42 results on '"Chaohong, Wang"'
Search Results
2. Development and clinical evaluation of a real-time multiple cross displacement amplification assay for rapid and sensitive detection of Mycobacterium tuberculosis
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Chunrong Sun, Chaohong Wang, Fei Xiao, Nan Jia, Xiaolan Huang, Jin Fu, Yu Zhang, Juan Zhou, Guirong Wang, and Yi Wang
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Mycobacterium tuberculosis ,Real-time ,Multiple cross displacement amplification ,Rapid diagnosis ,Lateral flow biosensor ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Molecular techniques of nucleic acid testing recommended by the World Health Organization (WHO) for the Mycobacterium tuberculosis (MTB) detection were considered to have the potential access to the accurate tuberculosis (TB) notifications. In this study, a new method, which coupled real-time (rt) fluorescence technique with multiple cross displacement amplification (MCDA), was developed for the rapid, sensitive and specific detection of MTB (termed MTB-rt-MCDA). According to the principle of the rt-MCDA test, a set of ten primers were designed for the MCDA reaction, of which one was engineered with a restrictive endonuclease recognition site, a fluorophore and a quencher for achieving the real-time fluorescence detection. MTB-rt-MCDA test was conducted under the optimized conditions (67 °C, 40 min) on the real-time fluorescence platform. The MTB-rt-MCDA assay accurately identified the MTB strains with no cross reaction with other bacteria. The lowest detectable genomic DNA concentration of the MTB-rt-MCDA assay was 25 fg/μl. We employed the genomic DNA templates extracted from sputum of clinical cases for validating the practical applicability of this assay, and the detection power of the MTB-rt-MCDA assay was comparable to that of the Xpert method and MCDA-based biosensor detection and superior to smear microscope method. The complete process of the MTB-rt-MCDA assay, including rapid extraction of DNA and rt-MCDA test, takes less than 1 h. In conclusion, the presented MTB-rt-MCDA assay provided an effective and simple option for the rapid screening of MTB infection.
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- 2024
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3. Application of non-invasive prenatal testing to 91,280 spontaneous pregnancies and 3477 pregnancies conceived by in vitro fertilization
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Rong Wei, Jingran Li, Yuanyuan Xia, Chaohong Wang, Xinran Lu, Yuqin Fang, and Jiansheng Zhu
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Non-invasive prenatal testing (NIPT) ,Assisted reproductive technology (ART) ,Cell-free DNA ,In vitro fertilization (IVF) ,Sex chromosome aneuploidy (SCA) ,Chromosomal aneuploidies ,Genetics ,QH426-470 - Abstract
Abstract Background Many clinical studies based on spontaneous pregnancies (SPs) have demonstrated the superiority of non-invasive prenatal testing (NIPT), and the question of whether this technology is suitable for offspring conceived by assisted reproductive technology has attracted attention. This study aimed to evaluate the application value of NIPT in screening for trisomy (T)21, T18, T13 and sex chromosome aneuploidy (SCA) in pregnant women who conceived by in vitro fertilization (IVF). Results In total, there were 804 high-risk cases [0.88% (804/91280), singleton = 795, twin = 9] in the SP group. Among the 558 invasive prenatal diagnosis (IPD) cases (singleton = 556, twin = 2), 343 (singleton = 342, twin = 1) were true positive, including 213 cases of T21, 28 of T18, 5 of T13 and 97 (singleton = 96, twin = 1) of SCA. The positive predictive values (PPVs) of T21, T18, T13, SCA and T21/T18/T13 combined in singleton pregnancy were 89.12% (213/239), 51.85% (28/54), 21.74% (5/23), 40.00% (96/240), and 77.85% (246/316), respectively, and the PPV of SCA in twin pregnancy was 100.00%. In the IVF group, IPD was performed in 19 (singleton = 16, twin = 3) of the 27 high-risk cases [0.78% (27/3477), singleton = 16, twin = 3], of which 9 (singleton = 8, twin = 1) were true positive, including 5 cases (singleton = 4, twin = 1) of T21 and 4 of SCA. The PPVs of singleton T21, SCA and T21/T18/T13 combined were 66.67% (4/6), 50.00% (4/8) and 57.14% (4/7), respectively, and the PPV of twin T21 was 100.00% (1/1). There were no significant differences in PPV among T21, SCA and T21/T18/T13 combined in singletons between the groups (89.12% vs. 66.67%, p = 0.09; 40.00% vs. 50.00%, p = 0.57; 77.85% vs. 57.14%, p = 0.20). The sensitivity and specificity were higher for singleton and twin pregnancies in the two groups. Based on follow-up results, 1 case of false negative T21 was found in the singleton SP group. Additionally, the mean foetal fraction (FF) of the IVF group was lower than that of the SP group (11.23% vs. 10.51%, p
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- 2023
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4. Clinical application value of expanded carrier screening in the population of childbearing age
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Yuqin Fang, Jingran Li, Miaomiao Zhang, Yuan Cheng, Chaohong Wang, and Jiansheng Zhu
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Expanded carrier screening ,Genetic disease ,Gene ,Variant ,Medicine - Abstract
Abstract Objective The objective of this study was to explore the clinical utility of the implementation of expanded carrier screening (ECS) in Chinese population of childbearing age. Materials and methods Based on capillary electrophoresis, a first-generation sequencing technology, a prospective screening study of carriers of 15 single-gene diseases was carried out in 327 subjects in Anhui Province, including 84 couples and 159 women of childbearing age, the disease carrier rate, types of screened pathogenic genes, and incidence of both partners carrying the same pathogenic genes were summarized and analyzed. Results In 320 people with normal phenotypes who underwent ECS for 15 genetic diseases and 7 spouses who underwent targeted gene sequencing, 65 carriers of at least one disease were detected, with a total carrier rate of 20.31% (65/320). Among the 65 carriers, 81.54% (53/65) carried one genetic variant, 16.92% (11/65) carried two genetic variants, and 1.54% (1/65) carried three genetic variants. In this study, the three diseases with the highest carrier rates were hereditary deafness (8.13%, 26/320), Wilson's disease (4.06%, 13/320), and phenylketonuria (3.13%, 10/320). One high-risk couple (1.19%, 1/84) was detected. Conclusions It has certain clinical application value to implement ECS in the population of childbearing age in China.
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- 2023
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5. Multivariate Evaluation of Photovoltaic Utilization Potential of Primary and Secondary School Buildings: A Case Study in Hainan Province, China
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Chaohong Wang, Xudong Zhang, Wang Chen, Feihu Jiang, and Xiaogang Zhao
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solar energy ,energy balance ,zero energy building ,building-attached photovoltaics ,analysis of photovoltaic potential ,Building construction ,TH1-9745 - Abstract
Modernization and industrialization have significantly increased energy consumption, causing environmental problems. Given that China is the largest energy user, the rise in building energy consumption necessitates clean energy alternatives. The purpose of this study is to summarize typical building models for primary and secondary schools in Hainan Province, and to use software to simulate and calculate the photovoltaic utilization potential of primary and secondary school buildings. In China, the government is usually the manager of primary and secondary schools, and due to their architectural characteristics, these buildings can be used to assess photovoltaic applications. The aim is to drive the application of photovoltaic systems in all types of buildings and promote urban energy reform. This study summarizes the types of primary and secondary school buildings in Hainan Province and analyzes them. It evaluates rooftop photovoltaic projects at the Second Middle School and the Siyuan School in Wanning City, Hainan Province, and uses PVsyst 7.2 software to assess the photovoltaic utilization potential. The results show that the optimal orientation in Hainan Province is south-facing, and the optimal inclination angle is 10° to 20°. The most favorable orientations of facade photovoltaic systems are 20° southeast or southwest. The longest dynamic investment payback period is approximately 15 years, and the environmental benefits are $0.012/kWh. The findings indicate significant potential for photovoltaic applications in primary and secondary school buildings. A combination of facade and rooftop photovoltaics can result in the zero-energy consumption of these buildings, reducing the pressure on urban power grids and achieving sustainable utilization.
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- 2024
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6. Single-cell profiling reveals distinct immune response landscapes in tuberculous pleural effusion and non-TPE
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Xinting Yang, Jun Yan, Yu Xue, Qing Sun, Yun Zhang, Ru Guo, Chaohong Wang, Xuelian Li, Qingtao Liang, Hangyu Wu, Chong Wang, Xinlei Liao, Sibo Long, Maike Zheng, Rongrong Wei, Haoran Zhang, Yi Liu, Nanying Che, Laurence Don Wai Luu, Junhua Pan, Guirong Wang, and Yi Wang
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Mycobacterium tuberculosis ,tuberculosis ,tuberculous pleural effusion ,ScRNA-seq ,local immune response ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundTuberculosis (TB) is caused by Mycobacterium tuberculosis (Mtb) and remains a major health threat worldwide. However, a detailed understanding of the immune cells and inflammatory mediators in Mtb-infected tissues is still lacking. Tuberculous pleural effusion (TPE), which is characterized by an influx of immune cells to the pleural space, is thus a suitable platform for dissecting complex tissue responses to Mtb infection.MethodsWe employed singe-cell RNA sequencing to 10 pleural fluid (PF) samples from 6 patients with TPE and 4 non-TPEs including 2 samples from patients with TSPE (transudative pleural effusion) and 2 samples with MPE (malignant pleural effusion).ResultCompared to TSPE and MPE, TPE displayed obvious difference in the abundance of major cell types (e.g., NK, CD4+T, Macrophages), which showed notable associations with disease type. Further analyses revealed that the CD4 lymphocyte population in TPE favored a Th1 and Th17 response. Tumor necrosis factors (TNF)-, and XIAP related factor 1 (XAF1)-pathways induced T cell apoptosis in patients with TPE. Immune exhaustion in NK cells was an important feature in TPE. Myeloid cells in TPE displayed stronger functional capacity for phagocytosis, antigen presentation and IFN-γ response, than TSPE and MPE. Systemic elevation of inflammatory response genes and pro-inflammatory cytokines were mainly driven by macrophages in patients with TPE.ConclusionWe provide a tissue immune landscape of PF immune cells, and revealed a distinct local immune response in TPE and non-TPE (TSPE and MPE). These findings will improve our understanding of local TB immunopathogenesis and provide potential targets for TB therapy.
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- 2023
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7. A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application
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Nan Jia, Chaohong Wang, Xiaming Liu, Xiaolan Huang, Fei Xiao, Jin Fu, Chunrong Sun, Zheng Xu, Guirong Wang, Juan Zhou, and Yi Wang
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Mycobacterium tuberculosis ,multiple cross displacement amplification ,CRISPR ,Cas12a ,tuberculosis ,Microbiology ,QR1-502 - Abstract
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platform for tuberculosis diagnosis, termed MTB-MCDA-CRISPR. MTB-MCDA-CRISPR pre-amplified the specific sdaA gene of MTB by MCDA, and the MCDA results were then decoded by CRISPR-Cas12a-based detection, resulting in simple visual fluorescent signal readouts. A set of standard MCDA primers, an engineered CP1 primer, a quenched fluorescent ssDNA reporter, and a gRNA were designed targeting the sdaA gene of MTB. The optimal temperature for MCDA pre-amplification is 67°C. The whole experiment process can be completed within one hour, including sputum rapid genomic DNA extraction (15 minutes), MCDA reaction (40 minutes), and CRISPR-Cas12a-gRNA biosensing process (5 minutes). The limit of detection (LoD) of the MTB-MCDA-CRISPR assay is 40 fg per reaction. The MTB-MCDA-CRISPR assay does not cross reaction with non-tuberculosis mycobacterium (NTM) strains and other species, validating its specificity. The clinical performance of MTB-MCDA-CRISPR assay was higher than that of the sputum smear microscopy test and comparable to that of Xpert method. In summary, the MTB-MCDA-CRISPR assay is a promising and effective tool for tuberculosis infection diagnosis, surveillance and prevention, especially for point-of-care (POC) test and field deployment in source-limited regions.
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- 2023
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8. Expanding the application of non-invasive prenatal testing in the detection of foetal chromosomal copy number variations
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Chaohong Wang, Junxiang Tang, Keting Tong, Daoqi Huang, Huayu Tu, Qingnan Li, and Jiansheng Zhu
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Non-invasive prenatal testing (NIPT) ,Chromosomal aneuploidy ,Chromosomal microdeletion/microduplication ,Copy number variation (CNV) ,Chromosomal microarray analysis (CMA) ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Purpose The aim of this study was to assess the detection efficiency and clinical application value of non-invasive prenatal testing (NIPT) for foetal copy number variants (CNVs) in clinical samples from 39,002 prospective cases. Methods A total of 39,002 pregnant women who received NIPT by next-generation sequencing (NGS) with a sequencing depth of 6 M reads in our centre from January 2018 to April 2020 were enrolled. Chromosomal microarray analysis (CMA) was further used to diagnose suspected chromosomal aneuploidies and chromosomal microdeletion/microduplication for consistency assessment. Results A total of 473 pregnancies (1.213%) were positive for clinically significant foetal chromosome abnormalities by NIPT. This group comprised 99 trisomy 21 (T21, 0.254%), 30 trisomy 18 (T18, 0.077%), 25 trisomy 13 (T13, 0.064%), 155 sex chromosome aneuploidy (SCA, 0.398%), 69 rare trisomy (0.177%), and 95 microdeletion/microduplication syndrome (MMS, 0.244%) cases. Based on follow-up tests, the positive predictive values (PPVs) for the T21, T18, T13, SCA, rare trisomy, and MMS cases were calculated to be 88.89%, 53.33%, 20.00%, 40.22%, 4.88%, and 49.02%, respectively. In addition, the PPVs of CNVs of 10 Mb were 54.55%, 38.46%, and 40.00%, respectively. Among the 95 cases with suspected CNVs, 25 were diagnosed as true positive and 26 cases as false positive; follow-up prenatal diagnosis by CMA was not performed for 44 cases. Moreover, among the 25 true positive cases, 10 were pathogenic, 3 were likely pathogenic, and 12 were of uncertain significance. Conclusion NIPT is not only suitable for screening T21, T18, T13, and SCA but also has potential significance for CNV detection. As combined with ultrasound, extended NIPT is effective for screening MMS. However, NIPT should not be recommended for whole-chromosome aneuploidy screening.
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- 2021
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9. Trends and Species Diversity of Non-tuberculous Mycobacteria Isolated From Respiratory Samples in Northern China, 2014–2021
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Qing Sun, Jun Yan, Xinlei Liao, Chaohong Wang, Chenqian Wang, Guanglu Jiang, Lingling Dong, Fen Wang, Hairong Huang, Guirong Wang, and Junhua Pan
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mycobacterium ,non-tuberculous mycobacteria ,species ,identification ,M. intracellulare ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundPulmonary non-tuberculous mycobacteria (NTM) infection has become a public health concern in China and around the world. The objective of this study was to describe the longitudinal changes in the frequency and diversity of NTM in northern China.MethodsWe retrospectively analyzed data on mycobacterium species in Beijing Chest Hospital from January 2014 to December 2021. The isolates were identified to species level by targeted DNA sequencing.ResultsAfter excluding duplicates, 1,755 NTM strains were analyzed, which were from 27 provinces in China over 8 years. Among all mycobacteria, the proportion of NTM increased each year, from 4.24% in 2014 to 12.68% in 2021. Overall, 39 different NTM species were identified, including 23 slow growing mycobacteria (SGM) and 16 rapid growing mycobacteria (RGM). The most common species were M. intracellulare (51.62%), M. abscessus (22.22%), M. kansasii (8.32%), M. avium (7.75%) and M. fortuitum (2.05%). The number of NTM species identified also increased each year from 9 in 2014 to 26 in 2021. Most species showed stable isolation rates over the years; however, the proportion of M. avium increased from 3.85 to 10.42% during the study period. Besides, 81 non-mycobacteria strains, including Gordonia (21 isolates), Nocardia (19 isolates) and Tsukamurella (17 isolates), etc., were also discovered.ConclusionThe proportion of NTM and species diversity increased considerably in northern China from 2014 to 2021. M. intracellulare was the most common NTM isolated among respiratory specimens, followed by M. abscessus and M. kansasii. Rare NTM species and non-mycobacteria pathogens also need attention.
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- 2022
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10. Clinical application of noninvasive prenatal testing in the detection of fetal chromosomal diseases
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Yu Pang, Chaohong Wang, Junxiang Tang, and Jiansheng Zhu
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Noninvasive prenatal testing ,Chromosome aneuploidy ,Copy number variation ,Chromosomal microarray analysis ,Genetics ,QH426-470 - Abstract
Abstract Objective To assess the detection efficiency of noninvasive prenatal testing (NIPT) for fetal autosomal aneuploidy, sex chromosome aneuploidy (SCA), other chromosome aneuploidy, copy number variation (CNV), and to provide further data for clinical application of NIPT. Materials and methods 25,517 pregnant women who underwent NIPT testing in Anhui Province Maternity and Child Health Hospital from September 2019 to September 2020 were selected, and samples with high-risk test results were subjected to karyotype analysis for comparison by using amniotic fluid, with some samples subjected to further validation by chromosomal microarray analysis, and followed up for pregnancy outcome. Results A total of 25,517 pregnant women who received NIPT, 25,502 cases were tested successfully, and 294 high-risk samples (1.15%) were detected, there were 96 true positive samples, 117 false positive samples and 81 cases were refused further diagnosis. Samples with high risk of autosomal aneuploidy were detected in 71 cases (0.28%), and 51 cases were confirmed, including: trisomy 21 (T21) in 44 cases, trisomy 18 (T18) in 5 cases, and trisomy 13 (T13) in 2 cases; the positive predictive value (PPV) was 91.67%, 45.45%, and 33.33%, respectively, and the negative predictive value was 100%, the false positive rate (FPR) was 0.02%, 0.02%, and 0.02%, respectively.13 samples with high risk of mosaic trisomies 21, 18, and 13 were detected, and 1 case of T21mos was confirmed with a PPV of 8.33%. Samples with high risk of SCA were detected in 72 cases (0.28%), and the diagnosis was confirmed in 23 cases, with a PPV of 41.07% and a FPR of 0.13%. These included 3 cases of 45,X, 6 cases of 47,XXY, 8 cases of 47,XXX and 6 cases of 47,XYY, with PPVs of 12.00%, 50.00%, 72.73%, and 75.00%, respectively, and false-positive rates of 0.09%, 0.02%, 0.01% and 0.01% respectively. Samples with high risk of CNV were detected in 104 cases (0.41%) and confirmed in 18 cases, with a PPV of 32.14% and a FPR of 0.15%. Samples with high risk of other chromosomal aneuploidy were detected in 34 cases (0.13%), and the diagnosis was confirmed in 3 cases, which were T2, T9, and T16 respectively. The overall PPV for other chromosome aneuploidy was 12.50%, with a FPR of 0.08%. Conclusion NIPT is indicated for trisomies 21, 18 and 13 screening, especially for T21. It also has some certain reference value for SCA and CNV, but is not recommended for screening of other chromosomal aneuploidy.
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- 2021
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11. Performance of non-invasive prenatal testing for foetal chromosomal abnormalities in 1048 twin pregnancies
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Yuan Cheng, Xinran Lu, Junxiang Tang, Jingran Li, Yuxiu Sun, Chaohong Wang, and Jiansheng Zhu
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Non-invasive prenatal testing ,Chromosomal aneuploidy ,Microdeletion ,Microduplication ,Twin pregnancy ,Genetics ,QH426-470 - Abstract
Abstract Objective To investigate the clinical value of non-invasive prenatal testing (NIPT) to screen for chromosomal abnormalities in twin pregnancies and to provide further data on NIPT manifestations in twin pregnancies. Materials and methods In a 4-year period, 1048 women with twin pregnancies were voluntarily prospectively tested by NIPT to screen for chromosomal abnormalities by sequencing cell-free foetal DNA (cffDNA) in maternal plasma. Positive NIPT results were confirmed by karyotyping, while negative results were followed up 42 days after delivery. Results Thirteen women had positive NIPT results as follows: 2 cases of trisomy 21 (T21), 1 of trisomy 18 (T18), 7 of sex chromosome aneuploidy (SCA), 1 of microdeletion, and 2 of microduplication. Of these 13 cases, 2 were true-positive cases confirmed by foetal karyotype analysis, namely, 1 case of T21 and 1 of microdeletion. Furthermore, the remaining 11 high-risk pregnant women were confirmed as false positive by foetal karyotyping. Thus, the combined positive predictive value (PPV) of NIPT screening for chromosomal abnormalities in twin pregnancies was 15.4% (2/13). There were no false-negative case via our follow-up results. Conclusion Safe and rapid NIPT has a certain clinical application value; however, the PPV is limited, and the screening efficiency is not stable. Careful use should be made in the screening of chromosomal abnormalities in twin pregnancies.
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- 2021
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12. Noninvasive prenatal testing for assessing foetal sex chromosome aneuploidy: a retrospective study of 45,773 cases
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Xinran Lu, Chaohong Wang, Yuxiu Sun, Junxiang Tang, Keting Tong, and Jiansheng Zhu
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Noninvasive prenatal testing ,Sex chromosome aneuploidy ,Positive predictive value ,Advanced maternal age ,High-throughput sequencing ,Prenatal screening ,Genetics ,QH426-470 - Abstract
Abstract Objective To assess the positive predictive value (PPV) of noninvasive prenatal testing (NIPT) as a screening test for sex chromosome aneuploidy (SCA) with different maternal characteristics and prenatal decisions in positive cases. Materials and methods We retrospectively analysed 45,773 singleton pregnancies with different characteristics that were subjected to NIPT in the Maternity and Child Health Hospital of Anhui Province. The results were validated by karyotyping. Clinical data, diagnostic results, and data on pregnancy outcomes were collected. Results In total, 314 cases were SCA positive by NIPT; among those, 143 underwent invasive prenatal diagnostic testing, and 58 were true-positive. Overall, the PPVs for 45,X, 47,XXX, 47,XXY and 47,XYY were 12.5%, 51.72%, 66.67% and 83.33%, respectively. Interestingly, when only pregnant women of advanced maternal age (AMA) were screened, the PPVs for 45,X, 47,XXX, 47,XXY and 47,XYY were 23.81%, 53.33%, 78.95%, and 66.67%, respectively. The frequency of SCA was significantly higher in the AMA group than in the non-AMA group. The frequencies of 47,XXX and 47,XXY were significantly correlated with maternal age. Conclusion NIPT performed better in predicting sex chromosome trisomies than monosomy X, and patients with 45,X positive foetuses were more eager to terminate pregnancy than those with 47,XXX and 47,XYY. AMA may be a risk factor of having a foetus with SCA. Our findings may assist in genetic counselling of AMA pregnant women. Our pre- and posttest counselling are essential for familiarizing pregnant women with the benefits and limitations of NIPT, which may ease their anxiety and enable them to make informed choices for further diagnosis and pregnancy decisions.
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- 2021
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13. Value of noninvasive prenatal testing in the detection of rare fetal autosomal abnormalities
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Miaomiao Zhang, Junxiang Tang, Jingran Li, Chaohong Wang, Rong Wei, Yuqin Fang, and Jiansheng Zhu
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Reproductive Medicine ,Obstetrics and Gynecology - Published
- 2023
14. High‐resolution multi‐shot diffusion‐weighted <scp>MRI</scp> combining markerless prospective motion correction and locally low‐rank constrained reconstruction
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Hao Chen, Ke Dai, Sijie Zhong, Jiaxu Zheng, Xinyue Zhang, Shasha Yang, Tuoyu Cao, Chaohong Wang, Ekin Karasan, Lucio Frydman, and Zhiyong Zhang
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Adult ,Echo-Planar Imaging ,Infant, Newborn ,Brain ,Motion ,Diffusion Magnetic Resonance Imaging ,Image Interpretation, Computer-Assisted ,Image Processing, Computer-Assisted ,Humans ,Radiology, Nuclear Medicine and imaging ,Prospective Studies ,Child ,Artifacts ,Algorithms ,Retrospective Studies - Abstract
Subject head motion is a major challenge in DWI, leading to image blurring, signal losses, and biases in the estimated diffusion parameters. Here, we investigate a combined application of prospective motion correction and spatial-angular locally low-rank constrained reconstruction to obtain robust, multi-shot, high-resolution diffusion-weighted MRI under substantial motion.Single-shot EPI with retrospective motion correction can mitigate motion artifacts and resolve any mismatching of gradient encoding orientations; however, it is limited by low spatial resolution and image distortions. Multi-shot acquisition strategies could achieve higher resolution and image fidelity but increase the vulnerability to motion artifacts and phase variations related to cardiac pulsations from shot to shot. We use prospective motion correction with optical markerless motion tracking to remove artifacts and reduce image blurring due to bulk motion, combined with locally low-rank regularization to correct for remaining artifacts due to shot-to-shot phase variations.The approach was evaluated on healthy adult volunteers at 3 Tesla under different motion patterns. In multi-shot DWI, image blurring due to motion with 20 mm translations and 30° rotations was successfully removed by prospective motion correction, and aliasing artifacts caused by shot-to-shot phase variations were addressed by locally low-rank regularization. The ability of prospective motion correction to preserve the orientational information in DTI without requiring a reorientation of the b-matrix is highlighted.The described technique is proved to hold valuable potential for mapping brain diffusivity and connectivity at high resolution for studies in subjects/cohorts where motion is common, including neonates, pediatrics, and patients with neurological disorders.
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- 2022
15. Applications of non-invasive prenatal testing in 91280 spontaneous pregnancies and 3477 pregnant women undergoing in vitro Fertilization
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Rong Wei Wei, Jingran Li, Xinran Lu, Miaomiao Zhang, Yuqin Fang, Yuanyuan Xia, Chaohong Wang, and Jiansheng Zhu
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Background A large number of clinical studies based on spontaneous pregnancies (SP) have proved the superiority of non-invasive prenatal testing (NIPT), and whether this technology is suitable for the offspring of assisted reproductive technology (ART) has attracted people's attention. The aim of this study is to evaluate the application value of NIPT in screening for trisomy21(T21), trisomy18(T18), trisomy13(T13) and sex chromosome aneuploidy (SCA) in pregnant women underwent in vitro fertilization (IVF). Results A total of 804 cases (0.88% (804/91280), singleton = 795, twin = 9) of high-risk in the SP group, of the 558 cases (singleton = 556, twin = 2) that underwent invasive prenatal diagnosis (IPD), 343 cases (singleton = 342, twin = 1) were true positive, including 213 cases T21, 28 cases T18, 5 cases T13 and 97 cases (singleton = 96, twin = 1) SCA. The positive predictive value (PPV) of T21, T18, T13 and SCA in singleton pregnancies were 89.12% (213/239), 51.85% (28/54), 21.74% (5/23) and 40.00% (96/240), and the PPV of SCA in twin pregnancies was 100.00%. In the in vitro fertilization (IVF) group, IPD was underwent in 19 cases (singleton = 16, twin = 3) of the 27 cases (0.78% (27/3477), singleton = 16, twin = 3) high-risk, of which 9 cases (singleton = 8, twin = 1) were true positive, including 5 cases (singleton = 4, twin = 1) T21 and 4 cases of SCA. The PPV of singleton T21 and SCA was 66.67% (4/6) and 50.00% (4/8), and the PPV of twin T21 was 100.00% (1/1). There was no significant difference in sensitivity and specificity between the two groups. Based on follow-up, we found 1 case of T21 false negative results in SP group and no false negative case in IVF group. Additionally, the mean fetal fraction (FF) of IVF group was lower than that of SP group (11.23%vs.10.51%, p
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- 2023
16. Clinical Characteristics and Outcomes of Patients With COVID-19 And Active Tuberculosis Co-Infection in Beijing, China: A Retrospective, Single-Centre, Descriptive Study
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Xinting Yang, Chaohong Wang, Yu Xue, Yun Zhang, Maike Zheng, Qing Sun, Sibo Long, Da Wang, Jun Yan, Xinlei Liao, Tiantian Zhang, Lei Cao, Yan Chen, Wenfeng Ju, Jing Zhang, Mengqiu Gao, Yan Zhao, Laurence Don Wai Luu, Junhua Pan, Yi Wang, and Guirong Wang
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- 2023
17. Noninvasive prenatal testing for assessing foetal sex chromosome aneuploidy: a retrospective study of 45,773 cases
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Jiansheng Zhu, Keting Tong, Xinran Lu, Junxiang Tang, Chaohong Wang, and Yuxiu Sun
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medicine.medical_specialty ,Positive predictive value ,lcsh:QH426-470 ,Genetic counseling ,Prenatal diagnosis ,Noninvasive prenatal testing ,Advanced maternal age ,Biochemistry ,Genetics ,medicine ,Risk factor ,Molecular Biology ,Genetics (clinical) ,Pregnancy ,Fetus ,High-throughput sequencing ,Obstetrics ,business.industry ,Research ,Biochemistry (medical) ,Retrospective cohort study ,Prenatal screening ,medicine.disease ,lcsh:Genetics ,Sex chromosome aneuploidy ,Molecular Medicine ,Anxiety ,medicine.symptom ,business - Abstract
Objective To assess the positive predictive value (PPV) of noninvasive prenatal testing (NIPT) as a screening test for sex chromosome aneuploidy (SCA) with different maternal characteristics and prenatal decisions in positive cases. Materials and methods We retrospectively analysed 45,773 singleton pregnancies with different characteristics that were subjected to NIPT in the Maternity and Child Health Hospital of Anhui Province. The results were validated by karyotyping. Clinical data, diagnostic results, and data on pregnancy outcomes were collected. Results In total, 314 cases were SCA positive by NIPT; among those, 143 underwent invasive prenatal diagnostic testing, and 58 were true-positive. Overall, the PPVs for 45,X, 47,XXX, 47,XXY and 47,XYY were 12.5%, 51.72%, 66.67% and 83.33%, respectively. Interestingly, when only pregnant women of advanced maternal age (AMA) were screened, the PPVs for 45,X, 47,XXX, 47,XXY and 47,XYY were 23.81%, 53.33%, 78.95%, and 66.67%, respectively. The frequency of SCA was significantly higher in the AMA group than in the non-AMA group. The frequencies of 47,XXX and 47,XXY were significantly correlated with maternal age. Conclusion NIPT performed better in predicting sex chromosome trisomies than monosomy X, and patients with 45,X positive foetuses were more eager to terminate pregnancy than those with 47,XXX and 47,XYY. AMA may be a risk factor of having a foetus with SCA. Our findings may assist in genetic counselling of AMA pregnant women. Our pre- and posttest counselling are essential for familiarizing pregnant women with the benefits and limitations of NIPT, which may ease their anxiety and enable them to make informed choices for further diagnosis and pregnancy decisions.
- Published
- 2021
18. Chromosomal microarray analysis versus noninvasive prenatal testing in fetuses with increased nuchal translucency
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Chaohong Wang, Junxiang Tang, Keting Tong, Daoqi Huang, Huayu Tu, and Jiansheng Zhu
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Chromosome Aberrations ,Fetus ,DNA Copy Number Variations ,Pregnancy ,Noninvasive Prenatal Testing ,Prenatal Diagnosis ,Karyotype ,Genetics ,Humans ,Female ,Microarray Analysis ,Nuchal Translucency Measurement ,Genetics (clinical) - Abstract
Objective To evaluate if the NT value of 2.5 mm ≤ NT Methods A total of 442 fetuses with NT ≥ 2.5 mm were included, in which 241 fetuses underwent karyotype. CMA tests were then carried out when cytogenic analysis showed normal chromosomes and CNV status was compared between 2.5 mm ≤ NT Results Of the 241 fetuses with NT ≥ 2.5 mm, 47(19.50%) were identified by karyotype with chromosomal abnormalities. Among 194 cases with normal karyotype, CMA unraveled additional CNVs in 16(8.25%) cases, including 3(1.55%) pathogenic CNVs, 2(1.03%) likely pathogenic CNVs and 11(5.67%) VOUS. After the subgroup analysis, however, only one case (1.16%) of likely pathogenic was identified by CMA among 86 fetuses with NT between 2.5 mm and 3.0 mm, whereas the rest of 15 CNV cases were all presented in fetuses with NT ≥ 3.0 mm. For the NIPT evaluation, the detection rate of 201 fetuses with isolated increased NT between 2.5 and 3.0 mm was 3.98%, which was indifferent to karyotype with the rate of 5%. In comparison with fetuses with 2.5–3.0 mm combined with other risks, the detection rate of karyotype was 26.92%. Conclusion While no pathogenic CNVs were detected in fetuses, chromosomal aneuploidies and genomic imbalance were found to be the major type of abnormalities when NT was 2.5–3.0 mm. Therefore, our data suggested that CMA should not be recommended when fetuses with an NT value less than 3.0 mm. Instead, NIPT with similar rate of detection as karyotype was recommended for fetuses with isolated increased NT between 2.5 and 3.0 mm.
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- 2022
19. Classical nucleation theory of ice nucleation: Second-order corrections to thermodynamic parameters
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Chaohong Wang, Hao Wang, Zhisen Zhang, and Jianyang Wu
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Materials science ,010304 chemical physics ,Nucleation ,General Physics and Astronomy ,Thermodynamics ,FOS: Physical sciences ,Condensed Matter - Soft Condensed Matter ,010402 general chemistry ,01 natural sciences ,0104 chemical sciences ,law.invention ,Molecular dynamics ,Order (biology) ,Potential difference ,law ,0103 physical sciences ,Ice nucleus ,Soft Condensed Matter (cond-mat.soft) ,Classical nucleation theory ,Physical and Theoretical Chemistry ,Crystallization ,Physics::Atmospheric and Oceanic Physics - Abstract
Accurate estimate of nucleation rate is crucial for the study of ice nucleation and ice-promoting/anti-freeze strategies. Within the framework of Classical Nucleation Theory (CNT), the estimate of ice nucleation rate is very sensitive to thermodynamic parameters, such as chemical potential difference between water and ice $\Delta \mu$ and ice-water interfacial free energy $\gamma$. However, even today, there are still many contradictions and approximations in the estimating of these thermodynamic parameters, introducing large uncertainty to the estimate of the ice nucleation rate. Herein, starting from the basic concepts, for a general solid-liquid crystallization system, we expand the Gibbs-Thomson (GT) equation to second order, and derive the second-order analytical formulas of $\Delta \mu$, $\gamma$ and nucleation barrier $\Delta G$ with combining molecular dynamics (MD) simulations. These formulas describe well the temperature dependence of these thermodynamic parameters. Our results can provide a method of estimating $\Delta \mu$, $\gamma$ and $\Delta G$., Comment: 6 pages, 4 figures
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- 2021
20. Performance of non-invasive prenatal testing for foetal chromosomal abnormalities in 1048 twin pregnancies
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Yuxiu Sun, Xinran Lu, Jingran Li, Chaohong Wang, Jiansheng Zhu, Junxiang Tang, and Yuan Cheng
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0301 basic medicine ,medicine.medical_specialty ,Twin pregnancy ,Microduplication ,QH426-470 ,030105 genetics & heredity ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Molecular Biology ,Genetics (clinical) ,Twin Pregnancy ,Chromosomal aneuploidy ,030219 obstetrics & reproductive medicine ,business.industry ,Obstetrics ,Research ,Biochemistry (medical) ,Non invasive ,Cytogenetics ,Karyotype ,medicine.disease ,Predictive value ,Non-invasive prenatal testing ,Cell-free fetal DNA ,Clinical value ,Microdeletion ,Molecular Medicine ,Trisomy ,business - Abstract
Objective To investigate the clinical value of non-invasive prenatal testing (NIPT) to screen for chromosomal abnormalities in twin pregnancies and to provide further data on NIPT manifestations in twin pregnancies. Materials and methods In a 4-year period, 1048 women with twin pregnancies were voluntarily prospectively tested by NIPT to screen for chromosomal abnormalities by sequencing cell-free foetal DNA (cffDNA) in maternal plasma. Positive NIPT results were confirmed by karyotyping, while negative results were followed up 42 days after delivery. Results Thirteen women had positive NIPT results as follows: 2 cases of trisomy 21 (T21), 1 of trisomy 18 (T18), 7 of sex chromosome aneuploidy (SCA), 1 of microdeletion, and 2 of microduplication. Of these 13 cases, 2 were true-positive cases confirmed by foetal karyotype analysis, namely, 1 case of T21 and 1 of microdeletion. Furthermore, the remaining 11 high-risk pregnant women were confirmed as false positive by foetal karyotyping. Thus, the combined positive predictive value (PPV) of NIPT screening for chromosomal abnormalities in twin pregnancies was 15.4% (2/13). There were no false-negative case via our follow-up results. Conclusion Safe and rapid NIPT has a certain clinical application value; however, the PPV is limited, and the screening efficiency is not stable. Careful use should be made in the screening of chromosomal abnormalities in twin pregnancies.
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- 2021
21. Clinical application of noninvasive prenatal testing in the detection of fetal chromosomal diseases
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Chaohong Wang, Jiansheng Zhu, Junxiang Tang, and Yu Pang
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medicine.medical_specialty ,Amniotic fluid ,Chromosome aneuploidy ,Noninvasive prenatal testing ,Aneuploidy ,QH426-470 ,Biochemistry ,Genetics ,medicine ,Copy-number variation ,Molecular Biology ,Genetics (clinical) ,Pregnancy ,Copy number variation ,Obstetrics ,business.industry ,Research ,Biochemistry (medical) ,Cytogenetics ,Karyotype ,medicine.disease ,Molecular Medicine ,False positive rate ,Trisomy ,business ,Chromosomal microarray analysis - Abstract
Objective To assess the detection efficiency of noninvasive prenatal testing (NIPT) for fetal autosomal aneuploidy, sex chromosome aneuploidy (SCA), other chromosome aneuploidy, copy number variation (CNV), and to provide further data for clinical application of NIPT. Materials and methods 25,517 pregnant women who underwent NIPT testing in Anhui Province Maternity and Child Health Hospital from September 2019 to September 2020 were selected, and samples with high-risk test results were subjected to karyotype analysis for comparison by using amniotic fluid, with some samples subjected to further validation by chromosomal microarray analysis, and followed up for pregnancy outcome. Results A total of 25,517 pregnant women who received NIPT, 25,502 cases were tested successfully, and 294 high-risk samples (1.15%) were detected, there were 96 true positive samples, 117 false positive samples and 81 cases were refused further diagnosis. Samples with high risk of autosomal aneuploidy were detected in 71 cases (0.28%), and 51 cases were confirmed, including: trisomy 21 (T21) in 44 cases, trisomy 18 (T18) in 5 cases, and trisomy 13 (T13) in 2 cases; the positive predictive value (PPV) was 91.67%, 45.45%, and 33.33%, respectively, and the negative predictive value was 100%, the false positive rate (FPR) was 0.02%, 0.02%, and 0.02%, respectively.13 samples with high risk of mosaic trisomies 21, 18, and 13 were detected, and 1 case of T21mos was confirmed with a PPV of 8.33%. Samples with high risk of SCA were detected in 72 cases (0.28%), and the diagnosis was confirmed in 23 cases, with a PPV of 41.07% and a FPR of 0.13%. These included 3 cases of 45,X, 6 cases of 47,XXY, 8 cases of 47,XXX and 6 cases of 47,XYY, with PPVs of 12.00%, 50.00%, 72.73%, and 75.00%, respectively, and false-positive rates of 0.09%, 0.02%, 0.01% and 0.01% respectively. Samples with high risk of CNV were detected in 104 cases (0.41%) and confirmed in 18 cases, with a PPV of 32.14% and a FPR of 0.15%. Samples with high risk of other chromosomal aneuploidy were detected in 34 cases (0.13%), and the diagnosis was confirmed in 3 cases, which were T2, T9, and T16 respectively. The overall PPV for other chromosome aneuploidy was 12.50%, with a FPR of 0.08%. Conclusion NIPT is indicated for trisomies 21, 18 and 13 screening, especially for T21. It also has some certain reference value for SCA and CNV, but is not recommended for screening of other chromosomal aneuploidy.
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- 2021
22. The Clinical Application of Non-invasive Prenatal Genetic Testing in the Screening of Fetal Chromosomal Diseases
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Jiansheng Zhu, Chaohong Wang, Junxiang Tang, and Yu Pang
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Fetus ,Text mining ,genetic structures ,medicine.diagnostic_test ,business.industry ,Non invasive ,medicine ,business ,Bioinformatics ,Chromosomal diseases ,Genetic testing - Abstract
Objective:To explore the efficacy and clinical application value of non-invasive prenatal testing (non-invasive prenatal testing, NIPT) for screening fetal chromosomal abnormalities. Methods: NIPT was performed on 25,517 pregnant women. The high-risk samples were compared with amniotic fluid and cord blood chromosome karyotype analysis. Some samples were further verified by microarray (CMA), and pregnancy outcomes were followed up. Results: Of all the cases examined, 25502 cases were detected successfully, and a total of 294 high-risk samples (1.15%) were detected, of which further diagnosis was made in 208 cases, true positive samples were detected in 96 cases, and further tests were refused in 86 cases.71 cases (0.28%) of autosomal aneuploid high-risk samples were detected and 51 cases were diagnosed, including 44 cases of trisomy 21 (T21), 5 cases of trisomy 18 (T18), and 2 cases of trisomy 13 (T13). The PPV was 90.90%, 45.45% and 33.33%, respectively. Thirteen high-risk samples of trisomy 21, 18, and 13 were detected, and 1 case was confirmed as T21 mosaic PPV was 8.33% NPV was 100%. High-risk samples of sex chromosome aneuploidy (SCA) were detected in 72 cases (0.28%), 23 cases were diagnosed, and the PPV was 40.07%. The PPV was 12.00%, 50.00%, 72.73% and 75.00%, respectively, and the PPV was 12.00%, 50.00%, 72.73% and 75.00%, respectively. High-risk samples of copy number variation (CNV) were detected in 104 cases (0.41%), and 18 cases were diagnosed, with a PPV of 32.14%. Other high-risk samples of chromosome aneuploidy were detected in 34 cases (0.13%), and 3 cases were diagnosed as T2, T9, and T16, respectively. PPV is 8.70%.Conclusion: NIPT is suitable for trisomy 21, 18, and 13 screening, especially for T21. It also has a certain reference value for SCA and microdeletion and microduplication syndromes(MMS), and it is not recommended for screening for other chromosomal aneuploidies.
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- 2021
23. Effects of design parameters on rooftop photovoltaic economics in the urban environment: a case study in Melbourne, Australia
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Hongying Zhao, Rebecca Yang, Chaohong Wang, W. M. Pabasara, U. Wijeratne, Chengyang Liu, Xiaolong Xue, and Nishara Abdeen
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050210 logistics & transportation ,geography ,geography.geographical_feature_category ,Computer science ,05 social sciences ,Photovoltaic system ,0211 other engineering and technologies ,Internal rate of return ,02 engineering and technology ,Environmental economics ,Urban area ,Net present value ,Software deployment ,Urban planning ,0502 economics and business ,Sustainability ,021108 energy ,Roof - Abstract
Many researchers found high potential of adopting building photovoltaic (PV) systems in urban areas, especially on building rooftop, to improve the sustainability of urban environment. However, the optimal energy output performance and economic benefit of the PV system are affected by the usable roof area, PV array layout, and shading effect considering high city density. This study aims to understand the effects of these design parameters in the urban environment of rooftop PV’s economic performance. This study carries out a case study in the urban area of Melbourne with 90 PV designs under three shading conditions to generate 270 scenarios. Through a lifecycle cost-benefit analysis, including net present value (NPV), NPV per kW, internal return rate (IRR), and payback year, the results can help in developing a comprehensive understanding of the economic performance of rooftop PV designs that cover most of the urban areas of Melbourne. The optimal PV design scenarios for the urban environment are identified, thereby providing investors and industry professionals with useful information on value-for-money PV design. Meanwhile, the maximum shading loss that makes the PV systems financially unfeasible is investigated, and design scenarios with greatest ability to sustain the shading effect are identified. This research can also support the policy makers’ decision on the development and deployment of the roof PV systems in urban planning.
- Published
- 2019
24. Ecovillage Practice from the Perspective of the Concept of De-growth
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Hao Luo, Chaohong Wang, and Xiaopeng Yue
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Perspective (graphical) ,Environmental ethics ,Ecovillage ,Sociology - Published
- 2018
25. Microwave dielectric ceramics in (Mg1/3Sb2/3)O2-ZrO2-TiO2 system with near zero τf and low loss in a very wide range
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Lu Zhang, Chaohong Wang, Geng Wang, Qiuyun Fu, Zhiping Zheng, Fan Tian, Wei Luo, Hao Shi, and Tao Yang
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010302 applied physics ,Materials science ,Phonon ,Process Chemistry and Technology ,Analytical chemistry ,Sintering ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,01 natural sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,symbols.namesake ,Far infrared ,visual_art ,Phase (matter) ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,visual_art.visual_art_medium ,symbols ,Ceramic ,0210 nano-technology ,Raman spectroscopy ,Microwave - Abstract
A series of (ZrTi) 1- x (Mg 1/3 Sb 2/3 ) 2 x O 4 (0.04 ≤ x ≤ 0.36) ceramics were successfully synthesized through the conventional solid-state processing. Appropriate content of CuO was added as sintering aids to promote the density of ceramics. The XRD analysis revealed that the main crystalline phase of ceramics sintered at optimal temperature belonged to α-PbO 2 -type structure. Raman spectroscopy and far infrared reflectivity (FIR) spectra were employed to study the phonon modes of ceramics, which explained the relationship between microwave dielectric properties and structure. It is interesting that the τ f are near-zero (+6.6 ~ −4.6 ppm/°C) and meanwhile the Q×f are relatively high (29,000–41,800 GHz) for samples with x in a very wide range of 0.10–0.36. In this range, their dielectric constants (e r ) can be adjustable from 35.4 to 24.4. The results demonstrated this ceramic system is a potential candidate for microwave dielectric applications requiring an adjustable dielectric constants and near zero τ f .
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- 2018
26. Additional file 1 of Noninvasive prenatal testing for assessing foetal sex chromosome aneuploidy: a retrospective study of 45,773 cases
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Xinran Lu, Chaohong Wang, Yuxiu Sun, Junxiang Tang, Keting Tong, and Jiansheng Zhu
- Abstract
Additional file 1: Supplementary materials
- Published
- 2021
- Full Text
- View/download PDF
27. Combined Application of Chromosome Karyotype and Microarray Analysis in Fetus With Increased Nuchal Translucency
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Chaohong, Wang, primary, Junxiang, Tang, additional, Yuxiu, Sun, additional, Yu, Pang, additional, Keting, Tong, additional, and Jiansheng, Zhu, additional
- Published
- 2021
- Full Text
- View/download PDF
28. [Application of in situ amniocyte culture for prenatal diagnosis]
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Daoqi, Huang, Huayu, Tu, Chaohong, Wang, Yaqun, Wang, Keting, Tong, and Jiansheng, Zhu
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Chromosome Aberrations ,Pregnancy ,Karyotyping ,Prenatal Diagnosis ,Humans ,Chromosome Disorders ,Female ,Trisomy ,Amniotic Fluid ,Aneuploidy ,In Situ Hybridization, Fluorescence - Abstract
To explore the value of in situ amniocyte culture for prenatal diagnosis.2716 amniotic fluid samples were cultured in situ on slides. After the culture, the slides were stained, photographed and analyzed.All samples were successfully analyzed, with the success rates for primary culture and subculture being 98.42% and 1.58%, respectively. 224 samples (8.25%) were detected with chromosomal aberrations, which included 125 cases with trisomy 21, 31 with trisomy 18, 3 with trisomy 13, 4 with 45,X, 17 with 47,XXY, 5 with 47,XYY, 1 with 48,XXY,+18, 1 with 48,XXYY, 26 with structural chromosomal aberrations, and 11 with mosaicisms for aneuploidies.In situ amniocyte culture is stable and has a high success rate, and is capable of identifying true and false mosaicisms, which can improve the accuracy of prenatal diagnosis.
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- 2020
29. Enhancement of dielectric and magnetic properties in phase pure, dense BiFeO3 nanoceramics synthesized by spark plasma sintering techniques
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Wei Luo, Chaohong Wang, Yahui Tian, Mingzhi Zhang, Ling Zhou, Haibo Gou, Fei Xue, Yunxiang Hu, Qiuyun Fu, and Zhiping Zheng
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010302 applied physics ,Materials science ,Analytical chemistry ,Spark plasma sintering ,02 engineering and technology ,Dielectric ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,Exchange bias ,X-ray photoelectron spectroscopy ,Electrical resistivity and conductivity ,visual_art ,Phase (matter) ,0103 physical sciences ,visual_art.visual_art_medium ,Ceramic ,Electrical and Electronic Engineering ,0210 nano-technology - Abstract
Phase pure, dense BiFeO3 (BFO) ceramics with average grain sizes of ~ 110 nm, ~ 450 nm, and ~ 1.15 µm were fabricated by spark plasma sintering method. BFO ceramics exhibited grain-size-dependent magnetic properties, which ascribed to the antiferromagnetism–ferromagnetism (AFM–FM) transition. For BFO nanoceramics (~ 110 nm), such transition was much significant, and contributed to a large exchange bias field of HEB = 500 Oe at 5 K. In addition, BFO nanoceramics (~ 110 nm) exhibited lower leakage current and higher resistivity compared to the larger-grained BFO ceramics (~ 450 nm and ~ 1.15 µm). The calculated activation energies (Ea) and X-ray photoelectron spectroscopy analyses revealed the existence of different types of defects in BFO ceramics with different grain sizes.
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- 2018
30. Exchange bias in spin-glass-like NiFe2O4/BiFeO3 heterojunction at room temperature
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Chaohong Wang, Lu Zhang, Haibo Gou, Ling Zhou, Yahui Tian, Qiuyun Fu, Fei Xue, Jianbo Ai, and Sheng Wang
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Materials science ,Spin glass ,Condensed matter physics ,Heterojunction ,02 engineering and technology ,Coercivity ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Electronic, Optical and Magnetic Materials ,Magnetization ,Magnetic anisotropy ,Exchange bias ,Ferromagnetism ,0103 physical sciences ,Curie temperature ,010306 general physics ,0210 nano-technology - Abstract
In order to improve blocking tepmerature of the exchange bias effects in heterojunction composing of ferromagnetic (FM) materials and multiferroic BiFeO3 (BFO). NiFe2O4 (NFO) was selected as the FM material to form FM/BFO heterojunction for its high Curie temperature in this paper. NFO/BFO bilayer film was prepared on Pt/Ti/SiO2/Si substrate via a simple sol-gel spin-coating technique. Exchange bias field at room temperature has been observed as expected and HEB/HC was 4.5%. The M-T curves of the NFO/BFO heterostructure indicated that the magnetic anisotropy disappeared above 300 K. Furthermore, a field-dependent irreversible magnetization behavior and an exponential decay of coercive field and exchange bias field with temperature were found, which suggested a spin-glass-like state existed in the NFO/BFO heterostructure.
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- 2018
31. Structural and physical properties of Ti-doped BiFeO3 nanoceramics
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Chaohong Wang, Fei Xue, Ling Zhou, Mingzhi Zhang, Yahui Tian, Qiuyun Fu, and Haibo Gou
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010302 applied physics ,Materials science ,Process Chemistry and Technology ,Analytical chemistry ,Spark plasma sintering ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Ferroelectricity ,Grain size ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Magnetization ,Remanence ,Phase (matter) ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,Dielectric loss ,Multiferroics ,0210 nano-technology - Abstract
Enhanced ferroelectricity and magnetization have been observed in Ti-doped BiFeO 3 nanoceramics prepared using spark plasma sintering processes. X-ray diffraction and FESEM investigations suggested the formation of a pure rhombohedral phase BiFe 0.95 Ti 0.05 O 3 (BFTO) ceramics with an average grain size less than 100 nm. Low dielectric loss and conductivity were attributed to low concentration of Fe 2+ (Fe 3+ /Fe 2+ is 2.8) measured by X-ray photoelectron spectroscopy. The remanent polarization test identified the intrinsic ferroelectricity in BFTO nanoceramics. The piezo-response force microscopy measurements showed nano-scaled ferroelectric domains in BFTO nanoceramics. High remanent magnetization ( M r = 0.1 emu/g) was attributed to the collapse of the cycloidal spin structure for the substitution of Fe 3+ with Ti 4+ and the existence of nanoscaled grains less than the period (62 nm) of the spin cycloid.
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- 2018
32. Multiple Interfacial Fe3O4@BaTiO3/P(VDF-HFP) Core–Shell–Matrix Films with Internal Barrier Layer Capacitor (IBLC) Effects and High Energy Storage Density
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Chaohong Wang, Ling Zhou, Haibo Gou, Geng Wang, Dongxiang Zhou, Xiahui Tang, Qiuyun Fu, Lei Xu, Yahui Tian, and Fei Xue
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Nanocomposite ,Materials science ,02 engineering and technology ,Dielectric ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Energy storage ,0104 chemical sciences ,law.invention ,Barrier layer ,Capacitor ,law ,Dissipation factor ,General Materials Science ,Composite material ,0210 nano-technology ,Electric displacement field ,High-κ dielectric - Abstract
Flexible nanocomposites composed of high dielectric constant fillers and polymer matrix have shown great potential for electrostatic capacitors and energy storage applications. To obtain the composited material with high dielectric constant and high breakdown strength, multi-interfacial composited particles, which composed of conductive cores and insulating shells and possessed the internal barrier layer capacitor (IBLC) effect, were adopted as fillers. Thus, Fe3O4@BaTiO3 core-shell particles were prepared and loaded into the poly(vinylidene fluoride-co-hexafluoropropylene) (P(VDF-HFP)) polymer matrix. As the mass fraction of core-shell fillers increased from 2.5 wt % to 30 wt %, the dielectric constant of the films increased, while the loss tangent remained at a low level (
- Published
- 2017
33. Non-invasive Prenatal Testing for Assessing Fetal Sex Chromosome Aneuploidy: A Retrospective Study of 45773 Cases
- Author
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Xinran, LU, primary, Chaohong, WANG, additional, Yuxiu, SUN, additional, Junxiang, TANG, additional, Keting, TONG, additional, and Jiansheng, ZHU, additional
- Published
- 2020
- Full Text
- View/download PDF
34. GSVM-Based Proteochemometrics Modeling for Prediction of Kinase-inhibitor Interaction
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Yan Liang, Yiqi Wang, Qingfeng Chen, and Chaohong Wang
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Control and Systems Engineering ,Chemistry ,Kinase ,Computational biology - Published
- 2016
35. Correction to Multiple Interfacial Fe
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Ling, Zhou, Qiuyun, Fu, Fei, Xue, Xiahui, Tang, Dongxiang, Zhou, Yahui, Tian, Geng, Wang, Chaohong, Wang, Haibo, Gou, and Lei, Xu
- Published
- 2018
36. Multiple Interfacial Fe
- Author
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Ling, Zhou, Qiuyun, Fu, Fei, Xue, Xiahui, Tang, Dongxiang, Zhou, Yahui, Tian, Geng, Wang, Chaohong, Wang, Haibo, Gou, and Lei, Xu
- Abstract
Flexible nanocomposites composed of high dielectric constant fillers and polymer matrix have shown great potential for electrostatic capacitors and energy storage applications. To obtain the composited material with high dielectric constant and high breakdown strength, multi-interfacial composited particles, which composed of conductive cores and insulating shells and possessed the internal barrier layer capacitor (IBLC) effect, were adopted as fillers. Thus, Fe
- Published
- 2017
37. Whole Exome Sequencing Identifies a Novel Mutation in the
- Author
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Hui, Liu, Hankui, Liu, Junxiang, Tang, Qiongfen, Lin, Yuxiu, Sun, Chaohong, Wang, Huanming, Yang, Muhammad Riaz, Khan, Mohamud Walid, Peerbux, Sohail, Ahmad, Ihtisham, Bukhari, and Jiansheng, Zhu
- Subjects
Homeodomain Proteins ,Male ,DNA Mutational Analysis ,Sequence Analysis, DNA ,Cataract ,Pedigree ,Asian People ,Mutation ,Ethnicity ,Humans ,Exome ,Family ,Female ,Child ,Transcription Factors - Abstract
Congenital cataract is the cloudiness of the eye's natural lens and is a primary cause of congenital vision loss. It accounts for almost 10% of childhood vision loss worldwide.A four generation Chinese family having seven affected individuals was recruited for the current study. Exome sequencing was performed to identify the genetic cause of congenital cataract.Analysis of data identified a novel frameshift mutation, c.608delC (p.A203fs), in theThese findings suggest that p.A203fs is the cause of cataracts in the recruited family. This information would be further helpful in the genetic diagnosis of cataract and in the genetic counseling of similar patients.
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- 2017
38. Mapping QTLs for drought tolerance in an F2:3 population from an inter-specific cross between Gossypium tomentosum and Gossypium hirsutum
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Juyun Zheng, Chaohong Wang, Xiaojing Wang, Xiaoyan Cai, G. Oluoch, Yuenan Wang, Zhongli Zhou, M. K. Riaz Khan, Kezhi Wang, Xueyuan Li, and Fang Liu
- Subjects
Genetic Markers ,0301 basic medicine ,Quantitative Trait Loci ,Drought tolerance ,Population ,Adaptation, Biological ,Biology ,Quantitative trait locus ,Chromosomes, Plant ,03 medical and health sciences ,chemistry.chemical_compound ,Dry weight ,Gene mapping ,Stress, Physiological ,Genetics ,Gossypium tomentosum ,education ,Molecular Biology ,Crosses, Genetic ,Gossypium ,education.field_of_study ,fungi ,Chromosome Mapping ,food and beverages ,General Medicine ,biology.organism_classification ,Droughts ,Horticulture ,030104 developmental biology ,chemistry ,Chlorophyll ,Microsatellite ,Microsatellite Repeats - Abstract
Cotton is one of the most important natural fiber crops in the world. Its growth and yield is greatly limited by drought. A quantitative trait locus (QTL) analysis was therefore conducted to investigate the genetic basis of drought tolerance in cotton (Gossypium spp) using 188 F2:3 lines developed from an inter-specific cross between a wild cotton species, G. tomentosum, and an upland cotton, G. hirsutum (CRI-12). A genetic map was constructed using 1295 simple sequence repeat markers, which amplified 1342 loci, distributed on 26 chromosomes, covering 3328.24 cM. A field experiment was conducted in two consecutive years (2014 and 2015) and 11 morphological and physiological traits were recorded under water-limited (W1)/well-watered (W2) regimes at three growth stages (bud, flowering, and full boll). The traits measured included chlorophyll content, plant height, leaf area, leaf number, leaf fresh weight, leaf dry weight, boll weight, number of bolls per plant, and the number of fruiting branches. Sixty-seven and 35 QTLs were found under the W1 and W2 conditions, respectively. Of these, the majority exhibited partial dominance or over-dominance genetic effects for increasing the trait values. Four consistent QTLs were found under the W1 treatment on chromosomes 5, 8, 9, and 16, whereas no consistent QTL was found in W2. Thirteen QTL clusters were also identified on nine chromosomes (2, 3, 5, 6, 9, 14, 15, 16, and 21). These results will help to elucidate the genetic basis of drought tolerance in cotton.
- Published
- 2016
39. Self-assembled core-shell CoFe2O4@BaTiO3 particles loaded P(VDF-HFP) flexible films with excellent magneto-electric effects
- Author
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Zhiping Zheng, Fei Xue, Yunxiang Hu, Xiahui Tang, Dongxiang Zhou, Wei Luo, Qiuyun Fu, Ling Zhou, Chaohong Wang, and Yahui Tian
- Subjects
Ammonium bromide ,Nanocomposite ,Materials science ,Physics and Astronomy (miscellaneous) ,Nanoparticle ,02 engineering and technology ,Dielectric ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Ferroelectricity ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Nanocrystal ,Surface modification ,Composite material ,0210 nano-technology ,Current density - Abstract
Flexible composited multiferroic films with excellent magnetic-electric effects were prepared. The films were composed of self-assembled CoFe2O4@BaTiO3 core-shell nanoparticle fillers and a poly(vinylidene fluoride-co-hexafluoropropene) matrix. The CoFe2O4@BaTiO3 core-shell particles were prepared using a hydrolysis-hydrothermal method. The surface modification of CoFe2O4 with the cationic surfactant cetyltrimethyl ammonium bromide promoted the BaTiO3 nanocrystals' self-assembly precipitation. Compared with the films loaded by bare CoFe2O4, the films filled with core-shell particles exhibited reduced leakage current density, enhanced dielectric properties, ferroelectric (FE) polarization, and magneto-electric (ME) effects. An excellent ME voltage coefficient of 1835 mV/cm Oe was measured at a DC bias field of −3500 Oe and an AC magnetic field of 3.5 Oe with a frequency of 45 kHz. This enhancement of ME effects was attributed to the large FE-ferromagnetic (FM) interface to volume and the increment of induc...
- Published
- 2017
40. Self-assembled core-shell CoFe2O4@BaTiO3 particles loaded P(VDF-HFP) flexible films with excellent magneto-electric effects.
- Author
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Ling Zhou, Qiuyun Fu, Dongxiang Zhou, Zhiping Zheng, Yunxiang Hu, Wei Luo, Yahui Tian, Chaohong Wang, Fei Xue, and Xiahui Tang
- Subjects
MULTIFERROIC materials ,FERROELECTRIC materials ,AMMONIUM bromide ,AMMONIUM compounds ,CETYLTRIMETHYLAMMONIUM bromide - Abstract
Flexible composited multiferroic films with excellent magnetic-electric effects were prepared. The films were composed of self-assembled CoFe
2 O4 @BaTiO3 core-shell nanoparticle fillers and a poly( vinylidene fluoride-co-hexafluoropropene) matrix. The CoFe2 O4 @BaTiO3 core-shell particles were prepared using a hydrolysis-hydrothermal method. The surface modification of CoFe2 O4 with the cationic surfactant cetyltrimethyl ammonium bromide promoted the BaTiO3 nanocrystals’ self-assembly precipitation. Compared with the films loaded by bare CoFe2 O4 , the films filled with core-shell particles exhibited reduced leakage current density, enhanced dielectric properties, ferroelectric (FE) polarization, and magneto-electric (ME) effects. An excellent ME voltage coefficient of 1835 mV/cm Oe was measured at a DC bias field of -3500 Oe and an AC magnetic field of 3.5 Oe with a frequency of 45 kHz. This enhancement of ME effects was attributed to the large FE-ferromagnetic (FM) interface to volume and the increment of induced polarization, which were originated from the efficient impediment of the aggregation of FM fillers in the core-shell matrix. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
41. Genetic Screening of Neonates for 20 Most Common Mutations in Deafness Associated Genes in Anhui Province of China.
- Author
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Junxiang Tang, Gang Feng, Yuxiu Sun, Chaohong Wang, Khan, Muhammad Riaz, Bukhari, Ihtisham, and Jiansheng Zhu
- Abstract
Hearing loss is the most common human genetic disorder caused by mutations in various genes. Current study was initiated to analyze the 20 most common mutations by MALDI-TOF-MS method in deafness-associated genes (GJB2, GJB3, SLC26A4 and mitochondrial 12SrRNA) in 3,331 newborns from Anhui Province of China. The analysis of data revealed that variations in GJB2 has high frequency 2.82% while SLC26A4, GJB3, and mitochondrial 12SrRNA were found to have 2.49%, 0.42%, and 0.33% respectively. This study is conducted for the first time on newborns in Anhui province, China which confirms the significant role of mutations in etiology of deafness in this population. [ABSTRACT FROM AUTHOR]
- Published
- 2016
42. The Role of Ca2+ Handling in TRIC Knockout Neonatal Cardiomyocytes
- Author
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Ki Ho Park, Chaohong Wang, Daisuke Naitou, Miyuki Nishi, Jianjie Ma, Daiju Yamazaki, Chengzhu Zhao, and Hiroshi Takeshima
- Subjects
Cardiac function curve ,Embryonic heart ,Ryanodine receptor ,Endoplasmic reticulum ,Biophysics ,Skeletal muscle ,Anatomy ,Biology ,Embryonic stem cell ,Cell biology ,medicine.anatomical_structure ,Knockout mouse ,medicine ,sense organs ,Intracellular - Abstract
Trimeric intracellular cation (TRIC) channel subtypes, namely TRIC-A and TRIC-B, are essential counter-ion permeability pathway associated with rapid Ca2+ release from intracellular stores. TRIC-A abundantly expressed in excitable tissues including brain, heart, smooth muscle and skeletal muscle, whereas TRIC-B is ubiquitously expressed throughout tissues. Though TRIC-A-knockout mice are normal in terms of growth, the mice developed hypertension even at young-adult stage. On the other hand, TRIC-B-knockout mice exhibit neonatal lethality due to respiratory failure. Moreover, knockout mice lacking both TRIC channel subtypes exhibit embryonic heart failure, and the mutant cardiomyocytes show compromised ryanodine receptor (RyR)-mediated Ca2+ release. These evidences are indicating that TRIC channel subtypes possess compatible functions in embryonic cardiomyocytes.Because of neonatal lethality occurs in TRIC-B-knockout mice, we examined Ca2+ handling properties in wild-type, TRIC-A-knockout and TRIC-B-knockout neonatal cardiomyocytes. Resting Ca2+ level and Ca2+ transient frequency under isoproterenol treatment were enhanced in TRIC-B-kncokout cardiomyocytes, and caffeine responses under Ca2+-free condition was amplified in both knockout cardiomyocytes.Therefore, TRIC channels are likely to act coordinately on synchronization with Ca2+ release from sarcoplasmic reticulum to maintain normal cardiac function. Furthermore, TRIC-A-knockout mice showed arrhythmia under acutely isoproterenol injection and reduction of Ca2+ spark frequency. Resting Ca2+ level and Ca2+ transient amplitude were augmented in adult TRIC-A-knockout cardiomyocytes. These results seem to support this study.
- Published
- 2013
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