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139 results on '"Chan, Woon-Khiong"'

1. The role of Aquaporins in tumorigenesis: implications for therapeutic development

Author

Arkadyuti Bhattacharjee, Ankit Jana, Swagato Bhattacharjee, Sankalan Mitra, Swagata De, Badrah S. Alghamdi, Mohammad Zubair Alam, Ahmad Bakur Mahmoud, Zainab Al Shareef, Wael M. Abdel-Rahman, Chan Woon-Khiong, Athanasios Alexiou, Marios Papadakis, and Ghulam Md Ashraf

Subjects
Aquaporins, Cancer, Tumours, Water channel, Carcinoma, Therapeutic target, Medicine, Cytology, QH573-671
Abstract

Abstract Aquaporins (AQPs) are ubiquitous channel proteins that play a critical role in the homeostasis of the cellular environment by allowing the transit of water, chemicals, and ions. They can be found in many different types of cells and organs, including the lungs, eyes, brain, glands, and blood vessels. By controlling the osmotic water flux in processes like cell growth, energy metabolism, migration, adhesion, and proliferation, AQPs are capable of exerting their regulatory influence over a wide range of cellular processes. Tumour cells of varying sources express AQPs significantly, especially in malignant tumours with a high propensity for metastasis. New insights into the roles of AQPs in cell migration and proliferation reinforce the notion that AQPs are crucial players in tumour biology. AQPs have recently been shown to be a powerful tool in the fight against pathogenic antibodies and metastatic cell migration, despite the fact that the molecular processes of aquaporins in pathology are not entirely established. In this review, we shall discuss the several ways in which AQPs are expressed in the body, the unique roles they play in tumorigenesis, and the novel therapeutic approaches that could be adopted to treat carcinoma.

Published
2024
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6. Upregulation of the JAK-STAT pathway promotes maturation of human embryonic stem cell-derived cardiomyocytes

Author

Ho, Beatrice Xuan, primary, Yu, Hongbing, additional, Pang, Jeremy Kah Sheng, additional, Hor, Jin-Hui, additional, Liew, Lee Chuen, additional, Szyniarowski, Piotr, additional, Lim, Christina Ying Yan, additional, An, Omer, additional, Yang, Henry He, additional, Stewart, Colin L., additional, Chan, Woon Khiong, additional, Ng, Shi-Yan, additional, and Soh, Boon-Seng, additional

Published
2021
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7. Hazard profiling of a combinatorial library of zinc oxide nanoparticles: Ameliorating light and dark toxicity through surface passivation

Author

Saji George, Hong Yin, Ziruo Liu, Shirley Shen, Ivan Cole, and Chan Woon Khiong

Subjects
Environmental Engineering, Health, Toxicology and Mutagenesis, Sunlight, Animals, Metal Nanoparticles, Nanoparticles, Environmental Chemistry, Zinc Oxide, Pollution, Waste Management and Disposal, Zebrafish
Abstract

Zinc oxide (ZnO) is one of the high-volume production nanoparticles (NPs) currently used in a wide range of consumer and industrial goods. The inevitable seepage into environmental matrices and the photoactive nature of ZnO NPs warrants hazard profiling under environmentally related conditions. In this paper, the influence of simulated solar light (SSL) on dissolution behaviour and phototoxicity of ZnO NPs was studied using a combinatorial library of ZnO NPs with different sizes, surface coatings, dopant chemistry, and aspect ratios in a fish cell line (BF2) and zebrafish embryos. Generally, the cytotoxicity and embryo mortality increased when exposed concomitantly to SSL and ZnO NPs. The increase in toxic potential of ZnO NPs during SSL exposure concurred with release of Zn ions and ROS generation. Surface modification of NPs with poly(methacrylic acid) (PMAA), silica or serum coating decreased toxicity and ZnO with serum coating was the only NP that had no significant effect on any of the cytotoxicity parameters when tested under both dark and SSL conditions. Results from our study show that exposure to light could increase the toxic potential of ZnO NPs to environmental lifeforms and mitigation of ZnO NP toxicity is possible through modifying the surface chemistry.

Published
2022

10. Generation of human chambered cardiac organoids from pluripotent stem cells for improved modelling of cardiovascular diseases

Author

Ho, Beatrice Xuan, primary, Pang, Jeremy, additional, Phua, Qian Hua, additional, Liew, Lee Chuen, additional, Poh, Boon Min, additional, Chen, Ying, additional, Loh, Yuin-Han, additional, An, Omer, additional, Yang, Henry He, additional, Seshachalam, Veerabrahma Pratap, additional, Koh, Judice LY, additional, Chan, Woon-Khiong, additional, Ng, Shi-Yan, additional, and Soh, Boon-Seng, additional

Published
2021
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11. Potential modes of COVID-19 transmission from human eye revealed by single-cell atlas

Author

Yuin-Han Loh, Katherine Anne Lau, Hu Li, Timothy A. Blenkinsop, Chan Woon Khiong, Kiyofumi Hamashima, and Pradeep Gautam

Subjects
Alveolar cells, Proteases, Cell type, medicine.anatomical_structure, Regulon, Transcription (biology), viruses, Cell, medicine, Biology, Receptor, Virus, Cell biology
Abstract

There is a pressing urgency to understand the entry route of SARS-CoV-2 viruses into the human body. SARS-CoV-2 viruses enter through ACE2 receptors after the S proteins of the virus are primed by proteases such as TMPRSS2. Most studies focused on the airway epithelial and lung alveolar cells as the route of infection, while the mode of transmission through the ocular route is not well established. Here, we profiled the presence of SARS-CoV-2 receptors and receptor-associated enzymes at single-cell resolution of thirty-three human ocular cell types. We identified unique populations of corneal cells with high ACE2 expression, among which the conjunctival cells co-expressed both ACE2 and TMPRSS2, suggesting that they could serve as the entry points for the virus. Integrative analysis further models the signaling and transcription regulon networks involved in the infection of distinct corneal cells. Our work constitutes a unique resource for the development of new treatments and management of COVID-19.

Published
2020

16. Re-entering the pluripotent state from blood lineage: promises and pitfalls of blood reprogramming

Author

Chen, Y., Yi, Yao, Xu, J., Chan, Woon-Khiong, Loh, Yuin-Han, Chen, Y., Yi, Yao, Xu, J., Chan, Woon-Khiong, and Loh, Yuin-Han

Abstract

Contains fulltext : 214027.pdf (publisher's version ) (Closed access), Blood reprogramming, in which induced pluripotent stem cells (iPSCs) are derived from haematopoietic lineages, has rapidly advanced over the past decade. Since the first report using human blood, haematopoietic cell types from various sources, such as the peripheral bone marrow and cord blood, have been successfully reprogrammed. The volume of blood required has also decreased, from around tens of millilitres to a single finger-prick drop. Besides, while early studies were limited to reprogramming methods relying on viral integration, nonintegrating reprogramming systems for blood lineages have been subsequently established. Together, these improvements have made feasible the future clinical applications of blood-derived iPSCs. Here, we review the progress in blood reprogramming from various perspectives, including the starting materials and subsequent reprogramming strategies. We also discuss the downstream applications of blood-derived iPSCs, highlighting their clinical value in terms of disease modelling and therapeutic development.

Published
2019

20. The Human Embryonic Stem Cells Transcriptome: How Much Do We Know?

Author

Wang Yue, Chak Li-Ling, Chan Woon-Khiong, Wong Pui Mun, Shubha Vij, Lai ZhenYang, and Ariff Bongso

Subjects
Genetics, Drug discovery, Biomedical Engineering, Biophysics, Bioengineering, Computational biology, Biology, Self renewal, Biochemistry, Embryonic stem cell, Transcriptome, embryonic structures, Stem cell, Gene, Biotechnology
Abstract

The establishment of human embryonic stem cell (hESC) lines in 1998 served to set the pace for understanding the molecular biology behind the two hallmark features of stem cells: self renewal and pluripotency. The excitement was generated in the hope that understanding the molecular biology of hESCs would provide a good model for studying early human development, disease and drug discovery and also hold the promise for providing a cure for degenerative human diseases. In spite of the large number of studies, the molecular basis of pluripotency has remained a matter of intrigue ever since the embryonic stem cells (ESCs) were first identified. A considerable percentage of these studies have been transcriptome-based. Interestingly, significant differences are seen not only between mouse and human ESC transcriptomes but also amongst the hESC studies. Nevertheless, a key set of pluripotency genes seem to be common, reinforcing the utility of transcriptome-based approaches in identifying the molecular basis of pluripotency in hESCs.

Published
2008

22. Immediate Digestion of Fish Muscle Following Field Collections Yields DNA Suitable for RAPD Fingerprinting

Author

Violet P. E. Phang, James Stuart Nelson, Chou Loke Ming, and Chan Woon Khiong

Subjects
Muscle Fibers, Skeletal, Fishes, DNA, Biology, DNA Fingerprinting, General Biochemistry, Genetics and Molecular Biology, Random Amplified Polymorphic DNA Technique, RAPD, chemistry.chemical_compound, Digestion (alchemy), Biochemistry, chemistry, Animals, %22">Fish , Food science, Biotechnology
Published
1997

26. Extraordinary salmon growth

Author

Devlin, Robert H., Yesaki, Timothy Y., Biagi, Carlo A., Donaldson, Edward M., Swanson, Penny, and Chan, Woon-Khiong

Subjects
Genetic code -- Physiological aspects, Salmon -- Genetic aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Studies of microinjection of linear pOnMTGH1 DNA, a genetic construct consisting of the metallothionein-B promoter coupled to the type-1 growth hormone gene, into the blastodisc area of coho salmon eggs whose growth is arrested following fertilization reveal that pOnMTGH1 construct is vital for growth acceleration. The transgenic fishes containing pOnMTGH1 formed a silver body coloration and were markedly larger than non-transgenic fishes. Transgenic fishes also exhibited high winter levels of growth hormone.

Published
1994

27. Tissue Expression Of The 6-Pyruvoyi Tetrahydropterin Synthase Gene In Adult Zebrafish,Danio Rerio.

Author

Kuah, Meng Kiat, Chan, Woon Khiong, Tengku Muhammad, Tengku Sifzizul, Chong, Alexander, Kuah, Meng Kiat, Chan, Woon Khiong, Tengku Muhammad, Tengku Sifzizul, and Chong, Alexander

Abstract

Teleost is one of the poikilotherms that can display different kind of colours on their body. This is due to the presence of different types of chromatophores, a group of pigment containing cells that is distributed in the epidermal layer of skin and scales.

Published
2004

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