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Introduction: Antibiotic resistance has now become a perpetual global problem due to the emergence and reemergence of old and new multiresistant bacteria that there is a constant need of newer drugs to combat this phenomenon. Nanoparticles are now considered for their antibacterial effect and those such as Titanium dioxide have a good mechanical properties and biocompatibility, especially being non toxic to human skin and cost effective. This study was done to show the antibacterial effect of Titanium dioxide with and without Silver as addendum on Staphylococcus aureus strains from clinical samples. Materials and Methods: Staphylococcus aureus isolated from various clinical samples was inoculated onto peptone water and further inoculated onto glass slides coated with TiO2 annealed at 200C, 400C and onto TiO2 with 0.1%, 0.3%, 0.6% and 0.8% silver as additive at 1 hour intervals each. The growth was observed after 18 hours of incubation. Results: The highest antibacterial effect was observed within 2 hours of treatment with 0.8% Ag on TiO2 nanoparticles, while it took longer with the other concentrations of silver. With TiO2 and 0.1% Ag, it took at least 7 hours of treatment for complete antibacterial effect though within the first hour itself the effect was observed. Conclusion: Though TiO2 nanoparticles in pure form have significant antibacterial effect, there is a considerable increase in its antibacterial effect when silver is added as an additive, with higher concentrations of Ag having more effect. Keywords: Titanium dioxide nanoparticles, Silver additive, Antibacterial effect.