, and Indonesia. Authors like Fry, Usman, Ali, and Yang most frequently cited by other authors. Network analysis and density visualization identify keywords related to SL: workplace spirituality, work engagement, spiritual well-being, organizational commitment, employee performance, job satisfaction and organizational citizenship behaviour. Conclusion: SL research can be developed more broadly on the scope of themes that appear little, such as vision, job satisfaction, employee performance, organizational citizenship behavior, and work engagement. Second, SL research whose scope does not yet exist, such as Education, organizational culture and religion.

In this study, Lippia origanoides Kunth, native to the Alto Patía region in Southwest Colombia, and Pichia guilliermondii LV196, an inactivated yeast from the germplasm bank of Agrosavia (Colombian Agricultural Research Corporation), alone or combined, were tested for their long-term effect on rumen fermentation and methanogenesis whilst also characterising their effect on bacterial and methanogen communities. Whereas essential oils act through selective inhibition of microbial groups, yeasts are thought to work through the selective stimulation of key microbes in the rumen. We hypothesized that yeast supplementation could modulate the antimicrobial effect of a high thymol-containing oregano oil, allowing a more efficient feed utilization whilst decreasing methane production. When added to a rumen simulating fermentor (RUSITEC), L. origanoides Kunth at 132 µL/d had a detrimental effect on rumen fermentation which was accompanied by a reduction in the relative abundance of protozoa and fungi and a profound impact on the bacterial and archaeal communities. P. guilliermondii LV196 at 0.5 g/L, however, had no effect on fermentation parameters or nutrient utilization, and neither changes in microbial abundances or in the structure of bacterial and archaeal communities were observed. P. guilliermondii LV196 did not stimulate microbial numbers nor activity and, consequently we could not test whether it could have counterbalanced the antimicrobial effect of the essential oil. Future studies need to both investigate lower levels of essential oil addition, but also to re-examine the effects of P. guillermondii in the rumen and/or to replace it with other yeast of known biological activity when combined with oils extracted from L. origanoides Kunth.

Abstract Background The knowledge about the experience of informal caregivers who provide care to people with moderate to advanced dementia in a domestic home setting is limited. A consequence of long hours of caregiving in addition to dealing with normal challenges of daily living is their experience of a poor quality of life. Some of their experiences may be described in terms of a feeling of powerlessness to make changes during care provision. This feeling may also suggest an experience of moral distress. The aim of this systematic review is to synthesise qualitative evidence relating to these experiences. Methods This review adopts a narrative synthesis approach. A search will be conducted for studies written in the English language in the bibliographic databases MEDLINE Complete, CINAHL, EMBASE, PsycINFO, Web of Science and Academic Search Complete covering periods from 1984 to present. Included studies will be qualitative or mixed-methods designs. The search terms will be related to dementia and caregivers, and the process will be focused on dementia at the moderate to the advanced stages within the domestic home setting. Reference lists of included papers will also be searched for additional relevant citations. Search terms and strategies will be checked by two independent reviewers. The identification of abstracts and full texts of studies will be done by the author, while the quality and the risk of bias will also be checked by the two independent reviewers. Discussion Psychological distress is cited as an experience reported within informal caregiving. For the caregiver, it is associated with a negative impact on general health. To date, no synthesis exists on the specific experience of informal caregiving for people with moderate to advanced dementia within the domestic home setting. This review considers that variation of accounts contributes to how the informal caregivers’ general experience is explored in future research. This may enable gaps in current knowledge to be highlighted within the wider context of caregiving in the domestic home setting. Systematic review registration This review is registered with PROSPERO ( CRD42020183649 ).

Abstract PIM and PI3K/mTOR pathways are often dysregulated in prostate cancer, and may lead to decreased survival, increased metastasis and invasion. The pathways are heavily interconnected and act on a variety of common effectors that can lead to the development of resistance to drug inhibitors. Most current treatments exhibit issues with toxicity and resistance. We investigated the novel multikinase PIM/PI3K/mTOR inhibitor, AUM302, versus a combination of the PIM inhibitor, AZD-1208, and the PI3K/mTOR inhibitor BEZ235 (Dactolisib) to determine their impact on mRNA and phosphoprotein expression, as well as their functional efficacy. We have determined that around 20% of prostate cancer patients overexpress the direct targets of these drugs, and this cohort are more likely to have a high Gleason grade tumour (≥ Gleason 8). A co-targeted inhibition approach offered broader inhibition of genes and phosphoproteins in the PI3K/mTOR pathway, when compared to single kinase inhibition. The preclinical inhibitor AUM302, used at a lower dose, elicited a comparable or superior functional outcome compared with combined AZD-1208 + BEZ235, which have been investigated in clinical trials, and could help to reduce treatment toxicity in future trials. We believe that a co-targeting approach is a viable therapeutic strategy that should be developed further in pre-clinical studies.

Celiac disease (CD) is a multisystem disorder known to manifest in a multitude of ways to include diarrhea, anemia, and nutritional deficiencies. The malabsorptive state can present as certain classical conditions such as autoimmune gastritis and osteopenia/osteoporosis but scurvy is less recognized within the literature. In this case, we present a unique presentation of scurvy as a result of an undiagnosed CD.

Understanding coastal circulation and how it may alter in the future is important in island settings, especially in the South West Pacific, where communities rely heavily upon marine resources, and where sea level rise (SLR) is higher than the global average. In this study we explore the use of an unstructured-mesh finite-volume modelling approach to assist in filling the knowledge gaps with respect to coastal circulation in remote island locations—selecting the Vanuatu and New Caledonia archipelagos as our example study site. Past limited observations and modelling studies are leveraged to construct and verify a regional/coastal ocean model based on the Finite-Volume Community Ocean Model (FVCOM). Following verification with respect to tidal behaviour, we investigate how changes in wind speed and direction, and SLR, alter coastal water levels and coastal currents. Results showed tidal residual circulation was typically associated with flow separation at headlands and islands. Trade winds had negligible effect on water levels at the coast, however, wind-residual circulation was sensitive to both wind speed and direction. Wind-residual currents were typically strongest close to coastlines. Wind residual circulation patterns were strongly influenced by Ekman flow, while island blocking, topographic steering and geostrophic currents also appear to influence current patterns. Tidal amplitudes and phases were unchanged due to SLR of up to 2 m, while maximum current speeds altered by as much as 20 cm/s within some coastal embayments. Non-linear relationships between SLR and maximum current speeds were seen at some coastal reef platform sites. Under higher sea levels, tidal residual currents altered by less than ±2 cm/s which is relatively significant given maximum tidal residual current speeds are typically below 10 cm/s. Our findings indicate that under higher sea levels, coastal processes governing sediment transport, pollutant dispersal and larval transport are likely to alter, which may have implications for coastal environments and ecosystems. Given winds influence coastal circulation and subsequent coastal processes, changes in trade winds due to climate change may act to further alter coastal processes. It is felt that the current modelling approach can be applied to other regions to help fill critical knowledge gaps.