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Your search keyword '"Chang, Tse-Shao"' showing total 19 results
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19 results on '"Chang, Tse-Shao"'

7. Detection of Hepatocellular Carcinoma in an Orthotopic Patient-Derived Xenograft with an Epithelial Cell Adhesion Molecule-Specific Peptide.

Author

Wu, Xiaoli, Feng, Shuo, Chang, Tse-Shao, Zhang, Ruoliu, Jaiswal, Sangeeta, Choi, Eun-Young K., Duan, Yuting, Jiang, Hui, and Wang, Thomas D.

Subjects
EPITHELIAL cells, IN vitro studies, CARRIER proteins, RESEARCH funding, CELL adhesion molecules, EARLY detection of cancer, XENOGRAFTS, TUMOR markers, CANCER patients, NEAR infrared spectroscopy, FLUORESCENT antibody technique, PEPTIDES, GENE expression, CELL lines, MICE, ANIMAL experimentation, STAINS & staining (Microscopy), LIVER, HEPATOCELLULAR carcinoma
Abstract

Simple Summary: Hepatocellular carcinoma (HCC) is the most common form of liver cancer, and is occurring with greater frequency worldwide. Better methods are needed to detect this disease at an early time point and help guide surgery. A peptide, or protein fragment, has been developed to bind specifically to EpCAM, a molecular target expressed uniquely by cancer cells and to concentrate in tumors. This peptide was labeled with a near-infrared fluorophore, and showed strong binding to HCC cells and in a live animal model using viable human HCC tumors. The peptide was found to be stable in serum, and showed high uptake in HCC tumors with no signs of toxicity. The peptide also bound to cancer that spread throughout the liver and into the lungs, and was more concentrated in tumors than in other organs. These results support the potential usefulness of the EpCAM peptide to detect and remove HCC. Hepatocellular carcinoma (HCC) has emerged as a major contributor to the worldwide cancer burden. Improved methods are needed for early cancer detection and image-guided surgery. Peptides have small dimensions that can overcome delivery challenges to achieve high tumor concentrations and deep penetration. We used phage display methods to biopan against the extra-cellular domain of the purified EpCAM protein, and used IRDye800 as a near-infrared (NIR) fluorophore. The 12-mer sequence HPDMFTRTHSHN was identified, and specific binding to EpCAM was validated with HCC cells in vitro. A binding affinity of kd = 67 nM and onset of k = 0.136 min−1 (7.35 min) were determined. Serum stability was measured with a half-life of T1/2 = 2.6 h. NIR fluorescence images showed peak uptake in vivo by human HCC patient-derived xenograft (PDX) tumors at 1.5 h post-injection. Also, the peptide was able to bind to foci of local and distant metastases in liver and lung. Peptide biodistribution showed high uptake in tumor versus other organs. No signs of acute toxicity were detected during animal necropsy. Immunofluorescence staining of human liver showed specific binding to HCC compared with cirrhosis, adenoma, and normal specimens. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Detection of Barrett’s neoplasia with a near-infrared fluorescent heterodimeric peptide

Author

Chen, Jing, additional, Jiang, Yang, additional, Chang, Tse-Shao, additional, Rubenstein, Joel H., additional, Kwon, Richard S., additional, Wamsteker, Erik J., additional, Prabhu, Anoop, additional, Zhao, Lili, additional, Appelman, Henry D., additional, Owens, Scott R., additional, Beer, David G., additional, Turgeon, D. Kim, additional, Seibel, Eric J., additional, and Wang, Thomas D., additional

Published
2022
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14. Detection of Barrett's Neoplasia with Near-Infrared Fluorescent Heterodimeric Peptide: Feasibility Results from a Phase 1 Study

Author

Chen, Jing, primary, Chang, Tse-Shao, additional, Rubenstein, Joel H., additional, Kwon, Richard S., additional, Wamsteker, Erik J., additional, Prabhu, Anoop, additional, Appelman, Henry D., additional, Owens, Scott R., additional, Beer, David G., additional, Turgeon, D. Kim, additional, Seibel, Eric J., additional, and Wang, Thomas D., additional

Published
2021
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16. Multiplexed endoscopic imaging of Barrett’s neoplasia using targeted fluorescent heptapeptides in a phase 1 proof-of-concept study

Author

Chen, Jing, primary, Jiang, Yang, additional, Chang, Tse-Shao, additional, Joshi, Bishnu, additional, Zhou, Juan, additional, Rubenstein, Joel H, additional, Wamsteker, Erik J, additional, Kwon, Richard S, additional, Appelman, Henry, additional, Beer, David G, additional, Turgeon, Danielle K, additional, Seibel, Eric J, additional, and Wang, Thomas D, additional

Published
2020
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