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4. A cellular resolution atlas of Broca’s area

Author

Costantini, Irene, primary, Morgan, Leah, additional, Yang, Jiarui, additional, Balbastre, Yael, additional, Varadarajan, Divya, additional, Pesce, Luca, additional, Scardigli, Marina, additional, Mazzamuto, Giacomo, additional, Gavryusev, Vladislav, additional, Castelli, Filippo Maria, additional, Roffilli, Matteo, additional, Silvestri, Ludovico, additional, Laffey, Jessie, additional, Raia, Sophia, additional, Varghese, Merina, additional, Wicinski, Bridget, additional, Chang, Shuaibin, additional, Chen, Ichun Anderson, additional, Wang, Hui, additional, Cordero, Devani, additional, Vera, Matthew, additional, Nolan, Jackson, additional, Nestor, Kimberly, additional, Mora, Jocelyn, additional, Iglesias, Juan Eugenio, additional, Garcia Pallares, Erendira, additional, Evancic, Kathryn, additional, Augustinack, Jean C., additional, Fogarty, Morgan, additional, Dalca, Adrian V., additional, Frosch, Matthew P., additional, Magnain, Caroline, additional, Frost, Robert, additional, van der Kouwe, Andre, additional, Chen, Shih-Chi, additional, Boas, David A., additional, Pavone, Francesco Saverio, additional, Fischl, Bruce, additional, and Hof, Patrick R., additional

Published
2023
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5. Multi‐Scale Label‐Free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two‐Photon Microscopy.

Author

Chang, Shuaibin, Yang, Jiarui, Novoseltseva, Anna, Abdelhakeem, Ayman, Hyman, Mackenzie, Fu, Xinlei, Li, Chenglin, Chen, Shih‐Chi, Augustinack, Jean C., Magnain, Caroline, Fischl, Bruce, Mckee, Ann C., Boas, David A., Chen, Ichun Anderson, and Wang, Hui

Subjects
*OPTICAL coherence tomography, *OPTICAL polarization, *BRAIN imaging, *MICROSCOPY, *IMAGING systems, *DIFFUSION tensor imaging
Abstract

The study of aging and neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation‐free reconstruction. Here, the authors describe an integrated serial sectioning polarization‐sensitive optical coherence tomography (PSOCT) and two photon microscopy (2PM) system to provide label‐free multi‐contrast imaging of intact brain structures, including scattering, birefringence, and autofluorescence of human brain tissue. The authors demonstrate high‐throughput reconstruction of 4 × 4 × 2cm3 sample blocks and simple registration between PSOCT and 2PM images that enable comprehensive analysis of myelin content, vascular structure, and cellular information. The high‐resolution 2PM images provide microscopic validation and enrichment of the cellular information provided by the PSOCT optical properties on the same sample, revealing the densely packed fibers, capillaries, and lipofuscin‐filled cell bodies in the cortex and white matter. It is shown that the imaging system enables quantitative characterization of various pathological features in aging process, including myelin degradation, lipofuscin accumulation, and microvascular changes, which opens up numerous opportunities in the study of neurodegenerative diseases in the future. [ABSTRACT FROM AUTHOR]

Published
2023
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6. Multi-Scale Label-free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy

Author

Chang, Shuaibin, primary, Yang, Jiarui, additional, Novoseltseva, Anna, additional, Fu, Xinlei, additional, Li, Chenglin, additional, Chen, Shih-Chi, additional, Augustinack, Jean C., additional, Magnain, Caroline, additional, Fischl, Bruce, additional, McKee, Ann C., additional, Boas, David A., additional, Chen, Ichun Anderson, additional, and Wang, Hui, additional

Published
2023
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7. Volumetric Characterization of Microvasculature in Ex Vivo Human Brain Samples By Serial Sectioning Optical Coherence Tomography

Author

Yang, Jiarui, primary, Chang, Shuaibin, additional, Chen, Ichun Anderson, additional, Kura, Sreekanth, additional, Rosen, Grace A., additional, Saltiel, Nicole A., additional, Huber, Bertrand R., additional, Varadarajan, Divya, additional, Balbastre, Yael, additional, Magnain, Caroline, additional, Chen, Shih-chi, additional, Fischl, Bruce, additional, McKee, Ann C., additional, Boas, David A., additional, and Wang, Hui, additional

Published
2022
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10. Imaging post-mortem neurodegenerative human brains with serial sectioning optical coherence tomography

Author

Yang, Jiarui, primary, Chang, Shuaibin, additional, Rosen, Grace A., additional, Saltiel, Nicole A., additional, Wang, Hui, additional, Magnain, Caroline, additional, Chen, Shih-chi, additional, Fischl, Bruce, additional, Huber, Bertrand R., additional, McKee, Ann C., additional, Chen, Ichun Anderson, additional, and Boas, David A., additional

Published
2022
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11. Refractive-index matching enhanced polarization sensitive optical coherence tomography quantification in human brain tissue

Author

Liu, Chao J., primary, Ammon, William, additional, Jones, Robert J., additional, Nolan, Jackson, additional, Wang, Ruopeng, additional, Chang, Shuaibin, additional, Frosch, Matthew P., additional, Yendiki, Anastasia, additional, Boas, David A., additional, Magnain, Caroline, additional, Fischl, Bruce, additional, and Wang, Hui, additional

Published
2021
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12. A multimodal imaging and analysis pipeline for creating a cellular census of the human cerebral cortex

Author

Costantini, Irene, primary, Morgan, Leah, additional, Yang, Jiarui, additional, Balbastre, Yael, additional, Varadarajan, Divya, additional, Pesce, Luca, additional, Scardigli, Marina, additional, Mazzamuto, Giacomo, additional, Gavryusev, Vladislav, additional, Castelli, Filippo Maria, additional, Roffilli, Matteo, additional, Silvestri, Ludovico, additional, Laffey, Jessie, additional, Raia, Sophia, additional, Varghese, Merina, additional, Wicinski, Bridget, additional, Chang, Shuaibin, additional, Chen I-Chun, Anderson, additional, Wang, Hui, additional, Cordero, Devani, additional, Vera, Matthew, additional, Nolan, Jackson, additional, Nestor, Kim, additional, Mora, Jocelyn, additional, Iglesias, Juan Eugenio, additional, Pallares, Erendira Garcia, additional, Evancic, Kathryn, additional, Augustinack, Jean, additional, Fogarty, Morgan, additional, Dalca, Adrian V., additional, Frosch, Matthew, additional, Magnain, Caroline, additional, Frost, Robert, additional, van der Kouwe, Andre, additional, Chen, Shih-Chi, additional, Boas, David A., additional, Pavone, Francesco Saverio, additional, Fischl, Bruce, additional, and Hof, Patrick R., additional

Published
2021
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16. Feature enhancement volumetric visualization of human brain sample with serial sectioning spectral domain optical coherence tomography

Author

Yang, Jiarui, primary, Chang, Shuaibin, additional, Chen, Ichun Anderson, additional, Varadarajan, Divya, additional, Wang, Hui, additional, Magnain, Caroline, additional, Fischl, Bruce, additional, Costantini, Irene, additional, Pavone, Francesco, additional, McKee, Ann C., additional, Chen, Shih-Chi, additional, and Boas, David A., additional

Published
2021
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17. Improving the characterization of ex vivo human brain optical properties using high numerical aperture optical coherence tomography by spatially constraining the confocal parameters

Author

Yang, Jiarui, primary, Chen, Ichun Anderson, additional, Chang, Shuaibin, additional, Tang, Jianbo, additional, Lee, Blaire, additional, Kılıç, Kıvılcım, additional, Sunil, Smrithi, additional, Wang, Hui, additional, Varadarajan, Divya, additional, Magnain, Caroline, additional, Chen, Shih-Chi, additional, Costantini, Irene, additional, Pavone, Francesco, additional, Fischl, Bruce, additional, and Boas, David A., additional

Published
2020
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18. Parametrizing confocal parameters for characterizing the optical properties of human brain tissue using high numerical aperture optical coherence tomography

Author

Yang, Jiarui, primary, Chen, Ichun Anderson, additional, Chang, Shuaibin, additional, Tang, Jianbo, additional, Lee, Blaire, additional, Kılıç, Kıvılcım, additional, Wang, Hui, additional, Magnain, Caroline, additional, Chen, Shih-chi, additional, Fischl, Bruce, additional, and Boas, David A., additional

Published
2020
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20. Serial sectioning PSOCT and 2PM for imaging post-mortem human brain and neurodegeneration

Author

Chang, Shuaibin

Subjects
Electrical engineering
Abstract

The study of aging and neurodegenerative processes in the human brain necessitates a comprehensive understanding of its myeloarchitectonic, cytoarchitectonic, and vascular structures. While recent computational advances have enabled volumetric reconstruction of the human brain using stained slices, the standard histological processing methods have often led to tissue distortions and loss, making deformation-free reconstruction challenging. Therefore, the development of a multi-scale and volumetric imaging technique that can accurately measure multiple structures within the intact brain would be a significant technical breakthrough. In this work, we present the development of an integrated approach that combines serial sectioning Polarization Sensitive Optical Coherence Tomography (PSOCT) and Two Photon Microscopy (2PM) to provide label-free multi-contrast imaging of human brain tissue. Our method allows for the simultaneous visualization of scattering, birefringence, and autofluorescence properties of the post-mortem human brain. By utilizing high-throughput reconstruction of 4x4x2cm3 sample blocks and simple registration of PSOCT and 2PM images, we enable comprehensive analysis of myelin content, cellular information, and vascular structure. PSOCT provides mesoscopic images and enables quantitative measurement of those brain structures, while 2PM provide complementary microscopic validation and enrichment of cellular and capillary information. This combined approach reveals myelin density and structure maps of the whole brain block and supplies intricate vessel and capillary networks as well as lipofuscin-filled cell soma across cortical regions, providing insights into the myeloarchitectural, cellular and vascular changes associated with neurodegenerative diseases such as Alzheimer's disease (AD) and Chronic Traumatic Encephalopathy (CTE).

Published
2023

23. Enhanced Multiscale Human Brain Imaging by Semi-supervised Digital Staining and Serial Sectioning Optical Coherence Tomography.

Author

Cheng S, Chang S, Li Y, Novoseltseva A, Lin S, Wu Y, Zhu J, McKee AC, Rosene DL, Wang H, Bigio IJ, Boas DA, and Tian L

Abstract

A major challenge in neuroscience is to visualize the structure of the human brain at different scales. Traditional histology reveals micro- and meso-scale brain features, but suffers from staining variability, tissue damage and distortion that impedes accurate 3D reconstructions. Here, we present a new 3D imaging framework that combines serial sectioning optical coherence tomography (S-OCT) with a deep-learning digital staining (DS) model. We develop a novel semi-supervised learning technique to facilitate DS model training on weakly paired images. The DS model performs translation from S-OCT to Gallyas silver staining. We demonstrate DS on various human cerebral cortex samples with consistent staining quality. Additionally, we show that DS enhances contrast across cortical layer boundaries. Furthermore, we showcase geometry-preserving 3D DS on cubic-centimeter tissue blocks and visualization of meso-scale vessel networks in the white matter. We believe that our technique offers the potential for high-throughput, multiscale imaging of brain tissues and may facilitate studies of brain structures., Competing Interests: Competing interests: The authors declare that they have no competing interests.

Published
2024
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24. Multi-Scale Label-free Human Brain Imaging with Integrated Serial Sectioning Polarization Sensitive Optical Coherence Tomography and Two-Photon Microscopy.

Author

Chang S, Yang J, Novoseltseva A, Fu X, Li C, Chen SC, Augustinack JC, Magnain C, Fischl B, Mckee AC, Boas DA, Chen IA, and Wang H

Abstract

The study of neurodegenerative processes in the human brain requires a comprehensive understanding of cytoarchitectonic, myeloarchitectonic, and vascular structures. Recent computational advances have enabled volumetric reconstruction of the human brain using thousands of stained slices, however, tissue distortions and loss resulting from standard histological processing have hindered deformation-free reconstruction of the human brain. The development of a multi-scale and volumetric human brain imaging technique that can measure intact brain structure would be a major technical advance. Here, we describe the development of integrated serial sectioning Polarization Sensitive Optical Coherence Tomography (PSOCT) and Two Photon Microscopy (2PM) to provide label-free multi-contrast imaging, including scattering, birefringence and autofluorescence of human brain tissue. We demonstrate that high-throughput reconstruction of 4×4×2cm 3 sample blocks and simple registration of PSOCT and 2PM images enable comprehensive analysis of myelin content, vascular structure, and cellular information. We show that 2 μm in-plane resolution 2PM images provide microscopic validation and enrichment of the cellular information provided by the PSOCT optical property maps on the same sample, revealing the sophisticated capillary networks and lipofuscin filled cell bodies across the cortical layers. Our method is applicable to the study of a variety of pathological processes, including demyelination, cell loss, and microvascular changes in neurodegenerative diseases such as Alzheimer's disease (AD) and Chronic Traumatic Encephalopathy (CTE).

Published
2023
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