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201 results on '"Chang, Guohui"'

3. Dysbiosis of oropharyngeal microbiome and antibiotic resistance in hospitalized COVID‐19 patients

Author

Wu, Jiani, primary, Liu, Wei, additional, Zhu, Lin, additional, Li, Nina, additional, Luo, Gengyan, additional, Gu, Ming, additional, Peng, Minwu, additional, Zeng, Shike, additional, Wu, Shu, additional, Zhang, Shengze, additional, Chen, Qiqi, additional, Cai, Meiqi, additional, Cao, Wei, additional, Jiang, Ying, additional, Luo, Chuming, additional, Tian, Dechao, additional, Shi, Mang, additional, Shu, Yuelong, additional, Chang, Guohui, additional, and Luo, Huanle, additional

Published
2023
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29. Attenuation of mouse hepatitis virus by deletion of the LLRKxGxKG region of Nsp1.

Author

Lin Lei, Sun Ying, Luo Baojun, Yang Yi, He Xiang, Su Wenli, Sun Zounan, Guo Deyin, Zhu Qingyu, Liu Jingmei, and Chang Guohui

Subjects
Medicine, Science
Abstract

Coronaviruses are a family of large positive-sense RNA viruses that are responsible for a wide range of important veterinary and human diseases. Nsp1 has been shown to have an important role in the pathogenetic mechanisms of coronaviruses in vivo. To assess the function of a relatively conserved domain (LLRKxGxKG) of MHV nsp1, a mutant virus, MHV-nsp1-27D, with a 27 nts (LLRKxGxKG) deletion in nsp1, was constructed using a reverse genetic system with a vaccinia virus vector. The mutant virus had similar growth kinetics to MHV-A59 wild-type virus in 17CI-1 cells, but was highly attenuated in vivo. Moreover, the mutant virus completely protected C57BL/6 mice from a lethal MHV-A59 challenge. To further analyze the mechanism of the attenuation of the mutant virus, changes in reporter gene expression were measured in nsp1- or nsp1-27D-expressing cells; the results showed that nsp1 inhibited reporter gene expression controlled by different promoters, but that this inhibition was reduced for nsp1-27D. The research in vivo and in vitro suggests that the LLRKxGxKG region of nsp1 may play an important role in this process.

Published
2013
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31. A complete sequence and comparative analysis of a SARS-associated virus (Isolate BJ01)

Author

Qin, E’de, Zhu, Qingyu, Yu, Man, Fan, Baochang, Chang, Guohui, Si, Bingyin, Yang, Bao’an, Peng, Wenming, Jiang, Tao, Liu, Bohua, Deng, Yongqiang, Liu, Hong, Zhang, Yu, Wang, Cui’e, Li, Yuquan, Gan, Yonghua, Li, Xiaoyu, Lü, Fushuang, Tan, Gang, Cao, Wuchun, Yang, Ruifu, Wang, Jian, Li, Wei, Xu, Zuyuan, Li, Yan, Wu, Qingfa, Lin, Wei, Chen, Weijun, Tang, Lin, Deng, Yajun, Han, Yujun, Li, Changfeng, Lei, Meng, Li, Guoqing, Li, Wenjie, Lü, Hong, Shi, Jianping, Tong, Zongzhong, Zhang, Feng, Li, Songgang, Liu, Bin, Liu, Siqi, Dong, Wei, Wang, Jun, Wong, Gane K-S, Yu, Jun, and Yang, Huanming

Published
2003
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32. Immunogenicity and protective efficacy in monkeys of purified inactivated Vero-cell SARS vaccine

Author

Qin, Ede, Shi, Huiying, Tang, Lin, Wang, Cuie, Chang, Guohui, Ding, Zhifen, Zhao, Kai, Wang, Jian, Chen, Ze, Yu, Man, Si, Bingyin, Liu, Jianyuan, Wu, Donglai, Cheng, Xiaojie, Yang, Baoan, Peng, Wenming, Meng, Qingwen, Liu, Bohua, Han, Weiguo, Yin, Xunnan, Duan, Hongyuan, Zhan, Dawei, Tian, Long, Li, Shuangli, Wu, Jinsong, Tan, Gang, Li, Yi, Li, Yuchuan, Liu, Yonggang, Liu, Hong, Lv, Fushuang, Zhang, Yu, Kong, Xiangang, Fan, Baochang, Jiang, Tao, Xu, Shuli, Wang, Xiaomei, Li, Changwen, Wu, Xiaohong, Deng, Yongqiang, Zhao, Min, and Zhu, Qingyu

Published
2006
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37. A Genome Sequence of Novel SARS-CoV Isolates: the Genotype, GD-Ins29, Leads to a Hypothesis of Viral Transmission in South China

Author

Qin, E’de, He, Xionglei, Tian, Wei, Liu, Yong, Li, Wei, Wen, Jie, Wang, Jingqiang, Fan, Baochang, Wu, Qingfa, Chang, Guohui, Cao, Wuchun, Xu, Zuyuan, Yang, Ruifu, Wang, Jing, Yu, Man, Li, Yan, Xu, Jing, Si, Bingyin, Hu, Yongwu, Peng, Wenming, Tang, Lin, Jiang, Tao, Shi, Jianping, Ji, Jia, Zhang, Yu, Ye, Jia, Wang, Cui’e, Han, Yujun, Zhou, Jun, Deng, Yajun, Li, Xiaoyu, Hu, Jianfei, Wang, Caiping, Yan, Chunxia, Zhang, Qingrun, Bao, Jingyue, Li, Guoqing, Chen, Weijun, Fang, Lin, Li, Changfeng, Lei, Meng, Li, Dawei, Tong, Wei, Tian, Xiangjun, Wang, Jin, Zhang, Bo, Zhang, Haiqing, Zhang, Yilin, Zhao, Hui, Zhang, Xiaowei, Li, Shuangli, Cheng, Xiaojie, Zhang, Xiuqing, Liu, Bin, Zeng, Changqing, Li, Songgang, Tan, Xuehai, Liu, Siqi, Dong, Wei, Wang, Jun, Wong, Gane Ka-Shu, Yu, Jun, Wang, Jian, Zhu, Qingyu, and Yang, Huanming

Published
2003
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38. Development of an ELISA-array for simultaneous detection of five encephalitis viruses

Author

Kang Xiaoping, Li Yuchang, Fan Li, Lin Fang, Wei Jingjing, Zhu Xiaolei, Hu Yi, Li Jing, Chang Guohui, Zhu Qingyu, Liu Hong, and Yang Yinhui

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use.

Published
2012
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39. Virulence of H5N1 virus in mice attenuates after in vitro serial passages

Author

Li Yongqiang, Xia Yukun, Zhan Dawei, Hu Yi, Chang Guohui, Liu Bohua, Li Jing, Yang Yinhui, and Zhu Qingyu

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract The virulence of A/Vietnam/1194/2004 (VN1194) in mice attenuated after serial passages in MDCK cells and chicken embryos, because the enriched large-plaque variants of the virus had significantly reduced virulence. In contrast, the small-plaque variants of the virus and the variants isolated from the brain of mice that were infected with the parental virus VN1194 had much higher virulence in mice. The virulence attenuation of serially propagated virus may be caused by the reduced neurotropism in mice. Our whole genome sequence analysis revealed substitutions of a total of two amino acids in PB1, three in PB2, two in PA common for virulence attenuated variants, all or part of which may be correlated with the virulence attenuation and reduced neurotropism of the serially propagated VN1194 in mice. Our study indicates that serial passages of VN1194 in vitro lead to adaptation and selection of variants that have markedly decreased virulence and neurotropism, which emphasizes the importance of direct analysis of original or less propagated virus samples.

Published
2011
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40. A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses

Author

Chang Guohui, Sun Tingting, Cai Xuyu, Lin Fang, Liu Hong, Li Yuchang, Kang Xiaoping, Zhu Qingyu, and Yang Yinhui

Subjects
Infectious and parasitic diseases, RC109-216
Abstract

Abstract In order to establish an accurate, ready-to-use assay for simultaneous detection of Eastern equine encephalitis virus (EEEV) and Western equine encephalitis virus (WEEV), we developed one duplex TaqMan real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay, which can be used in human and vector surveillance. First, we selected the primers and FAM-labeled TaqMan-probe specific for WEEV from the consensus sequence of NSP3 and the primers and HEX-labeled TaqMan-probe specific for EEEV from the consensus sequence of E3, respectively. Then we constructed and optimized the duplex real-time RT-PCR assay by adjusting the concentrations of primers and probes. Using a series of dilutions of transcripts containing target genes as template, we showed that the sensitivity of the assay reached 1 copy/reaction for EEEV and WEEV, and the performance was linear within the range of at least 106 transcript copies. Moreover, we evaluated the specificity of the duplex system using other encephalitis virus RNA as template, and found no cross-reactivity. Compared with virus isolation, the gold standard, the duplex real time RT-PCR assay we developed was 10-fold more sensitive for both WEEV and EEEV detection.

Published
2010
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41. Development and Evaluation of a Rapid and Sensitive EBOV-RPA Test for Rapid Diagnosis of Ebola Virus Disease

Author

Yang, Mingjuan, primary, Ke, Yuehua, additional, Wang, Xuesong, additional, Ren, Hang, additional, Liu, Wei, additional, Lu, Huijun, additional, Zhang, Wenyi, additional, Liu, Shiwei, additional, Chang, Guohui, additional, Tian, Shuguang, additional, Wang, Lihua, additional, Huang, Liuyu, additional, Liu, Chao, additional, Yang, Ruifu, additional, and Chen, Zeliang, additional

Published
2016
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