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4. Description Et Potentiel Antagoniste D’une Nouvelle Souche D’actinobactérie Isolée De La Cote De Tipaza

Author

Mathieu Florence Mathieu Florence, Chaouch Fawzia Chaabane Chaouch Fawzia Chaabane, Mellouk Imène Mellouk Imène, Mokrane Salim Mokrane Salim, Meklat Atika, Sabaou Nasserdine, Bouras Noureddine Bouras Noureddine, Ait Yahia Ahmed Ait Yahia Ahmed, and Nalubega Fatuma Nalubega Fatuma

Abstract

Une nouvelle souche, designee TA4, a ete isolee d’un echantillon de l’eau de mer preleve de la cote de Tipaza (36°35’ Nord, 2°26’ Est)par la methode de suspension dilution, apres un etalement sur le milieu chitine-vitamines agar additionned’unagent antifongique(actidionea 50mg/l). L’etude taxonomique basee sur les criteres morphologique, physiologique et moleculaire arapproche cette souche des especesde Streptomycesolivaceus et S. pactum . L’etude de l’activiteantimicrobienne sur milieu ISP2 soliderevele que lasoucheTA4 possede une forte activite contre deux bacteries a Gram positif ( Staphylococcus aureus 639c resistante a la meticillineet Bacillus subtilis ATCC 6633 ) . La cinetique de la production de l’activite antibacterienne par la methode de diffusion par des puits suivie pendant 10 jours sur le milieu ISP2 liquide a montre que l’incubation pendant 5 jours donne une meilleure activite. Ce resultat a ete confirme par une deuxieme cinetique par la methode de diffusion de disque de papiers imbibes par les extraits a l’acetate d’ethyle et au dichloromethane. La deuxieme cinetique montre aussi quela plus grande activite antibacterienne a ete obtenue avec l’extrait al’acetate d’ethyle. L’analyse par HPLC de cet extrait montre la presence de deux composes hydrophobes et le spectre UV-visible montre qu’ils sont de nature aromatique ou heterocyclique. Mot cles: Biotope marin, Streptomyces , taxonomie, activite antibacterienne.

Published
2016

8. PLANOMONOSPORA, SACCHAROTHRIX AND ACTINOPHYTOCOLA GENERA IN SAHARAN SOILS OF ALGERIA: ISOLATION, TAXONOMIC IDENTIFICATION AND ANTAGONISTIC PROPERTIES.

Author

Chaouch, Fawzia Chaabane, Bouznada, Khaoula, Bouras, Noureddine, Meklat, Atika, Tata, Samira, Mokrane, Salim, Lamari, Lynda, Florence, Mathieu, Spröer, Cathrin, Klenk, Hans-Peter, and Sabaou, Nasserdine

Subjects
*ACTINOBACTERIA, *ANTI-infective agents, *SOIL microbiology, *DESERT soils, *THERAPEUTIC use of bacteria, *SEQUENCE analysis, *PHYSIOLOGY
Abstract

With the aim of studying the biodiversity and the antimicrobial potential of three rare actinobacterial genera: Planomonospora, Saccharothrix and Actinophytocola in Algerian Saharan soils, 65 isolates representing the morphological characteristics of Planomonospora (17) and Saccharothrix/Actinophytocola (48) were isolated from 13 soil samples at 4 different sites in southern Algeria. The isolation was carried out on humic acid-vitamin agar medium using dilution techniques with several soil pretreatment and antibiotics as selective agents. Based on preliminary examination, 21 out of 65 isolates were kept for further investigations: Planomonospora (10) and Saccharothrix/Actinophytocola (11). 16S rRNA gene sequence analysis and DNA/DNA hybridization (DDH) showed that four strains, isolated from two different sites: Béchar (PM3) and Ghardaïa (MB20 and MB27), were found to represent four novel species. Moreover, they formed a distinct phyletic line within the clade of the most related genus: PM3 (DSM 46752T) with Planomonospora, MB27 (DSM 46885T) and MB46 (DSM 46886T) with Saccharothrix and MB20 (DSM 46746T) with Actinophytocola. While many novel species belonging to the genera Saccharothrix have been isolated from Algerian Saharan soils, this is the first report to describe the isolation of new species of Planomonospora and Actinophytocola from this extreme habitat. An assessment of the antimicrobial properties of the actinobacterial strains showed that 38 out of 65 have moderate to strong antimicrobial activities against Gram positive bacteria, fungi or yeasts. These results indicated the importance of further exploration of rare Saharan Actinobacteria for potentially original antimicrobial agents. [ABSTRACT FROM AUTHOR]

Published
2018
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