36 results on '"Chang-Ning Li"'
Search Results
2. Physiological and molecular insights into the resilience of biological nitrogen fixation to applied nitrogen in Saccharum spontaneum, wild progenitor of sugarcane
- Author
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Ting Luo, Chang-Ning Li, Rui Yan, Kejun Huang, Yang-Rui Li, Xiao-Yan Liu, and Prakash Lakshmanan
- Subjects
hormone ,nitrogen ,fixation ,Saccharum spontaneum ,sugarcane ,Plant culture ,SB1-1110 - Abstract
Excessive use of nitrogen (N) fertilizer for sugarcane cultivation is a significant cause of greenhouse gas emission. N use-efficiency (NUE) of sugarcane is relatively low, and considerable effort is now directed to exploit biological nitrogen fixation (BNF) in sugarcane. We hypothesize that genetic base-broadening of sugarcane using high-BNF Saccharum spontaneum, a wild progenitor of sugarcane, will help develop N-efficient varieties. We found remarkable genetic variation for BNF and growth in S. spontaneum accessions, and BNF in some accessions remained highly resilient to inorganic N application. Physiological and molecular analyses of two S. spontaneum accessions with high-BNF capacity and growth, namely G152 and G3, grown under N replete and low N conditions showed considerable similarity for total N, NH4-N, soluble sugar, indoleacetic acid, gibberellic acid, zeatin and abscisic acid content; yet, they were strikingly different at molecular level. Global gene expression analysis of G152 and G3 grown under contrasting N supply showed genotype effect explaining much of the gene expression variation observed. Differential gene expression analysis found an over-representation of carbohydrate and amino acid metabolism and transmembrane transport genes in G152 and an enrichment of lipid metabolism and single-organism processes genes in G3, suggesting that distinctly divergent metabolic strategies are driving N-related processes in these accessions. This was attested by the remarkable variation in carbon, N, amino acid and hormone metabolism-related gene expression in G152 and G3 under high- and low-N supply. We conclude that both accessions may be achieving similar BNF and growth phenotypes through overlapping but distinctly different biochemical and molecular mechanisms.
- Published
- 2023
- Full Text
- View/download PDF
3. Exploration of silicon functions to integrate with biotic stress tolerance and crop improvement
- Author
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Xiu-Peng Song, Krishan K. Verma, Dan-Dan Tian, Xiao-Qiu Zhang, Yong-Jian Liang, Xing Huang, Chang-Ning Li, and Yang-Rui Li
- Subjects
Antioxidants ,Biotic stress ,Crop improvement ,Physiology ,Sustainable agriculture ,Silicon ,Biology (General) ,QH301-705.5 - Abstract
Abstract In the era of climate change, due to increased incidences of a wide range of various environmental stresses, especially biotic and abiotic stresses around the globe, the performance of plants can be affected by these stresses. After oxygen, silicon (Si) is the second most abundant element in the earth’s crust. It is not considered as an important element, but can be thought of as a multi-beneficial quasi-essential element for plants. This review on silicon presents an overview of the versatile role of this element in a variety of plants. Plants absorb silicon through roots from the rhizospheric soil in the form of silicic or monosilicic acid. Silicon plays a key metabolic function in living organisms due to its relative abundance in the atmosphere. Plants with higher content of silicon in shoot or root are very few prone to attack by pests, and exhibit increased stress resistance. However, the more remarkable impact of silicon is the decrease in the number of seed intensities/soil-borne and foliar diseases of major plant varieties that are infected by biotrophic, hemi-biotrophic and necrotrophic pathogens. The amelioration in disease symptoms are due to the effect of silicon on a some factors involved in providing host resistance namely, duration of incubation, size, shape and number of lesions. The formation of a mechanical barrier beneath the cuticle and in the cell walls by the polymerization of silicon was first proposed as to how this element decreases plant disease severity. The current understanding of how this element enhances resistance in plants subjected to biotic stress, the exact functions and mechanisms by which it modulates plant biology by potentiating the host defence mechanism needs to be studied using genomics, metabolomics and proteomics. The role of silicon in helping the plants in adaption to biotic stress has been discussed which will help to plan in a systematic way the development of more sustainable agriculture for food security and safety in the future.
- Published
- 2021
- Full Text
- View/download PDF
4. High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (Saccharum spp. Inter-Specific Hybrids)
- Author
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Mukesh Kumar Malviya, Chang-Ning Li, Prakash Lakshmanan, Manoj Kumar Solanki, Zhen Wang, Anjali Chandrol Solanki, Qian Nong, Krishan K. Verma, Rajesh Kumar Singh, Pratiksha Singh, Anjney Sharma, Dao-Jun Guo, Eldessoky S. Dessoky, Xiu-Peng Song, and Yang-Rui Li
- Subjects
rhizosphere soil ,sugarcane ,nifH ,16S rRNA ,diazotroph diversity ,Plant culture ,SB1-1110 - Abstract
Considering the significant role of genetic background in plant-microbe interactions and that most crop rhizospheric microbial research was focused on cultivars, understanding the diversity of root-associated microbiomes in wild progenitors and closely related crossable species may help to breed better cultivars. This study is aimed to fill a critical knowledge gap on rhizosphere and diazotroph bacterial diversity in the wild progenitors of sugarcane, the essential sugar and the second largest bioenergy crop globally. Using a high-throughput sequencing (HTS) platform, we studied the rhizosphere and diazotroph bacterial community of Saccharum officinarum L. cv. Badila (BRS), Saccharum barberi (S. barberi) Jesw. cv Pansahi (PRS), Saccharum robustum [S. robustum; (RRS), Saccharum spontaneum (S. spontaneum); SRS], and Saccharum sinense (S. sinense) Roxb. cv Uba (URS) by sequencing their 16S rRNA and nifH genes. HTS results revealed that a total of 6,202 bacteria-specific operational taxonomic units (OTUs) were identified, that were distributed as 107 bacterial groups. Out of that, 31 rhizobacterial families are commonly spread in all five species. With respect to nifH gene, S. barberi and S. spontaneum recorded the highest and lowest number of OTUs, respectively. These results were validated by quantitative PCR analysis of both genes. A total of 1,099 OTUs were identified for diazotrophs with a core microbiome of 9 families distributed among all the sugarcane species. The core microbiomes were spread across 20 genera. The increased microbial diversity in the rhizosphere was mainly due to soil physiochemical properties. Most of the genera of rhizobacteria and diazotrophs showed a positive correlation, and few genera negatively correlated with the soil properties. The results showed that sizeable rhizospheric diversity exists across progenitors and close relatives. Still, incidentally, the rhizosphere microbial abundance of progenitors of modern sugarcane was at the lower end of the spectrum, indicating the prospect of Saccharum species introgression breeding may further improve nutrient use and disease and stress tolerance of commercial sugarcane. The considerable variation for rhizosphere microbiome seen in Saccharum species also provides a knowledge base and an experimental system for studying the evolution of rhizobacteria-host plant association during crop domestication.
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- 2022
- Full Text
- View/download PDF
5. Sugarcane-Legume Intercropping Can Enrich the Soil Microbiome and Plant Growth
- Author
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Mukesh Kumar Malviya, Manoj Kumar Solanki, Chang-Ning Li, Zhen Wang, Yuan Zeng, Krishan K. Verma, Rajesh Kumar Singh, Pratiksha Singh, Hai-Rong Huang, Li-Tao Yang, Xiu-Peng Song, and Yang-Rui Li
- Subjects
microbial diversity ,sugarcane rhizobiome ,soil properties ,plant growth ,high-throughput sequencing ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Soil microbes have a direct impact on plant metabolism and health. The current study investigates the comparative rhizobiome between sugarcane monoculture and sugarcane–soybean intercropping. A greenhouse experiment was performed with two treatments: (1) sugarcane monoculture and (2) sugarcane–soybean intercropped. We used a high-throughput sequencing (HTS) platform to analyze the microbial community. We used the 16S rRNA gene and internal transcribed spacer region primers to identify the microbial diversity. HTS results revealed that a total of 2,979 and 124 bacterial and fungal operational taxonomic units (OTUs) were observed, respectively. Microbial diversity results concluded that the intercropping system has a beneficial impact on soil microbes. The highest numbers of bacterial and fungal OTUs were found in the intercropping system, and these results also collaborated with quantitative PCR results. Additionally, intercropped sugarcane plants showed a higher weight of above- and below-ground parts than the monoculture. Soil chemical analysis results also complemented that the intercropping system nourished organic carbon, total nitrogen, and soil enzyme activities. Correlation analysis of the diversity index and abundance concluded that soil nutrient content positively influenced the microbial abundance that improves plant growth. The present study frames out the profound insights of microbial community interaction under the sugarcane–soybean intercropping system. This information could help improve or increase the sugarcane crop production without causing any negative impact on sugarcane plant growth and development.
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- 2021
- Full Text
- View/download PDF
6. Assessment of Diazotrophic Proteobacteria in Sugarcane Rhizosphere When Intercropped With Legumes (Peanut and Soybean) in the Field
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Manoj Kumar Solanki, Zhen Wang, Fei-Yong Wang, Chang-Ning Li, Chhedi Lal Gupta, Rajesh Kumar Singh, Mukesh Kumar Malviya, Pratiksha Singh, Li-Tao Yang, and Yang-Rui Li
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intercropping ,microbial community ,high throughput sequencing ,NifH gene ,sugarcane ,Microbiology ,QR1-502 - Abstract
Several factors influenced the sugarcane production, and among them, higher use of nitrogen and depletion of soil nutrient constitutes a significant concern in China. Sugarcane-legume intercropping may help to regulate the microbial structure and functions. In the present study, sugarcane rhizosphere soils of three cropping systems: Sugarcane only (S-only), sugarcane with peanut (S + P), and sugarcane + soybean (S + S) were sampled in tillering, elongation, and maturation stages from two different experimental fields. High-throughput sequencing technologies applied to assess the effects of different cropping systems on the structure of nitrogenase (nifH) gene communities. A total of 3818 OTUs (operational taxonomic units) were acquired from all soil samples. Intercropping systems noticeably increased the relative abundance of Proteobacteria in the tillering stage. The increased microbial diversity in the rhizosphere was mainly due to soil organic carbon and total soil N. In contrast, intercropping has no significant negative impact on microbial abundance, but sugarcane growth stages influence it significantly, and two bacteria (Bradyrhizobium and Pseudacidovorax) showed significant shift during plant growth. The results provide insight into the microbial structure of Proteobacteria in the sugarcane legume-intercropping field, and how microbial community behaves in different growth stages. It can boost the microbial activity of the soil, and that could be a new strategy to stimulate soil fertility without causing any negative impact on crop production.
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- 2020
- Full Text
- View/download PDF
7. Comparative analysis of sugarcane root transcriptome in response to the plant growth-promoting Burkholderia anthina MYSP113.
- Author
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Mukesh Kumar Malviya, Chang-Ning Li, Manoj Kumar Solanki, Rajesh Kumar Singh, Reemon Htun, Pratiksha Singh, Krishan K Verma, Li-Tao Yang, and Yang-Rui Li
- Subjects
Medicine ,Science - Abstract
The diazotrophic Burkholderia anthina MYSP113 is a vital plant growth-promoting bacteria and sugarcane root association. The present study based on a detailed analysis of sugarcane root transcriptome by using the HiSeq-Illumina platform in response to the strain MYSP113. The bacterium was initially isolated from the rhizosphere of sugarcane. To better understand biological, cellular, and molecular mechanisms, a de novo transcriptomic assembly of sugarcane root was performed. HiSeq-Illumina platformwas employed for the sequencing of an overall of 16 libraries at a 2×100 bp configuration. Differentially expressed genes (DEGs) analysis identified altered gene expression in 370 genes (total of 199 up-regulated genes and 171 down-regulated genes). Deciphering the huge datasets, concerning the functioning and production of biological systems, a high throughput genome sequencing analysis was attempted with Gene ontology functional analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The report revealed a total of 148930 unigenes. 70414 (47.28%) of them were annotated successfully to Gene Ontology (GO) terms. 774 at 45 days, 4985 of 30 days and 15 days of 6846 terms were significantly regulated. GO analysis revealed that many genes involved in the metabolic, oxidation-reduction process and biological regulatory processes in response to strain MYSP113 and significantly enriched as compare to the control. Moreover, KEGG enriched results show that differentially expressed genes were classified into different pathway categories involved in various processes, such as nitrogen metabolism, plant hormone signal transduction, etc. The sample correlation analyses could help examine the similarity at the gene expression level. The reliability of the observed differential gene expression patterns was validated with quantitative real-time PCR (qRT-PCR). Additionally, plant enzymes activities such as peroxidase and superoxide dismutase were significantly increased in plant roots after the inoculation of strain MYSP113. The results of the research may help in understanding the plant growth-promoting rhizobacteria and plant interaction.
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- 2020
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8. Effects of Autotoxicity on Alfalfa (Medicago sativa): Seed Germination, Oxidative Damage and Lipid Peroxidation of Seedlings
- Author
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Xiao-Yan Zhang, Shang-Li Shi, Xiao-Long Li, Chang-Ning Li, Cui-Mei Zhang, Yun A, Wen-Juan Kang, and Guo-Li Yin
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alfalfa ,autotoxin ,germplasm ,abiotic stress ,autotoxicity effect ,Agriculture - Abstract
Alfalfa (Medicago sativa) is a highly valuable perennial forage legume that suffers from autotoxicity, which decreases plant resistance, reduces soil fertility, causes serious soil-borne diseases, and promotes ecological imbalance. We evaluated the effects of autotoxicity on the seed germination of 22 alfalfa varieties, and then elucidated the oxidative damage and lipid peroxidation in two alfalfa varieties with contrasting autotoxicity tolerances. The technique for order of preference by similarity to ideal solution (TOPSIS) method was used to rank the germination of the 22 alfalfa varieties when exposed to six autotoxic concentrations (0, 0.025, 0.075, 0.125, 0.175, and 0.225 g∙mL−1). We found WL656HQ and 3105C to be autotoxicity-tolerant and autotoxicity-sensitive varieties, respectively. The germination index mainly affects the comprehensive allelopathic index of WL656HQ and 3105C, which were the simple vigor index and radicle length according to the random forest model, respectively. 3105C eliminates reactive oxygen species (ROS) via antioxidant enzymes and antioxidants under T1 (0.025 g∙mL−1), but the oxidative stress system and the oxidative scavenging system cannot maintain the balance under T2 (0.125 g∙mL−1), causing oxidative bursts. In comparison, WL656HQ used its oxidative scavenging system (peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR)) to maintain its redox dynamic balance by removing excess ROS at all concentrations. In conclusion, the positive and negative indicators of autotoxicity for the two varieties were ascorbate (ASA) and hydroxyl free radicals (OH•), and proline (Pro) and dehydroascrobate (DHA), respectively. The most sensitive autotoxic concentrations of 3105C and WL656HQ were T2 (0.125 g∙mL−1) and T1 (0.025 g∙mL−1), respectively.
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- 2021
- Full Text
- View/download PDF
9. Research Progress of Ceramic and Metal Welding Technology
- Author
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Chang Ning LI and Shao Hua Yin
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics - Abstract
The connection characteristics and methods of ceramic and metal were studied, and emphatically the current research status of ceramic and metal active brazing, diffusion welding, self-propagating high temperature synthesis welding, fusion welding, friction welding, ultrasonic welding, hot-press reaction sintering welding and other welding methods were introduced. The main problems and improvement methods existing in the current ceramic and metal welding are discussed, and the development trend of ceramic and metal brazing connection is prospected.
- Published
- 2023
10. High-Throughput Sequencing-Based Analysis of Rhizosphere and Diazotrophic Bacterial Diversity Among Wild Progenitor and Closely Related Species of Sugarcane (
- Author
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Mukesh Kumar, Malviya, Chang-Ning, Li, Prakash, Lakshmanan, Manoj Kumar, Solanki, Zhen, Wang, Anjali Chandrol, Solanki, Qian, Nong, Krishan K, Verma, Rajesh Kumar, Singh, Pratiksha, Singh, Anjney, Sharma, Dao-Jun, Guo, Eldessoky S, Dessoky, Xiu-Peng, Song, and Yang-Rui, Li
- Abstract
Considering the significant role of genetic background in plant-microbe interactions and that most crop rhizospheric microbial research was focused on cultivars, understanding the diversity of root-associated microbiomes in wild progenitors and closely related crossable species may help to breed better cultivars. This study is aimed to fill a critical knowledge gap on rhizosphere and diazotroph bacterial diversity in the wild progenitors of sugarcane, the essential sugar and the second largest bioenergy crop globally. Using a high-throughput sequencing (HTS) platform, we studied the rhizosphere and diazotroph bacterial community of
- Published
- 2021
11. Effects of Autotoxicity on Alfalfa (Medicago sativa): Seed Germination, Oxidative Damage and Lipid Peroxidation of Seedlings
- Author
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Yun A, Zhang Cuimei, Shangli Shi, Xiao-Long Li, Chang-Ning Li, Wen-Juan Kang, Yin Guoli, and Xiao-Yan Zhang
- Subjects
0106 biological sciences ,0301 basic medicine ,Antioxidant ,abiotic stress ,medicine.medical_treatment ,Glutathione reductase ,Autotoxicity ,medicine.disease_cause ,01 natural sciences ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,autotoxin ,Radicle ,medicine ,autotoxicity effect ,Chemistry ,food and beverages ,Agriculture ,germplasm ,APX ,Horticulture ,030104 developmental biology ,Germination ,Agronomy and Crop Science ,alfalfa ,Oxidative stress ,010606 plant biology & botany - Abstract
Alfalfa (Medicago sativa) is a highly valuable perennial forage legume that suffers from autotoxicity, which decreases plant resistance, reduces soil fertility, causes serious soil-borne diseases, and promotes ecological imbalance. We evaluated the effects of autotoxicity on the seed germination of 22 alfalfa varieties, and then elucidated the oxidative damage and lipid peroxidation in two alfalfa varieties with contrasting autotoxicity tolerances. The technique for order of preference by similarity to ideal solution (TOPSIS) method was used to rank the germination of the 22 alfalfa varieties when exposed to six autotoxic concentrations (0, 0.025, 0.075, 0.125, 0.175, and 0.225 g∙mL−1). We found WL656HQ and 3105C to be autotoxicity-tolerant and autotoxicity-sensitive varieties, respectively. The germination index mainly affects the comprehensive allelopathic index of WL656HQ and 3105C, which were the simple vigor index and radicle length according to the random forest model, respectively. 3105C eliminates reactive oxygen species (ROS) via antioxidant enzymes and antioxidants under T1 (0.025 g∙mL−1), but the oxidative stress system and the oxidative scavenging system cannot maintain the balance under T2 (0.125 g∙mL−1), causing oxidative bursts. In comparison, WL656HQ used its oxidative scavenging system (peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR)) to maintain its redox dynamic balance by removing excess ROS at all concentrations. In conclusion, the positive and negative indicators of autotoxicity for the two varieties were ascorbate (ASA) and hydroxyl free radicals (OH•), and proline (Pro) and dehydroascrobate (DHA), respectively. The most sensitive autotoxic concentrations of 3105C and WL656HQ were T2 (0.125 g∙mL−1) and T1 (0.025 g∙mL−1), respectively.
- Published
- 2021
- Full Text
- View/download PDF
12. Comparative transcriptome profiling of resistant and susceptible sugarcane genotypes in response to the airborne pathogen Fusarium verticillioides
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Yijing Gao, Liang Qiang, Wang Zeping, Li Yijie, Lei Jingchao, Xiupeng Song, and Chang-Ning Li
- Subjects
0301 basic medicine ,Genotype ,genetic structures ,medicine.medical_treatment ,Biology ,Transcriptome ,03 medical and health sciences ,0302 clinical medicine ,Fusarium ,Gene Expression Regulation, Plant ,Genetics ,medicine ,RNA-Seq ,KEGG ,Molecular Biology ,Gene ,Disease Resistance ,Plant Diseases ,chemistry.chemical_classification ,Protease ,Phenylpropanoid ,Gene Expression Profiling ,General Medicine ,Protein Metabolism Process ,Saccharum ,Metabolic pathway ,030104 developmental biology ,Enzyme ,chemistry ,Biochemistry ,030220 oncology & carcinogenesis ,Host-Pathogen Interactions - Abstract
Fusarium verticillioides is the pathogen associated with pokkah boeng disease (PBD), the most significant airborne disease of sugarcane. The molecular mechanisms that regulate the defense responses of sugarcane towards this fungus are not yet fully known. Samples of 'YT 94/128' (resistant, R) and 'GT 37' (susceptible, S) inoculated with F. verticillioides on the 14 days post-inoculation were used to analyze the transcriptome to screen R genes. In total, 80.93 Gb of data and 76,175 Unigenes were obtained after assembling the sequencing data, and comparisons of Unigenes with NR, Swiss-prot, KOG, and KEGG databases confirmed 42,451 Unigenes. The analysis of differentially expression genes (DEGs) in each sample revealed 9092 DEGs in 'YT 94/128,' including 8131 up-regulated DEGs and 961 down-regulated DEGs; there were 9829 DEGs in 'GT 37,' including 7552 up-regulated DEGs and 2277 down-regulated DEGs. The identified DEGs were mainly involved in catalytic enzyme activity, cell protease, hydrolytic enzymes, peptide enzyme, protein metabolism process of negative regulation, phenylpropanoid metabolism, extracellular region, aldehyde dehydrogenase, endopeptidase, REDOX enzyme, protein kinases, and phosphoric acid transferase categories. KEGG pathway clustering analysis showed that the DEGs involved in resistance were significantly related to metabolic pathways of phenylpropanoid biosynthesis, cutin, suberine and wax biosynthesis, nitrogenous metabolism, biosynthesis of secondary metabolites, and plant-pathogen interactions. This application of transcriptomic data clarifies the mechanism of interactions between sugarcane and F. verticillioides, which can help to reveal disease-related metabolic pathways, molecular regulatory networks, and key genes involved in sugarcane responses to F. verticillioides.
- Published
- 2019
13. Beneficial Linkages of Endophytic Burkholderia anthina MYSP113 Towards Sugarcane Growth Promotion
- Author
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Pratiksha Singh, Rajesh Singh, Li-Tao Yang, Yang-Rui Li, Mukesh Kumar Malviya, Reemon Htun, Manoj Kumar Solanki, and Chang-Ning Li
- Subjects
0106 biological sciences ,Rhizosphere ,biology ,Inoculation ,fungi ,food and beverages ,04 agricultural and veterinary sciences ,biology.organism_classification ,01 natural sciences ,chemistry.chemical_compound ,Horticulture ,Burkholderia ,chemistry ,Dry weight ,Chitinase ,040103 agronomy & agriculture ,biology.protein ,0401 agriculture, forestry, and fisheries ,Burkholderia anthina ,Agronomy and Crop Science ,Abscisic acid ,Bacteria ,010606 plant biology & botany - Abstract
Microbial agents are the best alternative to the chemical fertilizers that promote the plant growth in an eco-friendly manner. This study was intended to characterize Burkholderia anthina strain MYSP113 and to evaluate its mechanisms linked to sugarcane growth promotion. The morphological, biochemical, and molecular characterization (gyrB and recA) results identified the bacteria as B. anthina. To determine the plant bacterial association, MYSP113 strain was tagged with a plasmid-bear green fluorescent protein (gfp) gene, and the tagged strain was inoculated to the sugarcane (variety GXB-9), and bacterial colonization analyzed by confocal laser fluorescence microscopy. The results revealed that strain MYSP113 had root-colonizing abilities, and colonization was detected at the root and leaves maturation zones. Bacteria entered the roots via cracks at the surfacing site of the roots and disrupted epidermis. Bacterial cells were observed in the substomatal chambers of leaves. Furthermore, to evaluate the mechanisms related to sugarcane growth promotion and bacterial competence in soil and plant, greenhouse experiment performed, and bacterial competence, plant growth parameters, and phytoenzymes, and hormones were assessed with or without MYSP113. We examined bacterial competence in soil and plant via qPCR and direct plant count at 45 and 90 DAI, and results recommended that strain MYSP113 has the ability to colonize in sugarcane rhizosphere and in the aerial part of the plant. Furthermore, strain MYSP113 significantly induced the plant fresh weight, dry weight, and chlorophyll content of sugarcane seedlings at 45 and 90 days after inoculation. Additionally, strain MYSP113 triggered chitinase, cellulose, indole acetic acid, and abscisic acid production in the plant roots and aerial parts, and these results signifying that bacterial inoculation modulates the plant metabolism and boost the health. The present study concluded that strain MYSP113 has beneficial linkages with the sugarcane plant, and it would be utilized as a bioinoculant in future.
- Published
- 2019
14. Rhizospheric and endospheric diazotrophs mediated soil fertility intensification in sugarcane-legume intercropping systems
- Author
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Li-Tao Yang, Tao-Ju Lan, Yang-Rui Li, Fei-Yong Wang, Manoj Kumar Solanki, Chang-Ning Li, Zhen Wang, Pratiksha Singh, and Rajesh Kumar Singh
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Rhizosphere ,education.field_of_study ,biology ,Stratigraphy ,Soil organic matter ,Population ,food and beverages ,Intercropping ,04 agricultural and veterinary sciences ,010501 environmental sciences ,biology.organism_classification ,01 natural sciences ,Agronomy ,040103 agronomy & agriculture ,Nitrogen fixation ,0401 agriculture, forestry, and fisheries ,Diazotroph ,Soil fertility ,Monoculture ,education ,0105 earth and related environmental sciences ,Earth-Surface Processes - Abstract
Sugarcane-legume intercrops have gained attention because of their ability to improve crop productivity in an ecofriendly manner. We investigated the roles of rhizospheric and endospheric diazotrophs in soil nutrient transformation. We conducted two field experiments with three treatments: sugarcane monoculture, sugarcane + peanut intercrop, and sugarcane + soybean intercrop. Soil and plant samples were collected and the nutrient content, enzymatic activity, and diazotroph population were assessed. The nitrogenase (nifH) gene and 16S rRNA gene were used for molecular characterization. Correlation analysis was performed to discover the relations among the soil variables, plant variables, and microbial communities. The multiple variance analysis results indicated that the intercropping systems had a significant (p
- Published
- 2018
15. Exploration of silicon functions to integrate with biotic stress tolerance and crop improvement
- Author
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Chang-Ning Li, Dan-Dan Tian, Xiaoqiu Zhang, Xiu-Peng Song, Krishan K. Verma, Yang-Rui Li, Xing Huang, and Yong-Jian Liang
- Subjects
0106 biological sciences ,0301 basic medicine ,Silicon ,QH301-705.5 ,Physiology ,chemistry.chemical_element ,Review ,Crop improvement ,Biology ,01 natural sciences ,Antioxidants ,03 medical and health sciences ,Soil ,Metabolomics ,Biotic stress ,Stress, Physiological ,Biology (General) ,Abiotic component ,Resistance (ecology) ,fungi ,technology, industry, and agriculture ,Sustainable agriculture ,food and beverages ,Agriculture ,Plants ,Plant disease ,030104 developmental biology ,Agronomy ,chemistry ,Shoot ,Developmental biology ,010606 plant biology & botany - Abstract
In the era of climate change, due to increased incidences of a wide range of various environmental stresses, especially biotic and abiotic stresses around the globe, the performance of plants can be affected by these stresses. After oxygen, silicon (Si) is the second most abundant element in the earth’s crust. It is not considered as an important element, but can be thought of as a multi-beneficial quasi-essential element for plants. This review on silicon presents an overview of the versatile role of this element in a variety of plants. Plants absorb silicon through roots from the rhizospheric soil in the form of silicic or monosilicic acid. Silicon plays a key metabolic function in living organisms due to its relative abundance in the atmosphere. Plants with higher content of silicon in shoot or root are very few prone to attack by pests, and exhibit increased stress resistance. However, the more remarkable impact of silicon is the decrease in the number of seed intensities/soil-borne and foliar diseases of major plant varieties that are infected by biotrophic, hemi-biotrophic and necrotrophic pathogens. The amelioration in disease symptoms are due to the effect of silicon on a some factors involved in providing host resistance namely, duration of incubation, size, shape and number of lesions. The formation of a mechanical barrier beneath the cuticle and in the cell walls by the polymerization of silicon was first proposed as to how this element decreases plant disease severity. The current understanding of how this element enhances resistance in plants subjected to biotic stress, the exact functions and mechanisms by which it modulates plant biology by potentiating the host defence mechanism needs to be studied using genomics, metabolomics and proteomics. The role of silicon in helping the plants in adaption to biotic stress has been discussed which will help to plan in a systematic way the development of more sustainable agriculture for food security and safety in the future.
- Published
- 2021
16. Sugarcane microbiome: role in sustainable production
- Author
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Mukesh Kumar Malviya, Chang-Ning Li, Reemon Htun, Yang-Rui Li, Manoj Kumar Solanki, Pratiksha Singh, and Rajesh Kumar Singh
- Subjects
Crop ,Agriculture ,business.industry ,Biofuel ,Biofertilizer ,Sustainability ,Microbiome ,Biology ,Agricultural productivity ,business ,Bagasse ,Biotechnology - Abstract
Plants are associated with the combination of microbiomes, which can improve plant growth, stress tolerance, and control plant pathogens. The incorporation of a beneficial microbiome represents a promising sustainable key to enhance agricultural production. Sugarcane is an essential crop with many agricultural and industrial uses including animal feed, biofuels and bagasse. There is a need to strengthen sugarcane crop production sustainably and to locate solutions to control pathogens, abiotic stress, and pests. The utilization of beneficial microbes as a biofertilizer has become essential in sugarcane agriculture for sustainable crop production. In this perspective, the sugarcane microbiome becomes a promising approach for sustainable production. The chapter describes the application of sugarcane-associated microbiome.
- Published
- 2021
17. List of Contributors
- Author
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S.K. Aggarwal, Ajit Kumar Dubedi Anal, Rizwan Ali Ansari, Samadhan Yuvraj Bagul, Siddhartha Sankar Biswas, Uma Chaurasiya, Jeet Ram Choudhary, Mukesh Choudhary, Balasubramanian Cibichakravarthy, Murugesh Easwaran, Reemon Htun, Touseef Hussain, P. Jishma, Silju Juby, Nanjappan Karthikeyan, Brijendra Kumar Kashyap, Prem Lal Kashyap, Shuchi Kaushik, Jae-Yean Kim, Ashutosh Kumar, Hosur Hanumegowda Kumaraswamy, Baby Kumari, Prity Kushwaha, Chang-Ning Li, Yang-Rui Li, Irshad Mahmood, M.A. Mallick, Mukesh Kumar Malviya, Deepak Kumar Maurya, Sahil Mehta, Raghvendra Kumar Mishra, Puja Pandey, Kuppusamy Pandiyan, Priya Bharti Patel, Anupam Patra, Solai Ramatchandirane Prabagaran, Dibyajyoti Pramanik, Selvakumar Princy, E.K. Radhakrishnan, Shalini Rai, Rose Rizvi, Trisha Roy, Madhumonti Saha, Abhijit Sarkar, Anil Kumar Saxena, Abhishek Sharma, Neha Sharma, Shikha Sharma, Rahul Mahadev Shelake, Neelabh Shrivastava, Baljinder Singh, Indranil Singh, Pratiksha Singh, Rajesh Kumar Singh, Sanjay K. Singh, Anjali Chandrol Solanki, Manoj Kumar Solanki, Aisha Sumbul, Harish Suthar, R.S. Tomar, Anurag Tripathi, Mala Trivedi, Swati Upadhyay, Rajesh Ramdas Waghunde, and Kaushlesh K. Yadav
- Published
- 2021
18. Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions
- Author
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Mo Zhanghong, Manoj Kumar Solanki, Mukesh Kumar Malviya, Zhen Wang, Xing Huang, Xie Jinlan, Yang-Rui Li, Nong Qian, Xiu-Peng Song, Lin Li, and Chang-Ning Li
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0106 biological sciences ,0301 basic medicine ,Science ,Drought tolerance ,Plant physiology ,Biology ,01 natural sciences ,Article ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Gene ,Genetics ,Abiotic component ,Multidisciplinary ,Drought ,Dehydration ,Gene Expression Profiling ,Saccharum spontaneum ,fungi ,RNA ,food and beverages ,biology.organism_classification ,mRNA surveillance ,Saccharum ,Plant Leaves ,030104 developmental biology ,Medicine ,Plant hormone ,010606 plant biology & botany - Abstract
As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum. Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.
- Published
- 2020
19. Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.)
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Manoj Kumar Solanki, Zeping Wang, Chang-Ning Li, Jiguang Wei, Li Yijie, Reemon Htun, Xie Jinlan, Qiang Liang, Nong Qian, Liu Xiaoyan, Mukesh Kumar Malviya, and Yang-Rui Li
- Subjects
0106 biological sciences ,0301 basic medicine ,Arabidopsis ,lcsh:Medicine ,Sequence alignment ,Protein Serine-Threonine Kinases ,01 natural sciences ,Article ,03 medical and health sciences ,Phylogenetics ,Gene Expression Regulation, Plant ,Stress, Physiological ,Gene family ,lcsh:Science ,Gene ,Conserved Sequence ,Phylogeny ,Plant Proteins ,Genetics ,Regulation of gene expression ,Multidisciplinary ,biology ,Phylogenetic tree ,Arabidopsis Proteins ,lcsh:R ,Computational Biology ,biology.organism_classification ,Droughts ,Saccharum ,030104 developmental biology ,Structural Homology, Protein ,lcsh:Q ,Sequence Alignment ,Functional divergence ,010606 plant biology & botany ,Abscisic Acid ,Signal Transduction - Abstract
In plants, both abscisic acid (ABA) dependent and independent pathways form the basis for the response to environmental stresses. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) plays a central role in plant stress signal transduction. However, complete annotation and specific expression patterns of SnRK2s in sugarcane remain unclear. For the present study, we performed a full-length cDNA library survey of sugarcane, thus identifying ten SoSnRK2 genes via phylogenetic, local BLAST methods, and various bioinformatics analyses. Phylogenetic analysis indicated division of SoSnRK2 genes into three subgroups, similar to other plant species. Gene structure comparison with Arabidopsis suggested a unique evolutionary imprint of the SnRK2 gene family in sugarcane. Both sequence alignment and structural annotation provided an overview of the conserved N-terminal and variations of the C-terminal, suggesting functional divergence. Transcript and transient expression assays revealed SoSnRK2s to be involved in the responses to diverse stress signals, and strong ABA induction of SoSnRK2s in subgroup III. Co-expression network analyses indicated the existence of both conserved and variable biological functions among different SoSnRK2s members. In summary, this comprehensive analysis will facilitate further studies of the SoSnRK2 family and provide useful information for the functional validation of SoSnRK2s.
- Published
- 2017
20. Digitalization of toxicology: improving preclinical to clinical translation
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Brian R. Berridge, Szczepan W. Baran, Vivek Kumar, Natalie Bratcher-Petersen, Michael Ellis, Chang-Ning Liu, and Timothy L. Robertson
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toxicology ,artificial intelligence ,machine learning ,digital ,biomarker ,Toxicology. Poisons ,RA1190-1270 - Abstract
Though the portfolio of medicines that are extending and improving the lives of patients continues to grow, drug discovery and development remains a challenging business on its best day. Safety liabilities are a significant contributor to development attrition where the costliest liabilities to both drug developers and patients emerge in late development or post-marketing. Animal studies are an important and influential contributor to the current drug discovery and development paradigm intending to provide evidence that a novel drug candidate can be used safely and effectively in human volunteers and patients. However, translational gaps—such as toxicity in patients not predicted by animal studies—have prompted efforts to improve their effectiveness, especially in safety assessment. More holistic monitoring and “digitalization” of animal studies has the potential to enrich study outcomes leading to datasets that are more computationally accessible, translationally relevant, replicable, and technically efficient. Continuous monitoring of animal behavior and physiology enables longitudinal assessment of drug effects, detection of effects during the animal’s sleep and wake cycles and the opportunity to detect health or welfare events earlier. Automated measures can also mitigate human biases and reduce subjectivity. Reinventing a conservative, standardized, and traditional paradigm like drug safety assessment requires the collaboration and contributions of a broad and multi-disciplinary stakeholder group. In this perspective, we review the current state of the field and discuss opportunities to improve current approaches by more fully leveraging the power of sensor technologies, artificial intelligence (AI), and animal behavior in a home cage environment.
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- 2024
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21. Sugar Industry and Improved Sugarcane Farming Technologies in China
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Hongwei Tan, Qiang Liang, Xiu-Peng Song, Li-Tao Yang, Chang-Ning Li, Wang Weizan, Yang-Rui Li, Jian-Ming Wu, and Xi-Hui Liu
- Subjects
0106 biological sciences ,Irrigation ,business.product_category ,biology ,business.industry ,Vinasse ,04 agricultural and veterinary sciences ,biology.organism_classification ,01 natural sciences ,Biotechnology ,Plough ,Agricultural science ,Agriculture ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Cane ,business ,Sugar ,China ,Agronomy and Crop Science ,Mechanization ,010606 plant biology & botany - Abstract
Since 1980s, the Chinese sugar industry has been developing at a fast pace. In the milling year 2007/2008, the total sugar production in China reached 14.83 MT, which was 24.04 % higher than that in previous milling year, and cane sugar production reached 13.67 MT, which was 92.18 % of the total. Guangxi is the largest sugarcane and sugar producer in China, which produced 9.41 MT sugar in 2007/2008 and 8.56 MT sugar in 2013/2014. China has developed a series of unique farming technologies in commercial sugarcane production and has developed an advanced technology system since 1990s. These technologies include deep ploughing and fine preparation of soil, economical fertilization system; trash addition to field, water-saving irrigation, use of pathogen-free healthy seedcane, rational application of vinasse in sugarcane field, chemical control, mechanization for sugarcane management, and comprehensive control of diseases, pests and weeds. The exploitation and comprehensive application of the new sugarcane farming technologies have promoted the Chinese sugar industry to a new level in about every 5 years, and made China become the third biggest sugar-producing country in the world. However, the sugar industry has been experiencing a very difficult time in the last two milling years because of the worldwide low sugar price and the high production cost at the domestic level, which led to a substantial reduction in sugarcane-growing areas and sugar production. Mechanization and the related sugarcane variety selection and farming technology development have become the bottleneck for sustaining the development of the sugar industry in China.
- Published
- 2016
22. Intercropping in Sugarcane Cultivation Influenced the Soil Properties and Enhanced the Diversity of Vital Diazotrophic Bacteria
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Li-Tao Yang, Chang-Ning Li, Zhen Wang, Manoj Kumar Solanki, Fei-Yong Wang, Pratiksha Singh, Tao-Ju Lan, Rajesh Kumar Singh, and Yang-Rui Li
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0106 biological sciences ,Rhizosphere ,education.field_of_study ,biology ,Population ,Intercropping ,04 agricultural and veterinary sciences ,Variovorax ,Pseudomonas nitroreducens ,biology.organism_classification ,01 natural sciences ,Actinobacteria ,Agronomy ,Botany ,040103 agronomy & agriculture ,Variovorax paradoxus ,0401 agriculture, forestry, and fisheries ,Diazotroph ,education ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Soil survey was conducted at four different locations of Guangxi, China to investigate the effect of peanut and soybean inter-cropping in sugarcane cultivation on the soil properties and diazotrophic bacterial diversity. Principal component analysis result shown (PCA) showed difference impact of intercropping and monoculture on the basis of biological and chemical properties of soil. Two-way analysis of variance of soil properties showed a significant interactive effect with locations and cultivation systems. Microbial enumeration results demonstrated that intercropping system enhanced the diazotrophic population. After isolation of diazotrophs, 21 selected bacteria were characterized by siderophore, phosphate, indole acetic acid, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and nitrogenase assay. Among the identified bacteria, isolate MYSP104 showed maximum nitrogenase activity and isolate MYSS78 showed maximum ACC deaminase activity. Partial 16S rRNA gene sequencing results classified these diazotrophs in six phyla (Firmicutes, α, β, Υ-proteobacteria, actinobacteria and bacteroidetes). Diazotrophs such as Bacillus tequilensis, Variovorax paradoxus, Acidovorax facilis, Leucobacter aridicollis, Streptomyces fimicarius and Pseudomonas nitroreducens were reported for the first time from the sugarcane rhizosphere of Guangxi, China. Venn diagram explained that seven bacterial species (Brevibacterium, Burkholderia, Delftia, Leucobacter, Pseudomonas, Sinorhizobium and Variovorax) were recognized with soybean and sugarcane intercropping cultivation systems. This study concluded that intercropping system could enhance the population of N-fixers in soil. Soybean intercropping influenced the soil chemical and biological properties better than peanut. Moreover, the isolated diazotrophs need further characterization and they might be utilized as bio-inoculums for commercial sugarcane production in the future.
- Published
- 2016
23. Comparative analysis of sugarcane root transcriptome in response to the plant growth-promoting Burkholderia anthina MYSP113
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Chang-Ning Li, Mukesh Kumar Malviya, Yang-Rui Li, Rajesh Singh, Manoj Kumar Solanki, Reemon Htun, Pratiksha Singh, Li-Tao Yang, and Krishan K. Verma
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0106 biological sciences ,0301 basic medicine ,Molecular biology ,Gene Expression ,Pathology and Laboratory Medicine ,Biochemistry ,Plant Roots ,01 natural sciences ,Antioxidants ,Transcriptome ,Sequencing techniques ,Gene Expression Regulation, Plant ,Gene expression ,Medicine and Health Sciences ,Cluster Analysis ,Regulation of gene expression ,Multidisciplinary ,Gene Ontologies ,Eukaryota ,Agriculture ,RNA sequencing ,Genomics ,Plants ,Bacterial Pathogens ,Saccharum ,Medical Microbiology ,RNA, Plant ,Medicine ,Metabolic Pathways ,Burkholderia anthina ,Pathogens ,Transcriptome Analysis ,Metabolic Networks and Pathways ,Research Article ,Burkholderia ,Sequence analysis ,Science ,Crops ,Computational biology ,Biology ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Rhizobacteria ,Microbiology ,03 medical and health sciences ,Genetics ,Gene Regulation ,Grasses ,KEGG ,Microbial Pathogens ,Gene ,Bacteria ,Sequence Analysis, RNA ,Organisms ,Biology and Life Sciences ,Computational Biology ,Sugarcane ,Genome Analysis ,biology.organism_classification ,Research and analysis methods ,Metabolism ,Molecular biology techniques ,030104 developmental biology ,Crop Science ,010606 plant biology & botany - Abstract
The diazotrophic Burkholderia anthina MYSP113 is a vital plant growth-promoting bacteria and sugarcane root association. The present study based on a detailed analysis of sugarcane root transcriptome by using the HiSeq-Illumina platform in response to the strain MYSP113. The bacterium was initially isolated from the rhizosphere of sugarcane. To better understand biological, cellular, and molecular mechanisms, a de novo transcriptomic assembly of sugarcane root was performed. HiSeq-Illumina platformwas employed for the sequencing of an overall of 16 libraries at a 2×100 bp configuration. Differentially expressed genes (DEGs) analysis identified altered gene expression in 370 genes (total of 199 up-regulated genes and 171 down-regulated genes). Deciphering the huge datasets, concerning the functioning and production of biological systems, a high throughput genome sequencing analysis was attempted with Gene ontology functional analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The report revealed a total of 148930 unigenes. 70414 (47.28%) of them were annotated successfully to Gene Ontology (GO) terms. 774 at 45 days, 4985 of 30 days and 15 days of 6846 terms were significantly regulated. GO analysis revealed that many genes involved in the metabolic, oxidation-reduction process and biological regulatory processes in response to strain MYSP113 and significantly enriched as compare to the control. Moreover, KEGG enriched results show that differentially expressed genes were classified into different pathway categories involved in various processes, such as nitrogen metabolism, plant hormone signal transduction, etc. The sample correlation analyses could help examine the similarity at the gene expression level. The reliability of the observed differential gene expression patterns was validated with quantitative real-time PCR (qRT-PCR). Additionally, plant enzymes activities such as peroxidase and superoxide dismutase were significantly increased in plant roots after the inoculation of strain MYSP113. The results of the research may help in understanding the plant growth-promoting rhizobacteria and plant interaction.
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- 2020
24. Linkages of Soil Nutrients and Diazotrophic Microbiome under Sugarcane-Legume Intercropping
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Li-Tao Yang, Rajesh Kumar Singh, Yang-Rui Li, Pratiksha Singh, Fei-Yong Wang, Manoj Kumar Solanki, Tao-Ju Lan, Chang-Ning Li, and Zhen Wang
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agricultural_sciences_agronomy ,Agronomy ,Microbial population biology ,Soil nutrients ,food and beverages ,Intercropping ,Microbiome ,Diazotroph ,Biology ,biology.organism_classification ,Legume - Abstract
Intercropping significantly improves land use efficiency and soil fertility. This study examines the impact of three cultivation systems (monoculture sugarcane, peanut-sugarcane and soybean-sugarcane intercropping) on soil properties and diazotrophs. Sugarcane rhizosphere soil was sampled from the farmers’ field. Soil properties and nifH gene abundance were analyzed by high throughput sequencing. Moreover, a total of 436,458 nifH gene sequences were obtained and classified into the 3201 unique operational taxonomic units (OTUs). Maximum unique OTUs resulted with soybean-sugarcane intercropping (
- Published
- 2018
25. Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)
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Li-Tao Yang, Nong Qian, Yang-Rui Li, Chang-Ning Li, and Manoj-Kumar Srivastava
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biology ,food and beverages ,Sequence alignment ,Molecular cloning ,biology.organism_classification ,Biochemistry ,chemistry.chemical_compound ,Open reading frame ,chemistry ,Saccharum officinarum ,Complementary DNA ,Gene expression ,Genetics ,Molecular Biology ,Abscisic acid ,Gene - Abstract
Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment, a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608 amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Joining method indicated that SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including α-helices, β-strands, β-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCED mRNA level and its correlation with O2 – production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O2 – production rate and ABA content after exogenous application of ABA (100 μMol l−1) proved its involvement in pathways providing tolerance to drought stress.
- Published
- 2013
26. Sex-related differences in retinal function in Wistar rats: implications for toxicity and safety studies
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Cheryl Tyszkiewicz, Seo-Kyoung Hwang, Balasubramanian Manickam, Ben Jakubczak, Karen M. Walters, Michael W. Bolt, Rosemary Santos, and Chang-Ning Liu
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retinal function ,toxicity ,Wistar Han ,sex ,electroretinography (ERG) ,Toxicology. Poisons ,RA1190-1270 - Abstract
Introduction: Wistar Han rats are a preferred strain of rodents for general toxicology and safety pharmacology studies in drug development. In some of these studies, visual functional tests that assess for retinal toxicity are included as an additional endpoint. Although the influence of gender on human retinal function has been documented for more than 6 decades, preclinically it is still uncertain if there are differences in retinal function between naïve male and female Wistar Han rats.Methods: In this study, sex-related differences in the retinal function were quantified by analyzing electroretinography (ERG) in 7-9-week-old (n = 52 males and 51 females) and 21–23-week-old Wistar Han rats (n = 48 males and 51 females). Optokinetic tracking response, brainstem auditory evoked potential, ultrasonic vocalization and histology were tested and evaluated in a subset of animals to investigate the potential compensation mechanisms of spontaneous blindness.Results/Discussion: Absence of scotopic and photopic ERG responses was found in 13% of 7-9-week-old (7/52) and 19% of 21–23-week-old males (9/48), but none of female rats (0/51). The averaged amplitudes of rod- and cone-mediated ERG b-wave responses obtained from males were significantly smaller than the amplitudes of the same responses from age-matched females (−43% and −26%, respectively) at 7–9 weeks of age. There was no difference in the retinal and brain morphology, brainstem auditory responses, or ultrasonic vocalizations between the animals with normal and abnormal ERGs at 21–23 weeks of age. In summary, male Wistar Han rats had altered retinal responses, including a complete lack of responses to test flash stimuli (i.e., blindness), when compared with female rats at 7–9 and 21–23 weeks of age. Therefore, sex differences should be considered when using Wistar Han rats in toxicity and safety pharmacology studies with regards to data interpretation of retinal functional assessments.
- Published
- 2023
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27. Differential expression profiles and pathways of genes in sugarcane leaf at elongation stage in response to drought stress
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Nong Qian, Qiang Liang, Wang Weizan, Liu Xiaoyan, Xie Jinlan, Manoj Kumar Solanki, Li-Tao Yang, Yang-Rui Li, Li Yijie, and Chang-Ning Li
- Subjects
0106 biological sciences ,0301 basic medicine ,Drought tolerance ,Biology ,01 natural sciences ,Homology (biology) ,Article ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Gene expression ,Botany ,Gene ,Plant Proteins ,Genetics ,Regulation of gene expression ,Multidisciplinary ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Gene Expression Regulation, Developmental ,Water ,Droughts ,Saccharum ,Gene expression profiling ,Plant Leaves ,030104 developmental biology ,Gene Ontology ,Gene chip analysis ,Signal transduction ,010606 plant biology & botany ,Signal Transduction - Abstract
Water stress causes considerable yield losses in sugarcane. To investigate differentially expressed genes under water stress, a pot experiment was performed with the sugarcane variety GT21 at three water-deficit levels (mild, moderate and severe) during the elongation stage and gene expression was analyzed using microarray technology. Physiological parameters of sugarcane showed significant alterations in response to drought stress. Based on the expression profile of 15,593 sugarcane genes, 1,501 (9.6%) genes were differentially expressed under different water-level treatments; 821 genes were upregulated and 680 genes were downregulated. A gene similarity analysis showed that approximately 62.6% of the differentially expressed genes shared homology with functional proteins. In a Gene Ontology (GO) analysis, 901 differentially expressed genes were assigned to 36 GO categories. Moreover, 325 differentially expressed genes were classified into 101 pathway categories involved in various processes, such as the biosynthesis of secondary metabolites, ribosomes, carbon metabolism, etc. In addition, some unannotated genes were detected; these may provide a basis for studies of water-deficit tolerance. The reliability of the observed expression patterns was confirmed by RT-PCR. The results of this study may help identify useful genes for improving drought tolerance in sugarcane.
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- 2016
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28. Bearing Fault Diagnosis Using Gaussian Mixture Models (GMMs)
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Jian Wei Sun, Gang Yu, and Chang Ning Li
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Engineering ,Bearing (mechanical) ,business.industry ,Feature vector ,Wavelet transform ,Pattern recognition ,General Medicine ,Mixture model ,law.invention ,Vibration ,Experimental system ,law ,Computer Science::Computer Vision and Pattern Recognition ,Ball (bearing) ,Artificial intelligence ,business ,Computer Science::Operating Systems ,Classifier (UML) - Abstract
This paper presents a novel method for bearing fault diagnosis based on wavelet transform and Gaussian mixture models (GMMs). Vibration signals for normal bearings, bearings with inner race faults, outer race faults and ball faults were acquired from a motor-driven experimental system. The wavelet transform was used to process the vibration signals and to generate feature vectors. GMMs were trained and used as a diagnostic classifier. Experimental results have shown that GMMs can reliably classify different fault conditions and have a better classification performance as compared to the multilayer perceptron neural networks.
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- 2007
29. A Cluster-Based Wavelet Feature Extraction Method for Machine Fault Diagnosis
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Gang Yu, Chang Ning Li, and Sagar Kamarthi
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Discrete wavelet transform ,Engineering ,Artificial neural network ,business.industry ,Feature vector ,Stationary wavelet transform ,Feature extraction ,Pattern recognition ,General Medicine ,Wavelet ,Discriminative model ,Entropy (information theory) ,Artificial intelligence ,business - Abstract
In this paper, a cluster-based feature extraction from the coefficients of discrete wavelet transform is proposed for machine fault diagnosis. The proposed approach first divides the matrix of wavelet coefficients into clusters that are centered around the discriminative coefficient positions identified by an unsupervised procedure based on the entropy value of coefficients from a set of representative signals. The features that contain the informative attributes of the signals are computed from the energy content of so obtained clusters. Then machine faults are diagnosed based on these feature vectors using a neural network. The experimental results from the application on bearing fault diagnosis have shown that the proposed approach is able to effectively extract important intrinsic information content of the test signals, and increase the overall fault diagnostic accuracy as compared to conventional methods.
- Published
- 2007
30. Increases in GFAP immunoreactive astrocytes in the cerebellar molecular layer of young adult CBA/J mice
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Cheryl Tyszkiewicz, Ingrid D Pardo, Hayley N Ritenour, Chang-Ning Liu, and Chris Somps
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CBA/J mice ,cerebellum ,Astrocyte ,Bergmann glia ,Glial fibrillary acidic protein ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background CBA/J mice are standard experimental animals in auditory studies, and age-related changes in auditory pathways are well documented. However, changes in locomotion-related brain regions have not been systematically explored. Results We showed an increase in immunoreactivity for glial fibrillary acidic protein (GFAP) in the cerebellar molecular layer associated with Purkinje cells in mice at 24 weeks of age but not in the younger mice. Increased GFAP immunoreactivity appeared in the form of clusters and distributed multifocally consistent with hyperplasia of astrocytes that were occasionally associated with Purkinje cell degeneration. Three out of 12 animals at 16 and 24 weeks of age exhibited pre-convulsive clinical signs. Two of these 3 animals also showed increased GFAP immunoreactivity in the cerebellum. Rotarod behavioral assessments indicated decreased performance at 24 weeks of age. Conclusions These results suggest minimal to mild reactive astrocytosis likely associated with Purkinje cell degeneration in the cerebellum at 24 weeks of age in CBA/J mice. These findings should be taken into consideration prior to using this mouse strain for studying neuroinflammation or aging.
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- 2021
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31. Screening of Differentially Expressed Genes and Analysis of Plant Hormones Related Genes under Water Stress in Sugarcane
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Chang-Ning LI, Jin-Lan XIE, Wei-Zan WANG, Qiang LIANG, Yi-Jie LI, Wen-Bin DONG, Xiao-Yan LIU, Li-Tao YANG, and Yang-Rui LI
- Subjects
Plant Science ,Agronomy and Crop Science ,Biotechnology - Published
- 2015
32. Introduction of gloved hand to cage induces 22-kHz ultrasonic vocalizations in male albino rats.
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Seo-Kyoung Hwang, Cheryl Tyszkiewicz, Melissa Dragon, Kimberly Navetta, Rebecca Ferreira, and Chang-Ning Liu
- Subjects
Medicine ,Science - Abstract
Rodents emit ultrasonic vocalizations (USVs) above the human hearing threshold of ~ 20 kHz to communicate emotional states and to coordinate their social interactive behavior. Twenty-two kHz USVs emitted by adult rats have been reported in a variety of aversive social and behavioral situations. They occur not only under painful or restraining conditions but can also be evoked by gentle cutaneous touch or airflow. This study aimed to test if placement of a human hand in a cage can evoke 22-kHz USVs. It was found that 36% of the adult male Sprague-Dawley and 13% of the adult male Wistar Han rats emitted 22-kHz USVs when a gloved hand was introduced into the cages. Average vocalization onset latencies were 5.0 ± 4.4 s (Sprague-Dawley) and 7.4 ± 4.0 s (Wistar Han) and the USVs had a stable frequency (22 kHz) across the calls, ranging from 0.1 to 2.3 seconds in duration. Surprisingly, no 22-kHz USVs were found in any female Wistar Han rats tested. To further explore the mechanisms underlying this observation, we compared retinal function, basal serum corticosterone, and testosterone levels between the 22-kHz USV responders and non-responders. None of these parameters or endpoints showed any significant differences between the two cohorts. The results suggest that the introduction of a gloved-hand inside the cage can trigger adult male albino rats to emit 22-kHz ultrasonic vocalizations. This response should be considered in USV studies and animal welfare.
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- 2022
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33. Comparison of the Efficacy of Nonsteroidal Anti-Inflammatory Drugs and Opioids in the Treatment of Acute Renal Colic: A Systematic Review and Meta-Analysis
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Xie-Yuan Leng, Chang-Ning Liu, Shi-Chan Wang, Hao-Dong Peng, De-Guang Wang, and Hai-Feng Pan
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renal colic ,NSAIDs ,nonsteroidal drugs ,opioids ,meta-analysis ,systematic review ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Although multiple randomized controlled trials (RCTs) and systematic review and meta-analysis were performed to investigate the efficiency and safety of nonsteroidal anti-inflammatory drugs (NSAIDs) and opioids in the treatment of acute renal colic, the therapeutic regimen of renal colic is still controversial. Therefore, the aim of this study was to derive a more concise comparison of the effectiveness and safety between NSAIDs and opioids in the treatment for patients with acute renal colic by a systematic review and meta-analysis.Design: We searched PubMed, Embase, and Cochrane Central Register of controlled trials for seeking eligible studies. The pooled mean difference (MD) or risk ratio (RR) with 95% confidence interval (CI) was calculated using the random effects model. The primary outcome was assessed according to the Grading of Recommendations Assessment, Development and Evaluation.Results: A total of 18 studies involving 3,121 participants were included in the systematic review and meta-analysis. No significant difference between the NSAID and opioid groups was observed, with changes in the visual analog scale (VAS) at 0–30 min (MD = 0.79, 95% CI: −0.51, 2.10). NSAIDs in the form of intravenous administration (IV) had no better effect on the changes in the VAS at 0–30 min, when compared to opioids (MD = 1.25, 95% Cl: −4.81, 7.3). The NSAIDs group in the form of IV had no better outcome compared to the opioids group, as well as the VAS at 30 min (MD = −1.18, 95% Cl: −3.82, 1.45; MD = −2.3, 95% Cl: −5.02, 0.42, respectively). Moreover, similar results of this outcome were also seen with the VAS at 45 min (MD = −1.36, 95% Cl: −5.24, 2.52). Besides, there was a statistical difference in the incidence of later rescue (RR = 0.76, 95% CI: 0.66, 0.89), drug-related adverse events (RR = 0.44, 95% CI: 0.27, 0.71), and vomiting (RR = 0.68, 95% CI: 0.49, 0.96).Conclusion: There is no significant difference between the NSAIDs and opioids in the treatment of renal colic in many outcomes (e.g., the VAS over different periods using different injection methods at 30 and 60 min), which has been focused on in this study. However, the patients who were treated using NSAIDs by clinicians can benefit from fewer side effects.
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- 2022
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34. Cloning and Expression Analysis of Abscisic Acid Signal Transduction KeyEnzyme Gene SoSnRK2.1 from Sugarcane
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Qin-Liang Tan, Chang-Ning Li, Li-Tao Yang, and Yang-Rui Li
- Subjects
Cloning ,Biochemistry ,Expression analysis ,Plant Science ,Biology ,Abscisic acid signal transduction ,Agronomy and Crop Science ,Molecular biology ,Biotechnology - Published
- 2013
35. Automated monitoring of respiratory rate as a novel humane endpoint: A refinement in mouse metastatic lung cancer models.
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Caroline B Winn, Seo-Kyoung Hwang, Jeffrey Morin, Crystal T Bluette, Balasubramanian Manickam, Ziyue K Jiang, Anand Giddabasappa, Chang-Ning Liu, and Kristin Matthews
- Subjects
Medicine ,Science - Abstract
In oncology research, while xenograft tumor models are easily visualized and humane endpoints can be clearly defined, metastatic tumor models are often based on more subjective clinical observations as endpoints. This study aimed at identifying objective non-invasive criteria for predicting imminent distress and mortality in metastatic lung tumor-bearing mice. BALB/c and C57BL/6 mice were inoculated with CT26 or B16F10 cells, respectively. The mice were housed in Vium smart cages to continuously monitor and stream respiratory rate and locomotion for up to 28 days until scheduled euthanasia or humane endpoint criteria were met. Body weight and body temperature were measured during the study. On days 11, 14, 17 and 28, lungs of subsets of animals were microCT imaged in vivo to assess lung metastasis progression and then euthanized for lung microscopic evaluations. Beginning at day 21, most tumor-bearing animals developed increased respiratory rates followed by decreased locomotion 1-2 days later, compared with the baseline values. Increases in respiratory rate did not correlate to surface tumor nodule counts or lung weight. Body weight measurement did not show significant changes from days 14-28 in either tumor-bearing or control animals. We propose that increases in respiratory rate (1.3-1.5 X) can be used to provide an objective benchmark to signal the need for increased clinical observations or euthanasia. Adoption of this novel humane endpoint criterion would allow investigators time to collect tissue samples prior to spontaneous morbidity or death and significantly reduce the distress of mice in the terminal stages of these metastatic lung tumor models.
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- 2021
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36. Circulating microRNA and automated motion analysis as novel methods of assessing chemotherapy-induced peripheral neuropathy in mice.
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Qinghai Peng, Jordan Mechanic, Ahmed Shoieb, Ingrid D Pardo, Laura Schaevitz, Judith Fenyk-Melody, Allison Vitsky, Magalie Boucher, Chris Somps, Jon C Cook, and Chang-Ning Liu
- Subjects
Medicine ,Science - Abstract
Chemotherapy-induced peripheral neuropathy (CiPN) is a serious adverse effect in the clinic, but nonclinical assessment methods in animal studies are limited to labor intensive behavioral tests or semi-quantitative microscopic evaluation. Hence, microRNA (miRNA) biomarkers and automated in-life behavioral tracking were assessed for their utility as non-invasive methods. To address the lack of diagnostic biomarkers, we explored miR-124, miR-183 and miR-338 in a CiPN model induced by paclitaxel, a well-known neurotoxic agent. In addition, conventional and Vium's innovative Digital Vivarium technology-based in-life behavioral tests and postmortem microscopic examination of the dorsal root ganglion (DRG) and the sciatic nerve were performed. Terminal blood was collected on days 8 or 16, after 20 mg/kg paclitaxel was administered every other day for total of 4 or 7 doses, respectively, for plasma miRNA quantification by RT-qPCR. DRG and sciatic nerve samples were collected from mice sacrificed on day 16 for miRNA quantification. Among the three miRNAs analyzed, only miR-124 was statistically significantly increased (5 fold and 10 fold on day 8 and day 16, respectively). The increase in circulating miR-124 correlated with cold allodynia and axonal degeneration in both DRG and sciatic nerve. Automated home cage motion analysis revealed for the first time that nighttime motion was significantly decreased (P < 0.05) in paclitaxel-dosed animals. Although both increase in circulating miR-124 and decrease in nighttime motion are compelling, our results provide positive evidence warranting further testing using additional peripheral nerve toxicants and diverse experimental CiPN models.
- Published
- 2019
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